Ift140 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift140
Entrez Gene ID	100362124
Full Name	intraflagellar transport 140
General protein information	Preferred Names intraflagellar transport 140 Names intraflagellar transport protein 140 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246054.3	XP_006246116.1	intraflagellar transport protein 140 homolog isoform X1
XM_006220988.3	XP_006221050.1	intraflagellar transport protein 140 homolog isoform X4
XM_002727728.5	XP_002727774.2	intraflagellar transport protein 140 homolog isoform X2
XM_006246055.3	XP_006246117.1	intraflagellar transport protein 140 homolog isoform X3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	IFT140	XP_001089057.1
Bos taurus (cattle)	IFT140	XP_002697959.3
Gallus gallus (chicken)	IFT140	NP_001012810.1
Danio rerio (zebrafish)	ift140	XP_695732.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000686	XP_311215.4
Mus musculus (house mouse)	Ift140	NP_598887.3
Pan troglodytes (chimpanzee)	IFT140	XP_003314958.1
Caenorhabditis elegans (roundworm)	che-11	NP_506047.5
Xenopus tropicalis (tropical clawed frog)	ift140	NP_001116497.1
Homo sapiens (human)	IFT140	NP_055529.2
Drosophila melanogaster (fruit fly)	rempA	NP_995608.1
Rattus norvegicus (Norway rat)	Ift140	XP_006246116.1

Gene Ontology

The following Ift140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa19071	XM_006220988.3 Latest version!	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa32924	XM_006246054.3 Latest version!	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa32925	XM_002727728.5 Latest version!	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa32926	XM_006246055.3 Latest version!	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift140 ( XM_006220988.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006221050.1 Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa19071
Clone ID Related Accession (Same CDS sequence)	XM_006220988.3
Accession Version	XM_006220988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 140 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006220988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTC CCGGCAATAG AGGCCAAAAT ATCAGAAGAA 
GATATTCATC GGCTGAATCA GGAAGACCTG GAGGTCGGAT CAGGTGAGCC CGTGCCTGAC 
ACCCACATTG AGAGGTCCTT CCAGGCCACT TCACTATGCT GGCACCCAAC AAGGCTTATC 
CTGGCTATTG GCTGGGAAAC TGGAGAAGTA ATAATGTTTA ATAAGCAGGA CAAGGAGCAG 
CACACGGTGC CCCTGCCACA TACGACTGAC ATTGCCATCC TCAGTTGGAG TACCAGCGGG 
AGCTGCCTGG TATCTGGAGA CAAGCTCGGG GTCCTGCTCT TGTGGAGATT GGACCAGCGA 
GGCCGAGTAC AGGGGACACC ACTCCTGAAA CACGAATACG GGAAACCGCT CACCCATTGC 
ATCTTCCGGC TCCCCCCTCC TGGGGAGGAT CTGGTTCAGT TGGCAAAGGC AGCAGTAAGT 
GGGGATGAGA AAGCCCTGGA CATGTTTAAC TGGAGAAAAA GCAGCTTTGG AAGTTTCCTG 
AAGACGGGGT CTCAAGAGGG GCTGTCTTTC TTTGTCAGTC TCATGGATGG GACAGTGCAC 
TATGTGGATG AAAAGGGCAA GACTGCTCAG GTGGCATCCA CGGACAGCTC TATCCAGACC 
CTGTTCTACA TTGAGAAGAG AGAGGCACTG GTTGTGGTCA CACAGAACCT TCTCCTGTCT 
CTGTATGTGG TGAATCCTGA GGGGGAAGCT GAAGAAGTCA TGAAGGTAAA GTTGAGTGGG 
AAGACAGGCC GCCGGGCAGA TATCACTCTG ATAGAGGGCA GTCTTCTTGT GACAGCTATT 
GGGGAACCTG TGCTCAGGTT CTGGGACTTA GAACGGGGAG AGAATTACAT ACTGAGTCTA 
CAGGAGAAGT TTGGCTTTGA AAAGGGAGAG AATATAAACT CTGTGTGTTA CTGTAAAGCC 
AAAGGTCTCC TGGCAGCTGG TACCCATAAA GGACGAGTAG CCATGTGGAA GAAAGTGCCA 
GTGTCCCCAA GTGGCCGTGG GGCGGAGGGC AAGGATATGT GGGCCCTTCA GACACCTACT 
GAGCTTGAAG GAAACATCAC ACAGATAAAG TGGGGCTCCA GGAAGAACCT GCTGGCGGTG 
AGCACCTCTG ACTCCGTGTC CATCCTCAGT GAGCAAGCCA TGTCGTCACA CTTCCACCAG 
CAAGTGGCTG CCGTGCAGAT CTCCCCGAGC CTAGTCAACG TGTCTTTCCT GTCCACGGGG 
AGCACGCACA GCCTGCGCAC CGACATGCAC ATCAGTGGAG TGTTTGCCAC CAAGGATGCT 
GTTGCTGTCT GGAATGGAAA ACAGGTGGCG ATCTTTGAAC CTTCAGGGTC CACCTTACGC 
AATGCAGGGA CATTCCTGTG TGAAACATCG GTGCTAGCCA TGCACGAGGA AAGCATTTAC 
ACTGTAGAGC CAAACCGACT CCAAGTCCGC ACCTGGCAGG GCACTGTCAA ACAACTTCTG 
CTGTTCTCTG AGACCGAGGG GAGCCCCTGC TTCCTAGATG TCTGTGGGAC TTTCCTGGTT 
GCAGGGACAG ATTTAGCTCA CTTTAAAAGT TTTGATCTTT CCAGAAGGGA AGCAAAAGTG 
CACTGCAGCT GCAAGAACCT GGCCCAGATG ATCCCCGACG TGGGGAGCAT CACTTCACTG 
AGATGCAATG CCAATGGGAA CAAGATCAGT ATCCTCCTTA GCAAGGTTGA CAACAGTCCT 
GATTCCAAAA TCTACGTCTA TGACGTTGAA ATGGACACAG TGAATGTCTT CAACTTCAAG 
ACTGGACACA TTGGACAGAT ACAGACTCTG CCCTTTAATG AGCCTCCGAC CAATGAGGCC 
CACTTCTTTA TGAATGAGAG CCTAGCAAGT TACGTTCCTG TGAACCATTT CTGGGATCAG 
AGTGAACCCA GGCTCTTTGT GTGCGAGGCC TTGCAGGAGG CACCTGGAGC CCAGCCACAG 
GCTATAGACA AGCAGCCCCA CATGGAGGAA GGCACGTGCC ACAAGGAAGA AGTATTAATT 
CTCTCATTCT TCGCTTCTGA AGAGCATGGC TTCCTGCTTC ATGACAGCTT CCCCCGGCCT 
TCAACCTACC AGAGCCTTCT GGGCCTGGAA GTGCCTCACT ATTACTTCAC AAAAAAGCCT 
GGAGAAGCAG ACAAAGAAGA CAAGATGGAT TCTGGGCACT ACCACATCCC TCAGATGGTA 
GCCAAGAAGC CCCTACGAGA CTTTGTGGGG CTGGAGGACT GTGACAAGCC TACTCGGGAT 
GCCATGCTCA ACTTCAGCTT CTTTGTCACC ATTGGAGACA TGGATGAAGC TTTCAAATCT 
ATCAAACTCA TCAAGAGTGA GACCGTCTGG GAGAACATGG CACGCATGTG TGTGAAGACT 
CAGAGGCTGG ATGTTGCCAA GGTGTGCCTA GGGAACATGG GCCATGCCCG GGGGGCCCGA 
GCACTGCGGG AAGCTGAGCG GGAACCAGAA CTAGAAGCCA GAGTGGCCAT GTTGGCCATA 
CAGCTGGGCA TGCTGGAGGA GGCTGAACAA CTGTATAAGA AGTGCAAACG CTACGACCTC 
CTAAACAAGT TCTACCAGGC CTCAGACCAG TGGCAGAAAG CTGTGGAGGT AGCAGAGCTC 
CATGACCGAG TCCACCTGCG CACCACCTAC TACAACTATG CCAAGCACCT GGAGGCCAGC 
GCCGACTGCG GCCTGGCCCT CAGCTACTAT GAGAAGTCAG ACACACACCG TTTTGAGGTA 
CCTAGGATGC TCTCAGAAGA CCTGCAGTCA CTGGAGCTGT ACATCAATAG GATGAAGGAC 
AAGACCCTGT GGAGATGGTG GGCCCAGTAC CTGGAGAGCC AGGCAGAGAT GGACACCGCG 
TTGCGCTACT ACGAGCTGGC CCAGGACTAC TTCTCCCTGG TCCGTATTCA CTGCTTCCAG 
GGCAACATTC AGAAGGCTGC TGAAATAGCC AACGAGACTG GAGACTGGGC TGCATCCTAC 
CACCTTGCCC GGCAGTACGA GAGCCAGGAT GAGGTAAAAC AGGCAGTGCA CTTCTACACA 
CGGGCACAGG CTTTCAACAA CGCCATCCGC CTGTGCAAGG AGAATGGCTT AGATGACCAA 
CTCATGAACT TGGCCCTCTT GAGCTCCCCC GAGGACATGA TTGAGGCAGC CCGGTATTAC 
GAGGAGAAGG GCGAGCAGAT GGACAGGGCA GTCATGCTGT ATCACAAGGC TGGACACTTC 
TCCAAGGCCC TGGAGCTGGC CTTCACCACC CAGCAGTTTG CAGCCCTACA ACTCATAGCA 
GAGGACCTGG ATGAGAAGTC AGATCCTGCT CTCCTGGCCC GCTGCTCAGA CTTCTGCATT 
GAGCACAGAC AGTTTGAAAA GGCCGTGGAG CTTCTGCTGG CTGCCAAGAA GTACCACGAG 
GCTCTGCAGC TGTGCCTAGA GCAGAACATG ACCATCACTG AGGAGATGGC CGAGAAGATG 
ACTGTGTCCA AGGACTCGAA GGACCTGTCC GAGGAGTCAC GGCGTGAGCT GCTAGAACAG 
ATTGCAAACT GCTGTATGCG CCAGGGCAAC TATCACCTGG CTACCAAGAA GTACACACAG 
GCTGGGAACA AGCTGAAGGC CATGAGAGCC CTGCTCAAAT CTGGAGACAC AGAGAAAATT 
GTGTTCTTTG CGGGTGTCTC AAGACAGAAG GAAATCTACA TCATGGCTGC CAATTACCTA 
CAGTCCTTGG ACTGGCGGAA GGAGCCAGAG ATTATGAAGA ATATCATTAG CTTCTACACA 
AAAGGGCGGG CCTTGGACCT CCTGGCTGGC TTCTATGATG CCTGCGCCCA GGTGGAGATT 
GACGAATACC AGAACTATGA CAAGGCCCAT GGAGCACTGA CTGAGGCCTA TAAGTGCCTG 
TCCAAAGCCA AGGCCAAGAA CCCTCTGGAC CAGGAGACCA AGCTAGCCCA GCTACAGAGC 
AAGATGACAC TAGTGAAGAG GTTCATCCAG GCCCGAAGGA CATACACAGA GGACCCCAAA 
GAATCCGTCA GACAATGCGA GCTGCTCCTA GAAGAGCCAG ACCTAGACAG CACCATTCGT 
GTCGGGGACA TCTATGGCTT CCTGGTGGAG CACCATGTGC AGATGGAGGA ATACCAGATG 
GCCTATAAGT ATCTTGAGGA GATGAGGAAA AGACTTCCTT CTGCCAACAT GTCATACTAT 
GTGGACCAGC GCACTGTGGA CACTGTGCAC CAGGGTCTGG GCCTCCCTCC ATCTCGCATC 
ATGCCTGAGC GGGTCCGCCA CAACAGCATG GAGGACCACA AGGAGGTGTA CGAGGAGGTG 
ATAGAAGAGG TGGACAATGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006221050.1
CDS	847..5253
Translation	MALYFDHRIKAPDASSSPSHITWHPTHPFLPAIEAKISEEDIHRLNQEDLEVGSGEPVPDTHIERSFQATSLCWHPTRLILAIGWETGEVIMFNKQDKEQHTVPLPHTTDIAILSWSTSGSCLVSGDKLGVLLLWRLDQRGRVQGTPLLKHEYGKPLTHCIFRLPPPGEDLVQLAKAAVSGDEKALDMFNWRKSSFGSFLKTGSQEGLSFFVSLMDGTVHYVDEKGKTAQVASTDSSIQTLFYIEKREALVVVTQNLLLSLYVVNPEGEAEEVMKVKLSGKTGRRADITLIEGSLLVTAIGEPVLRFWDLERGENYILSLQEKFGFEKGENINSVCYCKAKGLLAAGTHKGRVAMWKKVPVSPSGRGAEGKDMWALQTPTELEGNITQIKWGSRKNLLAVSTSDSVSILSEQAMSSHFHQQVAAVQISPSLVNVSFLSTGSTHSLRTDMHISGVFATKDAVAVWNGKQVAIFEPSGSTLRNAGTFLCETSVLAMHEESIYTVEPNRLQVRTWQGTVKQLLLFSETEGSPCFLDVCGTFLVAGTDLAHFKSFDLSRREAKVHCSCKNLAQMIPDVGSITSLRCNANGNKISILLSKVDNSPDSKIYVYDVEMDTVNVFNFKTGHIGQIQTLPFNEPPTNEAHFFMNESLASYVPVNHFWDQSEPRLFVCEALQEAPGAQPQAIDKQPHMEEGTCHKEEVLILSFFASEEHGFLLHDSFPRPSTYQSLLGLEVPHYYFTKKPGEADKEDKMDSGHYHIPQMVAKKPLRDFVGLEDCDKPTRDAMLNFSFFVTIGDMDEAFKSIKLIKSETVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAEREPELEARVAMLAIQLGMLEEAEQLYKKCKRYDLLNKFYQASDQWQKAVEVAELHDRVHLRTTYYNYAKHLEASADCGLALSYYEKSDTHRFEVPRMLSEDLQSLELYINRMKDKTLWRWWAQYLESQAEMDTALRYYELAQDYFSLVRIHCFQGNIQKAAEIANETGDWAASYHLARQYESQDEVKQAVHFYTRAQAFNNAIRLCKENGLDDQLMNLALLSSPEDMIEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTQQFAALQLIAEDLDEKSDPALLARCSDFCIEHRQFEKAVELLLAAKKYHEALQLCLEQNMTITEEMAEKMTVSKDSKDLSEESRRELLEQIANCCMRQGNYHLATKKYTQAGNKLKAMRALLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKEPEIMKNIISFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLSKAKAKNPLDQETKLAQLQSKMTLVKRFIQARRTYTEDPKESVRQCELLLEEPDLDSTIRVGDIYGFLVEHHVQMEEYQMAYKYLEEMRKRLPSANMSYYVDQRTVDTVHQGLGLPPSRIMPERVRHNSMEDHKEVYEEVIEEVDNDP

Target ORF information:

RefSeq Version	XM_006220988.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006220988.3

ORF Insert Sequence:

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTC CCGGCAATAG AGGCCAAAAT ATCAGAAGAA 
GATATTCATC GGCTGAATCA GGAAGACCTG GAGGTCGGAT CAGGTGAGCC CGTGCCTGAC 
ACCCACATTG AGAGGTCCTT CCAGGCCACT TCACTATGCT GGCACCCAAC AAGGCTTATC 
CTGGCTATTG GCTGGGAAAC TGGAGAAGTA ATAATGTTTA ATAAGCAGGA CAAGGAGCAG 
CACACGGTGC CCCTGCCACA TACGACTGAC ATTGCCATCC TCAGTTGGAG TACCAGCGGG 
AGCTGCCTGG TATCTGGAGA CAAGCTCGGG GTCCTGCTCT TGTGGAGATT GGACCAGCGA 
GGCCGAGTAC AGGGGACACC ACTCCTGAAA CACGAATACG GGAAACCGCT CACCCATTGC 
ATCTTCCGGC TCCCCCCTCC TGGGGAGGAT CTGGTTCAGT TGGCAAAGGC AGCAGTAAGT 
GGGGATGAGA AAGCCCTGGA CATGTTTAAC TGGAGAAAAA GCAGCTTTGG AAGTTTCCTG 
AAGACGGGGT CTCAAGAGGG GCTGTCTTTC TTTGTCAGTC TCATGGATGG GACAGTGCAC 
TATGTGGATG AAAAGGGCAA GACTGCTCAG GTGGCATCCA CGGACAGCTC TATCCAGACC 
CTGTTCTACA TTGAGAAGAG AGAGGCACTG GTTGTGGTCA CACAGAACCT TCTCCTGTCT 
CTGTATGTGG TGAATCCTGA GGGGGAAGCT GAAGAAGTCA TGAAGGTAAA GTTGAGTGGG 
AAGACAGGCC GCCGGGCAGA TATCACTCTG ATAGAGGGCA GTCTTCTTGT GACAGCTATT 
GGGGAACCTG TGCTCAGGTT CTGGGACTTA GAACGGGGAG AGAATTACAT ACTGAGTCTA 
CAGGAGAAGT TTGGCTTTGA AAAGGGAGAG AATATAAACT CTGTGTGTTA CTGTAAAGCC 
AAAGGTCTCC TGGCAGCTGG TACCCATAAA GGACGAGTAG CCATGTGGAA GAAAGTGCCA 
GTGTCCCCAA GTGGCCGTGG GGCGGAGGGC AAGGATATGT GGGCCCTTCA GACACCTACT 
GAGCTTGAAG GAAACATCAC ACAGATAAAG TGGGGCTCCA GGAAGAACCT GCTGGCGGTG 
AGCACCTCTG ACTCCGTGTC CATCCTCAGT GAGCAAGCCA TGTCGTCACA CTTCCACCAG 
CAAGTGGCTG CCGTGCAGAT CTCCCCGAGC CTAGTCAACG TGTCTTTCCT GTCCACGGGG 
AGCACGCACA GCCTGCGCAC CGACATGCAC ATCAGTGGAG TGTTTGCCAC CAAGGATGCT 
GTTGCTGTCT GGAATGGAAA ACAGGTGGCG ATCTTTGAAC CTTCAGGGTC CACCTTACGC 
AATGCAGGGA CATTCCTGTG TGAAACATCG GTGCTAGCCA TGCACGAGGA AAGCATTTAC 
ACTGTAGAGC CAAACCGACT CCAAGTCCGC ACCTGGCAGG GCACTGTCAA ACAACTTCTG 
CTGTTCTCTG AGACCGAGGG GAGCCCCTGC TTCCTAGATG TCTGTGGGAC TTTCCTGGTT 
GCAGGGACAG ATTTAGCTCA CTTTAAAAGT TTTGATCTTT CCAGAAGGGA AGCAAAAGTG 
CACTGCAGCT GCAAGAACCT GGCCCAGATG ATCCCCGACG TGGGGAGCAT CACTTCACTG 
AGATGCAATG CCAATGGGAA CAAGATCAGT ATCCTCCTTA GCAAGGTTGA CAACAGTCCT 
GATTCCAAAA TCTACGTCTA TGACGTTGAA ATGGACACAG TGAATGTCTT CAACTTCAAG 
ACTGGACACA TTGGACAGAT ACAGACTCTG CCCTTTAATG AGCCTCCGAC CAATGAGGCC 
CACTTCTTTA TGAATGAGAG CCTAGCAAGT TACGTTCCTG TGAACCATTT CTGGGATCAG 
AGTGAACCCA GGCTCTTTGT GTGCGAGGCC TTGCAGGAGG CACCTGGAGC CCAGCCACAG 
GCTATAGACA AGCAGCCCCA CATGGAGGAA GGCACGTGCC ACAAGGAAGA AGTATTAATT 
CTCTCATTCT TCGCTTCTGA AGAGCATGGC TTCCTGCTTC ATGACAGCTT CCCCCGGCCT 
TCAACCTACC AGAGCCTTCT GGGCCTGGAA GTGCCTCACT ATTACTTCAC AAAAAAGCCT 
GGAGAAGCAG ACAAAGAAGA CAAGATGGAT TCTGGGCACT ACCACATCCC TCAGATGGTA 
GCCAAGAAGC CCCTACGAGA CTTTGTGGGG CTGGAGGACT GTGACAAGCC TACTCGGGAT 
GCCATGCTCA ACTTCAGCTT CTTTGTCACC ATTGGAGACA TGGATGAAGC TTTCAAATCT 
ATCAAACTCA TCAAGAGTGA GACCGTCTGG GAGAACATGG CACGCATGTG TGTGAAGACT 
CAGAGGCTGG ATGTTGCCAA GGTGTGCCTA GGGAACATGG GCCATGCCCG GGGGGCCCGA 
GCACTGCGGG AAGCTGAGCG GGAACCAGAA CTAGAAGCCA GAGTGGCCAT GTTGGCCATA 
CAGCTGGGCA TGCTGGAGGA GGCTGAACAA CTGTATAAGA AGTGCAAACG CTACGACCTC 
CTAAACAAGT TCTACCAGGC CTCAGACCAG TGGCAGAAAG CTGTGGAGGT AGCAGAGCTC 
CATGACCGAG TCCACCTGCG CACCACCTAC TACAACTATG CCAAGCACCT GGAGGCCAGC 
GCCGACTGCG GCCTGGCCCT CAGCTACTAT GAGAAGTCAG ACACACACCG TTTTGAGGTA 
CCTAGGATGC TCTCAGAAGA CCTGCAGTCA CTGGAGCTGT ACATCAATAG GATGAAGGAC 
AAGACCCTGT GGAGATGGTG GGCCCAGTAC CTGGAGAGCC AGGCAGAGAT GGACACCGCG 
TTGCGCTACT ACGAGCTGGC CCAGGACTAC TTCTCCCTGG TCCGTATTCA CTGCTTCCAG 
GGCAACATTC AGAAGGCTGC TGAAATAGCC AACGAGACTG GAGACTGGGC TGCATCCTAC 
CACCTTGCCC GGCAGTACGA GAGCCAGGAT GAGGTAAAAC AGGCAGTGCA CTTCTACACA 
CGGGCACAGG CTTTCAACAA CGCCATCCGC CTGTGCAAGG AGAATGGCTT AGATGACCAA 
CTCATGAACT TGGCCCTCTT GAGCTCCCCC GAGGACATGA TTGAGGCAGC CCGGTATTAC 
GAGGAGAAGG GCGAGCAGAT GGACAGGGCA GTCATGCTGT ATCACAAGGC TGGACACTTC 
TCCAAGGCCC TGGAGCTGGC CTTCACCACC CAGCAGTTTG CAGCCCTACA ACTCATAGCA 
GAGGACCTGG ATGAGAAGTC AGATCCTGCT CTCCTGGCCC GCTGCTCAGA CTTCTGCATT 
GAGCACAGAC AGTTTGAAAA GGCCGTGGAG CTTCTGCTGG CTGCCAAGAA GTACCACGAG 
GCTCTGCAGC TGTGCCTAGA GCAGAACATG ACCATCACTG AGGAGATGGC CGAGAAGATG 
ACTGTGTCCA AGGACTCGAA GGACCTGTCC GAGGAGTCAC GGCGTGAGCT GCTAGAACAG 
ATTGCAAACT GCTGTATGCG CCAGGGCAAC TATCACCTGG CTACCAAGAA GTACACACAG 
GCTGGGAACA AGCTGAAGGC CATGAGAGCC CTGCTCAAAT CTGGAGACAC AGAGAAAATT 
GTGTTCTTTG CGGGTGTCTC AAGACAGAAG GAAATCTACA TCATGGCTGC CAATTACCTA 
CAGTCCTTGG ACTGGCGGAA GGAGCCAGAG ATTATGAAGA ATATCATTAG CTTCTACACA 
AAAGGGCGGG CCTTGGACCT CCTGGCTGGC TTCTATGATG CCTGCGCCCA GGTGGAGATT 
GACGAATACC AGAACTATGA CAAGGCCCAT GGAGCACTGA CTGAGGCCTA TAAGTGCCTG 
TCCAAAGCCA AGGCCAAGAA CCCTCTGGAC CAGGAGACCA AGCTAGCCCA GCTACAGAGC 
AAGATGACAC TAGTGAAGAG GTTCATCCAG GCCCGAAGGA CATACACAGA GGACCCCAAA 
GAATCCGTCA GACAATGCGA GCTGCTCCTA GAAGAGCCAG ACCTAGACAG CACCATTCGT 
GTCGGGGACA TCTATGGCTT CCTGGTGGAG CACCATGTGC AGATGGAGGA ATACCAGATG 
GCCTATAAGT ATCTTGAGGA GATGAGGAAA AGACTTCCTT CTGCCAACAT GTCATACTAT 
GTGGACCAGC GCACTGTGGA CACTGTGCAC CAGGGTCTGG GCCTCCCTCC ATCTCGCATC 
ATGCCTGAGC GGGTCCGCCA CAACAGCATG GAGGACCACA AGGAGGTGTA CGAGGAGGTG 
ATAGAAGAGG TGGACAATGA CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift140 ( XM_006246054.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246116.1 Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32924
Clone ID Related Accession (Same CDS sequence)	XM_006246054.3
Accession Version	XM_006246054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG GAAGAAGTAT TAATTCTCTC ATTCTTCGCT 
TCTGAAGAGC ATGGCTTCCT GCTTCATGAC AGCTTCCCCC GGCCTTCAAC CTACCAGAGC 
CTTCTGGGCC TGGAAGTGCC TCACTATTAC TTCACAAAAA AGTTGTTGTT CCACAGAAGA 
GGCGTCTATG ATGCGGTCCC TGGAGAAGCA GACAAAGAAG ACAAGATGGA TTCTGGGCAC 
TACCACATCC CTCAGATGGT AGCCAAGAAG CCCCTACGAG ACTTTGTGGG GCTGGAGGAC 
TGTGACAAGC CTACTCGGGA TGCCATGCTC AACTTCAGCT TCTTTGTCAC CATTGGAGAC 
ATGGATGAAG CTTTCAAATC TATCAAACTC ATCAAGAGTG AGACCGTCTG GGAGAACATG 
GCACGCATGT GTGTGAAGAC TCAGAGGCTG GATGTTGCCA AGGTGTGCCT AGGGAACATG 
GGCCATGCCC GGGGGGCCCG AGCACTGCGG GAAGCTGAGC GGGAACCAGA ACTAGAAGCC 
AGAGTGGCCA TGTTGGCCAT ACAGCTGGGC ATGCTGGAGG AGGCTGAACA ACTGTATAAG 
AAGTGCAAAC GCTACGACCT CCTAAACAAG TTCTACCAGG CCTCAGACCA GTGGCAGAAA 
GCTGTGGAGG TAGCAGAGCT CCATGACCGA GTCCACCTGC GCACCACCTA CTACAACTAT 
GCCAAGCACC TGGAGGCCAG CGCCGACTGC GGCCTGGCCC TCAGCTACTA TGAGAAGTCA 
GACACACACC GTTTTGAGGT ACCTAGGATG CTCTCAGAAG ACCTGCAGTC ACTGGAGCTG 
TACATCAATA GGATGAAGGA CAAGACCCTG TGGAGATGGT GGGCCCAGTA CCTGGAGAGC 
CAGGCAGAGA TGGACACCGC GTTGCGCTAC TACGAGCTGG CCCAGGACTA CTTCTCCCTG 
GTCCGTATTC ACTGCTTCCA GGGCAACATT CAGAAGGCTG CTGAAATAGC CAACGAGACT 
GGAGACTGGG CTGCATCCTA CCACCTTGCC CGGCAGTACG AGAGCCAGGA TGAGGTAAAA 
CAGGCAGTGC ACTTCTACAC ACGGGCACAG GCTTTCAACA ACGCCATCCG CCTGTGCAAG 
GAGAATGGCT TAGATGACCA ACTCATGAAC TTGGCCCTCT TGAGCTCCCC CGAGGACATG 
ATTGAGGCAG CCCGGTATTA CGAGGAGAAG GGCGAGCAGA TGGACAGGGC AGTCATGCTG 
TATCACAAGG CTGGACACTT CTCCAAGGCC CTGGAGCTGG CCTTCACCAC CCAGCAGTTT 
GCAGCCCTAC AACTCATAGC AGAGGACCTG GATGAGAAGT CAGATCCTGC TCTCCTGGCC 
CGCTGCTCAG ACTTCTGCAT TGAGCACAGA CAGTTTGAAA AGGCCGTGGA GCTTCTGCTG 
GCTGCCAAGA AGTACCACGA GGCTCTGCAG CTGTGCCTAG AGCAGAACAT GACCATCACT 
GAGGAGATGG CCGAGAAGAT GACTGTGTCC AAGGACTCGA AGGACCTGTC CGAGGAGTCA 
CGGCGTGAGC TGCTAGAACA GATTGCAAAC TGCTGTATGC GCCAGGGCAA CTATCACCTG 
GCTACCAAGA AGTACACACA GGCTGGGAAC AAGCTGAAGG CCATGAGAGC CCTGCTCAAA 
TCTGGAGACA CAGAGAAAAT TGTGTTCTTT GCGGGTGTCT CAAGACAGAA GGAAATCTAC 
ATCATGGCTG CCAATTACCT ACAGTCCTTG GACTGGCGGA AGGAGCCAGA GATTATGAAG 
AATATCATTA GCTTCTACAC AAAAGGGCGG GCCTTGGACC TCCTGGCTGG CTTCTATGAT 
GCCTGCGCCC AGGTGGAGAT TGACGAATAC CAGAACTATG ACAAGGCCCA TGGAGCACTG 
ACTGAGGCCT ATAAGTGCCT GTCCAAAGCC AAGGCCAAGA ACCCTCTGGA CCAGGAGACC 
AAGCTAGCCC AGCTACAGAG CAAGATGACA CTAGTGAAGA GGTTCATCCA GGCCCGAAGG 
ACATACACAG AGGACCCCAA AGAATCCGTC AGACAATGCG AGCTGCTCCT AGAAGAGCCA 
GACCTAGACA GCACCATTCG TGTCGGGGAC ATCTATGGCT TCCTGGTGGA GCACCATGTG 
CAGATGGAGG AATACCAGAT GGCCTATAAG TATCTTGAGG AGATGAGGAA AAGACTTCCT 
TCTGCCAACA TGTCATACTA TGTGGACCAG CGCACTGTGG ACACTGTGCA CCAGGGTCTG 
GGCCTCCCTC CATCTCGCAT CATGCCTGAG CGGGTCCGCC ACAACAGCAT GGAGGACCAC 
AAGGAGGTGT ACGAGGAGGT GATAGAAGAG GTGGACAATG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246116.1
CDS	846..5273
Translation	MALYFDHRIKAPDASSSPSHITWHPTHPFLAVASVSPSSGGNVDIYLEQGEPVPDTHIERSFQATSLCWHPTRLILAIGWETGEVIMFNKQDKEQHTVPLPHTTDIAILSWSTSGSCLVSGDKLGVLLLWRLDQRGRVQGTPLLKHEYGKPLTHCIFRLPPPGEDLVQLAKAAVSGDEKALDMFNWRKSSFGSFLKTGSQEGLSFFVSLMDGTVHYVDEKGKTAQVASTDSSIQTLFYIEKREALVVVTQNLLLSLYVVNPEGEAEEVMKVKLSGKTGRRADITLIEGSLLVTAIGEPVLRFWDLERGENYILSLQEKFGFEKGENINSVCYCKAKGLLAAGTHKGRVAMWKKVPVSPSGRGAEGKDMWALQTPTELEGNITQIKWGSRKNLLAVSTSDSVSILSEQAMSSHFHQQVAAVQISPSLVNVSFLSTGSTHSLRTDMHISGVFATKDAVAVWNGKQVAIFEPSGSTLRNAGTFLCETSVLAMHEESIYTVEPNRLQVRTWQGTVKQLLLFSETEGSPCFLDVCGTFLVAGTDLAHFKSFDLSRREAKVHCSCKNLAQMIPDVGSITSLRCNANGNKISILLSKVDNSPDSKIYVYDVEMDTVNVFNFKTGHIGQIQTLPFNEPPTNEAHFFMNESLASYVPVNHFWDQSEPRLFVCEALQEAPGAQPQAIDKQPHMEEGTCHKEEVLILSFFASEEHGFLLHDSFPRPSTYQSLLGLEVPHYYFTKKLLFHRRGVYDAVPGEADKEDKMDSGHYHIPQMVAKKPLRDFVGLEDCDKPTRDAMLNFSFFVTIGDMDEAFKSIKLIKSETVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAEREPELEARVAMLAIQLGMLEEAEQLYKKCKRYDLLNKFYQASDQWQKAVEVAELHDRVHLRTTYYNYAKHLEASADCGLALSYYEKSDTHRFEVPRMLSEDLQSLELYINRMKDKTLWRWWAQYLESQAEMDTALRYYELAQDYFSLVRIHCFQGNIQKAAEIANETGDWAASYHLARQYESQDEVKQAVHFYTRAQAFNNAIRLCKENGLDDQLMNLALLSSPEDMIEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTQQFAALQLIAEDLDEKSDPALLARCSDFCIEHRQFEKAVELLLAAKKYHEALQLCLEQNMTITEEMAEKMTVSKDSKDLSEESRRELLEQIANCCMRQGNYHLATKKYTQAGNKLKAMRALLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKEPEIMKNIISFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLSKAKAKNPLDQETKLAQLQSKMTLVKRFIQARRTYTEDPKESVRQCELLLEEPDLDSTIRVGDIYGFLVEHHVQMEEYQMAYKYLEEMRKRLPSANMSYYVDQRTVDTVHQGLGLPPSRIMPERVRHNSMEDHKEVYEEVIEEVDNDP

Target ORF information:

RefSeq Version	XM_006246054.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246054.3

ORF Insert Sequence:

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG GAAGAAGTAT TAATTCTCTC ATTCTTCGCT 
TCTGAAGAGC ATGGCTTCCT GCTTCATGAC AGCTTCCCCC GGCCTTCAAC CTACCAGAGC 
CTTCTGGGCC TGGAAGTGCC TCACTATTAC TTCACAAAAA AGTTGTTGTT CCACAGAAGA 
GGCGTCTATG ATGCGGTCCC TGGAGAAGCA GACAAAGAAG ACAAGATGGA TTCTGGGCAC 
TACCACATCC CTCAGATGGT AGCCAAGAAG CCCCTACGAG ACTTTGTGGG GCTGGAGGAC 
TGTGACAAGC CTACTCGGGA TGCCATGCTC AACTTCAGCT TCTTTGTCAC CATTGGAGAC 
ATGGATGAAG CTTTCAAATC TATCAAACTC ATCAAGAGTG AGACCGTCTG GGAGAACATG 
GCACGCATGT GTGTGAAGAC TCAGAGGCTG GATGTTGCCA AGGTGTGCCT AGGGAACATG 
GGCCATGCCC GGGGGGCCCG AGCACTGCGG GAAGCTGAGC GGGAACCAGA ACTAGAAGCC 
AGAGTGGCCA TGTTGGCCAT ACAGCTGGGC ATGCTGGAGG AGGCTGAACA ACTGTATAAG 
AAGTGCAAAC GCTACGACCT CCTAAACAAG TTCTACCAGG CCTCAGACCA GTGGCAGAAA 
GCTGTGGAGG TAGCAGAGCT CCATGACCGA GTCCACCTGC GCACCACCTA CTACAACTAT 
GCCAAGCACC TGGAGGCCAG CGCCGACTGC GGCCTGGCCC TCAGCTACTA TGAGAAGTCA 
GACACACACC GTTTTGAGGT ACCTAGGATG CTCTCAGAAG ACCTGCAGTC ACTGGAGCTG 
TACATCAATA GGATGAAGGA CAAGACCCTG TGGAGATGGT GGGCCCAGTA CCTGGAGAGC 
CAGGCAGAGA TGGACACCGC GTTGCGCTAC TACGAGCTGG CCCAGGACTA CTTCTCCCTG 
GTCCGTATTC ACTGCTTCCA GGGCAACATT CAGAAGGCTG CTGAAATAGC CAACGAGACT 
GGAGACTGGG CTGCATCCTA CCACCTTGCC CGGCAGTACG AGAGCCAGGA TGAGGTAAAA 
CAGGCAGTGC ACTTCTACAC ACGGGCACAG GCTTTCAACA ACGCCATCCG CCTGTGCAAG 
GAGAATGGCT TAGATGACCA ACTCATGAAC TTGGCCCTCT TGAGCTCCCC CGAGGACATG 
ATTGAGGCAG CCCGGTATTA CGAGGAGAAG GGCGAGCAGA TGGACAGGGC AGTCATGCTG 
TATCACAAGG CTGGACACTT CTCCAAGGCC CTGGAGCTGG CCTTCACCAC CCAGCAGTTT 
GCAGCCCTAC AACTCATAGC AGAGGACCTG GATGAGAAGT CAGATCCTGC TCTCCTGGCC 
CGCTGCTCAG ACTTCTGCAT TGAGCACAGA CAGTTTGAAA AGGCCGTGGA GCTTCTGCTG 
GCTGCCAAGA AGTACCACGA GGCTCTGCAG CTGTGCCTAG AGCAGAACAT GACCATCACT 
GAGGAGATGG CCGAGAAGAT GACTGTGTCC AAGGACTCGA AGGACCTGTC CGAGGAGTCA 
CGGCGTGAGC TGCTAGAACA GATTGCAAAC TGCTGTATGC GCCAGGGCAA CTATCACCTG 
GCTACCAAGA AGTACACACA GGCTGGGAAC AAGCTGAAGG CCATGAGAGC CCTGCTCAAA 
TCTGGAGACA CAGAGAAAAT TGTGTTCTTT GCGGGTGTCT CAAGACAGAA GGAAATCTAC 
ATCATGGCTG CCAATTACCT ACAGTCCTTG GACTGGCGGA AGGAGCCAGA GATTATGAAG 
AATATCATTA GCTTCTACAC AAAAGGGCGG GCCTTGGACC TCCTGGCTGG CTTCTATGAT 
GCCTGCGCCC AGGTGGAGAT TGACGAATAC CAGAACTATG ACAAGGCCCA TGGAGCACTG 
ACTGAGGCCT ATAAGTGCCT GTCCAAAGCC AAGGCCAAGA ACCCTCTGGA CCAGGAGACC 
AAGCTAGCCC AGCTACAGAG CAAGATGACA CTAGTGAAGA GGTTCATCCA GGCCCGAAGG 
ACATACACAG AGGACCCCAA AGAATCCGTC AGACAATGCG AGCTGCTCCT AGAAGAGCCA 
GACCTAGACA GCACCATTCG TGTCGGGGAC ATCTATGGCT TCCTGGTGGA GCACCATGTG 
CAGATGGAGG AATACCAGAT GGCCTATAAG TATCTTGAGG AGATGAGGAA AAGACTTCCT 
TCTGCCAACA TGTCATACTA TGTGGACCAG CGCACTGTGG ACACTGTGCA CCAGGGTCTG 
GGCCTCCCTC CATCTCGCAT CATGCCTGAG CGGGTCCGCC ACAACAGCAT GGAGGACCAC 
AAGGAGGTGT ACGAGGAGGT GATAGAAGAG GTGGACAATG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift140 ( XM_002727728.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002727774.2 Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X2, mRNA.

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CloneID	ORa32925
Clone ID Related Accession (Same CDS sequence)	XM_002727728.5
Accession Version	XM_002727728.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 140 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002727728.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG GAAGAAGTAT TAATTCTCTC ATTCTTCGCT 
TCTGAAGAGC ATGGCTTCCT GCTTCATGAC AGCTTCCCCC GGCCTTCAAC CTACCAGAGC 
CTTCTGGGCC TGGAAGTGCC TCACTATTAC TTCACAAAAA AGCCTGGAGA AGCAGACAAA 
GAAGACAAGA TGGATTCTGG GCACTACCAC ATCCCTCAGA TGGTAGCCAA GAAGCCCCTA 
CGAGACTTTG TGGGGCTGGA GGACTGTGAC AAGCCTACTC GGGATGCCAT GCTCAACTTC 
AGCTTCTTTG TCACCATTGG AGACATGGAT GAAGCTTTCA AATCTATCAA ACTCATCAAG 
AGTGAGACCG TCTGGGAGAA CATGGCACGC ATGTGTGTGA AGACTCAGAG GCTGGATGTT 
GCCAAGGTGT GCCTAGGGAA CATGGGCCAT GCCCGGGGGG CCCGAGCACT GCGGGAAGCT 
GAGCGGGAAC CAGAACTAGA AGCCAGAGTG GCCATGTTGG CCATACAGCT GGGCATGCTG 
GAGGAGGCTG AACAACTGTA TAAGAAGTGC AAACGCTACG ACCTCCTAAA CAAGTTCTAC 
CAGGCCTCAG ACCAGTGGCA GAAAGCTGTG GAGGTAGCAG AGCTCCATGA CCGAGTCCAC 
CTGCGCACCA CCTACTACAA CTATGCCAAG CACCTGGAGG CCAGCGCCGA CTGCGGCCTG 
GCCCTCAGCT ACTATGAGAA GTCAGACACA CACCGTTTTG AGGTACCTAG GATGCTCTCA 
GAAGACCTGC AGTCACTGGA GCTGTACATC AATAGGATGA AGGACAAGAC CCTGTGGAGA 
TGGTGGGCCC AGTACCTGGA GAGCCAGGCA GAGATGGACA CCGCGTTGCG CTACTACGAG 
CTGGCCCAGG ACTACTTCTC CCTGGTCCGT ATTCACTGCT TCCAGGGCAA CATTCAGAAG 
GCTGCTGAAA TAGCCAACGA GACTGGAGAC TGGGCTGCAT CCTACCACCT TGCCCGGCAG 
TACGAGAGCC AGGATGAGGT AAAACAGGCA GTGCACTTCT ACACACGGGC ACAGGCTTTC 
AACAACGCCA TCCGCCTGTG CAAGGAGAAT GGCTTAGATG ACCAACTCAT GAACTTGGCC 
CTCTTGAGCT CCCCCGAGGA CATGATTGAG GCAGCCCGGT ATTACGAGGA GAAGGGCGAG 
CAGATGGACA GGGCAGTCAT GCTGTATCAC AAGGCTGGAC ACTTCTCCAA GGCCCTGGAG 
CTGGCCTTCA CCACCCAGCA GTTTGCAGCC CTACAACTCA TAGCAGAGGA CCTGGATGAG 
AAGTCAGATC CTGCTCTCCT GGCCCGCTGC TCAGACTTCT GCATTGAGCA CAGACAGTTT 
GAAAAGGCCG TGGAGCTTCT GCTGGCTGCC AAGAAGTACC ACGAGGCTCT GCAGCTGTGC 
CTAGAGCAGA ACATGACCAT CACTGAGGAG ATGGCCGAGA AGATGACTGT GTCCAAGGAC 
TCGAAGGACC TGTCCGAGGA GTCACGGCGT GAGCTGCTAG AACAGATTGC AAACTGCTGT 
ATGCGCCAGG GCAACTATCA CCTGGCTACC AAGAAGTACA CACAGGCTGG GAACAAGCTG 
AAGGCCATGA GAGCCCTGCT CAAATCTGGA GACACAGAGA AAATTGTGTT CTTTGCGGGT 
GTCTCAAGAC AGAAGGAAAT CTACATCATG GCTGCCAATT ACCTACAGTC CTTGGACTGG 
CGGAAGGAGC CAGAGATTAT GAAGAATATC ATTAGCTTCT ACACAAAAGG GCGGGCCTTG 
GACCTCCTGG CTGGCTTCTA TGATGCCTGC GCCCAGGTGG AGATTGACGA ATACCAGAAC 
TATGACAAGG CCCATGGAGC ACTGACTGAG GCCTATAAGT GCCTGTCCAA AGCCAAGGCC 
AAGAACCCTC TGGACCAGGA GACCAAGCTA GCCCAGCTAC AGAGCAAGAT GACACTAGTG 
AAGAGGTTCA TCCAGGCCCG AAGGACATAC ACAGAGGACC CCAAAGAATC CGTCAGACAA 
TGCGAGCTGC TCCTAGAAGA GCCAGACCTA GACAGCACCA TTCGTGTCGG GGACATCTAT 
GGCTTCCTGG TGGAGCACCA TGTGCAGATG GAGGAATACC AGATGGCCTA TAAGTATCTT 
GAGGAGATGA GGAAAAGACT TCCTTCTGCC AACATGTCAT ACTATGTGGA CCAGCGCACT 
GTGGACACTG TGCACCAGGG TCTGGGCCTC CCTCCATCTC GCATCATGCC TGAGCGGGTC 
CGCCACAACA GCATGGAGGA CCACAAGGAG GTGTACGAGG AGGTGATAGA AGAGGTGGAC 
AATGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002727774.2
CDS	845..5236
Translation	MALYFDHRIKAPDASSSPSHITWHPTHPFLAVASVSPSSGGNVDIYLEQGEPVPDTHIERSFQATSLCWHPTRLILAIGWETGEVIMFNKQDKEQHTVPLPHTTDIAILSWSTSGSCLVSGDKLGVLLLWRLDQRGRVQGTPLLKHEYGKPLTHCIFRLPPPGEDLVQLAKAAVSGDEKALDMFNWRKSSFGSFLKTGSQEGLSFFVSLMDGTVHYVDEKGKTAQVASTDSSIQTLFYIEKREALVVVTQNLLLSLYVVNPEGEAEEVMKVKLSGKTGRRADITLIEGSLLVTAIGEPVLRFWDLERGENYILSLQEKFGFEKGENINSVCYCKAKGLLAAGTHKGRVAMWKKVPVSPSGRGAEGKDMWALQTPTELEGNITQIKWGSRKNLLAVSTSDSVSILSEQAMSSHFHQQVAAVQISPSLVNVSFLSTGSTHSLRTDMHISGVFATKDAVAVWNGKQVAIFEPSGSTLRNAGTFLCETSVLAMHEESIYTVEPNRLQVRTWQGTVKQLLLFSETEGSPCFLDVCGTFLVAGTDLAHFKSFDLSRREAKVHCSCKNLAQMIPDVGSITSLRCNANGNKISILLSKVDNSPDSKIYVYDVEMDTVNVFNFKTGHIGQIQTLPFNEPPTNEAHFFMNESLASYVPVNHFWDQSEPRLFVCEALQEAPGAQPQAIDKQPHMEEGTCHKEEVLILSFFASEEHGFLLHDSFPRPSTYQSLLGLEVPHYYFTKKPGEADKEDKMDSGHYHIPQMVAKKPLRDFVGLEDCDKPTRDAMLNFSFFVTIGDMDEAFKSIKLIKSETVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAEREPELEARVAMLAIQLGMLEEAEQLYKKCKRYDLLNKFYQASDQWQKAVEVAELHDRVHLRTTYYNYAKHLEASADCGLALSYYEKSDTHRFEVPRMLSEDLQSLELYINRMKDKTLWRWWAQYLESQAEMDTALRYYELAQDYFSLVRIHCFQGNIQKAAEIANETGDWAASYHLARQYESQDEVKQAVHFYTRAQAFNNAIRLCKENGLDDQLMNLALLSSPEDMIEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTQQFAALQLIAEDLDEKSDPALLARCSDFCIEHRQFEKAVELLLAAKKYHEALQLCLEQNMTITEEMAEKMTVSKDSKDLSEESRRELLEQIANCCMRQGNYHLATKKYTQAGNKLKAMRALLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKEPEIMKNIISFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLSKAKAKNPLDQETKLAQLQSKMTLVKRFIQARRTYTEDPKESVRQCELLLEEPDLDSTIRVGDIYGFLVEHHVQMEEYQMAYKYLEEMRKRLPSANMSYYVDQRTVDTVHQGLGLPPSRIMPERVRHNSMEDHKEVYEEVIEEVDNDP

Target ORF information:

RefSeq Version	XM_002727728.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002727728.5

ORF Insert Sequence:

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG GAAGAAGTAT TAATTCTCTC ATTCTTCGCT 
TCTGAAGAGC ATGGCTTCCT GCTTCATGAC AGCTTCCCCC GGCCTTCAAC CTACCAGAGC 
CTTCTGGGCC TGGAAGTGCC TCACTATTAC TTCACAAAAA AGCCTGGAGA AGCAGACAAA 
GAAGACAAGA TGGATTCTGG GCACTACCAC ATCCCTCAGA TGGTAGCCAA GAAGCCCCTA 
CGAGACTTTG TGGGGCTGGA GGACTGTGAC AAGCCTACTC GGGATGCCAT GCTCAACTTC 
AGCTTCTTTG TCACCATTGG AGACATGGAT GAAGCTTTCA AATCTATCAA ACTCATCAAG 
AGTGAGACCG TCTGGGAGAA CATGGCACGC ATGTGTGTGA AGACTCAGAG GCTGGATGTT 
GCCAAGGTGT GCCTAGGGAA CATGGGCCAT GCCCGGGGGG CCCGAGCACT GCGGGAAGCT 
GAGCGGGAAC CAGAACTAGA AGCCAGAGTG GCCATGTTGG CCATACAGCT GGGCATGCTG 
GAGGAGGCTG AACAACTGTA TAAGAAGTGC AAACGCTACG ACCTCCTAAA CAAGTTCTAC 
CAGGCCTCAG ACCAGTGGCA GAAAGCTGTG GAGGTAGCAG AGCTCCATGA CCGAGTCCAC 
CTGCGCACCA CCTACTACAA CTATGCCAAG CACCTGGAGG CCAGCGCCGA CTGCGGCCTG 
GCCCTCAGCT ACTATGAGAA GTCAGACACA CACCGTTTTG AGGTACCTAG GATGCTCTCA 
GAAGACCTGC AGTCACTGGA GCTGTACATC AATAGGATGA AGGACAAGAC CCTGTGGAGA 
TGGTGGGCCC AGTACCTGGA GAGCCAGGCA GAGATGGACA CCGCGTTGCG CTACTACGAG 
CTGGCCCAGG ACTACTTCTC CCTGGTCCGT ATTCACTGCT TCCAGGGCAA CATTCAGAAG 
GCTGCTGAAA TAGCCAACGA GACTGGAGAC TGGGCTGCAT CCTACCACCT TGCCCGGCAG 
TACGAGAGCC AGGATGAGGT AAAACAGGCA GTGCACTTCT ACACACGGGC ACAGGCTTTC 
AACAACGCCA TCCGCCTGTG CAAGGAGAAT GGCTTAGATG ACCAACTCAT GAACTTGGCC 
CTCTTGAGCT CCCCCGAGGA CATGATTGAG GCAGCCCGGT ATTACGAGGA GAAGGGCGAG 
CAGATGGACA GGGCAGTCAT GCTGTATCAC AAGGCTGGAC ACTTCTCCAA GGCCCTGGAG 
CTGGCCTTCA CCACCCAGCA GTTTGCAGCC CTACAACTCA TAGCAGAGGA CCTGGATGAG 
AAGTCAGATC CTGCTCTCCT GGCCCGCTGC TCAGACTTCT GCATTGAGCA CAGACAGTTT 
GAAAAGGCCG TGGAGCTTCT GCTGGCTGCC AAGAAGTACC ACGAGGCTCT GCAGCTGTGC 
CTAGAGCAGA ACATGACCAT CACTGAGGAG ATGGCCGAGA AGATGACTGT GTCCAAGGAC 
TCGAAGGACC TGTCCGAGGA GTCACGGCGT GAGCTGCTAG AACAGATTGC AAACTGCTGT 
ATGCGCCAGG GCAACTATCA CCTGGCTACC AAGAAGTACA CACAGGCTGG GAACAAGCTG 
AAGGCCATGA GAGCCCTGCT CAAATCTGGA GACACAGAGA AAATTGTGTT CTTTGCGGGT 
GTCTCAAGAC AGAAGGAAAT CTACATCATG GCTGCCAATT ACCTACAGTC CTTGGACTGG 
CGGAAGGAGC CAGAGATTAT GAAGAATATC ATTAGCTTCT ACACAAAAGG GCGGGCCTTG 
GACCTCCTGG CTGGCTTCTA TGATGCCTGC GCCCAGGTGG AGATTGACGA ATACCAGAAC 
TATGACAAGG CCCATGGAGC ACTGACTGAG GCCTATAAGT GCCTGTCCAA AGCCAAGGCC 
AAGAACCCTC TGGACCAGGA GACCAAGCTA GCCCAGCTAC AGAGCAAGAT GACACTAGTG 
AAGAGGTTCA TCCAGGCCCG AAGGACATAC ACAGAGGACC CCAAAGAATC CGTCAGACAA 
TGCGAGCTGC TCCTAGAAGA GCCAGACCTA GACAGCACCA TTCGTGTCGG GGACATCTAT 
GGCTTCCTGG TGGAGCACCA TGTGCAGATG GAGGAATACC AGATGGCCTA TAAGTATCTT 
GAGGAGATGA GGAAAAGACT TCCTTCTGCC AACATGTCAT ACTATGTGGA CCAGCGCACT 
GTGGACACTG TGCACCAGGG TCTGGGCCTC CCTCCATCTC GCATCATGCC TGAGCGGGTC 
CGCCACAACA GCATGGAGGA CCACAAGGAG GTGTACGAGG AGGTGATAGA AGAGGTGGAC 
AATGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift140 ( XM_006246055.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246117.1 Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X3, mRNA.

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CloneID	ORa32926
Clone ID Related Accession (Same CDS sequence)	XM_006246055.3
Accession Version	XM_006246055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 140 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG CCTGGAGAAG CAGACAAAGA AGACAAGATG 
GATTCTGGGC ACTACCACAT CCCTCAGATG GTAGCCAAGA AGCCCCTACG AGACTTTGTG 
GGGCTGGAGG ACTGTGACAA GCCTACTCGG GATGCCATGC TCAACTTCAG CTTCTTTGTC 
ACCATTGGAG ACATGGATGA AGCTTTCAAA TCTATCAAAC TCATCAAGAG TGAGACCGTC 
TGGGAGAACA TGGCACGCAT GTGTGTGAAG ACTCAGAGGC TGGATGTTGC CAAGGTGTGC 
CTAGGGAACA TGGGCCATGC CCGGGGGGCC CGAGCACTGC GGGAAGCTGA GCGGGAACCA 
GAACTAGAAG CCAGAGTGGC CATGTTGGCC ATACAGCTGG GCATGCTGGA GGAGGCTGAA 
CAACTGTATA AGAAGTGCAA ACGCTACGAC CTCCTAAACA AGTTCTACCA GGCCTCAGAC 
CAGTGGCAGA AAGCTGTGGA GGTAGCAGAG CTCCATGACC GAGTCCACCT GCGCACCACC 
TACTACAACT ATGCCAAGCA CCTGGAGGCC AGCGCCGACT GCGGCCTGGC CCTCAGCTAC 
TATGAGAAGT CAGACACACA CCGTTTTGAG GTACCTAGGA TGCTCTCAGA AGACCTGCAG 
TCACTGGAGC TGTACATCAA TAGGATGAAG GACAAGACCC TGTGGAGATG GTGGGCCCAG 
TACCTGGAGA GCCAGGCAGA GATGGACACC GCGTTGCGCT ACTACGAGCT GGCCCAGGAC 
TACTTCTCCC TGGTCCGTAT TCACTGCTTC CAGGGCAACA TTCAGAAGGC TGCTGAAATA 
GCCAACGAGA CTGGAGACTG GGCTGCATCC TACCACCTTG CCCGGCAGTA CGAGAGCCAG 
GATGAGGTAA AACAGGCAGT GCACTTCTAC ACACGGGCAC AGGCTTTCAA CAACGCCATC 
CGCCTGTGCA AGGAGAATGG CTTAGATGAC CAACTCATGA ACTTGGCCCT CTTGAGCTCC 
CCCGAGGACA TGATTGAGGC AGCCCGGTAT TACGAGGAGA AGGGCGAGCA GATGGACAGG 
GCAGTCATGC TGTATCACAA GGCTGGACAC TTCTCCAAGG CCCTGGAGCT GGCCTTCACC 
ACCCAGCAGT TTGCAGCCCT ACAACTCATA GCAGAGGACC TGGATGAGAA GTCAGATCCT 
GCTCTCCTGG CCCGCTGCTC AGACTTCTGC ATTGAGCACA GACAGTTTGA AAAGGCCGTG 
GAGCTTCTGC TGGCTGCCAA GAAGTACCAC GAGGCTCTGC AGCTGTGCCT AGAGCAGAAC 
ATGACCATCA CTGAGGAGAT GGCCGAGAAG ATGACTGTGT CCAAGGACTC GAAGGACCTG 
TCCGAGGAGT CACGGCGTGA GCTGCTAGAA CAGATTGCAA ACTGCTGTAT GCGCCAGGGC 
AACTATCACC TGGCTACCAA GAAGTACACA CAGGCTGGGA ACAAGCTGAA GGCCATGAGA 
GCCCTGCTCA AATCTGGAGA CACAGAGAAA ATTGTGTTCT TTGCGGGTGT CTCAAGACAG 
AAGGAAATCT ACATCATGGC TGCCAATTAC CTACAGTCCT TGGACTGGCG GAAGGAGCCA 
GAGATTATGA AGAATATCAT TAGCTTCTAC ACAAAAGGGC GGGCCTTGGA CCTCCTGGCT 
GGCTTCTATG ATGCCTGCGC CCAGGTGGAG ATTGACGAAT ACCAGAACTA TGACAAGGCC 
CATGGAGCAC TGACTGAGGC CTATAAGTGC CTGTCCAAAG CCAAGGCCAA GAACCCTCTG 
GACCAGGAGA CCAAGCTAGC CCAGCTACAG AGCAAGATGA CACTAGTGAA GAGGTTCATC 
CAGGCCCGAA GGACATACAC AGAGGACCCC AAAGAATCCG TCAGACAATG CGAGCTGCTC 
CTAGAAGAGC CAGACCTAGA CAGCACCATT CGTGTCGGGG ACATCTATGG CTTCCTGGTG 
GAGCACCATG TGCAGATGGA GGAATACCAG ATGGCCTATA AGTATCTTGA GGAGATGAGG 
AAAAGACTTC CTTCTGCCAA CATGTCATAC TATGTGGACC AGCGCACTGT GGACACTGTG 
CACCAGGGTC TGGGCCTCCC TCCATCTCGC ATCATGCCTG AGCGGGTCCG CCACAACAGC 
ATGGAGGACC ACAAGGAGGT GTACGAGGAG GTGATAGAAG AGGTGGACAA TGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246117.1
CDS	845..5104
Translation	MALYFDHRIKAPDASSSPSHITWHPTHPFLAVASVSPSSGGNVDIYLEQGEPVPDTHIERSFQATSLCWHPTRLILAIGWETGEVIMFNKQDKEQHTVPLPHTTDIAILSWSTSGSCLVSGDKLGVLLLWRLDQRGRVQGTPLLKHEYGKPLTHCIFRLPPPGEDLVQLAKAAVSGDEKALDMFNWRKSSFGSFLKTGSQEGLSFFVSLMDGTVHYVDEKGKTAQVASTDSSIQTLFYIEKREALVVVTQNLLLSLYVVNPEGEAEEVMKVKLSGKTGRRADITLIEGSLLVTAIGEPVLRFWDLERGENYILSLQEKFGFEKGENINSVCYCKAKGLLAAGTHKGRVAMWKKVPVSPSGRGAEGKDMWALQTPTELEGNITQIKWGSRKNLLAVSTSDSVSILSEQAMSSHFHQQVAAVQISPSLVNVSFLSTGSTHSLRTDMHISGVFATKDAVAVWNGKQVAIFEPSGSTLRNAGTFLCETSVLAMHEESIYTVEPNRLQVRTWQGTVKQLLLFSETEGSPCFLDVCGTFLVAGTDLAHFKSFDLSRREAKVHCSCKNLAQMIPDVGSITSLRCNANGNKISILLSKVDNSPDSKIYVYDVEMDTVNVFNFKTGHIGQIQTLPFNEPPTNEAHFFMNESLASYVPVNHFWDQSEPRLFVCEALQEAPGAQPQAIDKQPHMEEGTCHKPGEADKEDKMDSGHYHIPQMVAKKPLRDFVGLEDCDKPTRDAMLNFSFFVTIGDMDEAFKSIKLIKSETVWENMARMCVKTQRLDVAKVCLGNMGHARGARALREAEREPELEARVAMLAIQLGMLEEAEQLYKKCKRYDLLNKFYQASDQWQKAVEVAELHDRVHLRTTYYNYAKHLEASADCGLALSYYEKSDTHRFEVPRMLSEDLQSLELYINRMKDKTLWRWWAQYLESQAEMDTALRYYELAQDYFSLVRIHCFQGNIQKAAEIANETGDWAASYHLARQYESQDEVKQAVHFYTRAQAFNNAIRLCKENGLDDQLMNLALLSSPEDMIEAARYYEEKGEQMDRAVMLYHKAGHFSKALELAFTTQQFAALQLIAEDLDEKSDPALLARCSDFCIEHRQFEKAVELLLAAKKYHEALQLCLEQNMTITEEMAEKMTVSKDSKDLSEESRRELLEQIANCCMRQGNYHLATKKYTQAGNKLKAMRALLKSGDTEKIVFFAGVSRQKEIYIMAANYLQSLDWRKEPEIMKNIISFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLSKAKAKNPLDQETKLAQLQSKMTLVKRFIQARRTYTEDPKESVRQCELLLEEPDLDSTIRVGDIYGFLVEHHVQMEEYQMAYKYLEEMRKRLPSANMSYYVDQRTVDTVHQGLGLPPSRIMPERVRHNSMEDHKEVYEEVIEEVDNDP

Target ORF information:

RefSeq Version	XM_006246055.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 140 (Ift140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246055.3

ORF Insert Sequence:

ATGGCACTGT ATTTTGACCA CCGAATCAAA GCTCCAGATG CATCCAGCTC TCCCTCTCAC 
ATCACCTGGC ACCCCACCCA CCCATTCCTG GCAGTGGCAT CTGTAAGCCC ATCTTCTGGA 
GGCAACGTAG ACATCTACCT TGAACAAGGT GAGCCCGTGC CTGACACCCA CATTGAGAGG 
TCCTTCCAGG CCACTTCACT ATGCTGGCAC CCAACAAGGC TTATCCTGGC TATTGGCTGG 
GAAACTGGAG AAGTAATAAT GTTTAATAAG CAGGACAAGG AGCAGCACAC GGTGCCCCTG 
CCACATACGA CTGACATTGC CATCCTCAGT TGGAGTACCA GCGGGAGCTG CCTGGTATCT 
GGAGACAAGC TCGGGGTCCT GCTCTTGTGG AGATTGGACC AGCGAGGCCG AGTACAGGGG 
ACACCACTCC TGAAACACGA ATACGGGAAA CCGCTCACCC ATTGCATCTT CCGGCTCCCC 
CCTCCTGGGG AGGATCTGGT TCAGTTGGCA AAGGCAGCAG TAAGTGGGGA TGAGAAAGCC 
CTGGACATGT TTAACTGGAG AAAAAGCAGC TTTGGAAGTT TCCTGAAGAC GGGGTCTCAA 
GAGGGGCTGT CTTTCTTTGT CAGTCTCATG GATGGGACAG TGCACTATGT GGATGAAAAG 
GGCAAGACTG CTCAGGTGGC ATCCACGGAC AGCTCTATCC AGACCCTGTT CTACATTGAG 
AAGAGAGAGG CACTGGTTGT GGTCACACAG AACCTTCTCC TGTCTCTGTA TGTGGTGAAT 
CCTGAGGGGG AAGCTGAAGA AGTCATGAAG GTAAAGTTGA GTGGGAAGAC AGGCCGCCGG 
GCAGATATCA CTCTGATAGA GGGCAGTCTT CTTGTGACAG CTATTGGGGA ACCTGTGCTC 
AGGTTCTGGG ACTTAGAACG GGGAGAGAAT TACATACTGA GTCTACAGGA GAAGTTTGGC 
TTTGAAAAGG GAGAGAATAT AAACTCTGTG TGTTACTGTA AAGCCAAAGG TCTCCTGGCA 
GCTGGTACCC ATAAAGGACG AGTAGCCATG TGGAAGAAAG TGCCAGTGTC CCCAAGTGGC 
CGTGGGGCGG AGGGCAAGGA TATGTGGGCC CTTCAGACAC CTACTGAGCT TGAAGGAAAC 
ATCACACAGA TAAAGTGGGG CTCCAGGAAG AACCTGCTGG CGGTGAGCAC CTCTGACTCC 
GTGTCCATCC TCAGTGAGCA AGCCATGTCG TCACACTTCC ACCAGCAAGT GGCTGCCGTG 
CAGATCTCCC CGAGCCTAGT CAACGTGTCT TTCCTGTCCA CGGGGAGCAC GCACAGCCTG 
CGCACCGACA TGCACATCAG TGGAGTGTTT GCCACCAAGG ATGCTGTTGC TGTCTGGAAT 
GGAAAACAGG TGGCGATCTT TGAACCTTCA GGGTCCACCT TACGCAATGC AGGGACATTC 
CTGTGTGAAA CATCGGTGCT AGCCATGCAC GAGGAAAGCA TTTACACTGT AGAGCCAAAC 
CGACTCCAAG TCCGCACCTG GCAGGGCACT GTCAAACAAC TTCTGCTGTT CTCTGAGACC 
GAGGGGAGCC CCTGCTTCCT AGATGTCTGT GGGACTTTCC TGGTTGCAGG GACAGATTTA 
GCTCACTTTA AAAGTTTTGA TCTTTCCAGA AGGGAAGCAA AAGTGCACTG CAGCTGCAAG 
AACCTGGCCC AGATGATCCC CGACGTGGGG AGCATCACTT CACTGAGATG CAATGCCAAT 
GGGAACAAGA TCAGTATCCT CCTTAGCAAG GTTGACAACA GTCCTGATTC CAAAATCTAC 
GTCTATGACG TTGAAATGGA CACAGTGAAT GTCTTCAACT TCAAGACTGG ACACATTGGA 
CAGATACAGA CTCTGCCCTT TAATGAGCCT CCGACCAATG AGGCCCACTT CTTTATGAAT 
GAGAGCCTAG CAAGTTACGT TCCTGTGAAC CATTTCTGGG ATCAGAGTGA ACCCAGGCTC 
TTTGTGTGCG AGGCCTTGCA GGAGGCACCT GGAGCCCAGC CACAGGCTAT AGACAAGCAG 
CCCCACATGG AGGAAGGCAC GTGCCACAAG CCTGGAGAAG CAGACAAAGA AGACAAGATG 
GATTCTGGGC ACTACCACAT CCCTCAGATG GTAGCCAAGA AGCCCCTACG AGACTTTGTG 
GGGCTGGAGG ACTGTGACAA GCCTACTCGG GATGCCATGC TCAACTTCAG CTTCTTTGTC 
ACCATTGGAG ACATGGATGA AGCTTTCAAA TCTATCAAAC TCATCAAGAG TGAGACCGTC 
TGGGAGAACA TGGCACGCAT GTGTGTGAAG ACTCAGAGGC TGGATGTTGC CAAGGTGTGC 
CTAGGGAACA TGGGCCATGC CCGGGGGGCC CGAGCACTGC GGGAAGCTGA GCGGGAACCA 
GAACTAGAAG CCAGAGTGGC CATGTTGGCC ATACAGCTGG GCATGCTGGA GGAGGCTGAA 
CAACTGTATA AGAAGTGCAA ACGCTACGAC CTCCTAAACA AGTTCTACCA GGCCTCAGAC 
CAGTGGCAGA AAGCTGTGGA GGTAGCAGAG CTCCATGACC GAGTCCACCT GCGCACCACC 
TACTACAACT ATGCCAAGCA CCTGGAGGCC AGCGCCGACT GCGGCCTGGC CCTCAGCTAC 
TATGAGAAGT CAGACACACA CCGTTTTGAG GTACCTAGGA TGCTCTCAGA AGACCTGCAG 
TCACTGGAGC TGTACATCAA TAGGATGAAG GACAAGACCC TGTGGAGATG GTGGGCCCAG 
TACCTGGAGA GCCAGGCAGA GATGGACACC GCGTTGCGCT ACTACGAGCT GGCCCAGGAC 
TACTTCTCCC TGGTCCGTAT TCACTGCTTC CAGGGCAACA TTCAGAAGGC TGCTGAAATA 
GCCAACGAGA CTGGAGACTG GGCTGCATCC TACCACCTTG CCCGGCAGTA CGAGAGCCAG 
GATGAGGTAA AACAGGCAGT GCACTTCTAC ACACGGGCAC AGGCTTTCAA CAACGCCATC 
CGCCTGTGCA AGGAGAATGG CTTAGATGAC CAACTCATGA ACTTGGCCCT CTTGAGCTCC 
CCCGAGGACA TGATTGAGGC AGCCCGGTAT TACGAGGAGA AGGGCGAGCA GATGGACAGG 
GCAGTCATGC TGTATCACAA GGCTGGACAC TTCTCCAAGG CCCTGGAGCT GGCCTTCACC 
ACCCAGCAGT TTGCAGCCCT ACAACTCATA GCAGAGGACC TGGATGAGAA GTCAGATCCT 
GCTCTCCTGG CCCGCTGCTC AGACTTCTGC ATTGAGCACA GACAGTTTGA AAAGGCCGTG 
GAGCTTCTGC TGGCTGCCAA GAAGTACCAC GAGGCTCTGC AGCTGTGCCT AGAGCAGAAC 
ATGACCATCA CTGAGGAGAT GGCCGAGAAG ATGACTGTGT CCAAGGACTC GAAGGACCTG 
TCCGAGGAGT CACGGCGTGA GCTGCTAGAA CAGATTGCAA ACTGCTGTAT GCGCCAGGGC 
AACTATCACC TGGCTACCAA GAAGTACACA CAGGCTGGGA ACAAGCTGAA GGCCATGAGA 
GCCCTGCTCA AATCTGGAGA CACAGAGAAA ATTGTGTTCT TTGCGGGTGT CTCAAGACAG 
AAGGAAATCT ACATCATGGC TGCCAATTAC CTACAGTCCT TGGACTGGCG GAAGGAGCCA 
GAGATTATGA AGAATATCAT TAGCTTCTAC ACAAAAGGGC GGGCCTTGGA CCTCCTGGCT 
GGCTTCTATG ATGCCTGCGC CCAGGTGGAG ATTGACGAAT ACCAGAACTA TGACAAGGCC 
CATGGAGCAC TGACTGAGGC CTATAAGTGC CTGTCCAAAG CCAAGGCCAA GAACCCTCTG 
GACCAGGAGA CCAAGCTAGC CCAGCTACAG AGCAAGATGA CACTAGTGAA GAGGTTCATC 
CAGGCCCGAA GGACATACAC AGAGGACCCC AAAGAATCCG TCAGACAATG CGAGCTGCTC 
CTAGAAGAGC CAGACCTAGA CAGCACCATT CGTGTCGGGG ACATCTATGG CTTCCTGGTG 
GAGCACCATG TGCAGATGGA GGAATACCAG ATGGCCTATA AGTATCTTGA GGAGATGAGG 
AAAAGACTTC CTTCTGCCAA CATGTCATAC TATGTGGACC AGCGCACTGT GGACACTGTG 
CACCAGGGTC TGGGCCTCCC TCCATCTCGC ATCATGCCTG AGCGGGTCCG CCACAACAGC 
ATGGAGGACC ACAAGGAGGT GTACGAGGAG GTGATAGAAG AGGTGGACAA TGACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Ift140 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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