CACNA1SA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1SA
Entrez Gene ID	570683
Full Name	calcium channel, voltage-dependent, L type, alpha 1S subunit, a
General protein information	Preferred Names calcium channel, voltage-dependent, L type, alpha 1S subunit, a Names calcium channel, voltage-dependent, L type, alpha 1S subunit, a cacna1s cav1.1a1sa voltage dependent calcium channel alpha-1S subunit
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001146150.1	NP_001139622.1	calcium channel, voltage-dependent, L type, alpha 1S subunit, a
XM_005168275.4	XP_005168332.1	calcium channel, voltage-dependent, L type, alpha 1S subunit, a isoform X1
XM_005168275.3	XP_005168332.1	calcium channel, voltage-dependent, L type, alpha 1S subunit, a isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CACNA1S	XP_002802046.1
Rattus norvegicus (Norway rat)	Cacna1s	NP_446325.1
Homo sapiens (human)	CACNA1S	NP_000060.2
Pan troglodytes (chimpanzee)	CACNA1S	XP_525018.3
Canis lupus familiaris (dog)	CACNA1S	XP_848685.3
Mus musculus (house mouse)	Cacna1s	NP_001074492.1
Bos taurus (cattle)	CACNA1S	XP_002694288.2
Danio rerio (zebrafish)	cacna1sa	NP_001139622.1
Gallus gallus (chicken)	CACNA1S	XP_419259.3
Danio rerio (zebrafish)	cacna1sb	NP_999891.1

Gene Ontology
Bibliography

Related articles in PubMed

Ca
Dayal A, Ng SFJ, Grabner M
Nature communications10(1)115(2019 01)

Bifenthrin causes transcriptomic alterations in mTOR and ryanodine receptor-dependent signaling and delayed hyperactivity in developing zebrafish (Danio rerio).
Frank DF, Miller GW, Harvey DJ, Brander SM, Geist J, Connon RE, Lein PJ
Aquatic toxicology (Amsterdam, Netherlands)20050-61(2018 Jul)

Expression of calcium channel transcripts in the zebrafish heart: dominance of T-type channels.
Haverinen J, Hassinen M, Dash SN, Vornanen M
The Journal of experimental biology221(Pt 10)(2018 05)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
Frank DF, Miller GW, Connon RE, Geist J, Lein PJ
PeerJ5e4106(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CACNA1SA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1SA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35778	NM_001146150.1 Latest version!	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa26325	XM_005168275.4 Latest version!	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa26325	XM_005168275.3	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1SA ( NM_001146150.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001139622.1 Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), mRNA.

Price & Availability↑

CloneID	ODa35778
Clone ID Related Accession (Same CDS sequence)	NM_001146150.1
Accession Version	NM_001146150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, L type, alpha 1S subunit, a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ769223.1. On Apr 1, 2009 this sequence version replaced XM_694190.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ769223.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGAAAACAGC GTGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCTGCA 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGAGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGTCGAG GTGATTGGCC CGGACCAAAT 
AACGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTAAATG ACGCAATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGATGTGAAC CACATTTCCC AGCATGCATC AGTGCATCCC GACTGCATAG TCCAGCGAGC 
GCAGCCGAGT TCCTCATCCA ACAGGTTCTG ACCTCTGGAG GTCTGGAGAG TCTGGCCAGT 
GACTGGCGCT TTGTGTCCGT GACAAAGTCT GAGATGGCAG CGGCCCTTCA TCTGGATCTG 
CAAGAGCTGG AGAGAAAATC AGCAGCCATA CTCAATCAAC ATAAAGGACA ATGTGTGCTC 
AGAAAGAAGA AGATGGAGAC CTCTCATTCA GACATTACAC ACTGCCATGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139622.1
CDS	1..5334
Translation	MEGNGEAPMSSFIMDEETLKRKQREKLKKLQATGGNPRPARSLLFLTLKNPFRKACINIVEWKPFEIIILLTIFANCVALAVFMPMPEEDTNNTNSNLESLEYIFLIIFTMECFLKIVAYGFLFHADAYLRNCWNILDFVIVTMGLFTVVVDFINSISGVEAPVEQKGGFDMKALRAFRVLRPLRLVSGVPSLQVVMSSILKSMLPLFHISLLVFFMVTIYAIIGLELFKCKMHKTCYHTGTDIIATGDDAQAAPCAQAGNGRRCTLNGTECRGDWPGPNNGITHFDNLGFSMLTVYQCITTQGWTDVLYWVNDAIGMEWPWLYFVTLILLGSFFILNLVLGVLCGEFTKEREKSSRSGEYQILRERQQFDEDLKGYMEWITQAEVMDNDQEGQGLLPLQDGSETETLYELDILNKLMFYVRHARRWNRFFRRKCRVWVKSKLFYWLVILLVFFNTLAIATEHHQQPDSLTNFQDNTNKALLSLFAVEMFLKMYAMGLPSYFMSLFNRFDCFVVSVGILELILVRMDVMSVMGISVLRCIRLLRIIKITRHWTTLSNLVASLLNSVRSIASLLLLLFLFIVIFALLGMQVFGGKFNFPDDRVRRSNFDNFPQALITVFQILTGEGWNYVMYDGIMAHGGPAIPGILVSIYFIILFICGNYILLNVFLAIAVDNLAEAESLTSAQKEKAEEKKRKRLLRENLPDKGEEEKALLAKKLAEQRAKIDGIPTTAKLKVDEFESNVNEIKDPFPPADFPGDDEEEEPEIPLSPRPRPMADLQLKETEVPMPEASAFFLFGPQNKFRKLCHRIINATTFTNIILLFILLSSISLAAEDPIDPMSFRNQVLAYADYVFTSVFTAEIVLKMTTYGAFLHKGSFCRNSFNILDLIVVGVSLLSMGMESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTMLLDFMFACIGVQLFKGKFLYCTDPLKMTAEECQGTFIQHQENALHDMVVSQRLWMNSDLNFDNVLNGMLALFTVSTFEGWPDLLYKAIDSNLENMGPVYNNHIEISIFFIVYLILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSCYFEYLMFLLIMLNTMCLGMQHCKQSDHITDLADTLNVIFTVLFTVEMILKLGAFKAKGYFGDPWNVFDFVIVVGSIVDVILSEIDAALAAQGGLYCLTGCSEVNPMQAIADSENMSVSITLFRLFRVMRLVKLLNRFEGIRNLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALLDGTIINRNNNFQTFPQAVLLLFRCATGEGWHEIMLGCLYGQRCDPKSEYLPGEEYTCGSGFAILYFMSFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPQHLDEFKKIWAEYDPEATGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLISMNMPLNSDGTVTFNATLFALVRTGLKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPALGDDEVTVGKFYATFLIQDHFRKFMKRQEEYYGYRPSKKNKNTTEIQAGLRSIEEEAAPELQRAISGDLLNDEEMDRAMEESGEESIYRRAGGLFGNHVDPFSMERGTGAAQVTSQRPLQLADSRADRTETTYASYHANNNNTNNNNRRRHRCEPHFPACISASRLHSPASAAEFLIQQVLTSGGLESLASDWRFVSVTKSEMAAALHLDLQELERKSAAILNQHKGQCVLRKKKMETSHSDITHCHV

Target ORF information:

RefSeq Version	NM_001146150.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146150.1

ORF Insert Sequence:

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGAAAACAGC GTGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCTGCA 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGAGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGTCGAG GTGATTGGCC CGGACCAAAT 
AACGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTAAATG ACGCAATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGATGTGAAC CACATTTCCC AGCATGCATC AGTGCATCCC GACTGCATAG TCCAGCGAGC 
GCAGCCGAGT TCCTCATCCA ACAGGTTCTG ACCTCTGGAG GTCTGGAGAG TCTGGCCAGT 
GACTGGCGCT TTGTGTCCGT GACAAAGTCT GAGATGGCAG CGGCCCTTCA TCTGGATCTG 
CAAGAGCTGG AGAGAAAATC AGCAGCCATA CTCAATCAAC ATAAAGGACA ATGTGTGCTC 
AGAAAGAAGA AGATGGAGAC CTCTCATTCA GACATTACAC ACTGCCATGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1SA ( XM_005168275.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168332.1 Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.

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CloneID	ODa26325
Clone ID Related Accession (Same CDS sequence)	XM_005168275.4 , XM_005168275.3
Accession Version	XM_005168275.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, L type, alpha 1S subunit, a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168275.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGGAAACAGC GGGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCCGCG 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGGGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGCCGAG GTGATTGGCC CGGACCAAAT 
AATGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTGAATG ACGCGATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGTAGATGTG AACCACATTT CCCAGCATGC ATCAGTGCAT CCCGACTGCA TAGTCCAGCG 
AGCGCAGCCG AGTTCCTCAT CCAACAGGTT CTGACCTCTG GAGGTCTGGA GAGTCTGGCC 
AGTGACTGGC GCTTTGTGTC CGTGACAAAG TCTGAGATGG CAGCGGCCCT TCATCTGGAT 
CTGCAAGAGC TGGAGAGAAA ATCAGCAGCC ATACTCAATC AACATAAAGG ACAATGTGTG 
CTCAGAAAGA AGAAGATGGA GACCTCTCAT TCAGACATTA CACACTGCCA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168332.1
CDS	383..5719
Translation	MEGNGEAPMSSFIMDEETLKRKQREKLKKLQATGGNPRPARSLLFLTLKNPFRKACINIVEWKPFEIIILLTIFANCVALAVFMPMPEEDTNNTNSNLESLEYIFLIIFTMECFLKIVAYGFLFHADAYLRNCWNILDFVIVTMGLFTVVVDFINSISGVEAPVEQKGGFDMKALRAFRVLRPLRLVSGVPSLQVVMSSILKSMLPLFHISLLVFFMVTIYAIIGLELFKCKMHKTCYHTGTDIIATGDDAQAAPCAQAGNGRRCTLNGTECRGDWPGPNNGITHFDNLGFSMLTVYQCITTQGWTDVLYWVNDAIGMEWPWLYFVTLILLGSFFILNLVLGVLCGEFTKEREKSSRSGEYQILRERQQFDEDLKGYMEWITQAEVMDNDQEGQGLLPLQDGSETETLYELDILNKLMFYVRHARRWNRFFRRKCRVWVKSKLFYWLVILLVFFNTLAIATEHHQQPDSLTNFQDNTNKALLSLFAVEMFLKMYAMGLPSYFMSLFNRFDCFVVSVGILELILVRMDVMSVMGISVLRCIRLLRIIKITRHWTTLSNLVASLLNSVRSIASLLLLLFLFIVIFALLGMQVFGGKFNFPDDRVRRSNFDNFPQALITVFQILTGEGWNYVMYDGIMAHGGPAIPGILVSIYFIILFICGNYILLNVFLAIAVDNLAEAESLTSAQKEKAEEKKRKRLLRENLPDKGEEEKALLAKKLAEQRAKIDGIPTTAKLKVDEFESNVNEIKDPFPPADFPGDDEEEEPEIPLSPRPRPMADLQLKETEVPMPEASAFFLFGPQNKFRKLCHRIINATTFTNIILLFILLSSISLAAEDPIDPMSFRNQVLAYADYVFTSVFTAEIVLKMTTYGAFLHKGSFCRNSFNILDLIVVGVSLLSMGMESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTMLLDFMFACIGVQLFKGKFLYCTDPLKMTAEECQGTFIQHQENALHDMVVSQRLWMNSDLNFDNVLNGMLALFTVSTFEGWPDLLYKAIDSNLENMGPVYNNHIEISIFFIVYLILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSCYFEYLMFLLIMLNTMCLGMQHCKQSDHITDLADTLNVIFTVLFTVEMILKLGAFKAKGYFGDPWNVFDFVIVVGSIVDVILSEIDAALAAQGGLYCLTGCSEVNPMQAIADSENMSVSITLFRLFRVMRLVKLLNRFEGIRNLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALLDGTIINRNNNFQTFPQAVLLLFRCATGEGWHEIMLGCLYGQRCDPKSEYLPGEEYTCGSGFAILYFMSFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPQHLDEFKKIWAEYDPEATGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLISMNMPLNSDGTVTFNATLFALVRTGLKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPALGDDEVTVGKFYATFLIQDHFRKFMKRQEEYYGYRPSKKNKNTTEIQAGLRSIEEEAAPELQRAISGDLLNDEEMDRAMEESGEESIYRRAGGLFGNHVDPFSMERGTGAAQVTSQRPLQLADSRADRTETTYASYHANNNNTNNNNRRRHSRCEPHFPACISASRLHSPASAAEFLIQQVLTSGGLESLASDWRFVSVTKSEMAAALHLDLQELERKSAAILNQHKGQCVLRKKKMETSHSDITHCHV

Target ORF information:

RefSeq Version	XM_005168275.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168275.4

ORF Insert Sequence:

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGGAAACAGC GGGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCCGCG 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGGGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGCCGAG GTGATTGGCC CGGACCAAAT 
AATGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTGAATG ACGCGATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGTAGATGTG AACCACATTT CCCAGCATGC ATCAGTGCAT CCCGACTGCA TAGTCCAGCG 
AGCGCAGCCG AGTTCCTCAT CCAACAGGTT CTGACCTCTG GAGGTCTGGA GAGTCTGGCC 
AGTGACTGGC GCTTTGTGTC CGTGACAAAG TCTGAGATGG CAGCGGCCCT TCATCTGGAT 
CTGCAAGAGC TGGAGAGAAA ATCAGCAGCC ATACTCAATC AACATAAAGG ACAATGTGTG 
CTCAGAAAGA AGAAGATGGA GACCTCTCAT TCAGACATTA CACACTGCCA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1SA ( XM_005168275.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168332.1 Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26325
Clone ID Related Accession (Same CDS sequence)	XM_005168275.4 , XM_005168275.3
Accession Version	XM_005168275.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	calcium channel, voltage-dependent, L type, alpha 1S subunit, a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168275.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGGAAACAGC GGGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCCGCG 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGGGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGCCGAG GTGATTGGCC CGGACCAAAT 
AATGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTGAATG ACGCGATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGTAGATGTG AACCACATTT CCCAGCATGC ATCAGTGCAT CCCGACTGCA TAGTCCAGCG 
AGCGCAGCCG AGTTCCTCAT CCAACAGGTT CTGACCTCTG GAGGTCTGGA GAGTCTGGCC 
AGTGACTGGC GCTTTGTGTC CGTGACAAAG TCTGAGATGG CAGCGGCCCT TCATCTGGAT 
CTGCAAGAGC TGGAGAGAAA ATCAGCAGCC ATACTCAATC AACATAAAGG ACAATGTGTG 
CTCAGAAAGA AGAAGATGGA GACCTCTCAT TCAGACATTA CACACTGCCA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168332.1
CDS	371..5707
Translation	MEGNGEAPMSSFIMDEETLKRKQREKLKKLQATGGNPRPARSLLFLTLKNPFRKACINIVEWKPFEIIILLTIFANCVALAVFMPMPEEDTNNTNSNLESLEYIFLIIFTMECFLKIVAYGFLFHADAYLRNCWNILDFVIVTMGLFTVVVDFINSISGVEAPVEQKGGFDMKALRAFRVLRPLRLVSGVPSLQVVMSSILKSMLPLFHISLLVFFMVTIYAIIGLELFKCKMHKTCYHTGTDIIATGDDAQAAPCAQAGNGRRCTLNGTECRGDWPGPNNGITHFDNLGFSMLTVYQCITTQGWTDVLYWVNDAIGMEWPWLYFVTLILLGSFFILNLVLGVLCGEFTKEREKSSRSGEYQILRERQQFDEDLKGYMEWITQAEVMDNDQEGQGLLPLQDGSETETLYELDILNKLMFYVRHARRWNRFFRRKCRVWVKSKLFYWLVILLVFFNTLAIATEHHQQPDSLTNFQDNTNKALLSLFAVEMFLKMYAMGLPSYFMSLFNRFDCFVVSVGILELILVRMDVMSVMGISVLRCIRLLRIIKITRHWTTLSNLVASLLNSVRSIASLLLLLFLFIVIFALLGMQVFGGKFNFPDDRVRRSNFDNFPQALITVFQILTGEGWNYVMYDGIMAHGGPAIPGILVSIYFIILFICGNYILLNVFLAIAVDNLAEAESLTSAQKEKAEEKKRKRLLRENLPDKGEEEKALLAKKLAEQRAKIDGIPTTAKLKVDEFESNVNEIKDPFPPADFPGDDEEEEPEIPLSPRPRPMADLQLKETEVPMPEASAFFLFGPQNKFRKLCHRIINATTFTNIILLFILLSSISLAAEDPIDPMSFRNQVLAYADYVFTSVFTAEIVLKMTTYGAFLHKGSFCRNSFNILDLIVVGVSLLSMGMESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTMLLDFMFACIGVQLFKGKFLYCTDPLKMTAEECQGTFIQHQENALHDMVVSQRLWMNSDLNFDNVLNGMLALFTVSTFEGWPDLLYKAIDSNLENMGPVYNNHIEISIFFIVYLILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSCYFEYLMFLLIMLNTMCLGMQHCKQSDHITDLADTLNVIFTVLFTVEMILKLGAFKAKGYFGDPWNVFDFVIVVGSIVDVILSEIDAALAAQGGLYCLTGCSEVNPMQAIADSENMSVSITLFRLFRVMRLVKLLNRFEGIRNLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALLDGTIINRNNNFQTFPQAVLLLFRCATGEGWHEIMLGCLYGQRCDPKSEYLPGEEYTCGSGFAILYFMSFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPQHLDEFKKIWAEYDPEATGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLISMNMPLNSDGTVTFNATLFALVRTGLKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPALGDDEVTVGKFYATFLIQDHFRKFMKRQEEYYGYRPSKKNKNTTEIQAGLRSIEEEAAPELQRAISGDLLNDEEMDRAMEESGEESIYRRAGGLFGNHVDPFSMERGTGAAQVTSQRPLQLADSRADRTETTYASYHANNNNTNNNNRRRHSRCEPHFPACISASRLHSPASAAEFLIQQVLTSGGLESLASDWRFVSVTKSEMAAALHLDLQELERKSAAILNQHKGQCVLRKKKMETSHSDITHCHV

Target ORF information:

RefSeq Version	XM_005168275.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, L type, alpha 1S subunit, a (cacna1sa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168275.3

ORF Insert Sequence:

ATGGAGGGAA ACGGCGAAGC TCCCATGAGC TCCTTCATCA TGGATGAGGA GACGCTGAAG 
AGGAAACAGC GGGAAAAGCT GAAGAAGCTC CAGGCCACCG GGGGGAACCC TCGTCCCGCG 
CGCTCACTGC TGTTCCTCAC ACTCAAAAAC CCATTTCGCA AGGCCTGCAT CAACATCGTG 
GAGTGGAAAC CGTTTGAGAT CATCATCCTC CTCACCATCT TTGCTAATTG TGTAGCGCTG 
GCCGTTTTCA TGCCCATGCC CGAGGAAGAC ACAAATAACA CCAACAGCAA CCTAGAGAGT 
CTGGAGTACA TTTTTCTGAT CATCTTCACG ATGGAGTGCT TCCTGAAGAT CGTGGCGTAC 
GGTTTCCTCT TTCACGCTGA TGCTTATTTA CGAAACTGCT GGAATATTCT AGATTTCGTC 
ATCGTGACCA TGGGGCTGTT CACAGTGGTG GTGGACTTCA TCAACAGCAT CAGCGGTGTG 
GAGGCGCCGG TGGAGCAGAA GGGGGGATTC GACATGAAGG CGCTGCGAGC GTTCAGAGTG 
CTGCGGCCCC TGCGCCTCGT TTCCGGGGTC CCCAGCCTGC AGGTGGTGAT GAGCTCCATC 
CTCAAGTCCA TGCTGCCTCT GTTCCACATC TCCCTGCTGG TTTTCTTCAT GGTCACCATC 
TACGCTATCA TCGGCCTGGA GCTTTTTAAA TGCAAGATGC ATAAAACATG CTACCACACC 
GGCACAGATA TTATTGCCAC AGGGGACGAT GCTCAGGCAG CTCCCTGTGC TCAGGCCGGT 
AATGGCAGGC GATGCACTCT GAACGGAACT GAATGCCGAG GTGATTGGCC CGGACCAAAT 
AATGGCATCA CTCACTTTGA CAATCTGGGT TTCTCCATGC TCACGGTTTA CCAGTGCATC 
ACCACTCAGG GCTGGACGGA CGTTCTGTAC TGGGTGAATG ACGCGATTGG GATGGAGTGG 
CCCTGGCTGT ATTTCGTGAC TCTGATCCTC CTGGGCTCGT TCTTCATCTT GAATCTGGTT 
CTGGGTGTTT TGTGTGGAGA ATTCACCAAG GAGCGAGAGA AGAGTTCTCG AAGTGGAGAG 
TATCAAATAC TACGGGAGCG ACAGCAGTTC GATGAGGATC TGAAGGGATA CATGGAGTGG 
ATCACACAGG CGGAAGTCAT GGACAATGAC CAGGAGGGGC AAGGTCTACT CCCCCTACAG 
GACGGATCTG AAACAGAGAC CCTCTATGAG CTGGATATAT TGAACAAACT CATGTTTTAT 
GTTCGGCATG CTCGTCGCTG GAACCGTTTC TTCAGGAGGA AGTGTCGTGT GTGGGTGAAG 
TCTAAGCTGT TCTATTGGCT GGTGATTCTG CTGGTGTTTT TCAACACTCT GGCCATCGCA 
ACCGAACACC ATCAGCAGCC CGACTCGCTC ACCAACTTCC AAGACAATAC CAATAAGGCG 
CTGTTGTCTC TGTTTGCGGT GGAGATGTTC CTGAAGATGT ACGCGATGGG GCTGCCGTCA 
TACTTCATGT CACTCTTCAA CCGTTTCGAC TGCTTCGTGG TGTCGGTGGG CATCCTGGAG 
CTCATCCTCG TACGCATGGA CGTCATGTCT GTGATGGGGA TCTCCGTTCT GCGCTGCATT 
CGGCTTCTGC GGATCATCAA AATAACCCGC CACTGGACAA CTCTCAGTAA TCTGGTAGCA 
TCGCTCCTGA ACTCAGTGCG CTCTATCGCC TCCCTGCTGC TCCTCCTCTT CCTCTTCATA 
GTCATCTTCG CTCTGCTCGG GATGCAGGTT TTTGGCGGAA AGTTCAATTT TCCAGATGAC 
CGCGTGAGAC GAAGCAACTT CGACAACTTC CCTCAAGCTC TGATAACAGT TTTCCAGATC 
CTGACAGGCG AAGGCTGGAA CTATGTCATG TACGACGGCA TCATGGCTCA CGGAGGTCCT 
GCCATACCTG GAATCCTGGT CAGCATTTAC TTCATCATTC TGTTCATCTG TGGGAACTAC 
ATCCTCCTGA ATGTGTTTTT AGCAATAGCG GTGGATAATC TGGCCGAGGC GGAGAGTCTG 
ACCTCTGCGC AGAAGGAAAA AGCAGAGGAG AAGAAGCGGA AAAGGCTTCT TAGAGAAAAT 
CTGCCAGACA AGGGCGAGGA GGAAAAGGCC CTGCTAGCAA AGAAACTAGC GGAGCAGAGA 
GCCAAGATTG ATGGAATTCC CACCACAGCC AAGCTAAAAG TGGACGAATT TGAATCGAAT 
GTGAACGAGA TCAAAGATCC GTTTCCTCCT GCTGATTTCC CAGGTGATGA TGAGGAGGAG 
GAGCCAGAGA TCCCGCTTAG TCCTCGGCCT CGACCAATGG CAGACCTGCA GCTGAAGGAG 
ACAGAGGTGC CCATGCCGGA GGCCAGCGCT TTCTTCCTCT TTGGCCCGCA GAACAAGTTC 
CGCAAACTCT GCCATCGCAT CATAAACGCC ACGACCTTCA CCAACATCAT CCTCCTCTTC 
ATCCTGCTCA GCAGTATCTC TCTGGCTGCG GAGGATCCCA TCGACCCCAT GTCCTTCAGG 
AACCAGGTCC TGGCCTACGC TGATTACGTC TTCACTTCAG TCTTTACTGC AGAGATTGTG 
CTGAAGATGA CAACGTACGG TGCGTTCCTA CACAAGGGTT CCTTCTGCCG AAACTCCTTC 
AACATCCTGG ACCTGATTGT GGTCGGCGTC TCGCTGCTGT CAATGGGAAT GGAATCAAGT 
GCCATCTCAG TAGTGAAGAT CTTGAGGGTC TTGAGAGTAT TGAGGCCTTT AAGGGCTATC 
AATCGAGCCA AAGGCCTCAA ACATGTGGTC CAGTGTGTGT TTGTGGCCAT CAAGACCATC 
GGCAACATCG TTTTAGTCAC CATGCTCCTG GATTTCATGT TCGCCTGCAT CGGCGTCCAA 
CTTTTCAAGG GGAAATTCCT ATACTGCACA GACCCGCTGA AAATGACGGC AGAGGAATGC 
CAGGGAACGT TTATTCAACA TCAGGAGAAC GCTCTTCATG ATATGGTGGT CAGTCAACGC 
TTGTGGATGA ACAGCGACCT GAACTTCGAC AACGTTCTGA ACGGCATGCT GGCTCTCTTC 
ACCGTGTCCA CATTCGAGGG ATGGCCAGAC TTGCTCTACA AAGCCATCGA CTCGAATCTG 
GAAAACATGG GCCCTGTTTA CAACAACCAC ATCGAGATCT CCATCTTCTT CATCGTCTAC 
CTGATCCTCA TCGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTCGTCAT CGTCACCTTC 
CAGGAACAGG GCGAGCAGGA GTACAAGAAC TGCGAACTGG ACAAAAACCA GCGTCAGTGT 
GTCCAGTACG CCCTCAAAGC TCGACCCCTG AGGTGCTACA TCCCCAAAAA CCCCTACCAG 
TACCAGGTCT GGTACATCGT CACCTCCTGC TACTTTGAGT ACCTGATGTT CCTCCTCATC 
ATGCTCAACA CCATGTGCCT CGGGATGCAG CACTGTAAGC AGTCTGATCA CATCACTGAT 
CTGGCAGACA CGCTGAACGT CATCTTCACC GTCCTGTTCA CAGTGGAGAT GATCCTCAAA 
CTTGGCGCAT TTAAAGCAAA GGGATACTTC GGAGACCCCT GGAACGTCTT TGATTTTGTG 
ATTGTGGTCG GCAGTATTGT TGACGTAATA CTCAGTGAAA TTGACGCGGC TCTCGCGGCT 
CAGGGCGGAC TCTACTGCCT CACCGGCTGC TCTGAAGTGA ATCCAATGCA GGCTATAGCA 
GACTCGGAGA ACATGAGTGT TTCCATCACA CTCTTCCGGC TCTTCCGCGT CATGCGTTTG 
GTGAAACTCC TCAACCGTTT TGAAGGCATT CGTAACCTGC TGTGGACCTT CATCAAATCA 
TTCCAGGCTT TGCCATACGT GGCTCTCCTC ATCGTTATGC TCTTCTTTAT ATACGCCGTC 
ATTGGGATGC AGGTGTTTGG CAAAATTGCT TTACTAGATG GCACCATAAT CAACCGTAAC 
AACAACTTCC AGACATTTCC TCAAGCCGTT TTACTGCTGT TCCGGTGTGC CACAGGTGAA 
GGCTGGCATG AGATCATGTT GGGTTGTTTA TACGGTCAGC GATGCGACCC AAAGTCAGAA 
TATCTGCCCG GAGAAGAGTA CACGTGTGGA TCTGGATTCG CCATCCTCTA CTTCATGAGC 
TTTTACATGC TCTGTGCTTT TCTAATCATA AACTTGTTTG TCGCTGTGAT CATGGACAAC 
TTCGACTATC TGACCCGCGA TTGGTCGATT CTGGGTCCGC AACATTTAGA CGAGTTTAAG 
AAGATCTGGG CTGAATACGA CCCCGAGGCC ACGGGCCGCA TAAAACACCT GGACGTGGTG 
ACGCTGCTGA GGAGAATACA GCCTCCGCTC GGATTCGGAA AATTCTGTCC TCATCGGGTC 
GCCTGCAAGA GACTGATTTC CATGAATATG CCCTTGAACA GCGACGGAAC CGTAACGTTT 
AACGCCACAC TCTTCGCTCT GGTCAGAACG GGACTGAAGA TTAAAACTGA AGGTAATTTC 
GAGCAGGCGA ATGAAGAGCT GCGTGCAATT ATTAAGAAGA TCTGGAAACG CACCAGTATG 
AAACTGCTCG ACCAGGTCAT TCCAGCACTC GGAGATGACG AGGTGACAGT GGGCAAGTTC 
TACGCCACGT TTCTGATCCA GGACCACTTC CGGAAGTTCA TGAAGAGACA GGAGGAATAT 
TACGGATACC GACCGAGCAA GAAGAATAAA AACACCACAG AGATCCAGGC GGGTCTGAGG 
AGCATCGAGG AGGAGGCGGC TCCTGAACTC CAGAGAGCCA TTTCTGGAGA CCTCCTTAAT 
GACGAGGAGA TGGACAGAGC GATGGAGGAG TCCGGAGAGG AGAGCATCTA TAGGCGTGCT 
GGAGGTCTGT TTGGAAACCA TGTGGATCCG TTCAGCATGG AGAGAGGAAC CGGGGCAGCA 
CAGGTGACCA GCCAGAGACC CCTACAGCTC GCAGATAGCA GAGCGGACAG GACGGAGACC 
ACATACGCCA GTTACCATGC CAACAACAAC AACACCAATA ACAACAACAG ACGCAGACAC 
AGTAGATGTG AACCACATTT CCCAGCATGC ATCAGTGCAT CCCGACTGCA TAGTCCAGCG 
AGCGCAGCCG AGTTCCTCAT CCAACAGGTT CTGACCTCTG GAGGTCTGGA GAGTCTGGCC 
AGTGACTGGC GCTTTGTGTC CGTGACAAAG TCTGAGATGG CAGCGGCCCT TCATCTGGAT 
CTGCAAGAGC TGGAGAGAAA ATCAGCAGCC ATACTCAATC AACATAAAGG ACAATGTGTG 
CTCAGAAAGA AGAAGATGGA GACCTCTCAT TCAGACATTA CACACTGCCA TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ca
Nature communications10(1)115(2019 01)
Dayal A,Ng SFJ,Grabner M

Bifenthrin causes transcriptomic alterations in mTOR and ryanodine receptor-dependent signaling and delayed hyperactivity in developing zebrafish (Danio rerio).
Aquatic toxicology (Amsterdam, Netherlands)20050-61(2018 Jul)
Frank DF,Miller GW,Harvey DJ,Brander SM,Geist J,Connon RE,Lein PJ

Expression of calcium channel transcripts in the zebrafish heart: dominance of T-type channels.
The Journal of experimental biology221(Pt 10)(2018 05)
Haverinen J,Hassinen M,Dash SN,Vornanen M

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Transcriptomic profiling of mTOR and ryanodine receptor signaling molecules in developing zebrafish in the absence and presence of PCB 95.
PeerJ5e4106(2017)
Frank DF,Miller GW,Connon RE,Geist J,Lein PJ

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CACNA1SA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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