Chkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chkb
Entrez Gene ID	29367
Full Name	choline kinase beta
Synonyms	Chetk,Chkl
General protein information	Preferred Names choline kinase beta Names choline/ethanolamine kinase CK CKB CKEKB EK choline/ethanolamine kinase beta ethanolamine kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	choline/ethanolamine kinase enzyme [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765677.2	XP_008763899.1	choline/ethanolamine kinase isoform X2
XM_006242181.3	XP_006242243.2	choline/ethanolamine kinase isoform X1
NM_017177.1	NP_058873.1	choline/ethanolamine kinase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0535400	NP_001174502.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007957	XP_317515.4
Macaca mulatta (Rhesus monkey)	LOC716377	XP_002808018.1
Canis lupus familiaris (dog)	CHKB	XP_848257.1
Drosophila melanogaster (fruit fly)	CG2201	NP_724349.1
Xenopus tropicalis (tropical clawed frog)	chkb	NP_001120148.1
Pan troglodytes (chimpanzee)	CHKB	XP_001144556.1
Bos taurus (cattle)	CHKB	NP_001193023.1
Caenorhabditis elegans (roundworm)	cka-1	NP_001129849.1
Rattus norvegicus (Norway rat)	Chkb	NP_058873.1
Danio rerio (zebrafish)	chkb	NP_001093482.1
Oryza sativa (rice)	Os01g0717000	NP_001044073.1
Homo sapiens (human)	CHKB	NP_005189.2
Mus musculus (house mouse)	Chkb	NP_031718.1
Arabidopsis thaliana (thale cress)	AT1G74320	NP_177572.2

Gene Ontology
Bibliography

Related articles in PubMed

Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy.
Mitsuhashi S, Hatakeyama H, Karahashi M, Koumura T, Nonaka I, Hayashi YK, Noguchi S, Sher RB, Nakagawa Y, Manfredi G, Goto Y, Cox GA, Nishino I
Human molecular genetics20(19)3841-51(2011 Oct)

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis.
Mitsuhashi S, Ohkuma A, Talim B, Karahashi M, Koumura T, Aoyama C, Kurihara M, Quinlivan R, Sewry C, Mitsuhashi H, Goto K, Koksal B, Kale G, Ikeda K, Taguchi R, Noguchi S, Hayashi YK, Nonaka I, Sher RB, Sugimoto H, Nakagawa Y, Cox GA, Topaloglu H, Nishino I
American journal of human genetics88(6)845-851(2011 Jun)

Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment.
Gallego-Ortega D, Ramirez de Molina A, Ramos MA, Valdes-Mora F, Barderas MG, Sarmentero-Estrada J, Lacal JC
PloS one4(11)e7819(2009 Nov)

Polymorphism located between CPT1B and CHKB, and HLA-DRB1*1501-DQB1*0602 haplotype confer susceptibility to CNS hypersomnias (essential hypersomnia).
Miyagawa T, Honda M, Kawashima M, Shimada M, Tanaka S, Honda Y, Tokunaga K
PloS one4(4)e5394(2009)

Variant between CPT1B and CHKB associated with susceptibility to narcolepsy.
Miyagawa T, Kawashima M, Nishida N, Ohashi J, Kimura R, Fujimoto A, Shimada M, Morishita S, Shigeta T, Lin L, Hong SC, Faraco J, Shin YK, Jeong JH, Okazaki Y, Tsuji S, Honda M, Honda Y, Mignot E, Tokunaga K
Nature genetics40(11)1324-8(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03131	NM_017177.1 Latest version!	Rattus norvegicus choline kinase beta (Chkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa43257	XM_006242181.3 Latest version!	Rattus norvegicus choline kinase beta (Chkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa43258	XM_008765677.2 Latest version!	Rattus norvegicus choline kinase beta (Chkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chkb ( NM_017177.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058873.1 Rattus norvegicus choline kinase beta (Chkb), mRNA.

Price & Availability↑

CloneID	ORa03131
Clone ID Related Accession (Same CDS sequence)	NM_017177.1
Accession Version	NM_017177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	choline/ethanolamine kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006607.1. ##Evidence-Data-START## Transcript exon combination :: AB006607.1, BC060515.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGCGGAGG CCTCAGCAAC 
CTGCTCTTCC GATGCTCGCT ACCGAACCAC GTGCCCAGTA TGGGCGGGGA GCCCCGGGAG 
GTGCTGCTAC GGCTGTACGG GGCTATCTTG CAGGGTGTAG ACTCCTTGGT ATTAGAAAGC 
GTGATGTTCG CCATTCTTGC AGAGAGATCT CTAGGGCCCC AACTTTATGG AGTGTTTCCA 
GAGGGCCGCT TGGAACAGTA CCTCCCAAGC CGGCCATTGA AAACTCAAGA GCTCCGGGAC 
CCAGTGTTGT CAGGAGCCAT TGCAACAAAG ATGGCCCGTT TCCATGGTAT GGAGATGCCC 
TTCACCAAGG AGCCCCGCTG GTTGTTTGGG ACCATGGAGC GGTACCTAAA GCAGATCCAG 
GACCTGCCGT CCACTAGCCT TCCCCAGATG AACCTGGTGG AGATGTACAG CCTCAAGGAT 
GAGATGAATC ACCTCAGGAC GTTGCTAGAC GCTACACCGT CCCCAGTGGT CTTCTGCCAC 
AATGACATCC AGGAAGGAAA CATCTTACTG CTCTCAGAGC CAGACAGTGA TGACAACCTC 
ATGTTGGTTG ATTTCGAGTA CAGTAGTTAC AACTACAGGG GCTTTGACAT TGGGAATCAT 
TTCTGTGAGT GGGTTTACGA TTACACTTAC GAGGAGTGGC CTTTCTACAA AGCAAGACCT 
GCAGACTACC CCACTAGAGA ACAGCAGCTC CTTTTCATCC GTCATTATCT GGCGGAGGTT 
CAGAAAGGTG AGGTCCTCTC CGAAGAGGAG CAGAAGAAAC AGGAAGAAGA TTTGCTGATA 
GAGATCAGCC GGTATGCCCT GGCCTCTCAT TTCTTCTGGG GCCTATGGTC CACCCTCCAG 
GCTTCCATGT CCACTATAGA GTTTGGCTAC TTGGAATACG CCCAATCTCG GTTCCAGTTC 
TACTTCCAGC AGAAGGGGCA GCTGACCAGC TTCCTATCAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058873.1
CDS	279..1463
Translation	MAADGTGVVGGGAVGGPLSKDGLLDAKCPEPIPNRRRSSSLSRDAQRRAYQWCREYLGGAWRRARPEELSVCPVSGGLSNLLFRCSLPNHVPSMGGEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYLPSRPLKTQELRDPVLSGAIATKMARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQMNLVEMYSLKDEMNHLRTLLDATPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTYEEWPFYKARPADYPTREQQLLFIRHYLAEVQKGEVLSEEEQKKQEEDLLIEISRYALASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSFLSP

Target ORF information:

RefSeq Version	NM_017177.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus choline kinase beta (Chkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017177.1

ORF Insert Sequence:

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGCGGAGG CCTCAGCAAC 
CTGCTCTTCC GATGCTCGCT ACCGAACCAC GTGCCCAGTA TGGGCGGGGA GCCCCGGGAG 
GTGCTGCTAC GGCTGTACGG GGCTATCTTG CAGGGTGTAG ACTCCTTGGT ATTAGAAAGC 
GTGATGTTCG CCATTCTTGC AGAGAGATCT CTAGGGCCCC AACTTTATGG AGTGTTTCCA 
GAGGGCCGCT TGGAACAGTA CCTCCCAAGC CGGCCATTGA AAACTCAAGA GCTCCGGGAC 
CCAGTGTTGT CAGGAGCCAT TGCAACAAAG ATGGCCCGTT TCCATGGTAT GGAGATGCCC 
TTCACCAAGG AGCCCCGCTG GTTGTTTGGG ACCATGGAGC GGTACCTAAA GCAGATCCAG 
GACCTGCCGT CCACTAGCCT TCCCCAGATG AACCTGGTGG AGATGTACAG CCTCAAGGAT 
GAGATGAATC ACCTCAGGAC GTTGCTAGAC GCTACACCGT CCCCAGTGGT CTTCTGCCAC 
AATGACATCC AGGAAGGAAA CATCTTACTG CTCTCAGAGC CAGACAGTGA TGACAACCTC 
ATGTTGGTTG ATTTCGAGTA CAGTAGTTAC AACTACAGGG GCTTTGACAT TGGGAATCAT 
TTCTGTGAGT GGGTTTACGA TTACACTTAC GAGGAGTGGC CTTTCTACAA AGCAAGACCT 
GCAGACTACC CCACTAGAGA ACAGCAGCTC CTTTTCATCC GTCATTATCT GGCGGAGGTT 
CAGAAAGGTG AGGTCCTCTC CGAAGAGGAG CAGAAGAAAC AGGAAGAAGA TTTGCTGATA 
GAGATCAGCC GGTATGCCCT GGCCTCTCAT TTCTTCTGGG GCCTATGGTC CACCCTCCAG 
GCTTCCATGT CCACTATAGA GTTTGGCTAC TTGGAATACG CCCAATCTCG GTTCCAGTTC 
TACTTCCAGC AGAAGGGGCA GCTGACCAGC TTCCTATCAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chkb ( XM_006242181.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242243.2 Rattus norvegicus choline kinase beta (Chkb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43257
Clone ID Related Accession (Same CDS sequence)	XM_006242181.3
Accession Version	XM_006242181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline/ethanolamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGGTCAGG GGTCAGCCTC 
ACACTCCTCC GGCGGAGGCC TCAGCAACCT GCTCTTCCGA TGCTCGCTAC CGAACCACGT 
GCCCAGTATG GGCGGGGAGC CCCGGGAGGT GCTGCTACGG CTGTACGGGG CTATCTTGCA 
GGGTGTAGAC TCCTTGGTAT TAGAAAGCGT GATGTTCGCC ATTCTTGCAG AGAGATCTCT 
AGGGCCCCAA CTTTATGGAG TGTTTCCAGA GGGCCGCTTG GAACAGTACC TCCCAGTTAC 
TGGGTACAGA GCCGGCCATT GAAAACTCAA GAGCTCCGGG ACCCAGTGTT GTCAGGAGCC 
ATTGCAACAA AGATGGCCCG TTTCCATGGT ATGGAGATGC CCTTCACCAA GGAGCCCCGC 
TGGTTGTTTG GGACCATGGA GCGGTACCTA AAGCAGATCC AGGACCTGCC GTCCACTAGC 
CTTCCCCAGA TGAACCTGGT GGAGATGTAC AGCCTCAAGG ATGAGATGAA TCACCTCAGG 
ACGTTGCTAG ACGCTACACC GTCCCCAGTG GTCTTCTGCC ACAATGACAT CCAGGAAGGA 
AACATCTTAC TGCTCTCAGA GCCAGACAGT GATGACAACC TCATGTTGGT TGATTTCGAG 
TACAGTAGTT ACAACTACAG GGGCTTTGAC ATTGGGAATC ATTTCTGTGA GTGGGTTTAC 
GATTACACTT ACGAGGAGTG GCCTTTCTAC AAAGCAAGAC CTGCAGACTA CCCCACTAGA 
GAACAGCAGC TCCTTTTCAT CCGTCATTAT CTGGCGGAGG TTCAGAAAGG TGAGGTCCTC 
TCCGAAGAGG AGCAGAAGAA ACAGGAAGAA GATTTGCTGA TAGAGATCAG CCGGTATGCC 
CTGGCCTCTC ATTTCTTCTG GGGCCTATGG TCCACCCTCC AGGCTTCCAT GTCCACTATA 
GAGTTTGGCT ACTTGGAATA CGCCCAATCT CGGTTCCAGT TCTACTTCCA GCAGAAGGGG 
CAGCTGACCA GCTTCCTATC ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242243.2
CDS	905..2131
Translation	MAADGTGVVGGGAVGGPLSKDGLLDAKCPEPIPNRRRSSSLSRDAQRRAYQWCREYLGGAWRRARPEELSVCPVRSGVSLTLLRRRPQQPALPMLATEPRAQYGRGAPGGAATAVRGYLAGCRLLGIRKRDVRHSCREISRAPTLWSVSRGPLGTVPPSYWVQSRPLKTQELRDPVLSGAIATKMARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQMNLVEMYSLKDEMNHLRTLLDATPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTYEEWPFYKARPADYPTREQQLLFIRHYLAEVQKGEVLSEEEQKKQEEDLLIEISRYALASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSFLSP

Target ORF information:

RefSeq Version	XM_006242181.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus choline kinase beta (Chkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242181.3

ORF Insert Sequence:

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGGTCAGG GGTCAGCCTC 
ACACTCCTCC GGCGGAGGCC TCAGCAACCT GCTCTTCCGA TGCTCGCTAC CGAACCACGT 
GCCCAGTATG GGCGGGGAGC CCCGGGAGGT GCTGCTACGG CTGTACGGGG CTATCTTGCA 
GGGTGTAGAC TCCTTGGTAT TAGAAAGCGT GATGTTCGCC ATTCTTGCAG AGAGATCTCT 
AGGGCCCCAA CTTTATGGAG TGTTTCCAGA GGGCCGCTTG GAACAGTACC TCCCAGTTAC 
TGGGTACAGA GCCGGCCATT GAAAACTCAA GAGCTCCGGG ACCCAGTGTT GTCAGGAGCC 
ATTGCAACAA AGATGGCCCG TTTCCATGGT ATGGAGATGC CCTTCACCAA GGAGCCCCGC 
TGGTTGTTTG GGACCATGGA GCGGTACCTA AAGCAGATCC AGGACCTGCC GTCCACTAGC 
CTTCCCCAGA TGAACCTGGT GGAGATGTAC AGCCTCAAGG ATGAGATGAA TCACCTCAGG 
ACGTTGCTAG ACGCTACACC GTCCCCAGTG GTCTTCTGCC ACAATGACAT CCAGGAAGGA 
AACATCTTAC TGCTCTCAGA GCCAGACAGT GATGACAACC TCATGTTGGT TGATTTCGAG 
TACAGTAGTT ACAACTACAG GGGCTTTGAC ATTGGGAATC ATTTCTGTGA GTGGGTTTAC 
GATTACACTT ACGAGGAGTG GCCTTTCTAC AAAGCAAGAC CTGCAGACTA CCCCACTAGA 
GAACAGCAGC TCCTTTTCAT CCGTCATTAT CTGGCGGAGG TTCAGAAAGG TGAGGTCCTC 
TCCGAAGAGG AGCAGAAGAA ACAGGAAGAA GATTTGCTGA TAGAGATCAG CCGGTATGCC 
CTGGCCTCTC ATTTCTTCTG GGGCCTATGG TCCACCCTCC AGGCTTCCAT GTCCACTATA 
GAGTTTGGCT ACTTGGAATA CGCCCAATCT CGGTTCCAGT TCTACTTCCA GCAGAAGGGG 
CAGCTGACCA GCTTCCTATC ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chkb ( XM_008765677.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763899.1 Rattus norvegicus choline kinase beta (Chkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43258
Clone ID Related Accession (Same CDS sequence)	XM_008765677.2
Accession Version	XM_008765677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline/ethanolamine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGGTCAGG GGTCAGCCTC 
ACACTCCTCC GGGTTCGGTT GCCCGGAGGC CTCAGCAACC TGCTCTTCCG ATGCTCGCTA 
CCGAACCACG TGCCCAGTAT GGGCGGGGAG CCCCGGGAGG TGCTGCTACG GCTGTACGGG 
GCTATCTTGC AGGGTGTAGA CTCCTTGGTA TTAGAAAGCG TGATGTTCGC CATTCTTGCA 
GAGAGATCTC TAGGGCCCCA ACTTTATGGA GTGTTTCCAG AGGGCCGCTT GGAACAGTAC 
CTCCCAAGCC GGCCATTGAA AACTCAAGAG CTCCGGGACC CAGTGTTGTC AGGAGCCATT 
GCAACAAAGA TGGCCCGTTT CCATGGTATG GAGATGCCCT TCACCAAGGA GCCCCGCTGG 
TTGTTTGGGA CCATGGAGCG GTACCTAAAG CAGATCCAGG ACCTGCCGTC CACTAGCCTT 
CCCCAGATGA ACCTGGTGGA GATGTACAGC CTCAAGGATG AGATGAATCA CCTCAGGACG 
TTGCTAGACG CTACACCGTC CCCAGTGGTC TTCTGCCACA ATGACATCCA GGAAGGAAAC 
ATCTTACTGC TCTCAGAGCC AGACAGTGAT GACAACCTCA TGTTGGTTGA TTTCGAGTAC 
AGTAGTTACA ACTACAGGGG CTTTGACATT GGGAATCATT TCTGTGAGTG GGTTTACGAT 
TACACTTACG AGGAGTGGCC TTTCTACAAA GCAAGACCTG CAGACTACCC CACTAGAGAA 
CAGCAGCTCC TTTTCATCCG TCATTATCTG GCGGAGGTTC AGAAAGGTGA GGTCCTCTCC 
GAAGAGGAGC AGAAGAAACA GGAAGAAGAT TTGCTGATAG AGATCAGCCG GTATGCCCTG 
GCCTCTCATT TCTTCTGGGG CCTATGGTCC ACCCTCCAGG CTTCCATGTC CACTATAGAG 
TTTGGCTACT TGGAATACGC CCAATCTCGG TTCCAGTTCT ACTTCCAGCA GAAGGGGCAG 
CTGACCAGCT TCCTATCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763899.1
CDS	904..2127
Translation	MAADGTGVVGGGAVGGPLSKDGLLDAKCPEPIPNRRRSSSLSRDAQRRAYQWCREYLGGAWRRARPEELSVCPVRSGVSLTLLRVRLPGGLSNLLFRCSLPNHVPSMGGEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYLPSRPLKTQELRDPVLSGAIATKMARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQMNLVEMYSLKDEMNHLRTLLDATPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTYEEWPFYKARPADYPTREQQLLFIRHYLAEVQKGEVLSEEEQKKQEEDLLIEISRYALASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSFLSP

Target ORF information:

RefSeq Version	XM_008765677.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus choline kinase beta (Chkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765677.2

ORF Insert Sequence:

ATGGCGGCGG ATGGGACAGG TGTAGTCGGA GGAGGGGCTG TCGGCGGCCC CCTGTCCAAG 
GACGGTTTGC TGGATGCTAA GTGCCCAGAA CCAATCCCCA ATCGGCGGCG CTCTTCCTCG 
CTGTCCCGTG ACGCGCAGCG CCGAGCCTAT CAGTGGTGCC GGGAGTACCT GGGCGGAGCC 
TGGCGCAGAG CGCGGCCGGA GGAGCTGAGC GTTTGCCCCG TGAGGTCAGG GGTCAGCCTC 
ACACTCCTCC GGGTTCGGTT GCCCGGAGGC CTCAGCAACC TGCTCTTCCG ATGCTCGCTA 
CCGAACCACG TGCCCAGTAT GGGCGGGGAG CCCCGGGAGG TGCTGCTACG GCTGTACGGG 
GCTATCTTGC AGGGTGTAGA CTCCTTGGTA TTAGAAAGCG TGATGTTCGC CATTCTTGCA 
GAGAGATCTC TAGGGCCCCA ACTTTATGGA GTGTTTCCAG AGGGCCGCTT GGAACAGTAC 
CTCCCAAGCC GGCCATTGAA AACTCAAGAG CTCCGGGACC CAGTGTTGTC AGGAGCCATT 
GCAACAAAGA TGGCCCGTTT CCATGGTATG GAGATGCCCT TCACCAAGGA GCCCCGCTGG 
TTGTTTGGGA CCATGGAGCG GTACCTAAAG CAGATCCAGG ACCTGCCGTC CACTAGCCTT 
CCCCAGATGA ACCTGGTGGA GATGTACAGC CTCAAGGATG AGATGAATCA CCTCAGGACG 
TTGCTAGACG CTACACCGTC CCCAGTGGTC TTCTGCCACA ATGACATCCA GGAAGGAAAC 
ATCTTACTGC TCTCAGAGCC AGACAGTGAT GACAACCTCA TGTTGGTTGA TTTCGAGTAC 
AGTAGTTACA ACTACAGGGG CTTTGACATT GGGAATCATT TCTGTGAGTG GGTTTACGAT 
TACACTTACG AGGAGTGGCC TTTCTACAAA GCAAGACCTG CAGACTACCC CACTAGAGAA 
CAGCAGCTCC TTTTCATCCG TCATTATCTG GCGGAGGTTC AGAAAGGTGA GGTCCTCTCC 
GAAGAGGAGC AGAAGAAACA GGAAGAAGAT TTGCTGATAG AGATCAGCCG GTATGCCCTG 
GCCTCTCATT TCTTCTGGGG CCTATGGTCC ACCCTCCAGG CTTCCATGTC CACTATAGAG 
TTTGGCTACT TGGAATACGC CCAATCTCGG TTCCAGTTCT ACTTCCAGCA GAAGGGGCAG 
CTGACCAGCT TCCTATCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy.
Human molecular genetics20(19)3841-51(2011 Oct)
Mitsuhashi S,Hatakeyama H,Karahashi M,Koumura T,Nonaka I,Hayashi YK,Noguchi S,Sher RB,Nakagawa Y,Manfredi G,Goto Y,Cox GA,Nishino I

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis.
American journal of human genetics88(6)845-851(2011 Jun)
Mitsuhashi S,Ohkuma A,Talim B,Karahashi M,Koumura T,Aoyama C,Kurihara M,Quinlivan R,Sewry C,Mitsuhashi H,Goto K,Koksal B,Kale G,Ikeda K,Taguchi R,Noguchi S,Hayashi YK,Nonaka I,Sher RB,Sugimoto H,Nakagawa Y,Cox GA,Topaloglu H,Nishino I

Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment.
PloS one4(11)e7819(2009 Nov)
Gallego-Ortega D,Ramirez de Molina A,Ramos MA,Valdes-Mora F,Barderas MG,Sarmentero-Estrada J,Lacal JC

Polymorphism located between CPT1B and CHKB, and HLA-DRB1*1501-DQB1*0602 haplotype confer susceptibility to CNS hypersomnias (essential hypersomnia).
PloS one4(4)e5394(2009)
Miyagawa T,Honda M,Kawashima M,Shimada M,Tanaka S,Honda Y,Tokunaga K

Variant between CPT1B and CHKB associated with susceptibility to narcolepsy.
Nature genetics40(11)1324-8(2008 Nov)
Miyagawa T,Kawashima M,Nishida N,Ohashi J,Kimura R,Fujimoto A,Shimada M,Morishita S,Shigeta T,Lin L,Hong SC,Faraco J,Shin YK,Jeong JH,Okazaki Y,Tsuji S,Honda M,Honda Y,Mignot E,Tokunaga K

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Chkb cDNA ORF clone, Rattus norvegicus(Norway rat)

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