Il12b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il12b
Entrez Gene ID	16160
Full Name	interleukin 12b
Synonyms	Il-12b,Il-12p40,Il12p40,p40
General protein information	Preferred Names interleukin 12b Names interleukin-12 subunit beta CLMF p40 IL-12 p40 subunit IL-12 subunit p40 IL-23 subunit p40 cytotoxic lymphocyte maturation factor 40 kDa subunit interleukin-12 p40 subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B1.1\|11 25.94 cM
Summary	This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001303244.1	NP_001290173.1	interleukin-12 subunit beta precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	IL12B	NP_001038190.1
Bos taurus (cattle)	IL12B	NP_776781.1
Homo sapiens (human)	IL12B	NP_002178.2
Gallus gallus (chicken)	IL12B	NP_998736.1
Canis lupus familiaris (dog)	IL12B	NP_001003292.1
Mus musculus (house mouse)	Il12b	NP_032378.1
Danio rerio (zebrafish)	il12ba	NP_001007109.1
Pan troglodytes (chimpanzee)	IL12B	XP_527101.2
Rattus norvegicus (Norway rat)	Il12b	NP_072133.1
Danio rerio (zebrafish)	il12bb	XP_002666092.2

Gene Ontology
Bibliography

Related articles in PubMed

Temporally Distinct Functions of the Cytokines IL-12 and IL-23 Drive Chronic Colon Inflammation in Response to Intestinal Barrier Impairment.
Eftychi C, Schwarzer R, Vlantis K, Wachsmuth L, Basic M, Wagle P, Neurath MF, Becker C, Bleich A, Pasparakis M
Immunity51(2)367-380.e4(2019 08)

Deficiency of IL12p40 (Interleukin 12 p40) Promotes Ang II (Angiotensin II)-Induced Abdominal Aortic Aneurysm.
Sharma N, Dev R, Belenchia AM, Aroor AR, Whaley-Connell A, Pulakat L, Hans CP
Arteriosclerosis, thrombosis, and vascular biology39(2)212-223(2019 02)

Neutrophil Cathepsin G Regulates Dendritic Cell Production of IL-12 during Development of CD4 T Cell Responses to Antigens in the Skin.
Kish DD, Min S, Dvorina N, Baldwin WM 3rd, Stohlman SA, Fairchild RL
Journal of immunology (Baltimore, Md. : 1950)202(4)1045-1056(2019 02)

CNBP controls IL-12 gene transcription and Th1 immunity.
Chen Y, Sharma S, Assis PA, Jiang Z, Elling R, Olive AJ, Hang S, Bernier J, Huh JR, Sassetti CM, Knipe DM, Gazzinelli RT, Fitzgerald KA
The Journal of experimental medicine215(12)3136-3150(2018 12)

DAMP-Inducing Adjuvant and PAMP Adjuvants Parallelly Enhance Protective Type-2 and Type-1 Immune Responses to Influenza Split Vaccination.
Hayashi T, Momota M, Kuroda E, Kusakabe T, Kobari S, Makisaka K, Ohno Y, Suzuki Y, Nakagawa F, Lee MSJ, Coban C, Onodera R, Higashi T, Motoyama K, Ishii KJ, Arima H
Frontiers in immunology92619(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Il12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu67996	NM_001303244.1 Latest version!	Mus musculus interleukin 12b (Il12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il12b ( NM_001303244.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001290173.1 Mus musculus interleukin 12b (Il12b), mRNA.

Price & Availability↑

CloneID	OMu67996
Clone ID Related Accession (Same CDS sequence)	NM_001303244.1
Accession Version	NM_001303244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	interleukin-12 subunit beta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY154173.1, M86671.1 and AL669944.8. On Dec 23, 2014 this sequence version replaced XM_006532306.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M86671.1, AK162981.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTCCTC AGAAGCTAAC CATCTCCTGG TTTGCCATCG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGACGTT TATGTTGTAG AGGTGGACTG GACTCCCGAT 
GCCCCTGGAG AAACAGTGAA CCTCACCTGT GACACGCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACATGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAAA 
GAGTTTCTAG ATGCTGGCCA GTACACCTGC CACAAAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CTGAAATTTT AAAAAATTTC 
AAAAACAAGA CTTTCCTGAA GTGTGAAGCA CCAAATTACT CCGGACGGTT CACGTGCTCA 
TGGCTGGTGC AAAGAAACAT GGACTTGAAG TTCAACATCA AGAGCAGTAG CAGTTCCCCT 
GACTCTCGGG CAGTGACATG TGGAATGGCG TCTCTGTCTG CAGAGAAGGT CACACTGGAC 
CAAAGGGACT ATGAGAAGTA TTCAGTGTCC TGCCAGGAGG ATGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGCGTTG GAAGCACGGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCAG ACCCGCCCAA GAACTTGCAG 
ATGAAGCCTT TGAAGAACTC ACAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTTGTTCGAA TCCAGCGCAA GAAAGAAAAG 
ATGAAGGAGA CAGAGGAGGG GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACATCT 
ACCGAAGTCC AATGCAAAGG CGGGAATGTC TGCGTGCAAG CTCAGGATCG CTATTACAAT 
TCCTCATGCA GCAAGTGGGC ATGTGTTCCC TGCAGGGTCC GATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290173.1
CDS	57..1064
Translation	MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Target ORF information:

RefSeq Version	NM_001303244.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus interleukin 12b (Il12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303244.1

ORF Insert Sequence:

ATGTGTCCTC AGAAGCTAAC CATCTCCTGG TTTGCCATCG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGACGTT TATGTTGTAG AGGTGGACTG GACTCCCGAT 
GCCCCTGGAG AAACAGTGAA CCTCACCTGT GACACGCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACATGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAAA 
GAGTTTCTAG ATGCTGGCCA GTACACCTGC CACAAAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CTGAAATTTT AAAAAATTTC 
AAAAACAAGA CTTTCCTGAA GTGTGAAGCA CCAAATTACT CCGGACGGTT CACGTGCTCA 
TGGCTGGTGC AAAGAAACAT GGACTTGAAG TTCAACATCA AGAGCAGTAG CAGTTCCCCT 
GACTCTCGGG CAGTGACATG TGGAATGGCG TCTCTGTCTG CAGAGAAGGT CACACTGGAC 
CAAAGGGACT ATGAGAAGTA TTCAGTGTCC TGCCAGGAGG ATGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGCGTTG GAAGCACGGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCAG ACCCGCCCAA GAACTTGCAG 
ATGAAGCCTT TGAAGAACTC ACAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTTGTTCGAA TCCAGCGCAA GAAAGAAAAG 
ATGAAGGAGA CAGAGGAGGG GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACATCT 
ACCGAAGTCC AATGCAAAGG CGGGAATGTC TGCGTGCAAG CTCAGGATCG CTATTACAAT 
TCCTCATGCA GCAAGTGGGC ATGTGTTCCC TGCAGGGTCC GATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporally Distinct Functions of the Cytokines IL-12 and IL-23 Drive Chronic Colon Inflammation in Response to Intestinal Barrier Impairment.
Immunity51(2)367-380.e4(2019 08)
Eftychi C,Schwarzer R,Vlantis K,Wachsmuth L,Basic M,Wagle P,Neurath MF,Becker C,Bleich A,Pasparakis M

Deficiency of IL12p40 (Interleukin 12 p40) Promotes Ang II (Angiotensin II)-Induced Abdominal Aortic Aneurysm.
Arteriosclerosis, thrombosis, and vascular biology39(2)212-223(2019 02)
Sharma N,Dev R,Belenchia AM,Aroor AR,Whaley-Connell A,Pulakat L,Hans CP

Neutrophil Cathepsin G Regulates Dendritic Cell Production of IL-12 during Development of CD4 T Cell Responses to Antigens in the Skin.
Journal of immunology (Baltimore, Md. : 1950)202(4)1045-1056(2019 02)
Kish DD,Min S,Dvorina N,Baldwin WM 3rd,Stohlman SA,Fairchild RL

CNBP controls IL-12 gene transcription and Th1 immunity.
The Journal of experimental medicine215(12)3136-3150(2018 12)
Chen Y,Sharma S,Assis PA,Jiang Z,Elling R,Olive AJ,Hang S,Bernier J,Huh JR,Sassetti CM,Knipe DM,Gazzinelli RT,Fitzgerald KA

DAMP-Inducing Adjuvant and PAMP Adjuvants Parallelly Enhance Protective Type-2 and Type-1 Immune Responses to Influenza Split Vaccination.
Frontiers in immunology92619(2018)
Hayashi T,Momota M,Kuroda E,Kusakabe T,Kobari S,Makisaka K,Ohno Y,Suzuki Y,Nakagawa F,Lee MSJ,Coban C,Onodera R,Higashi T,Motoyama K,Ishii KJ,Arima H

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Il12b cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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