hsp70.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsp70.3
Entrez Gene ID	30671
Full Name	heat shock cognate 70-kd protein, tandem duplicate 3
Synonyms	hsp70,hsp70-4,hspa1a
General protein information	Preferred Names heat shock cognate 70-kd protein, tandem duplicate 3 Names heat shock cognate 70-kd protein etID309743.10 heat shock 70kD protein 1A stress protein HSP70
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131397.3	NP_571472.2	heat shock cognate 70-kd protein

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09602	XP_963366.1
Saccharomyces cerevisiae (baker's yeast)	SSA2	NP_013076.1
Danio rerio (zebrafish)	zgc:174006	XP_005163979.1
Eremothecium gossypii	AGOS_AFR114W	NP_985661.1
Canis lupus familiaris (dog)	HSPA1L	XP_005627220.1
Mus musculus (house mouse)	Hspa1l	NP_038586.2
Danio rerio (zebrafish)	si:ch211-199o1.2	NP_001093532.1
Danio rerio (zebrafish)	hsp70	NP_571472.1
Arabidopsis thaliana (thale cress)	Hsp70b	NP_173055.1
Magnaporthe oryzae (rice blast fungus)	MGG_06958	XP_003709704.1
Pan troglodytes (chimpanzee)	HSPA1L	XP_001160137.2
Rattus norvegicus (Norway rat)	Hspa1l	NP_997711.1
Saccharomyces cerevisiae (baker's yeast)	SSA1	NP_009396.2
Kluyveromyces lactis	KLLA0D04224g	XP_453252.1
Schizosaccharomyces pombe (fission yeast)	ssa1	NP_593704.1
Schizosaccharomyces pombe (fission yeast)	ssa2	NP_588421.1
Homo sapiens (human)	HSPA1L	NP_005518.3
Macaca mulatta (Rhesus monkey)	LOC716633	XP_002803717.1
Bos taurus (cattle)	HSPA1L	NP_001161367.1
Danio rerio (zebrafish)	hsp70l	NP_001107061.1
Danio rerio (zebrafish)	hsp70.2	XP_003198158.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay.
Ji C, Yan L, Chen Y, Yue S, Dong Q, Chen J, Zhao M
Journal of hazardous materials368514-522(2019 Apr)

Heat stress upregulates G-T mismatch binding activities in zebrafish (Danio rerio) embryos preexposed and nonexposed to a sublethal level of cadmium (Cd).
Ho TN, Paul GV, Chen YH, Hsu T
Chemosphere218179-188(2019 Mar)

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.
Jin M, Sheng W, Han L, He Q, Ji X, Liu K
Fish & shellfish immunology8326-36(2018 Dec)

Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system.
Putri RR, Chen L
Gene677273-279(2018 Nov)

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress.
Xu K, Xu H, Han Z
Genes9(12)(2018 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hsp70.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsp70.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39296	NM_131397.3 Latest version!	Danio rerio heat shock cognate 70-kd protein, tandem duplicate 3 (hsp70.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsp70.3 ( NM_131397.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571472.2 Danio rerio heat shock cognate 70-kd protein, tandem duplicate 3 (hsp70.3), mRNA.

Price & Availability↑

CloneID	ODa39296
Clone ID Related Accession (Same CDS sequence)	NM_131397.3
Accession Version	NM_131397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	heat shock cognate 70-kd protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC056709.1. On Jun 9, 2015 this sequence version replaced NM_131397.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056709.1, CA470130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCTC CAAAAGGAAT CGCTATTGGC ATTGACCTGG GCACCACCTA CTCCTGTGTG 
GGGGTGTTTC AGCATGGAAA AGTGGAGATC ATCGCCAACG ACCAGGGCAA CAGAACAACA 
CCCAGCTATG TTGCCTTCAC AGACACAGAG AGGCTGATTG GAGACGCAGC TAAGAACCAG 
GTGGCCATGA ATCCCAACAA CACGGTGTTC GATGCCAAGA GGCTGATCGG CAGGAGGTTC 
GATGACCCTG TAGTGCAGTC TGACATGAAG CACTGGTCCT TCAAAGTCGT CAGTGATGGA 
GGAAAGCCAA AAGTTGCAGT GGAACACAAA GGAGAAAACA AGACCTTTAA TCCTGAAGAG 
ATTTCCTCCA TGGTCCTGGT GAAGATGAAG GAGATTGCAG AGGCTTATCT GGGGCAGAAG 
GTGACCAACG CAGTTATCAC AGTTCCAGCC TATTTCAACG ACTCCCAGAG ACAGGCCACT 
AAAGATGCTG GAGTCATCGC TGGACTGAAC GTGCTCCGCA TCATCAATGA GCCCACGGCT 
GCAGCCATCG CCTACGGCCT GGACAAAGGC AAATCCTCAG AGCGCAACGT GCTGATCTTT 
GACCTGGGCG GAGGCACCTT CGACGTGTCT ATCCTGACCA TCGAAGACGG CATCTTTGAG 
GTGAAGGCCA CCGCTGGAGA CACTCATCTG GGCGGTGAGG ACTTTGACAA CCGCATGGTG 
AACCACTTTG TGGAAGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGTCA GAACAAGAGG 
GCCCTGAGGA GACTGCGAAC CGCATGCGAA CGAGCCAAGA GGACGCTCTC GTCCAGCTCT 
CAGGCCAGCA TTGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACC 
AGAGCTCGCT TCGAGGAGCT CTGCTCCGAC CTCTTCAGGG GAACGCTTGA TCCTGTGGAG 
AAAGCACTGA GGGACGCCAA GATGGACAAG GCTCAGATCC ACGACATCGT GCTGGTTGGG 
GGATCAACAA GAATCCCAAA GATCCAGAAG CTTCTGCAGG ATTTCTTCAA CGGCAGAGAA 
CTGAACAAGA GCATTAACCC TGATGAAGCG GTGGCTTACG GTGCCGCGGT GCAGGCCGCC 
ATCCTCATGG GCGACACCTC TGGAAACGTG CAGGACCTGC TGCTGCTGGA CGTGGCCCCA 
CTGTCTCTGG GTATTGAGAC CGCAGGTGGA GTCATGACGG CCCTCATCAA GCGCAACACA 
ACTATCCCCA CCAAACAGAC CCAGACCTTC ACCACCTACT CCGACAACCA GCCCGGCGTC 
CTGATCCAGG TGTTCGAGGG AGAAAGAGCC ATGACCAAAG ACAACAACCT GCTGGGCAAA 
TTTGAGCTGA CGGGAATTCC ACCTGCGCCA CGTGGCGTCC CGCAGATCGA AGTGACCTTC 
GACATCGACG CCAACGGGAT CCTAAATGTG TCGGCGGCGG ACAAAAGCAC CGGAAAACAG 
AACAAGATCA CCATCACCAA CGACAAGGGC AGGCTGAGCA AAGAGGAGAT CGAGAGAATG 
GTGCAGGAGG CCGACAAGTA CAAAGCTGAA GACGATCTGC AGAGAGAGAA GATTTCTGCC 
AAAAACTCCC TGGAGTCTTA CGCCTTCAAC ATGAAGAACA GTGTGGAAGA CGACAACCTG 
AAAGGCAAGA TCAGCGAGGA GGACAAGAAG AGGGTTATTG AGAAGTGCAA TGAAGCCGTG 
AGCTGGCTGG AGAACAACCA GCTGGCGGAT AAAGAGGAGT ACGAACATCA GCTGAAGGAG 
CTGGAGAAAG TCTGCAATCC AGTCATCTCC AAACTCTACC AGGGAGGGAT GCCAGCTGGA 
GGATGTGGAG CTCAGGCACG GGGCGCATCA GGGGCCAGCG CTCAGGGGCC CACCATTGAA 
GAGGTGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571472.2
CDS	125..2056
Translation	MSSPKGIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTVFDAKRLIGRRFDDPVVQSDMKHWSFKVVSDGGKPKVAVEHKGENKTFNPEEISSMVLVKMKEIAEAYLGQKVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGKSSERNVLIFDLGGGTFDVSILTIEDGIFEVKATAGDTHLGGEDFDNRMVNHFVEEFKRKHKKDISQNKRALRRLRTACERAKRTLSSSSQASIEIDSLYEGIDFYTSITRARFEELCSDLFRGTLDPVEKALRDAKMDKAQIHDIVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILMGDTSGNVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKQTQTFTTYSDNQPGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAADKSTGKQNKITITNDKGRLSKEEIERMVQEADKYKAEDDLQREKISAKNSLESYAFNMKNSVEDDNLKGKISEEDKKRVIEKCNEAVSWLENNQLADKEEYEHQLKELEKVCNPVISKLYQGGMPAGGCGAQARGASGASAQGPTIEEVD

Target ORF information:

RefSeq Version	NM_131397.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock cognate 70-kd protein, tandem duplicate 3 (hsp70.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131397.3

ORF Insert Sequence:

ATGTCCTCTC CAAAAGGAAT CGCTATTGGC ATTGACCTGG GCACCACCTA CTCCTGTGTG 
GGGGTGTTTC AGCATGGAAA AGTGGAGATC ATCGCCAACG ACCAGGGCAA CAGAACAACA 
CCCAGCTATG TTGCCTTCAC AGACACAGAG AGGCTGATTG GAGACGCAGC TAAGAACCAG 
GTGGCCATGA ATCCCAACAA CACGGTGTTC GATGCCAAGA GGCTGATCGG CAGGAGGTTC 
GATGACCCTG TAGTGCAGTC TGACATGAAG CACTGGTCCT TCAAAGTCGT CAGTGATGGA 
GGAAAGCCAA AAGTTGCAGT GGAACACAAA GGAGAAAACA AGACCTTTAA TCCTGAAGAG 
ATTTCCTCCA TGGTCCTGGT GAAGATGAAG GAGATTGCAG AGGCTTATCT GGGGCAGAAG 
GTGACCAACG CAGTTATCAC AGTTCCAGCC TATTTCAACG ACTCCCAGAG ACAGGCCACT 
AAAGATGCTG GAGTCATCGC TGGACTGAAC GTGCTCCGCA TCATCAATGA GCCCACGGCT 
GCAGCCATCG CCTACGGCCT GGACAAAGGC AAATCCTCAG AGCGCAACGT GCTGATCTTT 
GACCTGGGCG GAGGCACCTT CGACGTGTCT ATCCTGACCA TCGAAGACGG CATCTTTGAG 
GTGAAGGCCA CCGCTGGAGA CACTCATCTG GGCGGTGAGG ACTTTGACAA CCGCATGGTG 
AACCACTTTG TGGAAGAGTT CAAGAGGAAG CACAAGAAGG ACATCAGTCA GAACAAGAGG 
GCCCTGAGGA GACTGCGAAC CGCATGCGAA CGAGCCAAGA GGACGCTCTC GTCCAGCTCT 
CAGGCCAGCA TTGAGATCGA CTCTCTGTAC GAGGGCATCG ACTTCTACAC GTCCATCACC 
AGAGCTCGCT TCGAGGAGCT CTGCTCCGAC CTCTTCAGGG GAACGCTTGA TCCTGTGGAG 
AAAGCACTGA GGGACGCCAA GATGGACAAG GCTCAGATCC ACGACATCGT GCTGGTTGGG 
GGATCAACAA GAATCCCAAA GATCCAGAAG CTTCTGCAGG ATTTCTTCAA CGGCAGAGAA 
CTGAACAAGA GCATTAACCC TGATGAAGCG GTGGCTTACG GTGCCGCGGT GCAGGCCGCC 
ATCCTCATGG GCGACACCTC TGGAAACGTG CAGGACCTGC TGCTGCTGGA CGTGGCCCCA 
CTGTCTCTGG GTATTGAGAC CGCAGGTGGA GTCATGACGG CCCTCATCAA GCGCAACACA 
ACTATCCCCA CCAAACAGAC CCAGACCTTC ACCACCTACT CCGACAACCA GCCCGGCGTC 
CTGATCCAGG TGTTCGAGGG AGAAAGAGCC ATGACCAAAG ACAACAACCT GCTGGGCAAA 
TTTGAGCTGA CGGGAATTCC ACCTGCGCCA CGTGGCGTCC CGCAGATCGA AGTGACCTTC 
GACATCGACG CCAACGGGAT CCTAAATGTG TCGGCGGCGG ACAAAAGCAC CGGAAAACAG 
AACAAGATCA CCATCACCAA CGACAAGGGC AGGCTGAGCA AAGAGGAGAT CGAGAGAATG 
GTGCAGGAGG CCGACAAGTA CAAAGCTGAA GACGATCTGC AGAGAGAGAA GATTTCTGCC 
AAAAACTCCC TGGAGTCTTA CGCCTTCAAC ATGAAGAACA GTGTGGAAGA CGACAACCTG 
AAAGGCAAGA TCAGCGAGGA GGACAAGAAG AGGGTTATTG AGAAGTGCAA TGAAGCCGTG 
AGCTGGCTGG AGAACAACCA GCTGGCGGAT AAAGAGGAGT ACGAACATCA GCTGAAGGAG 
CTGGAGAAAG TCTGCAATCC AGTCATCTCC AAACTCTACC AGGGAGGGAT GCCAGCTGGA 
GGATGTGGAG CTCAGGCACG GGGCGCATCA GGGGCCAGCG CTCAGGGGCC CACCATTGAA 
GAGGTGGATT AA