Cacna1s cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1s
Entrez Gene ID	12292
Full Name	calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms	AW493108,Cav1.1,Cchl1a3,DHPR,fmd,mdg,sj
General protein information	Preferred Names calcium channel, voltage-dependent, L type, alpha 1S subunit Names voltage-dependent L-type calcium channel subunit alpha-1S DHPR alpha1s calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle dihydropyridine receptor alpha 1S muscle dysgenesis voltage-gated calcium channel subunit alpha Cav1.1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 59.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529107.1	XP_006529170.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
NM_014193.3	NP_055008.2	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
XM_006529106.2	XP_006529169.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
NM_014193.2	NP_055008.2	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
NM_001081023.1	NP_001074492.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
NM_001081023.2	NP_001074492.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
XM_006529106.3	XP_006529169.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CACNA1S	XP_002802046.1
Rattus norvegicus (Norway rat)	Cacna1s	NP_446325.1
Homo sapiens (human)	CACNA1S	NP_000060.2
Pan troglodytes (chimpanzee)	CACNA1S	XP_525018.3
Canis lupus familiaris (dog)	CACNA1S	XP_848685.3
Mus musculus (house mouse)	Cacna1s	NP_001074492.1
Bos taurus (cattle)	CACNA1S	XP_002694288.2
Danio rerio (zebrafish)	cacna1sa	NP_001139622.1
Gallus gallus (chicken)	CACNA1S	XP_419259.3
Danio rerio (zebrafish)	cacna1sb	NP_999891.1

Gene Ontology
Bibliography

Related articles in PubMed

Common cellular origin and diverging developmental programs for different sesamoid bones.
Eyal S, Rubin S, Krief S, Levin L, Zelzer E
Development (Cambridge, England)146(4)(2019 02)

STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor.
Campiglio M, Kaplan MM, Flucher BE
Journal of cellular physiology233(12)9045-9051(2018 12)

Fibromodulin modulates myoblast differentiation by controlling calcium channel.
Lee EJ, Nam JH, Choi I
Biochemical and biophysical research communications503(2)580-585(2018 09)

Calcium Influx and Release Cooperatively Regulate AChR Patterning and Motor Axon Outgrowth during Neuromuscular Junction Formation.
Kaplan MM, Sultana N, Benedetti A, Obermair GJ, Linde NF, Papadopoulos S, Dayal A, Grabner M, Flucher BE
Cell reports23(13)3891-3904(2018 06)

Physical interaction of junctophilin and the Ca
Nakada T, Kashihara T, Komatsu M, Kojima K, Takeshita T, Yamada M
Proceedings of the National Academy of Sciences of the United States of America115(17)4507-4512(2018 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cacna1s gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1s cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27883	XM_006529107.1 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu20380	NM_014193.3 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20467	NM_001081023.2 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27882	XM_006529106.3 Latest version!	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu20380	NM_014193.2	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20467	NM_001081023.1	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27882	XM_006529106.2	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1s ( XM_006529107.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529170.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu27883
Clone ID Related Accession (Same CDS sequence)	XM_006529107.1
Accession Version	XM_006529107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGCA AAACTATCAG CAAGCATAGA CAAAGGGATG AAGAATTAGG AGAGCAACAG 
AGATACAAAC ACAAAGAACG AGCCCTCTCT GCAAGTAAAC AGTCACGGCA GGCCAGGAAA 
GAGAAACTAA GACAGGACAA TGCTCCACAA GGCAGTGGCA GAACCAGGCC CAGAGACCGC 
ATCTCTGTAG CAACCAACTA CTGTTTAAGA TGTCGGGACG ATGAGGAAGA TGAGCCCGAG 
ATCCCAGTGA GCCCCCGACC ACGCCCTCTG GCCGAGCTGC AGCTGAAAGA GAAAGCAGTG 
CCCATCCCGG AGGCTAGCTC CTTCTTCATC TTCAGTCCCA CCAACAAGAT CCGTGTCCTG 
TGTCACCGCA TTGTCAATGC CACCTGGTTC ACCAACTTCA TCCTGCTCTT CATCCTGCTC 
AGCAGTGCCG CCCTGGCCGC TGAGGATCCC ATCCGGGCCG ACTCCATGAG GAATCAGATC 
CTCGAGTATT TTGATTATGT GTTCACTGCT GTTTTCACGG TGGAGATTGT CCTTAAGATG 
ACCACCTATG GAGCCTTCCT GCACAAAGGC TCCTTCTGCC GCAACTACTT CAACATCCTG 
GACCTGCTGG TGGTGGCTGT GTCCCTCATC TCCATGGGCC TCGAGTCCAG CGCCATCTCC 
GTGGTGAAGA TCTTAAGGGT TCTGAGAGTG CTGAGACCAC TCCGGGCCAT AAACAGAGCC 
AAAGGGTTGA AGCACGTGGT GCAGTGCGTG TTTGTTGCCA TTCGCACCAT CGGGAACATC 
GTGCTGGTCA CCACGCTCCT GCAGTTCATG TTTGCGTGCA TTGGAGTGCA GCTTTTCAAG 
GGAAAGTTCT ACAGCTGCAA TGACTTATCC AAGATGACTG AAGAGGAATG CAGGGGCTAC 
TACTACATAT ACAAGGATGG AGACCCCACT CAGATAGAGC TGCGTCCCCG CCAGTGGATA 
CACAATGACT TCCACTTTGA CAATGTACTC TCCGCTATGA TGTCACTCTT CACGGTCTCC 
ACCTTCGAGG GTTGGCCTCA GCTACTGTAC AAGGCCATAG ACTCAAATGA GGAGGACACC 
GGCCCTGTCT ACAACAACCG AGTGGAGATG GCCATCTTCT TCATTATCTA CATCATCCTC 
ATTGCCTTCT TCATGATGAA CATCTTTGTG GGCTTTGTCA TCGTTACCTT CCAGGAACAG 
GGAGAGACTG AGTATAAGAA CTGTGAGCTC GACAAGAACC AGCGCCAATG CGTACAGTAT 
GCCCTGAAGG CCCGCCCACT GAGGTGTTAC ATCCCCAAAA ACCCATACCA GTATCAGGTG 
TGGTATGTCG TCACTTCCTC CTACTTTGAA TACCTGATGT TTGCTCTCAT CATGCTCAAT 
ACTATCTGCC TGGGCATGCA GCATTACAAC CAGTCGGAAC AGATGAACCA CATCTCAGAC 
ATTCTCAACG TGGCTTTCAC CATCATCTTC ACCCTGGAGA TGGTCCTCAA GCTCATAGCT 
TTCAAGCCCA GGGGCTATTT TGGAGATCCT TGGAATGTGT TTGACTTCCT AATCGTCATC 
GGCAGCATCA TTGACGTCAT CCTGAGCGAG ATCGATACTT TCCTGGCCTC CAGCGGGGGA 
CTGTATTGCC TGGGTGGAGG CTGCGGGAAC GTTGACCCAG ATGAGAGCGC CCGTATCTCC 
AGTGCCTTCT TCCGCCTGTT CCGGGTCATG AGACTGGTCA AGCTGCTGAA CCGGGCGGAG 
GGTGTGCGCA CCCTGCTGTG GACATTCATC AAGTCCTTCC AGGCCCTGCC GTACGTGGCT 
TTGCTGATCG TGATGCTCTT CTTCATCTAC GCGGTCATCG GCATGCAGAT GTTCGGGAAG 
ATCGCCATGG TGGACGGGAC GCAGATAAAC CGGAACAACA ACTTCCAGAC TTTCCCACAG 
GCCGTGCTGC TGCTCTTCAG GTGTGCCACA GGGGAAGCCT GGCAAGAGAT CCTGCTGGCC 
TGCAGCTATG GGAAGCTGTG TGACCCGGAG TCTGACTACG CACCCGGGGA GGAGCATACA 
TGCGGCACCA ACTTCGCCTA CTACTACTTC ATCAGTTTCT ACATGCTCTG CGCCTTCCTG 
ATCATCAACC TCTTTGTGGC TGTCATCATG GACAACTTTG ATTACCTCAC AAGGGACTGG 
TCCATTCTGG GCCCTCACCA CCTGGACGAG TTCAAGGCCA TTTGGGCTGA GTACGACCCG 
GAGGCTAAGG GCAGGATCAA GCACCTGGAT GTGGTGACCC TGCTGAGGAG GATTCAGCCC 
CCTCTGGGCT TTGGGAAGTT CTGCCCACAC AGGGTAGCAT GTAAGAGGCT GGTGGGCATG 
AACATGCCCC TGAACAGCGA TGGCACAGTC ACCTTTAATG CCACACTATT TGCCCTGGTG 
CGCACAGCCC TCAAGATCAA GACAGAAGGC AACTTTGAAC AAGCTAACGA GGAGCTGAGA 
GCCATCATCA AGAAGATCTG GAAGAGAACC AGCATGAAGC TCTTGGACCA AGTCATCCCT 
CCAATAGGAG ATGATGAGGT GACAGTAGGG AAATTCTATG CCACATTCCT CATCCAGGAG 
CACTTCCGGA AGTTCATGAA GCGCCAGGAG GAATATTATG GGTATCGACC CAAAAAGGAC 
ACAGTTCAGA TACAGGCTGG ATTACGGACC ATAGAGGAAG AGGCAGCTCC TGAGATCCAC 
CGTGCCATCT CTGGAGACCT GACAGCAGAG GAGGAACTGG AGAGGGCCAT GGTAGAGGCT 
GCCATGGAGG AGGGGATATT CCGGAGGACG GGAGGCCTGT TTGGTCAAGT GGACAACTTC 
CTGGAAAGGA CCAACTCTCT GCCCCCTGTC ATGGCCAACC AAAGACCCCT CCAATTTGCT 
GAAATAGAGA TGGAAGAGCT GGAGTCACCA GTCTTCTTGG AGGACTTCCC TCAGAACCCA 
GGCACCCACC CTCTTGCTCG TGCCAATACC AACAACGCCA ATGCCAATGT TGCCTATGGC 
AACAGCAGCC ATAGAAACAA CCCAGTGTTT TCCAGCATCT GCTATGAACG GGAGTTCTTG 
GGAGAGGCGG ACATGCCTGT TACCAGAGAA GGACCCCTCA GTCAGCCCTG CAGGGCCTCA 
GGACCTCACA GCAGATCTCA TGTGGACAAA CTGAAAAGAC CGATGACCCA GAGAGGAATG 
CCTGAAGGCC AGGTGCCTCC TTCCCCGTGC CAGCTCTCCC AGGCAGAGCA TCCTGTGCAG 
AAAGAGGGAA AGGGCCCCAC GTCACGGTTT CTGGAAACAC CCAACAGCAG GAACTTTGAG 
GAGCACGTTC CCAGGAATTC GGCTCACAGG TGCACGGCTC CAGCTACTGC CATGCTGATC 
CAGGAGGCTC TGGTTCGAGG AGGACTAGAC TCCTTAGCAG CAGATGCTAA CTTCGTCATG 
GCAACAGGCC AGGCCCTGGC AGATGCCTGC CAGATGGAAC CAGAGGAAGT CGAGGTGGCA 
GCAACAGAGC TACTGAAGCA AGAATCCCCA GAGGGCGGGG CCGTGCCCTG GGAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529170.1
CDS	9..3548
Translation	MYSKTISKHRQRDEELGEQQRYKHKERALSASKQSRQARKEKLRQDNAPQGSGRTRPRDRISVATNYCLRCRDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	XM_006529107.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529107.1

ORF Insert Sequence:

ATGTACAGCA AAACTATCAG CAAGCATAGA CAAAGGGATG AAGAATTAGG AGAGCAACAG 
AGATACAAAC ACAAAGAACG AGCCCTCTCT GCAAGTAAAC AGTCACGGCA GGCCAGGAAA 
GAGAAACTAA GACAGGACAA TGCTCCACAA GGCAGTGGCA GAACCAGGCC CAGAGACCGC 
ATCTCTGTAG CAACCAACTA CTGTTTAAGA TGTCGGGACG ATGAGGAAGA TGAGCCCGAG 
ATCCCAGTGA GCCCCCGACC ACGCCCTCTG GCCGAGCTGC AGCTGAAAGA GAAAGCAGTG 
CCCATCCCGG AGGCTAGCTC CTTCTTCATC TTCAGTCCCA CCAACAAGAT CCGTGTCCTG 
TGTCACCGCA TTGTCAATGC CACCTGGTTC ACCAACTTCA TCCTGCTCTT CATCCTGCTC 
AGCAGTGCCG CCCTGGCCGC TGAGGATCCC ATCCGGGCCG ACTCCATGAG GAATCAGATC 
CTCGAGTATT TTGATTATGT GTTCACTGCT GTTTTCACGG TGGAGATTGT CCTTAAGATG 
ACCACCTATG GAGCCTTCCT GCACAAAGGC TCCTTCTGCC GCAACTACTT CAACATCCTG 
GACCTGCTGG TGGTGGCTGT GTCCCTCATC TCCATGGGCC TCGAGTCCAG CGCCATCTCC 
GTGGTGAAGA TCTTAAGGGT TCTGAGAGTG CTGAGACCAC TCCGGGCCAT AAACAGAGCC 
AAAGGGTTGA AGCACGTGGT GCAGTGCGTG TTTGTTGCCA TTCGCACCAT CGGGAACATC 
GTGCTGGTCA CCACGCTCCT GCAGTTCATG TTTGCGTGCA TTGGAGTGCA GCTTTTCAAG 
GGAAAGTTCT ACAGCTGCAA TGACTTATCC AAGATGACTG AAGAGGAATG CAGGGGCTAC 
TACTACATAT ACAAGGATGG AGACCCCACT CAGATAGAGC TGCGTCCCCG CCAGTGGATA 
CACAATGACT TCCACTTTGA CAATGTACTC TCCGCTATGA TGTCACTCTT CACGGTCTCC 
ACCTTCGAGG GTTGGCCTCA GCTACTGTAC AAGGCCATAG ACTCAAATGA GGAGGACACC 
GGCCCTGTCT ACAACAACCG AGTGGAGATG GCCATCTTCT TCATTATCTA CATCATCCTC 
ATTGCCTTCT TCATGATGAA CATCTTTGTG GGCTTTGTCA TCGTTACCTT CCAGGAACAG 
GGAGAGACTG AGTATAAGAA CTGTGAGCTC GACAAGAACC AGCGCCAATG CGTACAGTAT 
GCCCTGAAGG CCCGCCCACT GAGGTGTTAC ATCCCCAAAA ACCCATACCA GTATCAGGTG 
TGGTATGTCG TCACTTCCTC CTACTTTGAA TACCTGATGT TTGCTCTCAT CATGCTCAAT 
ACTATCTGCC TGGGCATGCA GCATTACAAC CAGTCGGAAC AGATGAACCA CATCTCAGAC 
ATTCTCAACG TGGCTTTCAC CATCATCTTC ACCCTGGAGA TGGTCCTCAA GCTCATAGCT 
TTCAAGCCCA GGGGCTATTT TGGAGATCCT TGGAATGTGT TTGACTTCCT AATCGTCATC 
GGCAGCATCA TTGACGTCAT CCTGAGCGAG ATCGATACTT TCCTGGCCTC CAGCGGGGGA 
CTGTATTGCC TGGGTGGAGG CTGCGGGAAC GTTGACCCAG ATGAGAGCGC CCGTATCTCC 
AGTGCCTTCT TCCGCCTGTT CCGGGTCATG AGACTGGTCA AGCTGCTGAA CCGGGCGGAG 
GGTGTGCGCA CCCTGCTGTG GACATTCATC AAGTCCTTCC AGGCCCTGCC GTACGTGGCT 
TTGCTGATCG TGATGCTCTT CTTCATCTAC GCGGTCATCG GCATGCAGAT GTTCGGGAAG 
ATCGCCATGG TGGACGGGAC GCAGATAAAC CGGAACAACA ACTTCCAGAC TTTCCCACAG 
GCCGTGCTGC TGCTCTTCAG GTGTGCCACA GGGGAAGCCT GGCAAGAGAT CCTGCTGGCC 
TGCAGCTATG GGAAGCTGTG TGACCCGGAG TCTGACTACG CACCCGGGGA GGAGCATACA 
TGCGGCACCA ACTTCGCCTA CTACTACTTC ATCAGTTTCT ACATGCTCTG CGCCTTCCTG 
ATCATCAACC TCTTTGTGGC TGTCATCATG GACAACTTTG ATTACCTCAC AAGGGACTGG 
TCCATTCTGG GCCCTCACCA CCTGGACGAG TTCAAGGCCA TTTGGGCTGA GTACGACCCG 
GAGGCTAAGG GCAGGATCAA GCACCTGGAT GTGGTGACCC TGCTGAGGAG GATTCAGCCC 
CCTCTGGGCT TTGGGAAGTT CTGCCCACAC AGGGTAGCAT GTAAGAGGCT GGTGGGCATG 
AACATGCCCC TGAACAGCGA TGGCACAGTC ACCTTTAATG CCACACTATT TGCCCTGGTG 
CGCACAGCCC TCAAGATCAA GACAGAAGGC AACTTTGAAC AAGCTAACGA GGAGCTGAGA 
GCCATCATCA AGAAGATCTG GAAGAGAACC AGCATGAAGC TCTTGGACCA AGTCATCCCT 
CCAATAGGAG ATGATGAGGT GACAGTAGGG AAATTCTATG CCACATTCCT CATCCAGGAG 
CACTTCCGGA AGTTCATGAA GCGCCAGGAG GAATATTATG GGTATCGACC CAAAAAGGAC 
ACAGTTCAGA TACAGGCTGG ATTACGGACC ATAGAGGAAG AGGCAGCTCC TGAGATCCAC 
CGTGCCATCT CTGGAGACCT GACAGCAGAG GAGGAACTGG AGAGGGCCAT GGTAGAGGCT 
GCCATGGAGG AGGGGATATT CCGGAGGACG GGAGGCCTGT TTGGTCAAGT GGACAACTTC 
CTGGAAAGGA CCAACTCTCT GCCCCCTGTC ATGGCCAACC AAAGACCCCT CCAATTTGCT 
GAAATAGAGA TGGAAGAGCT GGAGTCACCA GTCTTCTTGG AGGACTTCCC TCAGAACCCA 
GGCACCCACC CTCTTGCTCG TGCCAATACC AACAACGCCA ATGCCAATGT TGCCTATGGC 
AACAGCAGCC ATAGAAACAA CCCAGTGTTT TCCAGCATCT GCTATGAACG GGAGTTCTTG 
GGAGAGGCGG ACATGCCTGT TACCAGAGAA GGACCCCTCA GTCAGCCCTG CAGGGCCTCA 
GGACCTCACA GCAGATCTCA TGTGGACAAA CTGAAAAGAC CGATGACCCA GAGAGGAATG 
CCTGAAGGCC AGGTGCCTCC TTCCCCGTGC CAGCTCTCCC AGGCAGAGCA TCCTGTGCAG 
AAAGAGGGAA AGGGCCCCAC GTCACGGTTT CTGGAAACAC CCAACAGCAG GAACTTTGAG 
GAGCACGTTC CCAGGAATTC GGCTCACAGG TGCACGGCTC CAGCTACTGC CATGCTGATC 
CAGGAGGCTC TGGTTCGAGG AGGACTAGAC TCCTTAGCAG CAGATGCTAA CTTCGTCATG 
GCAACAGGCC AGGCCCTGGC AGATGCCTGC CAGATGGAAC CAGAGGAAGT CGAGGTGGCA 
GCAACAGAGC TACTGAAGCA AGAATCCCCA GAGGGCGGGG CCGTGCCCTG GGAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( NM_014193.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_055008.2 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20380
Clone ID Related Accession (Same CDS sequence)	NM_014193.3 , NM_014193.2
Accession Version	NM_014193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124375.5 and AC124550.4. On Feb 22, 2019 this sequence version replaced NM_014193.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATG ACCCAGATGA GAGCGCCCGT ATCTCCAGTG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGAC TGGTCAAGCT GCTGAACCGG GCGGAGGGTG TGCGCACCCT GCTGTGGACA 
TTCATCAAGT CCTTCCAGGC CCTGCCGTAC GTGGCTTTGC TGATCGTGAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCATGGTGGA CGGGACGCAG 
ATAAACCGGA ACAACAACTT CCAGACTTTC CCACAGGCCG TGCTGCTGCT CTTCAGGTGT 
GCCACAGGGG AAGCCTGGCA AGAGATCCTG CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCGGAGTCTG ACTACGCACC CGGGGAGGAG CATACATGCG GCACCAACTT CGCCTACTAC 
TACTTCATCA GTTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ACTTTGATTA CCTCACAAGG GACTGGTCCA TTCTGGGCCC TCACCACCTG 
GACGAGTTCA AGGCCATTTG GGCTGAGTAC GACCCGGAGG CTAAGGGCAG GATCAAGCAC 
CTGGATGTGG TGACCCTGCT GAGGAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACACAGGG TAGCATGTAA GAGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACAGTCACCT TTAATGCCAC ACTATTTGCC CTGGTGCGCA CAGCCCTCAA GATCAAGACA 
GAAGGCAACT TTGAACAAGC TAACGAGGAG CTGAGAGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAAGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTAGGGAAAT TCTATGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAGGAAT ATTATGGGTA TCGACCCAAA AAGGACACAG TTCAGATACA GGCTGGATTA 
CGGACCATAG AGGAAGAGGC AGCTCCTGAG ATCCACCGTG CCATCTCTGG AGACCTGACA 
GCAGAGGAGG AACTGGAGAG GGCCATGGTA GAGGCTGCCA TGGAGGAGGG GATATTCCGG 
AGGACGGGAG GCCTGTTTGG TCAAGTGGAC AACTTCCTGG AAAGGACCAA CTCTCTGCCC 
CCTGTCATGG CCAACCAAAG ACCCCTCCAA TTTGCTGAAA TAGAGATGGA AGAGCTGGAG 
TCACCAGTCT TCTTGGAGGA CTTCCCTCAG AACCCAGGCA CCCACCCTCT TGCTCGTGCC 
AATACCAACA ACGCCAATGC CAATGTTGCC TATGGCAACA GCAGCCATAG AAACAACCCA 
GTGTTTTCCA GCATCTGCTA TGAACGGGAG TTCTTGGGAG AGGCGGACAT GCCTGTTACC 
AGAGAAGGAC CCCTCAGTCA GCCCTGCAGG GCCTCAGGAC CTCACAGCAG ATCTCATGTG 
GACAAACTGA AAAGACCGAT GACCCAGAGA GGAATGCCTG AAGGCCAGGT GCCTCCTTCC 
CCGTGCCAGC TCTCCCAGGC AGAGCATCCT GTGCAGAAAG AGGGAAAGGG CCCCACGTCA 
CGGTTTCTGG AAACACCCAA CAGCAGGAAC TTTGAGGAGC ACGTTCCCAG GAATTCGGCT 
CACAGGTGCA CGGCTCCAGC TACTGCCATG CTGATCCAGG AGGCTCTGGT TCGAGGAGGA 
CTAGACTCCT TAGCAGCAGA TGCTAACTTC GTCATGGCAA CAGGCCAGGC CCTGGCAGAT 
GCCTGCCAGA TGGAACCAGA GGAAGTCGAG GTGGCAGCAA CAGAGCTACT GAAGCAAGAA 
TCCCCAGAGG GCGGGGCCGT GCCCTGGGAG CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055008.2
CDS	91..5586
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	NM_014193.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014193.3

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATG ACCCAGATGA GAGCGCCCGT ATCTCCAGTG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGAC TGGTCAAGCT GCTGAACCGG GCGGAGGGTG TGCGCACCCT GCTGTGGACA 
TTCATCAAGT CCTTCCAGGC CCTGCCGTAC GTGGCTTTGC TGATCGTGAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCATGGTGGA CGGGACGCAG 
ATAAACCGGA ACAACAACTT CCAGACTTTC CCACAGGCCG TGCTGCTGCT CTTCAGGTGT 
GCCACAGGGG AAGCCTGGCA AGAGATCCTG CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCGGAGTCTG ACTACGCACC CGGGGAGGAG CATACATGCG GCACCAACTT CGCCTACTAC 
TACTTCATCA GTTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ACTTTGATTA CCTCACAAGG GACTGGTCCA TTCTGGGCCC TCACCACCTG 
GACGAGTTCA AGGCCATTTG GGCTGAGTAC GACCCGGAGG CTAAGGGCAG GATCAAGCAC 
CTGGATGTGG TGACCCTGCT GAGGAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACACAGGG TAGCATGTAA GAGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACAGTCACCT TTAATGCCAC ACTATTTGCC CTGGTGCGCA CAGCCCTCAA GATCAAGACA 
GAAGGCAACT TTGAACAAGC TAACGAGGAG CTGAGAGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAAGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTAGGGAAAT TCTATGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAGGAAT ATTATGGGTA TCGACCCAAA AAGGACACAG TTCAGATACA GGCTGGATTA 
CGGACCATAG AGGAAGAGGC AGCTCCTGAG ATCCACCGTG CCATCTCTGG AGACCTGACA 
GCAGAGGAGG AACTGGAGAG GGCCATGGTA GAGGCTGCCA TGGAGGAGGG GATATTCCGG 
AGGACGGGAG GCCTGTTTGG TCAAGTGGAC AACTTCCTGG AAAGGACCAA CTCTCTGCCC 
CCTGTCATGG CCAACCAAAG ACCCCTCCAA TTTGCTGAAA TAGAGATGGA AGAGCTGGAG 
TCACCAGTCT TCTTGGAGGA CTTCCCTCAG AACCCAGGCA CCCACCCTCT TGCTCGTGCC 
AATACCAACA ACGCCAATGC CAATGTTGCC TATGGCAACA GCAGCCATAG AAACAACCCA 
GTGTTTTCCA GCATCTGCTA TGAACGGGAG TTCTTGGGAG AGGCGGACAT GCCTGTTACC 
AGAGAAGGAC CCCTCAGTCA GCCCTGCAGG GCCTCAGGAC CTCACAGCAG ATCTCATGTG 
GACAAACTGA AAAGACCGAT GACCCAGAGA GGAATGCCTG AAGGCCAGGT GCCTCCTTCC 
CCGTGCCAGC TCTCCCAGGC AGAGCATCCT GTGCAGAAAG AGGGAAAGGG CCCCACGTCA 
CGGTTTCTGG AAACACCCAA CAGCAGGAAC TTTGAGGAGC ACGTTCCCAG GAATTCGGCT 
CACAGGTGCA CGGCTCCAGC TACTGCCATG CTGATCCAGG AGGCTCTGGT TCGAGGAGGA 
CTAGACTCCT TAGCAGCAGA TGCTAACTTC GTCATGGCAA CAGGCCAGGC CCTGGCAGAT 
GCCTGCCAGA TGGAACCAGA GGAAGTCGAG GTGGCAGCAA CAGAGCTACT GAAGCAAGAA 
TCCCCAGAGG GCGGGGCCGT GCCCTGGGAG CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( NM_014193.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_055008.2 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.

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CloneID	OMu20380
Clone ID Related Accession (Same CDS sequence)	NM_014193.3 , NM_014193.2
Accession Version	NM_014193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545408000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124375.5, AK143541.1 and AC124550.4. On Jun 5, 2008 this sequence version replaced NM_014193.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATG ACCCAGATGA GAGCGCCCGT ATCTCCAGTG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGAC TGGTCAAGCT GCTGAACCGG GCGGAGGGTG TGCGCACCCT GCTGTGGACA 
TTCATCAAGT CCTTCCAGGC CCTGCCGTAC GTGGCTTTGC TGATCGTGAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCATGGTGGA CGGGACGCAG 
ATAAACCGGA ACAACAACTT CCAGACTTTC CCACAGGCCG TGCTGCTGCT CTTCAGGTGT 
GCCACAGGGG AAGCCTGGCA AGAGATCCTG CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCGGAGTCTG ACTACGCACC CGGGGAGGAG CATACATGCG GCACCAACTT CGCCTACTAC 
TACTTCATCA GTTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ACTTTGATTA CCTCACAAGG GACTGGTCCA TTCTGGGCCC TCACCACCTG 
GACGAGTTCA AGGCCATTTG GGCTGAGTAC GACCCGGAGG CTAAGGGCAG GATCAAGCAC 
CTGGATGTGG TGACCCTGCT GAGGAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACACAGGG TAGCATGTAA GAGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACAGTCACCT TTAATGCCAC ACTATTTGCC CTGGTGCGCA CAGCCCTCAA GATCAAGACA 
GAAGGCAACT TTGAACAAGC TAACGAGGAG CTGAGAGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAAGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTAGGGAAAT TCTATGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAGGAAT ATTATGGGTA TCGACCCAAA AAGGACACAG TTCAGATACA GGCTGGATTA 
CGGACCATAG AGGAAGAGGC AGCTCCTGAG ATCCACCGTG CCATCTCTGG AGACCTGACA 
GCAGAGGAGG AACTGGAGAG GGCCATGGTA GAGGCTGCCA TGGAGGAGGG GATATTCCGG 
AGGACGGGAG GCCTGTTTGG TCAAGTGGAC AACTTCCTGG AAAGGACCAA CTCTCTGCCC 
CCTGTCATGG CCAACCAAAG ACCCCTCCAA TTTGCTGAAA TAGAGATGGA AGAGCTGGAG 
TCACCAGTCT TCTTGGAGGA CTTCCCTCAG AACCCAGGCA CCCACCCTCT TGCTCGTGCC 
AATACCAACA ACGCCAATGC CAATGTTGCC TATGGCAACA GCAGCCATAG AAACAACCCA 
GTGTTTTCCA GCATCTGCTA TGAACGGGAG TTCTTGGGAG AGGCGGACAT GCCTGTTACC 
AGAGAAGGAC CCCTCAGTCA GCCCTGCAGG GCCTCAGGAC CTCACAGCAG ATCTCATGTG 
GACAAACTGA AAAGACCGAT GACCCAGAGA GGAATGCCTG AAGGCCAGGT GCCTCCTTCC 
CCGTGCCAGC TCTCCCAGGC AGAGCATCCT GTGCAGAAAG AGGGAAAGGG CCCCACGTCA 
CGGTTTCTGG AAACACCCAA CAGCAGGAAC TTTGAGGAGC ACGTTCCCAG GAATTCGGCT 
CACAGGTGCA CGGCTCCAGC TACTGCCATG CTGATCCAGG AGGCTCTGGT TCGAGGAGGA 
CTAGACTCCT TAGCAGCAGA TGCTAACTTC GTCATGGCAA CAGGCCAGGC CCTGGCAGAT 
GCCTGCCAGA TGGAACCAGA GGAAGTCGAG GTGGCAGCAA CAGAGCTACT GAAGCAAGAA 
TCCCCAGAGG GCGGGGCCGT GCCCTGGGAG CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055008.2
CDS	1..5496
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	NM_014193.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014193.2

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATG ACCCAGATGA GAGCGCCCGT ATCTCCAGTG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGAC TGGTCAAGCT GCTGAACCGG GCGGAGGGTG TGCGCACCCT GCTGTGGACA 
TTCATCAAGT CCTTCCAGGC CCTGCCGTAC GTGGCTTTGC TGATCGTGAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCATGGTGGA CGGGACGCAG 
ATAAACCGGA ACAACAACTT CCAGACTTTC CCACAGGCCG TGCTGCTGCT CTTCAGGTGT 
GCCACAGGGG AAGCCTGGCA AGAGATCCTG CTGGCCTGCA GCTATGGGAA GCTGTGTGAC 
CCGGAGTCTG ACTACGCACC CGGGGAGGAG CATACATGCG GCACCAACTT CGCCTACTAC 
TACTTCATCA GTTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCTGTC 
ATCATGGACA ACTTTGATTA CCTCACAAGG GACTGGTCCA TTCTGGGCCC TCACCACCTG 
GACGAGTTCA AGGCCATTTG GGCTGAGTAC GACCCGGAGG CTAAGGGCAG GATCAAGCAC 
CTGGATGTGG TGACCCTGCT GAGGAGGATT CAGCCCCCTC TGGGCTTTGG GAAGTTCTGC 
CCACACAGGG TAGCATGTAA GAGGCTGGTG GGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACAGTCACCT TTAATGCCAC ACTATTTGCC CTGGTGCGCA CAGCCCTCAA GATCAAGACA 
GAAGGCAACT TTGAACAAGC TAACGAGGAG CTGAGAGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCTT GGACCAAGTC ATCCCTCCAA TAGGAGATGA TGAGGTGACA 
GTAGGGAAAT TCTATGCCAC ATTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAGGAAT ATTATGGGTA TCGACCCAAA AAGGACACAG TTCAGATACA GGCTGGATTA 
CGGACCATAG AGGAAGAGGC AGCTCCTGAG ATCCACCGTG CCATCTCTGG AGACCTGACA 
GCAGAGGAGG AACTGGAGAG GGCCATGGTA GAGGCTGCCA TGGAGGAGGG GATATTCCGG 
AGGACGGGAG GCCTGTTTGG TCAAGTGGAC AACTTCCTGG AAAGGACCAA CTCTCTGCCC 
CCTGTCATGG CCAACCAAAG ACCCCTCCAA TTTGCTGAAA TAGAGATGGA AGAGCTGGAG 
TCACCAGTCT TCTTGGAGGA CTTCCCTCAG AACCCAGGCA CCCACCCTCT TGCTCGTGCC 
AATACCAACA ACGCCAATGC CAATGTTGCC TATGGCAACA GCAGCCATAG AAACAACCCA 
GTGTTTTCCA GCATCTGCTA TGAACGGGAG TTCTTGGGAG AGGCGGACAT GCCTGTTACC 
AGAGAAGGAC CCCTCAGTCA GCCCTGCAGG GCCTCAGGAC CTCACAGCAG ATCTCATGTG 
GACAAACTGA AAAGACCGAT GACCCAGAGA GGAATGCCTG AAGGCCAGGT GCCTCCTTCC 
CCGTGCCAGC TCTCCCAGGC AGAGCATCCT GTGCAGAAAG AGGGAAAGGG CCCCACGTCA 
CGGTTTCTGG AAACACCCAA CAGCAGGAAC TTTGAGGAGC ACGTTCCCAG GAATTCGGCT 
CACAGGTGCA CGGCTCCAGC TACTGCCATG CTGATCCAGG AGGCTCTGGT TCGAGGAGGA 
CTAGACTCCT TAGCAGCAGA TGCTAACTTC GTCATGGCAA CAGGCCAGGC CCTGGCAGAT 
GCCTGCCAGA TGGAACCAGA GGAAGTCGAG GTGGCAGCAA CAGAGCTACT GAAGCAAGAA 
TCCCCAGAGG GCGGGGCCGT GCCCTGGGAG CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( NM_001081023.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074492.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.

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CloneID	OMu20467
Clone ID Related Accession (Same CDS sequence)	NM_001081023.1 , NM_001081023.2
Accession Version	NM_001081023.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545408000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124375.5 and AC124550.4. On Feb 6, 2007 this sequence version replaced XM_001002706.1. Transcript Variant: This variant (2) lacks multiple in-frame segments in the 3' coding region, compared to variant 1, which results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN01164137 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGGTAA CAGGGGCAAA AGCAGAGCAT CCTGTGCAGA AAGAGGGAAA GGGCCCCACG 
TCACGGTTTC TGGAAACACC CAACAGCAGG AACTTTGAGG AGCACGTTCC CAGGAATTCG 
GCTCACAGGT GCACGGCTCC AGCTACTGCC ATGCTGATCC AGGAGGCTCT GGTTCGAGGA 
GGACTAGACT CCTTAGCAGC AGATGCTAAC TTCGTCATGG CAACAGGCCA GGCCCTGGCA 
GATGCCTGCC AGATGGAACC AGAGGAAGTC GAGGTGGCAG CAACAGAGCT ACTGAAGCAA 
GAATCCCCAG AGGGCGGGGC CGTGCCCTGG GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074492.1
CDS	1..5559
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQVTGAKAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	NM_001081023.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081023.1

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGGTAA CAGGGGCAAA AGCAGAGCAT CCTGTGCAGA AAGAGGGAAA GGGCCCCACG 
TCACGGTTTC TGGAAACACC CAACAGCAGG AACTTTGAGG AGCACGTTCC CAGGAATTCG 
GCTCACAGGT GCACGGCTCC AGCTACTGCC ATGCTGATCC AGGAGGCTCT GGTTCGAGGA 
GGACTAGACT CCTTAGCAGC AGATGCTAAC TTCGTCATGG CAACAGGCCA GGCCCTGGCA 
GATGCCTGCC AGATGGAACC AGAGGAAGTC GAGGTGGCAG CAACAGAGCT ACTGAAGCAA 
GAATCCCCAG AGGGCGGGGC CGTGCCCTGG GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( NM_001081023.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074492.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.

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CloneID	OMu20467
Clone ID Related Accession (Same CDS sequence)	NM_001081023.1 , NM_001081023.2
Accession Version	NM_001081023.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124375.5 and AC124550.4. On Feb 22, 2019 this sequence version replaced NM_001081023.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN01164137 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGGTAA CAGGGGCAAA AGCAGAGCAT CCTGTGCAGA AAGAGGGAAA GGGCCCCACG 
TCACGGTTTC TGGAAACACC CAACAGCAGG AACTTTGAGG AGCACGTTCC CAGGAATTCG 
GCTCACAGGT GCACGGCTCC AGCTACTGCC ATGCTGATCC AGGAGGCTCT GGTTCGAGGA 
GGACTAGACT CCTTAGCAGC AGATGCTAAC TTCGTCATGG CAACAGGCCA GGCCCTGGCA 
GATGCCTGCC AGATGGAACC AGAGGAAGTC GAGGTGGCAG CAACAGAGCT ACTGAAGCAA 
GAATCCCCAG AGGGCGGGGC CGTGCCCTGG GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074492.1
CDS	91..5649
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQVTGAKAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	NM_001081023.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081023.2

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGGTAA CAGGGGCAAA AGCAGAGCAT CCTGTGCAGA AAGAGGGAAA GGGCCCCACG 
TCACGGTTTC TGGAAACACC CAACAGCAGG AACTTTGAGG AGCACGTTCC CAGGAATTCG 
GCTCACAGGT GCACGGCTCC AGCTACTGCC ATGCTGATCC AGGAGGCTCT GGTTCGAGGA 
GGACTAGACT CCTTAGCAGC AGATGCTAAC TTCGTCATGG CAACAGGCCA GGCCCTGGCA 
GATGCCTGCC AGATGGAACC AGAGGAAGTC GAGGTGGCAG CAACAGAGCT ACTGAAGCAA 
GAATCCCCAG AGGGCGGGGC CGTGCCCTGG GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( XM_006529106.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529169.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.

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CloneID	OMu27882
Clone ID Related Accession (Same CDS sequence)	XM_006529106.2 , XM_006529106.3
Accession Version	XM_006529106.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGCTCT CCCAGGCAGA GCATCCTGTG CAGAAAGAGG GAAAGGGCCC CACGTCACGG 
TTTCTGGAAA CACCCAACAG CAGGAACTTT GAGGAGCACG TTCCCAGGAA TTCGGCTCAC 
AGGTGCACGG CTCCAGCTAC TGCCATGCTG ATCCAGGAGG CTCTGGTTCG AGGAGGACTA 
GACTCCTTAG CAGCAGATGC TAACTTCGTC ATGGCAACAG GCCAGGCCCT GGCAGATGCC 
TGCCAGATGG AACCAGAGGA AGTCGAGGTG GCAGCAACAG AGCTACTGAA GCAAGAATCC 
CCAGAGGGCG GGGCCGTGCC CTGGGAGCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529169.1
CDS	97..5649
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	XM_006529106.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529106.2

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGCTCT CCCAGGCAGA GCATCCTGTG CAGAAAGAGG GAAAGGGCCC CACGTCACGG 
TTTCTGGAAA CACCCAACAG CAGGAACTTT GAGGAGCACG TTCCCAGGAA TTCGGCTCAC 
AGGTGCACGG CTCCAGCTAC TGCCATGCTG ATCCAGGAGG CTCTGGTTCG AGGAGGACTA 
GACTCCTTAG CAGCAGATGC TAACTTCGTC ATGGCAACAG GCCAGGCCCT GGCAGATGCC 
TGCCAGATGG AACCAGAGGA AGTCGAGGTG GCAGCAACAG AGCTACTGAA GCAAGAATCC 
CCAGAGGGCG GGGCCGTGCC CTGGGAGCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1s ( XM_006529106.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529169.1 Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.

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CloneID	OMu27882
Clone ID Related Accession (Same CDS sequence)	XM_006529106.2 , XM_006529106.3
Accession Version	XM_006529106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGCTCT CCCAGGCAGA GCATCCTGTG CAGAAAGAGG GAAAGGGCCC CACGTCACGG 
TTTCTGGAAA CACCCAACAG CAGGAACTTT GAGGAGCACG TTCCCAGGAA TTCGGCTCAC 
AGGTGCACGG CTCCAGCTAC TGCCATGCTG ATCCAGGAGG CTCTGGTTCG AGGAGGACTA 
GACTCCTTAG CAGCAGATGC TAACTTCGTC ATGGCAACAG GCCAGGCCCT GGCAGATGCC 
TGCCAGATGG AACCAGAGGA AGTCGAGGTG GCAGCAACAG AGCTACTGAA GCAAGAATCC 
CCAGAGGGCG GGGCCGTGCC CTGGGAGCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529169.1
CDS	96..5648
Translation	MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVTREGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRNFEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQESPEGGAVPWEP

Target ORF information:

RefSeq Version	XM_006529106.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529106.3

ORF Insert Sequence:

ATGGAGCCGC CCTCACCCCA GGACGAGGGC CTGAGGAAGA AGCAGCCGAA GAAACCTGTT 
CCTGAGATTC TGCCGAGGCC CCCGCGGGCC TTGTTCTGCC TGACTCTACA GAACCCCCTG 
AGGAAGGCCT GCATCAGCAT CGTGGAATGG AAACCCTTCG AGACCATCAT CTTGCTCACC 
ATCTTTGCCA ACTGTGTGGC CCTGGCAGTG TACCTGCCCA TGCCAGAGGA TGACAACAAC 
ACTCTGAACC TCGGCCTGGA GAAACTGGAG TACTTCTTCC TCATTGTCTT CTCCATTGAA 
GCCGCCATGA AGATCATAGC CTACGGCTTC CTATTCCATC AGGATGCATA CCTGCGCAGT 
GGCTGGAACG TTCTGGACTT CATCATTGTC TTTCTGGGGG TCTTCACTGT GATTCTGGAA 
CAAGTTAACA TCATTCAAAC CAACACGGCC CCCATGAGCA GCAAAGGAGC CGGCTTGGAT 
GTGAAGGCCC TTCGAGCATT CCGCGTGCTC AGACCCCTGC GGCTGGTGTC GGGGGTGCCC 
AGTCTTCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TCCCCCTGTT CCACATTGCC 
CTGCTCGTCC TCTTCATGGT CATCATCTAC GCCATCATTG GGCTGGAGCT CTTCAAAGGC 
AAGATGCACA AGACTTGCTA CTTCATTGGG ACAGACATTG TAGCCACAGT TGAGAATGAG 
AAACCATCAC CCTGTGCTAG AACTGGCTCT GGGCGTCCCT GCACCATCAA TGGCAGCGAG 
TGCCGAGGAG GTTGGCCAGG ACCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCTATGCTCA CTGTGTATCA ATGCATCTCC ATGGAGGGGT GGACTGATGT CCTGTACTGG 
GTCAACGATG CCATCGGGAA TGAGTGGCCC TGGATCTACT TTGTCACCCT CATTCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGTGTCCTGA GTGGAGAATT CACCAAAGAG 
CGAGAGAAGG CCAAGTCCAG GGGAACCTTC CAAAAGCTCC GAGAGAAACA GCAGCTGGAG 
GAGGACCTCC GTGGTTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTGGACGAC 
TTGAGAGAAG GCAAGCTGTC TTTGGATGAA GGGGGATCAG ACACAGAGAG CTTGTATGAA 
ATCGAAGGCT TGAACAAAAT CATCCAGTTC ATTCGGCACT GGCGACAGTG GAATCGCGTC 
TTCCGCTGGA AGTGCCATGA CCTAGTGAAA TCCAAGGTCT TCTATTGGCT GGTGATCCTG 
ATCGTTGCCC TAAACACCCT GTCCATTGCC TCGGAGCACC ACAACCAGCC ACTCTGGTTG 
ACCCATTTGC AAGATGTTGC CAACCGCGTG TTGCTGACCC TCTTCACCAT CGAAATGCTC 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CATCCTGGAG ATCCTGCTGG TGGAGTCGGG AGCCATGTCG 
CCCCTGGGCA TCTCTGTGCT CCGCTGTATC CGCCTCCTGA GGCTCTTCAA GATCACCAAG 
TACTGGACAT CGCTGAGCAA CCTGGTGGCC TCACTGCTCA ACTCCATCCG CTCCATTGCT 
TCCCTCTTGC TGCTGCTCTT TCTCTTCATC ATCATCTTTG CCCTGCTGGG CATGCAGCTC 
TTTGGAGGCA GATATGACTT TGAAGACACA GAAGTACGAC GAAGCAACTT TGACAACTTC 
CCCCAGGCCC TCATCAGTGT CTTCCAGGTC CTGACGGGTG AAGACTGGAA CTCTGTGATG 
TACAATGGGA TCATGGCTTA CGGAGGTCCG ACCTACCCTG GTGTGCTGGT GTGCATTTAT 
TTCATCATCC TTTTCGTCTG TGGCAACTAC ATCCTTCTCA ACGTCTTCCT GGCTATTGCT 
GTGGACAACC TGGCAGAGGC AGAGAGTCTG ACATCTGCCC AAAAGGCCAA GGCTGAGGAG 
AGAAAGCGCA GGAAGATGTC GAAAGGGCTC CCAGACAAGT CAGAAGAGGA GAGGGCCACG 
GTGACCAAGA AACTGGAGCA GAAGTCCAAG GGTGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAAATCGATG AGTTTGAATC AAACGTTAAT GAGGTGAAAG ACCCCTACCC TTCGGCTGAC 
TTCCCAGGGG ACGATGAGGA AGATGAGCCC GAGATCCCAG TGAGCCCCCG ACCACGCCCT 
CTGGCCGAGC TGCAGCTGAA AGAGAAAGCA GTGCCCATCC CGGAGGCTAG CTCCTTCTTC 
ATCTTCAGTC CCACCAACAA GATCCGTGTC CTGTGTCACC GCATTGTCAA TGCCACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCCTGGC CGCTGAGGAT 
CCCATCCGGG CCGACTCCAT GAGGAATCAG ATCCTCGAGT ATTTTGATTA TGTGTTCACT 
GCTGTTTTCA CGGTGGAGAT TGTCCTTAAG ATGACCACCT ATGGAGCCTT CCTGCACAAA 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC TGTGTCCCTC 
ATCTCCATGG GCCTCGAGTC CAGCGCCATC TCCGTGGTGA AGATCTTAAG GGTTCTGAGA 
GTGCTGAGAC CACTCCGGGC CATAAACAGA GCCAAAGGGT TGAAGCACGT GGTGCAGTGC 
GTGTTTGTTG CCATTCGCAC CATCGGGAAC ATCGTGCTGG TCACCACGCT CCTGCAGTTC 
ATGTTTGCGT GCATTGGAGT GCAGCTTTTC AAGGGAAAGT TCTACAGCTG CAATGACTTA 
TCCAAGATGA CTGAAGAGGA ATGCAGGGGC TACTACTACA TATACAAGGA TGGAGACCCC 
ACTCAGATAG AGCTGCGTCC CCGCCAGTGG ATACACAATG ACTTCCACTT TGACAATGTA 
CTCTCCGCTA TGATGTCACT CTTCACGGTC TCCACCTTCG AGGGTTGGCC TCAGCTACTG 
TACAAGGCCA TAGACTCAAA TGAGGAGGAC ACCGGCCCTG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATTAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC CTTCCAGGAA CAGGGAGAGA CTGAGTATAA GAACTGTGAG 
CTCGACAAGA ACCAGCGCCA ATGCGTACAG TATGCCCTGA AGGCCCGCCC ACTGAGGTGT 
TACATCCCCA AAAACCCATA CCAGTATCAG GTGTGGTATG TCGTCACTTC CTCCTACTTT 
GAATACCTGA TGTTTGCTCT CATCATGCTC AATACTATCT GCCTGGGCAT GCAGCATTAC 
AACCAGTCGG AACAGATGAA CCACATCTCA GACATTCTCA ACGTGGCTTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAGCTCATA GCTTTCAAGC CCAGGGGCTA TTTTGGAGAT 
CCTTGGAATG TGTTTGACTT CCTAATCGTC ATCGGCAGCA TCATTGACGT CATCCTGAGC 
GAGATCGATA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGATGAGAG CGCCCGTATC TCCAGTGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGACTGG TCAAGCTGCT GAACCGGGCG GAGGGTGTGC GCACCCTGCT GTGGACATTC 
ATCAAGTCCT TCCAGGCCCT GCCGTACGTG GCTTTGCTGA TCGTGATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCA TGGTGGACGG GACGCAGATA 
AACCGGAACA ACAACTTCCA GACTTTCCCA CAGGCCGTGC TGCTGCTCTT CAGGTGTGCC 
ACAGGGGAAG CCTGGCAAGA GATCCTGCTG GCCTGCAGCT ATGGGAAGCT GTGTGACCCG 
GAGTCTGACT ACGCACCCGG GGAGGAGCAT ACATGCGGCA CCAACTTCGC CTACTACTAC 
TTCATCAGTT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCTGTCATC 
ATGGACAACT TTGATTACCT CACAAGGGAC TGGTCCATTC TGGGCCCTCA CCACCTGGAC 
GAGTTCAAGG CCATTTGGGC TGAGTACGAC CCGGAGGCTA AGGGCAGGAT CAAGCACCTG 
GATGTGGTGA CCCTGCTGAG GAGGATTCAG CCCCCTCTGG GCTTTGGGAA GTTCTGCCCA 
CACAGGGTAG CATGTAAGAG GCTGGTGGGC ATGAACATGC CCCTGAACAG CGATGGCACA 
GTCACCTTTA ATGCCACACT ATTTGCCCTG GTGCGCACAG CCCTCAAGAT CAAGACAGAA 
GGCAACTTTG AACAAGCTAA CGAGGAGCTG AGAGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCTTGGA CCAAGTCATC CCTCCAATAG GAGATGATGA GGTGACAGTA 
GGGAAATTCT ATGCCACATT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAGGAATATT ATGGGTATCG ACCCAAAAAG GACACAGTTC AGATACAGGC TGGATTACGG 
ACCATAGAGG AAGAGGCAGC TCCTGAGATC CACCGTGCCA TCTCTGGAGA CCTGACAGCA 
GAGGAGGAAC TGGAGAGGGC CATGGTAGAG GCTGCCATGG AGGAGGGGAT ATTCCGGAGG 
ACGGGAGGCC TGTTTGGTCA AGTGGACAAC TTCCTGGAAA GGACCAACTC TCTGCCCCCT 
GTCATGGCCA ACCAAAGACC CCTCCAATTT GCTGAAATAG AGATGGAAGA GCTGGAGTCA 
CCAGTCTTCT TGGAGGACTT CCCTCAGAAC CCAGGCACCC ACCCTCTTGC TCGTGCCAAT 
ACCAACAACG CCAATGCCAA TGTTGCCTAT GGCAACAGCA GCCATAGAAA CAACCCAGTG 
TTTTCCAGCA TCTGCTATGA ACGGGAGTTC TTGGGAGAGG CGGACATGCC TGTTACCAGA 
GAAGGACCCC TCAGTCAGCC CTGCAGGGCC TCAGGACCTC ACAGCAGATC TCATGTGGAC 
AAACTGAAAA GACCGATGAC CCAGAGAGGA ATGCCTGAAG GCCAGGTGCC TCCTTCCCCG 
TGCCAGCTCT CCCAGGCAGA GCATCCTGTG CAGAAAGAGG GAAAGGGCCC CACGTCACGG 
TTTCTGGAAA CACCCAACAG CAGGAACTTT GAGGAGCACG TTCCCAGGAA TTCGGCTCAC 
AGGTGCACGG CTCCAGCTAC TGCCATGCTG ATCCAGGAGG CTCTGGTTCG AGGAGGACTA 
GACTCCTTAG CAGCAGATGC TAACTTCGTC ATGGCAACAG GCCAGGCCCT GGCAGATGCC 
TGCCAGATGG AACCAGAGGA AGTCGAGGTG GCAGCAACAG AGCTACTGAA GCAAGAATCC 
CCAGAGGGCG GGGCCGTGCC CTGGGAGCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Common cellular origin and diverging developmental programs for different sesamoid bones.
Development (Cambridge, England)146(4)(2019 02)
Eyal S,Rubin S,Krief S,Levin L,Zelzer E

STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor.
Journal of cellular physiology233(12)9045-9051(2018 12)
Campiglio M,Kaplan MM,Flucher BE

Fibromodulin modulates myoblast differentiation by controlling calcium channel.
Biochemical and biophysical research communications503(2)580-585(2018 09)
Lee EJ,Nam JH,Choi I

Calcium Influx and Release Cooperatively Regulate AChR Patterning and Motor Axon Outgrowth during Neuromuscular Junction Formation.
Cell reports23(13)3891-3904(2018 06)
Kaplan MM,Sultana N,Benedetti A,Obermair GJ,Linde NF,Papadopoulos S,Dayal A,Grabner M,Flucher BE

Physical interaction of junctophilin and the Ca
Proceedings of the National Academy of Sciences of the United States of America115(17)4507-4512(2018 04)
Nakada T,Kashihara T,Komatsu M,Kojima K,Takeshita T,Yamada M

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Cacna1s cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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