Vars cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vars
Entrez Gene ID	22321
Full Name	valyl-tRNA synthetase
Synonyms	Bat6,D17H6S56E,G7a,Vars2
General protein information	Preferred Names valyl-tRNA synthetase Names valine--tRNA ligase protein G7a valRS
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017317396.2	XP_017172885.1	valine--tRNA ligase isoform X2
NM_011690.3	NP_035820.3	valine--tRNA ligase
XM_017317395.2	XP_017172884.1	valine--tRNA ligase isoform X1
XM_017317395.1	XP_017172884.1	valine--tRNA ligase isoform X1
XM_017317396.1	XP_017172885.1	valine--tRNA ligase isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Neurospora crassa	NCU01965	XP_964329.2
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2

Gene Ontology
Bibliography

Related articles in PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Xie T, Rowen L, Aguado B, Ahearn ME, Madan A, Qin S, Campbell RD, Hood L
Genome research13(12)2621-36(2003 Dec)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Carninci P, Waki K, Shiraki T, Konno H, Shibata K, Itoh M, Aizawa K, Arakawa T, Ishii Y, Sasaki D, Bono H, Kondo S, Sugahara Y, Saito R, Osato N, Fukuda S, Sato K, Watahiki A, Hirozane-Kishikawa T, Nakamura M, Shibata Y, Yasunishi A, Kikuchi N, Yoshiki A, Kusakabe M, Gustincich S, Beisel K, Pavan W, Aidinis V, Nakagawara A, Held WA, Iwata H, Kono T, Nakauchi H, Lyons P, Wells C, Hume DA, Fagiolini M, Hensch TK, Brinkmeier M, Camper S, Hirota J, Mombaerts P, Muramatsu M, Okazaki Y, Kawai J, Hayashizaki Y
Genome research13(6B)1273-89(2003 Jun)

Genotype versus phenotype: conflicting results in mapping a lung tumor susceptibility locus to the G7c recombination interval in the mouse MHC class III region.
van Kooij M, de Groot K, van Vugt H, Aten J, Snoek M
Immunogenetics53(8)656-61(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79554	XM_017317396.2 Latest version!	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13227	NM_011690.3 Latest version!	Mus musculus valyl-tRNA synthetase (Vars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13227	XM_017317395.2 Latest version!	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu13227	XM_017317395.1	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu79554	XM_017317396.1	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vars ( XM_017317396.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172885.1 Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79554
Clone ID Related Accession (Same CDS sequence)	XM_017317396.2 , XM_017317396.1
Accession Version	XM_017317396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGG AGACCACCCT ATGGAACCCA GGCTGTGACC ATGCAGGCAT TGCCACCCAG 
GTGGTGGTGG AAAAGAAGCT CTGGAAAGAG CGGGGTCTGA ACCGGCACCA GCTGGGCCGC 
GAGGCCTTTC TTGAGGAGGT CTGGAAGTGG AAAGCCGAGA AGGGTGACAG GATTTACCAC 
CAATTAAAGA AACTTGGCAG CTCCTTGGAC TGGGATCGAG CCTGCTTCAC CATGGATCCT 
AAATTGTCAG CAACTGTGAC AGAGGCCTTT GTTAGGCTCC ACGAAGAAGG GGTCATCTAC 
CGTAGCACCC GCCTGGTCAA CTGGTCCTGC ACCCTCAACT CAGCCATTTC TGACATTGAG 
GTGGATAAGA AGGAGCTGAC AGGCCGCACC CTGCTTCCTG TGCCTGGCTA CAAGGAGAAG 
GTGGAGTTTG GGGTCCTTGT GTCCTTTGCC TACAAGGTCC AAGGCTCAGA CAGCGACGAG 
GAGGTGGTGG TGGCAACAAC CCGGATTGAG ACCATGCTGG GAGACGTGGC TGTAGCTGTG 
CACCCCAAGG ATCCCAGATA CCAGCACTTA AAGGGAAAGT GTGTCGTCCA CCCATTCTTG 
TCCCGGAGCC TTCCCATCGT CTTCGATGAC TTTGTAGACA TGGAGTTTGG CACAGGTGCC 
GTGAAGATCA CCCCGGCACA TGACCAAAAT GACTATGAGG TTGGGCAGCG ACACAGGCTC 
GAGGCCATTA GCATCATGGA CTCAAAGGGG GCCCTCATCA ATGTGCCTCC ACCTTTCCTG 
GGCCTGCCCA GGTTTGAGGC CAGGAAGGCT GTGCTGGCGG CACTGAAGGA GCGGGGCCTG 
TTCCGTGGAG TCAAGGACAA CCCCATGGTG GTGCCGCTTT GCAACCGCTC CAAGGATGTG 
GTGGAGCCTC TGTTAAGGCC ACAATGGTAC GTGCGCTGTG GGGAGATGGC TCAGGCTGCC 
AGTGCTGCTG TGACCCGGGG TGACCTCCGT ATACTGCCTG AGGCCCATCA ACGGACGTGG 
CATTCTTGGA TGGACAACAT CAGAGACTGG TGCATCTCTC GGCAGCTGTG GTGGGGCCAC 
CGAATCCCTG CCTACTTTAT CACCGTCCAT GACCCGGCAG TACCCCCTGG GGAGGACCCT 
GATGGGCGGT ACTGGGTAAG CGGACGCACT GAAGCAGAGG CCCGAGAGAA GGCAGCACGG 
GAGTTTGGAG TGTCCCCTGA CAAGATCAGC CTTCAGCAAG ATGAGGATGT GTTGGACACC 
TGGTTCTCCT CCGGTCTCTT TCCCTTCTCC ATCTTTGGCT GGCCCAATCA GTCAGAAGAC 
CTCAGTGTGT TCTACCCTGG GACCCTGTTG GAGACAGGCC ATGATATCCT CTTCTTCTGG 
GTGGCCCGGA TGGTCATGCT CGGCCTGAAG CTCACTGGGA AGCTGCCCTT CAGAGAGGTC 
TATCTCCATG CAATCGTGCG TGATGCTCAT GGCAGGAAGA TGAGCAAGTC TCTTGGCAAT 
GTCATCGACC CCCTGGATGT CATCCATGGA GTTTCCTTGC AGGGCCTCTA TGACCAACTA 
CTGAACAGCA ACCTGGATCC CAGTGAGGTG GAGAAAGCCA AAGAAGGACA GAAGGCCGAC 
TTTCCAGCAG GGATTCCCGA GTGTGGCACT GATGCCCTAC GCTTTGGACT CTGTGCCTAC 
ACATCCCAAG GTCGAGACAT CAACCTGGAT GTGAACAGGA TCCTGGGGTA CCGTCACTTC 
TGCAACAAAC TCTGGAATGC TACCAAGTTT GCCCTGCGCG GCCTTGGGAA GGGCTTTGTG 
CCCTCAGCAA CCTCCAAGCC TGAAGGGCAC GAGAGCCTGG TGGACCGCTG GATCCGCAGC 
CGCCTAACTG AGGCCGTGAG GCTCAGCAAT GAAGGCTTCC AGGCTTACGA TTTCCCGGCC 
ATCACCACCG CCCAGTACAG CTTTTGGCTC TATGAGCTCT GTGATGTCTA TTTGGAGTGT 
CTAAAACCTG TGCTGAATGG AGTGGACCAG GTGGCGGCAG AGTGTGCTCG GCAGACCCTC 
TACACCTGCC TGGATGTTGG CCTGCGGCTG CTCTCGCCCT TCATGCCCTT TGTCACAGAG 
GAGCTATTCC AGAGACTGCC CCGGCGGACA CCAAAAGCGC CTGCTAGCCT CTGTGTCACC 
CCGTACCCAG AGCCCTCAGA GTGCTCCTGG AAGGACCCTG AAGCTGAAGC TGCTCTTGAA 
CTAGCTCTGA GCATCACTCG AGCTGTGCGC TCCCTGCGTG CTGACTACAA CCTGACCCGC 
ACCAGGCCCG ACTGTTTCTT GGAAGTAGCC GATGAGGCCA CAGGTGCCTT GGCCTCGGCA 
GTGTCGGGCT ACGTGCAGGC TCTGGCCAGT GCGGGCGTGG TGGCTGTCCT CGCCCTGGGT 
GCTCCTGCAC CGCAGGGCTG CGCTGTAGCT GTGGCTTCTG ACCGCTGCTC CATCCACCTG 
CAGCTGCAGG GGCTAGTGGA CCCAGCCCGG GAGTTGGGCA AGCTGCAGGC CAAGCGAAGT 
GAGGCACAGC GGCAGGCTCA GCGGCTGCAG GAGCGCCGTG CTGCCTCCAG CTACTCAGCA 
AAGGTGCCCC TTGAGGTCCA GGAGGCAGAT GAAGCGAAGT TGCAACAGAC AGAGGCGGAG 
CTCAGGAAGG TGGATGAGGC CATCGCCCTG TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172885.1
CDS	3841..6528
Translation	MRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGLPRFEARKAVLAALKERGLFRGVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017317396.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317396.2

ORF Insert Sequence:

ATGCGTGGGG AGACCACCCT ATGGAACCCA GGCTGTGACC ATGCAGGCAT TGCCACCCAG 
GTGGTGGTGG AAAAGAAGCT CTGGAAAGAG CGGGGTCTGA ACCGGCACCA GCTGGGCCGC 
GAGGCCTTTC TTGAGGAGGT CTGGAAGTGG AAAGCCGAGA AGGGTGACAG GATTTACCAC 
CAATTAAAGA AACTTGGCAG CTCCTTGGAC TGGGATCGAG CCTGCTTCAC CATGGATCCT 
AAATTGTCAG CAACTGTGAC AGAGGCCTTT GTTAGGCTCC ACGAAGAAGG GGTCATCTAC 
CGTAGCACCC GCCTGGTCAA CTGGTCCTGC ACCCTCAACT CAGCCATTTC TGACATTGAG 
GTGGATAAGA AGGAGCTGAC AGGCCGCACC CTGCTTCCTG TGCCTGGCTA CAAGGAGAAG 
GTGGAGTTTG GGGTCCTTGT GTCCTTTGCC TACAAGGTCC AAGGCTCAGA CAGCGACGAG 
GAGGTGGTGG TGGCAACAAC CCGGATTGAG ACCATGCTGG GAGACGTGGC TGTAGCTGTG 
CACCCCAAGG ATCCCAGATA CCAGCACTTA AAGGGAAAGT GTGTCGTCCA CCCATTCTTG 
TCCCGGAGCC TTCCCATCGT CTTCGATGAC TTTGTAGACA TGGAGTTTGG CACAGGTGCC 
GTGAAGATCA CCCCGGCACA TGACCAAAAT GACTATGAGG TTGGGCAGCG ACACAGGCTC 
GAGGCCATTA GCATCATGGA CTCAAAGGGG GCCCTCATCA ATGTGCCTCC ACCTTTCCTG 
GGCCTGCCCA GGTTTGAGGC CAGGAAGGCT GTGCTGGCGG CACTGAAGGA GCGGGGCCTG 
TTCCGTGGAG TCAAGGACAA CCCCATGGTG GTGCCGCTTT GCAACCGCTC CAAGGATGTG 
GTGGAGCCTC TGTTAAGGCC ACAATGGTAC GTGCGCTGTG GGGAGATGGC TCAGGCTGCC 
AGTGCTGCTG TGACCCGGGG TGACCTCCGT ATACTGCCTG AGGCCCATCA ACGGACGTGG 
CATTCTTGGA TGGACAACAT CAGAGACTGG TGCATCTCTC GGCAGCTGTG GTGGGGCCAC 
CGAATCCCTG CCTACTTTAT CACCGTCCAT GACCCGGCAG TACCCCCTGG GGAGGACCCT 
GATGGGCGGT ACTGGGTAAG CGGACGCACT GAAGCAGAGG CCCGAGAGAA GGCAGCACGG 
GAGTTTGGAG TGTCCCCTGA CAAGATCAGC CTTCAGCAAG ATGAGGATGT GTTGGACACC 
TGGTTCTCCT CCGGTCTCTT TCCCTTCTCC ATCTTTGGCT GGCCCAATCA GTCAGAAGAC 
CTCAGTGTGT TCTACCCTGG GACCCTGTTG GAGACAGGCC ATGATATCCT CTTCTTCTGG 
GTGGCCCGGA TGGTCATGCT CGGCCTGAAG CTCACTGGGA AGCTGCCCTT CAGAGAGGTC 
TATCTCCATG CAATCGTGCG TGATGCTCAT GGCAGGAAGA TGAGCAAGTC TCTTGGCAAT 
GTCATCGACC CCCTGGATGT CATCCATGGA GTTTCCTTGC AGGGCCTCTA TGACCAACTA 
CTGAACAGCA ACCTGGATCC CAGTGAGGTG GAGAAAGCCA AAGAAGGACA GAAGGCCGAC 
TTTCCAGCAG GGATTCCCGA GTGTGGCACT GATGCCCTAC GCTTTGGACT CTGTGCCTAC 
ACATCCCAAG GTCGAGACAT CAACCTGGAT GTGAACAGGA TCCTGGGGTA CCGTCACTTC 
TGCAACAAAC TCTGGAATGC TACCAAGTTT GCCCTGCGCG GCCTTGGGAA GGGCTTTGTG 
CCCTCAGCAA CCTCCAAGCC TGAAGGGCAC GAGAGCCTGG TGGACCGCTG GATCCGCAGC 
CGCCTAACTG AGGCCGTGAG GCTCAGCAAT GAAGGCTTCC AGGCTTACGA TTTCCCGGCC 
ATCACCACCG CCCAGTACAG CTTTTGGCTC TATGAGCTCT GTGATGTCTA TTTGGAGTGT 
CTAAAACCTG TGCTGAATGG AGTGGACCAG GTGGCGGCAG AGTGTGCTCG GCAGACCCTC 
TACACCTGCC TGGATGTTGG CCTGCGGCTG CTCTCGCCCT TCATGCCCTT TGTCACAGAG 
GAGCTATTCC AGAGACTGCC CCGGCGGACA CCAAAAGCGC CTGCTAGCCT CTGTGTCACC 
CCGTACCCAG AGCCCTCAGA GTGCTCCTGG AAGGACCCTG AAGCTGAAGC TGCTCTTGAA 
CTAGCTCTGA GCATCACTCG AGCTGTGCGC TCCCTGCGTG CTGACTACAA CCTGACCCGC 
ACCAGGCCCG ACTGTTTCTT GGAAGTAGCC GATGAGGCCA CAGGTGCCTT GGCCTCGGCA 
GTGTCGGGCT ACGTGCAGGC TCTGGCCAGT GCGGGCGTGG TGGCTGTCCT CGCCCTGGGT 
GCTCCTGCAC CGCAGGGCTG CGCTGTAGCT GTGGCTTCTG ACCGCTGCTC CATCCACCTG 
CAGCTGCAGG GGCTAGTGGA CCCAGCCCGG GAGTTGGGCA AGCTGCAGGC CAAGCGAAGT 
GAGGCACAGC GGCAGGCTCA GCGGCTGCAG GAGCGCCGTG CTGCCTCCAG CTACTCAGCA 
AAGGTGCCCC TTGAGGTCCA GGAGGCAGAT GAAGCGAAGT TGCAACAGAC AGAGGCGGAG 
CTCAGGAAGG TGGATGAGGC CATCGCCCTG TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars ( XM_017317396.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172885.1 Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79554
Clone ID Related Accession (Same CDS sequence)	XM_017317396.2 , XM_017317396.1
Accession Version	XM_017317396.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGG AGACCACCCT ATGGAACCCA GGCTGTGACC ATGCAGGCAT TGCCACCCAG 
GTGGTGGTGG AAAAGAAGCT CTGGAAAGAG CGGGGTCTGA ACCGGCACCA GCTGGGCCGC 
GAGGCCTTTC TTGAGGAGGT CTGGAAGTGG AAAGCCGAGA AGGGTGACAG GATTTACCAC 
CAATTAAAGA AACTTGGCAG CTCCTTGGAC TGGGATCGAG CCTGCTTCAC CATGGATCCT 
AAATTGTCAG CAACTGTGAC AGAGGCCTTT GTTAGGCTCC ACGAAGAAGG GGTCATCTAC 
CGTAGCACCC GCCTGGTCAA CTGGTCCTGC ACCCTCAACT CAGCCATTTC TGACATTGAG 
GTGGATAAGA AGGAGCTGAC AGGCCGCACC CTGCTTCCTG TGCCTGGCTA CAAGGAGAAG 
GTGGAGTTTG GGGTCCTTGT GTCCTTTGCC TACAAGGTCC AAGGCTCAGA CAGCGACGAG 
GAGGTGGTGG TGGCAACAAC CCGGATTGAG ACCATGCTGG GAGACGTGGC TGTAGCTGTG 
CACCCCAAGG ATCCCAGATA CCAGCACTTA AAGGGAAAGT GTGTCGTCCA CCCATTCTTG 
TCCCGGAGCC TTCCCATCGT CTTCGATGAC TTTGTAGACA TGGAGTTTGG CACAGGTGCC 
GTGAAGATCA CCCCGGCACA TGACCAAAAT GACTATGAGG TTGGGCAGCG ACACAGGCTC 
GAGGCCATTA GCATCATGGA CTCAAAGGGG GCCCTCATCA ATGTGCCTCC ACCTTTCCTG 
GGCCTGCCCA GGTTTGAGGC CAGGAAGGCT GTGCTGGCGG CACTGAAGGA GCGGGGCCTG 
TTCCGTGGAG TCAAGGACAA CCCCATGGTG GTGCCGCTTT GCAACCGCTC CAAGGATGTG 
GTGGAGCCTC TGTTAAGGCC ACAATGGTAC GTGCGCTGTG GGGAGATGGC TCAGGCTGCC 
AGTGCTGCTG TGACCCGGGG TGACCTCCGT ATACTGCCTG AGGCCCATCA ACGGACGTGG 
CATTCTTGGA TGGACAACAT CAGAGACTGG TGCATCTCTC GGCAGCTGTG GTGGGGCCAC 
CGAATCCCTG CCTACTTTAT CACCGTCCAT GACCCGGCAG TACCCCCTGG GGAGGACCCT 
GATGGGCGGT ACTGGGTAAG CGGACGCACT GAAGCAGAGG CCCGAGAGAA GGCAGCACGG 
GAGTTTGGAG TGTCCCCTGA CAAGATCAGC CTTCAGCAAG ATGAGGATGT GTTGGACACC 
TGGTTCTCCT CCGGTCTCTT TCCCTTCTCC ATCTTTGGCT GGCCCAATCA GTCAGAAGAC 
CTCAGTGTGT TCTACCCTGG GACCCTGTTG GAGACAGGCC ATGATATCCT CTTCTTCTGG 
GTGGCCCGGA TGGTCATGCT CGGCCTGAAG CTCACTGGGA AGCTGCCCTT CAGAGAGGTC 
TATCTCCATG CAATCGTGCG TGATGCTCAT GGCAGGAAGA TGAGCAAGTC TCTTGGCAAT 
GTCATCGACC CCCTGGATGT CATCCATGGA GTTTCCTTGC AGGGCCTCTA TGACCAACTA 
CTGAACAGCA ACCTGGATCC CAGTGAGGTG GAGAAAGCCA AAGAAGGACA GAAGGCCGAC 
TTTCCAGCAG GGATTCCCGA GTGTGGCACT GATGCCCTAC GCTTTGGACT CTGTGCCTAC 
ACATCCCAAG GTCGAGACAT CAACCTGGAT GTGAACAGGA TCCTGGGGTA CCGTCACTTC 
TGCAACAAAC TCTGGAATGC TACCAAGTTT GCCCTGCGCG GCCTTGGGAA GGGCTTTGTG 
CCCTCAGCAA CCTCCAAGCC TGAAGGGCAC GAGAGCCTGG TGGACCGCTG GATCCGCAGC 
CGCCTAACTG AGGCCGTGAG GCTCAGCAAT GAAGGCTTCC AGGCTTACGA TTTCCCGGCC 
ATCACCACCG CCCAGTACAG CTTTTGGCTC TATGAGCTCT GTGATGTCTA TTTGGAGTGT 
CTAAAACCTG TGCTGAATGG AGTGGACCAG GTGGCGGCAG AGTGTGCTCG GCAGACCCTC 
TACACCTGCC TGGATGTTGG CCTGCGGCTG CTCTCGCCCT TCATGCCCTT TGTCACAGAG 
GAGCTATTCC AGAGACTGCC CCGGCGGACA CCAAAAGCGC CTGCTAGCCT CTGTGTCACC 
CCGTACCCAG AGCCCTCAGA GTGCTCCTGG AAGGACCCTG AAGCTGAAGC TGCTCTTGAA 
CTAGCTCTGA GCATCACTCG AGCTGTGCGC TCCCTGCGTG CTGACTACAA CCTGACCCGC 
ACCAGGCCCG ACTGTTTCTT GGAAGTAGCC GATGAGGCCA CAGGTGCCTT GGCCTCGGCA 
GTGTCGGGCT ACGTGCAGGC TCTGGCCAGT GCGGGCGTGG TGGCTGTCCT CGCCCTGGGT 
GCTCCTGCAC CGCAGGGCTG CGCTGTAGCT GTGGCTTCTG ACCGCTGCTC CATCCACCTG 
CAGCTGCAGG GGCTAGTGGA CCCAGCCCGG GAGTTGGGCA AGCTGCAGGC CAAGCGAAGT 
GAGGCACAGC GGCAGGCTCA GCGGCTGCAG GAGCGCCGTG CTGCCTCCAG CTACTCAGCA 
AAGGTGCCCC TTGAGGTCCA GGAGGCAGAT GAAGCGAAGT TGCAACAGAC AGAGGCGGAG 
CTCAGGAAGG TGGATGAGGC CATCGCCCTG TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172885.1
CDS	462..3149
Translation	MRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGLPRFEARKAVLAALKERGLFRGVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017317396.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317396.1

ORF Insert Sequence:

ATGCGTGGGG AGACCACCCT ATGGAACCCA GGCTGTGACC ATGCAGGCAT TGCCACCCAG 
GTGGTGGTGG AAAAGAAGCT CTGGAAAGAG CGGGGTCTGA ACCGGCACCA GCTGGGCCGC 
GAGGCCTTTC TTGAGGAGGT CTGGAAGTGG AAAGCCGAGA AGGGTGACAG GATTTACCAC 
CAATTAAAGA AACTTGGCAG CTCCTTGGAC TGGGATCGAG CCTGCTTCAC CATGGATCCT 
AAATTGTCAG CAACTGTGAC AGAGGCCTTT GTTAGGCTCC ACGAAGAAGG GGTCATCTAC 
CGTAGCACCC GCCTGGTCAA CTGGTCCTGC ACCCTCAACT CAGCCATTTC TGACATTGAG 
GTGGATAAGA AGGAGCTGAC AGGCCGCACC CTGCTTCCTG TGCCTGGCTA CAAGGAGAAG 
GTGGAGTTTG GGGTCCTTGT GTCCTTTGCC TACAAGGTCC AAGGCTCAGA CAGCGACGAG 
GAGGTGGTGG TGGCAACAAC CCGGATTGAG ACCATGCTGG GAGACGTGGC TGTAGCTGTG 
CACCCCAAGG ATCCCAGATA CCAGCACTTA AAGGGAAAGT GTGTCGTCCA CCCATTCTTG 
TCCCGGAGCC TTCCCATCGT CTTCGATGAC TTTGTAGACA TGGAGTTTGG CACAGGTGCC 
GTGAAGATCA CCCCGGCACA TGACCAAAAT GACTATGAGG TTGGGCAGCG ACACAGGCTC 
GAGGCCATTA GCATCATGGA CTCAAAGGGG GCCCTCATCA ATGTGCCTCC ACCTTTCCTG 
GGCCTGCCCA GGTTTGAGGC CAGGAAGGCT GTGCTGGCGG CACTGAAGGA GCGGGGCCTG 
TTCCGTGGAG TCAAGGACAA CCCCATGGTG GTGCCGCTTT GCAACCGCTC CAAGGATGTG 
GTGGAGCCTC TGTTAAGGCC ACAATGGTAC GTGCGCTGTG GGGAGATGGC TCAGGCTGCC 
AGTGCTGCTG TGACCCGGGG TGACCTCCGT ATACTGCCTG AGGCCCATCA ACGGACGTGG 
CATTCTTGGA TGGACAACAT CAGAGACTGG TGCATCTCTC GGCAGCTGTG GTGGGGCCAC 
CGAATCCCTG CCTACTTTAT CACCGTCCAT GACCCGGCAG TACCCCCTGG GGAGGACCCT 
GATGGGCGGT ACTGGGTAAG CGGACGCACT GAAGCAGAGG CCCGAGAGAA GGCAGCACGG 
GAGTTTGGAG TGTCCCCTGA CAAGATCAGC CTTCAGCAAG ATGAGGATGT GTTGGACACC 
TGGTTCTCCT CCGGTCTCTT TCCCTTCTCC ATCTTTGGCT GGCCCAATCA GTCAGAAGAC 
CTCAGTGTGT TCTACCCTGG GACCCTGTTG GAGACAGGCC ATGATATCCT CTTCTTCTGG 
GTGGCCCGGA TGGTCATGCT CGGCCTGAAG CTCACTGGGA AGCTGCCCTT CAGAGAGGTC 
TATCTCCATG CAATCGTGCG TGATGCTCAT GGCAGGAAGA TGAGCAAGTC TCTTGGCAAT 
GTCATCGACC CCCTGGATGT CATCCATGGA GTTTCCTTGC AGGGCCTCTA TGACCAACTA 
CTGAACAGCA ACCTGGATCC CAGTGAGGTG GAGAAAGCCA AAGAAGGACA GAAGGCCGAC 
TTTCCAGCAG GGATTCCCGA GTGTGGCACT GATGCCCTAC GCTTTGGACT CTGTGCCTAC 
ACATCCCAAG GTCGAGACAT CAACCTGGAT GTGAACAGGA TCCTGGGGTA CCGTCACTTC 
TGCAACAAAC TCTGGAATGC TACCAAGTTT GCCCTGCGCG GCCTTGGGAA GGGCTTTGTG 
CCCTCAGCAA CCTCCAAGCC TGAAGGGCAC GAGAGCCTGG TGGACCGCTG GATCCGCAGC 
CGCCTAACTG AGGCCGTGAG GCTCAGCAAT GAAGGCTTCC AGGCTTACGA TTTCCCGGCC 
ATCACCACCG CCCAGTACAG CTTTTGGCTC TATGAGCTCT GTGATGTCTA TTTGGAGTGT 
CTAAAACCTG TGCTGAATGG AGTGGACCAG GTGGCGGCAG AGTGTGCTCG GCAGACCCTC 
TACACCTGCC TGGATGTTGG CCTGCGGCTG CTCTCGCCCT TCATGCCCTT TGTCACAGAG 
GAGCTATTCC AGAGACTGCC CCGGCGGACA CCAAAAGCGC CTGCTAGCCT CTGTGTCACC 
CCGTACCCAG AGCCCTCAGA GTGCTCCTGG AAGGACCCTG AAGCTGAAGC TGCTCTTGAA 
CTAGCTCTGA GCATCACTCG AGCTGTGCGC TCCCTGCGTG CTGACTACAA CCTGACCCGC 
ACCAGGCCCG ACTGTTTCTT GGAAGTAGCC GATGAGGCCA CAGGTGCCTT GGCCTCGGCA 
GTGTCGGGCT ACGTGCAGGC TCTGGCCAGT GCGGGCGTGG TGGCTGTCCT CGCCCTGGGT 
GCTCCTGCAC CGCAGGGCTG CGCTGTAGCT GTGGCTTCTG ACCGCTGCTC CATCCACCTG 
CAGCTGCAGG GGCTAGTGGA CCCAGCCCGG GAGTTGGGCA AGCTGCAGGC CAAGCGAAGT 
GAGGCACAGC GGCAGGCTCA GCGGCTGCAG GAGCGCCGTG CTGCCTCCAG CTACTCAGCA 
AAGGTGCCCC TTGAGGTCCA GGAGGCAGAT GAAGCGAAGT TGCAACAGAC AGAGGCGGAG 
CTCAGGAAGG TGGATGAGGC CATCGCCCTG TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars ( NM_011690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035820.3 Mus musculus valyl-tRNA synthetase (Vars), mRNA.

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CloneID	OMu13227
Clone ID Related Accession (Same CDS sequence)	NM_011690.3 , XM_017317395.2 , XM_017317395.1
Accession Version	NM_011690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ080276.1, BY775300.1, AK157814.1, AK019157.1 and BB070705.1. On Aug 4, 2009 this sequence version replaced NM_011690.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154823.1, BC053703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035820.3
CDS	359..4150
Translation	MSILYVSPHPDAFPSLRALIAARYGEAGDGPGWGGPHPRICLQPPPSSRTPFPPPRLPALEQGPGGLWVWGAPAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRGPGQDPQAALGALGKALNPLEDWLRLHTYLAGDAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFNTCVRQPEFRAVLGEVALYSGARSVTQQPGSEVIAPQKTPAQLKKEAKKREKLEKFQQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVSGAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGLPRFEARKAVLAALKERGLFRGVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	NM_011690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase (Vars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011690.3

ORF Insert Sequence:

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars ( XM_017317395.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172884.1 Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

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CloneID	OMu13227
Clone ID Related Accession (Same CDS sequence)	NM_011690.3 , XM_017317395.2 , XM_017317395.1
Accession Version	XM_017317395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172884.1
CDS	182..3973
Translation	MSILYVSPHPDAFPSLRALIAARYGEAGDGPGWGGPHPRICLQPPPSSRTPFPPPRLPALEQGPGGLWVWGAPAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRGPGQDPQAALGALGKALNPLEDWLRLHTYLAGDAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFNTCVRQPEFRAVLGEVALYSGARSVTQQPGSEVIAPQKTPAQLKKEAKKREKLEKFQQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVSGAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGLPRFEARKAVLAALKERGLFRGVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017317395.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317395.2

ORF Insert Sequence:

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vars ( XM_017317395.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172884.1 Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

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CloneID	OMu13227
Clone ID Related Accession (Same CDS sequence)	NM_011690.3 , XM_017317395.2 , XM_017317395.1
Accession Version	XM_017317395.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	valine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172884.1
CDS	144..3935
Translation	MSILYVSPHPDAFPSLRALIAARYGEAGDGPGWGGPHPRICLQPPPSSRTPFPPPRLPALEQGPGGLWVWGAPAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRGPGQDPQAALGALGKALNPLEDWLRLHTYLAGDAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFNTCVRQPEFRAVLGEVALYSGARSVTQQPGSEVIAPQKTPAQLKKEAKKREKLEKFQQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVSGAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLEEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALINVPPPFLGLPRFEARKAVLAALKERGLFRGVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYDQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDFPAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017317395.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus valyl-tRNA synthetase (Vars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317395.1

ORF Insert Sequence:

ATGTCCATCC TCTACGTCTC TCCTCACCCC GACGCCTTCC CCAGCCTCCG AGCCCTCATC 
GCTGCCCGAT ACGGGGAAGC TGGTGACGGT CCCGGGTGGG GAGGCCCTCA CCCCCGCATC 
TGCTTGCAGC CTCCCCCGAG CAGCCGGACT CCATTCCCTC CGCCTCGCCT GCCCGCCCTG 
GAGCAGGGAC CTGGCGGCCT GTGGGTGTGG GGGGCCCCGG CTGTAGCTCA GCTGTTGTGG 
CCTGCAGGCC TGGGGGGACC CGGGGGCAGC CGGGCAGCTG TCCTGGTCCA GCAGTGGGTC 
AGTTATGCTG ACACGGAGCT CATACCCGCT GCCTGCGGGG CGACCCTGCC TGCCCTGGGA 
CTTCGAGGTC CTGGTCAGGA TCCCCAGGCT GCCCTTGGGG CCCTGGGCAA GGCCTTGAAC 
CCCTTGGAGG ACTGGCTGCG GCTGCACACC TACCTGGCTG GAGATGCCCC CACTCTGGCT 
GACTTAGCTG CTGTGACAGC CTTACTGCTG CCTTTCCGAT ACGTTCTGGA CCCTTCTGCC 
CGCCGGATCT GGGGCAATGT GACTCGCTGG TTTAACACTT GTGTCCGGCA ACCGGAATTC 
CGGGCTGTGC TGGGAGAAGT GGCCCTGTAC TCGGGGGCCA GGTCTGTCAC TCAACAGCCA 
GGCTCTGAGG TCATCGCACC CCAAAAAACA CCCGCGCAGC TCAAAAAAGA GGCAAAGAAA 
CGGGAGAAAC TAGAGAAATT CCAGCAGAAG CAGAAGACCC AGCAGCAGCC CCCGCACGGA 
GAGAAGAAAC CAAAACCAGA GAAGAAGGAG AAACGGGACC CTGGGGTCAT TACCTATGAC 
CTCCCTACCC CACCGGGGGA GAAGAAAGAT GTAAGTGGCG CCATGCCCGA CTCCTACAGC 
CCTCAGTATG TGGAGGCTGC CTGGTATCCA TGGTGGGAGC GGCAGGGCTT CTTCAAGCCA 
GAGTACGGGC GTCCTAGTGT GTCAGCACCA AATCCCCGGG GTGTCTTCAT GATGTGCATC 
CCACCCCCCA ACGTGACAGG CTCCCTGCAC CTGGGCCACG CACTCACCAA CGCCATCCAG 
GACTCCCTGA CTCGATGGCA CCGCATGCGT GGGGAGACCA CCCTATGGAA CCCAGGCTGT 
GACCATGCAG GCATTGCCAC CCAGGTGGTG GTGGAAAAGA AGCTCTGGAA AGAGCGGGGT 
CTGAACCGGC ACCAGCTGGG CCGCGAGGCC TTTCTTGAGG AGGTCTGGAA GTGGAAAGCC 
GAGAAGGGTG ACAGGATTTA CCACCAATTA AAGAAACTTG GCAGCTCCTT GGACTGGGAT 
CGAGCCTGCT TCACCATGGA TCCTAAATTG TCAGCAACTG TGACAGAGGC CTTTGTTAGG 
CTCCACGAAG AAGGGGTCAT CTACCGTAGC ACCCGCCTGG TCAACTGGTC CTGCACCCTC 
AACTCAGCCA TTTCTGACAT TGAGGTGGAT AAGAAGGAGC TGACAGGCCG CACCCTGCTT 
CCTGTGCCTG GCTACAAGGA GAAGGTGGAG TTTGGGGTCC TTGTGTCCTT TGCCTACAAG 
GTCCAAGGCT CAGACAGCGA CGAGGAGGTG GTGGTGGCAA CAACCCGGAT TGAGACCATG 
CTGGGAGACG TGGCTGTAGC TGTGCACCCC AAGGATCCCA GATACCAGCA CTTAAAGGGA 
AAGTGTGTCG TCCACCCATT CTTGTCCCGG AGCCTTCCCA TCGTCTTCGA TGACTTTGTA 
GACATGGAGT TTGGCACAGG TGCCGTGAAG ATCACCCCGG CACATGACCA AAATGACTAT 
GAGGTTGGGC AGCGACACAG GCTCGAGGCC ATTAGCATCA TGGACTCAAA GGGGGCCCTC 
ATCAATGTGC CTCCACCTTT CCTGGGCCTG CCCAGGTTTG AGGCCAGGAA GGCTGTGCTG 
GCGGCACTGA AGGAGCGGGG CCTGTTCCGT GGAGTCAAGG ACAACCCCAT GGTGGTGCCG 
CTTTGCAACC GCTCCAAGGA TGTGGTGGAG CCTCTGTTAA GGCCACAATG GTACGTGCGC 
TGTGGGGAGA TGGCTCAGGC TGCCAGTGCT GCTGTGACCC GGGGTGACCT CCGTATACTG 
CCTGAGGCCC ATCAACGGAC GTGGCATTCT TGGATGGACA ACATCAGAGA CTGGTGCATC 
TCTCGGCAGC TGTGGTGGGG CCACCGAATC CCTGCCTACT TTATCACCGT CCATGACCCG 
GCAGTACCCC CTGGGGAGGA CCCTGATGGG CGGTACTGGG TAAGCGGACG CACTGAAGCA 
GAGGCCCGAG AGAAGGCAGC ACGGGAGTTT GGAGTGTCCC CTGACAAGAT CAGCCTTCAG 
CAAGATGAGG ATGTGTTGGA CACCTGGTTC TCCTCCGGTC TCTTTCCCTT CTCCATCTTT 
GGCTGGCCCA ATCAGTCAGA AGACCTCAGT GTGTTCTACC CTGGGACCCT GTTGGAGACA 
GGCCATGATA TCCTCTTCTT CTGGGTGGCC CGGATGGTCA TGCTCGGCCT GAAGCTCACT 
GGGAAGCTGC CCTTCAGAGA GGTCTATCTC CATGCAATCG TGCGTGATGC TCATGGCAGG 
AAGATGAGCA AGTCTCTTGG CAATGTCATC GACCCCCTGG ATGTCATCCA TGGAGTTTCC 
TTGCAGGGCC TCTATGACCA ACTACTGAAC AGCAACCTGG ATCCCAGTGA GGTGGAGAAA 
GCCAAAGAAG GACAGAAGGC CGACTTTCCA GCAGGGATTC CCGAGTGTGG CACTGATGCC 
CTACGCTTTG GACTCTGTGC CTACACATCC CAAGGTCGAG ACATCAACCT GGATGTGAAC 
AGGATCCTGG GGTACCGTCA CTTCTGCAAC AAACTCTGGA ATGCTACCAA GTTTGCCCTG 
CGCGGCCTTG GGAAGGGCTT TGTGCCCTCA GCAACCTCCA AGCCTGAAGG GCACGAGAGC 
CTGGTGGACC GCTGGATCCG CAGCCGCCTA ACTGAGGCCG TGAGGCTCAG CAATGAAGGC 
TTCCAGGCTT ACGATTTCCC GGCCATCACC ACCGCCCAGT ACAGCTTTTG GCTCTATGAG 
CTCTGTGATG TCTATTTGGA GTGTCTAAAA CCTGTGCTGA ATGGAGTGGA CCAGGTGGCG 
GCAGAGTGTG CTCGGCAGAC CCTCTACACC TGCCTGGATG TTGGCCTGCG GCTGCTCTCG 
CCCTTCATGC CCTTTGTCAC AGAGGAGCTA TTCCAGAGAC TGCCCCGGCG GACACCAAAA 
GCGCCTGCTA GCCTCTGTGT CACCCCGTAC CCAGAGCCCT CAGAGTGCTC CTGGAAGGAC 
CCTGAAGCTG AAGCTGCTCT TGAACTAGCT CTGAGCATCA CTCGAGCTGT GCGCTCCCTG 
CGTGCTGACT ACAACCTGAC CCGCACCAGG CCCGACTGTT TCTTGGAAGT AGCCGATGAG 
GCCACAGGTG CCTTGGCCTC GGCAGTGTCG GGCTACGTGC AGGCTCTGGC CAGTGCGGGC 
GTGGTGGCTG TCCTCGCCCT GGGTGCTCCT GCACCGCAGG GCTGCGCTGT AGCTGTGGCT 
TCTGACCGCT GCTCCATCCA CCTGCAGCTG CAGGGGCTAG TGGACCCAGC CCGGGAGTTG 
GGCAAGCTGC AGGCCAAGCG AAGTGAGGCA CAGCGGCAGG CTCAGCGGCT GCAGGAGCGC 
CGTGCTGCCT CCAGCTACTC AGCAAAGGTG CCCCTTGAGG TCCAGGAGGC AGATGAAGCG 
AAGTTGCAAC AGACAGAGGC GGAGCTCAGG AAGGTGGATG AGGCCATCGC CCTGTTCCAG 
AAGATGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Genome research13(12)2621-36(2003 Dec)
Xie T,Rowen L,Aguado B,Ahearn ME,Madan A,Qin S,Campbell RD,Hood L

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Genome research13(6B)1273-89(2003 Jun)
Carninci P,Waki K,Shiraki T,Konno H,Shibata K,Itoh M,Aizawa K,Arakawa T,Ishii Y,Sasaki D,Bono H,Kondo S,Sugahara Y,Saito R,Osato N,Fukuda S,Sato K,Watahiki A,Hirozane-Kishikawa T,Nakamura M,Shibata Y,Yasunishi A,Kikuchi N,Yoshiki A,Kusakabe M,Gustincich S,Beisel K,Pavan W,Aidinis V,Nakagawara A,Held WA,Iwata H,Kono T,Nakauchi H,Lyons P,Wells C,Hume DA,Fagiolini M,Hensch TK,Brinkmeier M,Camper S,Hirota J,Mombaerts P,Muramatsu M,Okazaki Y,Kawai J,Hayashizaki Y

Genotype versus phenotype: conflicting results in mapping a lung tumor susceptibility locus to the G7c recombination interval in the mouse MHC class III region.
Immunogenetics53(8)656-61(2001 Oct)
van Kooij M,de Groot K,van Vugt H,Aten J,Snoek M

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Vars cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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