Shh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shh
Entrez Gene ID	20423
Full Name	sonic hedgehog
Synonyms	9530036O11Rik,Dsh,Hhg1,Hx,Hxl3,M100081,ShhNC
General protein information	Preferred Names sonic hedgehog Names sonic hedgehog protein HHG-1 hemimelic extra toes shh unprocessed N-terminal signaling and C-terminal autoprocessing domains short digits
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B1\|5 14.39 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535648.3	XP_006535711.1	sonic hedgehog protein isoform X1
XM_006535649.4	XP_006535712.1	sonic hedgehog protein isoform X2
XM_006535648.4	XP_006535711.1	sonic hedgehog protein isoform X1
XM_006535649.3	XP_006535712.1	sonic hedgehog protein isoform X2
NM_009170.3	NP_033196.1	sonic hedgehog protein precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	shh	XP_002932544.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001412	XP_321721.5
Homo sapiens (human)	SHH	NP_000184.1
Mus musculus (house mouse)	Shh	NP_033196.1
Bos taurus (cattle)	SHH	XP_002687031.2
Rattus norvegicus (Norway rat)	Shh	NP_058917.1
Gallus gallus (chicken)	SHH	NP_990152.1
Danio rerio (zebrafish)	shha	NP_571138.1
Danio rerio (zebrafish)	shhb	NP_571274.2
Macaca mulatta (Rhesus monkey)	SHH	XP_001106515.2
Canis lupus familiaris (dog)	SHH	XP_850450.1
Pan troglodytes (chimpanzee)	SHH	XP_001147185.2
Drosophila melanogaster (fruit fly)	hh	NP_001034065.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple steps characterise ventricular layer attrition to form the ependymal cell lining of the adult mouse spinal cord central canal.
Cañizares MA, Albors AR, Singer G, Suttie N, Gorkic M, Felts P, Storey KG
Journal of anatomy236(2)334-350(2020 Feb)

Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration.
Schneider AJ, Gawdzik J, Vezina CM, Baker TR, Peterson RE
Gene expression patterns : GEP34119075(2019 12)

Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.
Liu C, Tate T, Batourina E, Truschel ST, Potter S, Adam M, Xiang T, Picard M, Reiley M, Schneider K, Tamargo M, Lu C, Chen X, He J, Kim H, Mendelsohn CL
Nature communications10(1)4589(2019 10)

Transcriptional control of lung alveolar type 1 cell development and maintenance by NK homeobox 2-1.
Little DR, Gerner-Mauro KN, Flodby P, Crandall ED, Borok Z, Akiyama H, Kimura S, Ostrin EJ, Chen J
Proceedings of the National Academy of Sciences of the United States of America116(41)20545-20555(2019 Oct)

Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.
Nowialis P, Lopusna K, Opavska J, Haney SL, Abraham A, Sheng P, Riva A, Natarajan A, Guryanova O, Simpson M, Hlady R, Xie M, Opavsky R
Nature communications10(1)4374(2019 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Shh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34730	XM_006535648.4 Latest version!	Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu34731	XM_006535649.4 Latest version!	Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu22903	NM_009170.3 Latest version!	Mus musculus sonic hedgehog (Shh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
View Old Accession Versions >>
OMu34730	XM_006535648.3	Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu34731	XM_006535649.3	Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shh ( XM_006535648.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535711.1 Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34730
Clone ID Related Accession (Same CDS sequence)	XM_006535648.3 , XM_006535648.4
Accession Version	XM_006535648.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sonic hedgehog protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGT CCTTGGAAAG GTGTGAGGAG AGGTGCAAAG ACAAGTTAAA TGCCTTGGCC 
ATCTCTGTGA TGAACCAGTG GCCTGGAGTG AAGCTGCGAG TGACCGAGGG CTGGGATGAG 
GACGGCCATC ATTCAGAGGA GTCTCTACAC TATGAGGGTC GAGCAGTGGA CATCACCACG 
TCCGACCGGG ACCGCAGCAA GTACGGCATG CTGGCTCGCC TGGCTGTGGA AGCAGGTTTC 
GACTGGGTCT ACTATGAATC CAAAGCTCAC ATCCACTGTT CTGTGAAAGC AGAGAACTCC 
GTGGCGGCCA AATCCGGCGG CTGTTTCCCG GGATCCGCCA CCGTGCACCT GGAGCAGGGC 
GGCACCAAGC TGGTGAAGGA CTTACGTCCC GGAGACCGCG TGCTGGCGGC TGACGACCAG 
GGCCGGCTGC TGTACAGCGA CTTCCTCACC TTCCTGGACC GCGACGAAGG CGCCAAGAAG 
GTCTTCTACG TGATCGAGAC GCTGGAGCCG CGCGAGCGCC TGCTGCTCAC CGCCGCGCAC 
CTGCTCTTCG TGGCGCCGCA CAACGACTCG GGGCCCACGC CCGGGCCAAG CGCGCTCTTT 
GCCAGCCGCG TGCGCCCCGG GCAGCGCGTG TACGTGGTGG CTGAACGCGG CGGGGACCGC 
CGGCTGCTGC CCGCCGCGGT GCACAGCGTG ACGCTGCGAG AGGAGGAGGC GGGCGCGTAC 
GCGCCGCTCA CGGCGCACGG CACCATTCTC ATCAACCGGG TGCTCGCCTC GTGCTACGCT 
GTCATCGAGG AGCACAGCTG GGCACACCGG GCCTTCGCGC CTTTCCGCCT GGCGCACGCG 
CTGCTGGCCG CGCTGGCACC CGCCCGCACG GACGGCGGGG GCGGGGGCAG CATCCCTGCA 
GCGCAATCTG CAACGGAAGC GAGGGGCGCG GAGCCGACTG CGGGCATCCA CTGGTACTCG 
CAGCTGCTCT ACCACATTGG CACCTGGCTG TTGGACAGCG AGACCATGCA TCCCTTGGGA 
ATGGCGGTCA AGTCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535711.1
CDS	16..1056
Translation	MRRSLERCEERCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Target ORF information:

RefSeq Version	XM_006535648.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535648.3

ORF Insert Sequence:

ATGAGGAGGT CCTTGGAAAG GTGTGAGGAG AGGTGCAAAG ACAAGTTAAA TGCCTTGGCC 
ATCTCTGTGA TGAACCAGTG GCCTGGAGTG AAGCTGCGAG TGACCGAGGG CTGGGATGAG 
GACGGCCATC ATTCAGAGGA GTCTCTACAC TATGAGGGTC GAGCAGTGGA CATCACCACG 
TCCGACCGGG ACCGCAGCAA GTACGGCATG CTGGCTCGCC TGGCTGTGGA AGCAGGTTTC 
GACTGGGTCT ACTATGAATC CAAAGCTCAC ATCCACTGTT CTGTGAAAGC AGAGAACTCC 
GTGGCGGCCA AATCCGGCGG CTGTTTCCCG GGATCCGCCA CCGTGCACCT GGAGCAGGGC 
GGCACCAAGC TGGTGAAGGA CTTACGTCCC GGAGACCGCG TGCTGGCGGC TGACGACCAG 
GGCCGGCTGC TGTACAGCGA CTTCCTCACC TTCCTGGACC GCGACGAAGG CGCCAAGAAG 
GTCTTCTACG TGATCGAGAC GCTGGAGCCG CGCGAGCGCC TGCTGCTCAC CGCCGCGCAC 
CTGCTCTTCG TGGCGCCGCA CAACGACTCG GGGCCCACGC CCGGGCCAAG CGCGCTCTTT 
GCCAGCCGCG TGCGCCCCGG GCAGCGCGTG TACGTGGTGG CTGAACGCGG CGGGGACCGC 
CGGCTGCTGC CCGCCGCGGT GCACAGCGTG ACGCTGCGAG AGGAGGAGGC GGGCGCGTAC 
GCGCCGCTCA CGGCGCACGG CACCATTCTC ATCAACCGGG TGCTCGCCTC GTGCTACGCT 
GTCATCGAGG AGCACAGCTG GGCACACCGG GCCTTCGCGC CTTTCCGCCT GGCGCACGCG 
CTGCTGGCCG CGCTGGCACC CGCCCGCACG GACGGCGGGG GCGGGGGCAG CATCCCTGCA 
GCGCAATCTG CAACGGAAGC GAGGGGCGCG GAGCCGACTG CGGGCATCCA CTGGTACTCG 
CAGCTGCTCT ACCACATTGG CACCTGGCTG TTGGACAGCG AGACCATGCA TCCCTTGGGA 
ATGGCGGTCA AGTCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shh ( XM_006535648.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535711.1 Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34730
Clone ID Related Accession (Same CDS sequence)	XM_006535648.3 , XM_006535648.4
Accession Version	XM_006535648.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sonic hedgehog protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535648.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGT CCTTGGAAAG GTGTGAGGAG AGGTGCAAAG ACAAGTTAAA TGCCTTGGCC 
ATCTCTGTGA TGAACCAGTG GCCTGGAGTG AAGCTGCGAG TGACCGAGGG CTGGGATGAG 
GACGGCCATC ATTCAGAGGA GTCTCTACAC TATGAGGGTC GAGCAGTGGA CATCACCACG 
TCCGACCGGG ACCGCAGCAA GTACGGCATG CTGGCTCGCC TGGCTGTGGA AGCAGGTTTC 
GACTGGGTCT ACTATGAATC CAAAGCTCAC ATCCACTGTT CTGTGAAAGC AGAGAACTCC 
GTGGCGGCCA AATCCGGCGG CTGTTTCCCG GGATCCGCCA CCGTGCACCT GGAGCAGGGC 
GGCACCAAGC TGGTGAAGGA CTTACGTCCC GGAGACCGCG TGCTGGCGGC TGACGACCAG 
GGCCGGCTGC TGTACAGCGA CTTCCTCACC TTCCTGGACC GCGACGAAGG CGCCAAGAAG 
GTCTTCTACG TGATCGAGAC GCTGGAGCCG CGCGAGCGCC TGCTGCTCAC CGCCGCGCAC 
CTGCTCTTCG TGGCGCCGCA CAACGACTCG GGGCCCACGC CCGGGCCAAG CGCGCTCTTT 
GCCAGCCGCG TGCGCCCCGG GCAGCGCGTG TACGTGGTGG CTGAACGCGG CGGGGACCGC 
CGGCTGCTGC CCGCCGCGGT GCACAGCGTG ACGCTGCGAG AGGAGGAGGC GGGCGCGTAC 
GCGCCGCTCA CGGCGCACGG CACCATTCTC ATCAACCGGG TGCTCGCCTC GTGCTACGCT 
GTCATCGAGG AGCACAGCTG GGCACACCGG GCCTTCGCGC CTTTCCGCCT GGCGCACGCG 
CTGCTGGCCG CGCTGGCACC CGCCCGCACG GACGGCGGGG GCGGGGGCAG CATCCCTGCA 
GCGCAATCTG CAACGGAAGC GAGGGGCGCG GAGCCGACTG CGGGCATCCA CTGGTACTCG 
CAGCTGCTCT ACCACATTGG CACCTGGCTG TTGGACAGCG AGACCATGCA TCCCTTGGGA 
ATGGCGGTCA AGTCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535711.1
CDS	145..1185
Translation	MRRSLERCEERCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Target ORF information:

RefSeq Version	XM_006535648.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sonic hedgehog (Shh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535648.4

ORF Insert Sequence:

ATGAGGAGGT CCTTGGAAAG GTGTGAGGAG AGGTGCAAAG ACAAGTTAAA TGCCTTGGCC 
ATCTCTGTGA TGAACCAGTG GCCTGGAGTG AAGCTGCGAG TGACCGAGGG CTGGGATGAG 
GACGGCCATC ATTCAGAGGA GTCTCTACAC TATGAGGGTC GAGCAGTGGA CATCACCACG 
TCCGACCGGG ACCGCAGCAA GTACGGCATG CTGGCTCGCC TGGCTGTGGA AGCAGGTTTC 
GACTGGGTCT ACTATGAATC CAAAGCTCAC ATCCACTGTT CTGTGAAAGC AGAGAACTCC 
GTGGCGGCCA AATCCGGCGG CTGTTTCCCG GGATCCGCCA CCGTGCACCT GGAGCAGGGC 
GGCACCAAGC TGGTGAAGGA CTTACGTCCC GGAGACCGCG TGCTGGCGGC TGACGACCAG 
GGCCGGCTGC TGTACAGCGA CTTCCTCACC TTCCTGGACC GCGACGAAGG CGCCAAGAAG 
GTCTTCTACG TGATCGAGAC GCTGGAGCCG CGCGAGCGCC TGCTGCTCAC CGCCGCGCAC 
CTGCTCTTCG TGGCGCCGCA CAACGACTCG GGGCCCACGC CCGGGCCAAG CGCGCTCTTT 
GCCAGCCGCG TGCGCCCCGG GCAGCGCGTG TACGTGGTGG CTGAACGCGG CGGGGACCGC 
CGGCTGCTGC CCGCCGCGGT GCACAGCGTG ACGCTGCGAG AGGAGGAGGC GGGCGCGTAC 
GCGCCGCTCA CGGCGCACGG CACCATTCTC ATCAACCGGG TGCTCGCCTC GTGCTACGCT 
GTCATCGAGG AGCACAGCTG GGCACACCGG GCCTTCGCGC CTTTCCGCCT GGCGCACGCG 
CTGCTGGCCG CGCTGGCACC CGCCCGCACG GACGGCGGGG GCGGGGGCAG CATCCCTGCA 
GCGCAATCTG CAACGGAAGC GAGGGGCGCG GAGCCGACTG CGGGCATCCA CTGGTACTCG 
CAGCTGCTCT ACCACATTGG CACCTGGCTG TTGGACAGCG AGACCATGCA TCCCTTGGGA 
ATGGCGGTCA AGTCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shh ( XM_006535649.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535712.1 Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34731
Clone ID Related Accession (Same CDS sequence)	XM_006535649.4 , XM_006535649.3
Accession Version	XM_006535649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sonic hedgehog protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGT GGCCTGGAGT GAAGCTGCGA GTGACCGAGG GCTGGGATGA GGACGGCCAT 
CATTCAGAGG AGTCTCTACA CTATGAGGGT CGAGCAGTGG ACATCACCAC GTCCGACCGG 
GACCGCAGCA AGTACGGCAT GCTGGCTCGC CTGGCTGTGG AAGCAGGTTT CGACTGGGTC 
TACTATGAAT CCAAAGCTCA CATCCACTGT TCTGTGAAAG CAGAGAACTC CGTGGCGGCC 
AAATCCGGCG GCTGTTTCCC GGGATCCGCC ACCGTGCACC TGGAGCAGGG CGGCACCAAG 
CTGGTGAAGG ACTTACGTCC CGGAGACCGC GTGCTGGCGG CTGACGACCA GGGCCGGCTG 
CTGTACAGCG ACTTCCTCAC CTTCCTGGAC CGCGACGAAG GCGCCAAGAA GGTCTTCTAC 
GTGATCGAGA CGCTGGAGCC GCGCGAGCGC CTGCTGCTCA CCGCCGCGCA CCTGCTCTTC 
GTGGCGCCGC ACAACGACTC GGGGCCCACG CCCGGGCCAA GCGCGCTCTT TGCCAGCCGC 
GTGCGCCCCG GGCAGCGCGT GTACGTGGTG GCTGAACGCG GCGGGGACCG CCGGCTGCTG 
CCCGCCGCGG TGCACAGCGT GACGCTGCGA GAGGAGGAGG CGGGCGCGTA CGCGCCGCTC 
ACGGCGCACG GCACCATTCT CATCAACCGG GTGCTCGCCT CGTGCTACGC TGTCATCGAG 
GAGCACAGCT GGGCACACCG GGCCTTCGCG CCTTTCCGCC TGGCGCACGC GCTGCTGGCC 
GCGCTGGCAC CCGCCCGCAC GGACGGCGGG GGCGGGGGCA GCATCCCTGC AGCGCAATCT 
GCAACGGAAG CGAGGGGCGC GGAGCCGACT GCGGGCATCC ACTGGTACTC GCAGCTGCTC 
TACCACATTG GCACCTGGCT GTTGGACAGC GAGACCATGC ATCCCTTGGG AATGGCGGTC 
AAGTCCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535712.1
CDS	64..1035
Translation	MNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Target ORF information:

RefSeq Version	XM_006535649.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535649.4

ORF Insert Sequence:

ATGAACCAGT GGCCTGGAGT GAAGCTGCGA GTGACCGAGG GCTGGGATGA GGACGGCCAT 
CATTCAGAGG AGTCTCTACA CTATGAGGGT CGAGCAGTGG ACATCACCAC GTCCGACCGG 
GACCGCAGCA AGTACGGCAT GCTGGCTCGC CTGGCTGTGG AAGCAGGTTT CGACTGGGTC 
TACTATGAAT CCAAAGCTCA CATCCACTGT TCTGTGAAAG CAGAGAACTC CGTGGCGGCC 
AAATCCGGCG GCTGTTTCCC GGGATCCGCC ACCGTGCACC TGGAGCAGGG CGGCACCAAG 
CTGGTGAAGG ACTTACGTCC CGGAGACCGC GTGCTGGCGG CTGACGACCA GGGCCGGCTG 
CTGTACAGCG ACTTCCTCAC CTTCCTGGAC CGCGACGAAG GCGCCAAGAA GGTCTTCTAC 
GTGATCGAGA CGCTGGAGCC GCGCGAGCGC CTGCTGCTCA CCGCCGCGCA CCTGCTCTTC 
GTGGCGCCGC ACAACGACTC GGGGCCCACG CCCGGGCCAA GCGCGCTCTT TGCCAGCCGC 
GTGCGCCCCG GGCAGCGCGT GTACGTGGTG GCTGAACGCG GCGGGGACCG CCGGCTGCTG 
CCCGCCGCGG TGCACAGCGT GACGCTGCGA GAGGAGGAGG CGGGCGCGTA CGCGCCGCTC 
ACGGCGCACG GCACCATTCT CATCAACCGG GTGCTCGCCT CGTGCTACGC TGTCATCGAG 
GAGCACAGCT GGGCACACCG GGCCTTCGCG CCTTTCCGCC TGGCGCACGC GCTGCTGGCC 
GCGCTGGCAC CCGCCCGCAC GGACGGCGGG GGCGGGGGCA GCATCCCTGC AGCGCAATCT 
GCAACGGAAG CGAGGGGCGC GGAGCCGACT GCGGGCATCC ACTGGTACTC GCAGCTGCTC 
TACCACATTG GCACCTGGCT GTTGGACAGC GAGACCATGC ATCCCTTGGG AATGGCGGTC 
AAGTCCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shh ( XM_006535649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535712.1 Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34731
Clone ID Related Accession (Same CDS sequence)	XM_006535649.4 , XM_006535649.3
Accession Version	XM_006535649.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sonic hedgehog protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGT GGCCTGGAGT GAAGCTGCGA GTGACCGAGG GCTGGGATGA GGACGGCCAT 
CATTCAGAGG AGTCTCTACA CTATGAGGGT CGAGCAGTGG ACATCACCAC GTCCGACCGG 
GACCGCAGCA AGTACGGCAT GCTGGCTCGC CTGGCTGTGG AAGCAGGTTT CGACTGGGTC 
TACTATGAAT CCAAAGCTCA CATCCACTGT TCTGTGAAAG CAGAGAACTC CGTGGCGGCC 
AAATCCGGCG GCTGTTTCCC GGGATCCGCC ACCGTGCACC TGGAGCAGGG CGGCACCAAG 
CTGGTGAAGG ACTTACGTCC CGGAGACCGC GTGCTGGCGG CTGACGACCA GGGCCGGCTG 
CTGTACAGCG ACTTCCTCAC CTTCCTGGAC CGCGACGAAG GCGCCAAGAA GGTCTTCTAC 
GTGATCGAGA CGCTGGAGCC GCGCGAGCGC CTGCTGCTCA CCGCCGCGCA CCTGCTCTTC 
GTGGCGCCGC ACAACGACTC GGGGCCCACG CCCGGGCCAA GCGCGCTCTT TGCCAGCCGC 
GTGCGCCCCG GGCAGCGCGT GTACGTGGTG GCTGAACGCG GCGGGGACCG CCGGCTGCTG 
CCCGCCGCGG TGCACAGCGT GACGCTGCGA GAGGAGGAGG CGGGCGCGTA CGCGCCGCTC 
ACGGCGCACG GCACCATTCT CATCAACCGG GTGCTCGCCT CGTGCTACGC TGTCATCGAG 
GAGCACAGCT GGGCACACCG GGCCTTCGCG CCTTTCCGCC TGGCGCACGC GCTGCTGGCC 
GCGCTGGCAC CCGCCCGCAC GGACGGCGGG GGCGGGGGCA GCATCCCTGC AGCGCAATCT 
GCAACGGAAG CGAGGGGCGC GGAGCCGACT GCGGGCATCC ACTGGTACTC GCAGCTGCTC 
TACCACATTG GCACCTGGCT GTTGGACAGC GAGACCATGC ATCCCTTGGG AATGGCGGTC 
AAGTCCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535712.1
CDS	130..1101
Translation	MNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Target ORF information:

RefSeq Version	XM_006535649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sonic hedgehog (Shh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535649.3

ORF Insert Sequence:

ATGAACCAGT GGCCTGGAGT GAAGCTGCGA GTGACCGAGG GCTGGGATGA GGACGGCCAT 
CATTCAGAGG AGTCTCTACA CTATGAGGGT CGAGCAGTGG ACATCACCAC GTCCGACCGG 
GACCGCAGCA AGTACGGCAT GCTGGCTCGC CTGGCTGTGG AAGCAGGTTT CGACTGGGTC 
TACTATGAAT CCAAAGCTCA CATCCACTGT TCTGTGAAAG CAGAGAACTC CGTGGCGGCC 
AAATCCGGCG GCTGTTTCCC GGGATCCGCC ACCGTGCACC TGGAGCAGGG CGGCACCAAG 
CTGGTGAAGG ACTTACGTCC CGGAGACCGC GTGCTGGCGG CTGACGACCA GGGCCGGCTG 
CTGTACAGCG ACTTCCTCAC CTTCCTGGAC CGCGACGAAG GCGCCAAGAA GGTCTTCTAC 
GTGATCGAGA CGCTGGAGCC GCGCGAGCGC CTGCTGCTCA CCGCCGCGCA CCTGCTCTTC 
GTGGCGCCGC ACAACGACTC GGGGCCCACG CCCGGGCCAA GCGCGCTCTT TGCCAGCCGC 
GTGCGCCCCG GGCAGCGCGT GTACGTGGTG GCTGAACGCG GCGGGGACCG CCGGCTGCTG 
CCCGCCGCGG TGCACAGCGT GACGCTGCGA GAGGAGGAGG CGGGCGCGTA CGCGCCGCTC 
ACGGCGCACG GCACCATTCT CATCAACCGG GTGCTCGCCT CGTGCTACGC TGTCATCGAG 
GAGCACAGCT GGGCACACCG GGCCTTCGCG CCTTTCCGCC TGGCGCACGC GCTGCTGGCC 
GCGCTGGCAC CCGCCCGCAC GGACGGCGGG GGCGGGGGCA GCATCCCTGC AGCGCAATCT 
GCAACGGAAG CGAGGGGCGC GGAGCCGACT GCGGGCATCC ACTGGTACTC GCAGCTGCTC 
TACCACATTG GCACCTGGCT GTTGGACAGC GAGACCATGC ATCCCTTGGG AATGGCGGTC 
AAGTCCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shh ( NM_009170.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033196.1 Mus musculus sonic hedgehog (Shh), mRNA.

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CloneID	OMu22903
Clone ID Related Accession (Same CDS sequence)	NM_009170.3
Accession Version	NM_009170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	sonic hedgehog protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077688.1 and BC063087.1. On Dec 1, 2007 this sequence version replaced NM_009170.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063087.1, AK052447.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381, SAMN01164133 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTGC TGCTGGCCAG ATGTTTTCTG GTGATCCTTG CTTCCTCGCT GCTGGTGTGC 
CCCGGGCTGG CCTGTGGGCC CGGCAGGGGG TTTGGAAAGA GGCGGCACCC CAAAAAGCTG 
ACCCCTTTAG CCTACAAGCA GTTTATTCCC AACGTAGCCG AGAAGACCCT AGGGGCCAGC 
GGCAGATATG AAGGGAAGAT CACAAGAAAC TCCGAACGAT TTAAGGAACT CACCCCCAAT 
TACAACCCCG ACATCATATT TAAGGATGAG GAAAACACGG GAGCAGACCG GCTGATGACT 
CAGAGGTGCA AAGACAAGTT AAATGCCTTG GCCATCTCTG TGATGAACCA GTGGCCTGGA 
GTGAAGCTGC GAGTGACCGA GGGCTGGGAT GAGGACGGCC ATCATTCAGA GGAGTCTCTA 
CACTATGAGG GTCGAGCAGT GGACATCACC ACGTCCGACC GGGACCGCAG CAAGTACGGC 
ATGCTGGCTC GCCTGGCTGT GGAAGCAGGT TTCGACTGGG TCTACTATGA ATCCAAAGCT 
CACATCCACT GTTCTGTGAA AGCAGAGAAC TCCGTGGCGG CCAAATCCGG CGGCTGTTTC 
CCGGGATCCG CCACCGTGCA CCTGGAGCAG GGCGGCACCA AGCTGGTGAA GGACTTACGT 
CCCGGAGACC GCGTGCTGGC GGCTGACGAC CAGGGCCGGC TGCTGTACAG CGACTTCCTC 
ACCTTCCTGG ACCGCGACGA AGGCGCCAAG AAGGTCTTCT ACGTGATCGA GACGCTGGAG 
CCGCGCGAGC GCCTGCTGCT CACCGCCGCG CACCTGCTCT TCGTGGCGCC GCACAACGAC 
TCGGGGCCCA CGCCCGGGCC AAGCGCGCTC TTTGCCAGCC GCGTGCGCCC CGGGCAGCGC 
GTGTACGTGG TGGCTGAACG CGGCGGGGAC CGCCGGCTGC TGCCCGCCGC GGTGCACAGC 
GTGACGCTGC GAGAGGAGGA GGCGGGCGCG TACGCGCCGC TCACGGCGCA CGGCACCATT 
CTCATCAACC GGGTGCTCGC CTCGTGCTAC GCTGTCATCG AGGAGCACAG CTGGGCACAC 
CGGGCCTTCG CGCCTTTCCG CCTGGCGCAC GCGCTGCTGG CCGCGCTGGC ACCCGCCCGC 
ACGGACGGCG GGGGCGGGGG CAGCATCCCT GCAGCGCAAT CTGCAACGGA AGCGAGGGGC 
GCGGAGCCGA CTGCGGGCAT CCACTGGTAC TCGCAGCTGC TCTACCACAT TGGCACCTGG 
CTGTTGGACA GCGAGACCAT GCATCCCTTG GGAATGGCGG TCAAGTCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033196.1
CDS	339..1652
Translation	MLLLLARCFLVILASSLLVCPGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Target ORF information:

RefSeq Version	NM_009170.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sonic hedgehog (Shh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009170.3

ORF Insert Sequence:

ATGCTGCTGC TGCTGGCCAG ATGTTTTCTG GTGATCCTTG CTTCCTCGCT GCTGGTGTGC 
CCCGGGCTGG CCTGTGGGCC CGGCAGGGGG TTTGGAAAGA GGCGGCACCC CAAAAAGCTG 
ACCCCTTTAG CCTACAAGCA GTTTATTCCC AACGTAGCCG AGAAGACCCT AGGGGCCAGC 
GGCAGATATG AAGGGAAGAT CACAAGAAAC TCCGAACGAT TTAAGGAACT CACCCCCAAT 
TACAACCCCG ACATCATATT TAAGGATGAG GAAAACACGG GAGCAGACCG GCTGATGACT 
CAGAGGTGCA AAGACAAGTT AAATGCCTTG GCCATCTCTG TGATGAACCA GTGGCCTGGA 
GTGAAGCTGC GAGTGACCGA GGGCTGGGAT GAGGACGGCC ATCATTCAGA GGAGTCTCTA 
CACTATGAGG GTCGAGCAGT GGACATCACC ACGTCCGACC GGGACCGCAG CAAGTACGGC 
ATGCTGGCTC GCCTGGCTGT GGAAGCAGGT TTCGACTGGG TCTACTATGA ATCCAAAGCT 
CACATCCACT GTTCTGTGAA AGCAGAGAAC TCCGTGGCGG CCAAATCCGG CGGCTGTTTC 
CCGGGATCCG CCACCGTGCA CCTGGAGCAG GGCGGCACCA AGCTGGTGAA GGACTTACGT 
CCCGGAGACC GCGTGCTGGC GGCTGACGAC CAGGGCCGGC TGCTGTACAG CGACTTCCTC 
ACCTTCCTGG ACCGCGACGA AGGCGCCAAG AAGGTCTTCT ACGTGATCGA GACGCTGGAG 
CCGCGCGAGC GCCTGCTGCT CACCGCCGCG CACCTGCTCT TCGTGGCGCC GCACAACGAC 
TCGGGGCCCA CGCCCGGGCC AAGCGCGCTC TTTGCCAGCC GCGTGCGCCC CGGGCAGCGC 
GTGTACGTGG TGGCTGAACG CGGCGGGGAC CGCCGGCTGC TGCCCGCCGC GGTGCACAGC 
GTGACGCTGC GAGAGGAGGA GGCGGGCGCG TACGCGCCGC TCACGGCGCA CGGCACCATT 
CTCATCAACC GGGTGCTCGC CTCGTGCTAC GCTGTCATCG AGGAGCACAG CTGGGCACAC 
CGGGCCTTCG CGCCTTTCCG CCTGGCGCAC GCGCTGCTGG CCGCGCTGGC ACCCGCCCGC 
ACGGACGGCG GGGGCGGGGG CAGCATCCCT GCAGCGCAAT CTGCAACGGA AGCGAGGGGC 
GCGGAGCCGA CTGCGGGCAT CCACTGGTAC TCGCAGCTGC TCTACCACAT TGGCACCTGG 
CTGTTGGACA GCGAGACCAT GCATCCCTTG GGAATGGCGG TCAAGTCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple steps characterise ventricular layer attrition to form the ependymal cell lining of the adult mouse spinal cord central canal.
Journal of anatomy236(2)334-350(2020 Feb)
Cañizares MA,Albors AR,Singer G,Suttie N,Gorkic M,Felts P,Storey KG

Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration.
Gene expression patterns : GEP34119075(2019 12)
Schneider AJ,Gawdzik J,Vezina CM,Baker TR,Peterson RE

Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells.
Nature communications10(1)4589(2019 10)
Liu C,Tate T,Batourina E,Truschel ST,Potter S,Adam M,Xiang T,Picard M,Reiley M,Schneider K,Tamargo M,Lu C,Chen X,He J,Kim H,Mendelsohn CL

Transcriptional control of lung alveolar type 1 cell development and maintenance by NK homeobox 2-1.
Proceedings of the National Academy of Sciences of the United States of America116(41)20545-20555(2019 Oct)
Little DR,Gerner-Mauro KN,Flodby P,Crandall ED,Borok Z,Akiyama H,Kimura S,Ostrin EJ,Chen J

Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.
Nature communications10(1)4374(2019 09)
Nowialis P,Lopusna K,Opavska J,Haney SL,Abraham A,Sheng P,Riva A,Natarajan A,Guryanova O,Simpson M,Hlady R,Xie M,Opavsky R

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Shh cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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