Prkca cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkca
Entrez Gene ID	24680
Full Name	protein kinase C, alpha
Synonyms	Pkca
General protein information	Preferred Names protein kinase C, alpha Names protein kinase C alpha type PKC-A PKC-alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	displays Ca+2 dependent protein kinase activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597007.1	XP_017452496.1	protein kinase C alpha type isoform X2
XM_017597006.1	XP_017452495.1	protein kinase C alpha type isoform X1
NM_001105713.1	NP_001099183.1	protein kinase C alpha type

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prkca	NP_001099183.1
Drosophila melanogaster (fruit fly)	Pkc53E	NP_725626.1
Mus musculus (house mouse)	Prkca	NP_035231.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012252	XP_320290.4
Homo sapiens (human)	PRKCA	NP_002728.1
Canis lupus familiaris (dog)	PRKCA	XP_005624328.1
Bos taurus (cattle)	PRKCA	NP_776860.1
Gallus gallus (chicken)	PRKCA	NP_001012822.1
Danio rerio (zebrafish)	prkca	NP_001243170.1
Macaca mulatta (Rhesus monkey)	PRKCA	XP_002800615.1
Caenorhabditis elegans (roundworm)	pkc-2	NP_001024518.1
Gallus gallus (chicken)	LOC101749801	XP_004946286.1
Pan troglodytes (chimpanzee)	PRKCA	XP_003315734.1
Xenopus tropicalis (tropical clawed frog)	prkca	NP_001072752.1

Gene Ontology
Bibliography

Related articles in PubMed

PKCα-Mediated Downregulation of RhoA Activity in Depolarized Vascular Smooth Muscle: Synergistic Vasorelaxant Effect of PKCα and ROCK Inhibition.
González-Montelongo MDC, Porras-González C, González-Montelongo R, Revilla-González G, Pastor MD, Castellano A, Ureña J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(1)76-93(2019)

PKCα replaces AMPK to regulate mitophagy: Another PEDF role on ischaemic cardioprotection.
Miao H, Qiu F, Huang B, Liu X, Zhang H, Liu Z, Yuan Y, Zhao Q, Zhang H, Dong H, Zhang Z
Journal of cellular and molecular medicine22(11)5732-5742(2018 11)

TLR4/PKCα/occludin signaling pathway may be related to blood‑brain barrier damage.
Tang Z, Guo D, Xiong L, Wu B, Xu X, Fu J, Kong L, Liu Z, Xie C
Molecular medicine reports18(1)1051-1057(2018 Jul)

PKC-Mediated Modulation of Astrocyte SNAT3 Glutamine Transporter Function at Synapses in Situ.
Dong W, Todd AC, Bröer A, Hulme SR, Bröer S, Billups B
International journal of molecular sciences19(4)(2018 Mar)

Identification of the major phosphorylation sites for protein kinase C in kit/stem cell factor receptor in vitro and in intact cells.
Blume-Jensen P, Wernstedt C, Heldin CH, Rönnstrand L
The Journal of biological chemistry270(23)14192-200(1995 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prkca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12695	NM_001105713.1 Latest version!	Rattus norvegicus protein kinase C, alpha (Prkca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa57176	XM_017597006.1 Latest version!	Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa57177	XM_017597007.1 Latest version!	Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkca ( NM_001105713.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099183.1 Rattus norvegicus protein kinase C, alpha (Prkca), mRNA.

Price & Availability↑

CloneID	ORa12695
Clone ID Related Accession (Same CDS sequence)	NM_001105713.1
Accession Version	NM_001105713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C alpha type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_343975.3, XM_001081588.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGGA CTGTTCTTTC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCTTAT GACCAAACAC 
CCTGCCAAGC GGCTGGGCTG CGGGCCCGAG GGGGAAAGGG ATGTCAGAGA GCATGCCTTC 
TTTAGGAGGA TCGACTGGGA GAAGTTGGAG AACAGGGAGA TCCAACCGCC ATTCAAGCCC 
AAAGTGTGCG GCAAAGGAGC AGAAAACTTT GACAAGTTCT TCACACGAGG GCAGCCTGTC 
TTAACACCAC CAGATCAGCT GGTCATCGCT AACATAGACC AGTCTGATTT TGAAGGGTTC 
TCGTATGTCA ACCCCCAGTT TGTGCACCCA ATCTTGCAAA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099183.1
CDS	269..2179
Translation	MADVYPANDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	NM_001105713.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, alpha (Prkca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105713.1

ORF Insert Sequence:

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGGA CTGTTCTTTC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCTTAT GACCAAACAC 
CCTGCCAAGC GGCTGGGCTG CGGGCCCGAG GGGGAAAGGG ATGTCAGAGA GCATGCCTTC 
TTTAGGAGGA TCGACTGGGA GAAGTTGGAG AACAGGGAGA TCCAACCGCC ATTCAAGCCC 
AAAGTGTGCG GCAAAGGAGC AGAAAACTTT GACAAGTTCT TCACACGAGG GCAGCCTGTC 
TTAACACCAC CAGATCAGCT GGTCATCGCT AACATAGACC AGTCTGATTT TGAAGGGTTC 
TCGTATGTCA ACCCCCAGTT TGTGCACCCA ATCTTGCAAA GTGCAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkca ( XM_017597006.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452495.1 Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57176
Clone ID Related Accession (Same CDS sequence)	XM_017597006.1
Accession Version	XM_017597006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C alpha type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGAC TGTTCGTTCC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCCTCC GTTCGATGGC 
GAAGACGAAG ATGAACTGTT TCAGTCTATA ATGGAGCACA ATGTGTCCTA CCCCAAATCC 
TTGTCCAAGG AAGCTGTCTC CATCTGCAAA GGGCTTATGA CCAAACACCC TGCCAAGCGG 
CTGGGCTGCG GGCCCGAGGG GGAAAGGGAT GTCAGAGAGC ATGCCTTCTT TAGGAGGATC 
GACTGGGAGA AGTTGGAGAA CAGGGAGATC CAACCGCCAT TCAAGCCCAA AGTGAATTTC 
TGTACCAAAA TGCACTGGCT TCAGTGGGCA TCCAGGCCTT CGTGCGGCAA AGGAGCAGAA 
AACTTTGACA AGTTCTTCAC ACGAGGGCAG CCTGTCTTAA CACCACCAGA TCAGCTGGTC 
ATCGCTAACA TAGACCAGTC TGATTTTGAA GGGTTCTCGT ATGTCAACCC CCAGTTTGTG 
CACCCAATCT TGCAAAGTGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452495.1
CDS	490..2556
Translation	MADVYPANDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIDCSFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVNFCTKMHWLQWASRPSCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	XM_017597006.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597006.1

ORF Insert Sequence:

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGAC TGTTCGTTCC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCCTCC GTTCGATGGC 
GAAGACGAAG ATGAACTGTT TCAGTCTATA ATGGAGCACA ATGTGTCCTA CCCCAAATCC 
TTGTCCAAGG AAGCTGTCTC CATCTGCAAA GGGCTTATGA CCAAACACCC TGCCAAGCGG 
CTGGGCTGCG GGCCCGAGGG GGAAAGGGAT GTCAGAGAGC ATGCCTTCTT TAGGAGGATC 
GACTGGGAGA AGTTGGAGAA CAGGGAGATC CAACCGCCAT TCAAGCCCAA AGTGAATTTC 
TGTACCAAAA TGCACTGGCT TCAGTGGGCA TCCAGGCCTT CGTGCGGCAA AGGAGCAGAA 
AACTTTGACA AGTTCTTCAC ACGAGGGCAG CCTGTCTTAA CACCACCAGA TCAGCTGGTC 
ATCGCTAACA TAGACCAGTC TGATTTTGAA GGGTTCTCGT ATGTCAACCC CCAGTTTGTG 
CACCCAATCT TGCAAAGTGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkca ( XM_017597007.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452496.1 Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57177
Clone ID Related Accession (Same CDS sequence)	XM_017597007.1
Accession Version	XM_017597007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C alpha type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGAC TGTTCGTTCC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCCTCC GTTCGATGGC 
GAAGACGAAG ATGAACTGTT TCAGTCTATA ATGGAGCACA ATGTGTCCTA CCCCAAATCC 
TTGTCCAAGG AAGCTGTCTC CATCTGCAAA GGGCTTATGA CCAAACACCC TGCCAAGCGG 
CTGGGCTGCG GGCCCGAGGG GGAAAGGGAT GTCAGAGAGC ATGCCTTCTT TAGGAGGATC 
GACTGGGAGA AGTTGGAGAA CAGGGAGATC CAACCGCCAT TCAAGCCCAA AGTGTGCGGC 
AAAGGAGCAG AAAACTTTGA CAAGTTCTTC ACACGAGGGC AGCCTGTCTT AACACCACCA 
GATCAGCTGG TCATCGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCAAT CTTGCAAAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452496.1
CDS	482..2500
Translation	MADVYPANDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIDCSFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	XM_017597007.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, alpha (Prkca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597007.1

ORF Insert Sequence:

ATGGCTGACG TTTACCCGGC CAACGACTCC ACGGCGTCTC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCATGAGG TGAAAGACCA CAAATTCATC 
GCCCGCTTCT TCAAGCAACC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGAAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAG 
TTTGTTACTT TCTCTTGTCC GGGTGCGGAT AAGGGACCTG ACACTGATGA CCCCAGAAGC 
AAGCACAAGT TCAAAATCCA CACCTATGGA AGCCCTACCT TCTGTGATCA CTGTGGGTCC 
CTGCTCTACG GACTTATCCA CCAAGGGATG AAATGCGACA CCTGCGACAT GAATGTTCAC 
AAGCAGTGCG TGATCAATGT CCCCAGCCTC TGCGGAATGG ATCACACAGA GAAGAGGGGG 
CGGATTTACC TGAAGGCAGA GGTCACAGAT GAAAAGCTGC ACGTCACCGT ACGAGATGCA 
AAAAATCTAA TCCCTATGGA TCCAAATGGG CTTTCGGATC CTTACGTGAA GCTGAAACTT 
ATTCCTGACC CCAAGAATGA GAGCAAACAG AAAACCAAAA CCATCCGATC CACACTGAAC 
CCTCAGTGGA ATGAGTCCTT CACGTTCAAA TTAAAACCTT CAGACAAAGA CCGGCGACTG 
TCCGTAGAAA TCTGGGACTG GGATCGGACG ACACGGAATG ACTTCATGGG CTCCCTTTCC 
TTCGGCGTCT CAGAGCTGAT GAAGATGCCA GCCAGTGGAT GGTACAAGTT GCTCAACCAA 
GAGGAGGGTG AATACTACAA TGTGCCCATT CCAGAAGGAG ATGAAGAAGG CAACGTGGAA 
CTCAGGCAGA AGTTCGAGAA AGCCAAGCTG GGCCCCGCTG GAAACAAAGT CATCAGCCCT 
TCAGAAGACA GGAAGCAGCC ATCTAACAAC CTGGACAGGG TGAAACTCAC AGACTTCAAC 
TTCCTCATGG TGCTGGGGAA GGGGAGTTTT GGAAAGGTGA TGCTTGCTGA CAGGAAGGGA 
ACAGAGGAAC TGTACGCCAT CAAAATCCTG AAGAAGGACG TGGTGATCCA GGATGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTT CTGGCCCTGC TCGACAAGCC CCCGTTCCTG 
ACACAGCTGC ACTCCTGCTT CCAGACAGTG GACCGGCTGT ACTTCGTCAT GGAATACGTC 
AACGGTGGGG ACCTCATGTA CCACATTCAG CAAGTCGGAA AATTTAAGGA GCCACAAGCA 
GTATTCTATG CAGCCGAGAT CTCCATCGAC TGTTCGTTCC TTCACAAAAG AGGAATCATT 
TACAGGGATC TGAAGCTGGA CAACGTCATG CTGGACTCAG AAGGGCATAT CAAAATCGCC 
GACTTCGGGA TGTGCAAGGA ACACATGATG GACGGGGTCA CGACCAGGAC CTTCTGTGGG 
ACTCCGGATT ACATTGCCCC AGAGATAATC GCTTACCAGC CATATGGAAA GTCTGTGGAC 
TGGTGGGCGT ACGGCGTGCT CCTGTATGAG ATGCTAGCTG GGCAGCCTCC GTTCGATGGC 
GAAGACGAAG ATGAACTGTT TCAGTCTATA ATGGAGCACA ATGTGTCCTA CCCCAAATCC 
TTGTCCAAGG AAGCTGTCTC CATCTGCAAA GGGCTTATGA CCAAACACCC TGCCAAGCGG 
CTGGGCTGCG GGCCCGAGGG GGAAAGGGAT GTCAGAGAGC ATGCCTTCTT TAGGAGGATC 
GACTGGGAGA AGTTGGAGAA CAGGGAGATC CAACCGCCAT TCAAGCCCAA AGTGTGCGGC 
AAAGGAGCAG AAAACTTTGA CAAGTTCTTC ACACGAGGGC AGCCTGTCTT AACACCACCA 
GATCAGCTGG TCATCGCTAA CATAGACCAG TCTGATTTTG AAGGGTTCTC GTATGTCAAC 
CCCCAGTTTG TGCACCCAAT CTTGCAAAGT GCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PKCα-Mediated Downregulation of RhoA Activity in Depolarized Vascular Smooth Muscle: Synergistic Vasorelaxant Effect of PKCα and ROCK Inhibition.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(1)76-93(2019)
González-Montelongo MDC,Porras-González C,González-Montelongo R,Revilla-González G,Pastor MD,Castellano A,Ureña J

PKCα replaces AMPK to regulate mitophagy: Another PEDF role on ischaemic cardioprotection.
Journal of cellular and molecular medicine22(11)5732-5742(2018 11)
Miao H,Qiu F,Huang B,Liu X,Zhang H,Liu Z,Yuan Y,Zhao Q,Zhang H,Dong H,Zhang Z

TLR4/PKCα/occludin signaling pathway may be related to blood‑brain barrier damage.
Molecular medicine reports18(1)1051-1057(2018 Jul)
Tang Z,Guo D,Xiong L,Wu B,Xu X,Fu J,Kong L,Liu Z,Xie C

PKC-Mediated Modulation of Astrocyte SNAT3 Glutamine Transporter Function at Synapses in Situ.
International journal of molecular sciences19(4)(2018 Mar)
Dong W,Todd AC,Bröer A,Hulme SR,Bröer S,Billups B

Identification of the major phosphorylation sites for protein kinase C in kit/stem cell factor receptor in vitro and in intact cells.
The Journal of biological chemistry270(23)14192-200(1995 Jun)
Blume-Jensen P,Wernstedt C,Heldin CH,Rönnstrand L

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Prkca cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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