prkca cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkca
Entrez Gene ID	497384
Full Name	protein kinase C, alpha
Synonyms	im:7139045,si:dkey-8l19.1
General protein information	Preferred Names protein kinase C, alpha Names protein kinase C alpha type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256241.1	NP_001243170.1	protein kinase C alpha type
XM_021476862.1	XP_021332537.1	protein kinase C alpha type isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prkca	NP_001099183.1
Drosophila melanogaster (fruit fly)	Pkc53E	NP_725626.1
Mus musculus (house mouse)	Prkca	NP_035231.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012252	XP_320290.4
Homo sapiens (human)	PRKCA	NP_002728.1
Canis lupus familiaris (dog)	PRKCA	XP_005624328.1
Bos taurus (cattle)	PRKCA	NP_776860.1
Gallus gallus (chicken)	PRKCA	NP_001012822.1
Danio rerio (zebrafish)	prkca	NP_001243170.1
Macaca mulatta (Rhesus monkey)	PRKCA	XP_002800615.1
Caenorhabditis elegans (roundworm)	pkc-2	NP_001024518.1
Gallus gallus (chicken)	LOC101749801	XP_004946286.1
Pan troglodytes (chimpanzee)	PRKCA	XP_003315734.1
Xenopus tropicalis (tropical clawed frog)	prkca	NP_001072752.1

Gene Ontology
Bibliography

Related articles in PubMed

C9orf72 is essential for neurodevelopment and motility mediated by Cyclin G1.
Yeh TH, Liu HF, Li YW, Lu CS, Shih HY, Chiu CC, Lin SJ, Huang YC, Cheng YC
Experimental neurology304114-124(2018 06)

Phylogeny and distribution of protein kinase C variants in the zebrafish.
Haug MF, Gesemann M, Berger M, Neuhauss SCF
The Journal of comparative neurology526(7)1097-1109(2018 05)

Genetic Reduction in Left Ventricular Protein Kinase C-α and Adverse Ventricular Remodeling in Human Subjects.
Hu R, Morley MP, Brandimarto J, Tucker NR, Parsons VA, Zhao SD, Meder B, Katus HA, Rühle F, Stoll M, Villard E, Cambien F, Lin H, Smith NL, Felix JF, Vasan RS, van der Harst P, Newton-Cheh C, Li J, Kim CE, Hakonarson H, Hannenhalli S, Ashley EA, Moravec CS, Tang WHW, Maillet M, Molkentin JD, Ellinor PT, Margulies KB, Cappola TP
Circulation. Genomic and precision medicine11(3)e001901(2018 03)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio).
Gao J, Mahapatra CT, Mapes CD, Khlebnikova M, Wei A, Sepúlveda MS
Nanotoxicology10(9)1363-72(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prkca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35844	NM_001256241.1 Latest version!	Danio rerio protein kinase C, alpha, a (prkcaa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa142962	XM_021476862.1 Latest version!	Danio rerio protein kinase C, alpha (prkca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkca ( NM_001256241.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001243170.1 Danio rerio protein kinase C, alpha, a (prkcaa), mRNA.

Price & Availability↑

CloneID	ODa35844
Clone ID Related Accession (Same CDS sequence)	NM_001256241.1
Accession Version	NM_001256241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	protein kinase C alpha type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX936378.12 and CR759902.6. On Jan 12, 2012 this sequence version replaced XM_002662404.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGATA CACAAAGCAA CGAGCAATCC TCGTCTGACG TTGCAAATCG ATTTGCCCGT 
AAAGGTGCTT TGAGGCAGAA AAACGTCCAT GAAGTGAAAA ACCATAAATT CACCGCGAGG 
TTTTTCAAGC AGCCGACTTT CTGCAGTCAT TGCACTGACT TCATTTGGGG GTTTGGAAAG 
CAAGGATTTC AGTGCCAAGT GTGCTGTTTT GTTGTCCATA AACGTTGCCA TGAACTTGTC 
ACATTCTCAT GCCCTGGAGC AGATAAAGGG CCAGATACAG ATGATCCTAG GAGCAAGCAC 
AAATTTAAGA TCCACACTTA TGGCAGTCCC ACATTCTGTG ACCACTGTGG GTCTCTTCTA 
TATGGACTCA TACACCAAGG AATGAAATGT GACTCCTGTG ATATGAATGT CCATAAGCAG 
TGTGTGATGA ATGTGCCAAG CCTTTGTGGA ACGGACCACA CAGAGAGACG GGGACGCCTT 
TACATGAAGA TTGAGGTGAT TGGCGACAAA ATGCATGTCA CAGTTGGTGA AGGCAAGAAC 
TTGATTCCGA TGGACCCAAA TGGATTGTCT GATCCATATG TAAAACTCAA GTTGATTCCA 
GACCCTAAAA ATGAGACTAA ACAGAAAACA AAAACCATTC GCTCCTCCCT GAATCCAACT 
TGGAACGAAA CGTTTACATT TAAGCTGAAG CCAGCAGATA AGGACCGCCG ACTGGCTGTG 
GAGGTATGGG ACTGGGACAG AACCACCCGC AATGACTTCA TGGGCTCAAT GTCTTTTGGT 
GTGTCGGAGC TCATGAAGTC CCCAGTATGT GGCTGGTACA AAATGTTAAA CCAGGAGGAG 
GGGGAGTACT ATAATGTGCC CATTCCAGAT GAAGATGACC CCGATACTGA ACTCCGACAG 
AAATTTGAGA TAGCCAAATT GGGTCCTCCC AAAAAAAACA TTACCGCTTT GGATGACAGT 
GGGTCCAGCA TAACTTCCAG CAATGTGGAC AATGTCAGGC TCTCAGATTT TAATTTCCTG 
GCAGTACTTG GAAAAGGCAG CTTTGGAAAG GTCATGCTGG CAGAGATGAA GTCTTCTGAT 
GAACTATTTG CTATAAAAAT CCTGAAGAAG GATGTGGTCA TCCAGGATGA CGATGTTGAA 
TGCACCATGG TGGAGAAGCG AGTTCTGGCT CAGCAGGACA AGCCTCCATT CCTCACCCAG 
CTTCATTCCT GCTTCCAGAC TGTGGATCGC CTGTATTTTG TGATGGAGTA TGTTAATGGT 
GGTGATCTTA TGTATCACAT CCAGCAAGTA GGCAAATTTA AGGAGCCCCA GGCTGTGTTC 
TATGCTGCAG AGATTGCTGT TGGGCTCTTC TTCTTGCACA AGAAAGGAAT TATATACAGA 
GACCTAAAAT TAGATAATGT TATGCTGGAC GCAGAGGGTC ATATTAAGAT TGCTGACTTT 
GGTATGTGCA AGGAGAACAT TGTGGAGGGT GTGACCACCC GTACATTTTG TGGAACGCCA 
GACTACATCG CTCCAGAGAT AATAGCATAT CAGCCATATG GTCGCTCTGT GGACTGGTGG 
GCTTATGGAG TCCTTCTGTA TGAGATGCTG GCTGGACAAC CTCCATTTGA TGGTGAGGAT 
GAAGATGAAC TTTTCCAGTC TATTATGGAA CACAATGTCT CCTACTCTAA AACCTTGTCC 
AAAGAAGCAG TTTCCATCTG CAAAGGGCTG ATGACCAAAC ACCCTTCAAA ACGCCTGGGC 
TGTGGTCAAG AGGGTGAGAG AGATATTAGG GAACATGCTT TCTTCCGTCG CATTGACTGG 
GATCGCCTGG CAAACAGAGA AGTACAGCCT CCATTCAAAC CCAAAGTGTG TGGAAAGGGG 
GCAGAAAACT TTGACAAGTT CTTCACTCGC ACGCAGCCAC AACTGACCCC TCCTGACGAG 
CTGGTTATTG CCAACATCGA CCAGGCTGAT TTCGATGGAT TTACCTTCGT CAATCCTCAA 
TTTGTCCATC CCTCCCTTGT CAACACTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243170.1
CDS	160..2172
Translation	MADTQSNEQSSSDVANRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHELVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDSCDMNVHKQCVMNVPSLCGTDHTERRGRLYMKIEVIGDKMHVTVGEGKNLIPMDPNGLSDPYVKLKLIPDPKNETKQKTKTIRSSLNPTWNETFTFKLKPADKDRRLAVEVWDWDRTTRNDFMGSMSFGVSELMKSPVCGWYKMLNQEEGEYYNVPIPDEDDPDTELRQKFEIAKLGPPKKNITALDDSGSSITSSNVDNVRLSDFNFLAVLGKGSFGKVMLAEMKSSDELFAIKILKKDVVIQDDDVECTMVEKRVLAQQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEIAVGLFFLHKKGIIYRDLKLDNVMLDAEGHIKIADFGMCKENIVEGVTTRTFCGTPDYIAPEIIAYQPYGRSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYSKTLSKEAVSICKGLMTKHPSKRLGCGQEGERDIREHAFFRRIDWDRLANREVQPPFKPKVCGKGAENFDKFFTRTQPQLTPPDELVIANIDQADFDGFTFVNPQFVHPSLVNTE

Target ORF information:

RefSeq Version	NM_001256241.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, alpha, a (prkcaa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256241.1

ORF Insert Sequence:

ATGGCTGATA CACAAAGCAA CGAGCAATCC TCGTCTGACG TTGCAAATCG ATTTGCCCGT 
AAAGGTGCTT TGAGGCAGAA AAACGTCCAT GAAGTGAAAA ACCATAAATT CACCGCGAGG 
TTTTTCAAGC AGCCGACTTT CTGCAGTCAT TGCACTGACT TCATTTGGGG GTTTGGAAAG 
CAAGGATTTC AGTGCCAAGT GTGCTGTTTT GTTGTCCATA AACGTTGCCA TGAACTTGTC 
ACATTCTCAT GCCCTGGAGC AGATAAAGGG CCAGATACAG ATGATCCTAG GAGCAAGCAC 
AAATTTAAGA TCCACACTTA TGGCAGTCCC ACATTCTGTG ACCACTGTGG GTCTCTTCTA 
TATGGACTCA TACACCAAGG AATGAAATGT GACTCCTGTG ATATGAATGT CCATAAGCAG 
TGTGTGATGA ATGTGCCAAG CCTTTGTGGA ACGGACCACA CAGAGAGACG GGGACGCCTT 
TACATGAAGA TTGAGGTGAT TGGCGACAAA ATGCATGTCA CAGTTGGTGA AGGCAAGAAC 
TTGATTCCGA TGGACCCAAA TGGATTGTCT GATCCATATG TAAAACTCAA GTTGATTCCA 
GACCCTAAAA ATGAGACTAA ACAGAAAACA AAAACCATTC GCTCCTCCCT GAATCCAACT 
TGGAACGAAA CGTTTACATT TAAGCTGAAG CCAGCAGATA AGGACCGCCG ACTGGCTGTG 
GAGGTATGGG ACTGGGACAG AACCACCCGC AATGACTTCA TGGGCTCAAT GTCTTTTGGT 
GTGTCGGAGC TCATGAAGTC CCCAGTATGT GGCTGGTACA AAATGTTAAA CCAGGAGGAG 
GGGGAGTACT ATAATGTGCC CATTCCAGAT GAAGATGACC CCGATACTGA ACTCCGACAG 
AAATTTGAGA TAGCCAAATT GGGTCCTCCC AAAAAAAACA TTACCGCTTT GGATGACAGT 
GGGTCCAGCA TAACTTCCAG CAATGTGGAC AATGTCAGGC TCTCAGATTT TAATTTCCTG 
GCAGTACTTG GAAAAGGCAG CTTTGGAAAG GTCATGCTGG CAGAGATGAA GTCTTCTGAT 
GAACTATTTG CTATAAAAAT CCTGAAGAAG GATGTGGTCA TCCAGGATGA CGATGTTGAA 
TGCACCATGG TGGAGAAGCG AGTTCTGGCT CAGCAGGACA AGCCTCCATT CCTCACCCAG 
CTTCATTCCT GCTTCCAGAC TGTGGATCGC CTGTATTTTG TGATGGAGTA TGTTAATGGT 
GGTGATCTTA TGTATCACAT CCAGCAAGTA GGCAAATTTA AGGAGCCCCA GGCTGTGTTC 
TATGCTGCAG AGATTGCTGT TGGGCTCTTC TTCTTGCACA AGAAAGGAAT TATATACAGA 
GACCTAAAAT TAGATAATGT TATGCTGGAC GCAGAGGGTC ATATTAAGAT TGCTGACTTT 
GGTATGTGCA AGGAGAACAT TGTGGAGGGT GTGACCACCC GTACATTTTG TGGAACGCCA 
GACTACATCG CTCCAGAGAT AATAGCATAT CAGCCATATG GTCGCTCTGT GGACTGGTGG 
GCTTATGGAG TCCTTCTGTA TGAGATGCTG GCTGGACAAC CTCCATTTGA TGGTGAGGAT 
GAAGATGAAC TTTTCCAGTC TATTATGGAA CACAATGTCT CCTACTCTAA AACCTTGTCC 
AAAGAAGCAG TTTCCATCTG CAAAGGGCTG ATGACCAAAC ACCCTTCAAA ACGCCTGGGC 
TGTGGTCAAG AGGGTGAGAG AGATATTAGG GAACATGCTT TCTTCCGTCG CATTGACTGG 
GATCGCCTGG CAAACAGAGA AGTACAGCCT CCATTCAAAC CCAAAGTGTG TGGAAAGGGG 
GCAGAAAACT TTGACAAGTT CTTCACTCGC ACGCAGCCAC AACTGACCCC TCCTGACGAG 
CTGGTTATTG CCAACATCGA CCAGGCTGAT TTCGATGGAT TTACCTTCGT CAATCCTCAA 
TTTGTCCATC CCTCCCTTGT CAACACTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkca ( XM_021476862.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332537.1 Danio rerio protein kinase C, alpha (prkca), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142962
Clone ID Related Accession (Same CDS sequence)	XM_021476862.1
Accession Version	XM_021476862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein kinase C alpha type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA CACAAAGCAA CGAGCAATCC TCGTCTGACG TTGCAAATCG ATTTGCCCGT 
AAAGGTGCTT TGAGGCAGAA AAACGTCCAT GAAGTGAAAA ACCATAAATT CACCGCGAGG 
TTTTTCAAGC AGCCGACTTT CTGCAGTCAT TGCACTGACT TCATTTGGGG GTTTGGAAAG 
CAAGGATTTC AGTGCCAAGT GTGCTGTTTT GTTGTCCATA AACGTTGCCA TGAACTTGTC 
ACATTCTCAT GCCCTGGAGC AGATAAAGGG CCAGATACAG ATGATCCTAG GAGCAAGCAC 
AAATTTAAGA TCCACACTTA TGGCAGTCCC ACATTCTGTG ACCACTGTGG GTCTCTTCTA 
TATGGACTCA TACACCAAGG AATGAAATGT GACTCCTGTG ATATGAATGT CCATAAGCAG 
TGTGTGATGA ATGTGCCAAG CCTTTGTGGA ACGGACCACA CAGAGAGACG GGGACGCCTT 
TACATGAAGA TTGAGGTGAT TGGCGACAAA ATGCATGTCA CAGTTGGTGA AGGCAAGAAC 
TTGATTCCGA TGGACCCAAA TGGATTGTCT GATCCATATG TAAAACTCAA GTTGATTCCA 
GACCCTAAAA ATGAGACTAA ACAGAAAACA AAAACCATTC GCTCCTCCCT GAATCCAACT 
TGGAACGAAA CGTTTACATT TAAGCTGAAG CCAGCAGATA AGGACCGCCG ACTGGCTGTG 
GAGGTATGGG ACTGGGACAG AACCACCCGC AATGACTTCA TGGGCTCAAT GTCTTTTGGT 
GTGTCGGAGC TCATGAAGTC CCCAGTATGT GGCTGGTACA AAATGTTAAA CCAGGAGGAG 
GGGGAGTACT ATAATGTGCC CATTCCAGAT GAAGATGACC CCGATACTGA ACTCCGACAG 
AAATTTGAGG ATGAGACATT AGAGGACCTT GAGCAGATAG CCAAATTGGG TCCTCCCAAA 
AAAAACATTA CCGCTTTGGA TGACAGTGGG TCCAGCATAA CTTCCAGCAA TGTGGACAAT 
GTCAGGCTCT CAGATTTTAA TTTCCTGGCA GTACTTGGAA AAGGCAGCTT TGGAAAGGTC 
ATGCTGGCAG AGATGAAGTC TTCTGATGAA CTATTTGCTA TAAAAATCCT GAAGAAGGAT 
GTGGTCATCC AGGATGACGA TGTTGAATGC ACCATGGTGG AGAAGCGAGT TCTGGCTCAG 
CAGGACAAGC CTCCATTCCT CACCCAGCTT CATTCCTGCT TCCAGACTGT GGATCGCCTG 
TATTTTGTGA TGGAGTATGT TAATGGTGGT GATCTTATGT ATCACATCCA GCAAGTAGGC 
AAATTTAAGG AGCCCCAGGC TGTGTTCTAT GCTGCAGAGA TTGCTGTTGG GCTCTTCTTC 
TTGCACAAGA AAGGAATTAT ATACAGAGAC CTAAAATTAG ATAATGTTAT GCTGGACGCA 
GAGGGTCATA TTAAGATTGC TGACTTTGGT ATGTGCAAGG AGAACATTGT GGAGGGTGTG 
ACCACCCGTA CATTTTGTGG AACGCCAGAC TACATCGCTC CAGAGATAAT AGCATATCAG 
CCATATGGTC GCTCTGTGGA CTGGTGGGCT TATGGAGTCC TTCTGTATGA GATGCTGGCT 
GGACAACCTC CATTTGATGG TGAGGATGAA GATGAACTTT TCCAGTCTAT TATGGAACAC 
AATGTCTCCT ACTCTAAAAC CTTGTCCAAA GAAGCAGTTT CCATCTGCAA AGGGCTGATG 
ACCAAACACC CTTCAAAACG CCTGGGCTGT GGTCAAGAGG GTGAGAGAGA TATTAGGGAA 
CATGCTTTCT TCCGTCGCAT TGACTGGGAT CGCCTGGCAA ACAGAGAAGT ACAGCCTCCA 
TTCAAACCCA AAGTGTGTGG AAAGGGGGCA GAAAACTTTG ACAAGTTCTT CACTCGCACG 
CAGCCACAAC TGACCCCTCC TGACGAGCTG GTTATTGCCA ACATCGACCA GGCTGATTTC 
GATGGATTTA CCTTCGTCAA TCCTCAATTT GTCCATCCCT CCCTTGTCAA CACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332537.1
CDS	210..2249
Translation	MADTQSNEQSSSDVANRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHELVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDSCDMNVHKQCVMNVPSLCGTDHTERRGRLYMKIEVIGDKMHVTVGEGKNLIPMDPNGLSDPYVKLKLIPDPKNETKQKTKTIRSSLNPTWNETFTFKLKPADKDRRLAVEVWDWDRTTRNDFMGSMSFGVSELMKSPVCGWYKMLNQEEGEYYNVPIPDEDDPDTELRQKFEDETLEDLEQIAKLGPPKKNITALDDSGSSITSSNVDNVRLSDFNFLAVLGKGSFGKVMLAEMKSSDELFAIKILKKDVVIQDDDVECTMVEKRVLAQQDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEIAVGLFFLHKKGIIYRDLKLDNVMLDAEGHIKIADFGMCKENIVEGVTTRTFCGTPDYIAPEIIAYQPYGRSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYSKTLSKEAVSICKGLMTKHPSKRLGCGQEGERDIREHAFFRRIDWDRLANREVQPPFKPKVCGKGAENFDKFFTRTQPQLTPPDELVIANIDQADFDGFTFVNPQFVHPSLVNTE

Target ORF information:

RefSeq Version	XM_021476862.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, alpha (prkca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476862.1

ORF Insert Sequence:

ATGGCTGATA CACAAAGCAA CGAGCAATCC TCGTCTGACG TTGCAAATCG ATTTGCCCGT 
AAAGGTGCTT TGAGGCAGAA AAACGTCCAT GAAGTGAAAA ACCATAAATT CACCGCGAGG 
TTTTTCAAGC AGCCGACTTT CTGCAGTCAT TGCACTGACT TCATTTGGGG GTTTGGAAAG 
CAAGGATTTC AGTGCCAAGT GTGCTGTTTT GTTGTCCATA AACGTTGCCA TGAACTTGTC 
ACATTCTCAT GCCCTGGAGC AGATAAAGGG CCAGATACAG ATGATCCTAG GAGCAAGCAC 
AAATTTAAGA TCCACACTTA TGGCAGTCCC ACATTCTGTG ACCACTGTGG GTCTCTTCTA 
TATGGACTCA TACACCAAGG AATGAAATGT GACTCCTGTG ATATGAATGT CCATAAGCAG 
TGTGTGATGA ATGTGCCAAG CCTTTGTGGA ACGGACCACA CAGAGAGACG GGGACGCCTT 
TACATGAAGA TTGAGGTGAT TGGCGACAAA ATGCATGTCA CAGTTGGTGA AGGCAAGAAC 
TTGATTCCGA TGGACCCAAA TGGATTGTCT GATCCATATG TAAAACTCAA GTTGATTCCA 
GACCCTAAAA ATGAGACTAA ACAGAAAACA AAAACCATTC GCTCCTCCCT GAATCCAACT 
TGGAACGAAA CGTTTACATT TAAGCTGAAG CCAGCAGATA AGGACCGCCG ACTGGCTGTG 
GAGGTATGGG ACTGGGACAG AACCACCCGC AATGACTTCA TGGGCTCAAT GTCTTTTGGT 
GTGTCGGAGC TCATGAAGTC CCCAGTATGT GGCTGGTACA AAATGTTAAA CCAGGAGGAG 
GGGGAGTACT ATAATGTGCC CATTCCAGAT GAAGATGACC CCGATACTGA ACTCCGACAG 
AAATTTGAGG ATGAGACATT AGAGGACCTT GAGCAGATAG CCAAATTGGG TCCTCCCAAA 
AAAAACATTA CCGCTTTGGA TGACAGTGGG TCCAGCATAA CTTCCAGCAA TGTGGACAAT 
GTCAGGCTCT CAGATTTTAA TTTCCTGGCA GTACTTGGAA AAGGCAGCTT TGGAAAGGTC 
ATGCTGGCAG AGATGAAGTC TTCTGATGAA CTATTTGCTA TAAAAATCCT GAAGAAGGAT 
GTGGTCATCC AGGATGACGA TGTTGAATGC ACCATGGTGG AGAAGCGAGT TCTGGCTCAG 
CAGGACAAGC CTCCATTCCT CACCCAGCTT CATTCCTGCT TCCAGACTGT GGATCGCCTG 
TATTTTGTGA TGGAGTATGT TAATGGTGGT GATCTTATGT ATCACATCCA GCAAGTAGGC 
AAATTTAAGG AGCCCCAGGC TGTGTTCTAT GCTGCAGAGA TTGCTGTTGG GCTCTTCTTC 
TTGCACAAGA AAGGAATTAT ATACAGAGAC CTAAAATTAG ATAATGTTAT GCTGGACGCA 
GAGGGTCATA TTAAGATTGC TGACTTTGGT ATGTGCAAGG AGAACATTGT GGAGGGTGTG 
ACCACCCGTA CATTTTGTGG AACGCCAGAC TACATCGCTC CAGAGATAAT AGCATATCAG 
CCATATGGTC GCTCTGTGGA CTGGTGGGCT TATGGAGTCC TTCTGTATGA GATGCTGGCT 
GGACAACCTC CATTTGATGG TGAGGATGAA GATGAACTTT TCCAGTCTAT TATGGAACAC 
AATGTCTCCT ACTCTAAAAC CTTGTCCAAA GAAGCAGTTT CCATCTGCAA AGGGCTGATG 
ACCAAACACC CTTCAAAACG CCTGGGCTGT GGTCAAGAGG GTGAGAGAGA TATTAGGGAA 
CATGCTTTCT TCCGTCGCAT TGACTGGGAT CGCCTGGCAA ACAGAGAAGT ACAGCCTCCA 
TTCAAACCCA AAGTGTGTGG AAAGGGGGCA GAAAACTTTG ACAAGTTCTT CACTCGCACG 
CAGCCACAAC TGACCCCTCC TGACGAGCTG GTTATTGCCA ACATCGACCA GGCTGATTTC 
GATGGATTTA CCTTCGTCAA TCCTCAATTT GTCCATCCCT CCCTTGTCAA CACTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C9orf72 is essential for neurodevelopment and motility mediated by Cyclin G1.
Experimental neurology304114-124(2018 06)
Yeh TH,Liu HF,Li YW,Lu CS,Shih HY,Chiu CC,Lin SJ,Huang YC,Cheng YC

Phylogeny and distribution of protein kinase C variants in the zebrafish.
The Journal of comparative neurology526(7)1097-1109(2018 05)
Haug MF,Gesemann M,Berger M,Neuhauss SCF

Genetic Reduction in Left Ventricular Protein Kinase C-α and Adverse Ventricular Remodeling in Human Subjects.
Circulation. Genomic and precision medicine11(3)e001901(2018 03)
Hu R,Morley MP,Brandimarto J,Tucker NR,Parsons VA,Zhao SD,Meder B,Katus HA,Rühle F,Stoll M,Villard E,Cambien F,Lin H,Smith NL,Felix JF,Vasan RS,van der Harst P,Newton-Cheh C,Li J,Kim CE,Hakonarson H,Hannenhalli S,Ashley EA,Moravec CS,Tang WHW,Maillet M,Molkentin JD,Ellinor PT,Margulies KB,Cappola TP

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio).
Nanotoxicology10(9)1363-72(2016 11)
Gao J,Mahapatra CT,Mapes CD,Khlebnikova M,Wei A,Sepúlveda MS

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prkca cDNA ORF clone, Danio rerio(zebrafish)

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