Abcf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcf1
Entrez Gene ID	224742
Full Name	ATP-binding cassette, sub-family F (GCN20), member 1
Synonyms	AU041969,Abc50,D17Wsu166e,GCN20
General protein information	Preferred Names ATP-binding cassette, sub-family F (GCN20), member 1 Names ATP-binding cassette sub-family F member 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013854.1	NP_038882.1	ATP-binding cassette sub-family F member 1
XM_006524122.2	XP_006524185.2	ATP-binding cassette sub-family F member 1 isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0441500	NP_001050461.1
Mus musculus (house mouse)	Abcf1	NP_038882.1
Arabidopsis thaliana (thale cress)	GCN4	NP_567001.1
Rattus norvegicus (Norway rat)	Abcf1	NP_001103353.1
Danio rerio (zebrafish)	abcf1	NP_998351.1
Drosophila melanogaster (fruit fly)	CG1703	NP_572736.1
Macaca mulatta (Rhesus monkey)	ABCF1	NP_001098632.1
Canis lupus familiaris (dog)	ABCF1	XP_532056.4
Caenorhabditis elegans (roundworm)	abcf-1	NP_506192.1
Homo sapiens (human)	ABCF1	NP_001081.1
Pan troglodytes (chimpanzee)	ABCF1	NP_001035838.1
Bos taurus (cattle)	ABCF1	NP_001193838.1
Xenopus tropicalis (tropical clawed frog)	abcf1	XP_002939514.2

Gene Ontology
Bibliography

Related articles in PubMed

The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.
Arora H, Wilcox SM, Johnson LA, Munro L, Eyford BA, Pfeifer CG, Welch I, Jefferies WA
Immunity50(2)418-431.e6(2019 02)

The role of the innate immune response regulatory gene ABCF1 in mammalian embryogenesis and development.
Wilcox SM, Arora H, Munro L, Xin J, Fenninger F, Johnson LA, Pfeifer CG, Choi KB, Hou J, Hoodless PA, Jefferies WA
PloS one12(5)e0175918(2017)

Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs.
Pan J, Mor G, Ju W, Zhong J, Luo X, Aldo PB, Zhong M, Yu Y, Jenkins EC, Brown WT, Zhong N
American journal of reproductive immunology (New York, N.Y. : 1989)74(3)237-57(2015 Sep)

Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro.
Bae S, Ha TS, Yoon Y, Lee J, Cha HJ, Yoo H, Choe TB, Li S, Sohn I, Kim JY, Kim CS, Jin HO, Lee HC, Park IC, Kim CS, Jin YW, Ahn SK
International journal of molecular medicine21(3)381-6(2008 Mar)

Phosphoproteomic analysis of the developing mouse brain.
Ballif BA, Villén J, Beausoleil SA, Schwartz D, Gygi SP
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Abcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu73202	XM_006524122.2 Latest version!	Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu10064	NM_013854.1 Latest version!	Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcf1 ( XM_006524122.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524185.2 Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73202
Clone ID Related Accession (Same CDS sequence)	XM_006524122.2
Accession Version	XM_006524122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family F member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAGG GTCCCAAGCA ACAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAGGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGAAAAAAGG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAAG AGCTGGCAGT AGAGGACAAA CAAGCTGGGG AAGAGGAAAA ATTGCAGAAG 
GAGAAGGAGC AGCAACAGCA ACAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGC 
CGTCGGAAAA AGGATGTGGA CGATGATAGT GATGAGAGAG TGCTCATGGA GCGACTTAAG 
CAACTGTCTG TGCCAGCCAG TGATGAGGAA GATGAGGTGC CTGCCCCCAT ACCCCGAGGA 
CGGAAGAAGG CCAAGGGTGG AAATGTTTTT GAAGCCCTGA TTCAGGATGA CAGTGAGGAG 
GAGGAAGAGG AGGAAGAAAA CCGTGTTCTC AAGCCCGCCA AGCCAGAGAA GAATCGCATC 
AATAAAGCCG TGGCTGAGGA ACCTCCTGGG CTCAGGAGTA AAAAGGGAAA GGAGGAGAAA 
TCAAAAGGGA AAGCCAAGGT GAGAGGGGGC CCTGCAGGTG GTGCTGACTG CTCCTCAGGG 
AGCCAGCCCT CCATCAGGCC TGTCTTCTCC TTGCTGCCTC AGAGTAAACC TGCTGCTGCA 
GACAGTGAAG GGGAAGAGGA GGAGGAGGAC ACAGCTAAAG AAAAGGAGCC TCCTCAGCAA 
GGGAAGGACA GAGACAAAAA GGAGGCTGAG CAGGGCTCAG GGGAAGAGAA GGAAGAGAAA 
GAAGGGGACT TGAAGGCAAA CGATCCCTAT GCCAACCTTA GCAAAAAGGA AAAGAAAAAG 
CTAAAGAAAC AGATGGATTA TGAACGACAG GTGGAGTCAT TGAAAGCAGC TAATGCTGCA 
GAAAACGACT TCTCTGTGTC CCAGGCAGAG GTGTCTTCCC GCCAGGCAAT GTTAGAAAAT 
GCATCTGACA TTAAGTTGGA AAAGTTCAGC ATCTCCGCCC ACGGCAAGGA GCTATTCGTC 
AATGCTGACC TGTACATAGT AGCCGGCCGC CGCTATGGGC TGGTGGGACC CAACGGCAAA 
GGCAAAACCA CGCTTCTGAA GCACATTGCC AACCGTGCCC TGAGCATCCC CCCTAACATT 
GACGTGCTGC TGTGCGAGCA GGAGGTGGTG GCTGATGAAA CACCAGCCGT GCAAGCTGTC 
CTTCGAGCAG ATACCAAGCG ACTGAGGTTG CTAGAGGAGG AGAGACGGCT TCAGGGACAG 
CTGGAGCAGG GGGATGACAC TGCTGCTGAG AAACTAGAAA AGGTGTATGA GGAACTGCGA 
GCTACCGGGG CAGCAGCTGC AGAGGCCAAG GCACGGCGGA TCCTGGCTGG CTTGGGCTTC 
GACCCTGAGA TGCAGAATCG GCCCACACAG AAGTTCTCTG GGGGTTGGAG AATGCGTGTC 
TCCCTGGCCA GGGCACTGTT CATGGAGCCA ACGCTGCTGA TGTTGGATGA GCCCACTAAC 
CACCTGGACC TCAACGCCGT CATCTGGCTC AATAACTACC TTCAGGGCTG GAGGAAGACG 
TTGCTGATTG TCTCCCACGA CCAGGGCTTT CTGGATGACG TTTGCACTGA TATCATCCAC 
CTGGACACCC AGCGGCTCCA TTACTACAGG GGCAATTACA TGACCTTCAA GAAGATGTAC 
CAGCAGAAGC AGAAAGAGCT GCTAAAGCAG TACGAGAAGC AGGAGAAGAA ACTGAAGGAG 
CTGAAGGCTG GGGGCAAGTC CACCAAGCAA GCGGAAAAGC AAACAAAGGA AGTCCTGACT 
CGAAAACAGC AGAAGTGCCG ACGGAAAAAC CAGGATGAAG AGTCTCAGGA GCCCCCTGAG 
CTCCTGAAGC GTCCCAAGGA GTACACCGTG CGCTTCACCT TCCCAGACCC CCCGCCTCTC 
AGCCCACCTG TGCTGGGCCT GCACGGTGTG ACGTTTGGCT ACGAGGGGCA GAAGCCACTC 
TTTAAGAATC TAGATTTCGG CATCGACATG GACTCCCGGA TTTGCATCGT GGGTCCCAAT 
GGTGTGGGGA AGAGCACACT ACTCCTGCTG CTGACTGGCA AGCTGACACC GACCAACGGG 
GAGATGAGGA AGAACCATCG GCTGAAAATC GGCTTCTTTA ACCAGCAGTA CGCAGAGCAG 
CTGCACATGG AGGAGACGCC CACTGAGTAC CTGCAGCGGA GCTTCAATCT GCCCTACCAG 
GATGCCCGGA AGTGCTTGGG CCGCTTTGGC CTGGAGAGCC ACGCCCACAC CATCCAGATC 
TGCAAACTCT CCGGTGGGCA GAAAGCCCGA GTTGTGTTTG CGGAGCTGGC CTGTCGGGAG 
CCTGATGTCC TCATCTTGGA TGAACCAACC AATAACTTGG ACATAGAGTC CATCGATGCC 
CTGGGGGAGG CCATCAACGA CTACAAGGGG GCTGTGATCG TTGTCAGCCA CGATGCGCGC 
CTCATCACAG AAACCAACTG CCAGTTGTGG GTGGTGGAGG AGCAGGGTGT CAGTCAGATC 
GACGGCGACT TTGATGACTA CAAGCGAGAG GTGTTGGAGG CCCTGGGTGA GGTCATGGTC 
AACCGTCCTC GGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524185.2
CDS	83..2680
Translation	MPKGPKQQPPEPEWIGDGEGTSPADKVVKKGKKDKKTKKTFFEELAVEDKQAGEEEKLQKEKEQQQQQQQQKKKRDTRKGRRKKDVDDDSDERVLMERLKQLSVPASDEEDEVPAPIPRGRKKAKGGNVFEALIQDDSEEEEEEEENRVLKPAKPEKNRINKAVAEEPPGLRSKKGKEEKSKGKAKVRGGPAGGADCSSGSQPSIRPVFSLLPQSKPAAADSEGEEEEEDTAKEKEPPQQGKDRDKKEAEQGSGEEKEEKEGDLKANDPYANLSKKEKKKLKKQMDYERQVESLKAANAAENDFSVSQAEVSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLRLLEEERRLQGQLEQGDDTAAEKLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDTQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEVLTRKQQKCRRKNQDEESQEPPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYEGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTNGEMRKNHRLKIGFFNQQYAEQLHMEETPTEYLQRSFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINDYKGAVIVVSHDARLITETNCQLWVVEEQGVSQIDGDFDDYKREVLEALGEVMVNRPRD

Target ORF information:

RefSeq Version	XM_006524122.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524122.2

ORF Insert Sequence:

ATGCCGAAGG GTCCCAAGCA ACAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAGGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGAAAAAAGG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAAG AGCTGGCAGT AGAGGACAAA CAAGCTGGGG AAGAGGAAAA ATTGCAGAAG 
GAGAAGGAGC AGCAACAGCA ACAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGC 
CGTCGGAAAA AGGATGTGGA CGATGATAGT GATGAGAGAG TGCTCATGGA GCGACTTAAG 
CAACTGTCTG TGCCAGCCAG TGATGAGGAA GATGAGGTGC CTGCCCCCAT ACCCCGAGGA 
CGGAAGAAGG CCAAGGGTGG AAATGTTTTT GAAGCCCTGA TTCAGGATGA CAGTGAGGAG 
GAGGAAGAGG AGGAAGAAAA CCGTGTTCTC AAGCCCGCCA AGCCAGAGAA GAATCGCATC 
AATAAAGCCG TGGCTGAGGA ACCTCCTGGG CTCAGGAGTA AAAAGGGAAA GGAGGAGAAA 
TCAAAAGGGA AAGCCAAGGT GAGAGGGGGC CCTGCAGGTG GTGCTGACTG CTCCTCAGGG 
AGCCAGCCCT CCATCAGGCC TGTCTTCTCC TTGCTGCCTC AGAGTAAACC TGCTGCTGCA 
GACAGTGAAG GGGAAGAGGA GGAGGAGGAC ACAGCTAAAG AAAAGGAGCC TCCTCAGCAA 
GGGAAGGACA GAGACAAAAA GGAGGCTGAG CAGGGCTCAG GGGAAGAGAA GGAAGAGAAA 
GAAGGGGACT TGAAGGCAAA CGATCCCTAT GCCAACCTTA GCAAAAAGGA AAAGAAAAAG 
CTAAAGAAAC AGATGGATTA TGAACGACAG GTGGAGTCAT TGAAAGCAGC TAATGCTGCA 
GAAAACGACT TCTCTGTGTC CCAGGCAGAG GTGTCTTCCC GCCAGGCAAT GTTAGAAAAT 
GCATCTGACA TTAAGTTGGA AAAGTTCAGC ATCTCCGCCC ACGGCAAGGA GCTATTCGTC 
AATGCTGACC TGTACATAGT AGCCGGCCGC CGCTATGGGC TGGTGGGACC CAACGGCAAA 
GGCAAAACCA CGCTTCTGAA GCACATTGCC AACCGTGCCC TGAGCATCCC CCCTAACATT 
GACGTGCTGC TGTGCGAGCA GGAGGTGGTG GCTGATGAAA CACCAGCCGT GCAAGCTGTC 
CTTCGAGCAG ATACCAAGCG ACTGAGGTTG CTAGAGGAGG AGAGACGGCT TCAGGGACAG 
CTGGAGCAGG GGGATGACAC TGCTGCTGAG AAACTAGAAA AGGTGTATGA GGAACTGCGA 
GCTACCGGGG CAGCAGCTGC AGAGGCCAAG GCACGGCGGA TCCTGGCTGG CTTGGGCTTC 
GACCCTGAGA TGCAGAATCG GCCCACACAG AAGTTCTCTG GGGGTTGGAG AATGCGTGTC 
TCCCTGGCCA GGGCACTGTT CATGGAGCCA ACGCTGCTGA TGTTGGATGA GCCCACTAAC 
CACCTGGACC TCAACGCCGT CATCTGGCTC AATAACTACC TTCAGGGCTG GAGGAAGACG 
TTGCTGATTG TCTCCCACGA CCAGGGCTTT CTGGATGACG TTTGCACTGA TATCATCCAC 
CTGGACACCC AGCGGCTCCA TTACTACAGG GGCAATTACA TGACCTTCAA GAAGATGTAC 
CAGCAGAAGC AGAAAGAGCT GCTAAAGCAG TACGAGAAGC AGGAGAAGAA ACTGAAGGAG 
CTGAAGGCTG GGGGCAAGTC CACCAAGCAA GCGGAAAAGC AAACAAAGGA AGTCCTGACT 
CGAAAACAGC AGAAGTGCCG ACGGAAAAAC CAGGATGAAG AGTCTCAGGA GCCCCCTGAG 
CTCCTGAAGC GTCCCAAGGA GTACACCGTG CGCTTCACCT TCCCAGACCC CCCGCCTCTC 
AGCCCACCTG TGCTGGGCCT GCACGGTGTG ACGTTTGGCT ACGAGGGGCA GAAGCCACTC 
TTTAAGAATC TAGATTTCGG CATCGACATG GACTCCCGGA TTTGCATCGT GGGTCCCAAT 
GGTGTGGGGA AGAGCACACT ACTCCTGCTG CTGACTGGCA AGCTGACACC GACCAACGGG 
GAGATGAGGA AGAACCATCG GCTGAAAATC GGCTTCTTTA ACCAGCAGTA CGCAGAGCAG 
CTGCACATGG AGGAGACGCC CACTGAGTAC CTGCAGCGGA GCTTCAATCT GCCCTACCAG 
GATGCCCGGA AGTGCTTGGG CCGCTTTGGC CTGGAGAGCC ACGCCCACAC CATCCAGATC 
TGCAAACTCT CCGGTGGGCA GAAAGCCCGA GTTGTGTTTG CGGAGCTGGC CTGTCGGGAG 
CCTGATGTCC TCATCTTGGA TGAACCAACC AATAACTTGG ACATAGAGTC CATCGATGCC 
CTGGGGGAGG CCATCAACGA CTACAAGGGG GCTGTGATCG TTGTCAGCCA CGATGCGCGC 
CTCATCACAG AAACCAACTG CCAGTTGTGG GTGGTGGAGG AGCAGGGTGT CAGTCAGATC 
GACGGCGACT TTGATGACTA CAAGCGAGAG GTGTTGGAGG CCCTGGGTGA GGTCATGGTC 
AACCGTCCTC GGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcf1 ( NM_013854.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038882.1 Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), mRNA.

Price & Availability↑

CloneID	OMu10064
Clone ID Related Accession (Same CDS sequence)	NM_013854.1
Accession Version	NM_013854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC063094.1. On Jan 1, 2004 this sequence version replaced XM_128626.4. ##Evidence-Data-START## Transcript exon combination :: BC063094.1, SRR7345562.4341715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGCCGAAGG GTCCCAAGCA ACAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAGGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGAAAAAAGG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAAG AGCTGGCAGT AGAGGACAAA CAAGCTGGGG AAGAGGAAAA ATTGCAGAAG 
GAGAAGGAGC AGCAACAGCA ACAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGC 
CGTCGGAAAA AGGATGTGGA CGATGATAGT GATGAGAGAG TGCTCATGGA GCGACTTAAG 
CAACTGTCTG TGCCAGCCAG TGATGAGGAA GATGAGGTGC CTGCCCCCAT ACCCCGAGGA 
CGGAAGAAGG CCAAGGGTGG AAATGTTTTT GAAGCCCTGA TTCAGGATGA CAGTGAGGAG 
GAGGAAGAGG AGGAAGAAAA CCGTGTTCTC AAGCCCGCCA AGCCAGAGAA GAATCGCATC 
AATAAAGCCG TGGCTGAGGA ACCTCCTGGG CTCAGGAGTA AAAAGGGAAA GGAGGAGAAA 
TCAAAAGGGA AAGCCAAGAG TAAACCTGCT GCTGCAGACA GTGAAGGGGA AGAGGAGGAG 
GAGGACACAG CTAAAGAAAA GGAGCCTCCT CAGCAAGGGA AGGACAGAGA CAAAAAGGAG 
GCTGAGCAGG GCTCAGGGGA AGAGAAGGAA GAGAAAGAAG GGGACTTGAA GGCAAACGAT 
CCCTATGCCA ACCTTAGCAA AAAGGAAAAG AAAAAGCTAA AGAAACAGAT GGATTATGAA 
CGACAGGTGG AGTCATTGAA AGCAGCTAAT GCTGCAGAAA ACGACTTCTC TGTGTCCCAG 
GCAGAGGTGT CTTCCCGCCA GGCAATGTTA GAAAATGCAT CTGACATTAA GTTGGAAAAG 
TTCAGCATCT CCGCCCACGG CAAGGAGCTA TTCGTCAATG CTGACCTGTA CATAGTAGCC 
GGCCGCCGCT ATGGGCTGGT GGGACCCAAC GGCAAAGGCA AAACCACGCT TCTGAAGCAC 
ATTGCCAACC GTGCCCTGAG CATCCCCCCT AACATTGACG TGCTGCTGTG CGAGCAGGAG 
GTGGTGGCTG ATGAAACACC AGCCGTGCAA GCTGTCCTTC GAGCAGATAC CAAGCGACTG 
AGGTTGCTAG AGGAGGAGAG ACGGCTTCAG GGACAGCTGG AGCAGGGGGA TGACACTGCT 
GCTGAGAAAC TAGAAAAGGT GTATGAGGAA CTGCGAGCTA CCGGGGCAGC AGCTGCAGAG 
GCCAAGGCAC GGCGGATCCT GGCTGGCTTG GGCTTCGACC CTGAGATGCA GAATCGGCCC 
ACACAGAAGT TCTCTGGGGG TTGGAGAATG CGTGTCTCCC TGGCCAGGGC ACTGTTCATG 
GAGCCAACGC TGCTGATGTT GGATGAGCCC ACTAACCACC TGGACCTCAA CGCCGTCATC 
TGGCTCAATA ACTACCTTCA GGGCTGGAGG AAGACGTTGC TGATTGTCTC CCACGACCAG 
GGCTTTCTGG ATGACGTTTG CACTGATATC ATCCACCTGG ACACCCAGCG GCTCCATTAC 
TACAGGGGCA ATTACATGAC CTTCAAGAAG ATGTACCAGC AGAAGCAGAA AGAGCTGCTA 
AAGCAGTACG AGAAGCAGGA GAAGAAACTG AAGGAGCTGA AGGCTGGGGG CAAGTCCACC 
AAGCAAGCGG AAAAGCAAAC AAAGGAAGTC CTGACTCGAA AACAGCAGAA GTGCCGACGG 
AAAAACCAGG ATGAAGAGTC TCAGGAGCCC CCTGAGCTCC TGAAGCGTCC CAAGGAGTAC 
ACCGTGCGCT TCACCTTCCC AGACCCCCCG CCTCTCAGCC CACCTGTGCT GGGCCTGCAC 
GGTGTGACGT TTGGCTACGA GGGGCAGAAG CCACTCTTTA AGAATCTAGA TTTCGGCATC 
GACATGGACT CCCGGATTTG CATCGTGGGT CCCAATGGTG TGGGGAAGAG CACACTACTC 
CTGCTGCTGA CTGGCAAGCT GACACCGACC AACGGGGAGA TGAGGAAGAA CCATCGGCTG 
AAAATCGGCT TCTTTAACCA GCAGTACGCA GAGCAGCTGC ACATGGAGGA GACGCCCACT 
GAGTACCTGC AGCGGAGCTT CAATCTGCCC TACCAGGATG CCCGGAAGTG CTTGGGCCGC 
TTTGGCCTGG AGAGCCACGC CCACACCATC CAGATCTGCA AACTCTCCGG TGGGCAGAAA 
GCCCGAGTTG TGTTTGCGGA GCTGGCCTGT CGGGAGCCTG ATGTCCTCAT CTTGGATGAA 
CCAACCAATA ACTTGGACAT AGAGTCCATC GATGCCCTGG GGGAGGCCAT CAACGACTAC 
AAGGGGGCTG TGATCGTTGT CAGCCACGAT GCGCGCCTCA TCACAGAAAC CAACTGCCAG 
TTGTGGGTGG TGGAGGAGCA GGGTGTCAGT CAGATCGACG GCGACTTTGA TGACTACAAG 
CGAGAGGTGT TGGAGGCCCT GGGTGAGGTC ATGGTCAACC GTCCTCGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038882.1
CDS	33..2546
Translation	MPKGPKQQPPEPEWIGDGEGTSPADKVVKKGKKDKKTKKTFFEELAVEDKQAGEEEKLQKEKEQQQQQQQQKKKRDTRKGRRKKDVDDDSDERVLMERLKQLSVPASDEEDEVPAPIPRGRKKAKGGNVFEALIQDDSEEEEEEEENRVLKPAKPEKNRINKAVAEEPPGLRSKKGKEEKSKGKAKSKPAAADSEGEEEEEDTAKEKEPPQQGKDRDKKEAEQGSGEEKEEKEGDLKANDPYANLSKKEKKKLKKQMDYERQVESLKAANAAENDFSVSQAEVSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLRLLEEERRLQGQLEQGDDTAAEKLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDTQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEVLTRKQQKCRRKNQDEESQEPPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYEGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTNGEMRKNHRLKIGFFNQQYAEQLHMEETPTEYLQRSFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINDYKGAVIVVSHDARLITETNCQLWVVEEQGVSQIDGDFDDYKREVLEALGEVMVNRPRD

Target ORF information:

RefSeq Version	NM_013854.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013854.1

ORF Insert Sequence:

ATGCCGAAGG GTCCCAAGCA ACAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAGGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGAAAAAAGG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAAG AGCTGGCAGT AGAGGACAAA CAAGCTGGGG AAGAGGAAAA ATTGCAGAAG 
GAGAAGGAGC AGCAACAGCA ACAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGC 
CGTCGGAAAA AGGATGTGGA CGATGATAGT GATGAGAGAG TGCTCATGGA GCGACTTAAG 
CAACTGTCTG TGCCAGCCAG TGATGAGGAA GATGAGGTGC CTGCCCCCAT ACCCCGAGGA 
CGGAAGAAGG CCAAGGGTGG AAATGTTTTT GAAGCCCTGA TTCAGGATGA CAGTGAGGAG 
GAGGAAGAGG AGGAAGAAAA CCGTGTTCTC AAGCCCGCCA AGCCAGAGAA GAATCGCATC 
AATAAAGCCG TGGCTGAGGA ACCTCCTGGG CTCAGGAGTA AAAAGGGAAA GGAGGAGAAA 
TCAAAAGGGA AAGCCAAGAG TAAACCTGCT GCTGCAGACA GTGAAGGGGA AGAGGAGGAG 
GAGGACACAG CTAAAGAAAA GGAGCCTCCT CAGCAAGGGA AGGACAGAGA CAAAAAGGAG 
GCTGAGCAGG GCTCAGGGGA AGAGAAGGAA GAGAAAGAAG GGGACTTGAA GGCAAACGAT 
CCCTATGCCA ACCTTAGCAA AAAGGAAAAG AAAAAGCTAA AGAAACAGAT GGATTATGAA 
CGACAGGTGG AGTCATTGAA AGCAGCTAAT GCTGCAGAAA ACGACTTCTC TGTGTCCCAG 
GCAGAGGTGT CTTCCCGCCA GGCAATGTTA GAAAATGCAT CTGACATTAA GTTGGAAAAG 
TTCAGCATCT CCGCCCACGG CAAGGAGCTA TTCGTCAATG CTGACCTGTA CATAGTAGCC 
GGCCGCCGCT ATGGGCTGGT GGGACCCAAC GGCAAAGGCA AAACCACGCT TCTGAAGCAC 
ATTGCCAACC GTGCCCTGAG CATCCCCCCT AACATTGACG TGCTGCTGTG CGAGCAGGAG 
GTGGTGGCTG ATGAAACACC AGCCGTGCAA GCTGTCCTTC GAGCAGATAC CAAGCGACTG 
AGGTTGCTAG AGGAGGAGAG ACGGCTTCAG GGACAGCTGG AGCAGGGGGA TGACACTGCT 
GCTGAGAAAC TAGAAAAGGT GTATGAGGAA CTGCGAGCTA CCGGGGCAGC AGCTGCAGAG 
GCCAAGGCAC GGCGGATCCT GGCTGGCTTG GGCTTCGACC CTGAGATGCA GAATCGGCCC 
ACACAGAAGT TCTCTGGGGG TTGGAGAATG CGTGTCTCCC TGGCCAGGGC ACTGTTCATG 
GAGCCAACGC TGCTGATGTT GGATGAGCCC ACTAACCACC TGGACCTCAA CGCCGTCATC 
TGGCTCAATA ACTACCTTCA GGGCTGGAGG AAGACGTTGC TGATTGTCTC CCACGACCAG 
GGCTTTCTGG ATGACGTTTG CACTGATATC ATCCACCTGG ACACCCAGCG GCTCCATTAC 
TACAGGGGCA ATTACATGAC CTTCAAGAAG ATGTACCAGC AGAAGCAGAA AGAGCTGCTA 
AAGCAGTACG AGAAGCAGGA GAAGAAACTG AAGGAGCTGA AGGCTGGGGG CAAGTCCACC 
AAGCAAGCGG AAAAGCAAAC AAAGGAAGTC CTGACTCGAA AACAGCAGAA GTGCCGACGG 
AAAAACCAGG ATGAAGAGTC TCAGGAGCCC CCTGAGCTCC TGAAGCGTCC CAAGGAGTAC 
ACCGTGCGCT TCACCTTCCC AGACCCCCCG CCTCTCAGCC CACCTGTGCT GGGCCTGCAC 
GGTGTGACGT TTGGCTACGA GGGGCAGAAG CCACTCTTTA AGAATCTAGA TTTCGGCATC 
GACATGGACT CCCGGATTTG CATCGTGGGT CCCAATGGTG TGGGGAAGAG CACACTACTC 
CTGCTGCTGA CTGGCAAGCT GACACCGACC AACGGGGAGA TGAGGAAGAA CCATCGGCTG 
AAAATCGGCT TCTTTAACCA GCAGTACGCA GAGCAGCTGC ACATGGAGGA GACGCCCACT 
GAGTACCTGC AGCGGAGCTT CAATCTGCCC TACCAGGATG CCCGGAAGTG CTTGGGCCGC 
TTTGGCCTGG AGAGCCACGC CCACACCATC CAGATCTGCA AACTCTCCGG TGGGCAGAAA 
GCCCGAGTTG TGTTTGCGGA GCTGGCCTGT CGGGAGCCTG ATGTCCTCAT CTTGGATGAA 
CCAACCAATA ACTTGGACAT AGAGTCCATC GATGCCCTGG GGGAGGCCAT CAACGACTAC 
AAGGGGGCTG TGATCGTTGT CAGCCACGAT GCGCGCCTCA TCACAGAAAC CAACTGCCAG 
TTGTGGGTGG TGGAGGAGCA GGGTGTCAGT CAGATCGACG GCGACTTTGA TGACTACAAG 
CGAGAGGTGT TGGAGGCCCT GGGTGAGGTC ATGGTCAACC GTCCTCGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.
Immunity50(2)418-431.e6(2019 02)
Arora H,Wilcox SM,Johnson LA,Munro L,Eyford BA,Pfeifer CG,Welch I,Jefferies WA

The role of the innate immune response regulatory gene ABCF1 in mammalian embryogenesis and development.
PloS one12(5)e0175918(2017)
Wilcox SM,Arora H,Munro L,Xin J,Fenninger F,Johnson LA,Pfeifer CG,Choi KB,Hou J,Hoodless PA,Jefferies WA

Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs.
American journal of reproductive immunology (New York, N.Y. : 1989)74(3)237-57(2015 Sep)
Pan J,Mor G,Ju W,Zhong J,Luo X,Aldo PB,Zhong M,Yu Y,Jenkins EC,Brown WT,Zhong N

Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro.
International journal of molecular medicine21(3)381-6(2008 Mar)
Bae S,Ha TS,Yoon Y,Lee J,Cha HJ,Yoo H,Choe TB,Li S,Sohn I,Kim JY,Kim CS,Jin HO,Lee HC,Park IC,Kim CS,Jin YW,Ahn SK

Phosphoproteomic analysis of the developing mouse brain.
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)
Ballif BA,Villén J,Beausoleil SA,Schwartz D,Gygi SP

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Abcf1 cDNA ORF clone, Mus musculus(house mouse)

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