Rfc2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rfc2
Entrez Gene ID	19718
Full Name	replication factor C (activator 1) 2
Synonyms	2610008M13Rik,40kDa,AI326953,Recc2
General protein information	Preferred Names replication factor C (activator 1) 2 Names replication factor C subunit 2 A1 40 kDa subunit RF-C 40 kDa subunit RFC40 activator 1 40 kDa subunit activator 1 subunit 2 replication factor C 40 kDa subunit replication factor C, 40 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 G2\|5 74.68 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_020022.2	NP_064406.1	replication factor C subunit 2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rfc4	NP_593121.1
Homo sapiens (human)	RFC2	NP_852136.1
Macaca mulatta (Rhesus monkey)	RFC2	XP_001082511.1
Bos taurus (cattle)	RFC2	NP_001074372.1
Rattus norvegicus (Norway rat)	Rfc2	NP_446238.1
Caenorhabditis elegans (roundworm)	rfc-2	NP_500069.1
Saccharomyces cerevisiae (baker's yeast)	RFC4	NP_014547.1
Oryza sativa (rice)	Os04g0569000	NP_001053594.1
Xenopus tropicalis (tropical clawed frog)	rfc2	NP_001135488.1
Neurospora crassa	NCU10467	XP_001728317.1
Gallus gallus (chicken)	RFC2	NP_990861.1
Danio rerio (zebrafish)	rfc2	NP_001013344.2
Kluyveromyces lactis	KLLA0C03718g	XP_452362.1
Eremothecium gossypii	AGOS_ADR111W	NP_984207.1
Magnaporthe oryzae (rice blast fungus)	MGG_07526	XP_003711439.1
Mus musculus (house mouse)	Rfc2	NP_064406.1
Canis lupus familiaris (dog)	RFC2	XP_546916.3
Drosophila melanogaster (fruit fly)	RfC4	NP_523915.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007477	XP_308395.4
Pan troglodytes (chimpanzee)	RFC2	XP_001149874.1
Arabidopsis thaliana (thale cress)	RFC2	NP_176504.1

Gene Ontology
Bibliography

Related articles in PubMed

RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.
Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, Boulton SJ
Science (New York, N.Y.)342(6155)239-42(2013 Oct)

Generation and comparative analysis of approximately 3.3 Mb of mouse genomic sequence orthologous to the region of human chromosome 7q11.23 implicated in Williams syndrome.
DeSilva U, Elnitski L, Idol JR, Doyle JL, Gan W, Thomas JW, Schwartz S, Dietrich NL, Beckstrom-Sternberg SM, McDowell JC, Blakesley RW, Bouffard GG, Thomas PJ, Touchman JW, Miller W, Green ED
Genome research12(1)3-15(2002 Jan)

Integration of a c-myc transgene results in disruption of the mouse Gtf2ird1 gene, the homologue of the human GTF2IRD1 gene hemizygously deleted in Williams-Beuren syndrome.
Durkin ME, Keck-Waggoner CL, Popescu NC, Thorgeirsson SS
Genomics73(1)20-7(2001 Apr)

Fine-scale comparative mapping of the human 7q11.23 region and the orthologous region on mouse chromosome 5G: the low-copy repeats that flank the Williams-Beuren syndrome deletion arose at breakpoint sites of an evolutionary inversion(s).
Valero MC, de Luis O, Cruces J, Pérez Jurado LA
Genomics69(1)1-13(2000 Oct)

Divergent human and mouse orthologs of a novel gene (WBSCR15/Wbscr15) reside within the genomic interval commonly deleted in Williams syndrome.
Doyle JL, DeSilva U, Miller W, Green ED
Cytogenetics and cell genetics90(3-4)285-90(2000)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rfc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rfc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11689	NM_020022.2 Latest version!	Mus musculus replication factor C (activator 1) 2 (Rfc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rfc2 ( NM_020022.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064406.1 Mus musculus replication factor C (activator 1) 2 (Rfc2), mRNA.

Price & Availability↑

CloneID	OMu11689
Clone ID Related Accession (Same CDS sequence)	NM_020022.2
Accession Version	NM_020022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Mus musculus(house mouse)
Product	replication factor C subunit 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY338186.1 and AK075997.1. On or before Sep 3, 2004 this sequence version replaced XM_487905.1, XM_489822.1, NM_020022.1. ##Evidence-Data-START## Transcript exon combination :: BC099370.1, AK154681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTTC AGGAGAGTGG CTGTGACCCA TCCGAGAGTG GGGCGCAGGA GCCCTCGCCT 
GTCCCCAGCA AGACCGCCGG CCACTACGAG CTGCCGTGGG TTGAAAAATA CAGACCATTG 
AAGCTCAACG AAATAGTGGG CAATGAAGAC ACTGTGAGCA GGCTGGAGGT CTTTGCAAGA 
GAAGGCAATG TGCCCAATAT CATCATTGCT GGTCCCCCAG GAACTGGCAA GACAACCAGC 
ATCCTCTGCT TGGCTCGAGC CTTGCTGGGC CCCGCCCTGA AGGACGCAGT CCTGGAACTC 
AATGCCTCAA ATGACAGGGG CATCGATGTG GTGAGGAATA AAATCAAAAT GTTCGCCCAG 
CAGAAAGTCA CCCTTCCCAA GGGCCGGCAC AAGATCATCA TTCTAGATGA AGCAGACAGC 
ATGACGGACG GTGCCCAGCA GGCCCTCAGG AGAACCATGG AAATCTACTC GAAGACGACC 
CGATTCGCTT TGGCGTGTAA TGCTTCAGAC AAAATCATAG AGCCCATCCA GTCCCGCTGT 
GCGGTGCTCC GCTACACCAA GCTCACTGAC GCTCAGGTCC TCACCAGGCT CATGAATGTC 
ATCGAGAAGG AAAAGGTGCC ATACACAGAT GACGGCCTGG AAGCCATTAT CTTCACAGCC 
CAGGGAGACA TGCGCCAGGC ACTGAACAAC CTGCAGTCCA CCTTCTCAGG ATTTGGCTAT 
ATCAACAGTG AGAATGTATT CAAGGTCTGC GACGAGCCCC ACCCCCTGCT GGTGAAGGAG 
ATGATCCAGC ACTGCGTGGA CGCCAACATT GACGAGGCCT ATAAGATTCT TGCTCACCTG 
TGGCACCTGG GCTACTCACC AGAAGATGTC ATCGGCAACA TTTTCCGAGT CTGCAAAACC 
TTCCCGATGG CTGAATACTT GAAACTGGAG TTCATCAAGG AGATTGGATA CACTCACATG 
AAAGTGGCGG AAGGAGTGAA CTCCCTGCTG CAGATGGCTG GGCTCCTGGC CAGGCTGTGT 
CAGAAGACCA TGGCTCCAGT GGCCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064406.1
CDS	100..1149
Translation	MEVQESGCDPSESGAQEPSPVPSKTAGHYELPWVEKYRPLKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAVLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTRFALACNASDKIIEPIQSRCAVLRYTKLTDAQVLTRLMNVIEKEKVPYTDDGLEAIIFTAQGDMRQALNNLQSTFSGFGYINSENVFKVCDEPHPLLVKEMIQHCVDANIDEAYKILAHLWHLGYSPEDVIGNIFRVCKTFPMAEYLKLEFIKEIGYTHMKVAEGVNSLLQMAGLLARLCQKTMAPVAS

Target ORF information:

RefSeq Version	NM_020022.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus replication factor C (activator 1) 2 (Rfc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020022.2

ORF Insert Sequence:

ATGGAGGTTC AGGAGAGTGG CTGTGACCCA TCCGAGAGTG GGGCGCAGGA GCCCTCGCCT 
GTCCCCAGCA AGACCGCCGG CCACTACGAG CTGCCGTGGG TTGAAAAATA CAGACCATTG 
AAGCTCAACG AAATAGTGGG CAATGAAGAC ACTGTGAGCA GGCTGGAGGT CTTTGCAAGA 
GAAGGCAATG TGCCCAATAT CATCATTGCT GGTCCCCCAG GAACTGGCAA GACAACCAGC 
ATCCTCTGCT TGGCTCGAGC CTTGCTGGGC CCCGCCCTGA AGGACGCAGT CCTGGAACTC 
AATGCCTCAA ATGACAGGGG CATCGATGTG GTGAGGAATA AAATCAAAAT GTTCGCCCAG 
CAGAAAGTCA CCCTTCCCAA GGGCCGGCAC AAGATCATCA TTCTAGATGA AGCAGACAGC 
ATGACGGACG GTGCCCAGCA GGCCCTCAGG AGAACCATGG AAATCTACTC GAAGACGACC 
CGATTCGCTT TGGCGTGTAA TGCTTCAGAC AAAATCATAG AGCCCATCCA GTCCCGCTGT 
GCGGTGCTCC GCTACACCAA GCTCACTGAC GCTCAGGTCC TCACCAGGCT CATGAATGTC 
ATCGAGAAGG AAAAGGTGCC ATACACAGAT GACGGCCTGG AAGCCATTAT CTTCACAGCC 
CAGGGAGACA TGCGCCAGGC ACTGAACAAC CTGCAGTCCA CCTTCTCAGG ATTTGGCTAT 
ATCAACAGTG AGAATGTATT CAAGGTCTGC GACGAGCCCC ACCCCCTGCT GGTGAAGGAG 
ATGATCCAGC ACTGCGTGGA CGCCAACATT GACGAGGCCT ATAAGATTCT TGCTCACCTG 
TGGCACCTGG GCTACTCACC AGAAGATGTC ATCGGCAACA TTTTCCGAGT CTGCAAAACC 
TTCCCGATGG CTGAATACTT GAAACTGGAG TTCATCAAGG AGATTGGATA CACTCACATG 
AAAGTGGCGG AAGGAGTGAA CTCCCTGCTG CAGATGGCTG GGCTCCTGGC CAGGCTGTGT 
CAGAAGACCA TGGCTCCAGT GGCCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.
Science (New York, N.Y.)342(6155)239-42(2013 Oct)
Vannier JB,Sandhu S,Petalcorin MI,Wu X,Nabi Z,Ding H,Boulton SJ

Generation and comparative analysis of approximately 3.3 Mb of mouse genomic sequence orthologous to the region of human chromosome 7q11.23 implicated in Williams syndrome.
Genome research12(1)3-15(2002 Jan)
DeSilva U,Elnitski L,Idol JR,Doyle JL,Gan W,Thomas JW,Schwartz S,Dietrich NL,Beckstrom-Sternberg SM,McDowell JC,Blakesley RW,Bouffard GG,Thomas PJ,Touchman JW,Miller W,Green ED

Integration of a c-myc transgene results in disruption of the mouse Gtf2ird1 gene, the homologue of the human GTF2IRD1 gene hemizygously deleted in Williams-Beuren syndrome.
Genomics73(1)20-7(2001 Apr)
Durkin ME,Keck-Waggoner CL,Popescu NC,Thorgeirsson SS

Fine-scale comparative mapping of the human 7q11.23 region and the orthologous region on mouse chromosome 5G: the low-copy repeats that flank the Williams-Beuren syndrome deletion arose at breakpoint sites of an evolutionary inversion(s).
Genomics69(1)1-13(2000 Oct)
Valero MC,de Luis O,Cruces J,Pérez Jurado LA

Divergent human and mouse orthologs of a novel gene (WBSCR15/Wbscr15) reside within the genomic interval commonly deleted in Williams syndrome.
Cytogenetics and cell genetics90(3-4)285-90(2000)
Doyle JL,DeSilva U,Miller W,Green ED

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Rfc2 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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