Sart1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sart1
Entrez Gene ID	20227
Full Name	squamous cell carcinoma antigen recognized by T cells 1
Synonyms	U5-110K
General protein information	Preferred Names squamous cell carcinoma antigen recognized by T cells 1 Names U4/U6.U5 tri-snRNP-associated protein 1 SART-1 hypoxia-associated factor mSART-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_030250798.1	XP_030106658.1	U4/U6.U5 tri-snRNP-associated protein 1 isoform X1
NM_016882.3	NP_058578.3	U4/U6.U5 tri-snRNP-associated protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SART1	NP_005137.1
Danio rerio (zebrafish)	sart1	NP_001002673.1
Canis lupus familiaris (dog)	SART1	XP_005631804.1
Xenopus tropicalis (tropical clawed frog)	sart1	XP_002940547.2
Pan troglodytes (chimpanzee)	SART1	XP_003313187.1
Bos taurus (cattle)	SART1	NP_001180015.1
Rattus norvegicus (Norway rat)	Sart1	NP_113784.1
Mus musculus (house mouse)	Sart1	NP_058578.3

Gene Ontology
Bibliography

Related articles in PubMed

A new HIF-1α/RANTES-driven pathway to hepatocellular carcinoma mediated by germline haploinsufficiency of SART1/HAF in mice.
Koh MY, Gagea M, Sargis T, Lemos R Jr, Grandjean G, Charbono A, Bekiaris V, Sedy J, Kiriakova G, Liu X, Roberts LR, Ware C, Powis G
Hepatology (Baltimore, Md.)63(5)1576-91(2016 May)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Epitope tagging of proteins at the native chromosomal loci of genes in mice and in cultured vertebrate cells.
Chen YI, Maika SD, Stevens SW
Journal of molecular biology361(3)412-9(2006 Aug)

Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis.
Bolland DJ, Hewitt JE
Gene271(1)43-9(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sart1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sart1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10686	NM_016882.3 Latest version!	Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87502	XM_030250798.1 Latest version!	Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sart1 ( NM_016882.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058578.3 Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), mRNA.

Price & Availability↑

CloneID	OMu10686
Clone ID Related Accession (Same CDS sequence)	NM_016882.3
Accession Version	NM_016882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	U4/U6.U5 tri-snRNP-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122861.4 and AB014721.1. On Jan 26, 2007 this sequence version replaced NM_016882.2. ##Evidence-Data-START## Transcript exon combination :: AF129931.1, SRR1660819.80090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGGTCGT CAAAGAAGCA CCGTGGCGAG AAGGAGGCGG CCGGGACGAC GGCTGCGGCC 
GGGACTGGGG GCACGACCGA GCAGCCGCCA CGGCATCGCG AGCATAAGAA ACACAAGCAC 
CGGAGCAGCG GCGGCGGCAG CAGCGGCGGC GAACGACGGA AGCGGAGCCG GGAGCGTGGG 
AGTGAGCGCG GGAGCGGGCG GCGTGGGGCG GAAGCCGAGG CCCGCAGCGG CGCGCACGGT 
CGCGAACGAA GCCAAGCGGA GCCCTCGGAG CGGCGCGTGA AGCGGGAGAA ACGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAGCC AGCTCTGGCG ATGCCTCATC CCTCAGCATT 
GAGGAGACCA ATAAACTCCG AGCAAAGTTG GGGCTGAAGC CCTTGGAGGT CAATGCTGTT 
AAGAAAGAGG CGGGCACCAA GGAGGAGCCC GTGGCAGCCG ATGTTATCAA CCCCATGGCC 
TTGCGACAGC GGGAAGAACT GCGGGAGAAG CTGGCAGCTG CCAAGGAAAA ACGCTTGCTG 
AACCAAAAAT TGGGGAAAAT AAAGACACTG GGGGAGGATG ACCCCTGGCT GGATGATACT 
GCAGCCTGGA TAGAGAGGAG CCGGCAGCTA CAGAAAGAGA AGGACCTGGC AGAGAAGCGG 
GCTAAGCTAC TAGAGGAGAT GGACCAAGAG TTTGGTGTGA GCACTTTAGT GGAGGAGGAG 
TTTGAGCAGA GGCGACAGGA CCTGTACAGT GCCCGGGACC TGCAAGGCCT CACTGTGGAA 
CATGCCATCG ATTCTTTTCG AGAAGGGGAG ACTGTGGTCC TCACCCTCAA GGACAAAGGG 
GTACTGCAGG ACGGGGAGGA CGTGCTGGTG AATGTGAACA TGGTGGACAA GGAGCGGGCG 
GACAAGAACG TGGAGCTTCG GAAAAAGAAG CCTGACTACC TGCCCTATGC GGAAGATGAG 
AGTGTGGACG ATTTGGCACA GCAAAAACCT CGTTCTATCC TGGCCAAATA TGATGAGGAA 
CTGGAGGGCG AGCGACCACA TTCCTTCCGT CTGGAGCAGG GTGGGATGGC CGATGGCCTG 
AGGGAACGAG AGTTAGAAGA AATCCGCACC AAGCTACGGC TGCAGGCTCA GTCTCTGAGC 
TCCGTAGGGC CCCGGCTTGC CTCTGAGTAC CTCAGTCCCG AGGAGATGGT GACCTTCAAG 
AAGACCAAAC GGAGAGTGAA GAAAATCCGA AAGAAGGAGA AGGAGGTGAT AATGCGGGCA 
GATGACTTGC TGCCCCTGGG CGACCAGACT CAGGATGGGG ACTTTGGATC CAGGCTCCGG 
GGCCGGGGTC GGCGCCGAGT TCCTGAGGTG GAGGAGGAGG CCCTTGAGGA TGAGGAGAAG 
GACCCTGTGG CCCAGCCGCC ACCATCGGAT GACACTCGAG TAGAGAACAT GGACATCAGT 
GATGAAGAGG ATGGGGGAGC TCTTCCGCCA GGGTCCCCAG AGGGGCTGGA GGAAGACGAG 
GCAGAGCTGG AGCTGCAGAA GCAGCTGGAG AAGGGGCGCC GGCTGCGGCA GCTGCAGCAA 
CTGCAGCAGC TCCGAGACAG TGGTGAGAAG GTGCTGGAGA TTGTGAAGAA ACTGGAGTCT 
CGCCAGCGCG GCTGGGAGGA GGAGGAGGAC CCTGAGAGGA AGGGGACCAT CGTGTTCAAT 
GCCACTTCTG AATTCTGCCG CACGCTGGGG GAGATCCCCA CCTACGGGTT GGCTGGCAAC 
CGCGAGGAGC AGGAAGAGCT CATGGACTTT GAAAGGGATG AGGAGCGCTC GGCCAACGGT 
GGCTCTGAAT CAGATGGGGA AGAGAACATT GGCTGGAGCA CCGTCAACCT GGATGAGGAG 
AAGCAGCATC AGGATTTCTC TGCTTCCTCT ACCACCATCC TGGATGAGGA GCCCATCGTG 
AACAGAGGGC TGGCTGCTGC CCTGCTCCTG TGTCAGAACA AAGGACTGCT GGAGACCACA 
GTACAAAAGG TGGCCCGGGT GAAGGCTCCC AATAAGTCAC TGCCCTCAGC AGTGTACTGC 
ATCGAGGATA AGATGGCCAT TGATGACAAG TACAGCCGAC GGGAGGAGTA CAGGGGCTTC 
ACCCAGGACT TCAAGGAGAA AGATGGCTAC AAGCCTGATG TTAAGATTGA ATACGTGGAT 
GAGACGGGCA GGAAACTGAC TCCCAAGGAG GCATTCCGGC AGCTGTCCCA CCGCTTCCAC 
GGAAAGGGCT CAGGCAAGAT GAAGACTGAG CGGCGGATGA AGAAGCTGGA TGAGGAGGCG 
CTCCTGAAGA AAATGAGCTC CAGTGACACG CCCCTGGGCA CCGTGGCACT GCTCCAGGAG 
AAGCAGAAGG CCCAGAAGAC ACCGTATATC GTGCTCAGTG GCAGTGGCAA GAGCATGAAT 
GCGAACACCA TCACCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058578.3
CDS	77..2497
Translation	MGSSKKHRGEKEAAGTTAAAGTGGTTEQPPRHREHKKHKHRSSGGGSSGGERRKRSRERGSERGSGRRGAEAEARSGAHGRERSQAEPSERRVKREKRDDGYEAAASSKASSGDASSLSIEETNKLRAKLGLKPLEVNAVKKEAGTKEEPVAADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVLQDGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIRTKLRLQAQSLSSVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEKEVIMRADDLLPLGDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENMDISDEEDGGALPPGSPEGLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKLESRQRGWEEEEDPERKGTIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQHQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	NM_016882.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016882.3

ORF Insert Sequence:

ATGGGGTCGT CAAAGAAGCA CCGTGGCGAG AAGGAGGCGG CCGGGACGAC GGCTGCGGCC 
GGGACTGGGG GCACGACCGA GCAGCCGCCA CGGCATCGCG AGCATAAGAA ACACAAGCAC 
CGGAGCAGCG GCGGCGGCAG CAGCGGCGGC GAACGACGGA AGCGGAGCCG GGAGCGTGGG 
AGTGAGCGCG GGAGCGGGCG GCGTGGGGCG GAAGCCGAGG CCCGCAGCGG CGCGCACGGT 
CGCGAACGAA GCCAAGCGGA GCCCTCGGAG CGGCGCGTGA AGCGGGAGAA ACGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAGCC AGCTCTGGCG ATGCCTCATC CCTCAGCATT 
GAGGAGACCA ATAAACTCCG AGCAAAGTTG GGGCTGAAGC CCTTGGAGGT CAATGCTGTT 
AAGAAAGAGG CGGGCACCAA GGAGGAGCCC GTGGCAGCCG ATGTTATCAA CCCCATGGCC 
TTGCGACAGC GGGAAGAACT GCGGGAGAAG CTGGCAGCTG CCAAGGAAAA ACGCTTGCTG 
AACCAAAAAT TGGGGAAAAT AAAGACACTG GGGGAGGATG ACCCCTGGCT GGATGATACT 
GCAGCCTGGA TAGAGAGGAG CCGGCAGCTA CAGAAAGAGA AGGACCTGGC AGAGAAGCGG 
GCTAAGCTAC TAGAGGAGAT GGACCAAGAG TTTGGTGTGA GCACTTTAGT GGAGGAGGAG 
TTTGAGCAGA GGCGACAGGA CCTGTACAGT GCCCGGGACC TGCAAGGCCT CACTGTGGAA 
CATGCCATCG ATTCTTTTCG AGAAGGGGAG ACTGTGGTCC TCACCCTCAA GGACAAAGGG 
GTACTGCAGG ACGGGGAGGA CGTGCTGGTG AATGTGAACA TGGTGGACAA GGAGCGGGCG 
GACAAGAACG TGGAGCTTCG GAAAAAGAAG CCTGACTACC TGCCCTATGC GGAAGATGAG 
AGTGTGGACG ATTTGGCACA GCAAAAACCT CGTTCTATCC TGGCCAAATA TGATGAGGAA 
CTGGAGGGCG AGCGACCACA TTCCTTCCGT CTGGAGCAGG GTGGGATGGC CGATGGCCTG 
AGGGAACGAG AGTTAGAAGA AATCCGCACC AAGCTACGGC TGCAGGCTCA GTCTCTGAGC 
TCCGTAGGGC CCCGGCTTGC CTCTGAGTAC CTCAGTCCCG AGGAGATGGT GACCTTCAAG 
AAGACCAAAC GGAGAGTGAA GAAAATCCGA AAGAAGGAGA AGGAGGTGAT AATGCGGGCA 
GATGACTTGC TGCCCCTGGG CGACCAGACT CAGGATGGGG ACTTTGGATC CAGGCTCCGG 
GGCCGGGGTC GGCGCCGAGT TCCTGAGGTG GAGGAGGAGG CCCTTGAGGA TGAGGAGAAG 
GACCCTGTGG CCCAGCCGCC ACCATCGGAT GACACTCGAG TAGAGAACAT GGACATCAGT 
GATGAAGAGG ATGGGGGAGC TCTTCCGCCA GGGTCCCCAG AGGGGCTGGA GGAAGACGAG 
GCAGAGCTGG AGCTGCAGAA GCAGCTGGAG AAGGGGCGCC GGCTGCGGCA GCTGCAGCAA 
CTGCAGCAGC TCCGAGACAG TGGTGAGAAG GTGCTGGAGA TTGTGAAGAA ACTGGAGTCT 
CGCCAGCGCG GCTGGGAGGA GGAGGAGGAC CCTGAGAGGA AGGGGACCAT CGTGTTCAAT 
GCCACTTCTG AATTCTGCCG CACGCTGGGG GAGATCCCCA CCTACGGGTT GGCTGGCAAC 
CGCGAGGAGC AGGAAGAGCT CATGGACTTT GAAAGGGATG AGGAGCGCTC GGCCAACGGT 
GGCTCTGAAT CAGATGGGGA AGAGAACATT GGCTGGAGCA CCGTCAACCT GGATGAGGAG 
AAGCAGCATC AGGATTTCTC TGCTTCCTCT ACCACCATCC TGGATGAGGA GCCCATCGTG 
AACAGAGGGC TGGCTGCTGC CCTGCTCCTG TGTCAGAACA AAGGACTGCT GGAGACCACA 
GTACAAAAGG TGGCCCGGGT GAAGGCTCCC AATAAGTCAC TGCCCTCAGC AGTGTACTGC 
ATCGAGGATA AGATGGCCAT TGATGACAAG TACAGCCGAC GGGAGGAGTA CAGGGGCTTC 
ACCCAGGACT TCAAGGAGAA AGATGGCTAC AAGCCTGATG TTAAGATTGA ATACGTGGAT 
GAGACGGGCA GGAAACTGAC TCCCAAGGAG GCATTCCGGC AGCTGTCCCA CCGCTTCCAC 
GGAAAGGGCT CAGGCAAGAT GAAGACTGAG CGGCGGATGA AGAAGCTGGA TGAGGAGGCG 
CTCCTGAAGA AAATGAGCTC CAGTGACACG CCCCTGGGCA CCGTGGCACT GCTCCAGGAG 
AAGCAGAAGG CCCAGAAGAC ACCGTATATC GTGCTCAGTG GCAGTGGCAA GAGCATGAAT 
GCGAACACCA TCACCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sart1 ( XM_030250798.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106658.1 Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87502
Clone ID Related Accession (Same CDS sequence)	XM_030250798.1
Accession Version	XM_030250798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	U4/U6.U5 tri-snRNP-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC GACAGCGGGA AGAACTGCGG GAGAAGCTGG CAGCTGCCAA GGAAAAACGC 
TTGCTGAACC AAAAATTGGG GAAAATAAAG ACACTGGGGG AGGATGACCC CTGGCTGGAT 
GATACTGCAG CCTGGATAGA GAGGAGCCGG CAGCTACAGA AAGAGAAGGA CCTGGCAGAG 
AAGCGGGCTA AGCTACTAGA GGAGATGGAC CAAGAGTTTG GTGTGAGCAC TTTAGTGGAG 
GAGGAGTTTG AGCAGAGGCG ACAGGACCTG TACAGTGCCC GGGACCTGCA AGGCCTCACT 
GTGGAACATG CCATCGATTC TTTTCGAGAA GGGGAGACTG TGGTCCTCAC CCTCAAGGAC 
AAAGGGGTAC TGCAGGACGG GGAGGACGTG CTGGTGAATG TGAACATGGT GGACAAGGAG 
CGGGCGGACA AGAACGTGGA GCTTCGGAAA AAGAAGCCTG ACTACCTGCC CTATGCGGAA 
GATGAGAGTG TGGACGATTT GGCACAGCAA AAACCTCGTT CTATCCTGGC CAAATATGAT 
GAGGAACTGG AGGGCGAGCG ACCACATTCC TTCCGTCTGG AGCAGGGTGG GATGGCCGAT 
GGCCTGAGGG AACGAGAGTT AGAAGAAATC CGCACCAAGC TACGGCTGCA GGCTCAGTCT 
CTGAGCTCCG TAGGGCCCCG GCTTGCCTCT GAGTACCTCA GTCCCGAGGA GATGGTGACC 
TTCAAGAAGA CCAAACGGAG AGTGAAGAAA ATCCGAAAGA AGGAGAAGGA GGTGATAATG 
CGGGCAGATG ACTTGCTGCC CCTGGGCGAC CAGACTCAGG ATGGGGACTT TGGATCCAGG 
CTCCGGGGCC GGGGTCGGCG CCGAGTTCCT GAGGTGGAGG AGGAGGCCCT TGAGGATGAG 
GAGAAGGACC CTGTGGCCCA GCCGCCACCA TCGGATGACA CTCGAGTAGA GAACATGGAC 
ATCAGTGATG AAGAGGATGG GGGAGCTCTT CCGCCAGGGT CCCCAGAGGG GCTGGAGGAA 
GACGAGGCAG AGCTGGAGCT GCAGAAGCAG CTGGAGAAGG GGCGCCGGCT GCGGCAGCTG 
CAGCAACTGC AGCAGCTCCG AGACAGTGGT GAGAAGGTGC TGGAGATTGT GAAGAAACTG 
GAGTCTCGCC AGCGCGGCTG GGAGGAGGAG GAGGACCCTG AGAGGAAGGG GACCATCGTG 
TTCAATGCCA CTTCTGAATT CTGCCGCACG CTGGGGGAGA TCCCCACCTA CGGGTTGGCT 
GGCAACCGCG AGGAGCAGGA AGAGCTCATG GACTTTGAAA GGGATGAGGA GCGCTCGGCC 
AACGGTGGCT CTGAATCAGA TGGGGAAGAG AACATTGGCT GGAGCACCGT CAACCTGGAT 
GAGGAGAAGC AGCATCAGGA TTTCTCTGCT TCCTCTACCA CCATCCTGGA TGAGGAGCCC 
ATCGTGAACA GAGGGCTGGC TGCTGCCCTG CTCCTGTGTC AGAACAAAGG ACTGCTGGAG 
ACCACAGTAC AAAAGGTGGC CCGGGTGAAG GCTCCCAATA AGTCACTGCC CTCAGCAGTG 
TACTGCATCG AGGATAAGAT GGCCATTGAT GACAAGTACA GCCGACGGGA GGAGTACAGG 
GGCTTCACCC AGGACTTCAA GGAGAAAGAT GGCTACAAGC CTGATGTTAA GATTGAATAC 
GTGGATGAGA CGGGCAGGAA ACTGACTCCC AAGGAGGCAT TCCGGCAGCT GTCCCACCGC 
TTCCACGGAA AGGGCTCAGG CAAGATGAAG ACTGAGCGGC GGATGAAGAA GCTGGATGAG 
GAGGCGCTCC TGAAGAAAAT GAGCTCCAGT GACACGCCCC TGGGCACCGT GGCACTGCTC 
CAGGAGAAGC AGAAGGCCCA GAAGACACCG TATATCGTGC TCAGTGGCAG TGGCAAGAGC 
ATGAATGCGA ACACCATCAC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106658.1
CDS	567..2513
Translation	MALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVLQDGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIRTKLRLQAQSLSSVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEKEVIMRADDLLPLGDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENMDISDEEDGGALPPGSPEGLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKLESRQRGWEEEEDPERKGTIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQHQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	XM_030250798.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus squamous cell carcinoma antigen recognized by T cells 1 (Sart1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250798.1

ORF Insert Sequence:

ATGGCCTTGC GACAGCGGGA AGAACTGCGG GAGAAGCTGG CAGCTGCCAA GGAAAAACGC 
TTGCTGAACC AAAAATTGGG GAAAATAAAG ACACTGGGGG AGGATGACCC CTGGCTGGAT 
GATACTGCAG CCTGGATAGA GAGGAGCCGG CAGCTACAGA AAGAGAAGGA CCTGGCAGAG 
AAGCGGGCTA AGCTACTAGA GGAGATGGAC CAAGAGTTTG GTGTGAGCAC TTTAGTGGAG 
GAGGAGTTTG AGCAGAGGCG ACAGGACCTG TACAGTGCCC GGGACCTGCA AGGCCTCACT 
GTGGAACATG CCATCGATTC TTTTCGAGAA GGGGAGACTG TGGTCCTCAC CCTCAAGGAC 
AAAGGGGTAC TGCAGGACGG GGAGGACGTG CTGGTGAATG TGAACATGGT GGACAAGGAG 
CGGGCGGACA AGAACGTGGA GCTTCGGAAA AAGAAGCCTG ACTACCTGCC CTATGCGGAA 
GATGAGAGTG TGGACGATTT GGCACAGCAA AAACCTCGTT CTATCCTGGC CAAATATGAT 
GAGGAACTGG AGGGCGAGCG ACCACATTCC TTCCGTCTGG AGCAGGGTGG GATGGCCGAT 
GGCCTGAGGG AACGAGAGTT AGAAGAAATC CGCACCAAGC TACGGCTGCA GGCTCAGTCT 
CTGAGCTCCG TAGGGCCCCG GCTTGCCTCT GAGTACCTCA GTCCCGAGGA GATGGTGACC 
TTCAAGAAGA CCAAACGGAG AGTGAAGAAA ATCCGAAAGA AGGAGAAGGA GGTGATAATG 
CGGGCAGATG ACTTGCTGCC CCTGGGCGAC CAGACTCAGG ATGGGGACTT TGGATCCAGG 
CTCCGGGGCC GGGGTCGGCG CCGAGTTCCT GAGGTGGAGG AGGAGGCCCT TGAGGATGAG 
GAGAAGGACC CTGTGGCCCA GCCGCCACCA TCGGATGACA CTCGAGTAGA GAACATGGAC 
ATCAGTGATG AAGAGGATGG GGGAGCTCTT CCGCCAGGGT CCCCAGAGGG GCTGGAGGAA 
GACGAGGCAG AGCTGGAGCT GCAGAAGCAG CTGGAGAAGG GGCGCCGGCT GCGGCAGCTG 
CAGCAACTGC AGCAGCTCCG AGACAGTGGT GAGAAGGTGC TGGAGATTGT GAAGAAACTG 
GAGTCTCGCC AGCGCGGCTG GGAGGAGGAG GAGGACCCTG AGAGGAAGGG GACCATCGTG 
TTCAATGCCA CTTCTGAATT CTGCCGCACG CTGGGGGAGA TCCCCACCTA CGGGTTGGCT 
GGCAACCGCG AGGAGCAGGA AGAGCTCATG GACTTTGAAA GGGATGAGGA GCGCTCGGCC 
AACGGTGGCT CTGAATCAGA TGGGGAAGAG AACATTGGCT GGAGCACCGT CAACCTGGAT 
GAGGAGAAGC AGCATCAGGA TTTCTCTGCT TCCTCTACCA CCATCCTGGA TGAGGAGCCC 
ATCGTGAACA GAGGGCTGGC TGCTGCCCTG CTCCTGTGTC AGAACAAAGG ACTGCTGGAG 
ACCACAGTAC AAAAGGTGGC CCGGGTGAAG GCTCCCAATA AGTCACTGCC CTCAGCAGTG 
TACTGCATCG AGGATAAGAT GGCCATTGAT GACAAGTACA GCCGACGGGA GGAGTACAGG 
GGCTTCACCC AGGACTTCAA GGAGAAAGAT GGCTACAAGC CTGATGTTAA GATTGAATAC 
GTGGATGAGA CGGGCAGGAA ACTGACTCCC AAGGAGGCAT TCCGGCAGCT GTCCCACCGC 
TTCCACGGAA AGGGCTCAGG CAAGATGAAG ACTGAGCGGC GGATGAAGAA GCTGGATGAG 
GAGGCGCTCC TGAAGAAAAT GAGCTCCAGT GACACGCCCC TGGGCACCGT GGCACTGCTC 
CAGGAGAAGC AGAAGGCCCA GAAGACACCG TATATCGTGC TCAGTGGCAG TGGCAAGAGC 
ATGAATGCGA ACACCATCAC CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new HIF-1α/RANTES-driven pathway to hepatocellular carcinoma mediated by germline haploinsufficiency of SART1/HAF in mice.
Hepatology (Baltimore, Md.)63(5)1576-91(2016 May)
Koh MY,Gagea M,Sargis T,Lemos R Jr,Grandjean G,Charbono A,Bekiaris V,Sedy J,Kiriakova G,Liu X,Roberts LR,Ware C,Powis G

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Epitope tagging of proteins at the native chromosomal loci of genes in mice and in cultured vertebrate cells.
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Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis.
Gene271(1)43-9(2001 Jun)
Bolland DJ,Hewitt JE

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