Piga cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Piga
Entrez Gene ID	18700
Full Name	phosphatidylinositol glycan anchor biosynthesis, class A
Synonyms	AI194334,Pig-a
General protein information	Preferred Names phosphatidylinositol glycan anchor biosynthesis, class A Names N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein glcNAc-PI synthesis protein phosphatidylinositol glycan, class A phosphatidylinositol-glycan biosynthesis class A protein phosphatidylinositolglycan class A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F5\|X 76.49 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318428.2	XP_017173917.1	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X1
XM_006528758.3	XP_006528821.1	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X3
NM_011081.2	NP_035211.2	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
XM_011247793.2	XP_011246095.1	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X2
NM_011081.3	NP_035211.2	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
XM_017318428.1	XP_017173917.1	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C09713g	XP_452631.1
Macaca mulatta (Rhesus monkey)	PIGA	XP_002806202.1
Mus musculus (house mouse)	Piga	NP_035211.2
Arabidopsis thaliana (thale cress)	SETH2	NP_566874.1
Eremothecium gossypii	AGOS_AAL108C	NP_982434.1
Rattus norvegicus (Norway rat)	Piga	NP_001102286.1
Gallus gallus (chicken)	PIGA	NP_001025979.1
Caenorhabditis elegans (roundworm)	piga-1	NP_495840.1
Magnaporthe oryzae (rice blast fungus)	MGG_03933	XP_003719931.1
Pan troglodytes (chimpanzee)	PIGA	XP_520945.2
Danio rerio (zebrafish)	piga	NP_001119918.1
Neurospora crassa	NCU09757	XP_958197.1
Schizosaccharomyces pombe (fission yeast)	gpi3	NP_595519.1
Canis lupus familiaris (dog)	PIGA	XP_548868.1
Saccharomyces cerevisiae (baker's yeast)	SPT14	NP_015150.2
Homo sapiens (human)	PIGA	NP_002632.1
Drosophila melanogaster (fruit fly)	CG6401	NP_611257.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011274	XP_309373.3
Bos taurus (cattle)	PIGA	XP_002700441.2
Xenopus tropicalis (tropical clawed frog)	piga	XP_004911725.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycosylphosphatidylinositol biosynthesis and remodeling are required for neural tube closure, heart development, and cranial neural crest cell survival.
Lukacs M, Roberts T, Chatuverdi P, Stottmann RW
eLife8(2019 06)

Establishing a novel Pig-a gene mutation assay in L5178YTk
Wang Y, Revollo J, McKinzie P, Pearce MG, Dad A, Yucesoy B, Rosenfeldt H, Heflich RH, Dobrovolsky VN
Environmental and molecular mutagenesis59(1)4-17(2018 01)

Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tk
Bemis JC, Avlasevich SL, Labash C, McKinzie P, Revollo J, Dobrovolsky VN, Dertinger SD
Environmental and molecular mutagenesis59(1)18-29(2018 01)

Spectrum of benzo[a]pyrene-induced mutations in the Pig-a gene of L5178YTk
Revollo J, Wang Y, McKinzie P, Dad A, Pearce M, Heflich RH, Dobrovolsky VN
Mutation research8241-8(2017 12)

Single cell gel electrophoresis (SCGE) and Pig-a mutation assay in vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo[a]pyrene in Ogg1(-/-) mice.
Graupner A, Instanes C, Dertinger SD, Andersen JM, Lindeman B, Rongved TD, Brunborg G, Olsen AK
Mutation research. Genetic toxicology and environmental mutagenesis77234-41(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Piga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07435	NM_011081.3 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu07435	XM_017318428.2 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu56610	XM_006528758.3 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu68433	XM_011247793.2 Latest version!	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu07435	NM_011081.2	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu07435	XM_017318428.1	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Piga ( NM_011081.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035211.2 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), mRNA.

Price & Availability↑

CloneID	OMu07435
Clone ID Related Accession (Same CDS sequence)	XM_017318428.2 , NM_011081.2 , NM_011081.3 , XM_017318428.1
Accession Version	NM_011081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1493308800000
Organism	Mus musculus(House mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039802.1, BY270710.1, AK032283.1, BB707289.1 and BB771632.1. On Feb 27, 2007 this sequence version replaced NM_011081.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D26047.1, SRR1660815.21053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035211.2
CDS	117..1574
Translation	MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	NM_011081.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011081.2

ORF Insert Sequence:

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piga ( NM_011081.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035211.2 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07435
Clone ID Related Accession (Same CDS sequence)	XM_017318428.2 , NM_011081.2 , NM_011081.3 , XM_017318428.1
Accession Version	NM_011081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Mus musculus(house mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732475.6 and AK032283.1. On Nov 22, 2017 this sequence version replaced NM_011081.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D26047.1, SRR1660815.21053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035211.2
CDS	124..1581
Translation	MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	NM_011081.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011081.3

ORF Insert Sequence:

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piga ( XM_017318428.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173917.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07435
Clone ID Related Accession (Same CDS sequence)	XM_017318428.2 , NM_011081.2 , NM_011081.3 , XM_017318428.1
Accession Version	XM_017318428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173917.1
CDS	130..1587
Translation	MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	XM_017318428.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318428.2

ORF Insert Sequence:

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piga ( XM_017318428.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173917.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.

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CloneID	OMu07435
Clone ID Related Accession (Same CDS sequence)	XM_017318428.2 , NM_011081.2 , NM_011081.3 , XM_017318428.1
Accession Version	XM_017318428.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173917.1
CDS	129..1586
Translation	MANRRGGGQGQPPSVSPSPGSSGNLSDDRTCTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVITVTHAYGNRKGVRYLTNGLKVYYLPLRVMYNQSTATTLFHSLPLLRYIFVRERITIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSVITVVVVSRLVYRKGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	XM_017318428.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318428.1

ORF Insert Sequence:

ATGGCCAATA GAAGAGGAGG TGGGCAAGGC CAGCCTCCTT CAGTGTCGCC TTCCCCGGGT 
AGCTCTGGAA ACCTTTCCGA CGATAGGACC TGCACCCATA ATATATGCAT GGTGTCAGAC 
TTTTTCTACC CAAACATGGG AGGCGTGGAA AGCCACATTT ACCAGCTCTC TCAGTGCCTC 
ATTGAGAGAG GGCACAAGGT CATAACTGTC ACCCATGCTT ATGGAAATCG AAAGGGCGTC 
CGTTACCTCA CCAATGGCCT CAAAGTTTAC TATTTGCCTC TGAGAGTCAT GTACAACCAA 
TCTACAGCCA CGACTCTCTT TCACAGTCTG CCATTGCTCA GGTACATATT TGTTCGGGAG 
AGAATTACGA TAATCCATTC CCACAGTTCT TTCTCTGCCA TGGCCCATGA TGCTCTCTTC 
CACGCCAAGA CAATGGGGCT TCAGACAGTC TTCACAGACC ATTCCCTTTT CGGATTTGCT 
GATGTCAGCT CGGTGCTTAC AAACAAGCTT CTAACTGTGT CTCTTTGTGA CACAAACCAC 
ATCATTTGTG TCTCTTACAC TAGTAAGGAG AACACTGTAC TCAGAGCAGC ACTGAATCCT 
GAAATAGTGT CCGTCATTCC TAACGCTGTA GATCCTACTG ACTTCACTCC AGACCCATTT 
AGGAGGCATG ATAGTGTAAT AACTGTTGTT GTTGTCAGCA GACTTGTTTA CAGAAAAGGG 
ACTGATTTGC TTAGTGGTAT AATACCTGAA CTCTGTCAGA AATACCAAGA ATTACATTTC 
CTAATTGGAG GAGAGGGACC AAAGAGAATC ATTTTGGAAG AAGTACGGGA AAGATACCAA 
CTACATGACA GGGTGCAGCT TTTGGGAGCT TTAGAACACA AGGATGTTAG AAATGTCTTA 
GTTCAAGGAC ACATTTTTCT TAACACCTCC CTCACTGAAG CGTTCTGCAT GGCCATCGTG 
GAAGCTGCCA GTTGTGGTTT GCAGGTCGTC AGCACAAAGG TTGGTGGGAT TCCCGAAGTC 
CTTCCGGAAA GTCTTATTAT TCTCTGCGAG CCTTCAGTAA AATCTTTGTG TGACGGTCTG 
GAAAAAGCCA TTTTCCAAGT GAAGTCGGGG ACATTGCCAG CTCCAGAAAA TATCCACAAT 
GTAGTGAAGA CTTTCTACAC CTGGCGGAAC GTGGCAGAGA GAACTGAAAA GGTGTATGAG 
CGAGTGTCGA AGGAAACTGT TTTGCCAATG CACAAGAGAT TGGACAGGCT CATCTCACAC 
TGTGGCCCTG TGACGGGCTA CATGTTTGCT TTGCTGGCTG TGCTCAGCTA TCTCTTCCTC 
ATTTTCCTGC AGTGGATGAC TCCAGACTCT TTCATTGATG TTGCAATAGA TGCTACTGGG 
CCAAGGAGAG CCTGGACTCA TCAATGGCCT CGTGATAAGA AAAGAGATGA GAATGATAAG 
ATATCCCAAA GCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piga ( XM_006528758.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528821.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X3, mRNA.

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CloneID	OMu56610
Clone ID Related Accession (Same CDS sequence)	XM_006528758.3
Accession Version	XM_006528758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCG TGGAAGCTGC CAGTTGTGGT TTGCAGGTCG TCAGCACAAA GGTTGGTGGG 
ATTCCCGAAG TCCTTCCGGA AAGTCTTATT ATTCTCTGCG AGCCTTCAGT AAAATCTTTG 
TGTGACGGTC TGGAAAAAGC CATTTTCCAA GTGAAGTCGG GGACATTGCC AGCTCCAGAA 
AATATCCACA ATGTAGTGAA GACTTTCTAC ACCTGGCGGA ACGTGGCAGA GAGAACTGAA 
AAGGTGTATG AGCGAGTGTC GAAGGAAACT GTTTTGCCAA TGCACAAGAG ATTGGACAGG 
CTCATCTCAC ACTGTGGCCC TGTGACGGGC TACATGTTTG CTTTGCTGGC TGTGCTCAGC 
TATCTCTTCC TCATTTTCCT GCAGTGGATG ACTCCAGACT CTTTCATTGA TGTTGCAATA 
GATGCTACTG GGCCAAGGAG AGCCTGGACT CATCAATGGC CTCGTGATAA GAAAAGAGAT 
GAGAATGATA AGATATCCCA AAGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528821.1
CDS	790..1299
Translation	MAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	XM_006528758.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528758.3

ORF Insert Sequence:

ATGGCCATCG TGGAAGCTGC CAGTTGTGGT TTGCAGGTCG TCAGCACAAA GGTTGGTGGG 
ATTCCCGAAG TCCTTCCGGA AAGTCTTATT ATTCTCTGCG AGCCTTCAGT AAAATCTTTG 
TGTGACGGTC TGGAAAAAGC CATTTTCCAA GTGAAGTCGG GGACATTGCC AGCTCCAGAA 
AATATCCACA ATGTAGTGAA GACTTTCTAC ACCTGGCGGA ACGTGGCAGA GAGAACTGAA 
AAGGTGTATG AGCGAGTGTC GAAGGAAACT GTTTTGCCAA TGCACAAGAG ATTGGACAGG 
CTCATCTCAC ACTGTGGCCC TGTGACGGGC TACATGTTTG CTTTGCTGGC TGTGCTCAGC 
TATCTCTTCC TCATTTTCCT GCAGTGGATG ACTCCAGACT CTTTCATTGA TGTTGCAATA 
GATGCTACTG GGCCAAGGAG AGCCTGGACT CATCAATGGC CTCGTGATAA GAAAAGAGAT 
GAGAATGATA AGATATCCCA AAGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piga ( XM_011247793.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246095.1 Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68433
Clone ID Related Accession (Same CDS sequence)	XM_011247793.2
Accession Version	XM_011247793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACCTG CATGTAACAG ACAAAGAAAC AGTCTCAGAG AGGGGACTGA TTTGCTTAGT 
GGTATAATAC CTGAACTCTG TCAGAAATAC CAAGAATTAC ATTTCCTAAT TGGAGGAGAG 
GGACCAAAGA GAATCATTTT GGAAGAAGTA CGGGAAAGAT ACCAACTACA TGACAGGGTG 
CAGCTTTTGG GAGCTTTAGA ACACAAGGAT GTTAGAAATG TCTTAGTTCA AGGACACATT 
TTTCTTAACA CCTCCCTCAC TGAAGCGTTC TGCATGGCCA TCGTGGAAGC TGCCAGTTGT 
GGTTTGCAGG TCGTCAGCAC AAAGGTTGGT GGGATTCCCG AAGTCCTTCC GGAAAGTCTT 
ATTATTCTCT GCGAGCCTTC AGTAAAATCT TTGTGTGACG GTCTGGAAAA AGCCATTTTC 
CAAGTGAAGT CGGGGACATT GCCAGCTCCA GAAAATATCC ACAATGTAGT GAAGACTTTC 
TACACCTGGC GGAACGTGGC AGAGAGAACT GAAAAGGTGT ATGAGCGAGT GTCGAAGGAA 
ACTGTTTTGC CAATGCACAA GAGATTGGAC AGGCTCATCT CACACTGTGG CCCTGTGACG 
GGCTACATGT TTGCTTTGCT GGCTGTGCTC AGCTATCTCT TCCTCATTTT CCTGCAGTGG 
ATGACTCCAG ACTCTTTCAT TGATGTTGCA ATAGATGCTA CTGGGCCAAG GAGAGCCTGG 
ACTCATCAAT GGCCTCGTGA TAAGAAAAGA GATGAGAATG ATAAGATATC CCAAAGCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246095.1
CDS	506..1288
Translation	MLPACNRQRNSLREGTDLLSGIIPELCQKYQELHFLIGGEGPKRIILEEVRERYQLHDRVQLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTKVGGIPEVLPESLIILCEPSVKSLCDGLEKAIFQVKSGTLPAPENIHNVVKTFYTWRNVAERTEKVYERVSKETVLPMHKRLDRLISHCGPVTGYMFALLAVLSYLFLIFLQWMTPDSFIDVAIDATGPRRAWTHQWPRDKKRDENDKISQSR

Target ORF information:

RefSeq Version	XM_011247793.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol glycan anchor biosynthesis, class A (Piga), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247793.2

ORF Insert Sequence:

ATGTTACCTG CATGTAACAG ACAAAGAAAC AGTCTCAGAG AGGGGACTGA TTTGCTTAGT 
GGTATAATAC CTGAACTCTG TCAGAAATAC CAAGAATTAC ATTTCCTAAT TGGAGGAGAG 
GGACCAAAGA GAATCATTTT GGAAGAAGTA CGGGAAAGAT ACCAACTACA TGACAGGGTG 
CAGCTTTTGG GAGCTTTAGA ACACAAGGAT GTTAGAAATG TCTTAGTTCA AGGACACATT 
TTTCTTAACA CCTCCCTCAC TGAAGCGTTC TGCATGGCCA TCGTGGAAGC TGCCAGTTGT 
GGTTTGCAGG TCGTCAGCAC AAAGGTTGGT GGGATTCCCG AAGTCCTTCC GGAAAGTCTT 
ATTATTCTCT GCGAGCCTTC AGTAAAATCT TTGTGTGACG GTCTGGAAAA AGCCATTTTC 
CAAGTGAAGT CGGGGACATT GCCAGCTCCA GAAAATATCC ACAATGTAGT GAAGACTTTC 
TACACCTGGC GGAACGTGGC AGAGAGAACT GAAAAGGTGT ATGAGCGAGT GTCGAAGGAA 
ACTGTTTTGC CAATGCACAA GAGATTGGAC AGGCTCATCT CACACTGTGG CCCTGTGACG 
GGCTACATGT TTGCTTTGCT GGCTGTGCTC AGCTATCTCT TCCTCATTTT CCTGCAGTGG 
ATGACTCCAG ACTCTTTCAT TGATGTTGCA ATAGATGCTA CTGGGCCAAG GAGAGCCTGG 
ACTCATCAAT GGCCTCGTGA TAAGAAAAGA GATGAGAATG ATAAGATATC CCAAAGCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycosylphosphatidylinositol biosynthesis and remodeling are required for neural tube closure, heart development, and cranial neural crest cell survival.
eLife8(2019 06)
Lukacs M,Roberts T,Chatuverdi P,Stottmann RW

Establishing a novel Pig-a gene mutation assay in L5178YTk
Environmental and molecular mutagenesis59(1)4-17(2018 01)
Wang Y,Revollo J,McKinzie P,Pearce MG,Dad A,Yucesoy B,Rosenfeldt H,Heflich RH,Dobrovolsky VN

Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tk
Environmental and molecular mutagenesis59(1)18-29(2018 01)
Bemis JC,Avlasevich SL,Labash C,McKinzie P,Revollo J,Dobrovolsky VN,Dertinger SD

Spectrum of benzo[a]pyrene-induced mutations in the Pig-a gene of L5178YTk
Mutation research8241-8(2017 12)
Revollo J,Wang Y,McKinzie P,Dad A,Pearce M,Heflich RH,Dobrovolsky VN

Single cell gel electrophoresis (SCGE) and Pig-a mutation assay in vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo[a]pyrene in Ogg1(-/-) mice.
Mutation research. Genetic toxicology and environmental mutagenesis77234-41(2014 Sep)
Graupner A,Instanes C,Dertinger SD,Andersen JM,Lindeman B,Rongved TD,Brunborg G,Olsen AK

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Piga cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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