Ddx58 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx58
Entrez Gene ID	297989
Full Name	DEXD/H-box helicase 58
General protein information	Preferred Names DEXD/H-box helicase 58 Names probable ATP-dependent RNA helicase DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106645.1	NP_001100115.1	probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ddx58	NP_001100115.1
Danio rerio (zebrafish)	LOC100333797	XP_002666571.3

Gene Ontology
Bibliography

Related articles in PubMed

A non-canonical role of the p97 complex in RIG-I antiviral signaling.
Hao Q, Jiao S, Shi Z, Li C, Meng X, Zhang Z, Wang Y, Song X, Wang W, Zhang R, Zhao Y, Wong CC, Zhou Z
The EMBO journal34(23)2903-20(2015 Dec)

Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I.
Peisley A, Wu B, Xu H, Chen ZJ, Hur S
Nature509(7498)110-4(2014 May)

TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro.
Sathe A, Reddy KV
PloS one9(1)e83882(2014)

LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.
Li Y, Chen R, Zhou Q, Xu Z, Li C, Wang S, Mao A, Zhang X, He W, Shu HB
Proceedings of the National Academy of Sciences of the United States of America109(29)11770-5(2012 Jul)

MDA5 cooperatively forms dimers and ATP-sensitive filaments upon binding double-stranded RNA.
Berke IC, Modis Y
The EMBO journal31(7)1714-26(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04886	NM_001106645.1 Latest version!	Rattus norvegicus DEXD/H-box helicase 58 (Ddx58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx58 ( NM_001106645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100115.1 Rattus norvegicus DEXD/H-box helicase 58 (Ddx58), mRNA.

Price & Availability↑

CloneID	ORa04886
Clone ID Related Accession (Same CDS sequence)	NM_001106645.1
Accession Version	NM_001106645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1507305600000
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473962.1. On or before Oct 4, 2007 this sequence version replaced XM_001067411.1, XM_216380.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01906358 [ECO:0000350] ##Evidence-Data-END##

ATGACCGCAG AGCAGCGGCA GAATCTGCAA GCATTCAGAG ACTATATCAA GAAGATTCTG 
GACCCCACCT ACATCCTCAG CTATATGAGT TCCTGGCTCC GGGATGATGA GGTGCAGTGC 
ATTCAGGCCG AGAAGAACAA CAAGGGCCCA ATAGAGGCCG CCTCACTGTT CCTCCGGTAC 
CTGTTGGAGC TGCAGACAGA GGGCTGGTTC CGGGCCTTTT TGGATGCCCT GTACCATGCA 
GGTTACTGTG GACTTTATGA AGCCATCGAA ACTTGGGACT TTCAAAAACT TGAAAAGTTA 
GAGGAACACA GATTACTTTT AAGACGTTTA GAGCCGGAAT TTAAGGCCAC AGTTAGCCCG 
ACAGATATCC TTTCTGAAAT ATCTGAATGT TTGATTAATC AGGAATGTGA AGAAATCAGA 
CAGATCTGCT TCACTAAAGG GAGGATGGCG GGTGCGGAGA AGATGGTCCA ATGTCTTCTC 
AGATCCGATA AGGAGAACTG GCCAAAGGTT TTGCAGTTTG CTTTGGAGAA AGACAACAGC 
AAGTTTAGTG AACTGTGGCT CGTCGATAAA GGTGTCAGAA GGGTTGACTG TAAGGCTGTT 
GAGGATGACG GAACAGAGAT GGCCAGCATA CAGATTTTCA TTCAGGAAGA ACCGGAGTGT 
CAGAATCTCA GTCAGAATCC CGGCCCTCCT TCAGAAGCGT CTCCTAATAA TTCACACAGC 
CCATTGAAAC CAAGAAATTA CCAACTGGAG CTTGCCCTGC CTGCCAAGAA AGGGAAAAAT 
ACAGTAATAT GTGCTCCTAC TGGTTGTGGA AAAACCTTTG TGTCGCTTCT CATATGCGAA 
CATCATCTTA AAAACTTTCC GCACGGACAG AAAGGGAAAG TGGTCTTTTT TGCTAACCAG 
ATCCCTGTGT ATGAACAGCA GGCCACTGTG TTCTCACGAT ATTTCGAAAG ATTTGGGTAC 
AATGTCGCCG GCATTTCGGG GGCAACGGCT GATAATGTGT CAGTGCAGCA CATCATTGAA 
AACAATGACA TCGTCATCCT GACACCCCAG ATTCTTGTGA ACAGTCTCAA CAATGGAGCC 
ATCCCCTCGC TGTCTGTCTT CACTCTGATG ATATTCGACG AGTGTCATAA CACCAGCAAA 
CACCACCCGT ACAACCAGAT CATGTTCAGA TACCTAGATC ACAAACTCGG AGGGTCACCC 
GACCCACTGC CTCAGGTCAT TGGGCTGACT GCTTCCGTTG GTGTTGGAGA TGCTAAAACC 
GTGGAGGAAG CCATGCTGCA CATCTGCAAA CTCTGTGCCG CCCTCGATGC CTCAGTGATT 
GCCACAGTCA GAGACAACGT GGAAGAACTG GAGCAGGTTG TTTATAAGCC TCAGAAAATT 
TCCAGGAAAG TGGAATCACG GGCTTCGAAC ACGTTTAAAT GCATCATATC TCAGCTGATG 
AGGGAGGCAG AGAGGCTGGC GAAGAACGTC TCTGAGGAAC TTGGAAAGTT CTTTCAAATT 
CAAAATCGAG AATTTGGCAC CCAGAAATAT GAGCAGTGGA TTGTCGCCGT TCACAAAGCA 
TGCACGGTGT TTCAGATGCC TGACAAAGAT GAGGAGAGCA GGATCTGCAA GGCGCTCTTT 
CTGTACACCT CCCACTTGCG GAAATACAAC GACGCCCTCA TCATCAGCGA GGATGCACAG 
ATGAAAGACG CTCTAAATTA CCTCAAAGCC TTCTTCCATG ATGTCCGAGA AGCAGCATTT 
GATGAGACTG AGCAAGATCT TACGCGGAGG TTTGAAGGCT TTGAAGAAAT GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100115.1
CDS	127..1923
Translation	MTAEQRQNLQAFRDYIKKILDPTYILSYMSSWLRDDEVQCIQAEKNNKGPIEAASLFLRYLLELQTEGWFRAFLDALYHAGYCGLYEAIETWDFQKLEKLEEHRLLLRRLEPEFKATVSPTDILSEISECLINQECEEIRQICFTKGRMAGAEKMVQCLLRSDKENWPKVLQFALEKDNSKFSELWLVDKGVRRVDCKAVEDDGTEMASIQIFIQEEPECQNLSQNPGPPSEASPNNSHSPLKPRNYQLELALPAKKGKNTVICAPTGCGKTFVSLLICEHHLKNFPHGQKGKVVFFANQIPVYEQQATVFSRYFERFGYNVAGISGATADNVSVQHIIENNDIVILTPQILVNSLNNGAIPSLSVFTLMIFDECHNTSKHHPYNQIMFRYLDHKLGGSPDPLPQVIGLTASVGVGDAKTVEEAMLHICKLCAALDASVIATVRDNVEELEQVVYKPQKISRKVESRASNTFKCIISQLMREAERLAKNVSEELGKFFQIQNREFGTQKYEQWIVAVHKACTVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEDAQMKDALNYLKAFFHDVREAAFDETEQDLTRRFEGFEEMD

Target ORF information:

RefSeq Version	NM_001106645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEXD/H-box helicase 58 (Ddx58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106645.1

ORF Insert Sequence:

ATGACCGCAG AGCAGCGGCA GAATCTGCAA GCATTCAGAG ACTATATCAA GAAGATTCTG 
GACCCCACCT ACATCCTCAG CTATATGAGT TCCTGGCTCC GGGATGATGA GGTGCAGTGC 
ATTCAGGCCG AGAAGAACAA CAAGGGCCCA ATAGAGGCCG CCTCACTGTT CCTCCGGTAC 
CTGTTGGAGC TGCAGACAGA GGGCTGGTTC CGGGCCTTTT TGGATGCCCT GTACCATGCA 
GGTTACTGTG GACTTTATGA AGCCATCGAA ACTTGGGACT TTCAAAAACT TGAAAAGTTA 
GAGGAACACA GATTACTTTT AAGACGTTTA GAGCCGGAAT TTAAGGCCAC AGTTAGCCCG 
ACAGATATCC TTTCTGAAAT ATCTGAATGT TTGATTAATC AGGAATGTGA AGAAATCAGA 
CAGATCTGCT TCACTAAAGG GAGGATGGCG GGTGCGGAGA AGATGGTCCA ATGTCTTCTC 
AGATCCGATA AGGAGAACTG GCCAAAGGTT TTGCAGTTTG CTTTGGAGAA AGACAACAGC 
AAGTTTAGTG AACTGTGGCT CGTCGATAAA GGTGTCAGAA GGGTTGACTG TAAGGCTGTT 
GAGGATGACG GAACAGAGAT GGCCAGCATA CAGATTTTCA TTCAGGAAGA ACCGGAGTGT 
CAGAATCTCA GTCAGAATCC CGGCCCTCCT TCAGAAGCGT CTCCTAATAA TTCACACAGC 
CCATTGAAAC CAAGAAATTA CCAACTGGAG CTTGCCCTGC CTGCCAAGAA AGGGAAAAAT 
ACAGTAATAT GTGCTCCTAC TGGTTGTGGA AAAACCTTTG TGTCGCTTCT CATATGCGAA 
CATCATCTTA AAAACTTTCC GCACGGACAG AAAGGGAAAG TGGTCTTTTT TGCTAACCAG 
ATCCCTGTGT ATGAACAGCA GGCCACTGTG TTCTCACGAT ATTTCGAAAG ATTTGGGTAC 
AATGTCGCCG GCATTTCGGG GGCAACGGCT GATAATGTGT CAGTGCAGCA CATCATTGAA 
AACAATGACA TCGTCATCCT GACACCCCAG ATTCTTGTGA ACAGTCTCAA CAATGGAGCC 
ATCCCCTCGC TGTCTGTCTT CACTCTGATG ATATTCGACG AGTGTCATAA CACCAGCAAA 
CACCACCCGT ACAACCAGAT CATGTTCAGA TACCTAGATC ACAAACTCGG AGGGTCACCC 
GACCCACTGC CTCAGGTCAT TGGGCTGACT GCTTCCGTTG GTGTTGGAGA TGCTAAAACC 
GTGGAGGAAG CCATGCTGCA CATCTGCAAA CTCTGTGCCG CCCTCGATGC CTCAGTGATT 
GCCACAGTCA GAGACAACGT GGAAGAACTG GAGCAGGTTG TTTATAAGCC TCAGAAAATT 
TCCAGGAAAG TGGAATCACG GGCTTCGAAC ACGTTTAAAT GCATCATATC TCAGCTGATG 
AGGGAGGCAG AGAGGCTGGC GAAGAACGTC TCTGAGGAAC TTGGAAAGTT CTTTCAAATT 
CAAAATCGAG AATTTGGCAC CCAGAAATAT GAGCAGTGGA TTGTCGCCGT TCACAAAGCA 
TGCACGGTGT TTCAGATGCC TGACAAAGAT GAGGAGAGCA GGATCTGCAA GGCGCTCTTT 
CTGTACACCT CCCACTTGCG GAAATACAAC GACGCCCTCA TCATCAGCGA GGATGCACAG 
ATGAAAGACG CTCTAAATTA CCTCAAAGCC TTCTTCCATG ATGTCCGAGA AGCAGCATTT 
GATGAGACTG AGCAAGATCT TACGCGGAGG TTTGAAGGCT TTGAAGAAAT GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A non-canonical role of the p97 complex in RIG-I antiviral signaling.
The EMBO journal34(23)2903-20(2015 Dec)
Hao Q,Jiao S,Shi Z,Li C,Meng X,Zhang Z,Wang Y,Song X,Wang W,Zhang R,Zhao Y,Wong CC,Zhou Z

Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I.
Nature509(7498)110-4(2014 May)
Peisley A,Wu B,Xu H,Chen ZJ,Hur S

TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro.
PloS one9(1)e83882(2014)
Sathe A,Reddy KV

LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.
Proceedings of the National Academy of Sciences of the United States of America109(29)11770-5(2012 Jul)
Li Y,Chen R,Zhou Q,Xu Z,Li C,Wang S,Mao A,Zhang X,He W,Shu HB

MDA5 cooperatively forms dimers and ATP-sensitive filaments upon binding double-stranded RNA.
The EMBO journal31(7)1714-26(2012 Apr)
Berke IC,Modis Y