Spag5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag5
Entrez Gene ID	54141
Full Name	sperm associated antigen 5
Synonyms	AI874642,D11Bhm180e,Deepest,MAP126,Mastrin,S17
General protein information	Preferred Names sperm associated antigen 5 Names sperm-associated antigen 5 astrin microtubule-associated protein mitotic spindle-associated protein p126
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 46.74 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533747.4	XP_006533810.1	sperm-associated antigen 5 isoform X1
XM_006533747.3	XP_006533810.1	sperm-associated antigen 5 isoform X1
NM_017407.3	NP_059103.1	sperm-associated antigen 5
XM_006533748.1	XP_006533811.1	sperm-associated antigen 5 isoform X2
NM_017407.2	NP_059103.1	sperm-associated antigen 5

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	SPAG5	XP_001108753.2
Canis lupus familiaris (dog)	SPAG5	XP_537743.4
Homo sapiens (human)	SPAG5	NP_006452.3
Bos taurus (cattle)	SPAG5	NP_001192478.1
Mus musculus (house mouse)	Spag5	NP_059103.1
Rattus norvegicus (Norway rat)	Spag5	NP_001037689.1
Gallus gallus (chicken)	SPAG5	XP_004946625.1

Gene Ontology
Bibliography

Related articles in PubMed

Single-cell transcriptomic analysis of mouse neocortical development.
Loo L, Simon JM, Xing L, McCoy ES, Niehaus JK, Guo J, Anton ES, Zylka MJ
Nature communications10(1)134(2019 01)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

The mitotic spindle protein SPAG5/Astrin connects to the Usher protein network postmitotically.
Kersten FF, van Wijk E, Hetterschijt L, Bauβ K, Peters TA, Aslanyan MG, van der Zwaag B, Wolfrum U, Keunen JE, Roepman R, Kremer H
Cilia1(1)2(2012 Apr)

The role of Pax6 in regulating the orientation and mode of cell division of progenitors in the mouse cerebral cortex.
Asami M, Pilz GA, Ninkovic J, Godinho L, Schroeder T, Huttner WB, Götz M
Development (Cambridge, England)138(23)5067-78(2011 Dec)

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip.
Machold R, Klein C, Fishell G
Gene expression patterns : GEP11(5-6)349-59(2011 Jun-Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spag5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu46620	XM_006533748.1 Latest version!	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu10784	NM_017407.3 Latest version!	Mus musculus sperm associated antigen 5 (Spag5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu46619	XM_006533747.4 Latest version!	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu46619	XM_006533747.3	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu10784	NM_017407.2	Mus musculus sperm associated antigen 5 (Spag5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag5 ( XM_006533748.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533811.1 Mus musculus sperm associated antigen 5 (Spag5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu46620
Clone ID Related Accession (Same CDS sequence)	XM_006533748.1
Accession Version	XM_006533748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAT TCAGAGGCCT TCTACAGGAG GCACAGACCC AACTGATAGG GCTTCACACA 
GAACAAAAGG AGCTGGCTCA GCAGACAGTG AGTCTGAGTT CTGCCTTGCA GCAGGACTGG 
ACATCAGTCC AATTGAATTA TGGAATATGG GCAGCTTTGC TGAGTTGGTC TCGAGAACTC 
ACCAAGAAAC TCACAGCCAA GAGCCGGCAG GCCCTTCAGG AACGTGATGC TGCAATTGAA 
GAAAAGAAGC AGGTTGTGAA GGAAGTGGAA CAAGTCTCTG CCCATTTAGA GGACTGTAAA 
GGTCAAATCG AACAACTGAA GTTGGAAAAT AGTCGCCTTA CTGCAGATCT CTCGGCTCAG 
CTGCAGATTC TGACTAGCAC AGAGAGTCAG CTAAAGGAAG TACGGAGTCA GCATTCCCGC 
TGTGTCCAGG ACCTGGCCGT GAAGGATGAA TTGCTGTGTC AACTTACCCA GAGCAACAAG 
GAGCAGGCTA CTCAATGGCA AAAGGAAGAA ATGGAACTGA AACACATACA AGCAGAACTG 
CTACAGCAGC AGGCTGTGTT GGCTAAGGAG GTCCAGGACC TGAGGGAGAC TGTGGAGTTT 
ATAGATGAAG AAAGTCAAGT TGCTCACCGG GAGCTGGGCC AGATTGAGAG TCAGTTGAAA 
GTCACGCTAG AGTTACTGCG GGAGCGCAGC CTGCAGTGTG AGACCCTCAG GGACACTGTG 
GACAGCCTGA GGGCTGAGCT GGCCAGCACT GAAGCAAAGC ATGAGAAACA GGCCCTAGAG 
AAGACACACC AGCATTCTCA AGAGCTGCGG CTACTGGCTG AGCAGCTGCA GAGTCTCACC 
CTCTTCTTAC AGGCAAAACT CAAGGAGAAC AAGGCTGAAT CAGAGATCAT TCTGCCCAGC 
ACAGGCTCTG CTCCAGCCCA GGAACACCCT CTGTCCAATG ACAGCAGCAT CTCAGAACAG 
ACCCCGACAG CAGCAGTAGA TGAAGTGCCA GAACCAGCTC CTGTGCCATT GCTTGGAAGT 
GTTAAGAGTG CTTTCACCCG AGTAGCCTCA ATGGCTTCCT TTCAGCCTAC AGAGACCCCA 
GACTTGGAGA AGAGCCTGGC AGAAATGAGT ACTGTGTTAC AAGAGCTTAA GAGCCTGTGT 
TCCCTGCTGC AAGAGTCTAA AGAGGAGGCC ACTGGGGTCC TGCAGAGGGA AATCTGTGAA 
CTACACTCGA GACTACAGGC CCAAGAAGAA GAGCATCAGG AAGCCCTGAA GGCAAAGGAA 
GCAGACATGG AGAAGCTGAA CCAGGCCTTG TGCTTGCTCC GCAAGAATGA GAAGGAGCTC 
CTGGAAGTGA TACAGAAGCA GAACGAGAAG ATCCTGGGGC AAATAGACAA GAGCGGCCAG 
CTCATAAACC TCAGAGAGGA GGTGACCCAG CTCACACAGT CACTTCGGCG TGCAGAGACA 
GAGACTAAAG TGCTCCAGGA AGCCCTGGAA GGCCAGCTAG ATCCCAGCTG CCAGCTGATG 
GCTACTAACT GGATCCAGGA AAAAGTGTTT CTCTCACAGG AGGTGAGCAA GCTGAGGGTT 
ATGTTCCTGG AGATGAAAAC TGAGAAGGAA CAGCTGATGG ACAAGTATCT GAGCCATAGG 
CACATCCTGG AGGAGAATCT TCGGCGCTCT GACACAGAGT TAAAGAAACT CGATGACACA 
ATTCAGCATG TCTATGAGAC TCTGTTGTCT ATCCCAGAGA CTATGAAGAG TTGCAAGGAG 
TTACAAGGAT TGCTAGAATT TCTGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533811.1
CDS	286..2055
Translation	MQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLINLREEVTQLTQSLRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLSQEVSKLRVMFLEMKTEKEQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHVYETLLSIPETMKSCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_006533748.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533748.1

ORF Insert Sequence:

ATGCAGGAAT TCAGAGGCCT TCTACAGGAG GCACAGACCC AACTGATAGG GCTTCACACA 
GAACAAAAGG AGCTGGCTCA GCAGACAGTG AGTCTGAGTT CTGCCTTGCA GCAGGACTGG 
ACATCAGTCC AATTGAATTA TGGAATATGG GCAGCTTTGC TGAGTTGGTC TCGAGAACTC 
ACCAAGAAAC TCACAGCCAA GAGCCGGCAG GCCCTTCAGG AACGTGATGC TGCAATTGAA 
GAAAAGAAGC AGGTTGTGAA GGAAGTGGAA CAAGTCTCTG CCCATTTAGA GGACTGTAAA 
GGTCAAATCG AACAACTGAA GTTGGAAAAT AGTCGCCTTA CTGCAGATCT CTCGGCTCAG 
CTGCAGATTC TGACTAGCAC AGAGAGTCAG CTAAAGGAAG TACGGAGTCA GCATTCCCGC 
TGTGTCCAGG ACCTGGCCGT GAAGGATGAA TTGCTGTGTC AACTTACCCA GAGCAACAAG 
GAGCAGGCTA CTCAATGGCA AAAGGAAGAA ATGGAACTGA AACACATACA AGCAGAACTG 
CTACAGCAGC AGGCTGTGTT GGCTAAGGAG GTCCAGGACC TGAGGGAGAC TGTGGAGTTT 
ATAGATGAAG AAAGTCAAGT TGCTCACCGG GAGCTGGGCC AGATTGAGAG TCAGTTGAAA 
GTCACGCTAG AGTTACTGCG GGAGCGCAGC CTGCAGTGTG AGACCCTCAG GGACACTGTG 
GACAGCCTGA GGGCTGAGCT GGCCAGCACT GAAGCAAAGC ATGAGAAACA GGCCCTAGAG 
AAGACACACC AGCATTCTCA AGAGCTGCGG CTACTGGCTG AGCAGCTGCA GAGTCTCACC 
CTCTTCTTAC AGGCAAAACT CAAGGAGAAC AAGGCTGAAT CAGAGATCAT TCTGCCCAGC 
ACAGGCTCTG CTCCAGCCCA GGAACACCCT CTGTCCAATG ACAGCAGCAT CTCAGAACAG 
ACCCCGACAG CAGCAGTAGA TGAAGTGCCA GAACCAGCTC CTGTGCCATT GCTTGGAAGT 
GTTAAGAGTG CTTTCACCCG AGTAGCCTCA ATGGCTTCCT TTCAGCCTAC AGAGACCCCA 
GACTTGGAGA AGAGCCTGGC AGAAATGAGT ACTGTGTTAC AAGAGCTTAA GAGCCTGTGT 
TCCCTGCTGC AAGAGTCTAA AGAGGAGGCC ACTGGGGTCC TGCAGAGGGA AATCTGTGAA 
CTACACTCGA GACTACAGGC CCAAGAAGAA GAGCATCAGG AAGCCCTGAA GGCAAAGGAA 
GCAGACATGG AGAAGCTGAA CCAGGCCTTG TGCTTGCTCC GCAAGAATGA GAAGGAGCTC 
CTGGAAGTGA TACAGAAGCA GAACGAGAAG ATCCTGGGGC AAATAGACAA GAGCGGCCAG 
CTCATAAACC TCAGAGAGGA GGTGACCCAG CTCACACAGT CACTTCGGCG TGCAGAGACA 
GAGACTAAAG TGCTCCAGGA AGCCCTGGAA GGCCAGCTAG ATCCCAGCTG CCAGCTGATG 
GCTACTAACT GGATCCAGGA AAAAGTGTTT CTCTCACAGG AGGTGAGCAA GCTGAGGGTT 
ATGTTCCTGG AGATGAAAAC TGAGAAGGAA CAGCTGATGG ACAAGTATCT GAGCCATAGG 
CACATCCTGG AGGAGAATCT TCGGCGCTCT GACACAGAGT TAAAGAAACT CGATGACACA 
ATTCAGCATG TCTATGAGAC TCTGTTGTCT ATCCCAGAGA CTATGAAGAG TTGCAAGGAG 
TTACAAGGAT TGCTAGAATT TCTGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( NM_017407.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059103.1 Mus musculus sperm associated antigen 5 (Spag5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10784
Clone ID Related Accession (Same CDS sequence)	NM_017407.3 , NM_017407.2
Accession Version	NM_017407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568563200000
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591070.19. On May 1, 2018 this sequence version replaced NM_017407.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076619.1, SRR1660815.255395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAAACCTC 
AGAGAGGAGG TGACCCAGCT CACACAGTCA CTTCGGCGTG CAGAGACAGA GACTAAAGTG 
CTCCAGGAAG CCCTGGAAGG CCAGCTAGAT CCCAGCTGCC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTTTCT CTCACAGGAG GTGAGCAAGC TGAGGGTTAT GTTCCTGGAG 
ATGAAAACTG AGAAGGAACA GCTGATGGAC AAGTATCTGA GCCATAGGCA CATCCTGGAG 
GAGAATCTTC GGCGCTCTGA CACAGAGTTA AAGAAACTCG ATGACACAAT TCAGCATGTC 
TATGAGACTC TGTTGTCTAT CCCAGAGACT ATGAAGAGTT GCAAGGAGTT ACAAGGATTG 
CTAGAATTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059103.1
CDS	70..3567
Translation	MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDFINTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLGQQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDAPVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANAEAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQDAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLSHQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLHLSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFRAHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLINLREEVTQLTQSLRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLSQEVSKLRVMFLEMKTEKEQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHVYETLLSIPETMKSCKELQGLLEFLS

Target ORF information:

RefSeq Version	NM_017407.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 5 (Spag5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017407.3

ORF Insert Sequence:

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAAACCTC 
AGAGAGGAGG TGACCCAGCT CACACAGTCA CTTCGGCGTG CAGAGACAGA GACTAAAGTG 
CTCCAGGAAG CCCTGGAAGG CCAGCTAGAT CCCAGCTGCC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTTTCT CTCACAGGAG GTGAGCAAGC TGAGGGTTAT GTTCCTGGAG 
ATGAAAACTG AGAAGGAACA GCTGATGGAC AAGTATCTGA GCCATAGGCA CATCCTGGAG 
GAGAATCTTC GGCGCTCTGA CACAGAGTTA AAGAAACTCG ATGACACAAT TCAGCATGTC 
TATGAGACTC TGTTGTCTAT CCCAGAGACT ATGAAGAGTT GCAAGGAGTT ACAAGGATTG 
CTAGAATTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( NM_017407.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059103.1 Mus musculus sperm associated antigen 5 (Spag5), mRNA.

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CloneID	OMu10784
Clone ID Related Accession (Same CDS sequence)	NM_017407.3 , NM_017407.2
Accession Version	NM_017407.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591070.19. On Aug 13, 2009 this sequence version replaced NM_017407.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052672.1, AK076619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAAACCTC 
AGAGAGGAGG TGACCCAGCT CACACAGTCA CTTCGGCGTG CAGAGACAGA GACTAAAGTG 
CTCCAGGAAG CCCTGGAAGG CCAGCTAGAT CCCAGCTGCC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTTTCT CTCACAGGAG GTGAGCAAGC TGAGGGTTAT GTTCCTGGAG 
ATGAAAACTG AGAAGGAACA GCTGATGGAC AAGTATCTGA GCCATAGGCA CATCCTGGAG 
GAGAATCTTC GGCGCTCTGA CACAGAGTTA AAGAAACTCG ATGACACAAT TCAGCATGTC 
TATGAGACTC TGTTGTCTAT CCCAGAGACT ATGAAGAGTT GCAAGGAGTT ACAAGGATTG 
CTAGAATTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059103.1
CDS	70..3567
Translation	MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDFINTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLGQQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDAPVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANAEAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQDAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLSHQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLHLSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFRAHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLINLREEVTQLTQSLRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLSQEVSKLRVMFLEMKTEKEQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHVYETLLSIPETMKSCKELQGLLEFLS

Target ORF information:

RefSeq Version	NM_017407.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 5 (Spag5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017407.2

ORF Insert Sequence:

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAAACCTC 
AGAGAGGAGG TGACCCAGCT CACACAGTCA CTTCGGCGTG CAGAGACAGA GACTAAAGTG 
CTCCAGGAAG CCCTGGAAGG CCAGCTAGAT CCCAGCTGCC AGCTGATGGC TACTAACTGG 
ATCCAGGAAA AAGTGTTTCT CTCACAGGAG GTGAGCAAGC TGAGGGTTAT GTTCCTGGAG 
ATGAAAACTG AGAAGGAACA GCTGATGGAC AAGTATCTGA GCCATAGGCA CATCCTGGAG 
GAGAATCTTC GGCGCTCTGA CACAGAGTTA AAGAAACTCG ATGACACAAT TCAGCATGTC 
TATGAGACTC TGTTGTCTAT CCCAGAGACT ATGAAGAGTT GCAAGGAGTT ACAAGGATTG 
CTAGAATTTC TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_006533747.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533810.1 Mus musculus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

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CloneID	OMu46619
Clone ID Related Accession (Same CDS sequence)	XM_006533747.4 , XM_006533747.3
Accession Version	XM_006533747.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533747.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAGTACGT 
TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533810.1
CDS	75..3203
Translation	MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDFINTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLGQQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDAPVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANAEAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQDAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLSHQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLHLSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFRAHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLIVRFS

Target ORF information:

RefSeq Version	XM_006533747.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533747.4

ORF Insert Sequence:

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAGTACGT 
TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_006533747.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533810.1 Mus musculus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

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CloneID	OMu46619
Clone ID Related Accession (Same CDS sequence)	XM_006533747.4 , XM_006533747.3
Accession Version	XM_006533747.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAGTACGT 
TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533810.1
CDS	101..3229
Translation	MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDFINTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLGQQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDAPVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANAEAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQDAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLSHQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLHLSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFRAHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLIVRFS

Target ORF information:

RefSeq Version	XM_006533747.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533747.3

ORF Insert Sequence:

ATGTGGAGGG TGAAAACACT GAATCTCGGT TTGTCGCCTT CGCCCCAAAA GGGGAAGCCA 
GCTATGAGTA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC CGATTCAGGG 
AAAGGTCCCT CTATGATCTC CGCGCTGACC CCATACCTGT GCAGGCTGGA GCTGAAGGAA 
AGATGCAACA ACTCATCTCC AGTGGATTTT ATCAATACTG AGAACAACTT CCTTTCAGAA 
CAGTTCAGCC ATCCTTCAAC GCACATAGAA GCTTGCCAGC GTGAATCCGA TCCAACTCCT 
GAAAGCAACT CTCTTTTCCA TACTTTGGAG GAAGCAATAG AAACAGTGGA TGACTTTGTC 
GTGGACCCAA GAGATGATAG TATAGTGGAG TCCATGGTTC TTCTGCCCTT TTCATTAGGG 
CAGCAGCAAG ACCTGATGCT CCAGGCTCAC TTAGATACCA CAGCAGAGAG AACTAAAAGC 
TCTCTAAATG AATCTTTGGG GCTAGAAGAT CTGGTGGGGA AGGAGGTGGC ACCTTGTGTG 
GAAGACAGCC TAACAGAAAT TGTTGCTATT AGGCCCGAGC AACCTACATT TCAGGACCCT 
CCGTTAGGAC CCAGTGACAC TGAAGATGCA CCTGTGGACT TAGTTCCTTC TGAAAATGTC 
CTGAATTTCT CTCTGGCTCG TCTTTCTCCT TCAGCTGTCC TGGCCCAGGA TTTCTCTGTC 
GATCATGTCG ATCCAGGGGA GGAAACCGTA GAGAACAGAG TCCTACAGGA AATGGAAACG 
AGCTTCCCCA CATTCCCTGA GGAGGCCGAA TTGGGAGACC AAGCACCTGC TGCAAATGCA 
GAAGCCGTCT CACCCTTGTA CCTGACATCA AGTCTAGTGG AGATGGGGCC CCGGGAAGCC 
CCAGGCCCAA CAGTAGAAGA CGCCAGTAGG ATTCCTGGCC TTGAGTCAGA GACTTGGATG 
TCCCCATTGG CCTGGCTAGA AAAGGGTGTG AATACATCGG TCATGCTACA AAACCTCCGC 
CAGAGCTTAT CCTTTTCTTC TGTGCTTCAG GATGCTGCCG TTGGCAACAC ACCCCTCGCC 
ACGTGTTCTG TGGGCACTTC CTTTACTCCT CCAGCACCAC TGGAGGTAGG CACCAAGGAC 
AGTACTTCAG AGACAGAGCG CCTCCTCTTG GGCTGCCGTC CTCCAGATCT GGCTACCTTG 
TCCCGACACG ACTTGGAAGA GAACCTGTTG AACTCTCTTG TCTTGCTGGA AGTTCTTTCC 
CACCAGCTAC AAGCCTGGAA GAGCCAGTTG ACTGTCCCTC ACCGGGAAGC TCGAGACAGT 
AGTACACAGA CTGACAGCTC TCCTTGTGGG GTCACTAAGA CACCTAAACA TCTTCAGGAC 
AGCAAGGAGA TTAGACAGGC TCTACTGCAA GCCAGGAATG TCATGCAATC ATGGGGTCTA 
GTCTCTGGAG ACCTGTTGTC CTTGCTTCAC CTGTCCCTAA CACACGTGCA AGAAGGCAGA 
GTGACAGTGA GTCAGGAGTC TCAGCGGTCA AAAACCCTGG TTTCCTCCTG TTCTCGTGTG 
CTGAAGAAAT TGAAGGCAAA GCTGCAGAGC CTCAAAACAG AATGTGAGGA GGCAAGGCAC 
AGCAAGGAAA TGGCCCTTAA AGGCAAGGCT GCGGCAGAGG CAGTACTGGA GGCTTTCCGT 
GCACATGCCA GCCAGCGCAT CAGCCAACTG GAACAGGGTC TCACATCTAT GCAGGAATTC 
AGAGGCCTTC TACAGGAGGC ACAGACCCAA CTGATAGGGC TTCACACAGA ACAAAAGGAG 
CTGGCTCAGC AGACAGTGAG TCTGAGTTCT GCCTTGCAGC AGGACTGGAC ATCAGTCCAA 
TTGAATTATG GAATATGGGC AGCTTTGCTG AGTTGGTCTC GAGAACTCAC CAAGAAACTC 
ACAGCCAAGA GCCGGCAGGC CCTTCAGGAA CGTGATGCTG CAATTGAAGA AAAGAAGCAG 
GTTGTGAAGG AAGTGGAACA AGTCTCTGCC CATTTAGAGG ACTGTAAAGG TCAAATCGAA 
CAACTGAAGT TGGAAAATAG TCGCCTTACT GCAGATCTCT CGGCTCAGCT GCAGATTCTG 
ACTAGCACAG AGAGTCAGCT AAAGGAAGTA CGGAGTCAGC ATTCCCGCTG TGTCCAGGAC 
CTGGCCGTGA AGGATGAATT GCTGTGTCAA CTTACCCAGA GCAACAAGGA GCAGGCTACT 
CAATGGCAAA AGGAAGAAAT GGAACTGAAA CACATACAAG CAGAACTGCT ACAGCAGCAG 
GCTGTGTTGG CTAAGGAGGT CCAGGACCTG AGGGAGACTG TGGAGTTTAT AGATGAAGAA 
AGTCAAGTTG CTCACCGGGA GCTGGGCCAG ATTGAGAGTC AGTTGAAAGT CACGCTAGAG 
TTACTGCGGG AGCGCAGCCT GCAGTGTGAG ACCCTCAGGG ACACTGTGGA CAGCCTGAGG 
GCTGAGCTGG CCAGCACTGA AGCAAAGCAT GAGAAACAGG CCCTAGAGAA GACACACCAG 
CATTCTCAAG AGCTGCGGCT ACTGGCTGAG CAGCTGCAGA GTCTCACCCT CTTCTTACAG 
GCAAAACTCA AGGAGAACAA GGCTGAATCA GAGATCATTC TGCCCAGCAC AGGCTCTGCT 
CCAGCCCAGG AACACCCTCT GTCCAATGAC AGCAGCATCT CAGAACAGAC CCCGACAGCA 
GCAGTAGATG AAGTGCCAGA ACCAGCTCCT GTGCCATTGC TTGGAAGTGT TAAGAGTGCT 
TTCACCCGAG TAGCCTCAAT GGCTTCCTTT CAGCCTACAG AGACCCCAGA CTTGGAGAAG 
AGCCTGGCAG AAATGAGTAC TGTGTTACAA GAGCTTAAGA GCCTGTGTTC CCTGCTGCAA 
GAGTCTAAAG AGGAGGCCAC TGGGGTCCTG CAGAGGGAAA TCTGTGAACT ACACTCGAGA 
CTACAGGCCC AAGAAGAAGA GCATCAGGAA GCCCTGAAGG CAAAGGAAGC AGACATGGAG 
AAGCTGAACC AGGCCTTGTG CTTGCTCCGC AAGAATGAGA AGGAGCTCCT GGAAGTGATA 
CAGAAGCAGA ACGAGAAGAT CCTGGGGCAA ATAGACAAGA GCGGCCAGCT CATAGTACGT 
TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single-cell transcriptomic analysis of mouse neocortical development.
Nature communications10(1)134(2019 01)
Loo L,Simon JM,Xing L,McCoy ES,Niehaus JK,Guo J,Anton ES,Zylka MJ

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

The mitotic spindle protein SPAG5/Astrin connects to the Usher protein network postmitotically.
Cilia1(1)2(2012 Apr)
Kersten FF,van Wijk E,Hetterschijt L,Bauβ K,Peters TA,Aslanyan MG,van der Zwaag B,Wolfrum U,Keunen JE,Roepman R,Kremer H

The role of Pax6 in regulating the orientation and mode of cell division of progenitors in the mouse cerebral cortex.
Development (Cambridge, England)138(23)5067-78(2011 Dec)
Asami M,Pilz GA,Ninkovic J,Godinho L,Schroeder T,Huttner WB,Götz M

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip.
Gene expression patterns : GEP11(5-6)349-59(2011 Jun-Jul)
Machold R,Klein C,Fishell G

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Spag5 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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