Ganab cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ganab
Entrez Gene ID	293721
Full Name	glucosidase II alpha subunit
Synonyms	G2an
General protein information	Preferred Names glucosidase II alpha subunit Names neutral alpha-glucosidase AB alpha glucosidase 2 alpha neutral subunit glucosidase, alpha glucosidase, alpha; neutral AB neutral AB
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230976.3	XP_006231038.1	neutral alpha-glucosidase AB isoform X1
NM_001106334.1	NP_001099804.1	neutral alpha-glucosidase AB
XM_006230977.3	XP_006231039.1	neutral alpha-glucosidase AB isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GANAB	NP_938149.2
Bos taurus (cattle)	GANAB	NP_001192706.1
Drosophila melanogaster (fruit fly)	CG14476	NP_652145.1
Caenorhabditis elegans (roundworm)	aagr-4	NP_508105.2
Saccharomyces cerevisiae (baker's yeast)	ROT2	NP_009788.3
Neurospora crassa	NCU04203	XP_961163.2
Xenopus tropicalis (tropical clawed frog)	ganab	XP_004919758.1
Oryza sativa (rice)	Os03g0216600	NP_001049385.1
Canis lupus familiaris (dog)	GANAB	XP_540905.2
Mus musculus (house mouse)	Ganab	NP_032086.1
Danio rerio (zebrafish)	LOC100334924	XP_002664508.2
Pan troglodytes (chimpanzee)	GANAB	XP_508494.2
Caenorhabditis elegans (roundworm)	aagr-3	NP_505507.1
Eremothecium gossypii	AGOS_AAR173C	NP_982716.1
Kluyveromyces lactis	KLLA0F09735g	XP_455522.1
Macaca mulatta (Rhesus monkey)	GANAB	XP_001116396.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000862	XP_316832.4
Schizosaccharomyces pombe (fission yeast)	gls2	NP_593490.1
Magnaporthe oryzae (rice blast fungus)	MGG_08623	XP_003711051.1
Rattus norvegicus (Norway rat)	Ganab	NP_001099804.1
Arabidopsis thaliana (thale cress)	RSW3	NP_201189.1
Danio rerio (zebrafish)	ganab	XP_002664506.1

Gene Ontology
Bibliography

Related articles in PubMed

Phenotypically Silent Bone Morphogenetic Protein Receptor 2 Mutations Predispose Rats to Inflammation-Induced Pulmonary Arterial Hypertension by Enhancing the Risk for Neointimal Transformation.
Tian W, Jiang X, Sung YK, Shuffle E, Wu TH, Kao PN, Tu AB, Dorfmüller P, Cao A, Wang L, Peng G, Kim Y, Zhang P, Chappell J, Pasupneti S, Dahms P, Maguire P, Chaib H, Zamanian R, Peters-Golden M, Snyder MP, Voelkel NF, Humbert M, Rabinovitch M, Nicolls MR
Circulation140(17)1409-1425(2019 Oct)

Isolated polycystic liver disease genes define effectors of polycystin-1 function.
Besse W, Dong K, Choi J, Punia S, Fedeles SV, Choi M, Gallagher AR, Huang EB, Gulati A, Knight J, Mane S, Tahvanainen E, Tahvanainen P, Sanna-Cherchi S, Lifton RP, Watnick T, Pei YP, Torres VE, Somlo S
The Journal of clinical investigation127(5)1772-1785(2017 May)

Structures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB, Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P, Zitzmann N
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)

Mutations in GANAB, Encoding the Glucosidase IIα Subunit, Cause Autosomal-Dominant Polycystic Kidney and Liver Disease.
Porath B, Gainullin VG, Cornec-Le Gall E, Dillinger EK, Heyer CM, Hopp K, Edwards ME, Madsen CD, Mauritz SR, Banks CJ, Baheti S, Reddy B, Herrero JI, Bañales JM, Hogan MC, Tasic V, Watnick TJ, Chapman AB, Vigneau C, Lavainne F, Audrézet MP, Ferec C, Le Meur Y, Torres VE, , , Harris PC
American journal of human genetics98(6)1193-1207(2016 06)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hernández-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ganab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ganab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04638	NM_001106334.1 Latest version!	Rattus norvegicus glucosidase II alpha subunit (Ganab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa23870	XM_006230976.3 Latest version!	Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa23871	XM_006230977.3 Latest version!	Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ganab ( NM_001106334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099804.1 Rattus norvegicus glucosidase II alpha subunit (Ganab), mRNA.

Price & Availability↑

CloneID	ORa04638
Clone ID Related Accession (Same CDS sequence)	NM_001106334.1
Accession Version	NM_001106334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571673600000
Organism	Rattus norvegicus(Norway rat)
Product	neutral alpha-glucosidase AB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473953.1. On or before Oct 4, 2007 this sequence version replaced XM_215144.4, XM_001077792.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTGG ACTTATGGCT TTTGAGCATC AGAGGGCTCC CAGGGTCCCT TTCTCGGATA 
AAGTTAGTCT CACGCTCGGT AGCATGTGGG ATAAGATCAA GAACCTTTTC TCTAGGCAAG 
AACCAAAAGA TGCAGCTGAA GGCAACGGAG CCGAGCCTGA AGCAAGGCCT GGGGATGGTG 
ACAAGGCAAC CAGAGGAGAC CCAGGAAAAG GCTGAGAAAG ATGAGCCAGG AGCCTGGGAG 
GAGACATTCA AAACACACTC TGACAGCAAG CCTTACGGCC CCACGTCGGT AGGTTTGGAC 
TTTTCTCTGC CAGGAATGGA ACATGTGTAT GGGATCCCTG AGCATGCAGA CAGCCTGAGA 
CTGAAGGTCA CTGAGGGTGG TGAGCCGTAC CGTCTGTACA ACTTGGATGT GTTCCAGTAT 
GAGCTGAACA ACCCCATGGC TTTGTATGGG TCTGTGCCTG TGCTCTTGGC ACACAGCTTT 
CATCGGGACT TGGGTATCTT CTGGCTTAAT GCTGCTGAGA CATGGGTTGA TATATCGTCC 
AACACAGCTG GGAAGACCCT GTTTGGGAAG ATGCTGGATT ACCTGCAGGG GTCTGGGGAG 
ACTCCACAGA CAGACATTCG TTGGATGTCC GAGAGCGGCA TTGTTGATGT TTTCCTCATG 
CTCGGCCCTT CAGTCCTTGA TGTCTTCCGG CAGCATGCTA GCCTTACAGG GACCCAGGCA 
CTACCCCCAC TCTTCTCCCT GGGCTACCAC CAGAGTCGCT GGAACTACCG GGATGAGGCT 
GATGTTTTGG AAGTGGATCA GGGTTTTGAT GATCACAACA TGCCTTGTGA TGTGATATGG 
TTGGACATTG AACATGCTGA TGGCAAGCGG TACTTCACTT GGGACCCCAC CCGATTTCCT 
CAGCCCCTCA ATATGCTTGA GCACTTGGCT TCTAAAAGGC GGAAGCTGGT GGCCATCGTG 
GATCCCCACA TCAAGGTAGA CTCTGGTTAC CGAGTTCATG AAGAATTGCG AAACCATGGG 
CTGTATGTTA AAACTCGGGA TGGCTCTGAT TACGAGGGCT GGTGCTGGCC AGGTTCGGCT 
AGTTACCCTG ACTTCACTAA CCCACAGATG AGGGCCTGGT GGGCTAACAT GTTCAACTTT 
CACAACTATG AGGGTTCAGC TCCTAATCTT TTTGTCTGGA ATGACATGAA TGAACCGTCT 
GTGTTCAATG GTCCTGAAGT CACCATGCTG AAGGATGCTG TGCATTATGG CGGCTGGGAG 
CACCGGGACA TCCATAACAT CTATGGCTTA TATGTGCACA TGGCAACTGC TGACGGGCTA 
ATACAGCGCT CTGGTGGCAT AGAGCGGCCC TTTGTCCTGA GTAGGGCCTT CTTCTCAGGC 
TCCCAGCGCT TTGGAGCTGT GTGGACAGGG GACAACACTG CAGAGTGGGA TCATTTGAAG 
ATCTCTATCC CTATGTGTCT CAGCCTGGCA CTGGTGGGGC TTTCCTTCTG TGGCGCGGAT 
GTGGGTGGCT TCTTCAAGAA CCCAGAGCCA GAACTGCTTG TGCGCTGGTA CCAAATGGGT 
GCCTACCAGC CATTCTTTCG GGCTCATGCT CACTTGGACA CTGGGCGGCG AGAGCCGTGG 
CTGTTAGCGT CTCAATACCA AGATGCAATC CGAGATGCCT TGTTCCAGCG ATACTCTTTG 
CTGCCCTTCT GGTATACCCT CTTCTATCAA GCTCACCAGG AAGGGTTTCC TGTCATGAGG 
CCCCTCTGGG TACAGTATCC TGAGGATATG TCTACCTTCA GTATGGAGGA TCAGTTCATG 
CTTGGGGACG CACTCCTTAT TCACCCTGTG TCGGATGCTG GGGCCCATGG AGTGCAGGTC 
TATTTGCCTG GCGAAGAAGA GGTGTGGTAT GACATTCAAA GCTATCAGAA GCATCATGGT 
CCCCAGACCT TGTATCTGCC AGTGACCCTG AGCAGTATAC CTGTGTTCCA GCGTGGCGGG 
ACAATTGTCC CTCGATGGAT GCGCGTGAGG CGTTCTTCAG ACTGTATGAA GGAAGATCCT 
ATCACTCTCT TTGTTGCTCT CAGTGCCCAG GGCACGGCCC AAGGAGAGCT CTTTCTCGAT 
GATGGACACA CTTTTAACTA TCAGAATCGC CATGAGTTCC TGTTGCGTCG ATTCTCTTTC 
TCTGGCAACA CACTTGTCTC TAGTTCAGCA GACCCCAAAG GCCACCTTGA GACACCTGTC 
TGGATTGAGC GAGTGGTCAT AATGGGGGCT GGAAAGCCAG CAGCCGTGGT GCTCCAGACC 
AAAGGCTCTC CTGAAAGTCG CCTGTCCTTC CAGCATGACC CTGAGACCTC TGTGCTGACA 
TTGCGTAAAC CTGGCGTCAA TGTGGCATCT GACTGGAGTA TTCATCTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099804.1
CDS	541..2934
Translation	MPVDLWLLSIRGLPGSLSRIKLVSRSVACGIRSRTFSLGKNQKMQLKATEPSLKQGLGMVTRQPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVYGIPEHADSLRLKVTEGGEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRDLGIFWLNAAETWVDISSNTAGKTLFGKMLDYLQGSGETPQTDIRWMSESGIVDVFLMLGPSVLDVFRQHASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYVKTRDGSDYEGWCWPGSASYPDFTNPQMRAWWANMFNFHNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHQEGFPVMRPLWVQYPEDMSTFSMEDQFMLGDALLIHPVSDAGAHGVQVYLPGEEEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKEDPITLFVALSAQGTAQGELFLDDGHTFNYQNRHEFLLRRFSFSGNTLVSSSADPKGHLETPVWIERVVIMGAGKPAAVVLQTKGSPESRLSFQHDPETSVLTLRKPGVNVASDWSIHLR

Target ORF information:

RefSeq Version	NM_001106334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosidase II alpha subunit (Ganab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106334.1

ORF Insert Sequence:

ATGCCCGTGG ACTTATGGCT TTTGAGCATC AGAGGGCTCC CAGGGTCCCT TTCTCGGATA 
AAGTTAGTCT CACGCTCGGT AGCATGTGGG ATAAGATCAA GAACCTTTTC TCTAGGCAAG 
AACCAAAAGA TGCAGCTGAA GGCAACGGAG CCGAGCCTGA AGCAAGGCCT GGGGATGGTG 
ACAAGGCAAC CAGAGGAGAC CCAGGAAAAG GCTGAGAAAG ATGAGCCAGG AGCCTGGGAG 
GAGACATTCA AAACACACTC TGACAGCAAG CCTTACGGCC CCACGTCGGT AGGTTTGGAC 
TTTTCTCTGC CAGGAATGGA ACATGTGTAT GGGATCCCTG AGCATGCAGA CAGCCTGAGA 
CTGAAGGTCA CTGAGGGTGG TGAGCCGTAC CGTCTGTACA ACTTGGATGT GTTCCAGTAT 
GAGCTGAACA ACCCCATGGC TTTGTATGGG TCTGTGCCTG TGCTCTTGGC ACACAGCTTT 
CATCGGGACT TGGGTATCTT CTGGCTTAAT GCTGCTGAGA CATGGGTTGA TATATCGTCC 
AACACAGCTG GGAAGACCCT GTTTGGGAAG ATGCTGGATT ACCTGCAGGG GTCTGGGGAG 
ACTCCACAGA CAGACATTCG TTGGATGTCC GAGAGCGGCA TTGTTGATGT TTTCCTCATG 
CTCGGCCCTT CAGTCCTTGA TGTCTTCCGG CAGCATGCTA GCCTTACAGG GACCCAGGCA 
CTACCCCCAC TCTTCTCCCT GGGCTACCAC CAGAGTCGCT GGAACTACCG GGATGAGGCT 
GATGTTTTGG AAGTGGATCA GGGTTTTGAT GATCACAACA TGCCTTGTGA TGTGATATGG 
TTGGACATTG AACATGCTGA TGGCAAGCGG TACTTCACTT GGGACCCCAC CCGATTTCCT 
CAGCCCCTCA ATATGCTTGA GCACTTGGCT TCTAAAAGGC GGAAGCTGGT GGCCATCGTG 
GATCCCCACA TCAAGGTAGA CTCTGGTTAC CGAGTTCATG AAGAATTGCG AAACCATGGG 
CTGTATGTTA AAACTCGGGA TGGCTCTGAT TACGAGGGCT GGTGCTGGCC AGGTTCGGCT 
AGTTACCCTG ACTTCACTAA CCCACAGATG AGGGCCTGGT GGGCTAACAT GTTCAACTTT 
CACAACTATG AGGGTTCAGC TCCTAATCTT TTTGTCTGGA ATGACATGAA TGAACCGTCT 
GTGTTCAATG GTCCTGAAGT CACCATGCTG AAGGATGCTG TGCATTATGG CGGCTGGGAG 
CACCGGGACA TCCATAACAT CTATGGCTTA TATGTGCACA TGGCAACTGC TGACGGGCTA 
ATACAGCGCT CTGGTGGCAT AGAGCGGCCC TTTGTCCTGA GTAGGGCCTT CTTCTCAGGC 
TCCCAGCGCT TTGGAGCTGT GTGGACAGGG GACAACACTG CAGAGTGGGA TCATTTGAAG 
ATCTCTATCC CTATGTGTCT CAGCCTGGCA CTGGTGGGGC TTTCCTTCTG TGGCGCGGAT 
GTGGGTGGCT TCTTCAAGAA CCCAGAGCCA GAACTGCTTG TGCGCTGGTA CCAAATGGGT 
GCCTACCAGC CATTCTTTCG GGCTCATGCT CACTTGGACA CTGGGCGGCG AGAGCCGTGG 
CTGTTAGCGT CTCAATACCA AGATGCAATC CGAGATGCCT TGTTCCAGCG ATACTCTTTG 
CTGCCCTTCT GGTATACCCT CTTCTATCAA GCTCACCAGG AAGGGTTTCC TGTCATGAGG 
CCCCTCTGGG TACAGTATCC TGAGGATATG TCTACCTTCA GTATGGAGGA TCAGTTCATG 
CTTGGGGACG CACTCCTTAT TCACCCTGTG TCGGATGCTG GGGCCCATGG AGTGCAGGTC 
TATTTGCCTG GCGAAGAAGA GGTGTGGTAT GACATTCAAA GCTATCAGAA GCATCATGGT 
CCCCAGACCT TGTATCTGCC AGTGACCCTG AGCAGTATAC CTGTGTTCCA GCGTGGCGGG 
ACAATTGTCC CTCGATGGAT GCGCGTGAGG CGTTCTTCAG ACTGTATGAA GGAAGATCCT 
ATCACTCTCT TTGTTGCTCT CAGTGCCCAG GGCACGGCCC AAGGAGAGCT CTTTCTCGAT 
GATGGACACA CTTTTAACTA TCAGAATCGC CATGAGTTCC TGTTGCGTCG ATTCTCTTTC 
TCTGGCAACA CACTTGTCTC TAGTTCAGCA GACCCCAAAG GCCACCTTGA GACACCTGTC 
TGGATTGAGC GAGTGGTCAT AATGGGGGCT GGAAAGCCAG CAGCCGTGGT GCTCCAGACC 
AAAGGCTCTC CTGAAAGTCG CCTGTCCTTC CAGCATGACC CTGAGACCTC TGTGCTGACA 
TTGCGTAAAC CTGGCGTCAA TGTGGCATCT GACTGGAGTA TTCATCTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ganab ( XM_006230976.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231038.1 Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23870
Clone ID Related Accession (Same CDS sequence)	XM_006230976.3
Accession Version	XM_006230976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	neutral alpha-glucosidase AB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TAGCGGCAGT GGCGGCGCGT AGGAGGCGGT CTTGGTTAAG TTTGGTGCTG 
GCATACTTAG GGGTCTGTCT GGGGATTACT CTTGCTGTGG ATAGAAGCAA CTTTAAGACC 
TGTGAAGAGA GTTCCTTTTG CAAACGGCAG CGAAGCATTC GGCCAGGCCT CTCTCCTTAC 
CGCGCCTTGC TGGACACTCT GCAGCTTGGT CCTGATGCTC TTACAGTCCA TCTGACCCAT 
GAAGTCACCA AGGTGTTGCT TGTGCTGGAG CTCCAGGGCC TTCAGAAGAA CATGACTCGG 
ATCAGGATCG ATGAGCTAGA GCCCCGGCGG CCTCGATACC GAGTGCCAGA TGTTTTAGTG 
GCTGACCCCG CCACAGCTCG GCTTTCGATT TCTGGCCGTG ATGACAACAG TGTGGAGCTA 
ACAGTGGCTG AAGGACCTTA CAAAATCATT TTGACAGCAA AGCCATTCCG CCTTGACCTG 
CTAGAAGACC GAAGCCTCCT GCTCAGTGTC AATGCCCGTG GACTTATGGC TTTTGAGCAT 
CAGAGGGCTC CCAGGGTCCC TTTCTCGGAT AAAGTTAGTC TCACGCTCGG TAGCATGTGG 
GATAAGATCA AGAACCTTTT CTCTAGGCAA GAACCAAAAG ATGCAGCTGA AGGCAACGGA 
GCCGAGCCTG AAGCAAGGCC TGGGGATGGT GACAAGCCAG AGGAGACCCA GGAAAAGGCT 
GAGAAAGATG AGCCAGGAGC CTGGGAGGAG ACATTCAAAA CACACTCTGA CAGCAAGCCT 
TACGGCCCCA CGTCGGTAGG TTTGGACTTT TCTCTGCCAG GAATGGAACA TGTGTATGGG 
ATCCCTGAGC ATGCAGACAG CCTGAGACTG AAGGTCACTG AGGGTGGTGA GCCGTACCGT 
CTGTACAACT TGGATGTGTT CCAGTATGAG CTGAACAACC CCATGGCTTT GTATGGGTCT 
GTGCCTGTGC TCTTGGCACA CAGCTTTCAT CGGGACTTGG GTATCTTCTG GCTTAATGCT 
GCTGAGACAT GGGTTGATAT ATCGTCCAAC ACAGCTGGGA AGACCCTGTT TGGGAAGATG 
CTGGATTACC TGCAGGGGTC TGGGGAGACT CCACAGACAG ACATTCGTTG GATGTCCGAG 
AGCGGCATTG TTGATGTTTT CCTCATGCTC GGCCCTTCAG TCCTTGATGT CTTCCGGCAG 
CATGCTAGCC TTACAGGGAC CCAGGCACTA CCCCCACTCT TCTCCCTGGG CTACCACCAG 
AGTCGCTGGA ACTACCGGGA TGAGGCTGAT GTTTTGGAAG TGGATCAGGG TTTTGATGAT 
CACAACATGC CTTGTGATGT GATATGGTTG GACATTGAAC ATGCTGATGG CAAGCGGTAC 
TTCACTTGGG ACCCCACCCG ATTTCCTCAG CCCCTCAATA TGCTTGAGCA CTTGGCTTCT 
AAAAGGCGGA AGCTGGTGGC CATCGTGGAT CCCCACATCA AGGTAGACTC TGGTTACCGA 
GTTCATGAAG AATTGCGAAA CCATGGGCTG TATGTTAAAA CTCGGGATGG CTCTGATTAC 
GAGGGCTGGT GCTGGCCAGG TTCGGCTAGT TACCCTGACT TCACTAACCC ACAGATGAGG 
GCCTGGTGGG CTAACATGTT CAACTTTCAC AACTATGAGG GTTCAGCTCC TAATCTTTTT 
GTCTGGAATG ACATGAATGA ACCGTCTGTG TTCAATGGTC CTGAAGTCAC CATGCTGAAG 
GATGCTGTGC ATTATGGCGG CTGGGAGCAC CGGGACATCC ATAACATCTA TGGCTTATAT 
GTGCACATGG CAACTGCTGA CGGGCTAATA CAGCGCTCTG GTGGCATAGA GCGGCCCTTT 
GTCCTGAGTA GGGCCTTCTT CTCAGGCTCC CAGCGCTTTG GAGCTGTGTG GACAGGGGAC 
AACACTGCAG AGTGGGATCA TTTGAAGATC TCTATCCCTA TGTGTCTCAG CCTGGCACTG 
GTGGGGCTTT CCTTCTGTGG CGCGGATGTG GGTGGCTTCT TCAAGAACCC AGAGCCAGAA 
CTGCTTGTGC GCTGGTACCA AATGGGTGCC TACCAGCCAT TCTTTCGGGC TCATGCTCAC 
TTGGACACTG GGCGGCGAGA GCCGTGGCTG TTAGCGTCTC AATACCAAGA TGCAATCCGA 
GATGCCTTGT TCCAGCGATA CTCTTTGCTG CCCTTCTGGT ATACCCTCTT CTATCAAGCT 
CACCAGGAAG GGTTTCCTGT CATGAGGCCC CTCTGGGTAC AGTATCCTGA GGATATGTCT 
ACCTTCAGTA TGGAGGATCA GTTCATGCTT GGGGACGCAC TCCTTATTCA CCCTGTGTCG 
GATGCTGGGG CCCATGGAGT GCAGGTCTAT TTGCCTGGCG AAGAAGAGGT GTGGTATGAC 
ATTCAAAGCT ATCAGAAGCA TCATGGTCCC CAGACCTTGT ATCTGCCAGT GACCCTGAGC 
AGTATACCTG TGTTCCAGCG TGGCGGGACA ATTGTCCCTC GATGGATGCG CGTGAGGCGT 
TCTTCAGACT GTATGAAGGA AGATCCTATC ACTCTCTTTG TTGCTCTCAG TGCCCAGGGC 
ACGGCCCAAG GAGAGCTCTT TCTCGATGAT GGACACACTT TTAACTATCA GAATCGCCAT 
GAGTTCCTGT TGCGTCGATT CTCTTTCTCT GGCAACACAC TTGTCTCTAG TTCAGCAGAC 
CCCAAAGGCC ACCTTGAGAC ACCTGTCTGG ATTGAGCGAG TGGTCATAAT GGGGGCTGGA 
AAGCCAGCAG CCGTGGTGCT CCAGACCAAA GGCTCTCCTG AAAGTCGCCT GTCCTTCCAG 
CATGACCCTG AGACCTCTGT GCTGACATTG CGTAAACCTG GCGTCAATGT GGCATCTGAC 
TGGAGTATTC ATCTTCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231038.1
CDS	70..2970
Translation	MAAIAAVAARRRRSWLSLVLAYLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDTLQLGPDALTVHLTHEVTKVLLVLELQGLQKNMTRIRIDELEPRRPRYRVPDVLVADPATARLSISGRDDNSVELTVAEGPYKIILTAKPFRLDLLEDRSLLLSVNARGLMAFEHQRAPRVPFSDKVSLTLGSMWDKIKNLFSRQEPKDAAEGNGAEPEARPGDGDKPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVYGIPEHADSLRLKVTEGGEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRDLGIFWLNAAETWVDISSNTAGKTLFGKMLDYLQGSGETPQTDIRWMSESGIVDVFLMLGPSVLDVFRQHASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYVKTRDGSDYEGWCWPGSASYPDFTNPQMRAWWANMFNFHNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHQEGFPVMRPLWVQYPEDMSTFSMEDQFMLGDALLIHPVSDAGAHGVQVYLPGEEEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKEDPITLFVALSAQGTAQGELFLDDGHTFNYQNRHEFLLRRFSFSGNTLVSSSADPKGHLETPVWIERVVIMGAGKPAAVVLQTKGSPESRLSFQHDPETSVLTLRKPGVNVASDWSIHLR

Target ORF information:

RefSeq Version	XM_006230976.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230976.3

ORF Insert Sequence:

ATGGCGGCGA TAGCGGCAGT GGCGGCGCGT AGGAGGCGGT CTTGGTTAAG TTTGGTGCTG 
GCATACTTAG GGGTCTGTCT GGGGATTACT CTTGCTGTGG ATAGAAGCAA CTTTAAGACC 
TGTGAAGAGA GTTCCTTTTG CAAACGGCAG CGAAGCATTC GGCCAGGCCT CTCTCCTTAC 
CGCGCCTTGC TGGACACTCT GCAGCTTGGT CCTGATGCTC TTACAGTCCA TCTGACCCAT 
GAAGTCACCA AGGTGTTGCT TGTGCTGGAG CTCCAGGGCC TTCAGAAGAA CATGACTCGG 
ATCAGGATCG ATGAGCTAGA GCCCCGGCGG CCTCGATACC GAGTGCCAGA TGTTTTAGTG 
GCTGACCCCG CCACAGCTCG GCTTTCGATT TCTGGCCGTG ATGACAACAG TGTGGAGCTA 
ACAGTGGCTG AAGGACCTTA CAAAATCATT TTGACAGCAA AGCCATTCCG CCTTGACCTG 
CTAGAAGACC GAAGCCTCCT GCTCAGTGTC AATGCCCGTG GACTTATGGC TTTTGAGCAT 
CAGAGGGCTC CCAGGGTCCC TTTCTCGGAT AAAGTTAGTC TCACGCTCGG TAGCATGTGG 
GATAAGATCA AGAACCTTTT CTCTAGGCAA GAACCAAAAG ATGCAGCTGA AGGCAACGGA 
GCCGAGCCTG AAGCAAGGCC TGGGGATGGT GACAAGCCAG AGGAGACCCA GGAAAAGGCT 
GAGAAAGATG AGCCAGGAGC CTGGGAGGAG ACATTCAAAA CACACTCTGA CAGCAAGCCT 
TACGGCCCCA CGTCGGTAGG TTTGGACTTT TCTCTGCCAG GAATGGAACA TGTGTATGGG 
ATCCCTGAGC ATGCAGACAG CCTGAGACTG AAGGTCACTG AGGGTGGTGA GCCGTACCGT 
CTGTACAACT TGGATGTGTT CCAGTATGAG CTGAACAACC CCATGGCTTT GTATGGGTCT 
GTGCCTGTGC TCTTGGCACA CAGCTTTCAT CGGGACTTGG GTATCTTCTG GCTTAATGCT 
GCTGAGACAT GGGTTGATAT ATCGTCCAAC ACAGCTGGGA AGACCCTGTT TGGGAAGATG 
CTGGATTACC TGCAGGGGTC TGGGGAGACT CCACAGACAG ACATTCGTTG GATGTCCGAG 
AGCGGCATTG TTGATGTTTT CCTCATGCTC GGCCCTTCAG TCCTTGATGT CTTCCGGCAG 
CATGCTAGCC TTACAGGGAC CCAGGCACTA CCCCCACTCT TCTCCCTGGG CTACCACCAG 
AGTCGCTGGA ACTACCGGGA TGAGGCTGAT GTTTTGGAAG TGGATCAGGG TTTTGATGAT 
CACAACATGC CTTGTGATGT GATATGGTTG GACATTGAAC ATGCTGATGG CAAGCGGTAC 
TTCACTTGGG ACCCCACCCG ATTTCCTCAG CCCCTCAATA TGCTTGAGCA CTTGGCTTCT 
AAAAGGCGGA AGCTGGTGGC CATCGTGGAT CCCCACATCA AGGTAGACTC TGGTTACCGA 
GTTCATGAAG AATTGCGAAA CCATGGGCTG TATGTTAAAA CTCGGGATGG CTCTGATTAC 
GAGGGCTGGT GCTGGCCAGG TTCGGCTAGT TACCCTGACT TCACTAACCC ACAGATGAGG 
GCCTGGTGGG CTAACATGTT CAACTTTCAC AACTATGAGG GTTCAGCTCC TAATCTTTTT 
GTCTGGAATG ACATGAATGA ACCGTCTGTG TTCAATGGTC CTGAAGTCAC CATGCTGAAG 
GATGCTGTGC ATTATGGCGG CTGGGAGCAC CGGGACATCC ATAACATCTA TGGCTTATAT 
GTGCACATGG CAACTGCTGA CGGGCTAATA CAGCGCTCTG GTGGCATAGA GCGGCCCTTT 
GTCCTGAGTA GGGCCTTCTT CTCAGGCTCC CAGCGCTTTG GAGCTGTGTG GACAGGGGAC 
AACACTGCAG AGTGGGATCA TTTGAAGATC TCTATCCCTA TGTGTCTCAG CCTGGCACTG 
GTGGGGCTTT CCTTCTGTGG CGCGGATGTG GGTGGCTTCT TCAAGAACCC AGAGCCAGAA 
CTGCTTGTGC GCTGGTACCA AATGGGTGCC TACCAGCCAT TCTTTCGGGC TCATGCTCAC 
TTGGACACTG GGCGGCGAGA GCCGTGGCTG TTAGCGTCTC AATACCAAGA TGCAATCCGA 
GATGCCTTGT TCCAGCGATA CTCTTTGCTG CCCTTCTGGT ATACCCTCTT CTATCAAGCT 
CACCAGGAAG GGTTTCCTGT CATGAGGCCC CTCTGGGTAC AGTATCCTGA GGATATGTCT 
ACCTTCAGTA TGGAGGATCA GTTCATGCTT GGGGACGCAC TCCTTATTCA CCCTGTGTCG 
GATGCTGGGG CCCATGGAGT GCAGGTCTAT TTGCCTGGCG AAGAAGAGGT GTGGTATGAC 
ATTCAAAGCT ATCAGAAGCA TCATGGTCCC CAGACCTTGT ATCTGCCAGT GACCCTGAGC 
AGTATACCTG TGTTCCAGCG TGGCGGGACA ATTGTCCCTC GATGGATGCG CGTGAGGCGT 
TCTTCAGACT GTATGAAGGA AGATCCTATC ACTCTCTTTG TTGCTCTCAG TGCCCAGGGC 
ACGGCCCAAG GAGAGCTCTT TCTCGATGAT GGACACACTT TTAACTATCA GAATCGCCAT 
GAGTTCCTGT TGCGTCGATT CTCTTTCTCT GGCAACACAC TTGTCTCTAG TTCAGCAGAC 
CCCAAAGGCC ACCTTGAGAC ACCTGTCTGG ATTGAGCGAG TGGTCATAAT GGGGGCTGGA 
AAGCCAGCAG CCGTGGTGCT CCAGACCAAA GGCTCTCCTG AAAGTCGCCT GTCCTTCCAG 
CATGACCCTG AGACCTCTGT GCTGACATTG CGTAAACCTG GCGTCAATGT GGCATCTGAC 
TGGAGTATTC ATCTTCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ganab ( XM_006230977.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231039.1 Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X2, mRNA.

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CloneID	ORa23871
Clone ID Related Accession (Same CDS sequence)	XM_006230977.3
Accession Version	XM_006230977.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	neutral alpha-glucosidase AB isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TAGCGGCAGT GGCGGCGCGT AGGAGGCGGT CTTGGTTAAG TTTGGTGCTG 
GCATACTTAG GGGTCTGTCT GGGGATTACT CTTGCTGTGG ATAGAAGCAA CTTTAAGACC 
TGTGAAGAGA GTTCCTTTTG CAAACGGCAG CGAAGCATTC GGCCAGGCCT CTCTCCTTAC 
CGCGCCTTGC TGGACACTCT GCAGCTTGGT CCTGATGCTC TTACAGTCCA TCTGACCCAT 
GAAGTCACCA AGGTGTTGCT TGTGCTGGAG CTCCAGGGCC TTCAGAAGAA CATGACTCGG 
ATCAGGATCG ATGAGCTAGA GCCCCGGCGG CCTCGATACC GAGTGCCAGA TGTTTTAGTG 
GCTGACCCCG CCACAGCTCG GCTTTCGATT TCTGGCCGTG ATGACAACAG TGTGGAGCTA 
ACAGTGGCTG AAGGACCTTA CAAAATCATT TTGACAGCAA AGCCATTCCG CCTTGACCTG 
CTAGAAGACC GAAGCCTCCT GCTCAGTGTC AATGCCCGTG GACTTATGGC TTTTGAGCAT 
CAGAGGGCTC CCAGGGTCCC GCAAGAACCA AAAGATGCAG CTGAAGGCAA CGGAGCCGAG 
CCTGAAGCAA GGCCTGGGGA TGGTGACAAG CCAGAGGAGA CCCAGGAAAA GGCTGAGAAA 
GATGAGCCAG GAGCCTGGGA GGAGACATTC AAAACACACT CTGACAGCAA GCCTTACGGC 
CCCACGTCGG TAGGTTTGGA CTTTTCTCTG CCAGGAATGG AACATGTGTA TGGGATCCCT 
GAGCATGCAG ACAGCCTGAG ACTGAAGGTC ACTGAGGGTG GTGAGCCGTA CCGTCTGTAC 
AACTTGGATG TGTTCCAGTA TGAGCTGAAC AACCCCATGG CTTTGTATGG GTCTGTGCCT 
GTGCTCTTGG CACACAGCTT TCATCGGGAC TTGGGTATCT TCTGGCTTAA TGCTGCTGAG 
ACATGGGTTG ATATATCGTC CAACACAGCT GGGAAGACCC TGTTTGGGAA GATGCTGGAT 
TACCTGCAGG GGTCTGGGGA GACTCCACAG ACAGACATTC GTTGGATGTC CGAGAGCGGC 
ATTGTTGATG TTTTCCTCAT GCTCGGCCCT TCAGTCCTTG ATGTCTTCCG GCAGCATGCT 
AGCCTTACAG GGACCCAGGC ACTACCCCCA CTCTTCTCCC TGGGCTACCA CCAGAGTCGC 
TGGAACTACC GGGATGAGGC TGATGTTTTG GAAGTGGATC AGGGTTTTGA TGATCACAAC 
ATGCCTTGTG ATGTGATATG GTTGGACATT GAACATGCTG ATGGCAAGCG GTACTTCACT 
TGGGACCCCA CCCGATTTCC TCAGCCCCTC AATATGCTTG AGCACTTGGC TTCTAAAAGG 
CGGAAGCTGG TGGCCATCGT GGATCCCCAC ATCAAGGTAG ACTCTGGTTA CCGAGTTCAT 
GAAGAATTGC GAAACCATGG GCTGTATGTT AAAACTCGGG ATGGCTCTGA TTACGAGGGC 
TGGTGCTGGC CAGGTTCGGC TAGTTACCCT GACTTCACTA ACCCACAGAT GAGGGCCTGG 
TGGGCTAACA TGTTCAACTT TCACAACTAT GAGGGTTCAG CTCCTAATCT TTTTGTCTGG 
AATGACATGA ATGAACCGTC TGTGTTCAAT GGTCCTGAAG TCACCATGCT GAAGGATGCT 
GTGCATTATG GCGGCTGGGA GCACCGGGAC ATCCATAACA TCTATGGCTT ATATGTGCAC 
ATGGCAACTG CTGACGGGCT AATACAGCGC TCTGGTGGCA TAGAGCGGCC CTTTGTCCTG 
AGTAGGGCCT TCTTCTCAGG CTCCCAGCGC TTTGGAGCTG TGTGGACAGG GGACAACACT 
GCAGAGTGGG ATCATTTGAA GATCTCTATC CCTATGTGTC TCAGCCTGGC ACTGGTGGGG 
CTTTCCTTCT GTGGCGCGGA TGTGGGTGGC TTCTTCAAGA ACCCAGAGCC AGAACTGCTT 
GTGCGCTGGT ACCAAATGGG TGCCTACCAG CCATTCTTTC GGGCTCATGC TCACTTGGAC 
ACTGGGCGGC GAGAGCCGTG GCTGTTAGCG TCTCAATACC AAGATGCAAT CCGAGATGCC 
TTGTTCCAGC GATACTCTTT GCTGCCCTTC TGGTATACCC TCTTCTATCA AGCTCACCAG 
GAAGGGTTTC CTGTCATGAG GCCCCTCTGG GTACAGTATC CTGAGGATAT GTCTACCTTC 
AGTATGGAGG ATCAGTTCAT GCTTGGGGAC GCACTCCTTA TTCACCCTGT GTCGGATGCT 
GGGGCCCATG GAGTGCAGGT CTATTTGCCT GGCGAAGAAG AGGTGTGGTA TGACATTCAA 
AGCTATCAGA AGCATCATGG TCCCCAGACC TTGTATCTGC CAGTGACCCT GAGCAGTATA 
CCTGTGTTCC AGCGTGGCGG GACAATTGTC CCTCGATGGA TGCGCGTGAG GCGTTCTTCA 
GACTGTATGA AGGAAGATCC TATCACTCTC TTTGTTGCTC TCAGTGCCCA GGGCACGGCC 
CAAGGAGAGC TCTTTCTCGA TGATGGACAC ACTTTTAACT ATCAGAATCG CCATGAGTTC 
CTGTTGCGTC GATTCTCTTT CTCTGGCAAC ACACTTGTCT CTAGTTCAGC AGACCCCAAA 
GGCCACCTTG AGACACCTGT CTGGATTGAG CGAGTGGTCA TAATGGGGGC TGGAAAGCCA 
GCAGCCGTGG TGCTCCAGAC CAAAGGCTCT CCTGAAAGTC GCCTGTCCTT CCAGCATGAC 
CCTGAGACCT CTGTGCTGAC ATTGCGTAAA CCTGGCGTCA ATGTGGCATC TGACTGGAGT 
ATTCATCTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231039.1
CDS	70..2904
Translation	MAAIAAVAARRRRSWLSLVLAYLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDTLQLGPDALTVHLTHEVTKVLLVLELQGLQKNMTRIRIDELEPRRPRYRVPDVLVADPATARLSISGRDDNSVELTVAEGPYKIILTAKPFRLDLLEDRSLLLSVNARGLMAFEHQRAPRVPQEPKDAAEGNGAEPEARPGDGDKPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVYGIPEHADSLRLKVTEGGEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRDLGIFWLNAAETWVDISSNTAGKTLFGKMLDYLQGSGETPQTDIRWMSESGIVDVFLMLGPSVLDVFRQHASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYVKTRDGSDYEGWCWPGSASYPDFTNPQMRAWWANMFNFHNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHQEGFPVMRPLWVQYPEDMSTFSMEDQFMLGDALLIHPVSDAGAHGVQVYLPGEEEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKEDPITLFVALSAQGTAQGELFLDDGHTFNYQNRHEFLLRRFSFSGNTLVSSSADPKGHLETPVWIERVVIMGAGKPAAVVLQTKGSPESRLSFQHDPETSVLTLRKPGVNVASDWSIHLR

Target ORF information:

RefSeq Version	XM_006230977.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230977.3

ORF Insert Sequence:

ATGGCGGCGA TAGCGGCAGT GGCGGCGCGT AGGAGGCGGT CTTGGTTAAG TTTGGTGCTG 
GCATACTTAG GGGTCTGTCT GGGGATTACT CTTGCTGTGG ATAGAAGCAA CTTTAAGACC 
TGTGAAGAGA GTTCCTTTTG CAAACGGCAG CGAAGCATTC GGCCAGGCCT CTCTCCTTAC 
CGCGCCTTGC TGGACACTCT GCAGCTTGGT CCTGATGCTC TTACAGTCCA TCTGACCCAT 
GAAGTCACCA AGGTGTTGCT TGTGCTGGAG CTCCAGGGCC TTCAGAAGAA CATGACTCGG 
ATCAGGATCG ATGAGCTAGA GCCCCGGCGG CCTCGATACC GAGTGCCAGA TGTTTTAGTG 
GCTGACCCCG CCACAGCTCG GCTTTCGATT TCTGGCCGTG ATGACAACAG TGTGGAGCTA 
ACAGTGGCTG AAGGACCTTA CAAAATCATT TTGACAGCAA AGCCATTCCG CCTTGACCTG 
CTAGAAGACC GAAGCCTCCT GCTCAGTGTC AATGCCCGTG GACTTATGGC TTTTGAGCAT 
CAGAGGGCTC CCAGGGTCCC GCAAGAACCA AAAGATGCAG CTGAAGGCAA CGGAGCCGAG 
CCTGAAGCAA GGCCTGGGGA TGGTGACAAG CCAGAGGAGA CCCAGGAAAA GGCTGAGAAA 
GATGAGCCAG GAGCCTGGGA GGAGACATTC AAAACACACT CTGACAGCAA GCCTTACGGC 
CCCACGTCGG TAGGTTTGGA CTTTTCTCTG CCAGGAATGG AACATGTGTA TGGGATCCCT 
GAGCATGCAG ACAGCCTGAG ACTGAAGGTC ACTGAGGGTG GTGAGCCGTA CCGTCTGTAC 
AACTTGGATG TGTTCCAGTA TGAGCTGAAC AACCCCATGG CTTTGTATGG GTCTGTGCCT 
GTGCTCTTGG CACACAGCTT TCATCGGGAC TTGGGTATCT TCTGGCTTAA TGCTGCTGAG 
ACATGGGTTG ATATATCGTC CAACACAGCT GGGAAGACCC TGTTTGGGAA GATGCTGGAT 
TACCTGCAGG GGTCTGGGGA GACTCCACAG ACAGACATTC GTTGGATGTC CGAGAGCGGC 
ATTGTTGATG TTTTCCTCAT GCTCGGCCCT TCAGTCCTTG ATGTCTTCCG GCAGCATGCT 
AGCCTTACAG GGACCCAGGC ACTACCCCCA CTCTTCTCCC TGGGCTACCA CCAGAGTCGC 
TGGAACTACC GGGATGAGGC TGATGTTTTG GAAGTGGATC AGGGTTTTGA TGATCACAAC 
ATGCCTTGTG ATGTGATATG GTTGGACATT GAACATGCTG ATGGCAAGCG GTACTTCACT 
TGGGACCCCA CCCGATTTCC TCAGCCCCTC AATATGCTTG AGCACTTGGC TTCTAAAAGG 
CGGAAGCTGG TGGCCATCGT GGATCCCCAC ATCAAGGTAG ACTCTGGTTA CCGAGTTCAT 
GAAGAATTGC GAAACCATGG GCTGTATGTT AAAACTCGGG ATGGCTCTGA TTACGAGGGC 
TGGTGCTGGC CAGGTTCGGC TAGTTACCCT GACTTCACTA ACCCACAGAT GAGGGCCTGG 
TGGGCTAACA TGTTCAACTT TCACAACTAT GAGGGTTCAG CTCCTAATCT TTTTGTCTGG 
AATGACATGA ATGAACCGTC TGTGTTCAAT GGTCCTGAAG TCACCATGCT GAAGGATGCT 
GTGCATTATG GCGGCTGGGA GCACCGGGAC ATCCATAACA TCTATGGCTT ATATGTGCAC 
ATGGCAACTG CTGACGGGCT AATACAGCGC TCTGGTGGCA TAGAGCGGCC CTTTGTCCTG 
AGTAGGGCCT TCTTCTCAGG CTCCCAGCGC TTTGGAGCTG TGTGGACAGG GGACAACACT 
GCAGAGTGGG ATCATTTGAA GATCTCTATC CCTATGTGTC TCAGCCTGGC ACTGGTGGGG 
CTTTCCTTCT GTGGCGCGGA TGTGGGTGGC TTCTTCAAGA ACCCAGAGCC AGAACTGCTT 
GTGCGCTGGT ACCAAATGGG TGCCTACCAG CCATTCTTTC GGGCTCATGC TCACTTGGAC 
ACTGGGCGGC GAGAGCCGTG GCTGTTAGCG TCTCAATACC AAGATGCAAT CCGAGATGCC 
TTGTTCCAGC GATACTCTTT GCTGCCCTTC TGGTATACCC TCTTCTATCA AGCTCACCAG 
GAAGGGTTTC CTGTCATGAG GCCCCTCTGG GTACAGTATC CTGAGGATAT GTCTACCTTC 
AGTATGGAGG ATCAGTTCAT GCTTGGGGAC GCACTCCTTA TTCACCCTGT GTCGGATGCT 
GGGGCCCATG GAGTGCAGGT CTATTTGCCT GGCGAAGAAG AGGTGTGGTA TGACATTCAA 
AGCTATCAGA AGCATCATGG TCCCCAGACC TTGTATCTGC CAGTGACCCT GAGCAGTATA 
CCTGTGTTCC AGCGTGGCGG GACAATTGTC CCTCGATGGA TGCGCGTGAG GCGTTCTTCA 
GACTGTATGA AGGAAGATCC TATCACTCTC TTTGTTGCTC TCAGTGCCCA GGGCACGGCC 
CAAGGAGAGC TCTTTCTCGA TGATGGACAC ACTTTTAACT ATCAGAATCG CCATGAGTTC 
CTGTTGCGTC GATTCTCTTT CTCTGGCAAC ACACTTGTCT CTAGTTCAGC AGACCCCAAA 
GGCCACCTTG AGACACCTGT CTGGATTGAG CGAGTGGTCA TAATGGGGGC TGGAAAGCCA 
GCAGCCGTGG TGCTCCAGAC CAAAGGCTCT CCTGAAAGTC GCCTGTCCTT CCAGCATGAC 
CCTGAGACCT CTGTGCTGAC ATTGCGTAAA CCTGGCGTCA ATGTGGCATC TGACTGGAGT 
ATTCATCTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phenotypically Silent Bone Morphogenetic Protein Receptor 2 Mutations Predispose Rats to Inflammation-Induced Pulmonary Arterial Hypertension by Enhancing the Risk for Neointimal Transformation.
Circulation140(17)1409-1425(2019 Oct)
Tian W,Jiang X,Sung YK,Shuffle E,Wu TH,Kao PN,Tu AB,Dorfmüller P,Cao A,Wang L,Peng G,Kim Y,Zhang P,Chappell J,Pasupneti S,Dahms P,Maguire P,Chaib H,Zamanian R,Peters-Golden M,Snyder MP,Voelkel NF,Humbert M,Rabinovitch M,Nicolls MR

Isolated polycystic liver disease genes define effectors of polycystin-1 function.
The Journal of clinical investigation127(5)1772-1785(2017 May)
Besse W,Dong K,Choi J,Punia S,Fedeles SV,Choi M,Gallagher AR,Huang EB,Gulati A,Knight J,Mane S,Tahvanainen E,Tahvanainen P,Sanna-Cherchi S,Lifton RP,Watnick T,Pei YP,Torres VE,Somlo S

Structures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)
Caputo AT,Alonzi DS,Marti L,Reca IB,Kiappes JL,Struwe WB,Cross A,Basu S,Lowe ED,Darlot B,Santino A,Roversi P,Zitzmann N

Mutations in GANAB, Encoding the Glucosidase IIα Subunit, Cause Autosomal-Dominant Polycystic Kidney and Liver Disease.
American journal of human genetics98(6)1193-1207(2016 06)
Porath B,Gainullin VG,Cornec-Le Gall E,Dillinger EK,Heyer CM,Hopp K,Edwards ME,Madsen CD,Mauritz SR,Banks CJ,Baheti S,Reddy B,Herrero JI,Bañales JM,Hogan MC,Tasic V,Watnick TJ,Chapman AB,Vigneau C,Lavainne F,Audrézet MP,Ferec C,Le Meur Y,Torres VE,,,Harris PC

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hernández-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

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Ganab cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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