Hspa1l cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa1l
Entrez Gene ID	24963
Full Name	heat shock protein family A (Hsp70) member 1 like
Synonyms	Hsp70-3
General protein information	Preferred Names heat shock protein family A (Hsp70) member 1 like Names heat shock 70 kDa protein 1-like HSP70.3 Heat shock protein 70-1l heat shock 70 kDa protein 1L heat shock 70 kDa protein 3 heat shock protein 1-like heat shock protein 70
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	one of three major histocompatibility complex (MHC)-linked heat shock protein 70 (Hsp70) genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601544.1	XP_017457033.1	heat shock 70 kDa protein 1-like isoform X1
NM_212546.4	NP_997711.1	heat shock 70 kDa protein 1-like

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09602	XP_963366.1
Saccharomyces cerevisiae (baker's yeast)	SSA2	NP_013076.1
Danio rerio (zebrafish)	zgc:174006	XP_005163979.1
Eremothecium gossypii	AGOS_AFR114W	NP_985661.1
Canis lupus familiaris (dog)	HSPA1L	XP_005627220.1
Mus musculus (house mouse)	Hspa1l	NP_038586.2
Danio rerio (zebrafish)	si:ch211-199o1.2	NP_001093532.1
Danio rerio (zebrafish)	hsp70	NP_571472.1
Arabidopsis thaliana (thale cress)	Hsp70b	NP_173055.1
Magnaporthe oryzae (rice blast fungus)	MGG_06958	XP_003709704.1
Pan troglodytes (chimpanzee)	HSPA1L	XP_001160137.2
Rattus norvegicus (Norway rat)	Hspa1l	NP_997711.1
Saccharomyces cerevisiae (baker's yeast)	SSA1	NP_009396.2
Kluyveromyces lactis	KLLA0D04224g	XP_453252.1
Schizosaccharomyces pombe (fission yeast)	ssa1	NP_593704.1
Schizosaccharomyces pombe (fission yeast)	ssa2	NP_588421.1
Homo sapiens (human)	HSPA1L	NP_005518.3
Macaca mulatta (Rhesus monkey)	LOC716633	XP_002803717.1
Bos taurus (cattle)	HSPA1L	NP_001161367.1
Danio rerio (zebrafish)	hsp70l	NP_001107061.1
Danio rerio (zebrafish)	hsp70.2	XP_003198158.1

Gene Ontology
Bibliography

Related articles in PubMed

HLA class III genes involvement in Kawasaki disease: a case-control study in Caucasian population.
Maggioli E, Boiocchi C, Zorzetto M, Mannarino S, Bossi G, Cuccia M
International journal of immunogenetics41(1)44-53(2014 Feb)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics75(12)3574-84(2012 Jun)

Heat shock protein 70 gene polymorphisms in sudden sensorineural hearing loss.
Chien CY, Chang NC, Tai SY, Wang LF, Wu MT, Ho KY
Audiology & neuro-otology17(6)381-5(2012)

The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction.
Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B
The Journal of biological chemistry286(42)36875-87(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hspa1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05630	NM_212546.4 Latest version!	Rattus norvegicus heat shock protein family A (Hsp70) member 1 like (Hspa1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa05630	XM_017601544.1 Latest version!	Rattus norvegicus heat shock protein 1-like (Hspa1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa1l ( NM_212546.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997711.1 Rattus norvegicus heat shock protein family A (Hsp70) member 1 like (Hspa1l), mRNA.

Price & Availability↑

CloneID	ORa05630
Clone ID Related Accession (Same CDS sequence)	XM_017601544.1 , NM_212546.4
Accession Version	NM_212546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Rattus norvegicus(Norway rat)
Product	heat shock 70 kDa protein 1-like
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883045.1. On Oct 21, 2009 this sequence version replaced NM_212546.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108297.1, CK600407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689604 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTA ATAAAGGAAT GGCCATAGGC ATCGACCTGG GCACCACCTA CTCGTGCGTG 
GGCGTGTTCC AGCACGGCAA GGTGGAGATC ATCGCCAACG ACCAGGGCAA CCGCACGACC 
CCAAGCTACG TGGCCTTCAC CGACACCGAG CGGCTCATCG GGGACGCCGC CAAGAACCAG 
GTGGCCATGA ATCCCCAGAA CACTGTTTTT GACGCCAAAC GTCTAATTGG CAGGAAGTTT 
AATGATCCCG TCGTGCAGTC GGATATGAAG CTGTGGCCAT TTCAAGTGAT CAATGAAGCG 
GGCAAGCCTA AGGTGCTGGT GTCCTACAAA GGAGAGAAGA AAGCCTTCTA CCCGGAGGAG 
ATCTCGTCCA TGGTGCTGAC CAAGATGAAG GAGACTGCAG AGGCTTTTTT GGGCCACAGT 
GTCACCAACG CTGTGATCAC CGTGCCAGCC TATTTCAATG ACTCCCAGAG ACAGGCCACT 
AAAGATGCAG GGGTCATCGC AGGACTCAAC GTGCTGCGAA TCATCAATGA GCCCACAGCG 
GCGGCCATCG CCTACGGCTT GGATAAAGGA AGTCACGGAG AGCGGCACGT GCTCATCTTC 
GACCTGGGGG GCGGCACGTT CGACGTGTCC ATCCTGACGA TCGACGACGG CATCTTCGAG 
GTGAAGGCCA CGGCGGGCGA CACGCACCTG GGCGGGGAGG ACTTCGACAA CCGGCTGGTG 
AGCCACTTCG TGGAGGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGCCA GAACAAGCGC 
GCGGTGCGGC GTCTGCGCAC GGCGTGCGAG AGGGCCAAGA GGACGCTGTC GTCCAGCACC 
CAGGCCAACC TGGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACG 
CGGGCGCGGT TCGAGGAGCT GTGTGCAGAC CTATTTAGAG GCACGCTTGA GCCCGTGGAG 
AAGTCTCTCC GGGACGCCAA GATGGACAAG GCTAAAATCC ATGACATTGT TCTAGTAGGG 
GGCTCTACCC GCATCCCAAA GGTGCAAAAG CTGCTTCAAG ACTACTTTAA TGGACGGGAT 
CTCAACAAGA GTATCAATCC CGATGAGGCG GTGGCGTACG GAGCTGCAGT CCAGGCAGCT 
ATTTTAATGG GCGACAAATC TGAAAAAGTA CAGGATTTGC TTTTGTTGGA CGTAGCTCCC 
CTGTCTCTAG GATTGGAGAC AGCCGGGGGT GTGATGACTG TTCTGATCAA GCGCAACTCC 
ACCATCCCCA CCAAGCAGAC GCAGATCTTC ACCACCTACT CGGACAACCA GCCCGGGGTG 
CTGATCCAGG TGTACGAGGG CGAGAGGGCC ATGACGCGCG ACAACAACCT CCTGGGGCGC 
TTTGACCTGA CTGGAATACC CCCTGCACCT AGGGGTGTGC CCCAGATCGA GGTGACCTTC 
GACATCGACG CCAACGGGAT TCTCAACGTC ACAGCCATGG ACAAGAGCAC CGGCAAGGCC 
AACAAGATCA CCATCACCAA CGACAAGGGC CGCTTGAGCA AGGAGGAGAT CGAGCGCATG 
GTGCAAGAGG CCGAGCGGTA CAAGGCCGAG GACGAGGGCC AGAGGGAGAA GATCGCTGCC 
AAAAATGCCT TAGAATCGTA TGCCTTTAAC ATGAAGAGCG CTGTAGGCGA TGAGGGTCTG 
AAGGACAAGA TCAGCGAGTC TGATAAAAAG AAAATACTGG ATAAATGCAG TGAGGTCCTT 
TCCTGGCTGG AGGCCAACCA GCTGGCGGAG AAAGAAGAGT TCGATCATAA AAGAAAAGAG 
CTGGAAAACA TGTGCAACCC TATCATCACA AAGCTGTACC AGAGCGGCTG CACAGGGCCC 
ACCTGCGCGC CAGGGTATAC ACCCGGCAGG GCTGCCACAG GCCCCACCAT TGAGGAAGTA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997711.1
CDS	162..2087
Translation	MAANKGMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQSDMKLWPFQVINEAGKPKVLVSYKGEKKAFYPEEISSMVLTKMKETAEAFLGHSVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGSHGERHVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAMDKSTGKANKITITNDKGRLSKEEIERMVQEAERYKAEDEGQREKIAAKNALESYAFNMKSAVGDEGLKDKISESDKKKILDKCSEVLSWLEANQLAEKEEFDHKRKELENMCNPIITKLYQSGCTGPTCAPGYTPGRAATGPTIEEVD

Target ORF information:

RefSeq Version	NM_212546.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family A (Hsp70) member 1 like (Hspa1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212546.4

ORF Insert Sequence:

ATGGCTGCTA ATAAAGGAAT GGCCATAGGC ATCGACCTGG GCACCACCTA CTCGTGCGTG 
GGCGTGTTCC AGCACGGCAA GGTGGAGATC ATCGCCAACG ACCAGGGCAA CCGCACGACC 
CCAAGCTACG TGGCCTTCAC CGACACCGAG CGGCTCATCG GGGACGCCGC CAAGAACCAG 
GTGGCCATGA ATCCCCAGAA CACTGTTTTT GACGCCAAAC GTCTAATTGG CAGGAAGTTT 
AATGATCCCG TCGTGCAGTC GGATATGAAG CTGTGGCCAT TTCAAGTGAT CAATGAAGCG 
GGCAAGCCTA AGGTGCTGGT GTCCTACAAA GGAGAGAAGA AAGCCTTCTA CCCGGAGGAG 
ATCTCGTCCA TGGTGCTGAC CAAGATGAAG GAGACTGCAG AGGCTTTTTT GGGCCACAGT 
GTCACCAACG CTGTGATCAC CGTGCCAGCC TATTTCAATG ACTCCCAGAG ACAGGCCACT 
AAAGATGCAG GGGTCATCGC AGGACTCAAC GTGCTGCGAA TCATCAATGA GCCCACAGCG 
GCGGCCATCG CCTACGGCTT GGATAAAGGA AGTCACGGAG AGCGGCACGT GCTCATCTTC 
GACCTGGGGG GCGGCACGTT CGACGTGTCC ATCCTGACGA TCGACGACGG CATCTTCGAG 
GTGAAGGCCA CGGCGGGCGA CACGCACCTG GGCGGGGAGG ACTTCGACAA CCGGCTGGTG 
AGCCACTTCG TGGAGGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGCCA GAACAAGCGC 
GCGGTGCGGC GTCTGCGCAC GGCGTGCGAG AGGGCCAAGA GGACGCTGTC GTCCAGCACC 
CAGGCCAACC TGGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACG 
CGGGCGCGGT TCGAGGAGCT GTGTGCAGAC CTATTTAGAG GCACGCTTGA GCCCGTGGAG 
AAGTCTCTCC GGGACGCCAA GATGGACAAG GCTAAAATCC ATGACATTGT TCTAGTAGGG 
GGCTCTACCC GCATCCCAAA GGTGCAAAAG CTGCTTCAAG ACTACTTTAA TGGACGGGAT 
CTCAACAAGA GTATCAATCC CGATGAGGCG GTGGCGTACG GAGCTGCAGT CCAGGCAGCT 
ATTTTAATGG GCGACAAATC TGAAAAAGTA CAGGATTTGC TTTTGTTGGA CGTAGCTCCC 
CTGTCTCTAG GATTGGAGAC AGCCGGGGGT GTGATGACTG TTCTGATCAA GCGCAACTCC 
ACCATCCCCA CCAAGCAGAC GCAGATCTTC ACCACCTACT CGGACAACCA GCCCGGGGTG 
CTGATCCAGG TGTACGAGGG CGAGAGGGCC ATGACGCGCG ACAACAACCT CCTGGGGCGC 
TTTGACCTGA CTGGAATACC CCCTGCACCT AGGGGTGTGC CCCAGATCGA GGTGACCTTC 
GACATCGACG CCAACGGGAT TCTCAACGTC ACAGCCATGG ACAAGAGCAC CGGCAAGGCC 
AACAAGATCA CCATCACCAA CGACAAGGGC CGCTTGAGCA AGGAGGAGAT CGAGCGCATG 
GTGCAAGAGG CCGAGCGGTA CAAGGCCGAG GACGAGGGCC AGAGGGAGAA GATCGCTGCC 
AAAAATGCCT TAGAATCGTA TGCCTTTAAC ATGAAGAGCG CTGTAGGCGA TGAGGGTCTG 
AAGGACAAGA TCAGCGAGTC TGATAAAAAG AAAATACTGG ATAAATGCAG TGAGGTCCTT 
TCCTGGCTGG AGGCCAACCA GCTGGCGGAG AAAGAAGAGT TCGATCATAA AAGAAAAGAG 
CTGGAAAACA TGTGCAACCC TATCATCACA AAGCTGTACC AGAGCGGCTG CACAGGGCCC 
ACCTGCGCGC CAGGGTATAC ACCCGGCAGG GCTGCCACAG GCCCCACCAT TGAGGAAGTA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspa1l ( XM_017601544.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457033.1 Rattus norvegicus heat shock protein 1-like (Hspa1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05630
Clone ID Related Accession (Same CDS sequence)	XM_017601544.1 , NM_212546.4
Accession Version	XM_017601544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	heat shock 70 kDa protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTA ATAAAGGAAT GGCCATAGGC ATCGACCTGG GCACCACCTA CTCGTGCGTG 
GGCGTGTTCC AGCACGGCAA GGTGGAGATC ATCGCCAACG ACCAGGGCAA CCGCACGACC 
CCAAGCTACG TGGCCTTCAC CGACACCGAG CGGCTCATCG GGGACGCCGC CAAGAACCAG 
GTGGCCATGA ATCCCCAGAA CACTGTTTTT GACGCCAAAC GTCTAATTGG CAGGAAGTTT 
AATGATCCCG TCGTGCAGTC GGATATGAAG CTGTGGCCAT TTCAAGTGAT CAATGAAGCG 
GGCAAGCCTA AGGTGCTGGT GTCCTACAAA GGAGAGAAGA AAGCCTTCTA CCCGGAGGAG 
ATCTCGTCCA TGGTGCTGAC CAAGATGAAG GAGACTGCAG AGGCTTTTTT GGGCCACAGT 
GTCACCAACG CTGTGATCAC CGTGCCAGCC TATTTCAATG ACTCCCAGAG ACAGGCCACT 
AAAGATGCAG GGGTCATCGC AGGACTCAAC GTGCTGCGAA TCATCAATGA GCCCACAGCG 
GCGGCCATCG CCTACGGCTT GGATAAAGGA AGTCACGGAG AGCGGCACGT GCTCATCTTC 
GACCTGGGGG GCGGCACGTT CGACGTGTCC ATCCTGACGA TCGACGACGG CATCTTCGAG 
GTGAAGGCCA CGGCGGGCGA CACGCACCTG GGCGGGGAGG ACTTCGACAA CCGGCTGGTG 
AGCCACTTCG TGGAGGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGCCA GAACAAGCGC 
GCGGTGCGGC GTCTGCGCAC GGCGTGCGAG AGGGCCAAGA GGACGCTGTC GTCCAGCACC 
CAGGCCAACC TGGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACG 
CGGGCGCGGT TCGAGGAGCT GTGTGCAGAC CTATTTAGAG GCACGCTTGA GCCCGTGGAG 
AAGTCTCTCC GGGACGCCAA GATGGACAAG GCTAAAATCC ATGACATTGT TCTAGTAGGG 
GGCTCTACCC GCATCCCAAA GGTGCAAAAG CTGCTTCAAG ACTACTTTAA TGGACGGGAT 
CTCAACAAGA GTATCAATCC CGATGAGGCG GTGGCGTACG GAGCTGCAGT CCAGGCAGCT 
ATTTTAATGG GCGACAAATC TGAAAAAGTA CAGGATTTGC TTTTGTTGGA CGTAGCTCCC 
CTGTCTCTAG GATTGGAGAC AGCCGGGGGT GTGATGACTG TTCTGATCAA GCGCAACTCC 
ACCATCCCCA CCAAGCAGAC GCAGATCTTC ACCACCTACT CGGACAACCA GCCCGGGGTG 
CTGATCCAGG TGTACGAGGG CGAGAGGGCC ATGACGCGCG ACAACAACCT CCTGGGGCGC 
TTTGACCTGA CTGGAATACC CCCTGCACCT AGGGGTGTGC CCCAGATCGA GGTGACCTTC 
GACATCGACG CCAACGGGAT TCTCAACGTC ACAGCCATGG ACAAGAGCAC CGGCAAGGCC 
AACAAGATCA CCATCACCAA CGACAAGGGC CGCTTGAGCA AGGAGGAGAT CGAGCGCATG 
GTGCAAGAGG CCGAGCGGTA CAAGGCCGAG GACGAGGGCC AGAGGGAGAA GATCGCTGCC 
AAAAATGCCT TAGAATCGTA TGCCTTTAAC ATGAAGAGCG CTGTAGGCGA TGAGGGTCTG 
AAGGACAAGA TCAGCGAGTC TGATAAAAAG AAAATACTGG ATAAATGCAG TGAGGTCCTT 
TCCTGGCTGG AGGCCAACCA GCTGGCGGAG AAAGAAGAGT TCGATCATAA AAGAAAAGAG 
CTGGAAAACA TGTGCAACCC TATCATCACA AAGCTGTACC AGAGCGGCTG CACAGGGCCC 
ACCTGCGCGC CAGGGTATAC ACCCGGCAGG GCTGCCACAG GCCCCACCAT TGAGGAAGTA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457033.1
CDS	131..2056
Translation	MAANKGMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQSDMKLWPFQVINEAGKPKVLVSYKGEKKAFYPEEISSMVLTKMKETAEAFLGHSVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGSHGERHVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAKIHDIVLVGGSTRIPKVQKLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTVLIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAMDKSTGKANKITITNDKGRLSKEEIERMVQEAERYKAEDEGQREKIAAKNALESYAFNMKSAVGDEGLKDKISESDKKKILDKCSEVLSWLEANQLAEKEEFDHKRKELENMCNPIITKLYQSGCTGPTCAPGYTPGRAATGPTIEEVD

Target ORF information:

RefSeq Version	XM_017601544.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein 1-like (Hspa1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601544.1

ORF Insert Sequence:

ATGGCTGCTA ATAAAGGAAT GGCCATAGGC ATCGACCTGG GCACCACCTA CTCGTGCGTG 
GGCGTGTTCC AGCACGGCAA GGTGGAGATC ATCGCCAACG ACCAGGGCAA CCGCACGACC 
CCAAGCTACG TGGCCTTCAC CGACACCGAG CGGCTCATCG GGGACGCCGC CAAGAACCAG 
GTGGCCATGA ATCCCCAGAA CACTGTTTTT GACGCCAAAC GTCTAATTGG CAGGAAGTTT 
AATGATCCCG TCGTGCAGTC GGATATGAAG CTGTGGCCAT TTCAAGTGAT CAATGAAGCG 
GGCAAGCCTA AGGTGCTGGT GTCCTACAAA GGAGAGAAGA AAGCCTTCTA CCCGGAGGAG 
ATCTCGTCCA TGGTGCTGAC CAAGATGAAG GAGACTGCAG AGGCTTTTTT GGGCCACAGT 
GTCACCAACG CTGTGATCAC CGTGCCAGCC TATTTCAATG ACTCCCAGAG ACAGGCCACT 
AAAGATGCAG GGGTCATCGC AGGACTCAAC GTGCTGCGAA TCATCAATGA GCCCACAGCG 
GCGGCCATCG CCTACGGCTT GGATAAAGGA AGTCACGGAG AGCGGCACGT GCTCATCTTC 
GACCTGGGGG GCGGCACGTT CGACGTGTCC ATCCTGACGA TCGACGACGG CATCTTCGAG 
GTGAAGGCCA CGGCGGGCGA CACGCACCTG GGCGGGGAGG ACTTCGACAA CCGGCTGGTG 
AGCCACTTCG TGGAGGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGCCA GAACAAGCGC 
GCGGTGCGGC GTCTGCGCAC GGCGTGCGAG AGGGCCAAGA GGACGCTGTC GTCCAGCACC 
CAGGCCAACC TGGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACG 
CGGGCGCGGT TCGAGGAGCT GTGTGCAGAC CTATTTAGAG GCACGCTTGA GCCCGTGGAG 
AAGTCTCTCC GGGACGCCAA GATGGACAAG GCTAAAATCC ATGACATTGT TCTAGTAGGG 
GGCTCTACCC GCATCCCAAA GGTGCAAAAG CTGCTTCAAG ACTACTTTAA TGGACGGGAT 
CTCAACAAGA GTATCAATCC CGATGAGGCG GTGGCGTACG GAGCTGCAGT CCAGGCAGCT 
ATTTTAATGG GCGACAAATC TGAAAAAGTA CAGGATTTGC TTTTGTTGGA CGTAGCTCCC 
CTGTCTCTAG GATTGGAGAC AGCCGGGGGT GTGATGACTG TTCTGATCAA GCGCAACTCC 
ACCATCCCCA CCAAGCAGAC GCAGATCTTC ACCACCTACT CGGACAACCA GCCCGGGGTG 
CTGATCCAGG TGTACGAGGG CGAGAGGGCC ATGACGCGCG ACAACAACCT CCTGGGGCGC 
TTTGACCTGA CTGGAATACC CCCTGCACCT AGGGGTGTGC CCCAGATCGA GGTGACCTTC 
GACATCGACG CCAACGGGAT TCTCAACGTC ACAGCCATGG ACAAGAGCAC CGGCAAGGCC 
AACAAGATCA CCATCACCAA CGACAAGGGC CGCTTGAGCA AGGAGGAGAT CGAGCGCATG 
GTGCAAGAGG CCGAGCGGTA CAAGGCCGAG GACGAGGGCC AGAGGGAGAA GATCGCTGCC 
AAAAATGCCT TAGAATCGTA TGCCTTTAAC ATGAAGAGCG CTGTAGGCGA TGAGGGTCTG 
AAGGACAAGA TCAGCGAGTC TGATAAAAAG AAAATACTGG ATAAATGCAG TGAGGTCCTT 
TCCTGGCTGG AGGCCAACCA GCTGGCGGAG AAAGAAGAGT TCGATCATAA AAGAAAAGAG 
CTGGAAAACA TGTGCAACCC TATCATCACA AAGCTGTACC AGAGCGGCTG CACAGGGCCC 
ACCTGCGCGC CAGGGTATAC ACCCGGCAGG GCTGCCACAG GCCCCACCAT TGAGGAAGTA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HLA class III genes involvement in Kawasaki disease: a case-control study in Caucasian population.
International journal of immunogenetics41(1)44-53(2014 Feb)
Maggioli E,Boiocchi C,Zorzetto M,Mannarino S,Bossi G,Cuccia M

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Journal of proteomics75(12)3574-84(2012 Jun)
Bastos-Amador P,Royo F,Gonzalez E,Conde-Vancells J,Palomo-Diez L,Borras FE,Falcon-Perez JM

Heat shock protein 70 gene polymorphisms in sudden sensorineural hearing loss.
Audiology & neuro-otology17(6)381-5(2012)
Chien CY,Chang NC,Tai SY,Wang LF,Wu MT,Ho KY

The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction.
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Hspa1l cDNA ORF clone, Rattus norvegicus(Norway rat)

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