Sart1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sart1
Entrez Gene ID	29678
Full Name	SART1, U4/U6.U5 tri-snRNP-associated protein 1
General protein information	Preferred Names SART1, U4/U6.U5 tri-snRNP-associated protein 1 Names U4/U6.U5 tri-snRNP-associated protein 1 SART-1 rSART-1 squamous cell carcinoma antigen recognized by T cells 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	gene expressed in the nucleus and upregulated in proliferating cells, also found in cytoplasm of epithelial cancer cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031596.1	NP_113784.1	U4/U6.U5 tri-snRNP-associated protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SART1	NP_005137.1
Danio rerio (zebrafish)	sart1	NP_001002673.1
Canis lupus familiaris (dog)	SART1	XP_005631804.1
Xenopus tropicalis (tropical clawed frog)	sart1	XP_002940547.2
Pan troglodytes (chimpanzee)	SART1	XP_003313187.1
Bos taurus (cattle)	SART1	NP_001180015.1
Rattus norvegicus (Norway rat)	Sart1	NP_113784.1
Mus musculus (house mouse)	Sart1	NP_058578.3

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Lührmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies.
Lemm I, Girard C, Kuhn AN, Watkins NJ, Schneider M, Bordonné R, Lührmann R
Molecular biology of the cell17(7)3221-31(2006 Jul)

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ
RNA (New York, N.Y.)8(4)426-39(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sart1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sart1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08210	NM_031596.1 Latest version!	Rattus norvegicus spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (Sart1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sart1 ( NM_031596.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113784.1 Rattus norvegicus spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (Sart1), mRNA.

Price & Availability↑

CloneID	ORa08210
Clone ID Related Accession (Same CDS sequence)	NM_031596.1
Accession Version	NM_031596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	U4/U6.U5 tri-snRNP-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB014722.1. ##Evidence-Data-START## Transcript exon combination :: AB014722.1, BC083551.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGTCGT CAAAGAAGCA CCGTGGAGAG AAGGAGGCAG CCGGGACTAC GGCTGCGGCC 
GGTACCGGGG GCACTACCGA GCAGCCGCCA CGGCATCGAG AGCACAAGAA ACACAAGCAT 
CGGAGCAGCG GCGGCGGCAG CAGCGGCGGC GAACGACGGA AGCGGAGCCG CGAGCGTGGG 
GCCGAGCGCG GGAGCGGGAG GCGCGGGGCC GAAGCCGAGG CCCGCAGCGG CGCGCATGGT 
CGTGAACGAA GCCAAGCGGA ACCCTCGGAG CGGCGCGTGA AGCGGGAGAA ACGCGATGAA 
GGCTATGAGG CCGCTGCCAG CTCCAAAGCC AGCTCTGGAG ATGCCTCATC ACTCAGCATC 
GAGGAGACCA ATAAACTCCG AGCAAAGTTG GGGTTGAAGC CCTTGGAAGT CAATGCTGTT 
AAGAAAGAGG CGGGCACCAA GGAGGAGCCC GTGGCAGCCG ATGTTATCAA CCCCATGGCC 
TTGCGACAGC GTGAAGAACT ACGGGAGAAG CTGGCAGCTG CCAAGGAAAA GCGCTTGCTG 
AACCAAAAAT TGGGGAAAAT AAAGACACTG GGGGAGGATG ACCCCTGGCT TGATGACACT 
GCAGCCTGGA TAGAGAGGAG CCGGCAGCTA CAGAAAGAGA AGGACTTGGC GGAGAAGCGG 
GCCAAGCTGC TAGAAGAGAT GGACCAAGAG TTTGGTGTGA GCACTTTAGT GGAGGAGGAG 
TTTGAGCAGA GGCGACAGGA CCTGTACAGC GCCCGGGACT TGCAAGGCCT CACCGTGGAA 
CATGCAATCG ATTCCTTTCG AGAAGGGGAG ACTGTGGTCC TCACTCTCAA GGACAAAGGG 
GTTCTGCAGG AGGGGGAGGA TGTGCTGGTG AATGTGAACA TGGTGGACAA GGAGCGAGCA 
GACAAGAACG TGGAACTTCG GAAGAAGAAG CCTGACTACC TGCCCTATGT GGAGGACGAG 
AGTGTGGATG ACTTGGCACA GCAAAAACCT CGCTCTATCC TGGCCAAATA TGATGAGGAA 
CTGGAGGGCG AGCGGCCACA TTCCTTCCGT TTAGAGCAGG GTGGCATGGC CGATGGCCTG 
AGAGAACGAG AGCTCGAAGA AATCCGGACC AAGCTACGGC TGCAGGCTCA GTCTCTGAAC 
ACTGTAGGGC CCCGGCTTGC CTCTGAGTAC CTCAGTCCGG AGGAGATGGT GACTTTCAAA 
AAGACCAAAC GGAGAGTGAA GAAAATCCGA AAGAAGGAGA AGGAGGTAAT AATGCGGGCA 
GATGACTTGC TGCCCCTTGG CGAAGACCAG ACTCAGGACG GGGACTTTGG ATCCAGGCTT 
CGGGGCCGGG GTCGGCGCCG AGTTCCTGAA GTGGAGGAGG AGGCCCTTGA GGATGAAGAG 
AAGGACCCTG TGGCCCAGCC GCCACCATCA GATGACACTC GCGTAGAGAA CATGGACATC 
AGTGATGAGG AGGATGGGGG GGCTCTTCCC TCGGGGCCCC CAGAGCTGGA GGAGGACGAA 
GCGGAGCTGG AGCTGCAGAA GCAGCTGGAG AAGGGGCGCC GGCTGCGGCA GCTGCAGCAA 
CTGCAGCAGC TGCGGGACAG CGGTGAGAAG GTGCTGGAGA TTGTGAAGAA ACTGGAGTCT 
CGCCAACGTG GCTGGGAAGA GGAAGAGGAT CCTGAGCGGA AGGGGACCAT CGTGTTCAAT 
GCCACTTCTG AGTTCTGTCG CACTCTGGGG GAAATCCCCA CTTACGGATT AGCTGGCAAC 
CGAGAGGAGC AGGAGGAGCT CATGGACTTT GAAAGGGATG AAGAGCGCTC GGCCAATGGT 
GGCTCTGAAT CAGATGGCGA AGAGAACATT GGCTGGAGCA CCGTCAACCT GGATGAGGAG 
AAGCAACATC AGGATTTCTC CGCTTCCTCT ACCACCATCC TGGATGAAGA GCCCATTGTG 
AACAGAGGGC TGGCGGCTGC CCTGCTCCTG TGTCAGAACA AAGGACTGTT GGAGACCACG 
GTACAAAAGG TGGCCCGAGT AAAGGCTCCC AATAAGTCAC TGCCTTCAGC AGTGTACTGC 
ATCGAGGATA AGATGGCCAT TGATGACAAG TACAGCCGAC GTGAGGAGTA CAGGGGCTTC 
ACCCAGGACT TCAAGGAGAA GGATGGCTAC AAGCCCGATG TTAAGATTGA ATATGTGGAT 
GAGACGGGCA GGAAACTGAC TCCCAAGGAG GCATTCCGGC AGCTGTCCCA CCGCTTCCAT 
GGGAAGGGCT CAGGGAAGAT GAAGACTGAG CGGCGGATGA AGAAGCTGGA TGAGGAGGCG 
CTCCTAAAGA AAATGAGTTC CAGTGACACG CCCCTGGGAA CCGTGGCACT GCTCCAGGAG 
AAGCAGAAGG CTCAAAAGAC ACCGTACATC GTGCTCAGTG GCAGTGGCAA GAGCATGAAT 
GCGAACACCA TCACCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113784.1
CDS	59..2479
Translation	MGSSKKHRGEKEAAGTTAAAGTGGTTEQPPRHREHKKHKHRSSGGGSSGGERRKRSRERGAERGSGRRGAEAEARSGAHGRERSQAEPSERRVKREKRDEGYEAAASSKASSGDASSLSIEETNKLRAKLGLKPLEVNAVKKEAGTKEEPVAADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVLQEGEDVLVNVNMVDKERADKNVELRKKKPDYLPYVEDESVDDLAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIRTKLRLQAQSLNTVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEKEVIMRADDLLPLGEDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENMDISDEEDGGALPSGPPELEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKLESRQRGWEEEEDPERKGTIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQHQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	NM_031596.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (Sart1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031596.1

ORF Insert Sequence:

ATGGGGTCGT CAAAGAAGCA CCGTGGAGAG AAGGAGGCAG CCGGGACTAC GGCTGCGGCC 
GGTACCGGGG GCACTACCGA GCAGCCGCCA CGGCATCGAG AGCACAAGAA ACACAAGCAT 
CGGAGCAGCG GCGGCGGCAG CAGCGGCGGC GAACGACGGA AGCGGAGCCG CGAGCGTGGG 
GCCGAGCGCG GGAGCGGGAG GCGCGGGGCC GAAGCCGAGG CCCGCAGCGG CGCGCATGGT 
CGTGAACGAA GCCAAGCGGA ACCCTCGGAG CGGCGCGTGA AGCGGGAGAA ACGCGATGAA 
GGCTATGAGG CCGCTGCCAG CTCCAAAGCC AGCTCTGGAG ATGCCTCATC ACTCAGCATC 
GAGGAGACCA ATAAACTCCG AGCAAAGTTG GGGTTGAAGC CCTTGGAAGT CAATGCTGTT 
AAGAAAGAGG CGGGCACCAA GGAGGAGCCC GTGGCAGCCG ATGTTATCAA CCCCATGGCC 
TTGCGACAGC GTGAAGAACT ACGGGAGAAG CTGGCAGCTG CCAAGGAAAA GCGCTTGCTG 
AACCAAAAAT TGGGGAAAAT AAAGACACTG GGGGAGGATG ACCCCTGGCT TGATGACACT 
GCAGCCTGGA TAGAGAGGAG CCGGCAGCTA CAGAAAGAGA AGGACTTGGC GGAGAAGCGG 
GCCAAGCTGC TAGAAGAGAT GGACCAAGAG TTTGGTGTGA GCACTTTAGT GGAGGAGGAG 
TTTGAGCAGA GGCGACAGGA CCTGTACAGC GCCCGGGACT TGCAAGGCCT CACCGTGGAA 
CATGCAATCG ATTCCTTTCG AGAAGGGGAG ACTGTGGTCC TCACTCTCAA GGACAAAGGG 
GTTCTGCAGG AGGGGGAGGA TGTGCTGGTG AATGTGAACA TGGTGGACAA GGAGCGAGCA 
GACAAGAACG TGGAACTTCG GAAGAAGAAG CCTGACTACC TGCCCTATGT GGAGGACGAG 
AGTGTGGATG ACTTGGCACA GCAAAAACCT CGCTCTATCC TGGCCAAATA TGATGAGGAA 
CTGGAGGGCG AGCGGCCACA TTCCTTCCGT TTAGAGCAGG GTGGCATGGC CGATGGCCTG 
AGAGAACGAG AGCTCGAAGA AATCCGGACC AAGCTACGGC TGCAGGCTCA GTCTCTGAAC 
ACTGTAGGGC CCCGGCTTGC CTCTGAGTAC CTCAGTCCGG AGGAGATGGT GACTTTCAAA 
AAGACCAAAC GGAGAGTGAA GAAAATCCGA AAGAAGGAGA AGGAGGTAAT AATGCGGGCA 
GATGACTTGC TGCCCCTTGG CGAAGACCAG ACTCAGGACG GGGACTTTGG ATCCAGGCTT 
CGGGGCCGGG GTCGGCGCCG AGTTCCTGAA GTGGAGGAGG AGGCCCTTGA GGATGAAGAG 
AAGGACCCTG TGGCCCAGCC GCCACCATCA GATGACACTC GCGTAGAGAA CATGGACATC 
AGTGATGAGG AGGATGGGGG GGCTCTTCCC TCGGGGCCCC CAGAGCTGGA GGAGGACGAA 
GCGGAGCTGG AGCTGCAGAA GCAGCTGGAG AAGGGGCGCC GGCTGCGGCA GCTGCAGCAA 
CTGCAGCAGC TGCGGGACAG CGGTGAGAAG GTGCTGGAGA TTGTGAAGAA ACTGGAGTCT 
CGCCAACGTG GCTGGGAAGA GGAAGAGGAT CCTGAGCGGA AGGGGACCAT CGTGTTCAAT 
GCCACTTCTG AGTTCTGTCG CACTCTGGGG GAAATCCCCA CTTACGGATT AGCTGGCAAC 
CGAGAGGAGC AGGAGGAGCT CATGGACTTT GAAAGGGATG AAGAGCGCTC GGCCAATGGT 
GGCTCTGAAT CAGATGGCGA AGAGAACATT GGCTGGAGCA CCGTCAACCT GGATGAGGAG 
AAGCAACATC AGGATTTCTC CGCTTCCTCT ACCACCATCC TGGATGAAGA GCCCATTGTG 
AACAGAGGGC TGGCGGCTGC CCTGCTCCTG TGTCAGAACA AAGGACTGTT GGAGACCACG 
GTACAAAAGG TGGCCCGAGT AAAGGCTCCC AATAAGTCAC TGCCTTCAGC AGTGTACTGC 
ATCGAGGATA AGATGGCCAT TGATGACAAG TACAGCCGAC GTGAGGAGTA CAGGGGCTTC 
ACCCAGGACT TCAAGGAGAA GGATGGCTAC AAGCCCGATG TTAAGATTGA ATATGTGGAT 
GAGACGGGCA GGAAACTGAC TCCCAAGGAG GCATTCCGGC AGCTGTCCCA CCGCTTCCAT 
GGGAAGGGCT CAGGGAAGAT GAAGACTGAG CGGCGGATGA AGAAGCTGGA TGAGGAGGCG 
CTCCTAAAGA AAATGAGTTC CAGTGACACG CCCCTGGGAA CCGTGGCACT GCTCCAGGAG 
AAGCAGAAGG CTCAAAAGAC ACCGTACATC GTGCTCAGTG GCAGTGGCAA GAGCATGAAT 
GCGAACACCA TCACCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,Lührmann R,Stark H

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies.
Molecular biology of the cell17(7)3221-31(2006 Jul)
Lemm I,Girard C,Kuhn AN,Watkins NJ,Schneider M,Bordonné R,Lührmann R

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.
RNA (New York, N.Y.)8(4)426-39(2002 Apr)
Jurica MS,Licklider LJ,Gygi SR,Grigorieff N,Moore MJ

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Sart1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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