Sap18 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sap18
Entrez Gene ID	20220
Full Name	Sin3-associated polypeptide 18
Synonyms	C530046K05Rik,D11Ertd539e,EMegR4,Sap18a,Sinbp1
General protein information	Preferred Names Sin3-associated polypeptide 18 Names histone deacetylase complex subunit SAP18 18 kDa Sin3-associated polypeptide sin3-associated polypeptide p18
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 30.51 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009119.3	NP_033145.2	histone deacetylase complex subunit SAP18

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	SAP18	NP_566050.1
Mus musculus (house mouse)	Gm10094	NP_001135913.1
Rattus norvegicus (Norway rat)	Sap18	NP_001028857.2
Gallus gallus (chicken)	SAP18	NP_989643.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003036	XP_311841.2
Homo sapiens (human)	SAP18	NP_005861.2
Pan troglodytes (chimpanzee)	SAP18	XP_509567.3
Mus musculus (house mouse)	Sap18	NP_033145.2
Drosophila melanogaster (fruit fly)	Bin1	NP_524377.1
Oryza sativa (rice)	Os02g0122000	NP_001045723.1
Canis lupus familiaris (dog)	SAP18	NP_001239090.1
Rattus norvegicus (Norway rat)	RGD1561590	XP_002727857.1
Danio rerio (zebrafish)	sap18	NP_957014.1
Xenopus tropicalis (tropical clawed frog)	sap18	NP_001016166.1
Macaca mulatta (Rhesus monkey)	SAP18	XP_001085342.2
Bos taurus (cattle)	SAP18	NP_001030544.2
Caenorhabditis elegans (roundworm)	CELE_C16C10.4	NP_497833.1

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins.
Paces-Fessy M, Boucher D, Petit E, Paute-Briand S, Blanchet-Tournier MF
The Biochemical journal378(Pt 2)353-62(2004 Mar)

Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex.
Cheng SY, Bishop JM
Proceedings of the National Academy of Sciences of the United States of America99(8)5442-7(2002 Apr)

The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA.
Wada M, Miyazawa H, Wang RS, Mizuno T, Sato A, Asashima M, Hanaoka F
Journal of biochemistry131(3)307-11(2002 Mar)

Molecular cloning and analysis of a group of genes differentially expressed in cells which overexpress the Hoxa-1 homeobox gene.
Shen J, Wu H, Gudas LJ
Experimental cell research259(1)274-83(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sap18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sap18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03550	NM_009119.3 Latest version!	Mus musculus Sin3-associated polypeptide 18 (Sap18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sap18 ( NM_009119.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033145.2 Mus musculus Sin3-associated polypeptide 18 (Sap18), mRNA.

Price & Availability↑

CloneID	OMu03550
Clone ID Related Accession (Same CDS sequence)	NM_009119.3
Accession Version	NM_009119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Mus musculus(house mouse)
Product	histone deacetylase complex subunit SAP18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF730347.1, AK146928.1, Z97062.1 and BM899282.1. On Dec 3, 2008 this sequence version replaced NM_009119.2. ##Evidence-Data-START## Transcript exon combination :: CF730347.1, Z97062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCGCCG CAGGGGTCGG AGGTCAGGGC GAGCGTCTCC CGGGCCGAAG GAGGAAGATG 
GCGGTGGAGT CGCGCGTTAC CCAGGAGGAA ATTAAGAAGG AGCCGGAGAA GCCGATCGAC 
CGCGAGAAGA CCTGCCCGCT CCTGCTGCGG GTCTTCACCA CCAACAACGG CCGCCACCAC 
CGAATGGACG AGTTCTCCCG CGGGAACGTG CCTTCGAGCG AGCTGCAGAT CTACACCTGG 
ATGGATGCAA CCTTGAAAGA ACTGACTAGT TTAGTGAAGG AAGTCTACCC AGAAGCCAGA 
AAGAAGGGCA CACACTTCAA TTTTGCAATT GTTTTTATGG ATCTTAAAAG ACCTGGATAT 
CGAGTTAAGG AGATTGGCAG CACCATGTCT GGCAGGAAGG GCACTGATGA CTCCATGACC 
CTGCAGTCAC AGAAGTTCCA AATAGGGGAT TATCTGGACA TAGCCATCAC GCCTCCAAAT 
CGGGCGCCGC CTTCGTCAGG GAGGATGAGA CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033145.2
CDS	38..556
Translation	MLAAGVGGQGERLPGRRRKMAVESRVTQEEIKKEPEKPIDREKTCPLLLRVFTTNNGRHHRMDEFSRGNVPSSELQIYTWMDATLKELTSLVKEVYPEARKKGTHFNFAIVFMDLKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPPNRAPPSSGRMRPY

Target ORF information:

RefSeq Version	NM_009119.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sin3-associated polypeptide 18 (Sap18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009119.3

ORF Insert Sequence:

ATGCTCGCCG CAGGGGTCGG AGGTCAGGGC GAGCGTCTCC CGGGCCGAAG GAGGAAGATG 
GCGGTGGAGT CGCGCGTTAC CCAGGAGGAA ATTAAGAAGG AGCCGGAGAA GCCGATCGAC 
CGCGAGAAGA CCTGCCCGCT CCTGCTGCGG GTCTTCACCA CCAACAACGG CCGCCACCAC 
CGAATGGACG AGTTCTCCCG CGGGAACGTG CCTTCGAGCG AGCTGCAGAT CTACACCTGG 
ATGGATGCAA CCTTGAAAGA ACTGACTAGT TTAGTGAAGG AAGTCTACCC AGAAGCCAGA 
AAGAAGGGCA CACACTTCAA TTTTGCAATT GTTTTTATGG ATCTTAAAAG ACCTGGATAT 
CGAGTTAAGG AGATTGGCAG CACCATGTCT GGCAGGAAGG GCACTGATGA CTCCATGACC 
CTGCAGTCAC AGAAGTTCCA AATAGGGGAT TATCTGGACA TAGCCATCAC GCCTCCAAAT 
CGGGCGCCGC CTTCGTCAGG GAGGATGAGA CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins.
The Biochemical journal378(Pt 2)353-62(2004 Mar)
Paces-Fessy M,Boucher D,Petit E,Paute-Briand S,Blanchet-Tournier MF

Suppressor of Fused represses Gli-mediated transcription by recruiting the SAP18-mSin3 corepressor complex.
Proceedings of the National Academy of Sciences of the United States of America99(8)5442-7(2002 Apr)
Cheng SY,Bishop JM

The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA.
Journal of biochemistry131(3)307-11(2002 Mar)
Wada M,Miyazawa H,Wang RS,Mizuno T,Sato A,Asashima M,Hanaoka F

Molecular cloning and analysis of a group of genes differentially expressed in cells which overexpress the Hoxa-1 homeobox gene.
Experimental cell research259(1)274-83(2000 Aug)
Shen J,Wu H,Gudas LJ