Edem3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edem3
Entrez Gene ID	289085
Full Name	ER degradation enhancing alpha-mannosidase like protein 3
Synonyms	RGD1561496
General protein information	Preferred Names ER degradation enhancing alpha-mannosidase like protein 3 Names ER degradation-enhancing alpha-mannosidase-like protein 3 ER degradation enhancer, mannosidase alpha-like 3 ER degradation-enhancing alpha-mannosidase-like 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249986.2	XP_006250048.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
NM_001191671.1	NP_001178600.1	ER degradation-enhancing alpha-mannosidase-like 3
XM_017598702.1	XP_017454191.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
XM_006249987.2	XP_006250049.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
XM_006249985.2	XP_006250047.1	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Edem3	NP_001034733.2
Rattus norvegicus (Norway rat)	Edem3	NP_001178600.1
Danio rerio (zebrafish)	edem3	XP_005166989.1
Pan troglodytes (chimpanzee)	EDEM3	XP_003308683.1
Caenorhabditis elegans (roundworm)	ZC506.1	NP_509671.3
Xenopus tropicalis (tropical clawed frog)	edem3	XP_002932190.2
Homo sapiens (human)	EDEM3	NP_079467.3
Macaca mulatta (Rhesus monkey)	EDEM3	XP_002802060.1
Canis lupus familiaris (dog)	EDEM3	XP_005622487.1
Bos taurus (cattle)	EDEM3	NP_001192782.1
Gallus gallus (chicken)	EDEM3	XP_422293.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008749	XP_314865.3
Drosophila melanogaster (fruit fly)	Edem2	NP_609611.1

Gene Ontology

The following Edem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa25621	NM_001191671.1 Latest version!	Rattus norvegicus ER degradation enhancer, mannosidase alpha-like 3 (Edem3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa44750	XM_006249985.2 Latest version!	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa44751	XM_006249986.2 Latest version!	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa44752	XM_006249987.2 Latest version!	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa58012	XM_017598702.1 Latest version!	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edem3 ( NM_001191671.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178600.1 Rattus norvegicus ER degradation enhancer, mannosidase alpha-like 3 (Edem3), mRNA.

Price & Availability↑

CloneID	ORa25621
Clone ID Related Accession (Same CDS sequence)	NM_001191671.1
Accession Version	NM_001191671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1279296000000
Organism	Rattus norvegicus(Norway rat)
Product	ER degradation-enhancing alpha-mannosidase-like 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR03085090.1 and AABR03085537.1. On Jul 17, 2010 this sequence version replaced XM_222721.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGATGGGCG AGTCCAGCTG GTCCAACATG CAATCCAAGC TGCGAGTTCA 
GTTGATGCTG AGGACGGGTT GCGGTTCATG CAGGAGATGA TAGAGTTGTC CAGTCAGCAA 
CAGAAGGAGC AGCAGCTGCC TCCTCGAGCT GTACAGATTA TCTCCCACCC ATTTTTTGGC 
AGGGTAGTCT TAACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACACAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TATAATGGCT GTTCGGAGCT CACTAACCCT 
GAGGCAGTGA TGGGAAAAAT TGCCTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCTCGAAACA TCCAGAATGC TGGCGCCATC GGTGGCATTG TGATTGATGA CAATGAGGGG 
AGCAGCAGTG ACACAGCCCC CCTGTTCCAG ATGGCTGGCG ACGGGAAAGA CACAGACGAC 
ATCAAGATCC CCATGCTGTT CCTGTTCAGC AAGGAAGGCA GCATCATCTT GGACGCCATC 
CGTGAGCACG AGCAGGTGGA GGTGCTCCTT TCTGACAAAG CGAGAGACCG AGATCCTGAG 
GTGGAAAATG AAGACCAGCC GTCTTCTGAA AACGACTCTC AGAATCAAAG TGCAGAGCAG 
ATGCTGCCAC TTCCTCAGAC AGTCGGCTTG GCTGATCAGG AGAGCCCTGG AAACCCTCTG 
GAGTCTCCCT CAGAGCCACC ATCGCCAGCA GACAGTGAAG AGGCTGCGGG CTTTGCGCCT 
TCTGAACAGA TCTCTAGCCC CACAGAGACC CAGGAGACGG CAGGTCTTGA TGGTGAGTGC 
ACAGACTTAG ATAACCAGCT TCAAGATCAA CCCGAAACCG AGGAAGACCC CAGTCCGAAC 
GTCAGCTGGG GTACGAAGGC CCAGCCTATG GACTCCATAC TAGCAGACTG GAATGAAGAC 
ATAGAAGCAT TTGAAATGAT GGAGAAGGAC GAACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178600.1
CDS	119..2917
Translation	MSEAGGGRGCGCRVPQRASWSLVAATAALYLMLATTVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATVFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNRNISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSVDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREHEQVEVLLSDKARDRDPEVENEDQPSSENDSQNQSAEQMLPLPQTVGLADQESPGNPLESPSEPPSPADSEEAAGFAPSEQISSPTETQETAGLDGECTDLDNQLQDQPETEEDPSPNVSWGTKAQPMDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	NM_001191671.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER degradation enhancer, mannosidase alpha-like 3 (Edem3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191671.1

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGATGGGCG AGTCCAGCTG GTCCAACATG CAATCCAAGC TGCGAGTTCA 
GTTGATGCTG AGGACGGGTT GCGGTTCATG CAGGAGATGA TAGAGTTGTC CAGTCAGCAA 
CAGAAGGAGC AGCAGCTGCC TCCTCGAGCT GTACAGATTA TCTCCCACCC ATTTTTTGGC 
AGGGTAGTCT TAACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACACAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TATAATGGCT GTTCGGAGCT CACTAACCCT 
GAGGCAGTGA TGGGAAAAAT TGCCTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCTCGAAACA TCCAGAATGC TGGCGCCATC GGTGGCATTG TGATTGATGA CAATGAGGGG 
AGCAGCAGTG ACACAGCCCC CCTGTTCCAG ATGGCTGGCG ACGGGAAAGA CACAGACGAC 
ATCAAGATCC CCATGCTGTT CCTGTTCAGC AAGGAAGGCA GCATCATCTT GGACGCCATC 
CGTGAGCACG AGCAGGTGGA GGTGCTCCTT TCTGACAAAG CGAGAGACCG AGATCCTGAG 
GTGGAAAATG AAGACCAGCC GTCTTCTGAA AACGACTCTC AGAATCAAAG TGCAGAGCAG 
ATGCTGCCAC TTCCTCAGAC AGTCGGCTTG GCTGATCAGG AGAGCCCTGG AAACCCTCTG 
GAGTCTCCCT CAGAGCCACC ATCGCCAGCA GACAGTGAAG AGGCTGCGGG CTTTGCGCCT 
TCTGAACAGA TCTCTAGCCC CACAGAGACC CAGGAGACGG CAGGTCTTGA TGGTGAGTGC 
ACAGACTTAG ATAACCAGCT TCAAGATCAA CCCGAAACCG AGGAAGACCC CAGTCCGAAC 
GTCAGCTGGG GTACGAAGGC CCAGCCTATG GACTCCATAC TAGCAGACTG GAATGAAGAC 
ATAGAAGCAT TTGAAATGAT GGAGAAGGAC GAACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem3 ( XM_006249985.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250047.1 Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44750
Clone ID Related Accession (Same CDS sequence)	XM_006249985.2
Accession Version	XM_006249985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGAAGTGGAG CTAAGCCTCC TCTGAGAGCC 
AGAGATTTTA TGGCCACTAA CCCTGAACAT TTAGAAATCC TGAAGAAGAT GGGGGTGAGT 
TTGATTCACC TCAAAGATGG GCGAGTCCAG CTGGTCCAAC ATGCAATCCA AGCTGCGAGT 
TCAGTTGATG CTGAGGACGG GTTGCGGTTC ATGCAGGAGA TGATAGAGTT GTCCAGTCAG 
CAACAGAAGG AGCAGCAGCT GCCTCCTCGA GCTGTACAGA TTATCTCCCA CCCATTTTTT 
GGCAGGGTAG TCTTAACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACACAAA 
GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATATAATG GCTGTTCGGA GCTCACTAAC 
CCTGAGGCAG TGATGGGAAA AATTGCCTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA 
AAGGCTCGAA ACATCCAGAA TGCTGGCGCC ATCGGTGGCA TTGTGATTGA TGACAATGAG 
GGGAGCAGCA GTGACACAGC CCCCCTGTTC CAGATGGCTG GCGACGGGAA AGACACAGAC 
GACATCAAGA TCCCCATGCT GTTCCTGTTC AGCAAGGAAG GCAGCATCAT CTTGGACGCC 
ATCCGTGAGC ACGAGCAGGT GGAGGTGCTC CTTTCTGACA AAGCGAGAGA CCGAGATCCT 
GAGGTGGAAA ATGAAGACNN NNNNNNNNNN NNNNNNGACT CTCAGAATCA AAGTGCAGAG 
CAGATGCTGC CACTTCCTCA GACAGTCGGC TTGGCTGATC AGGAGAGCCC TGGAAACCCT 
CTGGAGTCTC CCTCAGAGCC ACCATCGCCA GCAGACAGTG AAGAGGCTGC GGGCTTTGCG 
CCTTCTGAAC AGATCTCTAG CCCCACAGAG ACCCAGGAGA CGGCAGGTCT TGATGGTGAG 
TGCACAGACT TAGATAACCA GCTTCAAGAT CAACCCGAAA CCGAGGAAGA CCCCAGTCCG 
AACGTCAGCT GGGGTACGAA GGCCCAGCCT ATGGACTCCA TACTAGCAGA CTGGAATGAA 
GACATAGAAG CATTTGAAAT GATGGAGAAG GACGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250047.1
CDS	553..3354
Translation	MSEAGGGRGCGCRVPQRASWSLVAATAALYLMLATTVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATVFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNRNISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVREESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSVDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREHEQVEVLLSDKARDRDPEVENEDnullnullnullnullnullnullDSQNQSAEQMLPLPQTVGLADQESPGNPLESPSEPPSPADSEEAAGFAPSEQISSPTETQETAGLDGECTDLDNQLQDQPETEEDPSPNVSWGTKAQPMDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_006249985.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249985.2

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGAAGTGGAG CTAAGCCTCC TCTGAGAGCC 
AGAGATTTTA TGGCCACTAA CCCTGAACAT TTAGAAATCC TGAAGAAGAT GGGGGTGAGT 
TTGATTCACC TCAAAGATGG GCGAGTCCAG CTGGTCCAAC ATGCAATCCA AGCTGCGAGT 
TCAGTTGATG CTGAGGACGG GTTGCGGTTC ATGCAGGAGA TGATAGAGTT GTCCAGTCAG 
CAACAGAAGG AGCAGCAGCT GCCTCCTCGA GCTGTACAGA TTATCTCCCA CCCATTTTTT 
GGCAGGGTAG TCTTAACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACACAAA 
GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATATAATG GCTGTTCGGA GCTCACTAAC 
CCTGAGGCAG TGATGGGAAA AATTGCCTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA 
AAGGCTCGAA ACATCCAGAA TGCTGGCGCC ATCGGTGGCA TTGTGATTGA TGACAATGAG 
GGGAGCAGCA GTGACACAGC CCCCCTGTTC CAGATGGCTG GCGACGGGAA AGACACAGAC 
GACATCAAGA TCCCCATGCT GTTCCTGTTC AGCAAGGAAG GCAGCATCAT CTTGGACGCC 
ATCCGTGAGC ACGAGCAGGT GGAGGTGCTC CTTTCTGACA AAGCGAGAGA CCGAGATCCT 
GAGGTGGAAA ATGAAGACNN NNNNNNNNNN NNNNNNGACT CTCAGAATCA AAGTGCAGAG 
CAGATGCTGC CACTTCCTCA GACAGTCGGC TTGGCTGATC AGGAGAGCCC TGGAAACCCT 
CTGGAGTCTC CCTCAGAGCC ACCATCGCCA GCAGACAGTG AAGAGGCTGC GGGCTTTGCG 
CCTTCTGAAC AGATCTCTAG CCCCACAGAG ACCCAGGAGA CGGCAGGTCT TGATGGTGAG 
TGCACAGACT TAGATAACCA GCTTCAAGAT CAACCCGAAA CCGAGGAAGA CCCCAGTCCG 
AACGTCAGCT GGGGTACGAA GGCCCAGCCT ATGGACTCCA TACTAGCAGA CTGGAATGAA 
GACATAGAAG CATTTGAAAT GATGGAGAAG GACGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem3 ( XM_006249986.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250048.1 Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44751
Clone ID Related Accession (Same CDS sequence)	XM_006249986.2
Accession Version	XM_006249986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGAAGTGGAG CTAAGCCTCC TCTGAGAGCC 
AGAGATTTTA TGGCCACTAA CCCTGAACAT TTAGAAATCC TGAAGAAGAT GGGGGCTGCG 
AGTTCAGTTG ATGCTGAGGA CGGGTTGCGG TTCATGCAGG AGATGATAGA GTTGTCCAGT 
CAGCAACAGA AGGAGCAGCA GCTGCCTCCT CGAGCTGTAC AGATTATCTC CCACCCATTT 
TTTGGCAGGG TAGTCTTAAC TGCTGGACCA GCTCAGTTTG GGCTGGATCT GTCTAAACAC 
AAAGAGACAA GAGGATTTGT TGCAAGCAGT AAACCATATA ATGGCTGTTC GGAGCTCACT 
AACCCTGAGG CAGTGATGGG AAAAATTGCC TTGATACAAA GAGGACAGTG CATGTTTGCA 
GAAAAGGCTC GAAACATCCA GAATGCTGGC GCCATCGGTG GCATTGTGAT TGATGACAAT 
GAGGGGAGCA GCAGTGACAC AGCCCCCCTG TTCCAGATGG CTGGCGACGG GAAAGACACA 
GACGACATCA AGATCCCCAT GCTGTTCCTG TTCAGCAAGG AAGGCAGCAT CATCTTGGAC 
GCCATCCGTG AGCACGAGCA GGTGGAGGTG CTCCTTTCTG ACAAAGCGAG AGACCGAGAT 
CCTGAGGTGG AAAATGAAGA CNNNNNNNNN NNNNNNNNNG ACTCTCAGAA TCAAAGTGCA 
GAGCAGATGC TGCCACTTCC TCAGACAGTC GGCTTGGCTG ATCAGGAGAG CCCTGGAAAC 
CCTCTGGAGT CTCCCTCAGA GCCACCATCG CCAGCAGACA GTGAAGAGGC TGCGGGCTTT 
GCGCCTTCTG AACAGATCTC TAGCCCCACA GAGACCCAGG AGACGGCAGG TCTTGATGGT 
GAGTGCACAG ACTTAGATAA CCAGCTTCAA GATCAACCCG AAACCGAGGA AGACCCCAGT 
CCGAACGTCA GCTGGGGTAC GAAGGCCCAG CCTATGGACT CCATACTAGC AGACTGGAAT 
GAAGACATAG AAGCATTTGA AATGATGGAG AAGGACGAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250048.1
CDS	553..3297
Translation	MSEAGGGRGCGCRVPQRASWSLVAATAALYLMLATTVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATVFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNRNISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVREESFRSGAKPPLRARDFMATNPEHLEILKKMGAASSVDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREHEQVEVLLSDKARDRDPEVENEDnullnullnullnullnullnullDSQNQSAEQMLPLPQTVGLADQESPGNPLESPSEPPSPADSEEAAGFAPSEQISSPTETQETAGLDGECTDLDNQLQDQPETEEDPSPNVSWGTKAQPMDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_006249986.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249986.2

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGAAGTGGAG CTAAGCCTCC TCTGAGAGCC 
AGAGATTTTA TGGCCACTAA CCCTGAACAT TTAGAAATCC TGAAGAAGAT GGGGGCTGCG 
AGTTCAGTTG ATGCTGAGGA CGGGTTGCGG TTCATGCAGG AGATGATAGA GTTGTCCAGT 
CAGCAACAGA AGGAGCAGCA GCTGCCTCCT CGAGCTGTAC AGATTATCTC CCACCCATTT 
TTTGGCAGGG TAGTCTTAAC TGCTGGACCA GCTCAGTTTG GGCTGGATCT GTCTAAACAC 
AAAGAGACAA GAGGATTTGT TGCAAGCAGT AAACCATATA ATGGCTGTTC GGAGCTCACT 
AACCCTGAGG CAGTGATGGG AAAAATTGCC TTGATACAAA GAGGACAGTG CATGTTTGCA 
GAAAAGGCTC GAAACATCCA GAATGCTGGC GCCATCGGTG GCATTGTGAT TGATGACAAT 
GAGGGGAGCA GCAGTGACAC AGCCCCCCTG TTCCAGATGG CTGGCGACGG GAAAGACACA 
GACGACATCA AGATCCCCAT GCTGTTCCTG TTCAGCAAGG AAGGCAGCAT CATCTTGGAC 
GCCATCCGTG AGCACGAGCA GGTGGAGGTG CTCCTTTCTG ACAAAGCGAG AGACCGAGAT 
CCTGAGGTGG AAAATGAAGA CNNNNNNNNN NNNNNNNNNG ACTCTCAGAA TCAAAGTGCA 
GAGCAGATGC TGCCACTTCC TCAGACAGTC GGCTTGGCTG ATCAGGAGAG CCCTGGAAAC 
CCTCTGGAGT CTCCCTCAGA GCCACCATCG CCAGCAGACA GTGAAGAGGC TGCGGGCTTT 
GCGCCTTCTG AACAGATCTC TAGCCCCACA GAGACCCAGG AGACGGCAGG TCTTGATGGT 
GAGTGCACAG ACTTAGATAA CCAGCTTCAA GATCAACCCG AAACCGAGGA AGACCCCAGT 
CCGAACGTCA GCTGGGGTAC GAAGGCCCAG CCTATGGACT CCATACTAGC AGACTGGAAT 
GAAGACATAG AAGCATTTGA AATGATGGAG AAGGACGAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem3 ( XM_006249987.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250049.1 Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa44752
Clone ID Related Accession (Same CDS sequence)	XM_006249987.2
Accession Version	XM_006249987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGCTGCGAGT 
TCAGTTGATG CTGAGGACGG GTTGCGGTTC ATGCAGGAGA TGATAGAGTT GTCCAGTCAG 
CAACAGAAGG AGCAGCAGCT GCCTCCTCGA GCTGTACAGA TTATCTCCCA CCCATTTTTT 
GGCAGGGTAG TCTTAACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACACAAA 
GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATATAATG GCTGTTCGGA GCTCACTAAC 
CCTGAGGCAG TGATGGGAAA AATTGCCTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA 
AAGGCTCGAA ACATCCAGAA TGCTGGCGCC ATCGGTGGCA TTGTGATTGA TGACAATGAG 
GGGAGCAGCA GTGACACAGC CCCCCTGTTC CAGATGGCTG GCGACGGGAA AGACACAGAC 
GACATCAAGA TCCCCATGCT GTTCCTGTTC AGCAAGGAAG GCAGCATCAT CTTGGACGCC 
ATCCGTGAGC ACGAGCAGGT GGAGGTGCTC CTTTCTGACA AAGCGAGAGA CCGAGATCCT 
GAGGTGGAAA ATGAAGACNN NNNNNNNNNN NNNNNNGACT CTCAGAATCA AAGTGCAGAG 
CAGATGCTGC CACTTCCTCA GACAGTCGGC TTGGCTGATC AGGAGAGCCC TGGAAACCCT 
CTGGAGTCTC CCTCAGAGCC ACCATCGCCA GCAGACAGTG AAGAGGCTGC GGGCTTTGCG 
CCTTCTGAAC AGATCTCTAG CCCCACAGAG ACCCAGGAGA CGGCAGGTCT TGATGGTGAG 
TGCACAGACT TAGATAACCA GCTTCAAGAT CAACCCGAAA CCGAGGAAGA CCCCAGTCCG 
AACGTCAGCT GGGGTACGAA GGCCCAGCCT ATGGACTCCA TACTAGCAGA CTGGAATGAA 
GACATAGAAG CATTTGAAAT GATGGAGAAG GACGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250049.1
CDS	553..3294
Translation	MSEAGGGRGCGCRVPQRASWSLVAATAALYLMLATTVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATVFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNRNISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGAASSVDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREHEQVEVLLSDKARDRDPEVENEDnullnullnullnullnullnullDSQNQSAEQMLPLPQTVGLADQESPGNPLESPSEPPSPADSEEAAGFAPSEQISSPTETQETAGLDGECTDLDNQLQDQPETEEDPSPNVSWGTKAQPMDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_006249987.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249987.2

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGCTGCGAGT 
TCAGTTGATG CTGAGGACGG GTTGCGGTTC ATGCAGGAGA TGATAGAGTT GTCCAGTCAG 
CAACAGAAGG AGCAGCAGCT GCCTCCTCGA GCTGTACAGA TTATCTCCCA CCCATTTTTT 
GGCAGGGTAG TCTTAACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACACAAA 
GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATATAATG GCTGTTCGGA GCTCACTAAC 
CCTGAGGCAG TGATGGGAAA AATTGCCTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA 
AAGGCTCGAA ACATCCAGAA TGCTGGCGCC ATCGGTGGCA TTGTGATTGA TGACAATGAG 
GGGAGCAGCA GTGACACAGC CCCCCTGTTC CAGATGGCTG GCGACGGGAA AGACACAGAC 
GACATCAAGA TCCCCATGCT GTTCCTGTTC AGCAAGGAAG GCAGCATCAT CTTGGACGCC 
ATCCGTGAGC ACGAGCAGGT GGAGGTGCTC CTTTCTGACA AAGCGAGAGA CCGAGATCCT 
GAGGTGGAAA ATGAAGACNN NNNNNNNNNN NNNNNNGACT CTCAGAATCA AAGTGCAGAG 
CAGATGCTGC CACTTCCTCA GACAGTCGGC TTGGCTGATC AGGAGAGCCC TGGAAACCCT 
CTGGAGTCTC CCTCAGAGCC ACCATCGCCA GCAGACAGTG AAGAGGCTGC GGGCTTTGCG 
CCTTCTGAAC AGATCTCTAG CCCCACAGAG ACCCAGGAGA CGGCAGGTCT TGATGGTGAG 
TGCACAGACT TAGATAACCA GCTTCAAGAT CAACCCGAAA CCGAGGAAGA CCCCAGTCCG 
AACGTCAGCT GGGGTACGAA GGCCCAGCCT ATGGACTCCA TACTAGCAGA CTGGAATGAA 
GACATAGAAG CATTTGAAAT GATGGAGAAG GACGAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem3 ( XM_017598702.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454191.1 Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X1, mRNA.

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CloneID	ORa58012
Clone ID Related Accession (Same CDS sequence)	XM_017598702.1
Accession Version	XM_017598702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGATGGGCG AGTCCAGCTG GTCCAACATG CAATCCAAGC TGCGAGTTCA 
GTTGATGCTG AGGACGGGTT GCGGTTCATG CAGGAGATGA TAGAGTTGTC CAGTCAGCAA 
CAGAAGGAGC AGCAGCTGCC TCCTCGAGCT GTACAGATTA TCTCCCACCC ATTTTTTGGC 
AGGGTAGTCT TAACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACACAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TATAATGGCT GTTCGGAGCT CACTAACCCT 
GAGGCAGTGA TGGGAAAAAT TGCCTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCTCGAAACA TCCAGAATGC TGGCGCCATC GGTGGCATTG TGATTGATGA CAATGAGGGG 
AGCAGCAGTG ACACAGCCCC CCTGTTCCAG ATGGCTGGCG ACGGGAAAGA CACAGACGAC 
ATCAAGATCC CCATGCTGTT CCTGTTCAGC AAGGAAGGCA GCATCATCTT GGACGCCATC 
CGTGAGCACG AGCAGGTGGA GGTGCTCCTT TCTGACAAAG CGAGAGACCG AGCAGCCATA 
TTAAAAGGTA AAAGGATTCC AAGCTACATT ATTAACAGTA ATCCTGAGGT GGAAAATGAA 
GACNNNNNNN NNNNNNNNNN NGACTCTCAG AATCAAAGTG CAGAGCAGAT GCTGCCACTT 
CCTCAGACAG TCGGCTTGGC TGATCAGGAG AGCCCTGGAA ACCCTCTGGA GTCTCCCTCA 
GAGCCACCAT CGCCAGCAGA CAGTGAAGAG GCTGCGGGCT TTGCGCCTTC TGAACAGATC 
TCTAGCCCCA CAGAGACCCA GGAGACGGCA GGTCTTGATG GTGAGTGCAC AGACTTAGAT 
AACCAGCTTC AAGATCAACC CGAAACCGAG GAAGACCCCA GTCCGAACGT CAGCTGGGGT 
ACGAAGGCCC AGCCTATGGA CTCCATACTA GCAGACTGGA ATGAAGACAT AGAAGCATTT 
GAAATGATGG AGAAGGACGA ACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454191.1
CDS	553..3399
Translation	MSEAGGGRGCGCRVPQRASWSLVAATAALYLMLATTVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATVFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNRNISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSVDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREHEQVEVLLSDKARDRAAILKGKRIPSYIINSNPEVENEDnullnullnullnullnullnullDSQNQSAEQMLPLPQTVGLADQESPGNPLESPSEPPSPADSEEAAGFAPSEQISSPTETQETAGLDGECTDLDNQLQDQPETEEDPSPNVSWGTKAQPMDSILADWNEDIEAFEMMEKDEL

Target ORF information:

RefSeq Version	XM_017598702.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER degradation enhancing alpha-mannosidase like protein 3 (Edem3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598702.1

ORF Insert Sequence:

ATGAGCGAAG CCGGCGGCGG CCGGGGCTGT GGGTGCCGGG TCCCCCAGCG AGCGTCATGG 
AGCCTGGTGG CAGCGACGGC CGCGCTCTAT CTGATGTTGG CCACGACCGT GTGCACGGCA 
GGGGCCGCGC CCATGAGTAG GGAAGAGAAG CAGAAGCTTG GAAATCAAGT CCTGGAAATG 
TTTGATCACG CTTACGGCAA CTATATGGAA CATGCCTACC CAGCTGATGA ACTCATGCCT 
TTAACCTGTC GAGGTCGTGT CAGAGGCCAG GAGCCAAGCC GGGGTGACGT GGATGATGCC 
TTGGGAAAGT TCTCCCTAAC ACTGATCGAT TCTTTGGACA CTCTTGTGGT ATTAAACAAA 
ACTAAGGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGGG ATGTTAATCT AGATAATGAT 
GTAGTTGTTT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC 
TCGCTGGCAA TCATGCTGAA GGAAAAAGGG GAATACATGC AGTGGTATAA CGACGAGCTT 
CTCCAAATGG CAAAGCAGCT GGGTTACAAG CTCCTTCCAG CTTTCAACAC TACCAGTGGC 
CTTCCATATC CCAGGATTAA TTTAAAGTTT GGCATCAGAA AACCGGAAGC CCGGACGGGA 
ACTGAGACGG ACACCTGCAC GGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCATTG 
AGTCGGTTCA CAGGAGCTAC AGTATTCGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC 
TGGGAGAAAA GACAGCGAAG TAGCAATTTA GTAGGCGTGA CTATCAATAT TCATACTGGA 
GACTGGGTGC GCAAAGACAG TGGAGTTGGA GCAGGGATCG ACTCATATTA TGAGTATCTG 
TTGAAAGCCT ATGTCTTGCT CGGAGATGAC AGTTTTCTGG AAAGATTTAA TACACATTAT 
GATGCCATAA TGAGGTATAT TAGCCAGCCC CCTCTCCTGC TCGATGTGCA TATCCACAAA 
CCAATGCTCA ATGCCCGGAC CTGGATGGAC GCACTGCTTG CCTTCTTTCC AGGTTTGCAG 
GTTTTAAAGG GTGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA CCAGGTGATT 
AAAAAACACA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTTCA CTGGGCTCAG 
CACCCTCTAA GGCCAGAGTT TGCAGAGAGT ACCTACTTCT TATATAAGGC TACAGGAGAC 
CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TGAATAAATA CGCTCGAGTG 
CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGCACTGGGA GTCATGAGGA CAGAATGGAT 
TCGTTCTTCT TGGCTGAAAT GTTCAAATAT CTGTACCTGT TATTTGCTGA TAAAGAAGAC 
ATTATTTTTG ACATAGAAGA CTATATCTTT ACAACAGAGG CTCATCTGTT ACCTCTTTGG 
CTTTCTACCA CAAACCGAAA CATCTCGAAG AAGAACACAA CCTCAGAATA CACAGAACTG 
GATGATAGTA ACTTTGATTG GACTTGTCCA AACACGCAGA TTCTTTTTCC TAATGATCCA 
TTGTATGCCC AGAGCATCCG TGAACCCTTG AAAAACGTGG TGGATAAGAG CTGTCCAAGA 
GGCATCATCA GAGTAGAGGA GAGTTTCAGA AGTGGAGCTA AGCCTCCTCT GAGAGCCAGA 
GATTTTATGG CCACTAACCC TGAACATTTA GAAATCCTGA AGAAGATGGG GGTGAGTTTG 
ATTCACCTCA AAGATGGGCG AGTCCAGCTG GTCCAACATG CAATCCAAGC TGCGAGTTCA 
GTTGATGCTG AGGACGGGTT GCGGTTCATG CAGGAGATGA TAGAGTTGTC CAGTCAGCAA 
CAGAAGGAGC AGCAGCTGCC TCCTCGAGCT GTACAGATTA TCTCCCACCC ATTTTTTGGC 
AGGGTAGTCT TAACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACACAAAGAG 
ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TATAATGGCT GTTCGGAGCT CACTAACCCT 
GAGGCAGTGA TGGGAAAAAT TGCCTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG 
GCTCGAAACA TCCAGAATGC TGGCGCCATC GGTGGCATTG TGATTGATGA CAATGAGGGG 
AGCAGCAGTG ACACAGCCCC CCTGTTCCAG ATGGCTGGCG ACGGGAAAGA CACAGACGAC 
ATCAAGATCC CCATGCTGTT CCTGTTCAGC AAGGAAGGCA GCATCATCTT GGACGCCATC 
CGTGAGCACG AGCAGGTGGA GGTGCTCCTT TCTGACAAAG CGAGAGACCG AGCAGCCATA 
TTAAAAGGTA AAAGGATTCC AAGCTACATT ATTAACAGTA ATCCTGAGGT GGAAAATGAA 
GACNNNNNNN NNNNNNNNNN NGACTCTCAG AATCAAAGTG CAGAGCAGAT GCTGCCACTT 
CCTCAGACAG TCGGCTTGGC TGATCAGGAG AGCCCTGGAA ACCCTCTGGA GTCTCCCTCA 
GAGCCACCAT CGCCAGCAGA CAGTGAAGAG GCTGCGGGCT TTGCGCCTTC TGAACAGATC 
TCTAGCCCCA CAGAGACCCA GGAGACGGCA GGTCTTGATG GTGAGTGCAC AGACTTAGAT 
AACCAGCTTC AAGATCAACC CGAAACCGAG GAAGACCCCA GTCCGAACGT CAGCTGGGGT 
ACGAAGGCCC AGCCTATGGA CTCCATACTA GCAGACTGGA ATGAAGACAT AGAAGCATTT 
GAAATGATGG AGAAGGACGA ACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Edem3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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