MKRN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MKRN2
Entrez Gene ID	23609
Full Name	makorin ring finger protein 2
Synonyms	HSPC070,RNF62
General protein information	Preferred Names makorin ring finger protein 2 Names probable E3 ubiquitin-protein ligase makorin-2 RING finger protein 62 RING-type E3 ubiquitin transferase makorin-2 makorin RING zinc-finger protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.2
Summary	This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Disorder MIM:	608426

mRNA and Protein(s)

mRNA	Protein	Name
NM_014160.4	NP_054879.3	probable E3 ubiquitin-protein ligase makorin-2 isoform 1
NM_014160.5	NP_054879.3	probable E3 ubiquitin-protein ligase makorin-2 isoform 1
NM_001271707.1	NP_001258636.1	probable E3 ubiquitin-protein ligase makorin-2 isoform 2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MKRN2	XP_001086204.2
Canis lupus familiaris (dog)	MKRN2	XP_005632195.1
Arabidopsis thaliana (thale cress)	AT3G08505	NP_974255.1
Oryza sativa (rice)	Os06g0318700	NP_001057505.1
Pan troglodytes (chimpanzee)	MKRN2	XP_516287.3
Gallus gallus (chicken)	MKRN2	NP_989619.1
Homo sapiens (human)	MKRN2	NP_054879.3
Bos taurus (cattle)	MKRN2	NP_001039454.1
Mus musculus (house mouse)	Mkrn2	NP_075779.2
Rattus norvegicus (Norway rat)	Mkrn2	NP_001008315.1
Danio rerio (zebrafish)	mkrn2	NP_694511.1

Gene Ontology
Bibliography

Related articles in PubMed

MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway.
Jiang J, Xu Y, Ren H, Wudu M, Wang Q, Song X, Su H, Jiang X, Jiang L, Qiu X
Journal of experimental & clinical cancer research : CR37(1)189(2018 Aug)

MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
Shin C, Ito Y, Ichikawa S, Tokunaga M, Sakata-Sogawa K, Tanaka T
Scientific reports746097(2017 04)

Ubiquitous expression of MAKORIN-2 in normal and malignant hematopoietic cells and its growth promoting activity.
Lee KY, Chan KY, Tsang KS, Chen YC, Kung HF, Ng PC, Li CK, Leung KT, Li K
PloS one9(3)e92706(2014)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
Böhne A, Darras A, D'Cotta H, Baroiller JF, Galiana-Arnoux D, Volff JN
BMC genomics11721(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MKRN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MKRN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14185	NM_001271707.1 Latest version!	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu14084	NM_014160.5 Latest version!	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu14084	NM_014160.4	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MKRN2 ( NM_014160.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054879.3 Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14084
Clone ID Related Accession (Same CDS sequence)	NM_014160.4 , NM_014160.5
Accession Version	NM_014160.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR157266.1, BC015715.1 and AK298463.1. On Nov 9, 2012 this sequence version replaced NM_014160.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC015715.1, SRR1803616.245080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACCA AGCAGATCAC TTGCAGGTAT TTTATGCATG GTGTGTGTCG GGAAGGAAGT 
CAGTGCCTAT TCTCACATGA CTTGGCAAAC AGCAAACCGT CCACCATCTG CAAGTACTAC 
CAGAAGGGCT ACTGTGCCTA TGGAACTCGG TGCAGATATG ACCACACGAG GCCCTCTGCT 
GCAGCTGGAG GTGCTGTGGG CACCATGGCC CACAGTGTGC CCTCCCCAGC TTTCCACAGT 
CCTCACCCTC CTTCCGAGGT CACTGCATCC ATTGTGAAAA CTAACTCACA TGAACCCGGA 
AAGCGTGAAA AGAGAACATT GGTTCTTAGA GACCGAAATC TCTCTGGCAT GGCTGAAAGG 
AAGACCCAGC CGAGCATGGT GAGTAATCCA GGCAGCTGCA GCGACCCCCA GCCCAGCCCC 
GAGATGAAGC CGCATTCCTA CCTGGATGCC ATCAGGAGTG GCCTTGATGA CGTGGAGGCC 
AGCAGCTCCT ACAGCAACGA GCAGCAGCTG TGCCCCTACG CAGCTGCTGG GGAGTGCCGG 
TTTGGGGATG CCTGTGTCTA CCTGCACGGG GAGGTGTGTG AAATCTGTAG GCTGCAAGTC 
TTGCACCCAT TCGACCCAGA GCAGAGGAAG GCTCATGAAA AGATCTGCAT GTTGACGTTC 
GAACACGAGA TGGAAAAGGC CTTTGCCTTC CAGGCAAGCC AGGACAAAGT GTGCAGTATC 
TGCATGGAAG TGATCCTGGA GAAGGCCTCT GCTTCTGAGA GGAGATTTGG GATTCTCTCC 
AATTGCAATC ACACGTACTG TTTGTCCTGC ATCCGGCAGT GGCGGTGTGC CAAACAGTTT 
GAAAACCCAA TCATTAAGTC TTGTCCAGAA TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTGGAAGA TCAGAATAAA AAGAACGAGT TGATTGAAGC TTTCAAACAG 
GGGATGGGGA AAAAAGCCTG TAAATACTTT GAGCAAGGCA AGGGGACCTG CCCATTTGGA 
AGCAAATGTC TTTATCGCCA TGCTTACCCC GATGGGCGGC TAGCAGAGCC TGAGAAACCT 
CGGAAACAGC TCAGTTCTCA AGGCACTGTG AGGTTCTTTA ATTCAGTGCG GCTCTGGGAT 
TTCATCGAGA ACCGAGAAAG CCGGCATGTC CCCAACAATG AAGATGTCGA CATGACAGAG 
CTCGGGGACC TCTTCATGCA CCTTTCTGGA GTGGAATCAT CAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054879.3
CDS	138..1388
Translation	MSTKQITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDHTRPSAAAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	NM_014160.4
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014160.4

ORF Insert Sequence:

ATGAGCACCA AGCAGATCAC TTGCAGGTAT TTTATGCATG GTGTGTGTCG GGAAGGAAGT 
CAGTGCCTAT TCTCACATGA CTTGGCAAAC AGCAAACCGT CCACCATCTG CAAGTACTAC 
CAGAAGGGCT ACTGTGCCTA TGGAACTCGG TGCAGATATG ACCACACGAG GCCCTCTGCT 
GCAGCTGGAG GTGCTGTGGG CACCATGGCC CACAGTGTGC CCTCCCCAGC TTTCCACAGT 
CCTCACCCTC CTTCCGAGGT CACTGCATCC ATTGTGAAAA CTAACTCACA TGAACCCGGA 
AAGCGTGAAA AGAGAACATT GGTTCTTAGA GACCGAAATC TCTCTGGCAT GGCTGAAAGG 
AAGACCCAGC CGAGCATGGT GAGTAATCCA GGCAGCTGCA GCGACCCCCA GCCCAGCCCC 
GAGATGAAGC CGCATTCCTA CCTGGATGCC ATCAGGAGTG GCCTTGATGA CGTGGAGGCC 
AGCAGCTCCT ACAGCAACGA GCAGCAGCTG TGCCCCTACG CAGCTGCTGG GGAGTGCCGG 
TTTGGGGATG CCTGTGTCTA CCTGCACGGG GAGGTGTGTG AAATCTGTAG GCTGCAAGTC 
TTGCACCCAT TCGACCCAGA GCAGAGGAAG GCTCATGAAA AGATCTGCAT GTTGACGTTC 
GAACACGAGA TGGAAAAGGC CTTTGCCTTC CAGGCAAGCC AGGACAAAGT GTGCAGTATC 
TGCATGGAAG TGATCCTGGA GAAGGCCTCT GCTTCTGAGA GGAGATTTGG GATTCTCTCC 
AATTGCAATC ACACGTACTG TTTGTCCTGC ATCCGGCAGT GGCGGTGTGC CAAACAGTTT 
GAAAACCCAA TCATTAAGTC TTGTCCAGAA TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTGGAAGA TCAGAATAAA AAGAACGAGT TGATTGAAGC TTTCAAACAG 
GGGATGGGGA AAAAAGCCTG TAAATACTTT GAGCAAGGCA AGGGGACCTG CCCATTTGGA 
AGCAAATGTC TTTATCGCCA TGCTTACCCC GATGGGCGGC TAGCAGAGCC TGAGAAACCT 
CGGAAACAGC TCAGTTCTCA AGGCACTGTG AGGTTCTTTA ATTCAGTGCG GCTCTGGGAT 
TTCATCGAGA ACCGAGAAAG CCGGCATGTC CCCAACAATG AAGATGTCGA CATGACAGAG 
CTCGGGGACC TCTTCATGCA CCTTTCTGGA GTGGAATCAT CAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN2 ( NM_001271707.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258636.1 Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14185
Clone ID Related Accession (Same CDS sequence)	NM_001271707.1
Accession Version	NM_001271707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554048000000
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR157266.1, AK298463.1 and BC015715.1. Transcript Variant: This variant (2) lacks an internal exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.196446.1, SRR1660809.143296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACCA AGCAGATCAC TTGCAGATAT GACCACACGA GGCCCTCTGC TGCAGCTGGA 
GGTGCTGTGG GCACCATGGC CCACAGTGTG CCCTCCCCAG CTTTCCACAG TCCTCACCCT 
CCTTCCGAGG TCACTGCATC CATTGTGAAA ACTAACTCAC ATGAACCCGG AAAGCGTGAA 
AAGAGAACAT TGGTTCTTAG AGACCGAAAT CTCTCTGGCA TGGCTGAAAG GAAGACCCAG 
CCGAGCATGG TGAGTAATCC AGGCAGCTGC AGCGACCCCC AGCCCAGCCC CGAGATGAAG 
CCGCATTCCT ACCTGGATGC CATCAGGAGT GGCCTTGATG ACGTGGAGGC CAGCAGCTCC 
TACAGCAACG AGCAGCAGCT GTGCCCCTAC GCAGCTGCTG GGGAGTGCCG GTTTGGGGAT 
GCCTGTGTCT ACCTGCACGG GGAGGTGTGT GAAATCTGTA GGCTGCAAGT CTTGCACCCA 
TTCGACCCAG AGCAGAGGAA GGCTCATGAA AAGATCTGCA TGTTGACGTT CGAACACGAG 
ATGGAAAAGG CCTTTGCCTT CCAGGCAAGC CAGGACAAAG TGTGCAGTAT CTGCATGGAA 
GTGATCCTGG AGAAGGCCTC TGCTTCTGAG AGGAGATTTG GGATTCTCTC CAATTGCAAT 
CACACGTACT GTTTGTCCTG CATCCGGCAG TGGCGGTGTG CCAAACAGTT TGAAAACCCA 
ATCATTAAGT CTTGTCCAGA ATGCCGTGTG ATATCAGAGT TTGTAATTCC AAGTGTGTAT 
TGGGTGGAAG ATCAGAATAA AAAGAACGAG TTGATTGAAG CTTTCAAACA GGGGATGGGG 
AAAAAAGCCT GTAAATACTT TGAGCAAGGC AAGGGGACCT GCCCATTTGG AAGCAAATGT 
CTTTATCGCC ATGCTTACCC CGATGGGCGG CTAGCAGAGC CTGAGAAACC TCGGAAACAG 
CTCAGTTCTC AAGGCACTGT GAGGTTCTTT AATTCAGTGC GGCTCTGGGA TTTCATCGAG 
AACCGAGAAA GCCGGCATGT CCCCAACAAT GAAGATGTCG ACATGACAGA GCTCGGGGAC 
CTCTTCATGC ACCTTTCTGG AGTGGAATCA TCAGAACCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258636.1
CDS	138..1259
Translation	MSTKQITCRYDHTRPSAAAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	NM_001271707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271707.1

ORF Insert Sequence:

ATGAGCACCA AGCAGATCAC TTGCAGATAT GACCACACGA GGCCCTCTGC TGCAGCTGGA 
GGTGCTGTGG GCACCATGGC CCACAGTGTG CCCTCCCCAG CTTTCCACAG TCCTCACCCT 
CCTTCCGAGG TCACTGCATC CATTGTGAAA ACTAACTCAC ATGAACCCGG AAAGCGTGAA 
AAGAGAACAT TGGTTCTTAG AGACCGAAAT CTCTCTGGCA TGGCTGAAAG GAAGACCCAG 
CCGAGCATGG TGAGTAATCC AGGCAGCTGC AGCGACCCCC AGCCCAGCCC CGAGATGAAG 
CCGCATTCCT ACCTGGATGC CATCAGGAGT GGCCTTGATG ACGTGGAGGC CAGCAGCTCC 
TACAGCAACG AGCAGCAGCT GTGCCCCTAC GCAGCTGCTG GGGAGTGCCG GTTTGGGGAT 
GCCTGTGTCT ACCTGCACGG GGAGGTGTGT GAAATCTGTA GGCTGCAAGT CTTGCACCCA 
TTCGACCCAG AGCAGAGGAA GGCTCATGAA AAGATCTGCA TGTTGACGTT CGAACACGAG 
ATGGAAAAGG CCTTTGCCTT CCAGGCAAGC CAGGACAAAG TGTGCAGTAT CTGCATGGAA 
GTGATCCTGG AGAAGGCCTC TGCTTCTGAG AGGAGATTTG GGATTCTCTC CAATTGCAAT 
CACACGTACT GTTTGTCCTG CATCCGGCAG TGGCGGTGTG CCAAACAGTT TGAAAACCCA 
ATCATTAAGT CTTGTCCAGA ATGCCGTGTG ATATCAGAGT TTGTAATTCC AAGTGTGTAT 
TGGGTGGAAG ATCAGAATAA AAAGAACGAG TTGATTGAAG CTTTCAAACA GGGGATGGGG 
AAAAAAGCCT GTAAATACTT TGAGCAAGGC AAGGGGACCT GCCCATTTGG AAGCAAATGT 
CTTTATCGCC ATGCTTACCC CGATGGGCGG CTAGCAGAGC CTGAGAAACC TCGGAAACAG 
CTCAGTTCTC AAGGCACTGT GAGGTTCTTT AATTCAGTGC GGCTCTGGGA TTTCATCGAG 
AACCGAGAAA GCCGGCATGT CCCCAACAAT GAAGATGTCG ACATGACAGA GCTCGGGGAC 
CTCTTCATGC ACCTTTCTGG AGTGGAATCA TCAGAACCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MKRN2 ( NM_014160.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054879.3 Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14084
Clone ID Related Accession (Same CDS sequence)	NM_014160.4 , NM_014160.5
Accession Version	NM_014160.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015715.1 and AK298463.1. On Nov 23, 2018 this sequence version replaced NM_014160.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC015715.1, SRR1803612.263536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000170447.12/ ENSP00000170447.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCACCA AGCAGATCAC TTGCAGGTAT TTTATGCATG GTGTGTGTCG GGAAGGAAGT 
CAGTGCCTAT TCTCACATGA CTTGGCAAAC AGCAAACCGT CCACCATCTG CAAGTACTAC 
CAGAAGGGCT ACTGTGCCTA TGGAACTCGG TGCAGATATG ACCACACGAG GCCCTCTGCT 
GCAGCTGGAG GTGCTGTGGG CACCATGGCC CACAGTGTGC CCTCCCCAGC TTTCCACAGT 
CCTCACCCTC CTTCCGAGGT CACTGCATCC ATTGTGAAAA CTAACTCACA TGAACCCGGA 
AAGCGTGAAA AGAGAACATT GGTTCTTAGA GACCGAAATC TCTCTGGCAT GGCTGAAAGG 
AAGACCCAGC CGAGCATGGT GAGTAATCCA GGCAGCTGCA GCGACCCCCA GCCCAGCCCC 
GAGATGAAGC CGCATTCCTA CCTGGATGCC ATCAGGAGTG GCCTTGATGA CGTGGAGGCC 
AGCAGCTCCT ACAGCAACGA GCAGCAGCTG TGCCCCTACG CAGCTGCTGG GGAGTGCCGG 
TTTGGGGATG CCTGTGTCTA CCTGCACGGG GAGGTGTGTG AAATCTGTAG GCTGCAAGTC 
TTGCACCCAT TCGACCCAGA GCAGAGGAAG GCTCATGAAA AGATCTGCAT GTTGACGTTC 
GAACACGAGA TGGAAAAGGC CTTTGCCTTC CAGGCAAGCC AGGACAAAGT GTGCAGTATC 
TGCATGGAAG TGATCCTGGA GAAGGCCTCT GCTTCTGAGA GGAGATTTGG GATTCTCTCC 
AATTGCAATC ACACGTACTG TTTGTCCTGC ATCCGGCAGT GGCGGTGTGC CAAACAGTTT 
GAAAACCCAA TCATTAAGTC TTGTCCAGAA TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTGGAAGA TCAGAATAAA AAGAACGAGT TGATTGAAGC TTTCAAACAG 
GGGATGGGGA AAAAAGCCTG TAAATACTTT GAGCAAGGCA AGGGGACCTG CCCATTTGGA 
AGCAAATGTC TTTATCGCCA TGCTTACCCC GATGGGCGGC TAGCAGAGCC TGAGAAACCT 
CGGAAACAGC TCAGTTCTCA AGGCACTGTG AGGTTCTTTA ATTCAGTGCG GCTCTGGGAT 
TTCATCGAGA ACCGAGAAAG CCGGCATGTC CCCAACAATG AAGATGTCGA CATGACAGAG 
CTCGGGGACC TCTTCATGCA CCTTTCTGGA GTGGAATCAT CAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054879.3
CDS	65..1315
Translation	MSTKQITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDHTRPSAAAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP

Target ORF information:

RefSeq Version	NM_014160.5
Organism	Homo sapiens(human)
Definition	Homo sapiens makorin ring finger protein 2 (MKRN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014160.5

ORF Insert Sequence:

ATGAGCACCA AGCAGATCAC TTGCAGGTAT TTTATGCATG GTGTGTGTCG GGAAGGAAGT 
CAGTGCCTAT TCTCACATGA CTTGGCAAAC AGCAAACCGT CCACCATCTG CAAGTACTAC 
CAGAAGGGCT ACTGTGCCTA TGGAACTCGG TGCAGATATG ACCACACGAG GCCCTCTGCT 
GCAGCTGGAG GTGCTGTGGG CACCATGGCC CACAGTGTGC CCTCCCCAGC TTTCCACAGT 
CCTCACCCTC CTTCCGAGGT CACTGCATCC ATTGTGAAAA CTAACTCACA TGAACCCGGA 
AAGCGTGAAA AGAGAACATT GGTTCTTAGA GACCGAAATC TCTCTGGCAT GGCTGAAAGG 
AAGACCCAGC CGAGCATGGT GAGTAATCCA GGCAGCTGCA GCGACCCCCA GCCCAGCCCC 
GAGATGAAGC CGCATTCCTA CCTGGATGCC ATCAGGAGTG GCCTTGATGA CGTGGAGGCC 
AGCAGCTCCT ACAGCAACGA GCAGCAGCTG TGCCCCTACG CAGCTGCTGG GGAGTGCCGG 
TTTGGGGATG CCTGTGTCTA CCTGCACGGG GAGGTGTGTG AAATCTGTAG GCTGCAAGTC 
TTGCACCCAT TCGACCCAGA GCAGAGGAAG GCTCATGAAA AGATCTGCAT GTTGACGTTC 
GAACACGAGA TGGAAAAGGC CTTTGCCTTC CAGGCAAGCC AGGACAAAGT GTGCAGTATC 
TGCATGGAAG TGATCCTGGA GAAGGCCTCT GCTTCTGAGA GGAGATTTGG GATTCTCTCC 
AATTGCAATC ACACGTACTG TTTGTCCTGC ATCCGGCAGT GGCGGTGTGC CAAACAGTTT 
GAAAACCCAA TCATTAAGTC TTGTCCAGAA TGCCGTGTGA TATCAGAGTT TGTAATTCCA 
AGTGTGTATT GGGTGGAAGA TCAGAATAAA AAGAACGAGT TGATTGAAGC TTTCAAACAG 
GGGATGGGGA AAAAAGCCTG TAAATACTTT GAGCAAGGCA AGGGGACCTG CCCATTTGGA 
AGCAAATGTC TTTATCGCCA TGCTTACCCC GATGGGCGGC TAGCAGAGCC TGAGAAACCT 
CGGAAACAGC TCAGTTCTCA AGGCACTGTG AGGTTCTTTA ATTCAGTGCG GCTCTGGGAT 
TTCATCGAGA ACCGAGAAAG CCGGCATGTC CCCAACAATG AAGATGTCGA CATGACAGAG 
CTCGGGGACC TCTTCATGCA CCTTTCTGGA GTGGAATCAT CAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway.
Journal of experimental & clinical cancer research : CR37(1)189(2018 Aug)
Jiang J,Xu Y,Ren H,Wudu M,Wang Q,Song X,Su H,Jiang X,Jiang L,Qiu X

MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
Scientific reports746097(2017 04)
Shin C,Ito Y,Ichikawa S,Tokunaga M,Sakata-Sogawa K,Tanaka T

Ubiquitous expression of MAKORIN-2 in normal and malignant hematopoietic cells and its growth promoting activity.
PloS one9(3)e92706(2014)
Lee KY,Chan KY,Tsang KS,Chen YC,Kung HF,Ng PC,Li CK,Leung KT,Li K

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
BMC genomics11721(2010 Dec)
Böhne A,Darras A,D'Cotta H,Baroiller JF,Galiana-Arnoux D,Volff JN

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

MKRN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM