Nelfe cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfe
Entrez Gene ID	294258
Full Name	negative elongation factor complex member E
Synonyms	Rd,Rdbp
General protein information	Preferred Names negative elongation factor complex member E Names negative elongation factor E RD RNA-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_212548.2	NP_997713.1	negative elongation factor E
NM_001165901.1	NP_001159373.1	negative elongation factor E
XM_006255921.2	XP_006255983.1	negative elongation factor E isoform X1
XM_006255922.3	XP_006255984.1	negative elongation factor E isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RDBP	XP_003311227.1
Danio rerio (zebrafish)	nelfe	NP_001002375.1
Rattus norvegicus (Norway rat)	Nelfe	NP_001159373.1
Gallus gallus (chicken)	RDBP	XP_003642781.2

Gene Ontology
Bibliography

Related articles in PubMed

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.
Bastide MF, Bido S, Duteil N, Bézard E
Neurobiology of disease8593-98(2016 Jan)

Control of transcriptional elongation.
Kwak H, Lis JT
Annual review of genetics47483-508(2013)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Flynn RA, Almada AE, Zamudio JR, Sharp PA
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)

Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II.
Saha RN, Wissink EM, Bailey ER, Zhao M, Fargo DC, Hwang JY, Daigle KR, Fenn JD, Adelman K, Dudek SM
Nature neuroscience14(7)848-56(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nelfe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04888	NM_001165901.1 Latest version!	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa04888	NM_212548.2 Latest version!	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa04888	XM_006255922.3 Latest version!	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa39119	XM_006255921.2 Latest version!	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfe ( NM_212548.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997713.1 Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa04888
Clone ID Related Accession (Same CDS sequence)	NM_212548.2 , NM_001165901.1 , XM_006255922.3
Accession Version	NM_212548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC079427.1. On Jun 10, 2007 this sequence version replaced NM_212548.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC079427.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997713.1
CDS	110..1219
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGTASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGEGPPRGFDWSYEEHSGARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRERDKDKDRDRDRDRDKERDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL

Target ORF information:

RefSeq Version	NM_212548.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212548.2

ORF Insert Sequence:

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfe ( NM_001165901.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001159373.1 Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa04888
Clone ID Related Accession (Same CDS sequence)	NM_212548.2 , NM_001165901.1 , XM_006255922.3
Accession Version	NM_001165901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CO401737.1 and BC079427.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## CDS exon combination :: BC079427.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159373.1
CDS	78..1187
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGTASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGEGPPRGFDWSYEEHSGARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRERDKDKDRDRDRDRDKERDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL

Target ORF information:

RefSeq Version	NM_001165901.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165901.1

ORF Insert Sequence:

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfe ( XM_006255922.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255984.1 Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04888
Clone ID Related Accession (Same CDS sequence)	NM_212548.2 , NM_001165901.1 , XM_006255922.3
Accession Version	XM_006255922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255984.1
CDS	283..1392
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGTASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGEGPPRGFDWSYEEHSGARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRERDKDKDRDRDRDRDKERDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL

Target ORF information:

RefSeq Version	XM_006255922.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255922.3

ORF Insert Sequence:

ATGTTGGTGA TACCTCCGGG GCTGAGTGAA GAGGAAGAGG CACTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCGTTGCTG GCTCTGAAGA AGCAAAGCAG CAGTGGTACA 
GCGAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA TACTGCCACA 
GCAACAGAAC AGGCCAAACA GCTGGTGAAG TCAGGAGCTA TCAGTGCCAT CAAGGCTGAA 
ACTAAGAACT CAGGCTTCAA ACGTTCTCGG ACCCTGGAAG GGAAGTTAAA GGACCCAGAG 
AAGGGGCCTG TCCCCACCTT CCAGCCGTTC CAGAGGAGCA TGTCTGCTGA CGAGGACCTA 
CAGGAGCCAT CCAGACGGCC CCAAAGGAAA TCTCTGTATG AAAGCTTTGT GTCTTCTAGT 
GATCGGCTTC GGGAACTGGG ACAAGATGGA GAAGAAGCAG AGGCCCCTGG GGCTGGTGAA 
GGCCCACCTC GAGGTTTTGA CTGGAGCTAT GAAGAACACA GCGGTGCCCG ATCCTCAGCC 
TCCCCTCCCC GAAGCCGCAG CCGGGACAGA AGTCATGATC GGAGCCGAGA TAGGGACCGG 
GACAAAGAAC GAGACAGAGA CCGGGATCGA GACAGAGAAC GGGACAAAGA CAAAGACAGA 
GACCGGGACC GGGACAGAGA CAAAGAGCGG GACAGAGACA GGGACCGGGA GCGAGACCGA 
GAGGGCCCTT TCCGTAGGTC AGACTCATTC CCCGAACGGA GGGCCCCTCG AAAGGGGAAC 
ACTCTGTATG TGTATGGAGA GGACATGACG CCCACCCTCC TCCGAGGGGC CTTCTCTCCC 
TTTGGAAATA TCATCGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAC 
GAGAAGATGG AGTCAGCAGA TCAGGCTGTT GCTGAGCTCA ATGGGACCCA GGTGGAATCT 
GTGCAGCTCA AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGACGCTGC TACTGGCAAA 
TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC AGCCCCAAGG GTTGCCACCG GGACAAGAGG 
ACCCAGATTG TGTACAGTGA CGATCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfe ( XM_006255921.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255983.1 Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39119
Clone ID Related Accession (Same CDS sequence)	XM_006255921.2
Accession Version	XM_006255921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	negative elongation factor E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255921.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAG TAGAGAATGA TGTCTTGGGT TCTGCCCTTG AACCAGGAGC CAACATGTTG 
GTGATACCTC CGGGGCTGAG TGAAGAGGAA GAGGCACTAC AGAAGAAATT CAACAAACTC 
AAGAAAAAGA AAAAGGCGTT GCTGGCTCTG AAGAAGCAAA GCAGCAGTGG TACAGCGAGC 
CAGGGCGGTG TCAAGCGCTC ACTGTCAGAG CAGCCTGTGG TGGATACTGC CACAGCAACA 
GAACAGGCCA AACAGCTGGT GAAGTCAGGA GCTATCAGTG CCATCAAGGC TGAAACTAAG 
AACTCAGGCT TCAAACGTTC TCGGACCCTG GAAGGGAAGT TAAAGGACCC AGAGAAGGGG 
CCTGTCCCCA CCTTCCAGCC GTTCCAGAGG AGCATGTCTG CTGACGAGGA CCTACAGGAG 
CCATCCAGAC GGCCCCAAAG GAAATCTCTG TATGAAAGCT TTGTGTCTTC TAGTGATCGG 
CTTCGGGAAC TGGGACAAGA TGGAGAAGAA GCAGAGGCCC CTGGGGCTGG TGAAGGCCCA 
CCTCGAGGTT TTGACTGGAG CTATGAAGAA CACAGCGGTG CCCGATCCTC AGCCTCCCCT 
CCCCGAAGCC GCAGCCGGGA CAGAAGTCAT GATCGGAGCC GAGATAGGGA CCGGGACAAA 
GAACGAGACA GAGACCGGGA TCGAGACAGA GAACGGGACA AAGACAAAGA CAGAGACCGG 
GACCGGGACA GAGACAAAGA GCGGGACAGA GACAGGGACC GGGAGCGAGA CCGAGAGGGC 
CCTTTCCGTA GGTCAGACTC ATTCCCCGAA CGGAGGGCCC CTCGAAAGGG GAACACTCTG 
TATGTGTATG GAGAGGACAT GACGCCCACC CTCCTCCGAG GGGCCTTCTC TCCCTTTGGA 
AATATCATCG ACCTCTCCAT GGACCCACCC AGAAACTGTG CCTTCGTCAC CTACGAGAAG 
ATGGAGTCAG CAGATCAGGC TGTTGCTGAG CTCAATGGGA CCCAGGTGGA ATCTGTGCAG 
CTCAAAGTCA ACATAGCCCG AAAACAGCCC ATGCTGGACG CTGCTACTGG CAAATCTGTC 
TGGGGCTCCC TTGCTGTCCA GAACAGCCCC AAGGGTTGCC ACCGGGACAA GAGGACCCAG 
ATTGTGTACA GTGACGATCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255983.1
CDS	185..1348
Translation	MVTVENDVLGSALEPGANMLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSGTASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSMSADEDLQEPSRRPQRKSLYESFVSSSDRLRELGQDGEEAEAPGAGEGPPRGFDWSYEEHSGARSSASPPRSRSRDRSHDRSRDRDRDKERDRDRDRDRERDKDKDRDRDRDRDKERDRDRDRERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL

Target ORF information:

RefSeq Version	XM_006255921.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus negative elongation factor complex member E (Nelfe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255921.2

ORF Insert Sequence:

ATGGTGACAG TAGAGAATGA TGTCTTGGGT TCTGCCCTTG AACCAGGAGC CAACATGTTG 
GTGATACCTC CGGGGCTGAG TGAAGAGGAA GAGGCACTAC AGAAGAAATT CAACAAACTC 
AAGAAAAAGA AAAAGGCGTT GCTGGCTCTG AAGAAGCAAA GCAGCAGTGG TACAGCGAGC 
CAGGGCGGTG TCAAGCGCTC ACTGTCAGAG CAGCCTGTGG TGGATACTGC CACAGCAACA 
GAACAGGCCA AACAGCTGGT GAAGTCAGGA GCTATCAGTG CCATCAAGGC TGAAACTAAG 
AACTCAGGCT TCAAACGTTC TCGGACCCTG GAAGGGAAGT TAAAGGACCC AGAGAAGGGG 
CCTGTCCCCA CCTTCCAGCC GTTCCAGAGG AGCATGTCTG CTGACGAGGA CCTACAGGAG 
CCATCCAGAC GGCCCCAAAG GAAATCTCTG TATGAAAGCT TTGTGTCTTC TAGTGATCGG 
CTTCGGGAAC TGGGACAAGA TGGAGAAGAA GCAGAGGCCC CTGGGGCTGG TGAAGGCCCA 
CCTCGAGGTT TTGACTGGAG CTATGAAGAA CACAGCGGTG CCCGATCCTC AGCCTCCCCT 
CCCCGAAGCC GCAGCCGGGA CAGAAGTCAT GATCGGAGCC GAGATAGGGA CCGGGACAAA 
GAACGAGACA GAGACCGGGA TCGAGACAGA GAACGGGACA AAGACAAAGA CAGAGACCGG 
GACCGGGACA GAGACAAAGA GCGGGACAGA GACAGGGACC GGGAGCGAGA CCGAGAGGGC 
CCTTTCCGTA GGTCAGACTC ATTCCCCGAA CGGAGGGCCC CTCGAAAGGG GAACACTCTG 
TATGTGTATG GAGAGGACAT GACGCCCACC CTCCTCCGAG GGGCCTTCTC TCCCTTTGGA 
AATATCATCG ACCTCTCCAT GGACCCACCC AGAAACTGTG CCTTCGTCAC CTACGAGAAG 
ATGGAGTCAG CAGATCAGGC TGTTGCTGAG CTCAATGGGA CCCAGGTGGA ATCTGTGCAG 
CTCAAAGTCA ACATAGCCCG AAAACAGCCC ATGCTGGACG CTGCTACTGG CAAATCTGTC 
TGGGGCTCCC TTGCTGTCCA GAACAGCCCC AAGGGTTGCC ACCGGGACAA GAGGACCCAG 
ATTGTGTACA GTGACGATCT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.
Neurobiology of disease8593-98(2016 Jan)
Bastide MF,Bido S,Duteil N,Bézard E

Control of transcriptional elongation.
Annual review of genetics47483-508(2013)
Kwak H,Lis JT

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)
Flynn RA,Almada AE,Zamudio JR,Sharp PA

Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II.
Nature neuroscience14(7)848-56(2011 May)
Saha RN,Wissink EM,Bailey ER,Zhao M,Fargo DC,Hwang JY,Daigle KR,Fenn JD,Adelman K,Dudek SM

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Nelfe cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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