vcl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vcl
Entrez Gene ID	100036705
Full Name	vinculin
General protein information	Preferred Names vinculin Names vinculin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031904675.1	XP_031760535.1	vinculin isoform X1
XM_031904676.1	XP_031760536.1	vinculin isoform X1
XM_018095199.1	XP_017950688.1	vinculin isoform X2
XM_031904678.1	XP_031760538.1	vinculin isoform X3
NM_001097253.1	NP_001090722.1	vinculin
XM_018095198.1	XP_017950687.1	vinculin isoform X1
XM_031904677.1	XP_031760537.1	vinculin isoform X2
XM_018095200.1	XP_017950689.1	vinculin isoform X3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23459	NM_001097253.1 Latest version!	Xenopus tropicalis vinculin (vcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa23459	XM_018095199.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29895	XM_031904675.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29895	XM_031904676.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29896	XM_018095200.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29896	XM_031904677.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29897	XM_031904678.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29897	XM_018095198.1 Latest version!	Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vcl ( NM_001097253.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001090722.1 Xenopus tropicalis vinculin (vcl), mRNA.

Price & Availability↑

CloneID	OXa23459
Clone ID Related Accession (Same CDS sequence)	XM_018095199.1 , NM_001097253.1
Accession Version	NM_001097253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC127288.1. ##Evidence-Data-START## Transcript exon combination :: BC127288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090722.1
CDS	617..3814
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	NM_001097253.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097253.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_018095199.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950688.1 Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa23459
Clone ID Related Accession (Same CDS sequence)	XM_018095199.1 , NM_001097253.1
Accession Version	XM_018095199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950688.1
CDS	168..3365
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_018095199.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095199.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_031904675.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760535.1 Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29895
Clone ID Related Accession (Same CDS sequence)	XM_031904675.1 , XM_031904676.1
Accession Version	XM_031904675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGTCACC TGGAAACTCT 
GACTACCCTG CTCCACAGGG TAGAGAAGCC GTTGTATCTG AGGTAGAGCA GGAGCAAGAG 
GAGGAGGAGG AGGAGGCGTC AGTGGAATTT GCCCTCTCCT CTGATATTGA AGATGATTAT 
GAGCCAGAGC TGCTCTTAGT GCCTGAGGGG CAACCTGTAA ACCAGCCAAT GCTGGCGGCT 
GCTCAGTCTT TGCATCGGGA AGCCACAAAA TGGTCCAGTA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760535.1
CDS	611..4048
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKSPGNSDYPAPQGREAVVSEVEQEQEEEEEEASVEFALSSDIEDDYEPELLLVPEGQPVNQPMLAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_031904675.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904675.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGTCACC TGGAAACTCT 
GACTACCCTG CTCCACAGGG TAGAGAAGCC GTTGTATCTG AGGTAGAGCA GGAGCAAGAG 
GAGGAGGAGG AGGAGGCGTC AGTGGAATTT GCCCTCTCCT CTGATATTGA AGATGATTAT 
GAGCCAGAGC TGCTCTTAGT GCCTGAGGGG CAACCTGTAA ACCAGCCAAT GCTGGCGGCT 
GCTCAGTCTT TGCATCGGGA AGCCACAAAA TGGTCCAGTA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_031904676.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760536.1 Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29895
Clone ID Related Accession (Same CDS sequence)	XM_031904675.1 , XM_031904676.1
Accession Version	XM_031904676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGTCACC TGGAAACTCT 
GACTACCCTG CTCCACAGGG TAGAGAAGCC GTTGTATCTG AGGTAGAGCA GGAGCAAGAG 
GAGGAGGAGG AGGAGGCGTC AGTGGAATTT GCCCTCTCCT CTGATATTGA AGATGATTAT 
GAGCCAGAGC TGCTCTTAGT GCCTGAGGGG CAACCTGTAA ACCAGCCAAT GCTGGCGGCT 
GCTCAGTCTT TGCATCGGGA AGCCACAAAA TGGTCCAGTA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760536.1
CDS	161..3598
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKSPGNSDYPAPQGREAVVSEVEQEQEEEEEEASVEFALSSDIEDDYEPELLLVPEGQPVNQPMLAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_031904676.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904676.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGTCACC TGGAAACTCT 
GACTACCCTG CTCCACAGGG TAGAGAAGCC GTTGTATCTG AGGTAGAGCA GGAGCAAGAG 
GAGGAGGAGG AGGAGGCGTC AGTGGAATTT GCCCTCTCCT CTGATATTGA AGATGATTAT 
GAGCCAGAGC TGCTCTTAGT GCCTGAGGGG CAACCTGTAA ACCAGCCAAT GCTGGCGGCT 
GCTCAGTCTT TGCATCGGGA AGCCACAAAA TGGTCCAGTA AGGGTAATGA TATCATTGCA 
GCTGCAAAGC GCATGGCACT ACTTATGGCA GAGATGTCAC GACTTGTCAG AGGTGGCAGT 
GGTAATAAGA GGGCCCTGAT CCAGTGTGCT AAAGACATTG CAAAAGCCTC AGATGAGGTG 
ACAAAGCTAG CAAAAGAAGT GGCTAAGCAG TGTACAGACA AAAGGATACG AACAAACTTG 
CTTCAGGTAT GTGAGAGGAT TCCAACAATT AGCACACAAC TGAAAATCCT TAGTACAGTC 
AAAGCTACAA TGTTAGGAAG AACCAATATA AGTGATGAAG AGTCTGAGCA GGCAACAGAG 
ATGCTTGTCC ATAATGCACA AAACTTGATG CAGTCTGTGA AAGAAACTGT GAGAGAGGCT 
GAAGCTGCTT CTATTAAAAT ACGGACAGAT GCTGGGTGTA CACTACGCTG GGCCCGAAAA 
ACTCCATGGT ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_031904677.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760537.1 Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.

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CloneID	OXa29896
Clone ID Related Accession (Same CDS sequence)	XM_031904677.1 , XM_018095200.1
Accession Version	XM_031904677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGTCACCTGG AAACTCTGAC 
TACCCTGCTC CACAGGGTAG AGAAGCCGTT GTATCTGAGG TAGAGCAGGA GCAAGAGGAG 
GAGGAGGAGG AGGCGTCAGT GGAATTTGCC CTCTCCTCTG ATATTGAAGA TGATTATGAG 
CCAGAGCTGC TCTTAGTGCC TGAGGGGCAA CCTGTAAACC AGCCAATGCT GGCGGCTGCT 
CAGTCTTTGC ATCGGGAAGC CACAAAATGG TCCAGTAAGG GTAATGATAT CATTGCAGCT 
GCAAAGCGCA TGGCACTACT TATGGCAGAG ATGTCACGAC TTGTCAGAGG TGGCAGTGGT 
AATAAGAGGG CCCTGATCCA GTGTGCTAAA GACATTGCAA AAGCCTCAGA TGAGGTGACA 
AAGCTAGCAA AAGAAGTGGC TAAGCAGTGT ACAGACAAAA GGATACGAAC AAACTTGCTT 
CAGGTATGTG AGAGGATTCC AACAATTAGC ACACAACTGA AAATCCTTAG TACAGTCAAA 
GCTACAATGT TAGGAAGAAC CAATATAAGT GATGAAGAGT CTGAGCAGGC AACAGAGATG 
CTTGTCCATA ATGCACAAAA CTTGATGCAG TCTGTGAAAG AAACTGTGAG AGAGGCTGAA 
GCTGCTTCTA TTAAAATACG GACAGATGCT GGGTGTACAC TACGCTGGGC CCGAAAAACT 
CCATGGTACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760537.1
CDS	160..3594
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKSPGNSDYPAPQGREAVVSEVEQEQEEEEEEASVEFALSSDIEDDYEPELLLVPEGQPVNQPMLAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_031904677.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904677.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGTCACCTGG AAACTCTGAC 
TACCCTGCTC CACAGGGTAG AGAAGCCGTT GTATCTGAGG TAGAGCAGGA GCAAGAGGAG 
GAGGAGGAGG AGGCGTCAGT GGAATTTGCC CTCTCCTCTG ATATTGAAGA TGATTATGAG 
CCAGAGCTGC TCTTAGTGCC TGAGGGGCAA CCTGTAAACC AGCCAATGCT GGCGGCTGCT 
CAGTCTTTGC ATCGGGAAGC CACAAAATGG TCCAGTAAGG GTAATGATAT CATTGCAGCT 
GCAAAGCGCA TGGCACTACT TATGGCAGAG ATGTCACGAC TTGTCAGAGG TGGCAGTGGT 
AATAAGAGGG CCCTGATCCA GTGTGCTAAA GACATTGCAA AAGCCTCAGA TGAGGTGACA 
AAGCTAGCAA AAGAAGTGGC TAAGCAGTGT ACAGACAAAA GGATACGAAC AAACTTGCTT 
CAGGTATGTG AGAGGATTCC AACAATTAGC ACACAACTGA AAATCCTTAG TACAGTCAAA 
GCTACAATGT TAGGAAGAAC CAATATAAGT GATGAAGAGT CTGAGCAGGC AACAGAGATG 
CTTGTCCATA ATGCACAAAA CTTGATGCAG TCTGTGAAAG AAACTGTGAG AGAGGCTGAA 
GCTGCTTCTA TTAAAATACG GACAGATGCT GGGTGTACAC TACGCTGGGC CCGAAAAACT 
CCATGGTACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_018095200.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950689.1 Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.

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CloneID	OXa29896
Clone ID Related Accession (Same CDS sequence)	XM_031904677.1 , XM_018095200.1
Accession Version	XM_018095200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGTCACCTGG AAACTCTGAC 
TACCCTGCTC CACAGGGTAG AGAAGCCGTT GTATCTGAGG TAGAGCAGGA GCAAGAGGAG 
GAGGAGGAGG AGGCGTCAGT GGAATTTGCC CTCTCCTCTG ATATTGAAGA TGATTATGAG 
CCAGAGCTGC TCTTAGTGCC TGAGGGGCAA CCTGTAAACC AGCCAATGCT GGCGGCTGCT 
CAGTCTTTGC ATCGGGAAGC CACAAAATGG TCCAGTAAGG GTAATGATAT CATTGCAGCT 
GCAAAGCGCA TGGCACTACT TATGGCAGAG ATGTCACGAC TTGTCAGAGG TGGCAGTGGT 
AATAAGAGGG CCCTGATCCA GTGTGCTAAA GACATTGCAA AAGCCTCAGA TGAGGTGACA 
AAGCTAGCAA AAGAAGTGGC TAAGCAGTGT ACAGACAAAA GGATACGAAC AAACTTGCTT 
CAGGTATGTG AGAGGATTCC AACAATTAGC ACACAACTGA AAATCCTTAG TACAGTCAAA 
GCTACAATGT TAGGAAGAAC CAATATAAGT GATGAAGAGT CTGAGCAGGC AACAGAGATG 
CTTGTCCATA ATGCACAAAA CTTGATGCAG TCTGTGAAAG AAACTGTGAG AGAGGCTGAA 
GCTGCTTCTA TTAAAATACG GACAGATGCT GGGTGTACAC TACGCTGGGC CCGAAAAACT 
CCATGGTACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950689.1
CDS	611..4045
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKSPGNSDYPAPQGREAVVSEVEQEQEEEEEEASVEFALSSDIEDDYEPELLLVPEGQPVNQPMLAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_018095200.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095200.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGGACACTG AGGCCATGAA AAGAGCATTG GCTCTGATTG ATTCTAAGAT AAACCAAGCA 
AAGGGTTGGC TTCGGGATCC TAATGCTCCT CCAGGGGATG TTGGGGAACA AGCTGTGCGC 
CAGATACTAG ATGAAGCTGG AAAGGTTGGT GAGCTTTGTG CTGGAACAGA ACGGAAGGAC 
ATCCTGGGCA TCTGCAGGAC TTTAGGGCAA ATGACTGATC AAGTGTCTGA CCTAAGAGCT 
AGGGGACAAG GTGCTACTCC CATTGCCATG CAAAAAGCTC AGCAGGTGTC TCAGGGCTTG 
GATGTCCTAA CATCCAAAGT AAAGAATGCA GCTCATAAGC TGGAAGCACT GACTAATTCT 
AAGCAGGCTA TTGGCAAGAA GATTGATGCA GCTCAGAGCT GGCTGGCAGA TCCAAATGGA 
GGCCCAGAAG GAGAGGAAAA TATTCGTACT ATTCTTGCTG AGGCTAAGAA GATTGCAGAC 
TTATGTGAAG ACCCCAAAGA TAAAGAGGAT ATTTTACGTT CTCTAGGAGA GATAGCAGCT 
CTAACTGCCA AGCTCACTGA TCTCCGTCGA CAGGGAAAAG GAGACTCTCA TGAAGCTCGT 
GCTTTGGCCA AACAGATTGC AACTTCTTTA CAGAATCTGC AGACCAAGGT GAATCGTGCT 
GTGGCCAACA GCCGCCCTGT AAAGGCTGCT GTGAACATGG AAGGAAAGGT GGAACAGGCC 
CAACGCTGGA TAGATAACCC AAGTGTAGAC GACAAGGGTG TTGGTCAGGC AGCTATCCGG 
GGTTTGGTGG CAGAAGGTCG TCGGTTAGCA AACTCCATGA TTGGGCCTTT CCGCCAGGAC 
ATGATGGCAA AATGTGACCG TGTGGAGCAG CTGGCAGGGC AGCTGGCAGA GCTTGCATTA 
CGTGGTGAGG GTGACACACC ACTGGCTCAG GCTGTAGCAG CTCAGCTTCA AGAAGCACTC 
AAGGATCTAA AGGGGAAAAT GCAGGAAGCA ATGACTCAAG AGGTGTCTGA TGTTTTTAGT 
GACACAACAA CACCCATTAA ACTTTTAGCT GTTGCAGCAA CATCTCCTTC TGATACCCCC 
AACAGAGATG AGGTTTTTGA AGAAAGGGCT ACAAACTTTG AGAGCCACTC AGCACGTCTG 
GGCGCTACTG CTGAGAAGGC AGCTGCTGTA GGATCAGCTA ATAAAGCAAC TGTGGAGGGA 
ATACAAGCTG CTGTGAAATC AGCAAGGGAG CTTACTCCTC AGGTTGTTTC TGCTGCTCGC 
ATCCTACTGC GCAACCCAGG TAATCAAGCA GCCTATGAGC ACTTTGAAAC TATGAAGAAT 
CAGTGGATTG ACAATGTGGA AAAAATGACT GGCTTGGTAG ATGAGGCAAT TGACACCCGG 
TCTCTATTGG ATGCTTCTGA AGAAGCCATT AAAAAAGACA TAGATAAGTG TAAAGTTGCC 
ATGGCTAATA TGCAGCCACA GATGCTAGTC GCTGGGGCTA CTAGTATTGC CCGCCGTGCT 
AATCGCATTT TACTAGTGGC AAAAAGGGAA ATGGAAAACT CTGAAGATCC CAAGTTCCGT 
GATGCTGTAA AAAATGCTTC AGATGAGCTC AGTAAGACCA TCTCCCCTAT GGTGATGGAA 
GCTAAAGCAG TTGCTGGAAA CATCTCCAAT CCTGCCCTAC AGAAGGGTTT CTTAGACTCT 
GGATACCGAA TTTTGGGTGC TGTGGCCAAG GTTAGGGAAG CTTTTCAGCC TCCAGAGCCA 
GAATTTCCAC CACCACCTGA TCTGGACCAG CTTCGGCTTT CTGATGAGGC TGCCCCTCCA 
AAGCCACCAT TGCCAGAAGG AGAAGTTCCA CCGCCTAGGC CACCACCACC TGAGGAAAAG 
GATGAGGAAT TTCCAGAGCA GAAGGTGGGG GAGGTGGTGA ATCAGCCAAT GATGGTGGCT 
GCAAGGCAAC TGCATGATGA GGCACGAAAG TGGTCCAGCA AGTCACCTGG AAACTCTGAC 
TACCCTGCTC CACAGGGTAG AGAAGCCGTT GTATCTGAGG TAGAGCAGGA GCAAGAGGAG 
GAGGAGGAGG AGGCGTCAGT GGAATTTGCC CTCTCCTCTG ATATTGAAGA TGATTATGAG 
CCAGAGCTGC TCTTAGTGCC TGAGGGGCAA CCTGTAAACC AGCCAATGCT GGCGGCTGCT 
CAGTCTTTGC ATCGGGAAGC CACAAAATGG TCCAGTAAGG GTAATGATAT CATTGCAGCT 
GCAAAGCGCA TGGCACTACT TATGGCAGAG ATGTCACGAC TTGTCAGAGG TGGCAGTGGT 
AATAAGAGGG CCCTGATCCA GTGTGCTAAA GACATTGCAA AAGCCTCAGA TGAGGTGACA 
AAGCTAGCAA AAGAAGTGGC TAAGCAGTGT ACAGACAAAA GGATACGAAC AAACTTGCTT 
CAGGTATGTG AGAGGATTCC AACAATTAGC ACACAACTGA AAATCCTTAG TACAGTCAAA 
GCTACAATGT TAGGAAGAAC CAATATAAGT GATGAAGAGT CTGAGCAGGC AACAGAGATG 
CTTGTCCATA ATGCACAAAA CTTGATGCAG TCTGTGAAAG AAACTGTGAG AGAGGCTGAA 
GCTGCTTCTA TTAAAATACG GACAGATGCT GGGTGTACAC TACGCTGGGC CCGAAAAACT 
CCATGGTACC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_031904678.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760538.1 Xenopus tropicalis vinculin (vcl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa29897
Clone ID Related Accession (Same CDS sequence)	XM_031904678.1 , XM_018095198.1
Accession Version	XM_031904678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGGGTAA TGATATCATT 
GCAGCTGCAA AGCGCATGGC ACTACTTATG GCAGAGATGT CACGACTTGT CAGAGGTGGC 
AGTGGTAATA AGAGGGCCCT GATCCAGTGT GCTAAAGACA TTGCAAAAGC CTCAGATGAG 
GTGACAAAGC TAGCAAAAGA AGTGGCTAAG CAGTGTACAG ACAAAAGGAT ACGAACAAAC 
TTGCTTCAGG TATGTGAGAG GATTCCAACA ATTAGCACAC AACTGAAAAT CCTTAGTACA 
GTCAAAGCTA CAATGTTAGG AAGAACCAAT ATAAGTGATG AAGAGTCTGA GCAGGCAACA 
GAGATGCTTG TCCATAATGC ACAAAACTTG ATGCAGTCTG TGAAAGAAAC TGTGAGAGAG 
GCTGAAGCTG CTTCTATTAA AATACGGACA GATGCTGGGT GTACACTACG CTGGGCCCGA 
AAAACTCCAT GGTACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760538.1
CDS	611..3811
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_031904678.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904678.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGGGTAA TGATATCATT 
GCAGCTGCAA AGCGCATGGC ACTACTTATG GCAGAGATGT CACGACTTGT CAGAGGTGGC 
AGTGGTAATA AGAGGGCCCT GATCCAGTGT GCTAAAGACA TTGCAAAAGC CTCAGATGAG 
GTGACAAAGC TAGCAAAAGA AGTGGCTAAG CAGTGTACAG ACAAAAGGAT ACGAACAAAC 
TTGCTTCAGG TATGTGAGAG GATTCCAACA ATTAGCACAC AACTGAAAAT CCTTAGTACA 
GTCAAAGCTA CAATGTTAGG AAGAACCAAT ATAAGTGATG AAGAGTCTGA GCAGGCAACA 
GAGATGCTTG TCCATAATGC ACAAAACTTG ATGCAGTCTG TGAAAGAAAC TGTGAGAGAG 
GCTGAAGCTG CTTCTATTAA AATACGGACA GATGCTGGGT GTACACTACG CTGGGCCCGA 
AAAACTCCAT GGTACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcl ( XM_018095198.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950687.1 Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.

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CloneID	OXa29897
Clone ID Related Accession (Same CDS sequence)	XM_031904678.1 , XM_018095198.1
Accession Version	XM_018095198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGGGTAA TGATATCATT 
GCAGCTGCAA AGCGCATGGC ACTACTTATG GCAGAGATGT CACGACTTGT CAGAGGTGGC 
AGTGGTAATA AGAGGGCCCT GATCCAGTGT GCTAAAGACA TTGCAAAAGC CTCAGATGAG 
GTGACAAAGC TAGCAAAAGA AGTGGCTAAG CAGTGTACAG ACAAAAGGAT ACGAACAAAC 
TTGCTTCAGG TATGTGAGAG GATTCCAACA ATTAGCACAC AACTGAAAAT CCTTAGTACA 
GTCAAAGCTA CAATGTTAGG AAGAACCAAT ATAAGTGATG AAGAGTCTGA GCAGGCAACA 
GAGATGCTTG TCCATAATGC ACAAAACTTG ATGCAGTCTG TGAAAGAAAC TGTGAGAGAG 
GCTGAAGCTG CTTCTATTAA AATACGGACA GATGCTGGGT GTACACTACG CTGGGCCCGA 
AAAACTCCAT GGTACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950687.1
CDS	169..3369
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPELTAPVAAVQAAVSNLVRVGKETVQTTEDQIMKRDMPPAFIKVENACAKLVQAAQMLHADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVESMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKDLLPVLISAMKIFVTTKNSRSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALALIDSKINQAKGWLRDPNAPPGDVGEQAVRQILDEAGKVGELCAGTERKDILGICRTLGQMTDQVSDLRARGQGATPIAMQKAQQVSQGLDVLTSKVKNAAHKLEALTNSKQAIGKKIDAAQSWLADPNGGPEGEENIRTILAEAKKIADLCEDPKDKEDILRSLGEIAALTAKLTDLRRQGKGDSHEARALAKQIATSLQNLQTKVNRAVANSRPVKAAVNMEGKVEQAQRWIDNPSVDDKGVGQAAIRGLVAEGRRLANSMIGPFRQDMMAKCDRVEQLAGQLAELALRGEGDTPLAQAVAAQLQEALKDLKGKMQEAMTQEVSDVFSDTTTPIKLLAVAATSPSDTPNRDEVFEERATNFESHSARLGATAEKAAAVGSANKATVEGIQAAVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTRSLLDASEEAIKKDIDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREMENSEDPKFRDAVKNASDELSKTISPMVMEAKAVAGNISNPALQKGFLDSGYRILGAVAKVREAFQPPEPEFPPPPDLDQLRLSDEAAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKVGEVVNQPMMVAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGCTLRWARKTPWYQ

Target ORF information:

RefSeq Version	XM_018095198.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis vinculin (vcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095198.1

ORF Insert Sequence:

ATGCCGGTCT TCCACACAAA GACTATAGAG AGCATCCTGG AGCCGGTGGC CCAGCAAATC 
TCGCACTTGG TCATTATGCA TGAGGAAGGC GAAGTAGATG GCAAAGCAAT TCCGGAGCTC 
ACTGCTCCAG TAGCGGCTGT GCAGGCGGCA GTCAGCAATC TAGTGCGGGT TGGTAAAGAA 
ACTGTTCAGA CCACTGAGGA TCAGATCATG AAGCGAGATA TGCCTCCTGC ATTCATCAAG 
GTAGAAAATG CCTGTGCTAA GCTTGTTCAG GCGGCCCAGA TGCTGCATGC TGATCCATAC 
TCCGTACCAG CCCGTGATTA CCTTATTGAT GGGTCTAGAG GAATCTTGTC TGGCACTTCA 
GATCTTCTCC TAACATTTGA TGAAGCTGAG GTGAGAAAAA TTATCCGAGT CTGCAAAGGG 
ATCCTGGAAT ATCTAACAGT TGCTGAAGTG GTAGAGAGCA TGGAGGATCT TGTCACTTAC 
ACTAAGAACT TAGGCCCTGG GATGACTAAG ATGGCAAAGA TGATTGATGA ACGTCAGCAG 
GAGTTAACCC ATCAAGAACA CCGTGTGATG CTTGTAAATT CCATGAACAC TGTGAAAGAT 
CTTCTGCCAG TCCTAATTTC TGCAATGAAG ATATTCGTGA CTACTAAAAA TTCACGTAGT 
CAAGGAATTG AAGAGGCTTT AAAGAACCGC AACTTTACAG TAGAAAAAAT GAGTGCCGAA 
ATTAACGAGA TCATTAGAGT ATTGCAACTC ACTTCCTGGG ATGAAGATGC TTGGGCCAGT 
AAGAAGGACA CTGAGGCCAT GAAAAGAGCA TTGGCTCTGA TTGATTCTAA GATAAACCAA 
GCAAAGGGTT GGCTTCGGGA TCCTAATGCT CCTCCAGGGG ATGTTGGGGA ACAAGCTGTG 
CGCCAGATAC TAGATGAAGC TGGAAAGGTT GGTGAGCTTT GTGCTGGAAC AGAACGGAAG 
GACATCCTGG GCATCTGCAG GACTTTAGGG CAAATGACTG ATCAAGTGTC TGACCTAAGA 
GCTAGGGGAC AAGGTGCTAC TCCCATTGCC ATGCAAAAAG CTCAGCAGGT GTCTCAGGGC 
TTGGATGTCC TAACATCCAA AGTAAAGAAT GCAGCTCATA AGCTGGAAGC ACTGACTAAT 
TCTAAGCAGG CTATTGGCAA GAAGATTGAT GCAGCTCAGA GCTGGCTGGC AGATCCAAAT 
GGAGGCCCAG AAGGAGAGGA AAATATTCGT ACTATTCTTG CTGAGGCTAA GAAGATTGCA 
GACTTATGTG AAGACCCCAA AGATAAAGAG GATATTTTAC GTTCTCTAGG AGAGATAGCA 
GCTCTAACTG CCAAGCTCAC TGATCTCCGT CGACAGGGAA AAGGAGACTC TCATGAAGCT 
CGTGCTTTGG CCAAACAGAT TGCAACTTCT TTACAGAATC TGCAGACCAA GGTGAATCGT 
GCTGTGGCCA ACAGCCGCCC TGTAAAGGCT GCTGTGAACA TGGAAGGAAA GGTGGAACAG 
GCCCAACGCT GGATAGATAA CCCAAGTGTA GACGACAAGG GTGTTGGTCA GGCAGCTATC 
CGGGGTTTGG TGGCAGAAGG TCGTCGGTTA GCAAACTCCA TGATTGGGCC TTTCCGCCAG 
GACATGATGG CAAAATGTGA CCGTGTGGAG CAGCTGGCAG GGCAGCTGGC AGAGCTTGCA 
TTACGTGGTG AGGGTGACAC ACCACTGGCT CAGGCTGTAG CAGCTCAGCT TCAAGAAGCA 
CTCAAGGATC TAAAGGGGAA AATGCAGGAA GCAATGACTC AAGAGGTGTC TGATGTTTTT 
AGTGACACAA CAACACCCAT TAAACTTTTA GCTGTTGCAG CAACATCTCC TTCTGATACC 
CCCAACAGAG ATGAGGTTTT TGAAGAAAGG GCTACAAACT TTGAGAGCCA CTCAGCACGT 
CTGGGCGCTA CTGCTGAGAA GGCAGCTGCT GTAGGATCAG CTAATAAAGC AACTGTGGAG 
GGAATACAAG CTGCTGTGAA ATCAGCAAGG GAGCTTACTC CTCAGGTTGT TTCTGCTGCT 
CGCATCCTAC TGCGCAACCC AGGTAATCAA GCAGCCTATG AGCACTTTGA AACTATGAAG 
AATCAGTGGA TTGACAATGT GGAAAAAATG ACTGGCTTGG TAGATGAGGC AATTGACACC 
CGGTCTCTAT TGGATGCTTC TGAAGAAGCC ATTAAAAAAG ACATAGATAA GTGTAAAGTT 
GCCATGGCTA ATATGCAGCC ACAGATGCTA GTCGCTGGGG CTACTAGTAT TGCCCGCCGT 
GCTAATCGCA TTTTACTAGT GGCAAAAAGG GAAATGGAAA ACTCTGAAGA TCCCAAGTTC 
CGTGATGCTG TAAAAAATGC TTCAGATGAG CTCAGTAAGA CCATCTCCCC TATGGTGATG 
GAAGCTAAAG CAGTTGCTGG AAACATCTCC AATCCTGCCC TACAGAAGGG TTTCTTAGAC 
TCTGGATACC GAATTTTGGG TGCTGTGGCC AAGGTTAGGG AAGCTTTTCA GCCTCCAGAG 
CCAGAATTTC CACCACCACC TGATCTGGAC CAGCTTCGGC TTTCTGATGA GGCTGCCCCT 
CCAAAGCCAC CATTGCCAGA AGGAGAAGTT CCACCGCCTA GGCCACCACC ACCTGAGGAA 
AAGGATGAGG AATTTCCAGA GCAGAAGGTG GGGGAGGTGG TGAATCAGCC AATGATGGTG 
GCTGCAAGGC AACTGCATGA TGAGGCACGA AAGTGGTCCA GCAAGGGTAA TGATATCATT 
GCAGCTGCAA AGCGCATGGC ACTACTTATG GCAGAGATGT CACGACTTGT CAGAGGTGGC 
AGTGGTAATA AGAGGGCCCT GATCCAGTGT GCTAAAGACA TTGCAAAAGC CTCAGATGAG 
GTGACAAAGC TAGCAAAAGA AGTGGCTAAG CAGTGTACAG ACAAAAGGAT ACGAACAAAC 
TTGCTTCAGG TATGTGAGAG GATTCCAACA ATTAGCACAC AACTGAAAAT CCTTAGTACA 
GTCAAAGCTA CAATGTTAGG AAGAACCAAT ATAAGTGATG AAGAGTCTGA GCAGGCAACA 
GAGATGCTTG TCCATAATGC ACAAAACTTG ATGCAGTCTG TGAAAGAAAC TGTGAGAGAG 
GCTGAAGCTG CTTCTATTAA AATACGGACA GATGCTGGGT GTACACTACG CTGGGCCCGA 
AAAACTCCAT GGTACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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vcl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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