Spag5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag5
Entrez Gene ID	252918
Full Name	sperm associated antigen 5
Synonyms	Deepest
General protein information	Preferred Names sperm associated antigen 5 Names sperm-associated antigen 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q25
Summary	C-terminal has two leucine zippers which are involved in sperm tail protein interactions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246951.2	XP_006247013.2	sperm-associated antigen 5 isoform X3
XM_017597035.1	XP_017452524.1	sperm-associated antigen 5 isoform X2
NM_001044224.1	NP_001037689.1	sperm-associated antigen 5
XM_006220734.2	XP_006220796.2	sperm-associated antigen 5 isoform X3
XM_017597034.1	XP_017452523.1	sperm-associated antigen 5 isoform X1
XM_017604093.1	XP_017459582.1	sperm-associated antigen 5 isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	SPAG5	XP_001108753.2
Canis lupus familiaris (dog)	SPAG5	XP_537743.4
Homo sapiens (human)	SPAG5	NP_006452.3
Bos taurus (cattle)	SPAG5	NP_001192478.1
Mus musculus (house mouse)	Spag5	NP_059103.1
Rattus norvegicus (Norway rat)	Spag5	NP_001037689.1
Gallus gallus (chicken)	SPAG5	XP_004946625.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates Astrin at the Mitotic Spindle.
Chu X, Chen X, Wan Q, Zheng Z, Du Q
The Journal of biological chemistry291(38)20055-67(2016 09)

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife4(2015 Aug)

The microtubule-associated protein astrin transgenesis rescues spermatogenesis and renal function in hypogonadic (hgn/hgn) rats.
Katayama K, Yasuda H, Tochigi Y, Suzuki H
Andrology1(2)301-7(2013 Mar)

The role of Pax6 in regulating the orientation and mode of cell division of progenitors in the mouse cerebral cortex.
Asami M, Pilz GA, Ninkovic J, Godinho L, Schroeder T, Huttner WB, Götz M
Development (Cambridge, England)138(23)5067-78(2011 Dec)

The astrin-kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment.
Dunsch AK, Linnane E, Barr FA, Gruneberg U
The Journal of cell biology192(6)959-68(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spag5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa50051	XM_006220734.2 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa50051	XM_006246951.2 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa53954	XM_017597035.1 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa53954	XM_017604093.1 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa09938	NM_001044224.1 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57195	XM_017597034.1 Latest version!	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag5 ( XM_006246951.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247013.2 Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa50051
Clone ID Related Accession (Same CDS sequence)	XM_006246951.2 , XM_006220734.2
Accession Version	XM_006246951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGT 
GGCAACATCC AGGGTCACTG GGCTCGCTGG AAGAGCCTCT GGTGTGGTAT GGGGACCATC 
AGGTCAGGTC TGGAAGAACT GTGGGAACTA CAGAGGCATC AGTGCCTACA TCAGGAGCTC 
CTCCAGCCAG CCCCATTGCT GCTAGAGAAG CCTCTGTCTG AATGGCCAGT GCCTCAGTTC 
ATCAGCCTCT TTCTACCAGA GTTTCCCATG CGGCCTGTTA AAGAGCAGGA GCTGAAGATT 
TTAGGCCTTG TGGCTAAAGG TTCGTTTGGA ACTGTCCTGA AAGTGCTAGA TTGTGCCCAG 
AATGCTGTAT TTGCAGTGAA GGTGGTACCC AAAGTAAAAG TTCTACAGCG GGATACCCTG 
AGGCAGTGCA AGGAGGAGGT TAGCATCCAG CGGCAGATCA ACCATCCTTT TGTACACAGT 
TTGGGGGACA GCTGGCAGGG GAAACGACAC CTCTTTATTA TGTGTAGCTA CTGCAGCATG 
GATCTCTACT CCCTGTGGTC CACTGTTGGT TGTTTTCCAG AGGATTCTGT CCGTCTCTTT 
GCAGCTGAAC TGGTCCTTGT ACTCTGCTAT CTCCATGACT TGGGCATCAT CCATCGTGAT 
GTAAAGATGG AGAATATCCT GCTGGATGAA CGAGGCCATC TGAAAGTTAC AGACTTTGGT 
CTGTCTCGCC GCCTATCCCA GGGAGCACGG GCCTATACTA TCTGTGGCAC TCTTCAATAC 
ATGGCTCCAG AGGTCTTGAG TGGTGGGCCT TACAACCACA CTGCTGACTG GTGGTCCCTG 
GGAGTATTGC TTTTCTCTCT GGCAGCTGGT AAGTTCCCAG TGGCTGCAGA GAGAGATCAT 
TTGACCATGT TGGCAAGTGT GACCCACTGT GGATCTGAGA TCCCAGCGTC TATTAATCAG 
GAGCTCTCAC TCCTGCTCCA TGAGCTCTTA TGCCAGAACC CTCTGTACCG TCTACGATAC 
CTGCATCACT TCCAGGCCCA CCCTTTCTTC CGGGGTGTGG CCTTTGACCC TGAGCTCCTA 
CAGAAGCAGC CAGTGAACTT TGTCATGGAG ACACAAGATA CCTGGCCCAG TCCATTGGAG 
AACATGTCCT TTGAGGACTT CGACTGTGAT CTCGAGTCCT TGGTCCATTC CATCTCTATT 
GACTCTCTAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247013.2
CDS	118..1332
Translation	MGAVSCRQGQHTRVSAPQKGGNIQGHWARWKSLWCGMGTIRSGLEELWELQRHQCLHQELLQPAPLLLEKPLSEWPVPQFISLFLPEFPMRPVKEQELKILGLVAKGSFGTVLKVLDCAQNAVFAVKVVPKVKVLQRDTLRQCKEEVSIQRQINHPFVHSLGDSWQGKRHLFIMCSYCSMDLYSLWSTVGCFPEDSVRLFAAELVLVLCYLHDLGIIHRDVKMENILLDERGHLKVTDFGLSRRLSQGARAYTICGTLQYMAPEVLSGGPYNHTADWWSLGVLLFSLAAGKFPVAAERDHLTMLASVTHCGSEIPASINQELSLLLHELLCQNPLYRLRYLHHFQAHPFFRGVAFDPELLQKQPVNFVMETQDTWPSPLENMSFEDFDCDLESLVHSISIDSLL

Target ORF information:

RefSeq Version	XM_006246951.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246951.2

ORF Insert Sequence:

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGT 
GGCAACATCC AGGGTCACTG GGCTCGCTGG AAGAGCCTCT GGTGTGGTAT GGGGACCATC 
AGGTCAGGTC TGGAAGAACT GTGGGAACTA CAGAGGCATC AGTGCCTACA TCAGGAGCTC 
CTCCAGCCAG CCCCATTGCT GCTAGAGAAG CCTCTGTCTG AATGGCCAGT GCCTCAGTTC 
ATCAGCCTCT TTCTACCAGA GTTTCCCATG CGGCCTGTTA AAGAGCAGGA GCTGAAGATT 
TTAGGCCTTG TGGCTAAAGG TTCGTTTGGA ACTGTCCTGA AAGTGCTAGA TTGTGCCCAG 
AATGCTGTAT TTGCAGTGAA GGTGGTACCC AAAGTAAAAG TTCTACAGCG GGATACCCTG 
AGGCAGTGCA AGGAGGAGGT TAGCATCCAG CGGCAGATCA ACCATCCTTT TGTACACAGT 
TTGGGGGACA GCTGGCAGGG GAAACGACAC CTCTTTATTA TGTGTAGCTA CTGCAGCATG 
GATCTCTACT CCCTGTGGTC CACTGTTGGT TGTTTTCCAG AGGATTCTGT CCGTCTCTTT 
GCAGCTGAAC TGGTCCTTGT ACTCTGCTAT CTCCATGACT TGGGCATCAT CCATCGTGAT 
GTAAAGATGG AGAATATCCT GCTGGATGAA CGAGGCCATC TGAAAGTTAC AGACTTTGGT 
CTGTCTCGCC GCCTATCCCA GGGAGCACGG GCCTATACTA TCTGTGGCAC TCTTCAATAC 
ATGGCTCCAG AGGTCTTGAG TGGTGGGCCT TACAACCACA CTGCTGACTG GTGGTCCCTG 
GGAGTATTGC TTTTCTCTCT GGCAGCTGGT AAGTTCCCAG TGGCTGCAGA GAGAGATCAT 
TTGACCATGT TGGCAAGTGT GACCCACTGT GGATCTGAGA TCCCAGCGTC TATTAATCAG 
GAGCTCTCAC TCCTGCTCCA TGAGCTCTTA TGCCAGAACC CTCTGTACCG TCTACGATAC 
CTGCATCACT TCCAGGCCCA CCCTTTCTTC CGGGGTGTGG CCTTTGACCC TGAGCTCCTA 
CAGAAGCAGC CAGTGAACTT TGTCATGGAG ACACAAGATA CCTGGCCCAG TCCATTGGAG 
AACATGTCCT TTGAGGACTT CGACTGTGAT CTCGAGTCCT TGGTCCATTC CATCTCTATT 
GACTCTCTAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_006220734.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006220796.2 Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa50051
Clone ID Related Accession (Same CDS sequence)	XM_006246951.2 , XM_006220734.2
Accession Version	XM_006220734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006220734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGT 
GGCAACATCC AGGGTCACTG GGCTCGCTGG AAGAGCCTCT GGTGTGGTAT GGGGACCATC 
AGGTCAGGTC TGGAAGAACT GTGGGAACTA CAGAGGCATC AGTGCCTACA TCAGGAGCTC 
CTCCAGCCAG CCCCATTGCT GCTAGAGAAG CCTCTGTCTG AATGGCCAGT GCCTCAGTTC 
ATCAGCCTCT TTCTACCAGA GTTTCCCATG CGGCCTGTTA AAGAGCAGGA GCTGAAGATT 
TTAGGCCTTG TGGCTAAAGG TTCGTTTGGA ACTGTCCTGA AAGTGCTAGA TTGTGCCCAG 
AATGCTGTAT TTGCAGTGAA GGTGGTACCC AAAGTAAAAG TTCTACAGCG GGATACCCTG 
AGGCAGTGCA AGGAGGAGGT TAGCATCCAG CGGCAGATCA ACCATCCTTT TGTACACAGT 
TTGGGGGACA GCTGGCAGGG GAAACGACAC CTCTTTATTA TGTGTAGCTA CTGCAGCATG 
GATCTCTACT CCCTGTGGTC CACTGTTGGT TGTTTTCCAG AGGATTCTGT CCGTCTCTTT 
GCAGCTGAAC TGGTCCTTGT ACTCTGCTAT CTCCATGACT TGGGCATCAT CCATCGTGAT 
GTAAAGATGG AGAATATCCT GCTGGATGAA CGAGGCCATC TGAAAGTTAC AGACTTTGGT 
CTGTCTCGCC GCCTATCCCA GGGAGCACGG GCCTATACTA TCTGTGGCAC TCTTCAATAC 
ATGGCTCCAG AGGTCTTGAG TGGTGGGCCT TACAACCACA CTGCTGACTG GTGGTCCCTG 
GGAGTATTGC TTTTCTCTCT GGCAGCTGGT AAGTTCCCAG TGGCTGCAGA GAGAGATCAT 
TTGACCATGT TGGCAAGTGT GACCCACTGT GGATCTGAGA TCCCAGCGTC TATTAATCAG 
GAGCTCTCAC TCCTGCTCCA TGAGCTCTTA TGCCAGAACC CTCTGTACCG TCTACGATAC 
CTGCATCACT TCCAGGCCCA CCCTTTCTTC CGGGGTGTGG CCTTTGACCC TGAGCTCCTA 
CAGAAGCAGC CAGTGAACTT TGTCATGGAG ACACAAGATA CCTGGCCCAG TCCATTGGAG 
AACATGTCCT TTGAGGACTT CGACTGTGAT CTCGAGTCCT TGGTCCATTC CATCTCTATT 
GACTCTCTAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006220796.2
CDS	118..1332
Translation	MGAVSCRQGQHTRVSAPQKGGNIQGHWARWKSLWCGMGTIRSGLEELWELQRHQCLHQELLQPAPLLLEKPLSEWPVPQFISLFLPEFPMRPVKEQELKILGLVAKGSFGTVLKVLDCAQNAVFAVKVVPKVKVLQRDTLRQCKEEVSIQRQINHPFVHSLGDSWQGKRHLFIMCSYCSMDLYSLWSTVGCFPEDSVRLFAAELVLVLCYLHDLGIIHRDVKMENILLDERGHLKVTDFGLSRRLSQGARAYTICGTLQYMAPEVLSGGPYNHTADWWSLGVLLFSLAAGKFPVAAERDHLTMLASVTHCGSEIPASINQELSLLLHELLCQNPLYRLRYLHHFQAHPFFRGVAFDPELLQKQPVNFVMETQDTWPSPLENMSFEDFDCDLESLVHSISIDSLL

Target ORF information:

RefSeq Version	XM_006220734.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006220734.2

ORF Insert Sequence:

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGT 
GGCAACATCC AGGGTCACTG GGCTCGCTGG AAGAGCCTCT GGTGTGGTAT GGGGACCATC 
AGGTCAGGTC TGGAAGAACT GTGGGAACTA CAGAGGCATC AGTGCCTACA TCAGGAGCTC 
CTCCAGCCAG CCCCATTGCT GCTAGAGAAG CCTCTGTCTG AATGGCCAGT GCCTCAGTTC 
ATCAGCCTCT TTCTACCAGA GTTTCCCATG CGGCCTGTTA AAGAGCAGGA GCTGAAGATT 
TTAGGCCTTG TGGCTAAAGG TTCGTTTGGA ACTGTCCTGA AAGTGCTAGA TTGTGCCCAG 
AATGCTGTAT TTGCAGTGAA GGTGGTACCC AAAGTAAAAG TTCTACAGCG GGATACCCTG 
AGGCAGTGCA AGGAGGAGGT TAGCATCCAG CGGCAGATCA ACCATCCTTT TGTACACAGT 
TTGGGGGACA GCTGGCAGGG GAAACGACAC CTCTTTATTA TGTGTAGCTA CTGCAGCATG 
GATCTCTACT CCCTGTGGTC CACTGTTGGT TGTTTTCCAG AGGATTCTGT CCGTCTCTTT 
GCAGCTGAAC TGGTCCTTGT ACTCTGCTAT CTCCATGACT TGGGCATCAT CCATCGTGAT 
GTAAAGATGG AGAATATCCT GCTGGATGAA CGAGGCCATC TGAAAGTTAC AGACTTTGGT 
CTGTCTCGCC GCCTATCCCA GGGAGCACGG GCCTATACTA TCTGTGGCAC TCTTCAATAC 
ATGGCTCCAG AGGTCTTGAG TGGTGGGCCT TACAACCACA CTGCTGACTG GTGGTCCCTG 
GGAGTATTGC TTTTCTCTCT GGCAGCTGGT AAGTTCCCAG TGGCTGCAGA GAGAGATCAT 
TTGACCATGT TGGCAAGTGT GACCCACTGT GGATCTGAGA TCCCAGCGTC TATTAATCAG 
GAGCTCTCAC TCCTGCTCCA TGAGCTCTTA TGCCAGAACC CTCTGTACCG TCTACGATAC 
CTGCATCACT TCCAGGCCCA CCCTTTCTTC CGGGGTGTGG CCTTTGACCC TGAGCTCCTA 
CAGAAGCAGC CAGTGAACTT TGTCATGGAG ACACAAGATA CCTGGCCCAG TCCATTGGAG 
AACATGTCCT TTGAGGACTT CGACTGTGAT CTCGAGTCCT TGGTCCATTC CATCTCTATT 
GACTCTCTAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_017597035.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452524.1 Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa53954
Clone ID Related Accession (Same CDS sequence)	XM_017597035.1 , XM_017604093.1
Accession Version	XM_017597035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGCAG 
GGTGGCAACA TCCAGGGTCA CTGGGCTCGC TGGAAGAGCC TCTGGTGTGG TATGGGGACC 
ATCAGGTCAG GTCTGGAAGA ACTGTGGGAA CTACAGAGGC ATCAGTGCCT ACATCAGGAG 
CTCCTCCAGC CAGCCCCATT GCTGCTAGAG AAGCCTCTGT CTGAATGGCC AGTGCCTCAG 
TTCATCAGCC TCTTTCTACC AGAGTTTCCC ATGCGGCCTG TTAAAGAGCA GGAGCTGAAG 
ATTTTAGGCC TTGTGGCTAA AGGTTCGTTT GGAACTGTCC TGAAAGTGCT AGATTGTGCC 
CAGAATGCTG TATTTGCAGT GAAGGTGGTA CCCAAAGTAA AAGTTCTACA GCGGGATACC 
CTGAGGCAGT GCAAGGAGGA GGTTAGCATC CAGCGGCAGA TCAACCATCC TTTTGTACAC 
AGTTTGGGGG ACAGCTGGCA GGGGAAACGA CACCTCTTTA TTATGTGTAG CTACTGCAGC 
ATGGATCTCT ACTCCCTGTG GTCCACTGTT GGTTGTTTTC CAGAGGATTC TGTCCGTCTC 
TTTGCAGCTG AACTGGTCCT TGTACTCTGC TATCTCCATG ACTTGGGCAT CATCCATCGT 
GATGTAAAGA TGGAGAATAT CCTGCTGGAT GAACGAGGCC ATCTGAAAGT TACAGACTTT 
GGTCTGTCTC GCCGCCTATC CCAGGGAGCA CGGGCCTATA CTATCTGTGG CACTCTTCAA 
TACATGGCTC CAGAGGTCTT GAGTGGTGGG CCTTACAACC ACACTGCTGA CTGGTGGTCC 
CTGGGAGTAT TGCTTTTCTC TCTGGCAGCT GGTAAGTTCC CAGTGGCTGC AGAGAGAGAT 
CATTTGACCA TGTTGGCAAG TGTGACCCAC TGTGGATCTG AGATCCCAGC GTCTATTAAT 
CAGGAGCTCT CACTCCTGCT CCATGAGCTC TTATGCCAGA ACCCTCTGTA CCGTCTACGA 
TACCTGCATC ACTTCCAGGC CCACCCTTTC TTCCGGGGTG TGGCCTTTGA CCCTGAGCTC 
CTACAGAAGC AGCCAGTGAA CTTTGTCATG GAGACACAAG ATACCTGGCC CAGTCCATTG 
GAGAACATGT CCTTTGAGGA CTTCGACTGT GATCTCGAGT CCTTGGTCCA TTCCATCTCT 
ATTGACTCTC TACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452524.1
CDS	118..1335
Translation	MGAVSCRQGQHTRVSAPQKQGGNIQGHWARWKSLWCGMGTIRSGLEELWELQRHQCLHQELLQPAPLLLEKPLSEWPVPQFISLFLPEFPMRPVKEQELKILGLVAKGSFGTVLKVLDCAQNAVFAVKVVPKVKVLQRDTLRQCKEEVSIQRQINHPFVHSLGDSWQGKRHLFIMCSYCSMDLYSLWSTVGCFPEDSVRLFAAELVLVLCYLHDLGIIHRDVKMENILLDERGHLKVTDFGLSRRLSQGARAYTICGTLQYMAPEVLSGGPYNHTADWWSLGVLLFSLAAGKFPVAAERDHLTMLASVTHCGSEIPASINQELSLLLHELLCQNPLYRLRYLHHFQAHPFFRGVAFDPELLQKQPVNFVMETQDTWPSPLENMSFEDFDCDLESLVHSISIDSLL

Target ORF information:

RefSeq Version	XM_017597035.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597035.1

ORF Insert Sequence:

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGCAG 
GGTGGCAACA TCCAGGGTCA CTGGGCTCGC TGGAAGAGCC TCTGGTGTGG TATGGGGACC 
ATCAGGTCAG GTCTGGAAGA ACTGTGGGAA CTACAGAGGC ATCAGTGCCT ACATCAGGAG 
CTCCTCCAGC CAGCCCCATT GCTGCTAGAG AAGCCTCTGT CTGAATGGCC AGTGCCTCAG 
TTCATCAGCC TCTTTCTACC AGAGTTTCCC ATGCGGCCTG TTAAAGAGCA GGAGCTGAAG 
ATTTTAGGCC TTGTGGCTAA AGGTTCGTTT GGAACTGTCC TGAAAGTGCT AGATTGTGCC 
CAGAATGCTG TATTTGCAGT GAAGGTGGTA CCCAAAGTAA AAGTTCTACA GCGGGATACC 
CTGAGGCAGT GCAAGGAGGA GGTTAGCATC CAGCGGCAGA TCAACCATCC TTTTGTACAC 
AGTTTGGGGG ACAGCTGGCA GGGGAAACGA CACCTCTTTA TTATGTGTAG CTACTGCAGC 
ATGGATCTCT ACTCCCTGTG GTCCACTGTT GGTTGTTTTC CAGAGGATTC TGTCCGTCTC 
TTTGCAGCTG AACTGGTCCT TGTACTCTGC TATCTCCATG ACTTGGGCAT CATCCATCGT 
GATGTAAAGA TGGAGAATAT CCTGCTGGAT GAACGAGGCC ATCTGAAAGT TACAGACTTT 
GGTCTGTCTC GCCGCCTATC CCAGGGAGCA CGGGCCTATA CTATCTGTGG CACTCTTCAA 
TACATGGCTC CAGAGGTCTT GAGTGGTGGG CCTTACAACC ACACTGCTGA CTGGTGGTCC 
CTGGGAGTAT TGCTTTTCTC TCTGGCAGCT GGTAAGTTCC CAGTGGCTGC AGAGAGAGAT 
CATTTGACCA TGTTGGCAAG TGTGACCCAC TGTGGATCTG AGATCCCAGC GTCTATTAAT 
CAGGAGCTCT CACTCCTGCT CCATGAGCTC TTATGCCAGA ACCCTCTGTA CCGTCTACGA 
TACCTGCATC ACTTCCAGGC CCACCCTTTC TTCCGGGGTG TGGCCTTTGA CCCTGAGCTC 
CTACAGAAGC AGCCAGTGAA CTTTGTCATG GAGACACAAG ATACCTGGCC CAGTCCATTG 
GAGAACATGT CCTTTGAGGA CTTCGACTGT GATCTCGAGT CCTTGGTCCA TTCCATCTCT 
ATTGACTCTC TACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_017604093.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017459582.1 Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

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CloneID	ORa53954
Clone ID Related Accession (Same CDS sequence)	XM_017597035.1 , XM_017604093.1
Accession Version	XM_017604093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGCAG 
GGTGGCAACA TCCAGGGTCA CTGGGCTCGC TGGAAGAGCC TCTGGTGTGG TATGGGGACC 
ATCAGGTCAG GTCTGGAAGA ACTGTGGGAA CTACAGAGGC ATCAGTGCCT ACATCAGGAG 
CTCCTCCAGC CAGCCCCATT GCTGCTAGAG AAGCCTCTGT CTGAATGGCC AGTGCCTCAG 
TTCATCAGCC TCTTTCTACC AGAGTTTCCC ATGCGGCCTG TTAAAGAGCA GGAGCTGAAG 
ATTTTAGGCC TTGTGGCTAA AGGTTCGTTT GGAACTGTCC TGAAAGTGCT AGATTGTGCC 
CAGAATGCTG TATTTGCAGT GAAGGTGGTA CCCAAAGTAA AAGTTCTACA GCGGGATACC 
CTGAGGCAGT GCAAGGAGGA GGTTAGCATC CAGCGGCAGA TCAACCATCC TTTTGTACAC 
AGTTTGGGGG ACAGCTGGCA GGGGAAACGA CACCTCTTTA TTATGTGTAG CTACTGCAGC 
ATGGATCTCT ACTCCCTGTG GTCCACTGTT GGTTGTTTTC CAGAGGATTC TGTCCGTCTC 
TTTGCAGCTG AACTGGTCCT TGTACTCTGC TATCTCCATG ACTTGGGCAT CATCCATCGT 
GATGTAAAGA TGGAGAATAT CCTGCTGGAT GAACGAGGCC ATCTGAAAGT TACAGACTTT 
GGTCTGTCTC GCCGCCTATC CCAGGGAGCA CGGGCCTATA CTATCTGTGG CACTCTTCAA 
TACATGGCTC CAGAGGTCTT GAGTGGTGGG CCTTACAACC ACACTGCTGA CTGGTGGTCC 
CTGGGAGTAT TGCTTTTCTC TCTGGCAGCT GGTAAGTTCC CAGTGGCTGC AGAGAGAGAT 
CATTTGACCA TGTTGGCAAG TGTGACCCAC TGTGGATCTG AGATCCCAGC GTCTATTAAT 
CAGGAGCTCT CACTCCTGCT CCATGAGCTC TTATGCCAGA ACCCTCTGTA CCGTCTACGA 
TACCTGCATC ACTTCCAGGC CCACCCTTTC TTCCGGGGTG TGGCCTTTGA CCCTGAGCTC 
CTACAGAAGC AGCCAGTGAA CTTTGTCATG GAGACACAAG ATACCTGGCC CAGTCCATTG 
GAGAACATGT CCTTTGAGGA CTTCGACTGT GATCTCGAGT CCTTGGTCCA TTCCATCTCT 
ATTGACTCTC TACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017459582.1
CDS	118..1335
Translation	MGAVSCRQGQHTRVSAPQKQGGNIQGHWARWKSLWCGMGTIRSGLEELWELQRHQCLHQELLQPAPLLLEKPLSEWPVPQFISLFLPEFPMRPVKEQELKILGLVAKGSFGTVLKVLDCAQNAVFAVKVVPKVKVLQRDTLRQCKEEVSIQRQINHPFVHSLGDSWQGKRHLFIMCSYCSMDLYSLWSTVGCFPEDSVRLFAAELVLVLCYLHDLGIIHRDVKMENILLDERGHLKVTDFGLSRRLSQGARAYTICGTLQYMAPEVLSGGPYNHTADWWSLGVLLFSLAAGKFPVAAERDHLTMLASVTHCGSEIPASINQELSLLLHELLCQNPLYRLRYLHHFQAHPFFRGVAFDPELLQKQPVNFVMETQDTWPSPLENMSFEDFDCDLESLVHSISIDSLL

Target ORF information:

RefSeq Version	XM_017604093.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017604093.1

ORF Insert Sequence:

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGCAG 
GGTGGCAACA TCCAGGGTCA CTGGGCTCGC TGGAAGAGCC TCTGGTGTGG TATGGGGACC 
ATCAGGTCAG GTCTGGAAGA ACTGTGGGAA CTACAGAGGC ATCAGTGCCT ACATCAGGAG 
CTCCTCCAGC CAGCCCCATT GCTGCTAGAG AAGCCTCTGT CTGAATGGCC AGTGCCTCAG 
TTCATCAGCC TCTTTCTACC AGAGTTTCCC ATGCGGCCTG TTAAAGAGCA GGAGCTGAAG 
ATTTTAGGCC TTGTGGCTAA AGGTTCGTTT GGAACTGTCC TGAAAGTGCT AGATTGTGCC 
CAGAATGCTG TATTTGCAGT GAAGGTGGTA CCCAAAGTAA AAGTTCTACA GCGGGATACC 
CTGAGGCAGT GCAAGGAGGA GGTTAGCATC CAGCGGCAGA TCAACCATCC TTTTGTACAC 
AGTTTGGGGG ACAGCTGGCA GGGGAAACGA CACCTCTTTA TTATGTGTAG CTACTGCAGC 
ATGGATCTCT ACTCCCTGTG GTCCACTGTT GGTTGTTTTC CAGAGGATTC TGTCCGTCTC 
TTTGCAGCTG AACTGGTCCT TGTACTCTGC TATCTCCATG ACTTGGGCAT CATCCATCGT 
GATGTAAAGA TGGAGAATAT CCTGCTGGAT GAACGAGGCC ATCTGAAAGT TACAGACTTT 
GGTCTGTCTC GCCGCCTATC CCAGGGAGCA CGGGCCTATA CTATCTGTGG CACTCTTCAA 
TACATGGCTC CAGAGGTCTT GAGTGGTGGG CCTTACAACC ACACTGCTGA CTGGTGGTCC 
CTGGGAGTAT TGCTTTTCTC TCTGGCAGCT GGTAAGTTCC CAGTGGCTGC AGAGAGAGAT 
CATTTGACCA TGTTGGCAAG TGTGACCCAC TGTGGATCTG AGATCCCAGC GTCTATTAAT 
CAGGAGCTCT CACTCCTGCT CCATGAGCTC TTATGCCAGA ACCCTCTGTA CCGTCTACGA 
TACCTGCATC ACTTCCAGGC CCACCCTTTC TTCCGGGGTG TGGCCTTTGA CCCTGAGCTC 
CTACAGAAGC AGCCAGTGAA CTTTGTCATG GAGACACAAG ATACCTGGCC CAGTCCATTG 
GAGAACATGT CCTTTGAGGA CTTCGACTGT GATCTCGAGT CCTTGGTCCA TTCCATCTCT 
ATTGACTCTC TACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( NM_001044224.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001037689.1 Rattus norvegicus sperm associated antigen 5 (Spag5), mRNA.

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CloneID	ORa09938
Clone ID Related Accession (Same CDS sequence)	NM_001044224.1
Accession Version	NM_001044224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB231661.1. On or before Aug 24, 2006 this sequence version replaced XM_340848.3, XM_001080820.1. ##Evidence-Data-START## Transcript exon combination :: AB231661.1, BC166408.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGAGGG TGAAAACACT GAATCTCGGC TTGTCGCCTT CGCCCCAATC GGGGAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC TGATTCAGGG 
AAAGGTCCTA TGTTCTCCTC GCTGACCCCA TACCTGCGCA GGCTGGATCT GAAGGAAAGG 
CGCAACAGCT CATCTCCAGT GAATTTTGTT AATGCAGAGA ACTCATCTCC AGTGGATTTT 
GTCAATACTG AGAACACATC TCCAGTGGAT TTTGACAATA CTGAGAACAA CTTCCTTTCA 
GAACAGTTCA GCCATCCTTC AGAGTACATA GAAGCTTGTC AGCGTGAATC AGATCTAACT 
CCTGAAAGCA GCTCTCCTTT CAACACACTG GAGAAGGCAG TGGAAGCAAT GGATGATTTT 
GTGGCAGAAC CAATAGATGA CGGTATAGTT AAGACCATGG TTCTTTTACC CTTTTCCTTA 
GGGCAGCAGC AAGACCTGAT GCTCCAGGCT CACTTAGATA CCACAGCAGA GAGAAATAAA 
AGCTCTCTAA AAGAATCTTT GGGGCTAGAA GACCTGGTGG AAAAGGAGGC GGCACCTTGT 
GTGGAAGACA GCTTACCAGA AACTGTTGCT ATCAGGCCTG AGCAACCCAT CTCTCAGGAC 
CCTCCATTAA GAGACAGTAA CACTGAAGCT GCACCTGCGG GCTCAGTTCC TTCTGAAAAT 
GTCTTGGGTT TCACTGTGGC TTGTCTCTCT CCCTCAACTG TCTTGACCAA AGATTTCTCT 
GTCGATCATG TCGATCCTGG GGAGGAAACT GCAGAGAACA GAGTTATACA GGAAGTGGAA 
AAGAGTTTTC TCGCACTCCC TGAGGAGGCT GGATTGGGAG ATCAGGCACT TGCTGTAAAT 
GCAGAAGACG TCTCATCCTT ATACCTGTCA TCAAGTCTAG TAGAAATGGG ACCCCAGGAG 
GCTCCAGGCC CAACAGCAGA AGATGCCAGT AGGATTCCTG ACTTGGAGTC AGAGACTTGG 
ATGTCCCCAT TGGCCTGGCT AGAAAAAGGT GTGAACACGT CAGTCATGCT ACAAAGTCTC 
CGCCAGAGTT TACCCTTTTC AAAATCTGTG CTTCAGGATG CTGCCGTTGG CAATACACCC 
CACTCCACTT ATTCTGTGGG AACTTCCTTT ACTCCTCCAG TGCCACTGGA AGTAGGCACC 
AAGGACAGTA CTTCAGAGAC AGAGCGCCTC CTCTTGGGCT GCCGTCCTCC AGATCTGGCA 
GCCTTGTCCC GGCACGACTT GGAAGAGAAC CTGTTGAACG CCCTTGTCTT GCTGGAAGTT 
CTTTCTCACC AGCTGCAAGC CTGGAAGAGT CAGCTGACTG TCCCGAGCCG GCAAGCTCAA 
GACAGCAGCA CACAGACCGA CAGCTCTGCA GCTGTGGTCA CTAAGACACC TAAACATCTT 
CAGGACAGCA AGGAGATTCG ACAGGCTCTA CTGCAAGCCA GGAATGTCAT ACATTCATGG 
GGGCTAGTCT CTAGAGACCT GATGTCCTTG CTTCACCTGT CCCTGACACA TGTGCAAGAT 
GACAGAGTGA CAGTGAGTCA GGAGTCTCAG CGTGCAGAGA CCCTGGTTTC CTCCTGTTCT 
CGTGTACTGA AGAAATTGAG GGCAAAGCTC CAGAGCCTCA AAACAGAATG GGAGGAGGCA 
CGGCACAGGG AGGAAATGGC CCTTAAAGGC AAGGATGCGG CAGAGGCAGT ACTGGAGGCT 
TTCCGTGCAC ACGCTAGCCA GCGCATCAGC CAACTGGAAC AGGGTATCAC ATCTGTGCAG 
GAATTCAGAG GCCTTCTACA GGAGGCACAG ACCCAACTGA TAGGGCTTCA CACAGAGCAA 
GAAGAGCTGG CTCAGCACAC AGTGAGTCTG ACTTCTGCCT TGCAGCAGGA CTGGACATCA 
GTCCAACAGA ATTATGGGAC ATGGGCAGCT TTGCTGAGTC GGTCTCGAGA ACTCACCAAG 
AAACTCACAG CCAAGAGCCG GCAAGCCCTT CAGGAACGTG ATGCTGCAAT TGAGGAAAAG 
GATCAGGTTG TGAAGGAAGT GGAACAAGTC TCTGCCCATT TAGAGGACTG TAAAGGCCAA 
ATAGAACAAC TGAAGTTGGA AAATAGTCGC CTTACTGCAG ATCTCTCGGC TCAGCTGCAG 
ACTCTGGCTA GCACAGAGAA CGAACTAAAG GAACTACAGA GTCAGCATTC CCATTGTGTC 
CAAGACTTGG CCATGAAGGA TGAACTGCTG TGTCAACTTA CCCAGAGCAA CAAGGAACAG 
GCTGCTCAGT GGCAAAAGGA AGAAACGGAA CTGAAACACA GACAAGCAGA ACTGCTGCAG 
CAGAAGGCTG TGTTGGCTAA GGAGGTACAG GACCTGAGGG AGACCATGGA GTTTGTAGAT 
GAAGAGAGTC AAGTTGCTCA CCTGGAGCTG GGCCAGATCG AGAGTCAGTT GAAAGCCACA 
CTAGAAGTTC TGCGGGAGCG CAGCCTGCAG TGTGAGACCC TCAAGGACAC CGTGGAGAGC 
CTGAGGGCAG AGCTGGCCAG CACCGAAGCA AAGCATGAGC AACAAGCGCT GGAGAAGACG 
CACCAGCATT CTAAAGAGCT GTGTCTATTG GCGGAGCAGC TACAGAGTCT CACCCTCTTC 
TTAGAGGCTA AACTAGAGGA GAACAAGGCA GAATCAGACA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCCTTCC AGTGATAGCA GCGTCTCAGA ACAGATCCCG 
ACAGCAGTGG TAGATGAAGT GCCAGAACCA GCTCCTGTGC CATTGCTTGG AAGTGTTAAG 
AGTGCTTTCA CCCGCGTAGC CTCAACAGCT CCCTTTCGGC CTACAGAGAC CCCAGCCCTG 
GAGAAGAGCC TGGCAGAAAT GAGTGCTGTG TTACAGGAGC TTCAACGCCT CTGTGCCCTG 
CTGCAAGAAT CTAAAGAGGA AGCCGTTGGT GTCCTGCAGA GGGAAATCTG TGAACTGCAC 
ACAAGACTAC AGGCCCAAGA AGAAGAACAT CAGGAAGCCC AAAAGGCAAA GGAAGCAGAC 
ATAGAGAAGT TGAACCAGGC CTTGTGCTTG CGCCACAAGA ATGAGAAGGA ACTCCTGGAA 
GTGATACAGA AGCAGAACGA AAAGATCCTG GGGCAGATAG ACACGAGTGG CCAGCTCATA 
AACCTCAGGG AGGAGGTGAC CCAGCTTACA CGTTTACTTC GGCGGGCAGA GACAGAGACT 
AAAGTGCTCC AGGAAGCCCT GGAAGGCCAG GTAGATCCCA GCTGTCAGCT GATGGCTACT 
AACTGGATCC AGGAAAAAGT GTTTCTCTCG CAGGAGGTGA ACAAACTGAG GGCTATGTTC 
CTGGAGGTGA AAAATGAGAA GAAACAGCTG ATGGACAAGT ATCTGAGCCA TAGGCACATC 
CTGGAGGAGA ATCTTCGGCG CTCTGACACA GAGTTAAAGA AACTTGATGA CACAATTCAG 
CATATCTATG AGACTCTGTT GTCTATCCCA GAGGTAGTGA AGAGTTGCAA GGAGCTACAA 
GGATTGCTAG AATTTCTGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037689.1
CDS	59..3622
Translation	MWRVKTLNLGLSPSPQSGKPAMRTPLRELKLQPEALADSGKGPMFSSLTPYLRRLDLKERRNSSSPVNFVNAENSSPVDFVNTENTSPVDFDNTENNFLSEQFSHPSEYIEACQRESDLTPESSSPFNTLEKAVEAMDDFVAEPIDDGIVKTMVLLPFSLGQQQDLMLQAHLDTTAERNKSSLKESLGLEDLVEKEAAPCVEDSLPETVAIRPEQPISQDPPLRDSNTEAAPAGSVPSENVLGFTVACLSPSTVLTKDFSVDHVDPGEETAENRVIQEVEKSFLALPEEAGLGDQALAVNAEDVSSLYLSSSLVEMGPQEAPGPTAEDASRIPDLESETWMSPLAWLEKGVNTSVMLQSLRQSLPFSKSVLQDAAVGNTPHSTYSVGTSFTPPVPLEVGTKDSTSETERLLLGCRPPDLAALSRHDLEENLLNALVLLEVLSHQLQAWKSQLTVPSRQAQDSSTQTDSSAAVVTKTPKHLQDSKEIRQALLQARNVIHSWGLVSRDLMSLLHLSLTHVQDDRVTVSQESQRAETLVSSCSRVLKKLRAKLQSLKTEWEEARHREEMALKGKDAAEAVLEAFRAHASQRISQLEQGITSVQEFRGLLQEAQTQLIGLHTEQEELAQHTVSLTSALQQDWTSVQQNYGTWAALLSRSRELTKKLTAKSRQALQERDAAIEEKDQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQTLASTENELKELQSQHSHCVQDLAMKDELLCQLTQSNKEQAAQWQKEETELKHRQAELLQQKAVLAKEVQDLRETMEFVDEESQVAHLELGQIESQLKATLEVLRERSLQCETLKDTVESLRAELASTEAKHEQQALEKTHQHSKELCLLAEQLQSLTLFLEAKLEENKAESDIILPSTGCAPAQEHPPSSDSSVSEQIPTAVVDEVPEPAPVPLLGSVKSAFTRVASTAPFRPTETPALEKSLAEMSAVLQELQRLCALLQESKEEAVGVLQREICELHTRLQAQEEEHQEAQKAKEADIEKLNQALCLRHKNEKELLEVIQKQNEKILGQIDTSGQLINLREEVTQLTRLLRRAETETKVLQEALEGQVDPSCQLMATNWIQEKVFLSQEVNKLRAMFLEVKNEKKQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHIYETLLSIPEVVKSCKELQGLLEFLS

Target ORF information:

RefSeq Version	NM_001044224.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044224.1

ORF Insert Sequence:

ATGTGGAGGG TGAAAACACT GAATCTCGGC TTGTCGCCTT CGCCCCAATC GGGGAAACCA 
GCTATGAGAA CTCCTCTCCG AGAGCTTAAA CTACAGCCCG AGGCCCTCGC TGATTCAGGG 
AAAGGTCCTA TGTTCTCCTC GCTGACCCCA TACCTGCGCA GGCTGGATCT GAAGGAAAGG 
CGCAACAGCT CATCTCCAGT GAATTTTGTT AATGCAGAGA ACTCATCTCC AGTGGATTTT 
GTCAATACTG AGAACACATC TCCAGTGGAT TTTGACAATA CTGAGAACAA CTTCCTTTCA 
GAACAGTTCA GCCATCCTTC AGAGTACATA GAAGCTTGTC AGCGTGAATC AGATCTAACT 
CCTGAAAGCA GCTCTCCTTT CAACACACTG GAGAAGGCAG TGGAAGCAAT GGATGATTTT 
GTGGCAGAAC CAATAGATGA CGGTATAGTT AAGACCATGG TTCTTTTACC CTTTTCCTTA 
GGGCAGCAGC AAGACCTGAT GCTCCAGGCT CACTTAGATA CCACAGCAGA GAGAAATAAA 
AGCTCTCTAA AAGAATCTTT GGGGCTAGAA GACCTGGTGG AAAAGGAGGC GGCACCTTGT 
GTGGAAGACA GCTTACCAGA AACTGTTGCT ATCAGGCCTG AGCAACCCAT CTCTCAGGAC 
CCTCCATTAA GAGACAGTAA CACTGAAGCT GCACCTGCGG GCTCAGTTCC TTCTGAAAAT 
GTCTTGGGTT TCACTGTGGC TTGTCTCTCT CCCTCAACTG TCTTGACCAA AGATTTCTCT 
GTCGATCATG TCGATCCTGG GGAGGAAACT GCAGAGAACA GAGTTATACA GGAAGTGGAA 
AAGAGTTTTC TCGCACTCCC TGAGGAGGCT GGATTGGGAG ATCAGGCACT TGCTGTAAAT 
GCAGAAGACG TCTCATCCTT ATACCTGTCA TCAAGTCTAG TAGAAATGGG ACCCCAGGAG 
GCTCCAGGCC CAACAGCAGA AGATGCCAGT AGGATTCCTG ACTTGGAGTC AGAGACTTGG 
ATGTCCCCAT TGGCCTGGCT AGAAAAAGGT GTGAACACGT CAGTCATGCT ACAAAGTCTC 
CGCCAGAGTT TACCCTTTTC AAAATCTGTG CTTCAGGATG CTGCCGTTGG CAATACACCC 
CACTCCACTT ATTCTGTGGG AACTTCCTTT ACTCCTCCAG TGCCACTGGA AGTAGGCACC 
AAGGACAGTA CTTCAGAGAC AGAGCGCCTC CTCTTGGGCT GCCGTCCTCC AGATCTGGCA 
GCCTTGTCCC GGCACGACTT GGAAGAGAAC CTGTTGAACG CCCTTGTCTT GCTGGAAGTT 
CTTTCTCACC AGCTGCAAGC CTGGAAGAGT CAGCTGACTG TCCCGAGCCG GCAAGCTCAA 
GACAGCAGCA CACAGACCGA CAGCTCTGCA GCTGTGGTCA CTAAGACACC TAAACATCTT 
CAGGACAGCA AGGAGATTCG ACAGGCTCTA CTGCAAGCCA GGAATGTCAT ACATTCATGG 
GGGCTAGTCT CTAGAGACCT GATGTCCTTG CTTCACCTGT CCCTGACACA TGTGCAAGAT 
GACAGAGTGA CAGTGAGTCA GGAGTCTCAG CGTGCAGAGA CCCTGGTTTC CTCCTGTTCT 
CGTGTACTGA AGAAATTGAG GGCAAAGCTC CAGAGCCTCA AAACAGAATG GGAGGAGGCA 
CGGCACAGGG AGGAAATGGC CCTTAAAGGC AAGGATGCGG CAGAGGCAGT ACTGGAGGCT 
TTCCGTGCAC ACGCTAGCCA GCGCATCAGC CAACTGGAAC AGGGTATCAC ATCTGTGCAG 
GAATTCAGAG GCCTTCTACA GGAGGCACAG ACCCAACTGA TAGGGCTTCA CACAGAGCAA 
GAAGAGCTGG CTCAGCACAC AGTGAGTCTG ACTTCTGCCT TGCAGCAGGA CTGGACATCA 
GTCCAACAGA ATTATGGGAC ATGGGCAGCT TTGCTGAGTC GGTCTCGAGA ACTCACCAAG 
AAACTCACAG CCAAGAGCCG GCAAGCCCTT CAGGAACGTG ATGCTGCAAT TGAGGAAAAG 
GATCAGGTTG TGAAGGAAGT GGAACAAGTC TCTGCCCATT TAGAGGACTG TAAAGGCCAA 
ATAGAACAAC TGAAGTTGGA AAATAGTCGC CTTACTGCAG ATCTCTCGGC TCAGCTGCAG 
ACTCTGGCTA GCACAGAGAA CGAACTAAAG GAACTACAGA GTCAGCATTC CCATTGTGTC 
CAAGACTTGG CCATGAAGGA TGAACTGCTG TGTCAACTTA CCCAGAGCAA CAAGGAACAG 
GCTGCTCAGT GGCAAAAGGA AGAAACGGAA CTGAAACACA GACAAGCAGA ACTGCTGCAG 
CAGAAGGCTG TGTTGGCTAA GGAGGTACAG GACCTGAGGG AGACCATGGA GTTTGTAGAT 
GAAGAGAGTC AAGTTGCTCA CCTGGAGCTG GGCCAGATCG AGAGTCAGTT GAAAGCCACA 
CTAGAAGTTC TGCGGGAGCG CAGCCTGCAG TGTGAGACCC TCAAGGACAC CGTGGAGAGC 
CTGAGGGCAG AGCTGGCCAG CACCGAAGCA AAGCATGAGC AACAAGCGCT GGAGAAGACG 
CACCAGCATT CTAAAGAGCT GTGTCTATTG GCGGAGCAGC TACAGAGTCT CACCCTCTTC 
TTAGAGGCTA AACTAGAGGA GAACAAGGCA GAATCAGACA TCATTCTGCC CAGCACAGGC 
TGTGCTCCAG CCCAGGAACA CCCTCCTTCC AGTGATAGCA GCGTCTCAGA ACAGATCCCG 
ACAGCAGTGG TAGATGAAGT GCCAGAACCA GCTCCTGTGC CATTGCTTGG AAGTGTTAAG 
AGTGCTTTCA CCCGCGTAGC CTCAACAGCT CCCTTTCGGC CTACAGAGAC CCCAGCCCTG 
GAGAAGAGCC TGGCAGAAAT GAGTGCTGTG TTACAGGAGC TTCAACGCCT CTGTGCCCTG 
CTGCAAGAAT CTAAAGAGGA AGCCGTTGGT GTCCTGCAGA GGGAAATCTG TGAACTGCAC 
ACAAGACTAC AGGCCCAAGA AGAAGAACAT CAGGAAGCCC AAAAGGCAAA GGAAGCAGAC 
ATAGAGAAGT TGAACCAGGC CTTGTGCTTG CGCCACAAGA ATGAGAAGGA ACTCCTGGAA 
GTGATACAGA AGCAGAACGA AAAGATCCTG GGGCAGATAG ACACGAGTGG CCAGCTCATA 
AACCTCAGGG AGGAGGTGAC CCAGCTTACA CGTTTACTTC GGCGGGCAGA GACAGAGACT 
AAAGTGCTCC AGGAAGCCCT GGAAGGCCAG GTAGATCCCA GCTGTCAGCT GATGGCTACT 
AACTGGATCC AGGAAAAAGT GTTTCTCTCG CAGGAGGTGA ACAAACTGAG GGCTATGTTC 
CTGGAGGTGA AAAATGAGAA GAAACAGCTG ATGGACAAGT ATCTGAGCCA TAGGCACATC 
CTGGAGGAGA ATCTTCGGCG CTCTGACACA GAGTTAAAGA AACTTGATGA CACAATTCAG 
CATATCTATG AGACTCTGTT GTCTATCCCA GAGGTAGTGA AGAGTTGCAA GGAGCTACAA 
GGATTGCTAG AATTTCTGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag5 ( XM_017597034.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452523.1 Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57195
Clone ID Related Accession (Same CDS sequence)	XM_017597034.1
Accession Version	XM_017597034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sperm-associated antigen 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGG 
AAACCAGCTA TGAGAACTCC TCTCCGAGAG CTTAAACTAC AGCCCGAGGC CCTCGCTGAT 
TCAGGGAAAG GTCCTATGTT CTCCTCGCTG ACCCCATACC TGCGCAGGCT GGATCTGAAG 
GAAAGGCGCA ACAGCTCATC TCCAGTGAAT TTTGTTAATG NNGCAGAGAA CTCATCTCCA 
GTGGATTTTG TCAATACTGA GAACACATCT CCAGTGGATT TTGACAATAC TGAGAACAAC 
TTCCTTTCAG AACAGTTCAG CCATCCTTCA GAGTACATAG AAGCTTGTCA GCGTGAATCA 
GATCTAACTC CTGAAAGCAG CTCTCCTTTC AACACACTGG AGAAGGCAGT GGAAGCAATG 
GATGATTTTG TGGCAGAACC AATAGATGAC GGTATAGTTA AGACCATGGT TCTTTTACCC 
TTTTCCTTAG GGCAGCAGCA AGACCTGATG CTCCAGGCTC ACTTAGATAC CACAGCAGAG 
AGAAATAAAA GCTCTCTAAA AGAATCTTTG GGGCTAGAAG ACCTGGTGGA AAAGGAGGCG 
GCACCTTGTG TGGAAGACAG CTTACCAGAA ACTGTTGCTA TCAGGCCTGA GCAACCCATC 
TCTCAGGACC CTCCATTAAG AGACAGTAAC ACTGAAGCTG CACCTGCGGG CTCAGTTCCT 
TCTGAAAATG TCTTGGATTT CACTGTGGCT TGTCTCTCTC CCTCAACTGT CTTGACCAAA 
GATTTCTCTG TCGATCATGT CGATCCTGGG GAGGAAACTG CAGAGAACAG AGTTATACAG 
GAAGTGGAAA AGAGTTTTCT CGCACTCCCT GAGGAGGCTG GATTGGGAGA TCAGGCACTT 
GCTGTAAATG CAGAAGACGT CTCATCCTTA TACCTGTCAT CAAGTCTAGT AGAAATGGGA 
CCCCAGGAGG CTCCAGGCCC AACAGCAGAA GATGCCAGTA GGATTCCTGA CTTGGAGTCA 
GAGACTTGGA TGTCCCCATT GGCCTGGCTA GAAAAAGGTG TGAACACGTC AGTCATGCTA 
CAAAGTCTCC GCCAGAGTTT ACCCTTTTCA AAATCTGTGC TTCAGGATGC TGCCGTTGGC 
AATACACCCC ACTCCACTTA TTCTGTGGGA ACTTCCTTTA CTCCTCCAGT GCCACTGGAA 
GTAGGCACCA AGGACAGTAC TTCAGAGACA GAGCGCCTCC TCTTGGGCTG CCGTCCTCCA 
GATCTGGCAG CCTTGTCCCG GCACGACTTG GAAGAGAACC TGTTGAACGC CCTTGTCTTG 
CTGGAAGTTC TTTCTCACCA GCTGCAAGCC TGGAAGAGTC AGCTGACTGT CCCGAGCCGG 
CAAGCTCAAG ACAGCAGCAC ACAGACCGAC AGCTCTGCAG CTGTGGTCAC TAAGACACCT 
AAACATCTTC AGGACAGCAA GGAGATTCGA CAGGCTCTAC TGCAAGCCAG GAATGTCATA 
CATTCATGGG GGCTAGTCTC TAGAGACCTG ATGTCCTTGC TTCACCTGTC CCTGACACAT 
GTGCAAGATG ACAGAGTGAC AGTGAGTCAG GAGTCTCAGC GTGCAGAGAC CCTGGTTTCC 
TCCTGTTCTC GTGTACTGAA GAAATTGAGG GCAAAGCTCC AGAGCCTCAA AACAGAATGG 
GAGGAGGCAC GGCACAGGGA GGAAATGGCC CTTAAAGGCA AGGATGCGGC AGAGGCAGTA 
CTGGAGGCTT TCCGTGCACA CGCTAGCCAG CGCATCAGCC AACTGGAACA GGGTATCACA 
TCTGTGCAGG AATTCAGAGG CCTTCTACAG GAGGCACAGA CCCAACTGAT AGGGCTTCAC 
ACAGAGCAAG AAGAGCTGGC TCAGCACACA GTGAGTCTGA CTTCTGCCTT GCAGCAGGAC 
TGGACATCAG TCCAACAGAA TTATGGGACA TGGGCAGCTT TGCTGAGTCG GTCTCGAGAA 
CTCACCAAGA AACTCACAGC CAAGAGCCGG CAAGCCCTTC AGGAACGTGA TGCTGCAATT 
GAGGAAAAGG ATCAGGTTGT GAAGGAAGTG GAACAAGTCT CTGCCCATTT AGAGGACTGT 
AAAGGCCAAA TAGAACAACT GAAGTTGGAA AATAGTCGCC TTACTGCAGA TCTCTCGGCT 
CAGCTGCAGA CTCTGGCTAG CACAGAGAAC GAACTAAAGG AACTACAGAG TCAGCATTCC 
CATTGTGTCC AAGACTTGGC CATGAAGGAT GAACTGCTGT GTCAACTTAC CCAGAGCAAC 
AAGGAACAGG CTGCTCAGTG GCAAAAGGAA GAAACGGAAC TGAAACACAG ACAAGCAGAA 
CTGCTGCAGC AGAAGGCTGT GTTGGCTAAG GAGGTACAGG ACCTGAGGGA GACCATGGAG 
TTTGTAGATG AAGAGAGTCA AGTTGCTCAC CTGGAGCTGG GCCAGATCGA GAGTCAGTTG 
AAAGCCACAC TAGAAGTTCT GCGGGAGCGC AGCCTGCAGT GTGAGACCCT CAAGGACACC 
GTGGAGAGCC TGAGGGCAGA GCTGGCCAGC ACCGAAGCAA AGCATGAGCA ACAAGCGCTG 
GAGAAGACGC ACCAGCATTC TAAAGAGCTG TGTCTATTGG CGGAGCAGCT ACAGAGTCTC 
ACCCTCTTCT TAGAGGCTAA ACTAGAGGAG AACAAGGCAG AATCAGACAT CATTCTGCCC 
AGCACAGGCT GTGCTCCAGC CCAGGAACAC CCTCCTTCCA GTGATAGCAG CGTCTCAGAA 
CAGATCCCGA CAGCAGTGGT AGATGAAGTG CCAGAACCAG CTCCTGTGCC ATTGCTTGGA 
AGTGTTAAGA GTGCTTTCAC CCGCGTAGCC TCAACAGCTC CCTTTCGGCC TACAGAGACC 
CCAGCCCTGG AGAAGAGCCT GGCAGAAATG AGTGCTGTGT TACAGGAGCT TCAACGCCTC 
TGTGCCCTGC TGCAAGAATC TAAAGAGGAA GCCGTTGGTG TCCTGCAGAG GGAAATCTGT 
GAACTGCACA CAAGACTACA GGCCCAAGAA GAAGAACATC AGGAAGCCCA AAAGGCAAAG 
GAAGCAGACA TAGAGAAGTT GAACCAGGCC TTGTGCTTGC GCCACAAGAA TGAGAAGGAA 
CTCCTGGAAG TGATACAGAA GCAGAACGAA AAGATCCTGG GGCAGATAGA CACGAGTGGC 
CAGCTCATAA ACCTCAGGGA GGAGGTGACC CAGCTTACAC GTTTACTTCG GCGGGCAGAG 
ACAGAGACTA AAGTGCTCCA GGAAGCCCTG GAAGGCCAGG TAGATCCCAG CTGTCAGCTG 
ATGGCTACTA ACTGGATCCA GGAAAAAGTG TTTCTCTCGC AGGAGGTGAA CAAACTGAGG 
GCTATGTTCC TGGAGGTGAA AAATGAGAAG AAACAGCTGA TGGACAAGTA TCTGAGCCAT 
AGGCACATCC TGGAGGAGAA TCTTCGGCGC TCTGACACAG AGTTAAAGAA ACTTGATGAC 
ACAATTCAGC ATATCTATGA GACTCTGTTG TCTATCCCAG AGGTAGTGAA GAGTTGCAAG 
GAGCTACAAG GATTGCTAGA ATTTCTGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452523.1
CDS	118..3690
Translation	MGAVSCRQGQHTRVSAPQKGKPAMRTPLRELKLQPEALADSGKGPMFSSLTPYLRRLDLKERRNSSSPVNFVNnullAENSSPVDFVNTENTSPVDFDNTENNFLSEQFSHPSEYIEACQRESDLTPESSSPFNTLEKAVEAMDDFVAEPIDDGIVKTMVLLPFSLGQQQDLMLQAHLDTTAERNKSSLKESLGLEDLVEKEAAPCVEDSLPETVAIRPEQPISQDPPLRDSNTEAAPAGSVPSENVLDFTVACLSPSTVLTKDFSVDHVDPGEETAENRVIQEVEKSFLALPEEAGLGDQALAVNAEDVSSLYLSSSLVEMGPQEAPGPTAEDASRIPDLESETWMSPLAWLEKGVNTSVMLQSLRQSLPFSKSVLQDAAVGNTPHSTYSVGTSFTPPVPLEVGTKDSTSETERLLLGCRPPDLAALSRHDLEENLLNALVLLEVLSHQLQAWKSQLTVPSRQAQDSSTQTDSSAAVVTKTPKHLQDSKEIRQALLQARNVIHSWGLVSRDLMSLLHLSLTHVQDDRVTVSQESQRAETLVSSCSRVLKKLRAKLQSLKTEWEEARHREEMALKGKDAAEAVLEAFRAHASQRISQLEQGITSVQEFRGLLQEAQTQLIGLHTEQEELAQHTVSLTSALQQDWTSVQQNYGTWAALLSRSRELTKKLTAKSRQALQERDAAIEEKDQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQTLASTENELKELQSQHSHCVQDLAMKDELLCQLTQSNKEQAAQWQKEETELKHRQAELLQQKAVLAKEVQDLRETMEFVDEESQVAHLELGQIESQLKATLEVLRERSLQCETLKDTVESLRAELASTEAKHEQQALEKTHQHSKELCLLAEQLQSLTLFLEAKLEENKAESDIILPSTGCAPAQEHPPSSDSSVSEQIPTAVVDEVPEPAPVPLLGSVKSAFTRVASTAPFRPTETPALEKSLAEMSAVLQELQRLCALLQESKEEAVGVLQREICELHTRLQAQEEEHQEAQKAKEADIEKLNQALCLRHKNEKELLEVIQKQNEKILGQIDTSGQLINLREEVTQLTRLLRRAETETKVLQEALEGQVDPSCQLMATNWIQEKVFLSQEVNKLRAMFLEVKNEKKQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHIYETLLSIPEVVKSCKELQGLLEFLS

Target ORF information:

RefSeq Version	XM_017597034.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm associated antigen 5 (Spag5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597034.1

ORF Insert Sequence:

ATGGGAGCAG TGAGCTGTCG ACAGGGACAA CACACCCGAG TGTCTGCTCC TCAAAAGGGG 
AAACCAGCTA TGAGAACTCC TCTCCGAGAG CTTAAACTAC AGCCCGAGGC CCTCGCTGAT 
TCAGGGAAAG GTCCTATGTT CTCCTCGCTG ACCCCATACC TGCGCAGGCT GGATCTGAAG 
GAAAGGCGCA ACAGCTCATC TCCAGTGAAT TTTGTTAATG NNGCAGAGAA CTCATCTCCA 
GTGGATTTTG TCAATACTGA GAACACATCT CCAGTGGATT TTGACAATAC TGAGAACAAC 
TTCCTTTCAG AACAGTTCAG CCATCCTTCA GAGTACATAG AAGCTTGTCA GCGTGAATCA 
GATCTAACTC CTGAAAGCAG CTCTCCTTTC AACACACTGG AGAAGGCAGT GGAAGCAATG 
GATGATTTTG TGGCAGAACC AATAGATGAC GGTATAGTTA AGACCATGGT TCTTTTACCC 
TTTTCCTTAG GGCAGCAGCA AGACCTGATG CTCCAGGCTC ACTTAGATAC CACAGCAGAG 
AGAAATAAAA GCTCTCTAAA AGAATCTTTG GGGCTAGAAG ACCTGGTGGA AAAGGAGGCG 
GCACCTTGTG TGGAAGACAG CTTACCAGAA ACTGTTGCTA TCAGGCCTGA GCAACCCATC 
TCTCAGGACC CTCCATTAAG AGACAGTAAC ACTGAAGCTG CACCTGCGGG CTCAGTTCCT 
TCTGAAAATG TCTTGGATTT CACTGTGGCT TGTCTCTCTC CCTCAACTGT CTTGACCAAA 
GATTTCTCTG TCGATCATGT CGATCCTGGG GAGGAAACTG CAGAGAACAG AGTTATACAG 
GAAGTGGAAA AGAGTTTTCT CGCACTCCCT GAGGAGGCTG GATTGGGAGA TCAGGCACTT 
GCTGTAAATG CAGAAGACGT CTCATCCTTA TACCTGTCAT CAAGTCTAGT AGAAATGGGA 
CCCCAGGAGG CTCCAGGCCC AACAGCAGAA GATGCCAGTA GGATTCCTGA CTTGGAGTCA 
GAGACTTGGA TGTCCCCATT GGCCTGGCTA GAAAAAGGTG TGAACACGTC AGTCATGCTA 
CAAAGTCTCC GCCAGAGTTT ACCCTTTTCA AAATCTGTGC TTCAGGATGC TGCCGTTGGC 
AATACACCCC ACTCCACTTA TTCTGTGGGA ACTTCCTTTA CTCCTCCAGT GCCACTGGAA 
GTAGGCACCA AGGACAGTAC TTCAGAGACA GAGCGCCTCC TCTTGGGCTG CCGTCCTCCA 
GATCTGGCAG CCTTGTCCCG GCACGACTTG GAAGAGAACC TGTTGAACGC CCTTGTCTTG 
CTGGAAGTTC TTTCTCACCA GCTGCAAGCC TGGAAGAGTC AGCTGACTGT CCCGAGCCGG 
CAAGCTCAAG ACAGCAGCAC ACAGACCGAC AGCTCTGCAG CTGTGGTCAC TAAGACACCT 
AAACATCTTC AGGACAGCAA GGAGATTCGA CAGGCTCTAC TGCAAGCCAG GAATGTCATA 
CATTCATGGG GGCTAGTCTC TAGAGACCTG ATGTCCTTGC TTCACCTGTC CCTGACACAT 
GTGCAAGATG ACAGAGTGAC AGTGAGTCAG GAGTCTCAGC GTGCAGAGAC CCTGGTTTCC 
TCCTGTTCTC GTGTACTGAA GAAATTGAGG GCAAAGCTCC AGAGCCTCAA AACAGAATGG 
GAGGAGGCAC GGCACAGGGA GGAAATGGCC CTTAAAGGCA AGGATGCGGC AGAGGCAGTA 
CTGGAGGCTT TCCGTGCACA CGCTAGCCAG CGCATCAGCC AACTGGAACA GGGTATCACA 
TCTGTGCAGG AATTCAGAGG CCTTCTACAG GAGGCACAGA CCCAACTGAT AGGGCTTCAC 
ACAGAGCAAG AAGAGCTGGC TCAGCACACA GTGAGTCTGA CTTCTGCCTT GCAGCAGGAC 
TGGACATCAG TCCAACAGAA TTATGGGACA TGGGCAGCTT TGCTGAGTCG GTCTCGAGAA 
CTCACCAAGA AACTCACAGC CAAGAGCCGG CAAGCCCTTC AGGAACGTGA TGCTGCAATT 
GAGGAAAAGG ATCAGGTTGT GAAGGAAGTG GAACAAGTCT CTGCCCATTT AGAGGACTGT 
AAAGGCCAAA TAGAACAACT GAAGTTGGAA AATAGTCGCC TTACTGCAGA TCTCTCGGCT 
CAGCTGCAGA CTCTGGCTAG CACAGAGAAC GAACTAAAGG AACTACAGAG TCAGCATTCC 
CATTGTGTCC AAGACTTGGC CATGAAGGAT GAACTGCTGT GTCAACTTAC CCAGAGCAAC 
AAGGAACAGG CTGCTCAGTG GCAAAAGGAA GAAACGGAAC TGAAACACAG ACAAGCAGAA 
CTGCTGCAGC AGAAGGCTGT GTTGGCTAAG GAGGTACAGG ACCTGAGGGA GACCATGGAG 
TTTGTAGATG AAGAGAGTCA AGTTGCTCAC CTGGAGCTGG GCCAGATCGA GAGTCAGTTG 
AAAGCCACAC TAGAAGTTCT GCGGGAGCGC AGCCTGCAGT GTGAGACCCT CAAGGACACC 
GTGGAGAGCC TGAGGGCAGA GCTGGCCAGC ACCGAAGCAA AGCATGAGCA ACAAGCGCTG 
GAGAAGACGC ACCAGCATTC TAAAGAGCTG TGTCTATTGG CGGAGCAGCT ACAGAGTCTC 
ACCCTCTTCT TAGAGGCTAA ACTAGAGGAG AACAAGGCAG AATCAGACAT CATTCTGCCC 
AGCACAGGCT GTGCTCCAGC CCAGGAACAC CCTCCTTCCA GTGATAGCAG CGTCTCAGAA 
CAGATCCCGA CAGCAGTGGT AGATGAAGTG CCAGAACCAG CTCCTGTGCC ATTGCTTGGA 
AGTGTTAAGA GTGCTTTCAC CCGCGTAGCC TCAACAGCTC CCTTTCGGCC TACAGAGACC 
CCAGCCCTGG AGAAGAGCCT GGCAGAAATG AGTGCTGTGT TACAGGAGCT TCAACGCCTC 
TGTGCCCTGC TGCAAGAATC TAAAGAGGAA GCCGTTGGTG TCCTGCAGAG GGAAATCTGT 
GAACTGCACA CAAGACTACA GGCCCAAGAA GAAGAACATC AGGAAGCCCA AAAGGCAAAG 
GAAGCAGACA TAGAGAAGTT GAACCAGGCC TTGTGCTTGC GCCACAAGAA TGAGAAGGAA 
CTCCTGGAAG TGATACAGAA GCAGAACGAA AAGATCCTGG GGCAGATAGA CACGAGTGGC 
CAGCTCATAA ACCTCAGGGA GGAGGTGACC CAGCTTACAC GTTTACTTCG GCGGGCAGAG 
ACAGAGACTA AAGTGCTCCA GGAAGCCCTG GAAGGCCAGG TAGATCCCAG CTGTCAGCTG 
ATGGCTACTA ACTGGATCCA GGAAAAAGTG TTTCTCTCGC AGGAGGTGAA CAAACTGAGG 
GCTATGTTCC TGGAGGTGAA AAATGAGAAG AAACAGCTGA TGGACAAGTA TCTGAGCCAT 
AGGCACATCC TGGAGGAGAA TCTTCGGCGC TCTGACACAG AGTTAAAGAA ACTTGATGAC 
ACAATTCAGC ATATCTATGA GACTCTGTTG TCTATCCCAG AGGTAGTGAA GAGTTGCAAG 
GAGCTACAAG GATTGCTAGA ATTTCTGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates Astrin at the Mitotic Spindle.
The Journal of biological chemistry291(38)20055-67(2016 09)
Chu X,Chen X,Wan Q,Zheng Z,Du Q

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
eLife4(2015 Aug)
Kodani A,Yu TW,Johnson JR,Jayaraman D,Johnson TL,Al-Gazali L,Sztriha L,Partlow JN,Kim H,Krup AL,Dammermann A,Krogan NJ,Walsh CA,Reiter JF

The microtubule-associated protein astrin transgenesis rescues spermatogenesis and renal function in hypogonadic (hgn/hgn) rats.
Andrology1(2)301-7(2013 Mar)
Katayama K,Yasuda H,Tochigi Y,Suzuki H

The role of Pax6 in regulating the orientation and mode of cell division of progenitors in the mouse cerebral cortex.
Development (Cambridge, England)138(23)5067-78(2011 Dec)
Asami M,Pilz GA,Ninkovic J,Godinho L,Schroeder T,Huttner WB,Götz M

The astrin-kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment.
The Journal of cell biology192(6)959-68(2011 Mar)
Dunsch AK,Linnane E,Barr FA,Gruneberg U

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Spag5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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