Vcl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vcl
Entrez Gene ID	22330
Full Name	vinculin
Synonyms	9430097D22,AA571387,AI462105,AW545629
General protein information	Preferred Names vinculin Names vinculin metavinculin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 A3\|14 11.53 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006518922.1	XP_006518985.1	vinculin isoform X1
XM_006518922.2	XP_006518985.1	vinculin isoform X1
NM_009502.4	NP_033528.3	vinculin
NM_009502.5	NP_033528.3	vinculin

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology
Bibliography

Related articles in PubMed

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.
Xia M, Luo W, Jin H, Yang Z
Development (Cambridge, England)146(13)(2019 Jul)

Tbx1 regulates extracellular matrix-cell interactions in the second heart field.
Alfano D, Altomonte A, Cortes C, Bilio M, Kelly RG, Baldini A
Human molecular genetics28(14)2295-2308(2019 07)

Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration.
Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
Biophysical journal114(7)1680-1694(2018 04)

Vinculin is required to maintain glomerular barrier integrity.
Lausecker F, Tian X, Inoue K, Wang Z, Pedigo CE, Hassan H, Liu C, Zimmer M, Jinno S, Huckle AL, Hamidi H, Ross RS, Zent R, Ballestrem C, Lennon R, Ishibe S
Kidney international93(3)643-655(2018 03)

Compound mutations in Bmpr1a and Tak1 synergize facial deformities via increased cell death.
Liu X, Hayano S, Pan H, Inagaki M, Ninomiya-Tsuji J, Sun H, Mishina Y
Genesis (New York, N.Y. : 2000)56(3)e23093(2018 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19555	NM_009502.5 Latest version!	Mus musculus vinculin (Vcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu49928	XM_006518922.2 Latest version!	Mus musculus vinculin (Vcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu49928	XM_006518922.1	Mus musculus vinculin (Vcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19555	NM_009502.4	Mus musculus vinculin (Vcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vcl ( NM_009502.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033528.3 Mus musculus vinculin (Vcl), mRNA.

Price & Availability↑

CloneID	OMu19555
Clone ID Related Accession (Same CDS sequence)	NM_009502.4 , NM_009502.5
Accession Version	NM_009502.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513353600000
Organism	Mus musculus(house mouse)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC154840.2 and AC163681.3. On Nov 17, 2006 this sequence version replaced NM_009502.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008520.1, AK077850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCAGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TATGCGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTATCCACA 
GTGAAGGCCA CTATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAG 
GCTGAAGCTG CTTCAATCAA AATCCGAACA GATGCTGGCT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033528.3
CDS	89..3289
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_009502.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus vinculin (Vcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009502.4

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCAGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TATGCGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTATCCACA 
GTGAAGGCCA CTATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAG 
GCTGAAGCTG CTTCAATCAA AATCCGAACA GATGCTGGCT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcl ( NM_009502.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033528.3 Mus musculus vinculin (Vcl), mRNA.

Price & Availability↑

CloneID	OMu19555
Clone ID Related Accession (Same CDS sequence)	NM_009502.4 , NM_009502.5
Accession Version	NM_009502.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570377600000
Organism	Mus musculus(house mouse)
Product	vinculin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154840.2 and AC163681.3. On Apr 17, 2018 this sequence version replaced NM_009502.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008520.1, AK077850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCAGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TATGCGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTATCCACA 
GTGAAGGCCA CTATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAG 
GCTGAAGCTG CTTCAATCAA AATCCGAACA GATGCTGGCT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033528.3
CDS	124..3324
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_009502.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus vinculin (Vcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009502.5

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCAGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TATGCGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTATCCACA 
GTGAAGGCCA CTATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAG 
GCTGAAGCTG CTTCAATCAA AATCCGAACA GATGCTGGCT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcl ( XM_006518922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518985.1 Mus musculus vinculin (Vcl), transcript variant X1, mRNA.

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CloneID	OMu49928
Clone ID Related Accession (Same CDS sequence)	XM_006518922.1 , XM_006518922.2
Accession Version	XM_006518922.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGCCGGG TATCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAAATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCAGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTATGCG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTATC CACAGTGAAG GCCACTATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAGGCTGAA GCTGCTTCAA TCAAAATCCG AACAGATGCT 
GGCTTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518985.1
CDS	144..3548
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAQVGIGVVAEANAADAVGFPFPSDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006518922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vinculin (Vcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518922.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGCCGGG TATCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAAATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCAGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTATGCG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTATC CACAGTGAAG GCCACTATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAGGCTGAA GCTGCTTCAA TCAAAATCCG AACAGATGCT 
GGCTTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcl ( XM_006518922.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518985.1 Mus musculus vinculin (Vcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu49928
Clone ID Related Accession (Same CDS sequence)	XM_006518922.1 , XM_006518922.2
Accession Version	XM_006518922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGCCGGG TATCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAAATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCAGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTATGCG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTATC CACAGTGAAG GCCACTATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAGGCTGAA GCTGCTTCAA TCAAAATCCG AACAGATGCT 
GGCTTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518985.1
CDS	139..3543
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAQVGIGVVAEANAADAVGFPFPSDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006518922.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus vinculin (Vcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518922.2

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG TACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TAGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAGA CCACTGAGGA TCAGATTCTG AAGAGAGATA TGCCACCAGC CTTTATTAAG 
GTTGAAAATG CTTGCACCAA GCTTGTTCAG GCAGCCCAGA TGCTTCAGTC AGACCCATAC 
TCGGTTCCTG CGCGGGATTA CCTCATTGAC GGCTCTAGGG GAATCCTTTC TGGCACATCT 
GACCTACTGC TTACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGGGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTA GTGGAAACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAGTTGACTC ACCAGGAACA CCGTGTGATG TTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCGTCCATAG ACTCCAAATT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAATGCCTCC CCAGGGGATG CTGGAGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCTTCCCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CCGCCAAAGT GGAGAATGCA GCTCGGAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCCCAGAATT GGCTGGCGGA TCCAAATGGT 
GGACCTGAGG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCAGAA 
TTATGTGATG ATCCTAAGGA GAGAGATGAC ATCCTCCGCT CCCTTGGAGA GATAGCTGCT 
CTGACCTCTA AACTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCGCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC GACGGCACTA CAGAACCTGC AGACCAAAAC CAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTCCACCTCG AGGGCAAGAT TGAACAGGCG 
CAGCGGTGGA TTGATAACCC CACAGTAGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAGGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTTGCCA AATGTGACCG TGTAGACCAG CTAACAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CCCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAGCCCAGAT GCAGGAAGCT ATGACTCAAG AGGTATCCGA TGTTTTCAGC 
GATACTACAA CTCCTATCAA GCTGTTGGCA GTAGCCGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAAAGGGCA GCCAATTTTG AAAACCATTC AGGAAGGCTT 
GGAGCCACAG CAGAGAAGGC GGCTGCTGTT GGTACTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CTGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTCATCTC CGCTGCTCGG 
ATCTTACTGA GGAACCCTGG TAACCAGGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAGGCTAT TGATACCAAG 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGACCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTGATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGAC TAACTGATGA GCTGGCTCCT 
CCTAAGCCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCCCCACC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCT GGTGAGGTGA TTAACCAGCC AATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAATGGTCTA GCAAGCCGGG TATCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAAATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCAGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTATGCG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTATC CACAGTGAAG GCCACTATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAGGCTGAA GCTGCTTCAA TCAAAATCCG AACAGATGCT 
GGCTTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis.
Development (Cambridge, England)146(13)(2019 Jul)
Xia M,Luo W,Jin H,Yang Z

Tbx1 regulates extracellular matrix-cell interactions in the second heart field.
Human molecular genetics28(14)2295-2308(2019 07)
Alfano D,Altomonte A,Cortes C,Bilio M,Kelly RG,Baldini A

Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration.
Biophysical journal114(7)1680-1694(2018 04)
Rothenberg KE,Scott DW,Christoforou N,Hoffman BD

Vinculin is required to maintain glomerular barrier integrity.
Kidney international93(3)643-655(2018 03)
Lausecker F,Tian X,Inoue K,Wang Z,Pedigo CE,Hassan H,Liu C,Zimmer M,Jinno S,Huckle AL,Hamidi H,Ross RS,Zent R,Ballestrem C,Lennon R,Ishibe S

Compound mutations in Bmpr1a and Tak1 synergize facial deformities via increased cell death.
Genesis (New York, N.Y. : 2000)56(3)e23093(2018 03)
Liu X,Hayano S,Pan H,Inagaki M,Ninomiya-Tsuji J,Sun H,Mishina Y

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Vcl cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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