Gspt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gspt1
Entrez Gene ID	14852
Full Name	G1 to S phase transition 1
Synonyms	AI314175,AI326449,AV307676,C79774,G1st,Gst-1
General protein information	Preferred Names G1 to S phase transition 1 Names eukaryotic peptide chain release factor GTP-binding subunit ERF3A G1 to S phase transition protein 1 homolog G1 to phase transition 1 G1-to-S transition GTP binding protein eRF3a eukaryotic peptide chain release factor subunit 3a eukaryotic polypeptide chain release factor 3 guanine nucleotide regulatory protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 6.38 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130008.1	NP_001123480.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
NM_146066.2	NP_666178.2	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GSPT1	XP_001140542.3
Rattus norvegicus (Norway rat)	Gspt1	NP_001003978.1
Gallus gallus (chicken)	GSPT1	NP_001129149.1
Saccharomyces cerevisiae (baker's yeast)	SUP35	NP_010457.3
Kluyveromyces lactis	KLLA0D17424g	XP_453831.1
Oryza sativa (rice)	Os04g0270100	NP_001052341.1
Homo sapiens (human)	GSPT1	NP_001123478.1
Danio rerio (zebrafish)	gspt1	NP_001003992.1
Eremothecium gossypii	AGOS_AGL145W	NP_986522.2
Macaca mulatta (Rhesus monkey)	LOC716860	XP_001107851.2
Bos taurus (cattle)	GSPT1	NP_001091540.2
Mus musculus (house mouse)	Gspt1	NP_001123480.1
Rattus norvegicus (Norway rat)	LOC100911685	XP_006245802.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009310	XP_320105.3
Neurospora crassa	NCU04790	XP_960416.1
Schizosaccharomyces pombe (fission yeast)	sup35	NP_588225.1
Danio rerio (zebrafish)	gspt1l	NP_942101.1
Arabidopsis thaliana (thale cress)	AT1G18070	NP_001185026.1
Magnaporthe oryzae (rice blast fungus)	MGG_00449	XP_003718618.1
Canis lupus familiaris (dog)	GSPT1	XP_005621589.1
Drosophila melanogaster (fruit fly)	Elf	NP_001260415.1
Xenopus tropicalis (tropical clawed frog)	gspt1	NP_001015805.2

Gene Ontology
Bibliography

Related articles in PubMed

A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury.
Ishii T, Ueyama T, Shigyo M, Kohta M, Kondoh T, Kuboyama T, Uebi T, Hamada T, Gutmann DH, Aiba A, Kohmura E, Tohda C, Saito N
The Journal of biological chemistry292(4)1240-1250(2017 01)

Functional role of Tia1/Pub1 and Sup35 prion domains: directing protein synthesis machinery to the tubulin cytoskeleton.
Li X, Rayman JB, Kandel ER, Derkatch IL
Molecular cell55(2)305-18(2014 Jul)

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
Figaro S, Scrima N, Buckingham RH, Heurgué-Hamard V
FEBS letters582(16)2352-6(2008 Jul)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Molecular cloning of a novel member of the eukaryotic polypeptide chain-releasing factors (eRF). Its identification as eRF3 interacting with eRF1.
Hoshino S, Imai M, Mizutani M, Kikuchi Y, Hanaoka F, Ui M, Katada T
The Journal of biological chemistry273(35)22254-9(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gspt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gspt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21502	NM_146066.2 Latest version!	Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu21503	NM_001130008.1 Latest version!	Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gspt1 ( NM_146066.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666178.2 Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21502
Clone ID Related Accession (Same CDS sequence)	NM_146066.2
Accession Version	NM_146066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB631229.1, AK148603.1, AC087541.23 and CJ260597.1. On Jul 10, 2008 this sequence version replaced NM_146066.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK148603.1, AK030760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCTA GCAGCGGCGG TGGCGGCGGT GGCGGCGGCG GCGGGAGCAG CAGCAGCAGC 
GACTCTGCGC CCGACTGCTG GGACCAGACG GATATGGAGG CCCCCGGGCC CGGGCCTTGC 
GGCGGCGGCG GGAGCGGCAG CGGCTCCATG GCCGCGGTGG CCGAGGCCCA GCGTGAGAAC 
CTCAGCGCGG CTTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCTTTCGT CCCCAACGTC 
CACGCCGCTG AATTCGTGCC GTCCTTCCTG CGGGGTCCGG CCCAGCCGCC GCTATCCCCA 
GCTGGCGCAG CCGGAGGCGA CCACGGAGCA GGCAGCGGCG CGGGAGGCCC TTCGGAACCT 
GTAGAGTCCT CTCAAGACCA GTCGTGTGAA GGTTCAAATT CTACTGTTAG CATGGAACTT 
TCAGAACCTG TTGTAGAAAA TGGAGAGACA GAAATGTCCC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC GGAGCCAGGG GGTGGTTCCT CAGGAGATGG AAGGCCACCA 
GAGGAAAGCA CCCAAGAAAT GATGGAGGAG GAGGAAGAAA TACCGAAACC TAAGTCAGCG 
GTGGCACCAC CAGGTGCTCC CAAGAAGGAA CATGTAAATG TAGTGTTCAT CGGACATGTT 
GATGCAGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTCGACAAA 
AGGACACTTG AGAAGTATGA ACGAGAAGCT AAAGAAAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACGAA TCAGGAAGAA CGAGACAAGG GCAAAACCGT GGAGGTGGGT 
CGTGCCTATT TTGAAACGGA AAAGAAGCAT TTTACAATTC TAGATGCTCC TGGCCATAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ACTTGGCTGT ACTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTCAAA CACTTAATTG TGCTTATTAA TAAGATGGAT 
GATCCAACGG TAAACTGGAG CAACGAGAGA TATGAAGAAT GTAAAGAGAA ACTAGTACCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ATTTTATGCC GTGCTCAGGA 
CTGACAGGAG CGAATCTTAA AGAACAATCA GATTTCTGTC CTTGGTACAT TGGCTTACCA 
TTTATTCCAT ATCTGGATAA TTTGCCAAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TCGATAAGTA CAAGGATATG GGGACTGTGG TCCTGGGAAA GCTGGAATCT 
GGATCTATTT GTAAAGGCCA GCAGCTTGTA ATGATGCCAA ATAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCTGATGA TGTAGAAACG GACTCTGTAG CTCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAGGAAGAG ATTCTTCCAG GATTCATCCT TTGTGATCTT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCTCAGA TAGTGATTAT AGAGCACAAG 
TCCATCATCT GCCCAGGGTA TAATGCGGTG CTGCATATTC ATACCTGTAT CGAAGAAGTC 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAGAAATCAG GAGAGAAAAG TAAGACTCGA 
CCCCGTTTTG TAAAACAAGA TCAAGTATGC ATTGCGCGTT TAAGGACAGC AGGAACCATC 
TGCCTGGAGA CCTTTAAGGA CTTCCCTCAG ATGGGACGTT TTACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666178.2
CDS	329..2239
Translation	MDPSSGGGGGGGGGGSSSSSDSAPDCWDQTDMEAPGPGPCGGGGSGSGSMAAVAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAQPPLSPAGAAGGDHGAGSGAGGPSEPVESSQDQSCEGSNSTVSMELSEPVVENGETEMSPEESWEHKEEISEAEPGGGSSGDGRPPEESTQEMMEEEEEIPKPKSAVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGFILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_146066.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146066.2

ORF Insert Sequence:

ATGGATCCTA GCAGCGGCGG TGGCGGCGGT GGCGGCGGCG GCGGGAGCAG CAGCAGCAGC 
GACTCTGCGC CCGACTGCTG GGACCAGACG GATATGGAGG CCCCCGGGCC CGGGCCTTGC 
GGCGGCGGCG GGAGCGGCAG CGGCTCCATG GCCGCGGTGG CCGAGGCCCA GCGTGAGAAC 
CTCAGCGCGG CTTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCTTTCGT CCCCAACGTC 
CACGCCGCTG AATTCGTGCC GTCCTTCCTG CGGGGTCCGG CCCAGCCGCC GCTATCCCCA 
GCTGGCGCAG CCGGAGGCGA CCACGGAGCA GGCAGCGGCG CGGGAGGCCC TTCGGAACCT 
GTAGAGTCCT CTCAAGACCA GTCGTGTGAA GGTTCAAATT CTACTGTTAG CATGGAACTT 
TCAGAACCTG TTGTAGAAAA TGGAGAGACA GAAATGTCCC CAGAAGAATC ATGGGAGCAC 
AAAGAAGAAA TAAGTGAAGC GGAGCCAGGG GGTGGTTCCT CAGGAGATGG AAGGCCACCA 
GAGGAAAGCA CCCAAGAAAT GATGGAGGAG GAGGAAGAAA TACCGAAACC TAAGTCAGCG 
GTGGCACCAC CAGGTGCTCC CAAGAAGGAA CATGTAAATG TAGTGTTCAT CGGACATGTT 
GATGCAGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTCGACAAA 
AGGACACTTG AGAAGTATGA ACGAGAAGCT AAAGAAAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACGAA TCAGGAAGAA CGAGACAAGG GCAAAACCGT GGAGGTGGGT 
CGTGCCTATT TTGAAACGGA AAAGAAGCAT TTTACAATTC TAGATGCTCC TGGCCATAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ACTTGGCTGT ACTGGTAATC 
TCAGCCAGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCAATGTTGG CAAAGACAGC AGGTGTCAAA CACTTAATTG TGCTTATTAA TAAGATGGAT 
GATCCAACGG TAAACTGGAG CAACGAGAGA TATGAAGAAT GTAAAGAGAA ACTAGTACCA 
TTTTTGAAAA AAGTTGGCTT CAATCCCAAA AAGGACATTC ATTTTATGCC GTGCTCAGGA 
CTGACAGGAG CGAATCTTAA AGAACAATCA GATTTCTGTC CTTGGTACAT TGGCTTACCA 
TTTATTCCAT ATCTGGATAA TTTGCCAAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TCGATAAGTA CAAGGATATG GGGACTGTGG TCCTGGGAAA GCTGGAATCT 
GGATCTATTT GTAAAGGCCA GCAGCTTGTA ATGATGCCAA ATAAGCACAA CGTGGAAGTT 
CTTGGAATAC TTTCTGATGA TGTAGAAACG GACTCTGTAG CTCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGAATTGA AGAGGAAGAG ATTCTTCCAG GATTCATCCT TTGTGATCTT 
AATAATCTTT GTCATTCTGG ACGCACATTT GATGCTCAGA TAGTGATTAT AGAGCACAAG 
TCCATCATCT GCCCAGGGTA TAATGCGGTG CTGCATATTC ATACCTGTAT CGAAGAAGTC 
GAAATAACAG CCTTAATCTG CTTGGTAGAC AAGAAATCAG GAGAGAAAAG TAAGACTCGA 
CCCCGTTTTG TAAAACAAGA TCAAGTATGC ATTGCGCGTT TAAGGACAGC AGGAACCATC 
TGCCTGGAGA CCTTTAAGGA CTTCCCTCAG ATGGGACGTT TTACCTTAAG AGATGAGGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gspt1 ( NM_001130008.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001123480.1 Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu21503
Clone ID Related Accession (Same CDS sequence)	NM_001130008.1
Accession Version	NM_001130008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB631229.1, AK148603.1, AK032059.1, AC087541.23 and CJ260597.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in an internal exon, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK032059.1, AB003502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCTA GCAGCGGCGG TGGCGGCGGT GGCGGCGGCG GCGGGAGCAG CAGCAGCAGC 
GACTCTGCGC CCGACTGCTG GGACCAGACG GATATGGAGG CCCCCGGGCC CGGGCCTTGC 
GGCGGCGGCG GGAGCGGCAG CGGCTCCATG GCCGCGGTGG CCGAGGCCCA GCGTGAGAAC 
CTCAGCGCGG CTTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCTTTCGT CCCCAACGTC 
CACGCCGCTG AATTCGTGCC GTCCTTCCTG CGGGGTCCGG CCCAGCCGCC GCTATCCCCA 
GCTGGCGCAG CCGGAGGCGA CCACGGAGCA GGCAGCGGCG CGGGAGGCCC TTCGGAACCT 
GTAGAGTCCT CTCAAGACCA GTCGTGTGAA GGTTCAAATT CTACTGTTAG CATGGAACTT 
TCAGAACCTG TTGAAAATGG AGAGACAGAA ATGTCCCCAG AAGAATCATG GGAGCACAAA 
GAAGAAATAA GTGAAGCGGA GCCAGGGGGT GGTTCCTCAG GAGATGGAAG GCCACCAGAG 
GAAAGCACCC AAGAAATGAT GGAGGAGGAG GAAGAAATAC CGAAACCTAA GTCAGCGGTG 
GCACCACCAG GTGCTCCCAA GAAGGAACAT GTAAATGTAG TGTTCATCGG ACATGTTGAT 
GCAGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT CGACAAAAGG 
ACACTTGAGA AGTATGAACG AGAAGCTAAA GAAAAAAACA GAGAAACTTG GTACTTGTCT 
TGGGCCTTAG ACACGAATCA GGAAGAACGA GACAAGGGCA AAACCGTGGA GGTGGGTCGT 
GCCTATTTTG AAACGGAAAA GAAGCATTTT ACAATTCTAG ATGCTCCTGG CCATAAGAGT 
TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGACT TGGCTGTACT GGTAATCTCA 
GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG AGAACATGCA 
ATGTTGGCAA AGACAGCAGG TGTCAAACAC TTAATTGTGC TTATTAATAA GATGGATGAT 
CCAACGGTAA ACTGGAGCAA CGAGAGATAT GAAGAATGTA AAGAGAAACT AGTACCATTT 
TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCATT TTATGCCGTG CTCAGGACTG 
ACAGGAGCGA ATCTTAAAGA ACAATCAGAT TTCTGTCCTT GGTACATTGG CTTACCATTT 
ATTCCATATC TGGATAATTT GCCAAACTTC AATAGATCAG TTGATGGACC AATCAGGCTG 
CCAATTGTCG ATAAGTACAA GGATATGGGG ACTGTGGTCC TGGGAAAGCT GGAATCTGGA 
TCTATTTGTA AAGGCCAGCA GCTTGTAATG ATGCCAAATA AGCACAACGT GGAAGTTCTT 
GGAATACTTT CTGATGATGT AGAAACGGAC TCTGTAGCTC CAGGTGAAAA CCTCAAAATC 
AGACTGAAAG GAATTGAAGA GGAAGAGATT CTTCCAGGAT TCATCCTTTG TGATCTTAAT 
AATCTTTGTC ATTCTGGACG CACATTTGAT GCTCAGATAG TGATTATAGA GCACAAGTCC 
ATCATCTGCC CAGGGTATAA TGCGGTGCTG CATATTCATA CCTGTATCGA AGAAGTCGAA 
ATAACAGCCT TAATCTGCTT GGTAGACAAG AAATCAGGAG AGAAAAGTAA GACTCGACCC 
CGTTTTGTAA AACAAGATCA AGTATGCATT GCGCGTTTAA GGACAGCAGG AACCATCTGC 
CTGGAGACCT TTAAGGACTT CCCTCAGATG GGACGTTTTA CCTTAAGAGA TGAGGGTAAG 
ACCATTGCAA TTGGAAAAGT TCTGAAACTG GTTCCAGAGA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123480.1
CDS	329..2236
Translation	MDPSSGGGGGGGGGGSSSSSDSAPDCWDQTDMEAPGPGPCGGGGSGSGSMAAVAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAQPPLSPAGAAGGDHGAGSGAGGPSEPVESSQDQSCEGSNSTVSMELSEPVENGETEMSPEESWEHKEEISEAEPGGGSSGDGRPPEESTQEMMEEEEEIPKPKSAVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGFILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001130008.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G1 to S phase transition 1 (Gspt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130008.1

ORF Insert Sequence:

ATGGATCCTA GCAGCGGCGG TGGCGGCGGT GGCGGCGGCG GCGGGAGCAG CAGCAGCAGC 
GACTCTGCGC CCGACTGCTG GGACCAGACG GATATGGAGG CCCCCGGGCC CGGGCCTTGC 
GGCGGCGGCG GGAGCGGCAG CGGCTCCATG GCCGCGGTGG CCGAGGCCCA GCGTGAGAAC 
CTCAGCGCGG CTTTCAGCCG GCAACTCAAC GTCAACGCCA AGCCTTTCGT CCCCAACGTC 
CACGCCGCTG AATTCGTGCC GTCCTTCCTG CGGGGTCCGG CCCAGCCGCC GCTATCCCCA 
GCTGGCGCAG CCGGAGGCGA CCACGGAGCA GGCAGCGGCG CGGGAGGCCC TTCGGAACCT 
GTAGAGTCCT CTCAAGACCA GTCGTGTGAA GGTTCAAATT CTACTGTTAG CATGGAACTT 
TCAGAACCTG TTGAAAATGG AGAGACAGAA ATGTCCCCAG AAGAATCATG GGAGCACAAA 
GAAGAAATAA GTGAAGCGGA GCCAGGGGGT GGTTCCTCAG GAGATGGAAG GCCACCAGAG 
GAAAGCACCC AAGAAATGAT GGAGGAGGAG GAAGAAATAC CGAAACCTAA GTCAGCGGTG 
GCACCACCAG GTGCTCCCAA GAAGGAACAT GTAAATGTAG TGTTCATCGG ACATGTTGAT 
GCAGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT CGACAAAAGG 
ACACTTGAGA AGTATGAACG AGAAGCTAAA GAAAAAAACA GAGAAACTTG GTACTTGTCT 
TGGGCCTTAG ACACGAATCA GGAAGAACGA GACAAGGGCA AAACCGTGGA GGTGGGTCGT 
GCCTATTTTG AAACGGAAAA GAAGCATTTT ACAATTCTAG ATGCTCCTGG CCATAAGAGT 
TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGACT TGGCTGTACT GGTAATCTCA 
GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG AGAACATGCA 
ATGTTGGCAA AGACAGCAGG TGTCAAACAC TTAATTGTGC TTATTAATAA GATGGATGAT 
CCAACGGTAA ACTGGAGCAA CGAGAGATAT GAAGAATGTA AAGAGAAACT AGTACCATTT 
TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCATT TTATGCCGTG CTCAGGACTG 
ACAGGAGCGA ATCTTAAAGA ACAATCAGAT TTCTGTCCTT GGTACATTGG CTTACCATTT 
ATTCCATATC TGGATAATTT GCCAAACTTC AATAGATCAG TTGATGGACC AATCAGGCTG 
CCAATTGTCG ATAAGTACAA GGATATGGGG ACTGTGGTCC TGGGAAAGCT GGAATCTGGA 
TCTATTTGTA AAGGCCAGCA GCTTGTAATG ATGCCAAATA AGCACAACGT GGAAGTTCTT 
GGAATACTTT CTGATGATGT AGAAACGGAC TCTGTAGCTC CAGGTGAAAA CCTCAAAATC 
AGACTGAAAG GAATTGAAGA GGAAGAGATT CTTCCAGGAT TCATCCTTTG TGATCTTAAT 
AATCTTTGTC ATTCTGGACG CACATTTGAT GCTCAGATAG TGATTATAGA GCACAAGTCC 
ATCATCTGCC CAGGGTATAA TGCGGTGCTG CATATTCATA CCTGTATCGA AGAAGTCGAA 
ATAACAGCCT TAATCTGCTT GGTAGACAAG AAATCAGGAG AGAAAAGTAA GACTCGACCC 
CGTTTTGTAA AACAAGATCA AGTATGCATT GCGCGTTTAA GGACAGCAGG AACCATCTGC 
CTGGAGACCT TTAAGGACTT CCCTCAGATG GGACGTTTTA CCTTAAGAGA TGAGGGTAAG 
ACCATTGCAA TTGGAAAAGT TCTGAAACTG GTTCCAGAGA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury.
The Journal of biological chemistry292(4)1240-1250(2017 01)
Ishii T,Ueyama T,Shigyo M,Kohta M,Kondoh T,Kuboyama T,Uebi T,Hamada T,Gutmann DH,Aiba A,Kohmura E,Tohda C,Saito N

Functional role of Tia1/Pub1 and Sup35 prion domains: directing protein synthesis machinery to the tubulin cytoskeleton.
Molecular cell55(2)305-18(2014 Jul)
Li X,Rayman JB,Kandel ER,Derkatch IL

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
FEBS letters582(16)2352-6(2008 Jul)
Figaro S,Scrima N,Buckingham RH,Heurgué-Hamard V

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Molecular cloning of a novel member of the eukaryotic polypeptide chain-releasing factors (eRF). Its identification as eRF3 interacting with eRF1.
The Journal of biological chemistry273(35)22254-9(1998 Aug)
Hoshino S,Imai M,Mizutani M,Kikuchi Y,Hanaoka F,Ui M,Katada T

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Gspt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM