Mkrn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mkrn2
Entrez Gene ID	67027
Full Name	makorin, ring finger protein, 2
Synonyms	2610002L04Rik,C81377
General protein information	Preferred Names makorin, ring finger protein, 2 Names probable E3 ubiquitin-protein ligase makorin-2 Makorin RING zinc-finger protein 2 RING-type E3 ubiquitin transferase makorin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_023290.3	NP_075779.2	probable E3 ubiquitin-protein ligase makorin-2
XM_011241431.2	XP_011239733.1	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
NM_023290.2	NP_075779.2	probable E3 ubiquitin-protein ligase makorin-2
XM_011241431.1	XP_011239733.1	probable E3 ubiquitin-protein ligase makorin-2 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MKRN2	XP_001086204.2
Canis lupus familiaris (dog)	MKRN2	XP_005632195.1
Arabidopsis thaliana (thale cress)	AT3G08505	NP_974255.1
Oryza sativa (rice)	Os06g0318700	NP_001057505.1
Pan troglodytes (chimpanzee)	MKRN2	XP_516287.3
Gallus gallus (chicken)	MKRN2	NP_989619.1
Homo sapiens (human)	MKRN2	NP_054879.3
Bos taurus (cattle)	MKRN2	NP_001039454.1
Mus musculus (house mouse)	Mkrn2	NP_075779.2
Rattus norvegicus (Norway rat)	Mkrn2	NP_001008315.1
Danio rerio (zebrafish)	mkrn2	NP_694511.1

Gene Ontology
Bibliography

Related articles in PubMed

MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
Shin C, Ito Y, Ichikawa S, Tokunaga M, Sakata-Sogawa K, Tanaka T
Scientific reports746097(2017 04)

Deficiency of Mkrn2 causes abnormal spermiogenesis and spermiation, and impairs male fertility.
Qian X, Wang L, Zheng B, Shi ZM, Ge X, Jiang CF, Qian YC, Li DM, Li W, Liu X, Yin Y, Zheng JT, Shen H, Wang M, Guo XJ, He J, Lin M, Liu LZ, Sha JH, Jiang BH
Scientific reports639318(2016 12)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene.
Gray TA, Azama K, Whitmore K, Min A, Abe S, Nicholls RD
Genomics77(3)119-26(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mkrn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mkrn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu70059	XM_011241431.2 Latest version!	Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu01270	NM_023290.3 Latest version!	Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OMu01270	NM_023290.2	Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OMu70059	XM_011241431.1	Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mkrn2 ( NM_023290.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075779.2 Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.

Price & Availability↑

CloneID	OMu01270
Clone ID Related Accession (Same CDS sequence)	NM_023290.3 , NM_023290.2
Accession Version	NM_023290.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509206400000
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase makorin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077926.1. On Feb 25, 2006 this sequence version replaced NM_023290.1. ##Evidence-Data-START## Transcript exon combination :: AK077926.1, SRR1660813.238293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACCA AGCAGGTCAC CTGCAGGTAT TTTATGCATG GCGTATGTCG CGAAGGCAGC 
CAGTGCCTGT TCTCACATGA CTTGGCGAAC AGCAAGCCGT CCACCATCTG CAAATACTAC 
CAGAAGGGCT ACTGCGCCTA TGGTGCACGC TGCAGATATG ATCATACAAA GCCCCCAGCT 
GCAGCAGGTG GAGCTGTGGG TCCTGCACCC AACCCTTCGC CCTCCTCAGG CCTGCATAGT 
CCTCACCCTT CTCCTGACAT TGCCACATCT GTTATGAGGA CACATTCGAA TGAACCTGGA 
AAACGGGAGA AGAAGACGCT GGTGCTCAGA GACCGCAATC TCACTGGTCT GGCTGAAGAT 
AAGACTCCTC CAAGCAAAGT GAATAATCCA GGTGGCTGCA GTGACCCTCA GACCAGCCCA 
GAGATGAAGC CCCATTCCTA CCTAGATGCC ATCAGGACTG GCCTGGATGA CTTGGAGGCC 
AGCAGCTCCT ACAGCAATGA GCCGCAGTTG TGCCCCTATG CTGCCGCTGG GGAGTGTCGC 
TTTGGAGATG CCTGTGTCTA CCTGCATGGG GACATGTGTG AGATCTGCAG GCTTCAAGTC 
CTCCACCCCT TTGACCCAGA ACAAAGGAAA GCTCACGAGA AGATGTGTAT GTCGACATTT 
GAACATGAGA TGGAAAAGGC GTTTGCCTTC CAGGCGAGCC AGGACAAAGT GTGCAGCATC 
TGCATGGAGG TGATCCTGGA GAAAGCATCT GCTTCTGAAA GGAGGTTTGG CATTCTCTCC 
AACTGCAGCC ACACCTACTG CCTGTCCTGC ATCCGTCAAT GGAGATGTGC CAAGCAGTTT 
GAAAACCCAA TCATAAAGTC TTGTCCAGAG TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTAGAAGA TCAGAATAAA AAGAATGAAT TAATTGAAGC TTTCAAACAG 
GGAATGGGGA AGAAGGCATG CAAATACTTT GAACAAGGCA AGGGGACCTG CCCCTTTGGA 
AGCAAGTGCC TTTACCGTCA TGCTTACCCT GATGGGCGGC TGGCAGAGCC AGAGAAACCT 
CGGAAGCAGC TAAGCTCTGA AGGCACTGTG AGGTTCTTTA ACTCAGTTCG GCTTTGGGAT 
TTTATCGAGA ACCGAGAAAC CCGGCAAGTC CCCAGCACTG ACGATGTGGA TGTGACAGAG 
CTAGGAGACC TCTTCATGCA CCTTTCTGGA GTAGAATCAT CAGAACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075779.2
CDS	52..1302
Translation	MSTKQVTCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGARCRYDHTKPPAAAGGAVGPAPNPSPSSGLHSPHPSPDIATSVMRTHSNEPGKREKKTLVLRDRNLTGLAEDKTPPSKVNNPGGCSDPQTSPEMKPHSYLDAIRTGLDDLEASSSYSNEPQLCPYAAAGECRFGDACVYLHGDMCEICRLQVLHPFDPEQRKAHEKMCMSTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSEGTVRFFNSVRLWDFIENRETRQVPSTDDVDVTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	NM_023290.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023290.2

ORF Insert Sequence:

ATGAGCACCA AGCAGGTCAC CTGCAGGTAT TTTATGCATG GCGTATGTCG CGAAGGCAGC 
CAGTGCCTGT TCTCACATGA CTTGGCGAAC AGCAAGCCGT CCACCATCTG CAAATACTAC 
CAGAAGGGCT ACTGCGCCTA TGGTGCACGC TGCAGATATG ATCATACAAA GCCCCCAGCT 
GCAGCAGGTG GAGCTGTGGG TCCTGCACCC AACCCTTCGC CCTCCTCAGG CCTGCATAGT 
CCTCACCCTT CTCCTGACAT TGCCACATCT GTTATGAGGA CACATTCGAA TGAACCTGGA 
AAACGGGAGA AGAAGACGCT GGTGCTCAGA GACCGCAATC TCACTGGTCT GGCTGAAGAT 
AAGACTCCTC CAAGCAAAGT GAATAATCCA GGTGGCTGCA GTGACCCTCA GACCAGCCCA 
GAGATGAAGC CCCATTCCTA CCTAGATGCC ATCAGGACTG GCCTGGATGA CTTGGAGGCC 
AGCAGCTCCT ACAGCAATGA GCCGCAGTTG TGCCCCTATG CTGCCGCTGG GGAGTGTCGC 
TTTGGAGATG CCTGTGTCTA CCTGCATGGG GACATGTGTG AGATCTGCAG GCTTCAAGTC 
CTCCACCCCT TTGACCCAGA ACAAAGGAAA GCTCACGAGA AGATGTGTAT GTCGACATTT 
GAACATGAGA TGGAAAAGGC GTTTGCCTTC CAGGCGAGCC AGGACAAAGT GTGCAGCATC 
TGCATGGAGG TGATCCTGGA GAAAGCATCT GCTTCTGAAA GGAGGTTTGG CATTCTCTCC 
AACTGCAGCC ACACCTACTG CCTGTCCTGC ATCCGTCAAT GGAGATGTGC CAAGCAGTTT 
GAAAACCCAA TCATAAAGTC TTGTCCAGAG TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTAGAAGA TCAGAATAAA AAGAATGAAT TAATTGAAGC TTTCAAACAG 
GGAATGGGGA AGAAGGCATG CAAATACTTT GAACAAGGCA AGGGGACCTG CCCCTTTGGA 
AGCAAGTGCC TTTACCGTCA TGCTTACCCT GATGGGCGGC TGGCAGAGCC AGAGAAACCT 
CGGAAGCAGC TAAGCTCTGA AGGCACTGTG AGGTTCTTTA ACTCAGTTCG GCTTTGGGAT 
TTTATCGAGA ACCGAGAAAC CCGGCAAGTC CCCAGCACTG ACGATGTGGA TGTGACAGAG 
CTAGGAGACC TCTTCATGCA CCTTTCTGGA GTAGAATCAT CAGAACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkrn2 ( XM_011241431.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239733.1 Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70059
Clone ID Related Accession (Same CDS sequence)	XM_011241431.2 , XM_011241431.1
Accession Version	XM_011241431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AAAACGGGAG AAGAAGACGC TGGTGCTCAG AGACCGCAAT GTGTATGTCG 
ACATTTGAAC ATGAGATGGA AAAGGCGTTT GCCTTCCAGG CGAGCCAGGA CAAAGTGTGC 
AGCATCTGCA TGGAGGTGAT CCTGGAGAAA GCATCTGCTT CTGAAAGGAG GTTTGGCATT 
CTCTCCAACT GCAGCCACAC CTACTGCCTG TCCTGCATCC GTCAATGGAG ATGTGCCAAG 
CAGTTTGAAA ACCCAATCAT AAAGTCTTGT CCAGAGTGCC GTGTGATATC AGAGTTTGTA 
ATTCCAAGTG TGTATTGGGT AGAAGATCAG AATAAAAAGA ATGAATTAAT TGAAGCTTTC 
AAACAGGGAA TGGGGAAGAA GGCATGCAAA TACTTTGAAC AAGGCAAGGG GACCTGCCCC 
TTTGGAAGCA AGTGCCTTTA CCGTCATGCT TACCCTGATG GGCGGCTGGC AGAGCCAGAG 
AAACCTCGGA AGCAGCTAAG CTCTGAAGGC ACTGTGAGGT TCTTTAACTC AGTTCGGCTT 
TGGGATTTTA TCGAGAACCG AGAAACCCGG CAAGTCCCCA GCACTGACGA TGTGGATGTG 
ACAGAGCTAG GAGACCTCTT CATGCACCTT TCTGGAGTAG AATCATCAGA ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239733.1
CDS	395..1051
Translation	MNLENGRRRRWCSETAMCMSTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSEGTVRFFNSVRLWDFIENRETRQVPSTDDVDVTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	XM_011241431.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241431.2

ORF Insert Sequence:

ATGAACCTGG AAAACGGGAG AAGAAGACGC TGGTGCTCAG AGACCGCAAT GTGTATGTCG 
ACATTTGAAC ATGAGATGGA AAAGGCGTTT GCCTTCCAGG CGAGCCAGGA CAAAGTGTGC 
AGCATCTGCA TGGAGGTGAT CCTGGAGAAA GCATCTGCTT CTGAAAGGAG GTTTGGCATT 
CTCTCCAACT GCAGCCACAC CTACTGCCTG TCCTGCATCC GTCAATGGAG ATGTGCCAAG 
CAGTTTGAAA ACCCAATCAT AAAGTCTTGT CCAGAGTGCC GTGTGATATC AGAGTTTGTA 
ATTCCAAGTG TGTATTGGGT AGAAGATCAG AATAAAAAGA ATGAATTAAT TGAAGCTTTC 
AAACAGGGAA TGGGGAAGAA GGCATGCAAA TACTTTGAAC AAGGCAAGGG GACCTGCCCC 
TTTGGAAGCA AGTGCCTTTA CCGTCATGCT TACCCTGATG GGCGGCTGGC AGAGCCAGAG 
AAACCTCGGA AGCAGCTAAG CTCTGAAGGC ACTGTGAGGT TCTTTAACTC AGTTCGGCTT 
TGGGATTTTA TCGAGAACCG AGAAACCCGG CAAGTCCCCA GCACTGACGA TGTGGATGTG 
ACAGAGCTAG GAGACCTCTT CATGCACCTT TCTGGAGTAG AATCATCAGA ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkrn2 ( XM_011241431.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239733.1 Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70059
Clone ID Related Accession (Same CDS sequence)	XM_011241431.2 , XM_011241431.1
Accession Version	XM_011241431.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AAAACGGGAG AAGAAGACGC TGGTGCTCAG AGACCGCAAT GTGTATGTCG 
ACATTTGAAC ATGAGATGGA AAAGGCGTTT GCCTTCCAGG CGAGCCAGGA CAAAGTGTGC 
AGCATCTGCA TGGAGGTGAT CCTGGAGAAA GCATCTGCTT CTGAAAGGAG GTTTGGCATT 
CTCTCCAACT GCAGCCACAC CTACTGCCTG TCCTGCATCC GTCAATGGAG ATGTGCCAAG 
CAGTTTGAAA ACCCAATCAT AAAGTCTTGT CCAGAGTGCC GTGTGATATC AGAGTTTGTA 
ATTCCAAGTG TGTATTGGGT AGAAGATCAG AATAAAAAGA ATGAATTAAT TGAAGCTTTC 
AAACAGGGAA TGGGGAAGAA GGCATGCAAA TACTTTGAAC AAGGCAAGGG GACCTGCCCC 
TTTGGAAGCA AGTGCCTTTA CCGTCATGCT TACCCTGATG GGCGGCTGGC AGAGCCAGAG 
AAACCTCGGA AGCAGCTAAG CTCTGAAGGC ACTGTGAGGT TCTTTAACTC AGTTCGGCTT 
TGGGATTTTA TCGAGAACCG AGAAACCCGG CAAGTCCCCA GCACTGACGA TGTGGATGTG 
ACAGAGCTAG GAGACCTCTT CATGCACCTT TCTGGAGTAG AATCATCAGA ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239733.1
CDS	317..973
Translation	MNLENGRRRRWCSETAMCMSTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSEGTVRFFNSVRLWDFIENRETRQVPSTDDVDVTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	XM_011241431.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus makorin, ring finger protein, 2 (Mkrn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241431.1

ORF Insert Sequence:

ATGAACCTGG AAAACGGGAG AAGAAGACGC TGGTGCTCAG AGACCGCAAT GTGTATGTCG 
ACATTTGAAC ATGAGATGGA AAAGGCGTTT GCCTTCCAGG CGAGCCAGGA CAAAGTGTGC 
AGCATCTGCA TGGAGGTGAT CCTGGAGAAA GCATCTGCTT CTGAAAGGAG GTTTGGCATT 
CTCTCCAACT GCAGCCACAC CTACTGCCTG TCCTGCATCC GTCAATGGAG ATGTGCCAAG 
CAGTTTGAAA ACCCAATCAT AAAGTCTTGT CCAGAGTGCC GTGTGATATC AGAGTTTGTA 
ATTCCAAGTG TGTATTGGGT AGAAGATCAG AATAAAAAGA ATGAATTAAT TGAAGCTTTC 
AAACAGGGAA TGGGGAAGAA GGCATGCAAA TACTTTGAAC AAGGCAAGGG GACCTGCCCC 
TTTGGAAGCA AGTGCCTTTA CCGTCATGCT TACCCTGATG GGCGGCTGGC AGAGCCAGAG 
AAACCTCGGA AGCAGCTAAG CTCTGAAGGC ACTGTGAGGT TCTTTAACTC AGTTCGGCTT 
TGGGATTTTA TCGAGAACCG AGAAACCCGG CAAGTCCCCA GCACTGACGA TGTGGATGTG 
ACAGAGCTAG GAGACCTCTT CATGCACCTT TCTGGAGTAG AATCATCAGA ACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkrn2 ( NM_023290.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075779.2 Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.

Price & Availability↑

CloneID	OMu01270
Clone ID Related Accession (Same CDS sequence)	NM_023290.3 , NM_023290.2
Accession Version	NM_023290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase makorin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160032.7. On Aug 14, 2018 this sequence version replaced NM_023290.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK077926.1, AK011295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACCA AGCAGGTCAC CTGCAGGTAT TTTATGCATG GCGTATGTCG CGAAGGCAGC 
CAGTGCCTGT TCTCACATGA CTTGGCGAAC AGCAAGCCGT CCACCATCTG CAAATACTAC 
CAGAAGGGCT ACTGCGCCTA TGGTGCACGC TGCAGATATG ATCATACAAA GCCCCCAGCT 
GCAGCAGGTG GAGCTGTGGG TCCTGCACCC AACCCTTCGC CCTCCTCAGG CCTGCATAGT 
CCTCACCCTT CTCCTGACAT TGCCACATCT GTTATGAGGA CACATTCGAA TGAACCTGGA 
AAACGGGAGA AGAAGACGCT GGTGCTCAGA GACCGCAATC TCACTGGTCT GGCTGAAGAT 
AAGACTCCTC CAAGCAAAGT GAATAATCCA GGTGGCTGCA GTGACCCTCA GACCAGCCCA 
GAGATGAAGC CCCATTCCTA CCTAGATGCC ATCAGGACTG GCCTGGATGA CTTGGAGGCC 
AGCAGCTCCT ACAGCAATGA GCCGCAGTTG TGCCCCTATG CTGCCGCTGG GGAGTGTCGC 
TTTGGAGATG CCTGTGTCTA CCTGCATGGG GACATGTGTG AGATCTGCAG GCTTCAAGTC 
CTCCACCCCT TTGACCCAGA ACAAAGGAAA GCTCACGAGA AGATGTGTAT GTCGACATTT 
GAACATGAGA TGGAAAAGGC GTTTGCCTTC CAGGCGAGCC AGGACAAAGT GTGCAGCATC 
TGCATGGAGG TGATCCTGGA GAAAGCATCT GCTTCTGAAA GGAGGTTTGG CATTCTCTCC 
AACTGCAGCC ACACCTACTG CCTGTCCTGC ATCCGTCAAT GGAGATGTGC CAAGCAGTTT 
GAAAACCCAA TCATAAAGTC TTGTCCAGAG TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTAGAAGA TCAGAATAAA AAGAATGAAT TAATTGAAGC TTTCAAACAG 
GGAATGGGGA AGAAGGCATG CAAATACTTT GAACAAGGCA AGGGGACCTG CCCCTTTGGA 
AGCAAGTGCC TTTACCGTCA TGCTTACCCT GATGGGCGGC TGGCAGAGCC AGAGAAACCT 
CGGAAGCAGC TAAGCTCTGA AGGCACTGTG AGGTTCTTTA ACTCAGTTCG GCTTTGGGAT 
TTTATCGAGA ACCGAGAAAC CCGGCAAGTC CCCAGCACTG ACGATGTGGA TGTGACAGAG 
CTAGGAGACC TCTTCATGCA CCTTTCTGGA GTAGAATCAT CAGAACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075779.2
CDS	51..1301
Translation	MSTKQVTCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGARCRYDHTKPPAAAGGAVGPAPNPSPSSGLHSPHPSPDIATSVMRTHSNEPGKREKKTLVLRDRNLTGLAEDKTPPSKVNNPGGCSDPQTSPEMKPHSYLDAIRTGLDDLEASSSYSNEPQLCPYAAAGECRFGDACVYLHGDMCEICRLQVLHPFDPEQRKAHEKMCMSTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSEGTVRFFNSVRLWDFIENRETRQVPSTDDVDVTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	NM_023290.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023290.3

ORF Insert Sequence:

ATGAGCACCA AGCAGGTCAC CTGCAGGTAT TTTATGCATG GCGTATGTCG CGAAGGCAGC 
CAGTGCCTGT TCTCACATGA CTTGGCGAAC AGCAAGCCGT CCACCATCTG CAAATACTAC 
CAGAAGGGCT ACTGCGCCTA TGGTGCACGC TGCAGATATG ATCATACAAA GCCCCCAGCT 
GCAGCAGGTG GAGCTGTGGG TCCTGCACCC AACCCTTCGC CCTCCTCAGG CCTGCATAGT 
CCTCACCCTT CTCCTGACAT TGCCACATCT GTTATGAGGA CACATTCGAA TGAACCTGGA 
AAACGGGAGA AGAAGACGCT GGTGCTCAGA GACCGCAATC TCACTGGTCT GGCTGAAGAT 
AAGACTCCTC CAAGCAAAGT GAATAATCCA GGTGGCTGCA GTGACCCTCA GACCAGCCCA 
GAGATGAAGC CCCATTCCTA CCTAGATGCC ATCAGGACTG GCCTGGATGA CTTGGAGGCC 
AGCAGCTCCT ACAGCAATGA GCCGCAGTTG TGCCCCTATG CTGCCGCTGG GGAGTGTCGC 
TTTGGAGATG CCTGTGTCTA CCTGCATGGG GACATGTGTG AGATCTGCAG GCTTCAAGTC 
CTCCACCCCT TTGACCCAGA ACAAAGGAAA GCTCACGAGA AGATGTGTAT GTCGACATTT 
GAACATGAGA TGGAAAAGGC GTTTGCCTTC CAGGCGAGCC AGGACAAAGT GTGCAGCATC 
TGCATGGAGG TGATCCTGGA GAAAGCATCT GCTTCTGAAA GGAGGTTTGG CATTCTCTCC 
AACTGCAGCC ACACCTACTG CCTGTCCTGC ATCCGTCAAT GGAGATGTGC CAAGCAGTTT 
GAAAACCCAA TCATAAAGTC TTGTCCAGAG TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTAGAAGA TCAGAATAAA AAGAATGAAT TAATTGAAGC TTTCAAACAG 
GGAATGGGGA AGAAGGCATG CAAATACTTT GAACAAGGCA AGGGGACCTG CCCCTTTGGA 
AGCAAGTGCC TTTACCGTCA TGCTTACCCT GATGGGCGGC TGGCAGAGCC AGAGAAACCT 
CGGAAGCAGC TAAGCTCTGA AGGCACTGTG AGGTTCTTTA ACTCAGTTCG GCTTTGGGAT 
TTTATCGAGA ACCGAGAAAC CCGGCAAGTC CCCAGCACTG ACGATGTGGA TGTGACAGAG 
CTAGGAGACC TCTTCATGCA CCTTTCTGGA GTAGAATCAT CAGAACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
Scientific reports746097(2017 04)
Shin C,Ito Y,Ichikawa S,Tokunaga M,Sakata-Sogawa K,Tanaka T

Deficiency of Mkrn2 causes abnormal spermiogenesis and spermiation, and impairs male fertility.
Scientific reports639318(2016 12)
Qian X,Wang L,Zheng B,Shi ZM,Ge X,Jiang CF,Qian YC,Li DM,Li W,Liu X,Yin Y,Zheng JT,Shen H,Wang M,Guo XJ,He J,Lin M,Liu LZ,Sha JH,Jiang BH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene.
Genomics77(3)119-26(2001 Oct)
Gray TA,Azama K,Whitmore K,Min A,Abe S,Nicholls RD

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Mkrn2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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