hh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hh
Entrez Gene ID	42737
Full Name	hedgehog
Synonyms	CG4637,Dmel\CG4637,HH,Hg,Hh,Mir,Mrt,anon-WO0134654.19,anon-WO0182946.19,bar,bar-3,bar3,hg,l(3)hh,l(3)neo56,l(3)neo57
General protein information	Preferred Names hedgehog Names CG4637 gene product from transcript CG4637-RB CG4637-PB PKC atypical bar-on-3 hedghog hh-PB mirabile moonrat
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E1-94E1\|3-81.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001038976.1	NP_001034065.1	hedgehog

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	shh	XP_002932544.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001412	XP_321721.5
Homo sapiens (human)	SHH	NP_000184.1
Mus musculus (house mouse)	Shh	NP_033196.1
Bos taurus (cattle)	SHH	XP_002687031.2
Rattus norvegicus (Norway rat)	Shh	NP_058917.1
Gallus gallus (chicken)	SHH	NP_990152.1
Danio rerio (zebrafish)	shha	NP_571138.1
Danio rerio (zebrafish)	shhb	NP_571274.2
Macaca mulatta (Rhesus monkey)	SHH	XP_001106515.2
Canis lupus familiaris (dog)	SHH	XP_850450.1
Pan troglodytes (chimpanzee)	SHH	XP_001147185.2
Drosophila melanogaster (fruit fly)	hh	NP_001034065.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05754	NM_001038976.1 Latest version!	Drosophila melanogaster hedgehog (hh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hh ( NM_001038976.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001034065.1 Drosophila melanogaster hedgehog (hh), mRNA.

Price & Availability↑

CloneID	OFa05754
Clone ID Related Accession (Same CDS sequence)	NM_001038976.1
Accession Version	NM_001038976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	hedgehog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACC ACAGCTCAGT GCCTTGGGCC AGTGCCGCCA GTGTCACCTG TCTCTCCCTG 
GATGCCAAAT GCCACAGTTC CAGTTCCAGT TCCAGCTCCA AATCCGCAGC GAGCTCCATC 
TCCGCAATCC CGCAAGAAGA AACGCAAACG ATGCGCCACA TTGCGCATAC GCAGCGTTGC 
CTCAGCAGGC TGACCTCTCT GGTGGCCCTG CTGCTGATCG TCTTGCCGAT GGTCTTTAGC 
CCGGCTCACA GCTGCGGTCC TGGCCGAGGA TTGGGTCGTC ATAGGGCGCG CAACCTGTAT 
CCGCTGGTCC TCAAGCAGAC AATTCCCAAT CTATCCGAGT ACACGAACAG CGCCTCCGGA 
CCTCTGGAGG GTGTGATCCG TCGGGACTCG CCCAAATTCA AGGACCTCGT GCCCAACTAC 
AACAGGGACA TCCTTTTCCG CGACGAGGAA GGCACCGGAG CGGATCGCTT GATGAGCAAG 
CGCTGCAAGG AGAAGCTAAA CGTGCTGGCC TACTCGGTGA TGAACGAATG GCCCGGCATC 
CGGCTGCTGG TCACCGAGAG CTGGGACGAG GACTACCATC ACGGCCAGGA GTCGCTCCAC 
TACGAGGGCC GAGCGGTGAC CATTGCCACC TCCGATCGCG ACCAGTCCAA ATACGGCATG 
CTCGCTCGCC TGGCCGTCGA GGCTGGATTC GATTGGGTCT CCTACGTCAG CAGGCGCCAC 
ATCTACTGCT CCGTCAAGTC AGATTCGTCG ATCAGTTCCC ACGTGCACGG CTGCTTCACG 
CCGGAGAGCA CAGCGCTGCT GGAGAGTGGA GTCCGGAAGC CGCTCGGCGA GCTCTCTATC 
GGAGATCGTG TTTTGAGCAT GACCGCCAAC GGACAGGCCG TCTACAGCGA AGTGATCCTC 
TTCATGGACC GCAACCTCGA GCAGATGCAA AACTTTGTGC AGCTGCACAC GGACGGTGGA 
GCAGTGCTCA CGGTGACGCC GGCTCACCTG GTTAGCGTTT GGCAGCCGGA GAGCCAGAAG 
CTCACGTTTG TGTTTGCGGA TCGCATCGAG GAGAAGAACC AGGTGCTCGT ACGGGATGTG 
GAGACGGGCG AGCTGAGGCC CCAGCGAGTC GTCAAGGTGG GCAGTGTGCG CAGTAAGGGC 
GTGGTCGCGC CGCTGACCCG CGAGGGCACC ATTGTGGTCA ACTCGGTGGC CGCCAGTTGC 
TATGCGGTGA TCAACAGCCA GTCGCTGGCC CACTGGGGAC TGGCTCCCAT GCGCCTGCTG 
TCCACGCTGG AGGCGTGGCT GCCCGCCAAG GAGCAGTTGC ACAGTTCGCC GAAGGTGGTG 
AGCTCGGCGC AGCAGCAGAA TGGCATCCAT TGGTATGCCA ATGCGCTCTA CAAGGTCAAG 
GACTACGTTC TGCCGCAGAG CTGGCGCCAC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034065.1
CDS	386..1801
Translation	MDNHSSVPWASAASVTCLSLDAKCHSSSSSSSSKSAASSISAIPQEETQTMRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGGAVLTVTPAHLVSVWQPESQKLTFVFADRIEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVSSAQQQNGIHWYANALYKVKDYVLPQSWRHD

Target ORF information:

RefSeq Version	NM_001038976.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hedgehog (hh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038976.1

ORF Insert Sequence:

ATGGATAACC ACAGCTCAGT GCCTTGGGCC AGTGCCGCCA GTGTCACCTG TCTCTCCCTG 
GATGCCAAAT GCCACAGTTC CAGTTCCAGT TCCAGCTCCA AATCCGCAGC GAGCTCCATC 
TCCGCAATCC CGCAAGAAGA AACGCAAACG ATGCGCCACA TTGCGCATAC GCAGCGTTGC 
CTCAGCAGGC TGACCTCTCT GGTGGCCCTG CTGCTGATCG TCTTGCCGAT GGTCTTTAGC 
CCGGCTCACA GCTGCGGTCC TGGCCGAGGA TTGGGTCGTC ATAGGGCGCG CAACCTGTAT 
CCGCTGGTCC TCAAGCAGAC AATTCCCAAT CTATCCGAGT ACACGAACAG CGCCTCCGGA 
CCTCTGGAGG GTGTGATCCG TCGGGACTCG CCCAAATTCA AGGACCTCGT GCCCAACTAC 
AACAGGGACA TCCTTTTCCG CGACGAGGAA GGCACCGGAG CGGATCGCTT GATGAGCAAG 
CGCTGCAAGG AGAAGCTAAA CGTGCTGGCC TACTCGGTGA TGAACGAATG GCCCGGCATC 
CGGCTGCTGG TCACCGAGAG CTGGGACGAG GACTACCATC ACGGCCAGGA GTCGCTCCAC 
TACGAGGGCC GAGCGGTGAC CATTGCCACC TCCGATCGCG ACCAGTCCAA ATACGGCATG 
CTCGCTCGCC TGGCCGTCGA GGCTGGATTC GATTGGGTCT CCTACGTCAG CAGGCGCCAC 
ATCTACTGCT CCGTCAAGTC AGATTCGTCG ATCAGTTCCC ACGTGCACGG CTGCTTCACG 
CCGGAGAGCA CAGCGCTGCT GGAGAGTGGA GTCCGGAAGC CGCTCGGCGA GCTCTCTATC 
GGAGATCGTG TTTTGAGCAT GACCGCCAAC GGACAGGCCG TCTACAGCGA AGTGATCCTC 
TTCATGGACC GCAACCTCGA GCAGATGCAA AACTTTGTGC AGCTGCACAC GGACGGTGGA 
GCAGTGCTCA CGGTGACGCC GGCTCACCTG GTTAGCGTTT GGCAGCCGGA GAGCCAGAAG 
CTCACGTTTG TGTTTGCGGA TCGCATCGAG GAGAAGAACC AGGTGCTCGT ACGGGATGTG 
GAGACGGGCG AGCTGAGGCC CCAGCGAGTC GTCAAGGTGG GCAGTGTGCG CAGTAAGGGC 
GTGGTCGCGC CGCTGACCCG CGAGGGCACC ATTGTGGTCA ACTCGGTGGC CGCCAGTTGC 
TATGCGGTGA TCAACAGCCA GTCGCTGGCC CACTGGGGAC TGGCTCCCAT GCGCCTGCTG 
TCCACGCTGG AGGCGTGGCT GCCCGCCAAG GAGCAGTTGC ACAGTTCGCC GAAGGTGGTG 
AGCTCGGCGC AGCAGCAGAA TGGCATCCAT TGGTATGCCA ATGCGCTCTA CAAGGTCAAG 
GACTACGTTC TGCCGCAGAG CTGGCGCCAC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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