Ddhd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddhd1
Entrez Gene ID	114874
Full Name	DDHD domain containing 1
Synonyms	Mir5131,PA-PLA1
General protein information	Preferred Names DDHD domain containing 1 Names phospholipase DDHD1 DDHD domain-containing protein 1 phosphatidic acid-preferring phospholipase A1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042719.2	NP_001036184.1	phospholipase DDHD1 isoform 3
NM_001039106.3	NP_001034195.2	phospholipase DDHD1 isoform 1
NM_001284399.1	NP_001271328.1	phospholipase DDHD1 isoform 4
NM_176845.5	NP_789815.3	phospholipase DDHD1 isoform 2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	DDHD1	XP_001083968.1
Gallus gallus (chicken)	DDHD1	XP_421479.4
Homo sapiens (human)	DDHD1	NP_001153619.1
Caenorhabditis elegans (roundworm)	ipla-1	NP_001122623.1
Canis lupus familiaris (dog)	DDHD1	XP_005623464.1
Bos taurus (cattle)	DDHD1	NP_788816.1
Pan troglodytes (chimpanzee)	DDHD1	XP_001160076.1
Mus musculus (house mouse)	Ddhd1	NP_001036184.1
Rattus norvegicus (Norway rat)	Ddhd1	NP_001028238.1
Xenopus tropicalis (tropical clawed frog)	ddhd1	XP_002936953.2
Danio rerio (zebrafish)	LOC562868	XP_005159001.1

Gene Ontology
Bibliography

Related articles in PubMed

The Spastic Paraplegia-Associated Phospholipase DDHD1 Is a Primary Brain Phosphatidylinositol Lipase.
Inloes JM, Jing H, Cravatt BF
Biochemistry57(39)5759-5767(2018 10)

Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.
Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman MA, Yamamoto A, Tani K
The Journal of biological chemistry289(16)11497-511(2014 Apr)

Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage.
Spierings DC, McGoldrick D, Hamilton-Easton AM, Neale G, Murchison EP, Hannon GJ, Green DR, Withoff S
Blood117(20)5340-9(2011 May)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddhd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddhd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00420	NM_001039106.3 Latest version!	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu00995	NM_001042719.2 Latest version!	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu06258	NM_176845.5 Latest version!	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu08620	NM_001284399.1 Latest version!	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddhd1 ( NM_001039106.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034195.2 Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00420
Clone ID Related Accession (Same CDS sequence)	NM_001039106.3
Accession Version	NM_001039106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	phospholipase DDHD1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107711.13, AK048690.1, BC043475.1 and AK036369.1. On Oct 1, 2013 this sequence version replaced NM_001039106.2. Transcript Variant: This variant (1) lacks two in-frame exons in the coding region, compared to variant 3. The encoded isoform (1) is shorter, compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: DQ272482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGCTGTTC ACAGTTTCAA GCTGAGTCGG AACCACGTGG ACTGGCACAG CATGGATGAA 
GTCTATCTCT ACAGTGACGC AACAACATCC AAAATTGCGA GAACCGTTAC CCAAAAACTG 
GGCTTTTCTA AAGCCTCCAG CAGTGGGACC AGACTGCACA GAGGTTATGT AGAAGAAGCC 
ACACTGGAAG ACAAGCCGTC GCAGACTTCT CATATCGTGT TTGTTGTGCA CGGCATTGGA 
CAGAAGATGG ACCAAGGAAG AATCATCAAA AACACAGCCA TGATGAGAGA GGCTGCAAGG 
AAAATGGAAG AAAAGCATTT TTCCAACCAT GCAACACATG TTGAATTTCT GCCTGTTGAA 
TGGCGATCAA AGCTTACTCT TGATGGAGAC ACAGTTGATT CCATCACTCC TGACAAAGTG 
CGAGGCCTGA GGGACATGCT CAACAGCAGC GCGATGGACA TCATGTACTA CACCAGCCCA 
CTCTACAGGG ATGAGCTAGT TAAAGGCCTT CAGCAAGAGC TGAATCGATT ATATTCCCTT 
TTCTGTTCCC GGAATCCAGA CTTTGAAGAG AAAGGGGGTA AAGTCTCAAT AGTGTCCCAT 
TCCTTGGGGT GTGTAATCAC TTATGACATC ATGATGGGCT GGAATCCAGG TGGACTCTAT 
GAGCAGCTGC TGCAGAAGGA GGAAGAGCTG CCCGACGAGC GGTGGATGAG CTATGAAGAG 
CGGCATCTTC TTGACGAGCT CTATATAACA AAGCGACGGC TGAGGGAAAT AGAAGATCGG 
CTGCACGGAT TGAAGGCGCC ATCCATATCA CAAACACCTG CCTTAAAATT TAAGGTTGAA 
AATTTCTTCT GTATGGGATC TCCGCTAGCA GTTTTTTTGG CATTACGCGG CATCCGACCA 
GGAAATTCTG GAAGTCAAGA CCACATTTTA CCTAGAGAGA TCTGTAACCG CTTACTAAAT 
ATTTTTCATC CTACGGACCC AGTGGCCTAC AGATTAGAAC CGTTGATTTT GAAACACTAC 
AGCAACATTT CTCCAGTCCA GATCCACTGG TACAACACTT CAAACCCTCT ACCTTATGAG 
CATATGAAGC CAAATTTTCT CAACCCAGCA AAAGAACCTA CCTCAGTTTC AGACAGTGAG 
AACATAGCAG CTATCCCAAG CCCTGTGACC TCCCCCGTCT TATCTCGGCG CCACTATGGA 
GAATCTATAA CTAACATTGG CAAAGCAAGC ATATTGGGGG CTGCTAGCAT TGGAAAGGGC 
CTTGGAGGAA TGTTGTTCTC AAGATTTGGA CGTTCTTCAG CATCACAGCC GTCTGAGCCA 
TCTAAAGACT CACTGGAAGA TGATAAGAAG CCTTCAGCTT CACCCTCTAC CACCACCGTG 
GCAACGCAGA CCCTGCCGCA CAGTGGCTCC GGCTTCCTCG ACTCCGCATT GGAACTGGAA 
CACAGGATCG ACTTTGAACT CAGAGAAGGC CTTGTGGAGA GCCGCTATTG GTCAGCCGTC 
ACGTCGCACA CTGCCTATTG GTCATCCTTG GATGTCGCTC TTTTTCTTTT AACATTCATG 
TACAAACACG AGCATGATAC CGAGGCCAAG CCCAGCCTAG GTTCACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034195.2
CDS	133..2703
Translation	MNYPGRGAPRSPERNGRGGGAWELGSDAAGVFGGGGCCFEHQPGDSVPLSLLRAEPLHLAPGADDLSHLALDPCLSDETYDFSSAESGSSLRYYSEGESAGGGSSSSPPPPLVAQNSGGGGAAGGGPGDRKRARPGGAAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGAQARAAGRDGDRVCGPSSSFGEEDEEDSACGFCPRAAGPEPEMEELVTIEPVCVRGGLYEVDVTQGECYPVYWNQADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLSCFRGQQMQENFDIEVSKSLDGKDAVHSFKLSRNHVDWHSMDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTSHIVFVVHGIGQKMDQGRIIKNTAMMREAARKMEEKHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMMGWNPGGLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLREIEDRLHGLKAPSISQTPALKFKVENFFCMGSPLAVFLALRGIRPGNSGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPNFLNPAKEPTSVSDSENIAAIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSASQPSEPSKDSLEDDKKPSASPSTTTVATQTLPHSGSGFLDSALELEHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDTEAKPSLGSL

Target ORF information:

RefSeq Version	NM_001039106.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039106.3

ORF Insert Sequence:

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGCTGTTC ACAGTTTCAA GCTGAGTCGG AACCACGTGG ACTGGCACAG CATGGATGAA 
GTCTATCTCT ACAGTGACGC AACAACATCC AAAATTGCGA GAACCGTTAC CCAAAAACTG 
GGCTTTTCTA AAGCCTCCAG CAGTGGGACC AGACTGCACA GAGGTTATGT AGAAGAAGCC 
ACACTGGAAG ACAAGCCGTC GCAGACTTCT CATATCGTGT TTGTTGTGCA CGGCATTGGA 
CAGAAGATGG ACCAAGGAAG AATCATCAAA AACACAGCCA TGATGAGAGA GGCTGCAAGG 
AAAATGGAAG AAAAGCATTT TTCCAACCAT GCAACACATG TTGAATTTCT GCCTGTTGAA 
TGGCGATCAA AGCTTACTCT TGATGGAGAC ACAGTTGATT CCATCACTCC TGACAAAGTG 
CGAGGCCTGA GGGACATGCT CAACAGCAGC GCGATGGACA TCATGTACTA CACCAGCCCA 
CTCTACAGGG ATGAGCTAGT TAAAGGCCTT CAGCAAGAGC TGAATCGATT ATATTCCCTT 
TTCTGTTCCC GGAATCCAGA CTTTGAAGAG AAAGGGGGTA AAGTCTCAAT AGTGTCCCAT 
TCCTTGGGGT GTGTAATCAC TTATGACATC ATGATGGGCT GGAATCCAGG TGGACTCTAT 
GAGCAGCTGC TGCAGAAGGA GGAAGAGCTG CCCGACGAGC GGTGGATGAG CTATGAAGAG 
CGGCATCTTC TTGACGAGCT CTATATAACA AAGCGACGGC TGAGGGAAAT AGAAGATCGG 
CTGCACGGAT TGAAGGCGCC ATCCATATCA CAAACACCTG CCTTAAAATT TAAGGTTGAA 
AATTTCTTCT GTATGGGATC TCCGCTAGCA GTTTTTTTGG CATTACGCGG CATCCGACCA 
GGAAATTCTG GAAGTCAAGA CCACATTTTA CCTAGAGAGA TCTGTAACCG CTTACTAAAT 
ATTTTTCATC CTACGGACCC AGTGGCCTAC AGATTAGAAC CGTTGATTTT GAAACACTAC 
AGCAACATTT CTCCAGTCCA GATCCACTGG TACAACACTT CAAACCCTCT ACCTTATGAG 
CATATGAAGC CAAATTTTCT CAACCCAGCA AAAGAACCTA CCTCAGTTTC AGACAGTGAG 
AACATAGCAG CTATCCCAAG CCCTGTGACC TCCCCCGTCT TATCTCGGCG CCACTATGGA 
GAATCTATAA CTAACATTGG CAAAGCAAGC ATATTGGGGG CTGCTAGCAT TGGAAAGGGC 
CTTGGAGGAA TGTTGTTCTC AAGATTTGGA CGTTCTTCAG CATCACAGCC GTCTGAGCCA 
TCTAAAGACT CACTGGAAGA TGATAAGAAG CCTTCAGCTT CACCCTCTAC CACCACCGTG 
GCAACGCAGA CCCTGCCGCA CAGTGGCTCC GGCTTCCTCG ACTCCGCATT GGAACTGGAA 
CACAGGATCG ACTTTGAACT CAGAGAAGGC CTTGTGGAGA GCCGCTATTG GTCAGCCGTC 
ACGTCGCACA CTGCCTATTG GTCATCCTTG GATGTCGCTC TTTTTCTTTT AACATTCATG 
TACAAACACG AGCATGATAC CGAGGCCAAG CCCAGCCTAG GTTCACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddhd1 ( NM_001042719.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001036184.1 Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00995
Clone ID Related Accession (Same CDS sequence)	NM_001042719.2
Accession Version	NM_001042719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Mus musculus(house mouse)
Product	phospholipase DDHD1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107711.13, AK048690.1, BC043475.1 and AK036369.1. On Oct 1, 2013 this sequence version replaced NM_001042719.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). ##Evidence-Data-START## Transcript exon combination :: AK048690.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGTTGTCT ACCACCTCCC TCCCTTCTCC CTCCCTTCTC TGTTCAAATG GAGAGGATAT 
AAGGAGAGCA CAGATGCCAC AGGTCTTAAG CGAAAGCGTT CCCAAGCTGT TCACAGTTTC 
AAGCTGAGTC GGAACCACGT GGACTGGCAC AGCATGGATG AAGTCTATCT CTACAGTGAC 
GCAACAACAT CCAAAATTGC GAGAACCGTT ACCCAAAAAC TGGGCTTTTC TAAAGCCTCC 
AGCAGTGGGA CCAGACTGCA CAGAGGTTAT GTAGAAGAAG CCACACTGGA AGACAAGCCG 
TCGCAGACTT CTCATATCGT GTTTGTTGTG CACGGCATTG GACAGAAGAT GGACCAAGGA 
AGAATCATCA AAAACACAGC CATGATGAGA GAGGCTGCAA GGAAAATGGA AGAAAAGCAT 
TTTTCCAACC ATGCAACACA TGTTGAATTT CTGCCTGTTG AATGGCGATC AAAGCTTACT 
CTTGATGGAG ACACAGTTGA TTCCATCACT CCTGACAAAG TGCGAGGCCT GAGGGACATG 
CTCAACAGCA GCGCGATGGA CATCATGTAC TACACCAGCC CACTCTACAG GGATGAGCTA 
GTTAAAGGCC TTCAGCAAGA GCTGAATCGA TTATATTCCC TTTTCTGTTC CCGGAATCCA 
GACTTTGAAG AGAAAGGGGG TAAAGTCTCA ATAGTGTCCC ATTCCTTGGG GTGTGTAATC 
ACTTATGACA TCATGATGGG CTGGAATCCA GGTGGACTCT ATGAGCAGCT GCTGCAGAAG 
GAGGAAGAGC TGCCCGACGA GCGGTGGATG AGCTATGAAG AGCGGCATCT TCTTGACGAG 
CTCTATATAA CAAAGCGACG GCTGAGGGAA ATAGAAGATC GGCTGCACGG ATTGAAGGCG 
CCATCCATAT CACAAACACC TGCCTTAAAA TTTAAGGTTG AAAATTTCTT CTGTATGGGA 
TCTCCGCTAG CAGTTTTTTT GGCATTACGC GGCATCCGAC CAGGAAATTC TGGAAGTCAA 
GACCACATTT TACCTAGAGA GATCTGTAAC CGCTTACTAA ATATTTTTCA TCCTACGGAC 
CCAGTGGCCT ACAGATTAGA ACCGTTGATT TTGAAACACT ACAGCAACAT TTCTCCAGTC 
CAGATCCACT GGTACAACAC TTCAAACCCT CTACCTTATG AGCATATGAA GCCAAATTTT 
CTCAACCCAG CAAAAGAACC TACCTCAGTT TCAGACAGTG AGAACATAGC AGCTATCCCA 
AGCCCTGTGA CCTCCCCCGT CTTATCTCGG CGCCACTATG GAGAATCTAT AACTAACATT 
GGCAAAGCAA GCATATTGGG GGCTGCTAGC ATTGGAAAGG GCCTTGGAGG AATGTTGTTC 
TCAAGATTTG GACGTTCTTC AGCATCACAG CCGTCTGAGC CATCTAAAGA CTCACTGGAA 
GATGATAAGA AGCCTTCAGC TTCACCCTCT ACCACCACCG TGGCAACGCA GACCCTGCCG 
CACAGTGGCT CCGGCTTCCT CGACTCCGCA TATTTCAGAC TTCAAGAATC GTTCTTTTAT 
CTCCCACAAC TTCTTTTTCC GGAAAATGTA ATGCAGAGTA AAGATGATAG CCTCGTGGAA 
CTGGAACACA GGATCGACTT TGAACTCAGA GAAGGCCTTG TGGAGAGCCG CTATTGGTCA 
GCCGTCACGT CGCACACTGC CTATTGGTCA TCCTTGGATG TCGCTCTTTT TCTTTTAACA 
TTCATGTACA AACACGAGCA TGATACCGAG GCCAAGCCCA GCCTAGGTTC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036184.1
CDS	133..2889
Translation	MNYPGRGAPRSPERNGRGGGAWELGSDAAGVFGGGGCCFEHQPGDSVPLSLLRAEPLHLAPGADDLSHLALDPCLSDETYDFSSAESGSSLRYYSEGESAGGGSSSSPPPPLVAQNSGGGGAAGGGPGDRKRARPGGAAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGAQARAAGRDGDRVCGPSSSFGEEDEEDSACGFCPRAAGPEPEMEELVTIEPVCVRGGLYEVDVTQGECYPVYWNQADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLSCFRGQQMQENFDIEVSKSLDGKDVVYHLPPFSLPSLFKWRGYKESTDATGLKRKRSQAVHSFKLSRNHVDWHSMDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTSHIVFVVHGIGQKMDQGRIIKNTAMMREAARKMEEKHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMMGWNPGGLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLREIEDRLHGLKAPSISQTPALKFKVENFFCMGSPLAVFLALRGIRPGNSGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPNFLNPAKEPTSVSDSENIAAIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSASQPSEPSKDSLEDDKKPSASPSTTTVATQTLPHSGSGFLDSAYFRLQESFFYLPQLLFPENVMQSKDDSLVELEHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDTEAKPSLGSL

Target ORF information:

RefSeq Version	NM_001042719.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042719.2

ORF Insert Sequence:

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGTTGTCT ACCACCTCCC TCCCTTCTCC CTCCCTTCTC TGTTCAAATG GAGAGGATAT 
AAGGAGAGCA CAGATGCCAC AGGTCTTAAG CGAAAGCGTT CCCAAGCTGT TCACAGTTTC 
AAGCTGAGTC GGAACCACGT GGACTGGCAC AGCATGGATG AAGTCTATCT CTACAGTGAC 
GCAACAACAT CCAAAATTGC GAGAACCGTT ACCCAAAAAC TGGGCTTTTC TAAAGCCTCC 
AGCAGTGGGA CCAGACTGCA CAGAGGTTAT GTAGAAGAAG CCACACTGGA AGACAAGCCG 
TCGCAGACTT CTCATATCGT GTTTGTTGTG CACGGCATTG GACAGAAGAT GGACCAAGGA 
AGAATCATCA AAAACACAGC CATGATGAGA GAGGCTGCAA GGAAAATGGA AGAAAAGCAT 
TTTTCCAACC ATGCAACACA TGTTGAATTT CTGCCTGTTG AATGGCGATC AAAGCTTACT 
CTTGATGGAG ACACAGTTGA TTCCATCACT CCTGACAAAG TGCGAGGCCT GAGGGACATG 
CTCAACAGCA GCGCGATGGA CATCATGTAC TACACCAGCC CACTCTACAG GGATGAGCTA 
GTTAAAGGCC TTCAGCAAGA GCTGAATCGA TTATATTCCC TTTTCTGTTC CCGGAATCCA 
GACTTTGAAG AGAAAGGGGG TAAAGTCTCA ATAGTGTCCC ATTCCTTGGG GTGTGTAATC 
ACTTATGACA TCATGATGGG CTGGAATCCA GGTGGACTCT ATGAGCAGCT GCTGCAGAAG 
GAGGAAGAGC TGCCCGACGA GCGGTGGATG AGCTATGAAG AGCGGCATCT TCTTGACGAG 
CTCTATATAA CAAAGCGACG GCTGAGGGAA ATAGAAGATC GGCTGCACGG ATTGAAGGCG 
CCATCCATAT CACAAACACC TGCCTTAAAA TTTAAGGTTG AAAATTTCTT CTGTATGGGA 
TCTCCGCTAG CAGTTTTTTT GGCATTACGC GGCATCCGAC CAGGAAATTC TGGAAGTCAA 
GACCACATTT TACCTAGAGA GATCTGTAAC CGCTTACTAA ATATTTTTCA TCCTACGGAC 
CCAGTGGCCT ACAGATTAGA ACCGTTGATT TTGAAACACT ACAGCAACAT TTCTCCAGTC 
CAGATCCACT GGTACAACAC TTCAAACCCT CTACCTTATG AGCATATGAA GCCAAATTTT 
CTCAACCCAG CAAAAGAACC TACCTCAGTT TCAGACAGTG AGAACATAGC AGCTATCCCA 
AGCCCTGTGA CCTCCCCCGT CTTATCTCGG CGCCACTATG GAGAATCTAT AACTAACATT 
GGCAAAGCAA GCATATTGGG GGCTGCTAGC ATTGGAAAGG GCCTTGGAGG AATGTTGTTC 
TCAAGATTTG GACGTTCTTC AGCATCACAG CCGTCTGAGC CATCTAAAGA CTCACTGGAA 
GATGATAAGA AGCCTTCAGC TTCACCCTCT ACCACCACCG TGGCAACGCA GACCCTGCCG 
CACAGTGGCT CCGGCTTCCT CGACTCCGCA TATTTCAGAC TTCAAGAATC GTTCTTTTAT 
CTCCCACAAC TTCTTTTTCC GGAAAATGTA ATGCAGAGTA AAGATGATAG CCTCGTGGAA 
CTGGAACACA GGATCGACTT TGAACTCAGA GAAGGCCTTG TGGAGAGCCG CTATTGGTCA 
GCCGTCACGT CGCACACTGC CTATTGGTCA TCCTTGGATG TCGCTCTTTT TCTTTTAACA 
TTCATGTACA AACACGAGCA TGATACCGAG GCCAAGCCCA GCCTAGGTTC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddhd1 ( NM_176845.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_789815.3 Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06258
Clone ID Related Accession (Same CDS sequence)	NM_176845.5
Accession Version	NM_176845.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	phospholipase DDHD1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107711.13, AK048690.1, BC043475.1 and AK036369.1. On Oct 1, 2013 this sequence version replaced NM_176845.4. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (2) is shorter, compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: DQ272481.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGCTGTTC ACAGTTTCAA GCTGAGTCGG AACCACGTGG ACTGGCACAG CATGGATGAA 
GTCTATCTCT ACAGTGACGC AACAACATCC AAAATTGCGA GAACCGTTAC CCAAAAACTG 
GGCTTTTCTA AAGCCTCCAG CAGTGGGACC AGACTGCACA GAGGTTATGT AGAAGAAGCC 
ACACTGGAAG ACAAGCCGTC GCAGACTTCT CATATCGTGT TTGTTGTGCA CGGCATTGGA 
CAGAAGATGG ACCAAGGAAG AATCATCAAA AACACAGCCA TGATGAGAGA GGCTGCAAGG 
AAAATGGAAG AAAAGCATTT TTCCAACCAT GCAACACATG TTGAATTTCT GCCTGTTGAA 
TGGCGATCAA AGCTTACTCT TGATGGAGAC ACAGTTGATT CCATCACTCC TGACAAAGTG 
CGAGGCCTGA GGGACATGCT CAACAGCAGC GCGATGGACA TCATGTACTA CACCAGCCCA 
CTCTACAGGG ATGAGCTAGT TAAAGGCCTT CAGCAAGAGC TGAATCGATT ATATTCCCTT 
TTCTGTTCCC GGAATCCAGA CTTTGAAGAG AAAGGGGGTA AAGTCTCAAT AGTGTCCCAT 
TCCTTGGGGT GTGTAATCAC TTATGACATC ATGATGGGCT GGAATCCAGG TGGACTCTAT 
GAGCAGCTGC TGCAGAAGGA GGAAGAGCTG CCCGACGAGC GGTGGATGAG CTATGAAGAG 
CGGCATCTTC TTGACGAGCT CTATATAACA AAGCGACGGC TGAGGGAAAT AGAAGATCGG 
CTGCACGGAT TGAAGGCGCC ATCCATATCA CAAACACCTG CCTTAAAATT TAAGGTTGAA 
AATTTCTTCT GTATGGGATC TCCGCTAGCA GTTTTTTTGG CATTACGCGG CATCCGACCA 
GGAAATTCTG GAAGTCAAGA CCACATTTTA CCTAGAGAGA TCTGTAACCG CTTACTAAAT 
ATTTTTCATC CTACGGACCC AGTGGCCTAC AGATTAGAAC CGTTGATTTT GAAACACTAC 
AGCAACATTT CTCCAGTCCA GATCCACTGG TACAACACTT CAAACCCTCT ACCTTATGAG 
CATATGAAGC CAAATTTTCT CAACCCAGCA AAAGAACCTA CCTCAGTTTC AGACAGTGAG 
AACATAGCAG CTATCCCAAG CCCTGTGACC TCCCCCGTCT TATCTCGGCG CCACTATGGA 
GAATCTATAA CTAACATTGG CAAAGCAAGC ATATTGGGGG CTGCTAGCAT TGGAAAGGGC 
CTTGGAGGAA TGTTGTTCTC AAGATTTGGA CGTTCTTCAG CATCACAGCC GTCTGAGCCA 
TCTAAAGACT CACTGGAAGA TGATAAGAAG CCTTCAGCTT CACCCTCTAC CACCACCGTG 
GCAACGCAGA CCCTGCCGCA CAGTGGCTCC GGCTTCCTCG ACTCCGCATA TTTCAGACTT 
CAAGAATCGT TCTTTTATCT CCCACAACTT CTTTTTCCGG AAAATGTAAT GCAGAGTAAA 
GATGATAGCC TCGTGGAACT GGAACACAGG ATCGACTTTG AACTCAGAGA AGGCCTTGTG 
GAGAGCCGCT ATTGGTCAGC CGTCACGTCG CACACTGCCT ATTGGTCATC CTTGGATGTC 
GCTCTTTTTC TTTTAACATT CATGTACAAA CACGAGCATG ATACCGAGGC CAAGCCCAGC 
CTAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_789815.3
CDS	133..2787
Translation	MNYPGRGAPRSPERNGRGGGAWELGSDAAGVFGGGGCCFEHQPGDSVPLSLLRAEPLHLAPGADDLSHLALDPCLSDETYDFSSAESGSSLRYYSEGESAGGGSSSSPPPPLVAQNSGGGGAAGGGPGDRKRARPGGAAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGAQARAAGRDGDRVCGPSSSFGEEDEEDSACGFCPRAAGPEPEMEELVTIEPVCVRGGLYEVDVTQGECYPVYWNQADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLSCFRGQQMQENFDIEVSKSLDGKDAVHSFKLSRNHVDWHSMDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTSHIVFVVHGIGQKMDQGRIIKNTAMMREAARKMEEKHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMMGWNPGGLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLREIEDRLHGLKAPSISQTPALKFKVENFFCMGSPLAVFLALRGIRPGNSGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPNFLNPAKEPTSVSDSENIAAIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSASQPSEPSKDSLEDDKKPSASPSTTTVATQTLPHSGSGFLDSAYFRLQESFFYLPQLLFPENVMQSKDDSLVELEHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDTEAKPSLGSL

Target ORF information:

RefSeq Version	NM_176845.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176845.5

ORF Insert Sequence:

ATGAACTACC CGGGCCGCGG CGCCCCACGG AGCCCCGAGC GTAACGGCCG CGGCGGCGGT 
GCCTGGGAGC TGGGCTCGGA CGCGGCGGGC GTGTTCGGCG GCGGCGGCTG CTGCTTCGAG 
CACCAGCCGG GCGACAGCGT GCCGCTGTCG CTGCTGCGCG CCGAGCCGCT GCACCTGGCA 
CCGGGCGCCG ACGACCTGAG CCACCTGGCT CTGGACCCGT GCCTCAGCGA CGAGACCTAC 
GACTTCAGCT CGGCCGAGTC CGGCTCGTCG TTGCGCTACT ACAGCGAGGG CGAGAGCGCG 
GGCGGCGGCA GCTCTTCTTC GCCTCCGCCG CCGCTCGTCG CCCAGAACTC GGGGGGCGGC 
GGGGCGGCGG GAGGGGGCCC CGGGGACAGG AAGCGCGCCC GGCCCGGCGG CGCAGCCGCC 
CGGCACCGCT ACGAGGTGGT GACGGAGCTG GGCCCCGAGG AGGTGCGCTG GTTCTACAAG 
GAAGACAAGA AGACCTGGAA GCCCTTCATC GGCTACGACT CTCTGCGCAT CGAGCTCGCC 
TTCCGGACCC TGCTGCAGAC CACGGGCGCC CAGGCCCGGG CGGCGGGCCG CGATGGAGAC 
CGGGTGTGTG GCCCAAGCTC CAGCTTCGGG GAGGAAGACG AGGAGGACAG CGCCTGCGGC 
TTCTGCCCGC GCGCGGCGGG GCCTGAGCCG GAGATGGAGG AGCTGGTGAC CATCGAGCCG 
GTGTGCGTGC GCGGCGGCCT CTACGAGGTG GATGTCACTC AAGGAGAGTG CTACCCGGTG 
TACTGGAACC AAGCTGATAA AATACCAGTA ATGCGTGGAC AGTGGTTTAT TGATGGGACG 
TGGCAACCTC TAGAAGAAGA AGAAAGTAAT TTAATTGAGC AAGAACATCT CAGTTGTTTT 
AGGGGTCAGC AGATGCAAGA AAATTTTGAT ATTGAAGTGT CAAAATCCTT AGATGGAAAA 
GACGCTGTTC ACAGTTTCAA GCTGAGTCGG AACCACGTGG ACTGGCACAG CATGGATGAA 
GTCTATCTCT ACAGTGACGC AACAACATCC AAAATTGCGA GAACCGTTAC CCAAAAACTG 
GGCTTTTCTA AAGCCTCCAG CAGTGGGACC AGACTGCACA GAGGTTATGT AGAAGAAGCC 
ACACTGGAAG ACAAGCCGTC GCAGACTTCT CATATCGTGT TTGTTGTGCA CGGCATTGGA 
CAGAAGATGG ACCAAGGAAG AATCATCAAA AACACAGCCA TGATGAGAGA GGCTGCAAGG 
AAAATGGAAG AAAAGCATTT TTCCAACCAT GCAACACATG TTGAATTTCT GCCTGTTGAA 
TGGCGATCAA AGCTTACTCT TGATGGAGAC ACAGTTGATT CCATCACTCC TGACAAAGTG 
CGAGGCCTGA GGGACATGCT CAACAGCAGC GCGATGGACA TCATGTACTA CACCAGCCCA 
CTCTACAGGG ATGAGCTAGT TAAAGGCCTT CAGCAAGAGC TGAATCGATT ATATTCCCTT 
TTCTGTTCCC GGAATCCAGA CTTTGAAGAG AAAGGGGGTA AAGTCTCAAT AGTGTCCCAT 
TCCTTGGGGT GTGTAATCAC TTATGACATC ATGATGGGCT GGAATCCAGG TGGACTCTAT 
GAGCAGCTGC TGCAGAAGGA GGAAGAGCTG CCCGACGAGC GGTGGATGAG CTATGAAGAG 
CGGCATCTTC TTGACGAGCT CTATATAACA AAGCGACGGC TGAGGGAAAT AGAAGATCGG 
CTGCACGGAT TGAAGGCGCC ATCCATATCA CAAACACCTG CCTTAAAATT TAAGGTTGAA 
AATTTCTTCT GTATGGGATC TCCGCTAGCA GTTTTTTTGG CATTACGCGG CATCCGACCA 
GGAAATTCTG GAAGTCAAGA CCACATTTTA CCTAGAGAGA TCTGTAACCG CTTACTAAAT 
ATTTTTCATC CTACGGACCC AGTGGCCTAC AGATTAGAAC CGTTGATTTT GAAACACTAC 
AGCAACATTT CTCCAGTCCA GATCCACTGG TACAACACTT CAAACCCTCT ACCTTATGAG 
CATATGAAGC CAAATTTTCT CAACCCAGCA AAAGAACCTA CCTCAGTTTC AGACAGTGAG 
AACATAGCAG CTATCCCAAG CCCTGTGACC TCCCCCGTCT TATCTCGGCG CCACTATGGA 
GAATCTATAA CTAACATTGG CAAAGCAAGC ATATTGGGGG CTGCTAGCAT TGGAAAGGGC 
CTTGGAGGAA TGTTGTTCTC AAGATTTGGA CGTTCTTCAG CATCACAGCC GTCTGAGCCA 
TCTAAAGACT CACTGGAAGA TGATAAGAAG CCTTCAGCTT CACCCTCTAC CACCACCGTG 
GCAACGCAGA CCCTGCCGCA CAGTGGCTCC GGCTTCCTCG ACTCCGCATA TTTCAGACTT 
CAAGAATCGT TCTTTTATCT CCCACAACTT CTTTTTCCGG AAAATGTAAT GCAGAGTAAA 
GATGATAGCC TCGTGGAACT GGAACACAGG ATCGACTTTG AACTCAGAGA AGGCCTTGTG 
GAGAGCCGCT ATTGGTCAGC CGTCACGTCG CACACTGCCT ATTGGTCATC CTTGGATGTC 
GCTCTTTTTC TTTTAACATT CATGTACAAA CACGAGCATG ATACCGAGGC CAAGCCCAGC 
CTAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddhd1 ( NM_001284399.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001271328.1 Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu08620
Clone ID Related Accession (Same CDS sequence)	NM_001284399.1
Accession Version	NM_001284399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	phospholipase DDHD1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107711.13, AK048690.1, CK619141.1, BC043475.1 and AK036369.1. Transcript Variant: This variant (4) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 3. The encoded isoform (4) has a shorter N-terminus, compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: AK155294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAG TCTATCTCTA CAGTGACGCA ACAACATCCA AAATTGCGAG AACCGTTACC 
CAAAAACTGG GCTTTTCTAA AGCCTCCAGC AGTGGGACCA GACTGCACAG AGGTTATGTA 
GAAGAAGCCA CACTGGAAGA CAAGCCGTCG CAGACTTCTC ATATCGTGTT TGTTGTGCAC 
GGCATTGGAC AGAAGATGGA CCAAGGAAGA ATCATCAAAA ACACAGCCAT GATGAGAGAG 
GCTGCAAGGA AAATGGAAGA AAAGCATTTT TCCAACCATG CAACACATGT TGAATTTCTG 
CCTGTTGAAT GGCGATCAAA GCTTACTCTT GATGGAGACA CAGTTGATTC CATCACTCCT 
GACAAAGTGC GAGGCCTGAG GGACATGCTC AACAGCAGCG CGATGGACAT CATGTACTAC 
ACCAGCCCAC TCTACAGGGA TGAGCTAGTT AAAGGCCTTC AGCAAGAGCT GAATCGATTA 
TATTCCCTTT TCTGTTCCCG GAATCCAGAC TTTGAAGAGA AAGGGGGTAA AGTCTCAATA 
GTGTCCCATT CCTTGGGGTG TGTAATCACT TATGACATCA TGATGGGCTG GAATCCAGGT 
GGACTCTATG AGCAGCTGCT GCAGAAGGAG GAAGAGCTGC CCGACGAGCG GTGGATGAGC 
TATGAAGAGC GGCATCTTCT TGACGAGCTC TATATAACAA AGCGACGGCT GAGGGAAATA 
GAAGATCGGC TGCACGGATT GAAGGCGCCA TCCATATCAC AAACACCTGC CTTAAAATTT 
AAGGTTGAAA ATTTCTTCTG TATGGGATCT CCGCTAGCAG TTTTTTTGGC ATTACGCGGC 
ATCCGACCAG GAAATTCTGG AAGTCAAGAC CACATTTTAC CTAGAGAGAT CTGTAACCGC 
TTACTAAATA TTTTTCATCC TACGGACCCA GTGGCCTACA GATTAGAACC GTTGATTTTG 
AAACACTACA GCAACATTTC TCCAGTCCAG ATCCACTGGT ACAACACTTC AAACCCTCTA 
CCTTATGAGC ATATGAAGCC AAATTTTCTC AACCCAGCAA AAGAACCTAC CTCAGTTTCA 
GACAGTGAGA ACATAGCAGC TATCCCAAGC CCTGTGACCT CCCCCGTCTT ATCTCGGCGC 
CACTATGGAG AATCTATAAC TAACATTGGC AAAGCAAGCA TATTGGGGGC TGCTAGCATT 
GGAAAGGGCC TTGGAGGAAT GTTGTTCTCA AGATTTGGAC GTTCTTCAGC ATCACAGCCG 
TCTGAGCCAT CTAAAGACTC ACTGGAAGAT GATAAGAAGC CTTCAGCTTC ACCCTCTACC 
ACCACCGTGG CAACGCAGAC CCTGCCGCAC AGTGGCTCCG GCTTCCTCGA CTCCGCATAT 
TTCAGACTTC AAGAATCGTT CTTTTATCTC CCACAACTTC TTTTTCCGGA AAATGTAATG 
CAGAGTAAAG ATGATAGCCT CGTGGAACTG GAACACAGGA TCGACTTTGA ACTCAGAGAA 
GGCCTTGTGG AGAGCCGCTA TTGGTCAGCC GTCACGTCGC ACACTGCCTA TTGGTCATCC 
TTGGATGTCG CTCTTTTTCT TTTAACATTC ATGTACAAAC ACGAGCATGA TACCGAGGCC 
AAGCCCAGCC TAGGTTCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271328.1
CDS	1218..2861
Translation	MDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTSHIVFVVHGIGQKMDQGRIIKNTAMMREAARKMEEKHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMMGWNPGGLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLREIEDRLHGLKAPSISQTPALKFKVENFFCMGSPLAVFLALRGIRPGNSGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPNFLNPAKEPTSVSDSENIAAIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSASQPSEPSKDSLEDDKKPSASPSTTTVATQTLPHSGSGFLDSAYFRLQESFFYLPQLLFPENVMQSKDDSLVELEHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDTEAKPSLGSL

Target ORF information:

RefSeq Version	NM_001284399.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DDHD domain containing 1 (Ddhd1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284399.1

ORF Insert Sequence:

ATGGATGAAG TCTATCTCTA CAGTGACGCA ACAACATCCA AAATTGCGAG AACCGTTACC 
CAAAAACTGG GCTTTTCTAA AGCCTCCAGC AGTGGGACCA GACTGCACAG AGGTTATGTA 
GAAGAAGCCA CACTGGAAGA CAAGCCGTCG CAGACTTCTC ATATCGTGTT TGTTGTGCAC 
GGCATTGGAC AGAAGATGGA CCAAGGAAGA ATCATCAAAA ACACAGCCAT GATGAGAGAG 
GCTGCAAGGA AAATGGAAGA AAAGCATTTT TCCAACCATG CAACACATGT TGAATTTCTG 
CCTGTTGAAT GGCGATCAAA GCTTACTCTT GATGGAGACA CAGTTGATTC CATCACTCCT 
GACAAAGTGC GAGGCCTGAG GGACATGCTC AACAGCAGCG CGATGGACAT CATGTACTAC 
ACCAGCCCAC TCTACAGGGA TGAGCTAGTT AAAGGCCTTC AGCAAGAGCT GAATCGATTA 
TATTCCCTTT TCTGTTCCCG GAATCCAGAC TTTGAAGAGA AAGGGGGTAA AGTCTCAATA 
GTGTCCCATT CCTTGGGGTG TGTAATCACT TATGACATCA TGATGGGCTG GAATCCAGGT 
GGACTCTATG AGCAGCTGCT GCAGAAGGAG GAAGAGCTGC CCGACGAGCG GTGGATGAGC 
TATGAAGAGC GGCATCTTCT TGACGAGCTC TATATAACAA AGCGACGGCT GAGGGAAATA 
GAAGATCGGC TGCACGGATT GAAGGCGCCA TCCATATCAC AAACACCTGC CTTAAAATTT 
AAGGTTGAAA ATTTCTTCTG TATGGGATCT CCGCTAGCAG TTTTTTTGGC ATTACGCGGC 
ATCCGACCAG GAAATTCTGG AAGTCAAGAC CACATTTTAC CTAGAGAGAT CTGTAACCGC 
TTACTAAATA TTTTTCATCC TACGGACCCA GTGGCCTACA GATTAGAACC GTTGATTTTG 
AAACACTACA GCAACATTTC TCCAGTCCAG ATCCACTGGT ACAACACTTC AAACCCTCTA 
CCTTATGAGC ATATGAAGCC AAATTTTCTC AACCCAGCAA AAGAACCTAC CTCAGTTTCA 
GACAGTGAGA ACATAGCAGC TATCCCAAGC CCTGTGACCT CCCCCGTCTT ATCTCGGCGC 
CACTATGGAG AATCTATAAC TAACATTGGC AAAGCAAGCA TATTGGGGGC TGCTAGCATT 
GGAAAGGGCC TTGGAGGAAT GTTGTTCTCA AGATTTGGAC GTTCTTCAGC ATCACAGCCG 
TCTGAGCCAT CTAAAGACTC ACTGGAAGAT GATAAGAAGC CTTCAGCTTC ACCCTCTACC 
ACCACCGTGG CAACGCAGAC CCTGCCGCAC AGTGGCTCCG GCTTCCTCGA CTCCGCATAT 
TTCAGACTTC AAGAATCGTT CTTTTATCTC CCACAACTTC TTTTTCCGGA AAATGTAATG 
CAGAGTAAAG ATGATAGCCT CGTGGAACTG GAACACAGGA TCGACTTTGA ACTCAGAGAA 
GGCCTTGTGG AGAGCCGCTA TTGGTCAGCC GTCACGTCGC ACACTGCCTA TTGGTCATCC 
TTGGATGTCG CTCTTTTTCT TTTAACATTC ATGTACAAAC ACGAGCATGA TACCGAGGCC 
AAGCCCAGCC TAGGTTCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Spastic Paraplegia-Associated Phospholipase DDHD1 Is a Primary Brain Phosphatidylinositol Lipase.
Biochemistry57(39)5759-5767(2018 10)
Inloes JM,Jing H,Cravatt BF

Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.
The Journal of biological chemistry289(16)11497-511(2014 Apr)
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Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage.
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Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
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Ddhd1 cDNA ORF clone, Mus musculus(house mouse)

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