mtRNApol cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtRNApol
Entrez Gene ID	33285
Full Name	mitochondrial RNA polymerase
Synonyms	CG4644,Dmel\CG4644,POLRMT,mt RNA pol,mtRNA-pol,mtRNAP
General protein information	Preferred Names mitochondrial RNA polymerase Names CG4644 gene product from transcript CG4644-RB CG4644-PA CG4644-PB mtRNApol-PA mtRNApol-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21F1-21F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272924.1	NP_001259853.1	mitochondrial RNA polymerase, isoform B
NM_134721.3	NP_608565.1	mitochondrial RNA polymerase, isoform A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	polrmt	XP_004910992.1
Oryza sativa (rice)	Os06g0652000	NP_001058231.2
Canis lupus familiaris (dog)	POLRMT	XP_005633227.1
Gallus gallus (chicken)	POLRMT	XP_418204.3
Arabidopsis thaliana (thale cress)	SCA3	NP_179989.1
Mus musculus (house mouse)	Polrmt	NP_766139.2
Pan troglodytes (chimpanzee)	POLRMT	XP_003316023.1
Bos taurus (cattle)	POLRMT	NP_001192480.1
Danio rerio (zebrafish)	si:dkey-21e2.1	XP_005170578.1
Homo sapiens (human)	POLRMT	NP_005026.3
Rattus norvegicus (Norway rat)	Polrmt	NP_001100236.1
Drosophila melanogaster (fruit fly)	mtRNApol	NP_001259853.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mtRNApol gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtRNApol cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13425	NM_001272924.1 Latest version!	Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFa13425	NM_134721.3 Latest version!	Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtRNApol ( NM_001272924.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259853.1 Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13425
Clone ID Related Accession (Same CDS sequence)	NM_001272924.1 , NM_134721.3
Accession Version	NM_001272924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial RNA polymerase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCGCT TGCTGAGTGC AAATCGGGCG GCGGCCGTGC AAAAAGCACT GCGAGTGCCA 
CCCACTGCTC ACCCAACAAC AACAACAGCT ACAACAACAA CAGTGTGTTG CGGCTGCGGA 
ATTGCGCCTT CAAATTTCGT GCATTATCAG CAGTATTATC AAATGCTCTA CCGCCAGATG 
CCGGCAGGAG CAATTACAGC AAAAACAACA ACGACAACCA CTCGCACACA AACAACAACA 
CCCACACAAA CGGATGCAGT GGCCCAGGCG AAAAAGAAGC GATTGCGCAA GCCGCGCTAC 
GCCAAATACG TGGAGCTCCT CGAGGTCACC GAACAAGCCA TCAGCGAGCG AAGGTCGCGT 
GTGAAGCGGC TGAAATCCAA GAAGCTGGCC GACTTCATAC ATAAGGCCCA GCAGCAAATT 
CTGGAGCGAC GCGCCCGCGA AGATCGCAAG GATAAGAAAA CCAAATGTGT ACTGGATCTG 
GCGCAGCGTC AGCCTGTAGA ATATCAGAAA ATCGATATGG AGGCTTTGGA TGGATCTATT 
CTAGAGTCTG GAGTATACGA CGATCGGCCT ATGCTCTTCT TTGGCAAGGA TGAGTACACA 
AAACAGGTGA TAGCGAATCC CGAGGAGGTG CAAAACATTC TCAAAAGCTT CCAAAACTTT 
CGGGACATCA TCGAGAGCAT GGAAAATGAT CAGCATGAAG CGGATTTTGG TCTGAGCACC 
ACAAAAGTGC ATCCTGAAGA ACACGAAATG CTGGATCCAT TCGAGGCCAT TGAGCCGTAC 
ATCAGTCCCC TGGACAAGAG CAAACGTTCA GTAACTACTG TGGCCGTGCC CAACGCCAGC 
GCAGTCACAA CCAAGTCGAA GAAACGTTTC AAGTCAAAGG TTCACGTAAA CGAGGAGCAA 
GAGCGTCTGG CCAAGCAACG GGCACTGCAC ATAAACCTGA ACACATATCT GAATGTGTGT 
GTCTCGGCCA ACATGCTAAA TCGCGCCCTG TCCACCATCA TTGCCTATCG CGGCAAGGTG 
AGGAAGAGTT CTTTGCCGCA CATGTCCGAA GGCCTCATCA CCATCGACCT GTACAACATC 
CTTCTGCACG GCTATGCGGG CAAGGGATCC TTCGATCGAG CCCAGGAGGT CTTCAAGCTC 
ATCGAAGAGG ACGGACTGTC GTTCAACGAG CAAACGTACG CAGCCATTTT CGAGTGCTTG 
GGTCGCCTGG AACCGGATGA GGAGAACCAC CAATACATAT CCGAATACGT TTCCAAAGCC 
GAAAAGGAAG GATTCAGTTT GAATCAAATT ATGGACAGGT CAAAGTTTGT GGCTGACCAG 
CGGGACATTG CTTTGGATGC GATAAGACGG CTTCGACCGG ACTTTAAGCC GGTCTATGTG 
CCACCGCAAC TCGGATACGA CAACGAACTG CTGGATCACC TCAACGAGCA TGTTATACCT 
GTTGGAGCTA AGAAAGAACC GGGAAAAGCT GACTACGCCA TAATGAACTC CAAGCGAGGC 
TATACCGCAG CTCAATTGGA ACAGCTAGCT CGGGAGCAAC TTAAAATTGA GTTGGACGGC 
AGCATTACCA TCAAGTCCAT CGAGAAATCC AAGGAGTTCG CCAACTCCGA AAAATGCCGG 
GAACTTCTAA AAGAACTGGA GGACACGTGG CGCAAACAAA TTTCGGCAGC CATTGTGCGC 
GATCTCAACA CGCTGCGTGC ACAAGTGCGC TTCAAGCCAC ACGGCTTCAT GAACTACTAC 
ACGTATCTAA AGACCCTTGA CGCCTCGCAC TTTGCGGACA TCCTGATTAA GGAGCTGTAC 
AAGCTGGCTG AGGGCTCTGA GACATTTAGT CCAACAGTGG GTCAGCTGTA CAAAGAACTC 
GGGCAGAAGG TGCAGCAACG ATACCAAATT GAGCAGAAGA AACACAACGG CACGCTGGAG 
AAGATCGGCG AGATATACAG TTCGTATTGT GAATTGTGGG GCAGCGGGAT GTCGCAGGAT 
AACACGCGTC AGGCTTGGCA GCGTCTCGTG TACGAGCAGC GGGAGAGTGG ACCAAGCATG 
GATCTGCTGG AGGTGCCGTG GCCGTCTAAT GTCCTAACAG GCGTGGGTCG ATTTTTGTAC 
AACATTCTGA TGAGGGACAT CAAGATCGAC GCCCACATAA TGAGGCAGAA GAGCAAAAGC 
AAAGCGGCCG CTCAGCCACA AAATCTGTTG CCCGCCTTTT ACACTCTTTT CCGGAATCAA 
GGACGCTTTG TTAAAGAGGA AGTCAAGCCG CATCCGGTGC TCTCGAGATT ATTCCGAGCG 
TCCCGCCAAC AGACGCTCAC GTTCGACTCC AACCTAGTGC CCATGCTGTG TCCCCCGCAA 
CCGTGGAGTA CTCCGCACAA TGGTGGCTAC CTGCTGAACA AGACTGAGCT CATTCGCCTG 
CCTCATCAAG CCATCCAGCA GTGGGACCGC ATTATGGCTT CCAACCCGCA GCACCTTTAT 
CCTGCCCTAG ATTCTCTGAA TCAGTTGGCC AGTGTGCCTT GGCGGGTGAA CACTCAGTTG 
CTGGATGTGA TTATAAAGGT GTTCCAGAAC GGAGGCGATG TGAAATTGGA CGTCCCTCAG 
CCACCCAGTT CCCTGCCGCC GCTTCCCACG TTGCCAGCTA AGGACGCCGA AGGCAATGCC 
ACCTCCAGTG AGGACCGAGC TAAGCAGTTC CGGGACAAGC TCGGACATCG AAGGAAGCAG 
GCCGAGATGT ACAGCTTGTG GTGCGATGCT CTCTATAGGT TGTCACTGGC ACAGCATTAC 
CGTGATAAGG TCTTCTGGCT GCCACACAAC ATGGACTTCC GTGGACGTGT CTATCCTGTG 
CCGCCGCATC TCAATCACCT GGGCTCGGAC CTGGCTCGTT CGATGCTCAT CTTCGATCAG 
GCGCAGCCAC TGGGAGTCGA TGGATTAAGT TGGCTTAAGC TGCACTGCAT CAATCTGACT 
GGTCTCAAGA AGCGGGACTC GGTGAGGGAA CGTCTTTTGT ACGCGGAAGA GATCATGCCA 
GATATCCTGG ACTCTGCGGA CAATCCACTC ACCGGGCGGA TGTGGTGGTC CAAATCGGAT 
GAGCCATGGC AAACGCTGGC GTGCTGCATG GAAATAGCCA ATGTTCATCG CTGTCCCGAT 
CCCGCTGCCT ATCTAAGTCG CTTCCCCATA CACCAAGACG GTTCCTGCAA TGGATTGCAG 
CACTACGCTG CCTTGGGCAG AGATGAAGCT GGTGCTCGTA GTGTCAACCT GGCACCATCG 
GCCATTCCGC AGGACGTTTA CAGTGCGGTA GCCGCTTTGG TGGAGAAGAG CAGGAAAGCG 
GATGCCCAGA ACGGTCTCCA TGTGGCGGAA GCACTGGCCG GATTCGTTCG CCGCAAGGTG 
ATCAAACAAA CAGTGATGAC AACGGTCTAC GGAGTGACTC GTTACGGAGC CAGACTGCAG 
ATAGCGCGCC AGCTAAAAGA CATTGACGAC TTCCCCAAGG ACTGGGTCTG GCCTGCGTCC 
ACATACTTGA CCACCAAGAC GTTCGAAAGC CTGCGGGAAA TGTTCACATC CACGAGAGAA 
ATTCAGGATT GGTTCACGGA GTGCGCCCGC TTGATTTCCG GCGTGTGCAG CCAGAACGTG 
GAGTGGGTCA CGCCACTCGG CCTGCCGGTG GTGCAGCCCT ACAACCGCCA GGAGATGAAG 
CACTCGCCTC GCAGCGGCTT CAAGGTCTCC GCCAACATGC CCATGGACTT GTACGAGCGG 
CCAAACATCC TCAAGCAGAA GAACGCCTTC CCGCCGAACT TCATCCACTC GCTGGACTCC 
TCGCACATGA TGCTCACCTC GCTGCACTGC GAGCGGCAGG GCATCACGTT TGTTTCGGTG 
CACGACTGCT TCTGGACGCA CGCCAACACC GTGCCCGAGC TCAACCGGAT GTGCCGGGAG 
CAGTTTGTGG CCCTGCACTC GCAGCCCATT TTGGAGCAGC TCTCCGAGTT CATGCGACAT 
ACCTACAGCT TTAAGGACAG TGACTTCATC AACGATGGTT CCGTGGAGGA TCTGTCCAAG 
CGCCAGCTGA ACCGCATTCT GAAGCAGATG CCCCAGAAGG GAGACTTTGA CCTGCGCAAC 
GTGCTCGACT CAGTGTACTT CTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259853.1
CDS	172..4281
Translation	MYRLLSANRAAAVQKALRVPPTAHPTTTTATTTTVCCGCGIAPSNFVHYQQYYQMLYRQMPAGAITAKTTTTTTRTQTTTPTQTDAVAQAKKKRLRKPRYAKYVELLEVTEQAISERRSRVKRLKSKKLADFIHKAQQQILERRAREDRKDKKTKCVLDLAQRQPVEYQKIDMEALDGSILESGVYDDRPMLFFGKDEYTKQVIANPEEVQNILKSFQNFRDIIESMENDQHEADFGLSTTKVHPEEHEMLDPFEAIEPYISPLDKSKRSVTTVAVPNASAVTTKSKKRFKSKVHVNEEQERLAKQRALHINLNTYLNVCVSANMLNRALSTIIAYRGKVRKSSLPHMSEGLITIDLYNILLHGYAGKGSFDRAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEENHQYISEYVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPVYVPPQLGYDNELLDHLNEHVIPVGAKKEPGKADYAIMNSKRGYTAAQLEQLAREQLKIELDGSITIKSIEKSKEFANSEKCRELLKELEDTWRKQISAAIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKLAEGSETFSPTVGQLYKELGQKVQQRYQIEQKKHNGTLEKIGEIYSSYCELWGSGMSQDNTRQAWQRLVYEQRESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYTLFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGGYLLNKTELIRLPHQAIQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVPQPPSSLPPLPTLPAKDAEGNATSSEDRAKQFRDKLGHRRKQAEMYSLWCDALYRLSLAQHYRDKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRDSVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRCPDPAAYLSRFPIHQDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTECARLISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS

Target ORF information:

RefSeq Version	NM_001272924.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272924.1

ORF Insert Sequence:

ATGTATCGCT TGCTGAGTGC AAATCGGGCG GCGGCCGTGC AAAAAGCACT GCGAGTGCCA 
CCCACTGCTC ACCCAACAAC AACAACAGCT ACAACAACAA CAGTGTGTTG CGGCTGCGGA 
ATTGCGCCTT CAAATTTCGT GCATTATCAG CAGTATTATC AAATGCTCTA CCGCCAGATG 
CCGGCAGGAG CAATTACAGC AAAAACAACA ACGACAACCA CTCGCACACA AACAACAACA 
CCCACACAAA CGGATGCAGT GGCCCAGGCG AAAAAGAAGC GATTGCGCAA GCCGCGCTAC 
GCCAAATACG TGGAGCTCCT CGAGGTCACC GAACAAGCCA TCAGCGAGCG AAGGTCGCGT 
GTGAAGCGGC TGAAATCCAA GAAGCTGGCC GACTTCATAC ATAAGGCCCA GCAGCAAATT 
CTGGAGCGAC GCGCCCGCGA AGATCGCAAG GATAAGAAAA CCAAATGTGT ACTGGATCTG 
GCGCAGCGTC AGCCTGTAGA ATATCAGAAA ATCGATATGG AGGCTTTGGA TGGATCTATT 
CTAGAGTCTG GAGTATACGA CGATCGGCCT ATGCTCTTCT TTGGCAAGGA TGAGTACACA 
AAACAGGTGA TAGCGAATCC CGAGGAGGTG CAAAACATTC TCAAAAGCTT CCAAAACTTT 
CGGGACATCA TCGAGAGCAT GGAAAATGAT CAGCATGAAG CGGATTTTGG TCTGAGCACC 
ACAAAAGTGC ATCCTGAAGA ACACGAAATG CTGGATCCAT TCGAGGCCAT TGAGCCGTAC 
ATCAGTCCCC TGGACAAGAG CAAACGTTCA GTAACTACTG TGGCCGTGCC CAACGCCAGC 
GCAGTCACAA CCAAGTCGAA GAAACGTTTC AAGTCAAAGG TTCACGTAAA CGAGGAGCAA 
GAGCGTCTGG CCAAGCAACG GGCACTGCAC ATAAACCTGA ACACATATCT GAATGTGTGT 
GTCTCGGCCA ACATGCTAAA TCGCGCCCTG TCCACCATCA TTGCCTATCG CGGCAAGGTG 
AGGAAGAGTT CTTTGCCGCA CATGTCCGAA GGCCTCATCA CCATCGACCT GTACAACATC 
CTTCTGCACG GCTATGCGGG CAAGGGATCC TTCGATCGAG CCCAGGAGGT CTTCAAGCTC 
ATCGAAGAGG ACGGACTGTC GTTCAACGAG CAAACGTACG CAGCCATTTT CGAGTGCTTG 
GGTCGCCTGG AACCGGATGA GGAGAACCAC CAATACATAT CCGAATACGT TTCCAAAGCC 
GAAAAGGAAG GATTCAGTTT GAATCAAATT ATGGACAGGT CAAAGTTTGT GGCTGACCAG 
CGGGACATTG CTTTGGATGC GATAAGACGG CTTCGACCGG ACTTTAAGCC GGTCTATGTG 
CCACCGCAAC TCGGATACGA CAACGAACTG CTGGATCACC TCAACGAGCA TGTTATACCT 
GTTGGAGCTA AGAAAGAACC GGGAAAAGCT GACTACGCCA TAATGAACTC CAAGCGAGGC 
TATACCGCAG CTCAATTGGA ACAGCTAGCT CGGGAGCAAC TTAAAATTGA GTTGGACGGC 
AGCATTACCA TCAAGTCCAT CGAGAAATCC AAGGAGTTCG CCAACTCCGA AAAATGCCGG 
GAACTTCTAA AAGAACTGGA GGACACGTGG CGCAAACAAA TTTCGGCAGC CATTGTGCGC 
GATCTCAACA CGCTGCGTGC ACAAGTGCGC TTCAAGCCAC ACGGCTTCAT GAACTACTAC 
ACGTATCTAA AGACCCTTGA CGCCTCGCAC TTTGCGGACA TCCTGATTAA GGAGCTGTAC 
AAGCTGGCTG AGGGCTCTGA GACATTTAGT CCAACAGTGG GTCAGCTGTA CAAAGAACTC 
GGGCAGAAGG TGCAGCAACG ATACCAAATT GAGCAGAAGA AACACAACGG CACGCTGGAG 
AAGATCGGCG AGATATACAG TTCGTATTGT GAATTGTGGG GCAGCGGGAT GTCGCAGGAT 
AACACGCGTC AGGCTTGGCA GCGTCTCGTG TACGAGCAGC GGGAGAGTGG ACCAAGCATG 
GATCTGCTGG AGGTGCCGTG GCCGTCTAAT GTCCTAACAG GCGTGGGTCG ATTTTTGTAC 
AACATTCTGA TGAGGGACAT CAAGATCGAC GCCCACATAA TGAGGCAGAA GAGCAAAAGC 
AAAGCGGCCG CTCAGCCACA AAATCTGTTG CCCGCCTTTT ACACTCTTTT CCGGAATCAA 
GGACGCTTTG TTAAAGAGGA AGTCAAGCCG CATCCGGTGC TCTCGAGATT ATTCCGAGCG 
TCCCGCCAAC AGACGCTCAC GTTCGACTCC AACCTAGTGC CCATGCTGTG TCCCCCGCAA 
CCGTGGAGTA CTCCGCACAA TGGTGGCTAC CTGCTGAACA AGACTGAGCT CATTCGCCTG 
CCTCATCAAG CCATCCAGCA GTGGGACCGC ATTATGGCTT CCAACCCGCA GCACCTTTAT 
CCTGCCCTAG ATTCTCTGAA TCAGTTGGCC AGTGTGCCTT GGCGGGTGAA CACTCAGTTG 
CTGGATGTGA TTATAAAGGT GTTCCAGAAC GGAGGCGATG TGAAATTGGA CGTCCCTCAG 
CCACCCAGTT CCCTGCCGCC GCTTCCCACG TTGCCAGCTA AGGACGCCGA AGGCAATGCC 
ACCTCCAGTG AGGACCGAGC TAAGCAGTTC CGGGACAAGC TCGGACATCG AAGGAAGCAG 
GCCGAGATGT ACAGCTTGTG GTGCGATGCT CTCTATAGGT TGTCACTGGC ACAGCATTAC 
CGTGATAAGG TCTTCTGGCT GCCACACAAC ATGGACTTCC GTGGACGTGT CTATCCTGTG 
CCGCCGCATC TCAATCACCT GGGCTCGGAC CTGGCTCGTT CGATGCTCAT CTTCGATCAG 
GCGCAGCCAC TGGGAGTCGA TGGATTAAGT TGGCTTAAGC TGCACTGCAT CAATCTGACT 
GGTCTCAAGA AGCGGGACTC GGTGAGGGAA CGTCTTTTGT ACGCGGAAGA GATCATGCCA 
GATATCCTGG ACTCTGCGGA CAATCCACTC ACCGGGCGGA TGTGGTGGTC CAAATCGGAT 
GAGCCATGGC AAACGCTGGC GTGCTGCATG GAAATAGCCA ATGTTCATCG CTGTCCCGAT 
CCCGCTGCCT ATCTAAGTCG CTTCCCCATA CACCAAGACG GTTCCTGCAA TGGATTGCAG 
CACTACGCTG CCTTGGGCAG AGATGAAGCT GGTGCTCGTA GTGTCAACCT GGCACCATCG 
GCCATTCCGC AGGACGTTTA CAGTGCGGTA GCCGCTTTGG TGGAGAAGAG CAGGAAAGCG 
GATGCCCAGA ACGGTCTCCA TGTGGCGGAA GCACTGGCCG GATTCGTTCG CCGCAAGGTG 
ATCAAACAAA CAGTGATGAC AACGGTCTAC GGAGTGACTC GTTACGGAGC CAGACTGCAG 
ATAGCGCGCC AGCTAAAAGA CATTGACGAC TTCCCCAAGG ACTGGGTCTG GCCTGCGTCC 
ACATACTTGA CCACCAAGAC GTTCGAAAGC CTGCGGGAAA TGTTCACATC CACGAGAGAA 
ATTCAGGATT GGTTCACGGA GTGCGCCCGC TTGATTTCCG GCGTGTGCAG CCAGAACGTG 
GAGTGGGTCA CGCCACTCGG CCTGCCGGTG GTGCAGCCCT ACAACCGCCA GGAGATGAAG 
CACTCGCCTC GCAGCGGCTT CAAGGTCTCC GCCAACATGC CCATGGACTT GTACGAGCGG 
CCAAACATCC TCAAGCAGAA GAACGCCTTC CCGCCGAACT TCATCCACTC GCTGGACTCC 
TCGCACATGA TGCTCACCTC GCTGCACTGC GAGCGGCAGG GCATCACGTT TGTTTCGGTG 
CACGACTGCT TCTGGACGCA CGCCAACACC GTGCCCGAGC TCAACCGGAT GTGCCGGGAG 
CAGTTTGTGG CCCTGCACTC GCAGCCCATT TTGGAGCAGC TCTCCGAGTT CATGCGACAT 
ACCTACAGCT TTAAGGACAG TGACTTCATC AACGATGGTT CCGTGGAGGA TCTGTCCAAG 
CGCCAGCTGA ACCGCATTCT GAAGCAGATG CCCCAGAAGG GAGACTTTGA CCTGCGCAAC 
GTGCTCGACT CAGTGTACTT CTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtRNApol ( NM_134721.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608565.1 Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13425
Clone ID Related Accession (Same CDS sequence)	NM_001272924.1 , NM_134721.3
Accession Version	NM_134721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial RNA polymerase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134721.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCGCT TGCTGAGTGC AAATCGGGCG GCGGCCGTGC AAAAAGCACT GCGAGTGCCA 
CCCACTGCTC ACCCAACAAC AACAACAGCT ACAACAACAA CAGTGTGTTG CGGCTGCGGA 
ATTGCGCCTT CAAATTTCGT GCATTATCAG CAGTATTATC AAATGCTCTA CCGCCAGATG 
CCGGCAGGAG CAATTACAGC AAAAACAACA ACGACAACCA CTCGCACACA AACAACAACA 
CCCACACAAA CGGATGCAGT GGCCCAGGCG AAAAAGAAGC GATTGCGCAA GCCGCGCTAC 
GCCAAATACG TGGAGCTCCT CGAGGTCACC GAACAAGCCA TCAGCGAGCG AAGGTCGCGT 
GTGAAGCGGC TGAAATCCAA GAAGCTGGCC GACTTCATAC ATAAGGCCCA GCAGCAAATT 
CTGGAGCGAC GCGCCCGCGA AGATCGCAAG GATAAGAAAA CCAAATGTGT ACTGGATCTG 
GCGCAGCGTC AGCCTGTAGA ATATCAGAAA ATCGATATGG AGGCTTTGGA TGGATCTATT 
CTAGAGTCTG GAGTATACGA CGATCGGCCT ATGCTCTTCT TTGGCAAGGA TGAGTACACA 
AAACAGGTGA TAGCGAATCC CGAGGAGGTG CAAAACATTC TCAAAAGCTT CCAAAACTTT 
CGGGACATCA TCGAGAGCAT GGAAAATGAT CAGCATGAAG CGGATTTTGG TCTGAGCACC 
ACAAAAGTGC ATCCTGAAGA ACACGAAATG CTGGATCCAT TCGAGGCCAT TGAGCCGTAC 
ATCAGTCCCC TGGACAAGAG CAAACGTTCA GTAACTACTG TGGCCGTGCC CAACGCCAGC 
GCAGTCACAA CCAAGTCGAA GAAACGTTTC AAGTCAAAGG TTCACGTAAA CGAGGAGCAA 
GAGCGTCTGG CCAAGCAACG GGCACTGCAC ATAAACCTGA ACACATATCT GAATGTGTGT 
GTCTCGGCCA ACATGCTAAA TCGCGCCCTG TCCACCATCA TTGCCTATCG CGGCAAGGTG 
AGGAAGAGTT CTTTGCCGCA CATGTCCGAA GGCCTCATCA CCATCGACCT GTACAACATC 
CTTCTGCACG GCTATGCGGG CAAGGGATCC TTCGATCGAG CCCAGGAGGT CTTCAAGCTC 
ATCGAAGAGG ACGGACTGTC GTTCAACGAG CAAACGTACG CAGCCATTTT CGAGTGCTTG 
GGTCGCCTGG AACCGGATGA GGAGAACCAC CAATACATAT CCGAATACGT TTCCAAAGCC 
GAAAAGGAAG GATTCAGTTT GAATCAAATT ATGGACAGGT CAAAGTTTGT GGCTGACCAG 
CGGGACATTG CTTTGGATGC GATAAGACGG CTTCGACCGG ACTTTAAGCC GGTCTATGTG 
CCACCGCAAC TCGGATACGA CAACGAACTG CTGGATCACC TCAACGAGCA TGTTATACCT 
GTTGGAGCTA AGAAAGAACC GGGAAAAGCT GACTACGCCA TAATGAACTC CAAGCGAGGC 
TATACCGCAG CTCAATTGGA ACAGCTAGCT CGGGAGCAAC TTAAAATTGA GTTGGACGGC 
AGCATTACCA TCAAGTCCAT CGAGAAATCC AAGGAGTTCG CCAACTCCGA AAAATGCCGG 
GAACTTCTAA AAGAACTGGA GGACACGTGG CGCAAACAAA TTTCGGCAGC CATTGTGCGC 
GATCTCAACA CGCTGCGTGC ACAAGTGCGC TTCAAGCCAC ACGGCTTCAT GAACTACTAC 
ACGTATCTAA AGACCCTTGA CGCCTCGCAC TTTGCGGACA TCCTGATTAA GGAGCTGTAC 
AAGCTGGCTG AGGGCTCTGA GACATTTAGT CCAACAGTGG GTCAGCTGTA CAAAGAACTC 
GGGCAGAAGG TGCAGCAACG ATACCAAATT GAGCAGAAGA AACACAACGG CACGCTGGAG 
AAGATCGGCG AGATATACAG TTCGTATTGT GAATTGTGGG GCAGCGGGAT GTCGCAGGAT 
AACACGCGTC AGGCTTGGCA GCGTCTCGTG TACGAGCAGC GGGAGAGTGG ACCAAGCATG 
GATCTGCTGG AGGTGCCGTG GCCGTCTAAT GTCCTAACAG GCGTGGGTCG ATTTTTGTAC 
AACATTCTGA TGAGGGACAT CAAGATCGAC GCCCACATAA TGAGGCAGAA GAGCAAAAGC 
AAAGCGGCCG CTCAGCCACA AAATCTGTTG CCCGCCTTTT ACACTCTTTT CCGGAATCAA 
GGACGCTTTG TTAAAGAGGA AGTCAAGCCG CATCCGGTGC TCTCGAGATT ATTCCGAGCG 
TCCCGCCAAC AGACGCTCAC GTTCGACTCC AACCTAGTGC CCATGCTGTG TCCCCCGCAA 
CCGTGGAGTA CTCCGCACAA TGGTGGCTAC CTGCTGAACA AGACTGAGCT CATTCGCCTG 
CCTCATCAAG CCATCCAGCA GTGGGACCGC ATTATGGCTT CCAACCCGCA GCACCTTTAT 
CCTGCCCTAG ATTCTCTGAA TCAGTTGGCC AGTGTGCCTT GGCGGGTGAA CACTCAGTTG 
CTGGATGTGA TTATAAAGGT GTTCCAGAAC GGAGGCGATG TGAAATTGGA CGTCCCTCAG 
CCACCCAGTT CCCTGCCGCC GCTTCCCACG TTGCCAGCTA AGGACGCCGA AGGCAATGCC 
ACCTCCAGTG AGGACCGAGC TAAGCAGTTC CGGGACAAGC TCGGACATCG AAGGAAGCAG 
GCCGAGATGT ACAGCTTGTG GTGCGATGCT CTCTATAGGT TGTCACTGGC ACAGCATTAC 
CGTGATAAGG TCTTCTGGCT GCCACACAAC ATGGACTTCC GTGGACGTGT CTATCCTGTG 
CCGCCGCATC TCAATCACCT GGGCTCGGAC CTGGCTCGTT CGATGCTCAT CTTCGATCAG 
GCGCAGCCAC TGGGAGTCGA TGGATTAAGT TGGCTTAAGC TGCACTGCAT CAATCTGACT 
GGTCTCAAGA AGCGGGACTC GGTGAGGGAA CGTCTTTTGT ACGCGGAAGA GATCATGCCA 
GATATCCTGG ACTCTGCGGA CAATCCACTC ACCGGGCGGA TGTGGTGGTC CAAATCGGAT 
GAGCCATGGC AAACGCTGGC GTGCTGCATG GAAATAGCCA ATGTTCATCG CTGTCCCGAT 
CCCGCTGCCT ATCTAAGTCG CTTCCCCATA CACCAAGACG GTTCCTGCAA TGGATTGCAG 
CACTACGCTG CCTTGGGCAG AGATGAAGCT GGTGCTCGTA GTGTCAACCT GGCACCATCG 
GCCATTCCGC AGGACGTTTA CAGTGCGGTA GCCGCTTTGG TGGAGAAGAG CAGGAAAGCG 
GATGCCCAGA ACGGTCTCCA TGTGGCGGAA GCACTGGCCG GATTCGTTCG CCGCAAGGTG 
ATCAAACAAA CAGTGATGAC AACGGTCTAC GGAGTGACTC GTTACGGAGC CAGACTGCAG 
ATAGCGCGCC AGCTAAAAGA CATTGACGAC TTCCCCAAGG ACTGGGTCTG GCCTGCGTCC 
ACATACTTGA CCACCAAGAC GTTCGAAAGC CTGCGGGAAA TGTTCACATC CACGAGAGAA 
ATTCAGGATT GGTTCACGGA GTGCGCCCGC TTGATTTCCG GCGTGTGCAG CCAGAACGTG 
GAGTGGGTCA CGCCACTCGG CCTGCCGGTG GTGCAGCCCT ACAACCGCCA GGAGATGAAG 
CACTCGCCTC GCAGCGGCTT CAAGGTCTCC GCCAACATGC CCATGGACTT GTACGAGCGG 
CCAAACATCC TCAAGCAGAA GAACGCCTTC CCGCCGAACT TCATCCACTC GCTGGACTCC 
TCGCACATGA TGCTCACCTC GCTGCACTGC GAGCGGCAGG GCATCACGTT TGTTTCGGTG 
CACGACTGCT TCTGGACGCA CGCCAACACC GTGCCCGAGC TCAACCGGAT GTGCCGGGAG 
CAGTTTGTGG CCCTGCACTC GCAGCCCATT TTGGAGCAGC TCTCCGAGTT CATGCGACAT 
ACCTACAGCT TTAAGGACAG TGACTTCATC AACGATGGTT CCGTGGAGGA TCTGTCCAAG 
CGCCAGCTGA ACCGCATTCT GAAGCAGATG CCCCAGAAGG GAGACTTTGA CCTGCGCAAC 
GTGCTCGACT CAGTGTACTT CTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608565.1
CDS	172..4281
Translation	MYRLLSANRAAAVQKALRVPPTAHPTTTTATTTTVCCGCGIAPSNFVHYQQYYQMLYRQMPAGAITAKTTTTTTRTQTTTPTQTDAVAQAKKKRLRKPRYAKYVELLEVTEQAISERRSRVKRLKSKKLADFIHKAQQQILERRAREDRKDKKTKCVLDLAQRQPVEYQKIDMEALDGSILESGVYDDRPMLFFGKDEYTKQVIANPEEVQNILKSFQNFRDIIESMENDQHEADFGLSTTKVHPEEHEMLDPFEAIEPYISPLDKSKRSVTTVAVPNASAVTTKSKKRFKSKVHVNEEQERLAKQRALHINLNTYLNVCVSANMLNRALSTIIAYRGKVRKSSLPHMSEGLITIDLYNILLHGYAGKGSFDRAQEVFKLIEEDGLSFNEQTYAAIFECLGRLEPDEENHQYISEYVSKAEKEGFSLNQIMDRSKFVADQRDIALDAIRRLRPDFKPVYVPPQLGYDNELLDHLNEHVIPVGAKKEPGKADYAIMNSKRGYTAAQLEQLAREQLKIELDGSITIKSIEKSKEFANSEKCRELLKELEDTWRKQISAAIVRDLNTLRAQVRFKPHGFMNYYTYLKTLDASHFADILIKELYKLAEGSETFSPTVGQLYKELGQKVQQRYQIEQKKHNGTLEKIGEIYSSYCELWGSGMSQDNTRQAWQRLVYEQRESGPSMDLLEVPWPSNVLTGVGRFLYNILMRDIKIDAHIMRQKSKSKAAAQPQNLLPAFYTLFRNQGRFVKEEVKPHPVLSRLFRASRQQTLTFDSNLVPMLCPPQPWSTPHNGGYLLNKTELIRLPHQAIQQWDRIMASNPQHLYPALDSLNQLASVPWRVNTQLLDVIIKVFQNGGDVKLDVPQPPSSLPPLPTLPAKDAEGNATSSEDRAKQFRDKLGHRRKQAEMYSLWCDALYRLSLAQHYRDKVFWLPHNMDFRGRVYPVPPHLNHLGSDLARSMLIFDQAQPLGVDGLSWLKLHCINLTGLKKRDSVRERLLYAEEIMPDILDSADNPLTGRMWWSKSDEPWQTLACCMEIANVHRCPDPAAYLSRFPIHQDGSCNGLQHYAALGRDEAGARSVNLAPSAIPQDVYSAVAALVEKSRKADAQNGLHVAEALAGFVRRKVIKQTVMTTVYGVTRYGARLQIARQLKDIDDFPKDWVWPASTYLTTKTFESLREMFTSTREIQDWFTECARLISGVCSQNVEWVTPLGLPVVQPYNRQEMKHSPRSGFKVSANMPMDLYERPNILKQKNAFPPNFIHSLDSSHMMLTSLHCERQGITFVSVHDCFWTHANTVPELNRMCREQFVALHSQPILEQLSEFMRHTYSFKDSDFINDGSVEDLSKRQLNRILKQMPQKGDFDLRNVLDSVYFFS

Target ORF information:

RefSeq Version	NM_134721.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial RNA polymerase (mtRNApol), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134721.3

ORF Insert Sequence:

ATGTATCGCT TGCTGAGTGC AAATCGGGCG GCGGCCGTGC AAAAAGCACT GCGAGTGCCA 
CCCACTGCTC ACCCAACAAC AACAACAGCT ACAACAACAA CAGTGTGTTG CGGCTGCGGA 
ATTGCGCCTT CAAATTTCGT GCATTATCAG CAGTATTATC AAATGCTCTA CCGCCAGATG 
CCGGCAGGAG CAATTACAGC AAAAACAACA ACGACAACCA CTCGCACACA AACAACAACA 
CCCACACAAA CGGATGCAGT GGCCCAGGCG AAAAAGAAGC GATTGCGCAA GCCGCGCTAC 
GCCAAATACG TGGAGCTCCT CGAGGTCACC GAACAAGCCA TCAGCGAGCG AAGGTCGCGT 
GTGAAGCGGC TGAAATCCAA GAAGCTGGCC GACTTCATAC ATAAGGCCCA GCAGCAAATT 
CTGGAGCGAC GCGCCCGCGA AGATCGCAAG GATAAGAAAA CCAAATGTGT ACTGGATCTG 
GCGCAGCGTC AGCCTGTAGA ATATCAGAAA ATCGATATGG AGGCTTTGGA TGGATCTATT 
CTAGAGTCTG GAGTATACGA CGATCGGCCT ATGCTCTTCT TTGGCAAGGA TGAGTACACA 
AAACAGGTGA TAGCGAATCC CGAGGAGGTG CAAAACATTC TCAAAAGCTT CCAAAACTTT 
CGGGACATCA TCGAGAGCAT GGAAAATGAT CAGCATGAAG CGGATTTTGG TCTGAGCACC 
ACAAAAGTGC ATCCTGAAGA ACACGAAATG CTGGATCCAT TCGAGGCCAT TGAGCCGTAC 
ATCAGTCCCC TGGACAAGAG CAAACGTTCA GTAACTACTG TGGCCGTGCC CAACGCCAGC 
GCAGTCACAA CCAAGTCGAA GAAACGTTTC AAGTCAAAGG TTCACGTAAA CGAGGAGCAA 
GAGCGTCTGG CCAAGCAACG GGCACTGCAC ATAAACCTGA ACACATATCT GAATGTGTGT 
GTCTCGGCCA ACATGCTAAA TCGCGCCCTG TCCACCATCA TTGCCTATCG CGGCAAGGTG 
AGGAAGAGTT CTTTGCCGCA CATGTCCGAA GGCCTCATCA CCATCGACCT GTACAACATC 
CTTCTGCACG GCTATGCGGG CAAGGGATCC TTCGATCGAG CCCAGGAGGT CTTCAAGCTC 
ATCGAAGAGG ACGGACTGTC GTTCAACGAG CAAACGTACG CAGCCATTTT CGAGTGCTTG 
GGTCGCCTGG AACCGGATGA GGAGAACCAC CAATACATAT CCGAATACGT TTCCAAAGCC 
GAAAAGGAAG GATTCAGTTT GAATCAAATT ATGGACAGGT CAAAGTTTGT GGCTGACCAG 
CGGGACATTG CTTTGGATGC GATAAGACGG CTTCGACCGG ACTTTAAGCC GGTCTATGTG 
CCACCGCAAC TCGGATACGA CAACGAACTG CTGGATCACC TCAACGAGCA TGTTATACCT 
GTTGGAGCTA AGAAAGAACC GGGAAAAGCT GACTACGCCA TAATGAACTC CAAGCGAGGC 
TATACCGCAG CTCAATTGGA ACAGCTAGCT CGGGAGCAAC TTAAAATTGA GTTGGACGGC 
AGCATTACCA TCAAGTCCAT CGAGAAATCC AAGGAGTTCG CCAACTCCGA AAAATGCCGG 
GAACTTCTAA AAGAACTGGA GGACACGTGG CGCAAACAAA TTTCGGCAGC CATTGTGCGC 
GATCTCAACA CGCTGCGTGC ACAAGTGCGC TTCAAGCCAC ACGGCTTCAT GAACTACTAC 
ACGTATCTAA AGACCCTTGA CGCCTCGCAC TTTGCGGACA TCCTGATTAA GGAGCTGTAC 
AAGCTGGCTG AGGGCTCTGA GACATTTAGT CCAACAGTGG GTCAGCTGTA CAAAGAACTC 
GGGCAGAAGG TGCAGCAACG ATACCAAATT GAGCAGAAGA AACACAACGG CACGCTGGAG 
AAGATCGGCG AGATATACAG TTCGTATTGT GAATTGTGGG GCAGCGGGAT GTCGCAGGAT 
AACACGCGTC AGGCTTGGCA GCGTCTCGTG TACGAGCAGC GGGAGAGTGG ACCAAGCATG 
GATCTGCTGG AGGTGCCGTG GCCGTCTAAT GTCCTAACAG GCGTGGGTCG ATTTTTGTAC 
AACATTCTGA TGAGGGACAT CAAGATCGAC GCCCACATAA TGAGGCAGAA GAGCAAAAGC 
AAAGCGGCCG CTCAGCCACA AAATCTGTTG CCCGCCTTTT ACACTCTTTT CCGGAATCAA 
GGACGCTTTG TTAAAGAGGA AGTCAAGCCG CATCCGGTGC TCTCGAGATT ATTCCGAGCG 
TCCCGCCAAC AGACGCTCAC GTTCGACTCC AACCTAGTGC CCATGCTGTG TCCCCCGCAA 
CCGTGGAGTA CTCCGCACAA TGGTGGCTAC CTGCTGAACA AGACTGAGCT CATTCGCCTG 
CCTCATCAAG CCATCCAGCA GTGGGACCGC ATTATGGCTT CCAACCCGCA GCACCTTTAT 
CCTGCCCTAG ATTCTCTGAA TCAGTTGGCC AGTGTGCCTT GGCGGGTGAA CACTCAGTTG 
CTGGATGTGA TTATAAAGGT GTTCCAGAAC GGAGGCGATG TGAAATTGGA CGTCCCTCAG 
CCACCCAGTT CCCTGCCGCC GCTTCCCACG TTGCCAGCTA AGGACGCCGA AGGCAATGCC 
ACCTCCAGTG AGGACCGAGC TAAGCAGTTC CGGGACAAGC TCGGACATCG AAGGAAGCAG 
GCCGAGATGT ACAGCTTGTG GTGCGATGCT CTCTATAGGT TGTCACTGGC ACAGCATTAC 
CGTGATAAGG TCTTCTGGCT GCCACACAAC ATGGACTTCC GTGGACGTGT CTATCCTGTG 
CCGCCGCATC TCAATCACCT GGGCTCGGAC CTGGCTCGTT CGATGCTCAT CTTCGATCAG 
GCGCAGCCAC TGGGAGTCGA TGGATTAAGT TGGCTTAAGC TGCACTGCAT CAATCTGACT 
GGTCTCAAGA AGCGGGACTC GGTGAGGGAA CGTCTTTTGT ACGCGGAAGA GATCATGCCA 
GATATCCTGG ACTCTGCGGA CAATCCACTC ACCGGGCGGA TGTGGTGGTC CAAATCGGAT 
GAGCCATGGC AAACGCTGGC GTGCTGCATG GAAATAGCCA ATGTTCATCG CTGTCCCGAT 
CCCGCTGCCT ATCTAAGTCG CTTCCCCATA CACCAAGACG GTTCCTGCAA TGGATTGCAG 
CACTACGCTG CCTTGGGCAG AGATGAAGCT GGTGCTCGTA GTGTCAACCT GGCACCATCG 
GCCATTCCGC AGGACGTTTA CAGTGCGGTA GCCGCTTTGG TGGAGAAGAG CAGGAAAGCG 
GATGCCCAGA ACGGTCTCCA TGTGGCGGAA GCACTGGCCG GATTCGTTCG CCGCAAGGTG 
ATCAAACAAA CAGTGATGAC AACGGTCTAC GGAGTGACTC GTTACGGAGC CAGACTGCAG 
ATAGCGCGCC AGCTAAAAGA CATTGACGAC TTCCCCAAGG ACTGGGTCTG GCCTGCGTCC 
ACATACTTGA CCACCAAGAC GTTCGAAAGC CTGCGGGAAA TGTTCACATC CACGAGAGAA 
ATTCAGGATT GGTTCACGGA GTGCGCCCGC TTGATTTCCG GCGTGTGCAG CCAGAACGTG 
GAGTGGGTCA CGCCACTCGG CCTGCCGGTG GTGCAGCCCT ACAACCGCCA GGAGATGAAG 
CACTCGCCTC GCAGCGGCTT CAAGGTCTCC GCCAACATGC CCATGGACTT GTACGAGCGG 
CCAAACATCC TCAAGCAGAA GAACGCCTTC CCGCCGAACT TCATCCACTC GCTGGACTCC 
TCGCACATGA TGCTCACCTC GCTGCACTGC GAGCGGCAGG GCATCACGTT TGTTTCGGTG 
CACGACTGCT TCTGGACGCA CGCCAACACC GTGCCCGAGC TCAACCGGAT GTGCCGGGAG 
CAGTTTGTGG CCCTGCACTC GCAGCCCATT TTGGAGCAGC TCTCCGAGTT CATGCGACAT 
ACCTACAGCT TTAAGGACAG TGACTTCATC AACGATGGTT CCGTGGAGGA TCTGTCCAAG 
CGCCAGCTGA ACCGCATTCT GAAGCAGATG CCCCAGAAGG GAGACTTTGA CCTGCGCAAC 
GTGCTCGACT CAGTGTACTT CTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mtRNApol cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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