Polrmt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polrmt
Entrez Gene ID	216151
Full Name	polymerase (RNA) mitochondrial (DNA directed)
Synonyms	1110018N15Rik,4932416K13
General protein information	Preferred Names polymerase (RNA) mitochondrial (DNA directed) Names DNA-directed RNA polymerase, mitochondrial mitochondrial DNA-directed RNA polymerase mtRPOL
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_172551.3	NP_766139.2	DNA-directed RNA polymerase, mitochondrial precursor
XM_030245018.1	XP_030100878.1	DNA-directed RNA polymerase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	polrmt	XP_004910992.1
Oryza sativa (rice)	Os06g0652000	NP_001058231.2
Canis lupus familiaris (dog)	POLRMT	XP_005633227.1
Gallus gallus (chicken)	POLRMT	XP_418204.3
Arabidopsis thaliana (thale cress)	SCA3	NP_179989.1
Mus musculus (house mouse)	Polrmt	NP_766139.2
Pan troglodytes (chimpanzee)	POLRMT	XP_003316023.1
Bos taurus (cattle)	POLRMT	NP_001192480.1
Danio rerio (zebrafish)	si:dkey-21e2.1	XP_005170578.1
Homo sapiens (human)	POLRMT	NP_005026.3
Rattus norvegicus (Norway rat)	Polrmt	NP_001100236.1
Drosophila melanogaster (fruit fly)	mtRNApol	NP_001259853.1

Gene Ontology
Bibliography

Related articles in PubMed

TEFM regulates both transcription elongation and RNA processing in mitochondria.
Jiang S, Koolmeister C, Misic J, Siira S, Kühl I, Silva Ramos E, Miranda M, Jiang M, Posse V, Lytovchenko O, Atanassov I, Schober FA, Wibom R, Hultenby K, Milenkovic D, Gustafsson CM, Filipovska A, Larsson NG
EMBO reports20(6)(2019 06)

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
Kühl I, Miranda M, Atanassov I, Kuznetsova I, Hinze Y, Mourier A, Filipovska A, Larsson NG
eLife6(2017 11)

POLRMT regulates the switch between replication primer formation and gene expression of mammalian mtDNA.
Kühl I, Miranda M, Posse V, Milenkovic D, Mourier A, Siira SJ, Bonekamp NA, Neumann U, Filipovska A, Polosa PL, Gustafsson CM, Larsson NG
Science advances2(8)e1600963(2016 08)

POLRMT does not transcribe nuclear genes.
Kühl I, Kukat C, Ruzzenente B, Milenkovic D, Mourier A, Miranda M, Koolmeister C, Falkenberg M, Larsson NG
Nature514(7521)E7-11(2014 Oct)

A novel AMPK-dependent FoxO3A-SIRT3 intramitochondrial complex sensing glucose levels.
Peserico A, Chiacchiera F, Grossi V, Matrone A, Latorre D, Simonatto M, Fusella A, Ryall JG, Finley LW, Haigis MC, Villani G, Puri PL, Sartorelli V, Simone C
Cellular and molecular life sciences : CMLS70(11)2015-29(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polrmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polrmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu85415	XM_030245018.1 Latest version!	Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13377	NM_172551.3 Latest version!	Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$713.30 $1019.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polrmt ( XM_030245018.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100878.1 Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu85415
Clone ID Related Accession (Same CDS sequence)	XM_030245018.1
Accession Version	XM_030245018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGC TCCGGTGGAC CCGAAGCGCA GCCGGGCTTG GCCGGGTTCT GCGCTCCCCT 
GGGCCCCACC GCCCGCCTTC GGAGGAAGGG ACTTTTGGTG GCTTCTGCAG CTCAAGAAGG 
AGCTCCGCTG CCAGTCCCCG TGAGCAACAT GTCCTGAGGG AGTGGGGCCA TGCTGAACTG 
CTGGAAGTGC TGGAGGCTCG GGTGCGGCAG CTCCGGGCAG AGGGCACACC TGAGATGAGG 
GTGAAGAAGG TGCAGGTGGA CCGGCCTCCA CAGGGCCACA GCAGCCGCTG GGCGCAAAAG 
CTAGAGGCTG AGAAAAGGGT GAAGCAGAGG CGCCAAAAGG AAGTTGACCA GCAGAAGCAA 
GCCCTCACAC AGGAGTTCTG GACCCTTCAC AAGGAGCCCA AGATCTGGAA CAAGAAGCTG 
GCTGGCTACC TGCAGCCAAG CAAGAAGGGA ACACCCACGA ACTCAGAGGA AAAGCAGCTG 
GCCCAGGCCC TTCAGGCTGC TCTGGGGAGG CTCAGCTCCC GTGAGGCAGA GGCCCTGGCC 
AGGAAGAAAG CCAAGGCGGT GGAGGCGCAG ATCCTGGTCC TCCAGCAGAA GTTCCTGGCT 
TTCTTTGAGT GCTGCGTCTG CACTGGCCAA GTGCCCCTCG CTCACCACGT GCTGGTCACT 
CACCATAACA ACGGAGACAG ACAGCAGGTG CTCACACTGC ACATGTACAA CACCGTGATG 
CTTGGCTGGG CCCGCAAGGG CTCCTTCAGA GAGCTGGTCT ATGTGTTCCT CATGCTGAAG 
GATGCTGGCC TCTCCCCAGA CCTGTGCTCC TATGCAGCTG CACTCCAGTG CATGGGACGC 
AGGGACCAGG ATGTTCGCAC CATCCAGAGG TGTCTGAAGC AGATGATGGA AGAAGGCTTC 
CAGCCCCAGC TGCTCTTCAC TGACCTGGTC CTGGAAGAGG AGGACCGGGC CGCGCTCCTG 
AGAGCAGTGG TCAAGGCTGA GCCTGCCTTT CGTCCACCGC CACAGGCCCC AAGCCCAGTT 
AACACATCCA CGCTGCTCAA GGACATATAT AGCAAGGAGG GCCCTGTGTC CTACCCAAAG 
CTGCACCTGC CCCTGGACAC CCTGCAGGAC CTCTTCTACC AGCAGCTGCA TGTGGAGCTG 
AGCTCCAGTG TCTGTGTCCA GTCGGTGGAG AAGGCTCCAG TAATGTCCAA GGAGGTCATT 
GAGGCGCGGA AGACCCTGCA GGCCCTGCGG GAGCAGTGGG AAGTGGAGCT GTTGCGCGTG 
CTGCGGGAGA CAAAGGCCAC TATGGGCCGC CAGGCCTATG AAGGCCAGCC CACCCTCTAC 
CCGTTCCTGT GCCTGCTGAG CGAAGGGGAG TTTGTGAGCA TACTGATGCA GGTTCTGAAG 
GTCCTGCCTG CGCAGGGTGA GCCCCTTATC CAGTTGGCCC ATAACCTGGG CCTTCGGGTG 
TTAAACCGGC ATTTGGTGAA GCAGAAGCAG GTCACTAACC ATGTGCAGAA GCTGGGGCAG 
CGGTACTCGC AGTACCTCCA GCTGCTGGCG TCTGACACCC AGGTGGCGCC CTGCCTTCCT 
CGGGAGTACT GGGAATCGCT GGGCCCACTG GAGGCCCCTG CACAACAGCC CTGGTCCGTG 
CCAGTGCTGC TACAGCTGGG CAAGCAATTG GCGGAGCTGC TGGTGCAGGC AGTGCAGATG 
CCGCGCAGCC TGGCGGCCCG GCAGGGTGCT CAGCGCTCCA TCCCAGTGCT TTACCATGTG 
TATTCCTTCC GAAGCTACCG CCAGGTGGGC ATCCTTAAGC CTCACCCTGC CTTCACGCAC 
CTGCTAGAGA CGGCAGCAGA GCCCACACTG ACCTTCGAGA CCACGGAAGT GCCCATGCTG 
TGCCCACCAC TGCCCTGGAC GTCGCTGCAT TCTGGTGCCT ACTTGCTGAG CTCCACCAAA 
CTAATGCGTG CCACGGAGGG TACCACACAG CACCAGCGTC TGCTGGAGCA ATGCCCTCCT 
GCCCAGCTGC ACGGCCCCCT GGATGCGCTC ACACAGTTGG GGAACTGCGC CTGGCGTGTA 
AACGGGCATC TGCTGGACTT GGTGCTGCAG ATCTTTAGAG ACAAGGGCTG TATGCCCTTG 
GGCGTGCCTC CCCCGCGCTC AGAAGCACCG CGGCCGGCCC GGTATCAGCT GCCACCCGGC 
TCCACACCAG TGCACAAGTC TGAGCTGCGG AAGGAGCTGG CGCGTTGCCT CAAGGTGGCT 
CGAGAGATGC ACAGTCTGCG CAGCGAGGCC CTGTATCGCC TGTCGCTGGC ACAGCACCTA 
CGCCACCGTG TCTTCTGGTT GCCGCACAAC ATGGACTTCC GCGGCCGCAC TTACCCCTGC 
CCGCCACACT TCAACCACCT GGGCAGTGAC CTAGCGCGTG CGCTATTGGA GTTTGCTGAG 
GGCCGGCCAC TGGGACCACG TGGTCTGGAC TGGCTTAAGA TCCACCTGAT CAACCTGACT 
GGCCTCAAGA AGGGAGACTC GCTGCGCATG CGCCTAGCCT TCGCAGATGA GGTCATGGAG 
GAGATCTTGG ACTCTGCAGA CAACCCCTTG ACGGGCCGGA AGTGGTGGAT GGAAGCTGAT 
GAGCCCTGGC AGACCCTGGC CTGCTGCATG GAGGTGGCAC ACGCAGTCCG GTCCCCAGAC 
CCTGCTGCCT ACATTTCCCA CCTGCCAGTT CACCAGGATG GCTCCTGCAA TGGCTTACAG 
CATTACGCCG CACTAGGCCG AGACAGTGTG GGTGCTGCCT CAGTCAACCT AACGCCGTCC 
GACCTGCCCC AAGATGTGTA CAGGGAGGTG GCAACACAGG TGGAGGAGTT CCGCCAGCAG 
GACGCCAAGG AGGGTCTACG GGTGGCTCAG GTGCTGGAGG GCTTCATCAG CCGCAAGGTG 
GTGAAGCAGA CAGTGATGAC GGTGGTGTAT GGGGTCACAC GCTACGGAGG GCGCCTGCAG 
ATAGAGAAGC GCCTGCGCGA ACTCAGCGAC TTCCCTCAGG AGTTTGTCTG GGAAGCCTCA 
CACTACCTCG TGCGCCAGGT CTTCAAAAGT CTACAGGAGA TGTTCACCAG CACGCGGGCC 
ATTCAGCACT GGCTGACTGA GAGTGCCAAC CTCATCTCTC ACGCCGGATG GCCTGTGGAG 
TGGGTCACGC CCCTGGGCAT CCCCATCATA CAGCCCTATC ACCGCGAGTC CAAGGTCCAG 
GTACTTGGGA AATGCCCTTC TCCTTTTCCC TCATCTCCCC TCCTCCCTGC CAAGGGCTGG 
ACTCACCCCT CCTCCAACCC CGGTCTGCAG GTAAAAGGTG GCCTCCAGAG CATCACCCTC 
ACCAGCTCGG TAGATGAGAG TCAGAAGCCC AACACTCTGA AGCAGAAAAA TGGCTTCCCG 
CCCAACTTCA TCCACTCCCT GGACTCCTCC CACATGATGC TGACCGCCCT ACACTGCTAC 
AGGAAGGGCC TGATTTTCGT CTCCGTGCAC GACTGCTTCT GGACACATGC CGCTGACATC 
CCCACGATGA ACGAGGTATG CCGCGAGCAA TTCGTGCGCC TGCACAGCCA GCCCATCCTG 
GAAGACCTGG CCAAGTTCCT GAAGAAGCGT TTCTGCTCCG TGTCCAGCAT CAAGTCCCTA 
AAGTCCTCGG AGCGCGCCCT GGTCACCAAG CTGCAGGAGA CACTACAGTC CTTGCCAAAG 
ACAGGTACCT TCGATCTAGG ACAGGTGATA AGATCCACCT ACTTTTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100878.1
CDS	53..3766
Translation	MSALRWTRSAAGLGRVLRSPGPHRPPSEEGTFGGFCSSRRSSAASPREQHVLREWGHAELLEVLEARVRQLRAEGTPEMRVKKVQVDRPPQGHSSRWAQKLEAEKRVKQRRQKEVDQQKQALTQEFWTLHKEPKIWNKKLAGYLQPSKKGTPTNSEEKQLAQALQAALGRLSSREAEALARKKAKAVEAQILVLQQKFLAFFECCVCTGQVPLAHHVLVTHHNNGDRQQVLTLHMYNTVMLGWARKGSFRELVYVFLMLKDAGLSPDLCSYAAALQCMGRRDQDVRTIQRCLKQMMEEGFQPQLLFTDLVLEEEDRAALLRAVVKAEPAFRPPPQAPSPVNTSTLLKDIYSKEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVCVQSVEKAPVMSKEVIEARKTLQALREQWEVELLRVLRETKATMGRQAYEGQPTLYPFLCLLSEGEFVSILMQVLKVLPAQGEPLIQLAHNLGLRVLNRHLVKQKQVTNHVQKLGQRYSQYLQLLASDTQVAPCLPREYWESLGPLEAPAQQPWSVPVLLQLGKQLAELLVQAVQMPRSLAARQGAQRSIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPMLCPPLPWTSLHSGAYLLSSTKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELRKELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVRSPDPAAYISHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTESANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVLGKCPSPFPSSPLLPAKGWTHPSSNPGLQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS

Target ORF information:

RefSeq Version	XM_030245018.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245018.1

ORF Insert Sequence:

ATGTCGGCGC TCCGGTGGAC CCGAAGCGCA GCCGGGCTTG GCCGGGTTCT GCGCTCCCCT 
GGGCCCCACC GCCCGCCTTC GGAGGAAGGG ACTTTTGGTG GCTTCTGCAG CTCAAGAAGG 
AGCTCCGCTG CCAGTCCCCG TGAGCAACAT GTCCTGAGGG AGTGGGGCCA TGCTGAACTG 
CTGGAAGTGC TGGAGGCTCG GGTGCGGCAG CTCCGGGCAG AGGGCACACC TGAGATGAGG 
GTGAAGAAGG TGCAGGTGGA CCGGCCTCCA CAGGGCCACA GCAGCCGCTG GGCGCAAAAG 
CTAGAGGCTG AGAAAAGGGT GAAGCAGAGG CGCCAAAAGG AAGTTGACCA GCAGAAGCAA 
GCCCTCACAC AGGAGTTCTG GACCCTTCAC AAGGAGCCCA AGATCTGGAA CAAGAAGCTG 
GCTGGCTACC TGCAGCCAAG CAAGAAGGGA ACACCCACGA ACTCAGAGGA AAAGCAGCTG 
GCCCAGGCCC TTCAGGCTGC TCTGGGGAGG CTCAGCTCCC GTGAGGCAGA GGCCCTGGCC 
AGGAAGAAAG CCAAGGCGGT GGAGGCGCAG ATCCTGGTCC TCCAGCAGAA GTTCCTGGCT 
TTCTTTGAGT GCTGCGTCTG CACTGGCCAA GTGCCCCTCG CTCACCACGT GCTGGTCACT 
CACCATAACA ACGGAGACAG ACAGCAGGTG CTCACACTGC ACATGTACAA CACCGTGATG 
CTTGGCTGGG CCCGCAAGGG CTCCTTCAGA GAGCTGGTCT ATGTGTTCCT CATGCTGAAG 
GATGCTGGCC TCTCCCCAGA CCTGTGCTCC TATGCAGCTG CACTCCAGTG CATGGGACGC 
AGGGACCAGG ATGTTCGCAC CATCCAGAGG TGTCTGAAGC AGATGATGGA AGAAGGCTTC 
CAGCCCCAGC TGCTCTTCAC TGACCTGGTC CTGGAAGAGG AGGACCGGGC CGCGCTCCTG 
AGAGCAGTGG TCAAGGCTGA GCCTGCCTTT CGTCCACCGC CACAGGCCCC AAGCCCAGTT 
AACACATCCA CGCTGCTCAA GGACATATAT AGCAAGGAGG GCCCTGTGTC CTACCCAAAG 
CTGCACCTGC CCCTGGACAC CCTGCAGGAC CTCTTCTACC AGCAGCTGCA TGTGGAGCTG 
AGCTCCAGTG TCTGTGTCCA GTCGGTGGAG AAGGCTCCAG TAATGTCCAA GGAGGTCATT 
GAGGCGCGGA AGACCCTGCA GGCCCTGCGG GAGCAGTGGG AAGTGGAGCT GTTGCGCGTG 
CTGCGGGAGA CAAAGGCCAC TATGGGCCGC CAGGCCTATG AAGGCCAGCC CACCCTCTAC 
CCGTTCCTGT GCCTGCTGAG CGAAGGGGAG TTTGTGAGCA TACTGATGCA GGTTCTGAAG 
GTCCTGCCTG CGCAGGGTGA GCCCCTTATC CAGTTGGCCC ATAACCTGGG CCTTCGGGTG 
TTAAACCGGC ATTTGGTGAA GCAGAAGCAG GTCACTAACC ATGTGCAGAA GCTGGGGCAG 
CGGTACTCGC AGTACCTCCA GCTGCTGGCG TCTGACACCC AGGTGGCGCC CTGCCTTCCT 
CGGGAGTACT GGGAATCGCT GGGCCCACTG GAGGCCCCTG CACAACAGCC CTGGTCCGTG 
CCAGTGCTGC TACAGCTGGG CAAGCAATTG GCGGAGCTGC TGGTGCAGGC AGTGCAGATG 
CCGCGCAGCC TGGCGGCCCG GCAGGGTGCT CAGCGCTCCA TCCCAGTGCT TTACCATGTG 
TATTCCTTCC GAAGCTACCG CCAGGTGGGC ATCCTTAAGC CTCACCCTGC CTTCACGCAC 
CTGCTAGAGA CGGCAGCAGA GCCCACACTG ACCTTCGAGA CCACGGAAGT GCCCATGCTG 
TGCCCACCAC TGCCCTGGAC GTCGCTGCAT TCTGGTGCCT ACTTGCTGAG CTCCACCAAA 
CTAATGCGTG CCACGGAGGG TACCACACAG CACCAGCGTC TGCTGGAGCA ATGCCCTCCT 
GCCCAGCTGC ACGGCCCCCT GGATGCGCTC ACACAGTTGG GGAACTGCGC CTGGCGTGTA 
AACGGGCATC TGCTGGACTT GGTGCTGCAG ATCTTTAGAG ACAAGGGCTG TATGCCCTTG 
GGCGTGCCTC CCCCGCGCTC AGAAGCACCG CGGCCGGCCC GGTATCAGCT GCCACCCGGC 
TCCACACCAG TGCACAAGTC TGAGCTGCGG AAGGAGCTGG CGCGTTGCCT CAAGGTGGCT 
CGAGAGATGC ACAGTCTGCG CAGCGAGGCC CTGTATCGCC TGTCGCTGGC ACAGCACCTA 
CGCCACCGTG TCTTCTGGTT GCCGCACAAC ATGGACTTCC GCGGCCGCAC TTACCCCTGC 
CCGCCACACT TCAACCACCT GGGCAGTGAC CTAGCGCGTG CGCTATTGGA GTTTGCTGAG 
GGCCGGCCAC TGGGACCACG TGGTCTGGAC TGGCTTAAGA TCCACCTGAT CAACCTGACT 
GGCCTCAAGA AGGGAGACTC GCTGCGCATG CGCCTAGCCT TCGCAGATGA GGTCATGGAG 
GAGATCTTGG ACTCTGCAGA CAACCCCTTG ACGGGCCGGA AGTGGTGGAT GGAAGCTGAT 
GAGCCCTGGC AGACCCTGGC CTGCTGCATG GAGGTGGCAC ACGCAGTCCG GTCCCCAGAC 
CCTGCTGCCT ACATTTCCCA CCTGCCAGTT CACCAGGATG GCTCCTGCAA TGGCTTACAG 
CATTACGCCG CACTAGGCCG AGACAGTGTG GGTGCTGCCT CAGTCAACCT AACGCCGTCC 
GACCTGCCCC AAGATGTGTA CAGGGAGGTG GCAACACAGG TGGAGGAGTT CCGCCAGCAG 
GACGCCAAGG AGGGTCTACG GGTGGCTCAG GTGCTGGAGG GCTTCATCAG CCGCAAGGTG 
GTGAAGCAGA CAGTGATGAC GGTGGTGTAT GGGGTCACAC GCTACGGAGG GCGCCTGCAG 
ATAGAGAAGC GCCTGCGCGA ACTCAGCGAC TTCCCTCAGG AGTTTGTCTG GGAAGCCTCA 
CACTACCTCG TGCGCCAGGT CTTCAAAAGT CTACAGGAGA TGTTCACCAG CACGCGGGCC 
ATTCAGCACT GGCTGACTGA GAGTGCCAAC CTCATCTCTC ACGCCGGATG GCCTGTGGAG 
TGGGTCACGC CCCTGGGCAT CCCCATCATA CAGCCCTATC ACCGCGAGTC CAAGGTCCAG 
GTACTTGGGA AATGCCCTTC TCCTTTTCCC TCATCTCCCC TCCTCCCTGC CAAGGGCTGG 
ACTCACCCCT CCTCCAACCC CGGTCTGCAG GTAAAAGGTG GCCTCCAGAG CATCACCCTC 
ACCAGCTCGG TAGATGAGAG TCAGAAGCCC AACACTCTGA AGCAGAAAAA TGGCTTCCCG 
CCCAACTTCA TCCACTCCCT GGACTCCTCC CACATGATGC TGACCGCCCT ACACTGCTAC 
AGGAAGGGCC TGATTTTCGT CTCCGTGCAC GACTGCTTCT GGACACATGC CGCTGACATC 
CCCACGATGA ACGAGGTATG CCGCGAGCAA TTCGTGCGCC TGCACAGCCA GCCCATCCTG 
GAAGACCTGG CCAAGTTCCT GAAGAAGCGT TTCTGCTCCG TGTCCAGCAT CAAGTCCCTA 
AAGTCCTCGG AGCGCGCCCT GGTCACCAAG CTGCAGGAGA CACTACAGTC CTTGCCAAAG 
ACAGGTACCT TCGATCTAGG ACAGGTGATA AGATCCACCT ACTTTTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polrmt ( NM_172551.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766139.2 Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), mRNA.

Price & Availability↑

CloneID	OMu13377
Clone ID Related Accession (Same CDS sequence)	NM_172551.3
Accession Version	NM_172551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK030039.1, AK053356.1 and AK042071.1. On Dec 17, 2009 this sequence version replaced NM_172551.2. ##Evidence-Data-START## Transcript exon combination :: AK154729.1, BC117940.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCGGCGC TCCGGTGGAC CCGAAGCGCA GCCGGGCTTG GCCGGGTTCT GCGCTCCCCT 
GGGCCCCACC GCCCGCCTTC GGAGGAAGGG ACTTTTGGTG GCTTCTGCAG CTCAAGAAGG 
AGCTCCGCTG CCAGTCCCCG TGAGCAACAT GTCCTGAGGG AGTGGGGCCA TGCTGAACTG 
CTGGAAGTGC TGGAGGCTCG GGTGCGGCAG CTCCGGGCAG AGGGCACACC TGAGATGAGG 
GTGAAGAAGG TGCAGGTGGA CCGGCCTCCA CAGGGCCACA GCAGCCGCTG GGCGCAAAAG 
CTAGAGGCTG AGAAAAGGGT GAAGCAGAGG CGCCAAAAGG AAGTTGACCA GCAGAAGCAA 
GCCCTCACAC AGGAGTTCTG GACCCTTCAC AAGGAGCCCA AGATCTGGAA CAAGAAGCTG 
GCTGGCTACC TGCAGCCAAG CAAGAAGGGA ACACCCACGA ACTCAGAGGA AAAGCAGCTG 
GCCCAGGCCC TTCAGGCTGC TCTGGGGAGG CTCAGCTCCC GTGAGGCAGA GGCCCTGGCC 
AGGAAGAAAG CCAAGGCGGT GGAGGCGCAG ATCCTGGTCC TCCAGCAGAA GTTCCTGGCT 
TTCTTTGAGT GCTGCGTCTG CACTGGCCAA GTGCCCCTCG CTCACCACGT GCTGGTCACT 
CACCATAACA ACGGAGACAG ACAGCAGGTG CTCACACTGC ACATGTACAA CACCGTGATG 
CTTGGCTGGG CCCGCAAGGG CTCCTTCAGA GAGCTGGTCT ATGTGTTCCT CATGCTGAAG 
GATGCTGGCC TCTCCCCAGA CCTGTGCTCC TATGCAGCTG CACTCCAGTG CATGGGACGC 
AGGGACCAGG ATGTTCGCAC CATCCAGAGG TGTCTGAAGC AGATGATGGA AGAAGGCTTC 
CAGCCCCAGC TGCTCTTCAC TGACCTGGTC CTGGAAGAGG AGGACCGGGC CGCGCTCCTG 
AGAGCAGTGG TCAAGGCTGA GCCTGCCTTT CGTCCACCGC CACAGGCCCC AAGCCCAGTT 
AACACATCCA CGCTGCTCAA GGACATATAT AGCAAGGAGG GCCCTGTGTC CTACCCAAAG 
CTGCACCTGC CCCTGGACAC CCTGCAGGAC CTCTTCTACC AGCAGCTGCA TGTGGAGCTG 
AGCTCCAGTG TCTGTGTCCA GTCGGTGGAG AAGGCTCCAG TAATGTCCAA GGAGGTCATT 
GAGGCGCGGA AGACCCTGCA GGCCCTGCGG GAGCAGTGGG AAGTGGAGCT GTTGCGCGTG 
CTGCGGGAGA CAAAGGCCAC TATGGGCCGC CAGGCCTATG AAGGCCAGCC CACCCTCTAC 
CCGTTCCTGT GCCTGCTGAG CGAAGGGGAG TTTGTGAGCA TACTGATGCA GGTTCTGAAG 
GTCCTGCCTG CGCAGGGTGA GCCCCTTATC CAGTTGGCCC ATAACCTGGG CCTTCGGGTG 
TTAAACCGGC ATTTGGTGAA GCAGAAGCAG GTCACTAACC ATGTGCAGAA GCTGGGGCAG 
CGGTACTCGC AGTACCTCCA GCTGCTGGCG TCTGACACCC AGGTGGCGCC CTGCCTTCCT 
CGGGAGTACT GGGAATCGCT GGGCCCACTG GAGGCCCCTG CACAACAGCC CTGGTCCGTG 
CCAGTGCTGC TACAGCTGGG CAAGCAATTG GCGGAGCTGC TGGTGCAGGC AGTGCAGATG 
CCGCGCAGCC TGGCGGCCCG GCAGGGTGCT CAGCGCTCCA TCCCAGTGCT TTACCATGTG 
TATTCCTTCC GAAGCTACCG CCAGGTGGGC ATCCTTAAGC CTCACCCTGC CTTCACGCAC 
CTGCTAGAGA CGGCAGCAGA GCCCACACTG ACCTTCGAGA CCACGGAAGT GCCCATGCTG 
TGCCCACCAC TGCCCTGGAC GTCGCTGCAT TCTGGTGCCT ACTTGCTGAG CTCCACCAAA 
CTAATGCGTG CCACGGAGGG TACCACACAG CACCAGCGTC TGCTGGAGCA ATGCCCTCCT 
GCCCAGCTGC ACGGCCCCCT GGATGCGCTC ACACAGTTGG GGAACTGCGC CTGGCGTGTA 
AACGGGCATC TGCTGGACTT GGTGCTGCAG ATCTTTAGAG ACAAGGGCTG TATGCCCTTG 
GGCGTGCCTC CCCCGCGCTC AGAAGCACCG CGGCCGGCCC GGTATCAGCT GCCACCCGGC 
TCCACACCAG TGCACAAGTC TGAGCTGCGG AAGGAGCTGG CGCGTTGCCT CAAGGTGGCT 
CGAGAGATGC ACAGTCTGCG CAGCGAGGCC CTGTATCGCC TGTCGCTGGC ACAGCACCTA 
CGCCACCGTG TCTTCTGGTT GCCGCACAAC ATGGACTTCC GCGGCCGCAC TTACCCCTGC 
CCGCCACACT TCAACCACCT GGGCAGTGAC CTAGCGCGTG CGCTATTGGA GTTTGCTGAG 
GGCCGGCCAC TGGGACCACG TGGTCTGGAC TGGCTTAAGA TCCACCTGAT CAACCTGACT 
GGCCTCAAGA AGGGAGACTC GCTGCGCATG CGCCTAGCCT TCGCAGATGA GGTCATGGAG 
GAGATCTTGG ACTCTGCAGA CAACCCCTTG ACGGGCCGGA AGTGGTGGAT GGAAGCTGAT 
GAGCCCTGGC AGACCCTGGC CTGCTGCATG GAGGTGGCAC ACGCAGTCCG GTCCCCAGAC 
CCTGCTGCCT ACATTTCCCA CCTGCCAGTT CACCAGGATG GCTCCTGCAA TGGCTTACAG 
CATTACGCCG CACTAGGCCG AGACAGTGTG GGTGCTGCCT CAGTCAACCT AACGCCGTCC 
GACCTGCCCC AAGATGTGTA CAGGGAGGTG GCAACACAGG TGGAGGAGTT CCGCCAGCAG 
GACGCCAAGG AGGGTCTACG GGTGGCTCAG GTGCTGGAGG GCTTCATCAG CCGCAAGGTG 
GTGAAGCAGA CAGTGATGAC GGTGGTGTAT GGGGTCACAC GCTACGGAGG GCGCCTGCAG 
ATAGAGAAGC GCCTGCGCGA ACTCAGCGAC TTCCCTCAGG AGTTTGTCTG GGAAGCCTCA 
CACTACCTCG TGCGCCAGGT CTTCAAAAGT CTACAGGAGA TGTTCACCAG CACGCGGGCC 
ATTCAGCACT GGCTGACTGA GAGTGCCAAC CTCATCTCTC ACGCCGGATG GCCTGTGGAG 
TGGGTCACGC CCCTGGGCAT CCCCATCATA CAGCCCTATC ACCGCGAGTC CAAGGTCCAG 
GTAAAAGGTG GCCTCCAGAG CATCACCCTC ACCAGCTCGG TAGATGAGAG TCAGAAGCCC 
AACACTCTGA AGCAGAAAAA TGGCTTCCCG CCCAACTTCA TCCACTCCCT GGACTCCTCC 
CACATGATGC TGACCGCCCT ACACTGCTAC AGGAAGGGCC TGATTTTCGT CTCCGTGCAC 
GACTGCTTCT GGACACATGC CGCTGACATC CCCACGATGA ACGAGGTATG CCGCGAGCAA 
TTCGTGCGCC TGCACAGCCA GCCCATCCTG GAAGACCTGG CCAAGTTCCT GAAGAAGCGT 
TTCTGCTCCG TGTCCAGCAT CAAGTCCCTA AAGTCCTCGG AGCGCGCCCT GGTCACCAAG 
CTGCAGGAGA CACTACAGTC CTTGCCAAAG ACAGGTACCT TCGATCTAGG ACAGGTGATA 
AGATCCACCT ACTTTTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766139.2
CDS	30..3653
Translation	MSALRWTRSAAGLGRVLRSPGPHRPPSEEGTFGGFCSSRRSSAASPREQHVLREWGHAELLEVLEARVRQLRAEGTPEMRVKKVQVDRPPQGHSSRWAQKLEAEKRVKQRRQKEVDQQKQALTQEFWTLHKEPKIWNKKLAGYLQPSKKGTPTNSEEKQLAQALQAALGRLSSREAEALARKKAKAVEAQILVLQQKFLAFFECCVCTGQVPLAHHVLVTHHNNGDRQQVLTLHMYNTVMLGWARKGSFRELVYVFLMLKDAGLSPDLCSYAAALQCMGRRDQDVRTIQRCLKQMMEEGFQPQLLFTDLVLEEEDRAALLRAVVKAEPAFRPPPQAPSPVNTSTLLKDIYSKEGPVSYPKLHLPLDTLQDLFYQQLHVELSSSVCVQSVEKAPVMSKEVIEARKTLQALREQWEVELLRVLRETKATMGRQAYEGQPTLYPFLCLLSEGEFVSILMQVLKVLPAQGEPLIQLAHNLGLRVLNRHLVKQKQVTNHVQKLGQRYSQYLQLLASDTQVAPCLPREYWESLGPLEAPAQQPWSVPVLLQLGKQLAELLVQAVQMPRSLAARQGAQRSIPVLYHVYSFRSYRQVGILKPHPAFTHLLETAAEPTLTFETTEVPMLCPPLPWTSLHSGAYLLSSTKLMRATEGTTQHQRLLEQCPPAQLHGPLDALTQLGNCAWRVNGHLLDLVLQIFRDKGCMPLGVPPPRSEAPRPARYQLPPGSTPVHKSELRKELARCLKVAREMHSLRSEALYRLSLAQHLRHRVFWLPHNMDFRGRTYPCPPHFNHLGSDLARALLEFAEGRPLGPRGLDWLKIHLINLTGLKKGDSLRMRLAFADEVMEEILDSADNPLTGRKWWMEADEPWQTLACCMEVAHAVRSPDPAAYISHLPVHQDGSCNGLQHYAALGRDSVGAASVNLTPSDLPQDVYREVATQVEEFRQQDAKEGLRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFTSTRAIQHWLTESANLISHAGWPVEWVTPLGIPIIQPYHRESKVQVKGGLQSITLTSSVDESQKPNTLKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLIFVSVHDCFWTHAADIPTMNEVCREQFVRLHSQPILEDLAKFLKKRFCSVSSIKSLKSSERALVTKLQETLQSLPKTGTFDLGQVIRSTYFFS

Target ORF information:

RefSeq Version	NM_172551.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) mitochondrial (DNA directed) (Polrmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172551.3

ORF Insert Sequence:

ATGTCGGCGC TCCGGTGGAC CCGAAGCGCA GCCGGGCTTG GCCGGGTTCT GCGCTCCCCT 
GGGCCCCACC GCCCGCCTTC GGAGGAAGGG ACTTTTGGTG GCTTCTGCAG CTCAAGAAGG 
AGCTCCGCTG CCAGTCCCCG TGAGCAACAT GTCCTGAGGG AGTGGGGCCA TGCTGAACTG 
CTGGAAGTGC TGGAGGCTCG GGTGCGGCAG CTCCGGGCAG AGGGCACACC TGAGATGAGG 
GTGAAGAAGG TGCAGGTGGA CCGGCCTCCA CAGGGCCACA GCAGCCGCTG GGCGCAAAAG 
CTAGAGGCTG AGAAAAGGGT GAAGCAGAGG CGCCAAAAGG AAGTTGACCA GCAGAAGCAA 
GCCCTCACAC AGGAGTTCTG GACCCTTCAC AAGGAGCCCA AGATCTGGAA CAAGAAGCTG 
GCTGGCTACC TGCAGCCAAG CAAGAAGGGA ACACCCACGA ACTCAGAGGA AAAGCAGCTG 
GCCCAGGCCC TTCAGGCTGC TCTGGGGAGG CTCAGCTCCC GTGAGGCAGA GGCCCTGGCC 
AGGAAGAAAG CCAAGGCGGT GGAGGCGCAG ATCCTGGTCC TCCAGCAGAA GTTCCTGGCT 
TTCTTTGAGT GCTGCGTCTG CACTGGCCAA GTGCCCCTCG CTCACCACGT GCTGGTCACT 
CACCATAACA ACGGAGACAG ACAGCAGGTG CTCACACTGC ACATGTACAA CACCGTGATG 
CTTGGCTGGG CCCGCAAGGG CTCCTTCAGA GAGCTGGTCT ATGTGTTCCT CATGCTGAAG 
GATGCTGGCC TCTCCCCAGA CCTGTGCTCC TATGCAGCTG CACTCCAGTG CATGGGACGC 
AGGGACCAGG ATGTTCGCAC CATCCAGAGG TGTCTGAAGC AGATGATGGA AGAAGGCTTC 
CAGCCCCAGC TGCTCTTCAC TGACCTGGTC CTGGAAGAGG AGGACCGGGC CGCGCTCCTG 
AGAGCAGTGG TCAAGGCTGA GCCTGCCTTT CGTCCACCGC CACAGGCCCC AAGCCCAGTT 
AACACATCCA CGCTGCTCAA GGACATATAT AGCAAGGAGG GCCCTGTGTC CTACCCAAAG 
CTGCACCTGC CCCTGGACAC CCTGCAGGAC CTCTTCTACC AGCAGCTGCA TGTGGAGCTG 
AGCTCCAGTG TCTGTGTCCA GTCGGTGGAG AAGGCTCCAG TAATGTCCAA GGAGGTCATT 
GAGGCGCGGA AGACCCTGCA GGCCCTGCGG GAGCAGTGGG AAGTGGAGCT GTTGCGCGTG 
CTGCGGGAGA CAAAGGCCAC TATGGGCCGC CAGGCCTATG AAGGCCAGCC CACCCTCTAC 
CCGTTCCTGT GCCTGCTGAG CGAAGGGGAG TTTGTGAGCA TACTGATGCA GGTTCTGAAG 
GTCCTGCCTG CGCAGGGTGA GCCCCTTATC CAGTTGGCCC ATAACCTGGG CCTTCGGGTG 
TTAAACCGGC ATTTGGTGAA GCAGAAGCAG GTCACTAACC ATGTGCAGAA GCTGGGGCAG 
CGGTACTCGC AGTACCTCCA GCTGCTGGCG TCTGACACCC AGGTGGCGCC CTGCCTTCCT 
CGGGAGTACT GGGAATCGCT GGGCCCACTG GAGGCCCCTG CACAACAGCC CTGGTCCGTG 
CCAGTGCTGC TACAGCTGGG CAAGCAATTG GCGGAGCTGC TGGTGCAGGC AGTGCAGATG 
CCGCGCAGCC TGGCGGCCCG GCAGGGTGCT CAGCGCTCCA TCCCAGTGCT TTACCATGTG 
TATTCCTTCC GAAGCTACCG CCAGGTGGGC ATCCTTAAGC CTCACCCTGC CTTCACGCAC 
CTGCTAGAGA CGGCAGCAGA GCCCACACTG ACCTTCGAGA CCACGGAAGT GCCCATGCTG 
TGCCCACCAC TGCCCTGGAC GTCGCTGCAT TCTGGTGCCT ACTTGCTGAG CTCCACCAAA 
CTAATGCGTG CCACGGAGGG TACCACACAG CACCAGCGTC TGCTGGAGCA ATGCCCTCCT 
GCCCAGCTGC ACGGCCCCCT GGATGCGCTC ACACAGTTGG GGAACTGCGC CTGGCGTGTA 
AACGGGCATC TGCTGGACTT GGTGCTGCAG ATCTTTAGAG ACAAGGGCTG TATGCCCTTG 
GGCGTGCCTC CCCCGCGCTC AGAAGCACCG CGGCCGGCCC GGTATCAGCT GCCACCCGGC 
TCCACACCAG TGCACAAGTC TGAGCTGCGG AAGGAGCTGG CGCGTTGCCT CAAGGTGGCT 
CGAGAGATGC ACAGTCTGCG CAGCGAGGCC CTGTATCGCC TGTCGCTGGC ACAGCACCTA 
CGCCACCGTG TCTTCTGGTT GCCGCACAAC ATGGACTTCC GCGGCCGCAC TTACCCCTGC 
CCGCCACACT TCAACCACCT GGGCAGTGAC CTAGCGCGTG CGCTATTGGA GTTTGCTGAG 
GGCCGGCCAC TGGGACCACG TGGTCTGGAC TGGCTTAAGA TCCACCTGAT CAACCTGACT 
GGCCTCAAGA AGGGAGACTC GCTGCGCATG CGCCTAGCCT TCGCAGATGA GGTCATGGAG 
GAGATCTTGG ACTCTGCAGA CAACCCCTTG ACGGGCCGGA AGTGGTGGAT GGAAGCTGAT 
GAGCCCTGGC AGACCCTGGC CTGCTGCATG GAGGTGGCAC ACGCAGTCCG GTCCCCAGAC 
CCTGCTGCCT ACATTTCCCA CCTGCCAGTT CACCAGGATG GCTCCTGCAA TGGCTTACAG 
CATTACGCCG CACTAGGCCG AGACAGTGTG GGTGCTGCCT CAGTCAACCT AACGCCGTCC 
GACCTGCCCC AAGATGTGTA CAGGGAGGTG GCAACACAGG TGGAGGAGTT CCGCCAGCAG 
GACGCCAAGG AGGGTCTACG GGTGGCTCAG GTGCTGGAGG GCTTCATCAG CCGCAAGGTG 
GTGAAGCAGA CAGTGATGAC GGTGGTGTAT GGGGTCACAC GCTACGGAGG GCGCCTGCAG 
ATAGAGAAGC GCCTGCGCGA ACTCAGCGAC TTCCCTCAGG AGTTTGTCTG GGAAGCCTCA 
CACTACCTCG TGCGCCAGGT CTTCAAAAGT CTACAGGAGA TGTTCACCAG CACGCGGGCC 
ATTCAGCACT GGCTGACTGA GAGTGCCAAC CTCATCTCTC ACGCCGGATG GCCTGTGGAG 
TGGGTCACGC CCCTGGGCAT CCCCATCATA CAGCCCTATC ACCGCGAGTC CAAGGTCCAG 
GTAAAAGGTG GCCTCCAGAG CATCACCCTC ACCAGCTCGG TAGATGAGAG TCAGAAGCCC 
AACACTCTGA AGCAGAAAAA TGGCTTCCCG CCCAACTTCA TCCACTCCCT GGACTCCTCC 
CACATGATGC TGACCGCCCT ACACTGCTAC AGGAAGGGCC TGATTTTCGT CTCCGTGCAC 
GACTGCTTCT GGACACATGC CGCTGACATC CCCACGATGA ACGAGGTATG CCGCGAGCAA 
TTCGTGCGCC TGCACAGCCA GCCCATCCTG GAAGACCTGG CCAAGTTCCT GAAGAAGCGT 
TTCTGCTCCG TGTCCAGCAT CAAGTCCCTA AAGTCCTCGG AGCGCGCCCT GGTCACCAAG 
CTGCAGGAGA CACTACAGTC CTTGCCAAAG ACAGGTACCT TCGATCTAGG ACAGGTGATA 
AGATCCACCT ACTTTTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TEFM regulates both transcription elongation and RNA processing in mitochondria.
EMBO reports20(6)(2019 06)
Jiang S,Koolmeister C,Misic J,Siira S,Kühl I,Silva Ramos E,Miranda M,Jiang M,Posse V,Lytovchenko O,Atanassov I,Schober FA,Wibom R,Hultenby K,Milenkovic D,Gustafsson CM,Filipovska A,Larsson NG

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.
eLife6(2017 11)
Kühl I,Miranda M,Atanassov I,Kuznetsova I,Hinze Y,Mourier A,Filipovska A,Larsson NG

POLRMT regulates the switch between replication primer formation and gene expression of mammalian mtDNA.
Science advances2(8)e1600963(2016 08)
Kühl I,Miranda M,Posse V,Milenkovic D,Mourier A,Siira SJ,Bonekamp NA,Neumann U,Filipovska A,Polosa PL,Gustafsson CM,Larsson NG

POLRMT does not transcribe nuclear genes.
Nature514(7521)E7-11(2014 Oct)
Kühl I,Kukat C,Ruzzenente B,Milenkovic D,Mourier A,Miranda M,Koolmeister C,Falkenberg M,Larsson NG

A novel AMPK-dependent FoxO3A-SIRT3 intramitochondrial complex sensing glucose levels.
Cellular and molecular life sciences : CMLS70(11)2015-29(2013 Jun)
Peserico A,Chiacchiera F,Grossi V,Matrone A,Latorre D,Simonatto M,Fusella A,Ryall JG,Finley LW,Haigis MC,Villani G,Puri PL,Sartorelli V,Simone C

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Polrmt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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