GCS2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCS2alpha
Entrez Gene ID	49953
Full Name	Glucosidase 2 alpha subunit
Synonyms	BcDNA:GH04962,CG14476,Dmel\CG14476,clot#312
General protein information	Preferred Names Glucosidase 2 alpha subunit Names CG14476 gene product from transcript CG14476-RC CG14476-PA CG14476-PB CG14476-PC CG14476-PD CG14476-PE GCS2alpha-PA GCS2alpha-PB GCS2alpha-PC GCS2alpha-PD GCS2alpha-PE alpha-glucosidase glucosidase 2 alpha subunit glucosidase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 20A4-20A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_167759.2	NP_728436.1	glucosidase 2 alpha subunit, isoform D
NM_167758.2	NP_728435.1	glucosidase 2 alpha subunit, isoform C
NM_167757.3	NP_728434.1	glucosidase 2 alpha subunit, isoform A
NM_167760.1	NP_728437.1	glucosidase 2 alpha subunit, isoform E
NM_143888.5	NP_652145.1	glucosidase 2 alpha subunit, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	GANAB	NP_938149.2
Bos taurus (cattle)	GANAB	NP_001192706.1
Drosophila melanogaster (fruit fly)	CG14476	NP_652145.1
Caenorhabditis elegans (roundworm)	aagr-4	NP_508105.2
Saccharomyces cerevisiae (baker's yeast)	ROT2	NP_009788.3
Neurospora crassa	NCU04203	XP_961163.2
Xenopus tropicalis (tropical clawed frog)	ganab	XP_004919758.1
Oryza sativa (rice)	Os03g0216600	NP_001049385.1
Canis lupus familiaris (dog)	GANAB	XP_540905.2
Mus musculus (house mouse)	Ganab	NP_032086.1
Danio rerio (zebrafish)	LOC100334924	XP_002664508.2
Pan troglodytes (chimpanzee)	GANAB	XP_508494.2
Caenorhabditis elegans (roundworm)	aagr-3	NP_505507.1
Eremothecium gossypii	AGOS_AAR173C	NP_982716.1
Kluyveromyces lactis	KLLA0F09735g	XP_455522.1
Macaca mulatta (Rhesus monkey)	GANAB	XP_001116396.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000862	XP_316832.4
Schizosaccharomyces pombe (fission yeast)	gls2	NP_593490.1
Magnaporthe oryzae (rice blast fungus)	MGG_08623	XP_003711051.1
Rattus norvegicus (Norway rat)	Ganab	NP_001099804.1
Arabidopsis thaliana (thale cress)	RSW3	NP_201189.1
Danio rerio (zebrafish)	ganab	XP_002664506.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCS2alpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCS2alpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02425	NM_167759.2 Latest version!	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant D (GCS2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa02425	NM_167758.2 Latest version!	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant C (GCS2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa02425	NM_143888.5 Latest version!	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant B (GCS2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa02425	NM_167760.1 Latest version!	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant E (GCS2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa02425	NM_167757.3 Latest version!	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant A (GCS2alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCS2alpha ( NM_167759.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728436.1 Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant D (GCS2alpha), mRNA.

Price & Availability↑

CloneID	OFa02425
Clone ID Related Accession (Same CDS sequence)	NM_167759.2 , NM_167758.2 , NM_167757.3 , NM_167760.1 , NM_143888.5
Accession Version	NM_167759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glucosidase 2 alpha subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167759.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728436.1
CDS	112..2886
Translation	MRFALATVAIIWALFVLADGVDPGNFKTCEQSSFCRRSRKIQGSGSKYALIPGTLNTYADSLTADLVNKENHHQFAFKLEALVGSTFRLQIDEKQPLRPRYRVEHALKGPTQAGRIRVQRETDGEIVITSEKNKAVIHGDPFRIDFFENDVLVVSVNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENFKSHHDSKPYGPEAVALDFSFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVFNGPEITAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNSFVGKPKYKTLAWIERIVIAGLEKVPSSASITVNGVSQQLEVLHHDNTVVVRKPGVKMDVDFAIKLNFA

Target ORF information:

RefSeq Version	NM_167759.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant D (GCS2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167759.2

ORF Insert Sequence:

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCS2alpha ( NM_167758.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728435.1 Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant C (GCS2alpha), mRNA.

Price & Availability↑

CloneID	OFa02425
Clone ID Related Accession (Same CDS sequence)	NM_167759.2 , NM_167758.2 , NM_167757.3 , NM_167760.1 , NM_143888.5
Accession Version	NM_167758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glucosidase 2 alpha subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167758.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728435.1
CDS	137..2911
Translation	MRFALATVAIIWALFVLADGVDPGNFKTCEQSSFCRRSRKIQGSGSKYALIPGTLNTYADSLTADLVNKENHHQFAFKLEALVGSTFRLQIDEKQPLRPRYRVEHALKGPTQAGRIRVQRETDGEIVITSEKNKAVIHGDPFRIDFFENDVLVVSVNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENFKSHHDSKPYGPEAVALDFSFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVFNGPEITAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNSFVGKPKYKTLAWIERIVIAGLEKVPSSASITVNGVSQQLEVLHHDNTVVVRKPGVKMDVDFAIKLNFA

Target ORF information:

RefSeq Version	NM_167758.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant C (GCS2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167758.2

ORF Insert Sequence:

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCS2alpha ( NM_167757.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728434.1 Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant A (GCS2alpha), mRNA.

Price & Availability↑

CloneID	OFa02425
Clone ID Related Accession (Same CDS sequence)	NM_167759.2 , NM_167758.2 , NM_167757.3 , NM_167760.1 , NM_143888.5
Accession Version	NM_167757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glucosidase 2 alpha subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167757.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728434.1
CDS	211..2985
Translation	MRFALATVAIIWALFVLADGVDPGNFKTCEQSSFCRRSRKIQGSGSKYALIPGTLNTYADSLTADLVNKENHHQFAFKLEALVGSTFRLQIDEKQPLRPRYRVEHALKGPTQAGRIRVQRETDGEIVITSEKNKAVIHGDPFRIDFFENDVLVVSVNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENFKSHHDSKPYGPEAVALDFSFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVFNGPEITAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNSFVGKPKYKTLAWIERIVIAGLEKVPSSASITVNGVSQQLEVLHHDNTVVVRKPGVKMDVDFAIKLNFA

Target ORF information:

RefSeq Version	NM_167757.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant A (GCS2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167757.3

ORF Insert Sequence:

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCS2alpha ( NM_167760.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728437.1 Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant E (GCS2alpha), mRNA.

Price & Availability↑

CloneID	OFa02425
Clone ID Related Accession (Same CDS sequence)	NM_167759.2 , NM_167758.2 , NM_167757.3 , NM_167760.1 , NM_143888.5
Accession Version	NM_167760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glucosidase 2 alpha subunit, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728437.1
CDS	247..3021
Translation	MRFALATVAIIWALFVLADGVDPGNFKTCEQSSFCRRSRKIQGSGSKYALIPGTLNTYADSLTADLVNKENHHQFAFKLEALVGSTFRLQIDEKQPLRPRYRVEHALKGPTQAGRIRVQRETDGEIVITSEKNKAVIHGDPFRIDFFENDVLVVSVNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENFKSHHDSKPYGPEAVALDFSFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVFNGPEITAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNSFVGKPKYKTLAWIERIVIAGLEKVPSSASITVNGVSQQLEVLHHDNTVVVRKPGVKMDVDFAIKLNFA

Target ORF information:

RefSeq Version	NM_167760.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant E (GCS2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167760.1

ORF Insert Sequence:

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCS2alpha ( NM_143888.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652145.1 Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant B (GCS2alpha), mRNA.

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CloneID	OFa02425
Clone ID Related Accession (Same CDS sequence)	NM_167759.2 , NM_167758.2 , NM_167757.3 , NM_167760.1 , NM_143888.5
Accession Version	NM_143888.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glucosidase 2 alpha subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_143888.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652145.1
CDS	115..2889
Translation	MRFALATVAIIWALFVLADGVDPGNFKTCEQSSFCRRSRKIQGSGSKYALIPGTLNTYADSLTADLVNKENHHQFAFKLEALVGSTFRLQIDEKQPLRPRYRVEHALKGPTQAGRIRVQRETDGEIVITSEKNKAVIHGDPFRIDFFENDVLVVSVNAKNWLYFEHLRQKAQEPTSHPAENENQQEQDAAVETPKAADTIDDPGAWEENFKSHHDSKPYGPEAVALDFSFPAAKVLFGIPEHADSFILKSTSGTDPYRLYNLDVFEYVVDSKMALYGSVPVIYGHGAQRTAGVYWQNAAETWVDIQTSETNVVSSLVNFVSGSQKTPPPAAHFMSESGIVDAFIMLGPKPMDTFKQYAALTGTHELPQLFALAYHQSRWNYNDERDVTSVSAKFDEYNIPMDTMWLDIEHTDGKRYFTWDKFKFPHPLAMIKNLTELGRHLVVIVDPHIKRDNNYFFHRDCTDRGYYVKTREGNDYEGWCWPGAASYPDFFNPVVREYYASQYALDKFQTVTADVMLWNDMNEPSVFNGPEITAPKDLIHYGNWEHRDVHNLYGHMHLMGSFAGLQQRDPNQRPFILTRAHFAGSQRYAAIWTGDNFADWSHLQHSVKMCLTEAVAGFSFCGADVGAFFGNPDTELLERWYQTGAFLPFFRAHAHIDTKRREPWLFPERTRQVIQNAVIKRYSYLPLWYTAFYELELTGEPVIRPLLAQYPLDKEAFGVDNQLLVQDRLLVRPVMQQGVSKVDVYFPAIDDKKNGDWWYDVDTYQRQERSGYVSVPVDDFKIPVWQRGGSIVPKKERQRRASTLMLHDPYTLIICLDRQGKASGSLYLDDEKSYAYRQGQRIHVNYEFAHDQLVNSFVGKPKYKTLAWIERIVIAGLEKVPSSASITVNGVSQQLEVLHHDNTVVVRKPGVKMDVDFAIKLNFA

Target ORF information:

RefSeq Version	NM_143888.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glucosidase 2 alpha subunit, transcript variant B (GCS2alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143888.5

ORF Insert Sequence:

ATGCGATTCG CTCTGGCCAC CGTGGCCATC ATCTGGGCTT TATTCGTCCT GGCCGACGGA 
GTGGACCCCG GGAACTTTAA GACCTGCGAG CAGAGCAGTT TTTGTCGACG CTCCCGAAAG 
ATTCAGGGAT CGGGCAGCAA GTACGCCTTA ATTCCCGGCA CCTTGAACAC CTATGCGGAT 
TCGTTGACCG CTGATCTCGT TAACAAGGAA AACCATCACC AGTTTGCCTT TAAGCTAGAG 
GCTCTGGTGG GCAGTACCTT CCGCCTGCAG ATCGATGAAA AGCAGCCACT TCGGCCACGA 
TATCGCGTCG AGCACGCTCT GAAGGGCCCA ACCCAGGCCG GACGCATTCG TGTCCAGCGG 
GAAACAGATG GAGAGATCGT TATCACATCG GAGAAGAACA AGGCTGTGAT CCACGGAGAC 
CCGTTCCGCA TCGATTTCTT CGAGAACGAC GTGCTGGTGG TCTCGGTGAA TGCCAAGAAC 
TGGTTGTACT TTGAGCATCT GCGGCAGAAG GCCCAGGAAC CGACCTCCCA CCCGGCGGAA 
AACGAGAATC AGCAGGAGCA GGATGCCGCT GTCGAAACTC CTAAAGCAGC AGATACTATC 
GATGATCCTG GTGCATGGGA GGAGAACTTC AAATCACACC ACGACTCTAA GCCCTACGGT 
CCGGAAGCTG TGGCTCTGGA CTTTTCGTTC CCCGCAGCCA AAGTTCTATT CGGTATTCCA 
GAGCACGCCG ACAGCTTCAT ACTAAAGTCC ACCTCGGGTA CGGATCCTTA CCGCCTGTAC 
AACCTGGATG TGTTCGAATA CGTTGTGGAC AGCAAGATGG CCCTGTACGG CTCAGTGCCG 
GTAATCTATG GTCATGGAGC GCAACGCACA GCGGGCGTTT ACTGGCAGAA TGCCGCTGAA 
ACTTGGGTGG ACATTCAAAC GTCGGAGACC AATGTTGTAT CTTCGCTGGT AAATTTTGTA 
TCCGGCTCGC AGAAGACTCC TCCTCCGGCT GCCCACTTTA TGTCGGAGTC GGGCATCGTG 
GACGCCTTCA TCATGTTGGG ACCCAAGCCC ATGGACACTT TTAAGCAGTA CGCGGCTCTG 
ACTGGAACAC ACGAGTTACC TCAGTTATTT GCTCTGGCCT ACCACCAGAG CCGCTGGAAC 
TACAACGACG AGAGGGACGT GACCTCCGTG TCAGCGAAAT TCGACGAGTA CAATATACCC 
ATGGACACCA TGTGGCTGGA CATCGAGCAT ACAGATGGCA AGCGCTACTT CACCTGGGAC 
AAGTTCAAGT TCCCGCACCC GCTGGCAATG ATCAAGAATC TTACAGAACT GGGTCGCCAC 
TTGGTGGTGA TAGTCGACCC GCACATCAAG CGGGATAATA ATTACTTCTT CCACCGCGAC 
TGCACGGATC GTGGTTATTA TGTGAAAACG CGCGAGGGCA ATGATTACGA GGGCTGGTGT 
TGGCCGGGAG CGGCTAGTTA TCCTGATTTC TTTAACCCCG TGGTCAGAGA GTACTACGCC 
AGCCAGTACG CGCTGGACAA GTTCCAAACG GTCACCGCAG ATGTGATGTT GTGGAACGAC 
ATGAACGAGC CATCGGTGTT CAACGGCCCT GAGATCACGG CGCCCAAGGA CTTGATCCAC 
TACGGCAACT GGGAGCACCG TGACGTGCAC AATTTGTACG GTCACATGCA CTTGATGGGG 
TCCTTCGCGG GCCTGCAGCA GCGCGATCCC AACCAGCGGC CCTTCATCCT CACACGCGCC 
CACTTTGCTG GTTCCCAGCG TTATGCTGCC ATTTGGACGG GTGACAACTT TGCGGACTGG 
TCGCATCTAC AGCACTCAGT CAAAATGTGT CTCACGGAGG CGGTGGCCGG CTTCTCCTTC 
TGCGGTGCCG ATGTCGGTGC CTTCTTTGGA AACCCAGATA CTGAGCTGCT GGAGCGCTGG 
TATCAAACCG GTGCCTTCCT GCCCTTTTTC CGGGCTCATG CACACATCGA CACTAAGCGC 
CGGGAGCCAT GGCTTTTCCC AGAACGCACT CGCCAGGTTA TCCAAAATGC TGTGATCAAG 
CGTTATTCAT ATCTGCCCCT GTGGTACACC GCATTCTACG AGCTGGAGCT GACTGGCGAG 
CCGGTAATTC GCCCATTATT AGCCCAATAT CCACTGGATA AGGAGGCCTT CGGCGTTGAC 
AACCAGCTGT TGGTGCAAGA TCGCCTGCTT GTGAGACCCG TCATGCAGCA GGGCGTCAGC 
AAGGTGGACG TGTACTTCCC GGCAATTGAC GACAAAAAGA ATGGCGACTG GTGGTACGAT 
GTGGATACTT ACCAGCGCCA GGAACGATCC GGCTATGTGT CGGTTCCAGT AGATGATTTC 
AAGATCCCCG TTTGGCAGCG TGGTGGCAGC ATTGTACCGA AGAAGGAGCG ACAGCGCCGC 
GCCTCAACCT TGATGTTGCA CGATCCGTAC ACACTGATAA TCTGCTTAGA CCGTCAGGGC 
AAGGCATCCG GTTCCCTCTA CCTAGACGAC GAGAAGTCCT ACGCTTACCG CCAGGGCCAG 
CGCATCCACG TGAACTACGA GTTCGCCCAC GACCAGCTGG TCAATAGCTT CGTGGGCAAG 
CCCAAGTACA AGACCCTTGC CTGGATCGAG CGAATCGTGA TCGCCGGACT TGAGAAGGTG 
CCCAGCAGCG CCAGCATCAC GGTGAATGGT GTCAGCCAGC AGCTGGAGGT GCTCCATCAT 
GATAACACCG TCGTCGTGCG CAAGCCGGGC GTCAAGATGG ACGTCGACTT CGCCATTAAA 
CTCAACTTTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GCS2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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