Spred2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spred2
Entrez Gene ID	114716
Full Name	sprouty-related, EVH1 domain containing 2
Synonyms	C79158
General protein information	Preferred Names sprouty-related, EVH1 domain containing 2 Names sprouty-related, EVH1 domain-containing protein 2 sprouty protein with EVH-1 domain 2, related sequence
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A3.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006514456.4	XP_006514519.1	sprouty-related, EVH1 domain-containing protein 2 isoform X1
XM_006514457.3	XP_006514520.1	sprouty-related, EVH1 domain-containing protein 2 isoform X2
NM_033523.4	NP_277058.1	sprouty-related, EVH1 domain-containing protein 2
XM_006514458.3	XP_006514521.1	sprouty-related, EVH1 domain-containing protein 2 isoform X3
XM_006514456.3	XP_006514519.1	sprouty-related, EVH1 domain-containing protein 2 isoform X1
XM_006514457.4	XP_006514520.1	sprouty-related, EVH1 domain-containing protein 2 isoform X2
XM_006514458.4	XP_006514521.1	sprouty-related, EVH1 domain-containing protein 2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPRED2	NP_861449.2
Gallus gallus (chicken)	SPRED2	XP_419341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012085	XP_320440.4
Pan troglodytes (chimpanzee)	SPRED2	XP_515518.3
Macaca mulatta (Rhesus monkey)	SPRED2	XP_001091600.1
Bos taurus (cattle)	SPRED2	XP_005212957.1
Mus musculus (house mouse)	Spred2	NP_277058.1
Rattus norvegicus (Norway rat)	Spred2	NP_001040559.1
Canis lupus familiaris (dog)	SPRED2	XP_538517.2
Danio rerio (zebrafish)	spred2b	NP_998399.1
Xenopus tropicalis (tropical clawed frog)	spred2	NP_001011032.1
Drosophila melanogaster (fruit fly)	Spred	NP_610988.1

Gene Ontology
Bibliography

Related articles in PubMed

Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice.
Ohkura T, Yoshimura T, Fujisawa M, Ohara T, Marutani R, Usami K, Matsukawa A
Frontiers in immunology1017(2019)

SPRED2 deficiency may lead to lung ischemia-reperfusion injury via ERK1/2 signaling pathway activation.
Okada M, Yamane M, Yamamoto S, Otani S, Miyoshi K, Sugimoto S, Matsukawa A, Toyooka S, Oto T, Miyoshi S
Surgery today48(12)1089-1095(2018 Dec)

Spred2 Deficiency Exacerbates D-Galactosamine/Lipopolysaccharide -induced Acute Liver Injury in Mice via Increased Production of TNFα.
Yang X, Fujisawa M, Yoshimura T, Ohara T, Sato M, Mino M, San TH, Gao T, Kunkel SL, Matsukawa A
Scientific reports8(1)188(2018 01)

Spred2-deficiecy Protects Mice from Polymicrobial Septic Peritonitis by Enhancing Inflammation and Bacterial Clearance.
Itakura J, Sato M, Ito T, Mino M, Fushimi S, Takahashi S, Yoshimura T, Matsukawa A
Scientific reports7(1)12833(2017 10)

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spred2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spred2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45035	XM_006514456.4 Latest version!	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu45036	XM_006514457.4 Latest version!	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu45037	XM_006514458.4 Latest version!	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu08940	NM_033523.4 Latest version!	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OMu45035	XM_006514456.3	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu45036	XM_006514457.3	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu45037	XM_006514458.3	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spred2 ( XM_006514456.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514519.1 Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45035
Clone ID Related Accession (Same CDS sequence)	XM_006514456.4 , XM_006514456.3
Accession Version	XM_006514456.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514456.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCT TTGAAAGGTT TTCAGTTGGG AATCCCAGGG ATGAAGGCTC CAGCCAAGTC 
ATAGGAGACC CAGACAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514519.1
CDS	17070..18353
Translation	MSAFERFSVGNPRDEGSSQVIGDPDSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514456.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514456.4

ORF Insert Sequence:

ATGTCAGCCT TTGAAAGGTT TTCAGTTGGG AATCCCAGGG ATGAAGGCTC CAGCCAAGTC 
ATAGGAGACC CAGACAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( XM_006514456.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514519.1 Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45035
Clone ID Related Accession (Same CDS sequence)	XM_006514456.4 , XM_006514456.3
Accession Version	XM_006514456.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCT TTGAAAGGTT TTCAGTTGGG AATCCCAGGG ATGAAGGCTC CAGCCAAGTC 
ATAGGAGACC CAGACAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514519.1
CDS	17013..18296
Translation	MSAFERFSVGNPRDEGSSQVIGDPDSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514456.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514456.3

ORF Insert Sequence:

ATGTCAGCCT TTGAAAGGTT TTCAGTTGGG AATCCCAGGG ATGAAGGCTC CAGCCAAGTC 
ATAGGAGACC CAGACAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( XM_006514457.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514520.1 Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45036
Clone ID Related Accession (Same CDS sequence)	XM_006514457.3 , XM_006514457.4
Accession Version	XM_006514457.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGGTT TTACAGAGCT CACTAGTGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514520.1
CDS	895..2127
Translation	MIGFTELTSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514457.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514457.3

ORF Insert Sequence:

ATGATTGGTT TTACAGAGCT CACTAGTGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( XM_006514457.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514520.1 Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45036
Clone ID Related Accession (Same CDS sequence)	XM_006514457.3 , XM_006514457.4
Accession Version	XM_006514457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGGTT TTACAGAGCT CACTAGTGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514520.1
CDS	893..2125
Translation	MIGFTELTSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514457.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514457.4

ORF Insert Sequence:

ATGATTGGTT TTACAGAGCT CACTAGTGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( XM_006514458.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514521.1 Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.

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CloneID	OMu45037
Clone ID Related Accession (Same CDS sequence)	XM_006514458.3 , XM_006514458.4
Accession Version	XM_006514458.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CGGGCAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514521.1
CDS	11369..12592
Translation	MASPGSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514458.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related, EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514458.3

ORF Insert Sequence:

ATGGCATCTC CGGGCAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( XM_006514458.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514521.1 Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45037
Clone ID Related Accession (Same CDS sequence)	XM_006514458.3 , XM_006514458.4
Accession Version	XM_006514458.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514458.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CGGGCAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514521.1
CDS	11452..12675
Translation	MASPGSDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	XM_006514458.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514458.4

ORF Insert Sequence:

ATGGCATCTC CGGGCAGTGA CAGCTATATT GTGCGTGTCA AGGCTGTGGT TATGACCAGA 
GATGACTCCA GCGGGGGATG GTTCCCACAG GAAGGAGGCG GGATCAGTCG CGTCGGCGTG 
TGTAAGGTCA TGCACCCTGA AGGCAACGGA CGAAGCGGCT TTCTCATCCA TGGCGAGCGA 
CAGAAAGACA AACTGGTGGT ATTGGAATGC TATGTCAGAA AGGACTTGGT CTACACCAAA 
GCCAATCCGA CGTTTCATCA TTGGAAGGTT GATAACAGGA AGTTTGGACT TACTTTCCAA 
AGTCCTGCAG ATGCACGAGC CTTTGACAGG GGCGTGAGAA AAGCCATTGA AGACCTTATA 
GAAGGTTCAA CGACCTCCTC TTCCACTCTC CATAACGAAG CTGAGCTCGG AGACGATGAC 
GTTTTCACGA CAGCTACGGA CAGTTCTTCT AATTCCTCGC AGAAGAGGGA GCCGACTACG 
AGGACAATCT CCTCCCCCAC GTCCTGTGAG CACCGGAAGA TTTATACCCT TGACCCATAC 
CCCATGGACC ATTACCACCC TGACCAGCGG TTGCCGCGGT CCTACCCCCA GGTCACCTTC 
CCAGAAGATG ATGAAGAAAT TGTACGCATC AACCCCCGAG AGAAGATCTG GATGACCGGT 
TATGAAGACT ACCGGCACGC GCCGGTTCGC GGCAAATACT TAGACACCAC AGAAGACGCG 
GACTCCTACG TGCGCTTCGC CAAGGGCGAA GTCCCCAAAC ACGAATATAC CTATCCCTAT 
GTTGATTCTT CGGACTTCGG CTTCGGGGAG GATCCCAAAG GTAGTGTGAT CAAGACACAG 
CCGCCCAGGG CCAAGTCCCG TCGGCGGAAG GAGAACGGCG AACGGTCGCG GTGTGTGTAC 
TGCAGGGATA TGTTTAATCA CGAAGAGAAC CGAAGGGGCC ACTGCCAAGA CGCGCCCGAC 
GCCGTGAGAA CTTGCATTCG CCGGGTGAGC TGTATGTGGT GCGCGGACAG CATGCTGTAC 
CACTGTATGT CCGACCCCGA GGGAGACTAC ACTGACCCTT GTTCGTGTGA CACAAGCGAT 
GAGAAGTTTT GCCTCCGGTG GATGGCTCTA ATTGCCTTGT CTTTCCTGGC CCCTTGTATG 
TGCTGTTACC TGCCCCTCCG GGCCTGCCAC CGCTGTGGAG TGATGTGCAG GTGCTGTGGT 
GGGAAGCACA AAGCCGCCGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred2 ( NM_033523.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_277058.1 Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), mRNA.

Price & Availability↑

CloneID	OMu08940
Clone ID Related Accession (Same CDS sequence)	NM_033523.4
Accession Version	NM_033523.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645609.21 and AL672084.10. On May 1, 2007 this sequence version replaced NM_033523.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036430.1, AK157817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACCGAAG AAACACACCC GGACGATGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277058.1
CDS	490..1722
Translation	MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Target ORF information:

RefSeq Version	NM_033523.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty-related EVH1 domain containing 2 (Spred2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033523.4

ORF Insert Sequence:

ATGACCGAAG AAACACACCC GGACGATGAC AGCTATATTG TGCGTGTCAA GGCTGTGGTT 
ATGACCAGAG ATGACTCCAG CGGGGGATGG TTCCCACAGG AAGGAGGCGG GATCAGTCGC 
GTCGGCGTGT GTAAGGTCAT GCACCCTGAA GGCAACGGAC GAAGCGGCTT TCTCATCCAT 
GGCGAGCGAC AGAAAGACAA ACTGGTGGTA TTGGAATGCT ATGTCAGAAA GGACTTGGTC 
TACACCAAAG CCAATCCGAC GTTTCATCAT TGGAAGGTTG ATAACAGGAA GTTTGGACTT 
ACTTTCCAAA GTCCTGCAGA TGCACGAGCC TTTGACAGGG GCGTGAGAAA AGCCATTGAA 
GACCTTATAG AAGGTTCAAC GACCTCCTCT TCCACTCTCC ATAACGAAGC TGAGCTCGGA 
GACGATGACG TTTTCACGAC AGCTACGGAC AGTTCTTCTA ATTCCTCGCA GAAGAGGGAG 
CCGACTACGA GGACAATCTC CTCCCCCACG TCCTGTGAGC ACCGGAAGAT TTATACCCTT 
GACCCATACC CCATGGACCA TTACCACCCT GACCAGCGGT TGCCGCGGTC CTACCCCCAG 
GTCACCTTCC CAGAAGATGA TGAAGAAATT GTACGCATCA ACCCCCGAGA GAAGATCTGG 
ATGACCGGTT ATGAAGACTA CCGGCACGCG CCGGTTCGCG GCAAATACTT AGACACCACA 
GAAGACGCGG ACTCCTACGT GCGCTTCGCC AAGGGCGAAG TCCCCAAACA CGAATATACC 
TATCCCTATG TTGATTCTTC GGACTTCGGC TTCGGGGAGG ATCCCAAAGG TAGTGTGATC 
AAGACACAGC CGCCCAGGGC CAAGTCCCGT CGGCGGAAGG AGAACGGCGA ACGGTCGCGG 
TGTGTGTACT GCAGGGATAT GTTTAATCAC GAAGAGAACC GAAGGGGCCA CTGCCAAGAC 
GCGCCCGACG CCGTGAGAAC TTGCATTCGC CGGGTGAGCT GTATGTGGTG CGCGGACAGC 
ATGCTGTACC ACTGTATGTC CGACCCCGAG GGAGACTACA CTGACCCTTG TTCGTGTGAC 
ACAAGCGATG AGAAGTTTTG CCTCCGGTGG ATGGCTCTAA TTGCCTTGTC TTTCCTGGCC 
CCTTGTATGT GCTGTTACCT GCCCCTCCGG GCCTGCCACC GCTGTGGAGT GATGTGCAGG 
TGCTGTGGTG GGAAGCACAA AGCCGCCGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice.
Frontiers in immunology1017(2019)
Ohkura T,Yoshimura T,Fujisawa M,Ohara T,Marutani R,Usami K,Matsukawa A

SPRED2 deficiency may lead to lung ischemia-reperfusion injury via ERK1/2 signaling pathway activation.
Surgery today48(12)1089-1095(2018 Dec)
Okada M,Yamane M,Yamamoto S,Otani S,Miyoshi K,Sugimoto S,Matsukawa A,Toyooka S,Oto T,Miyoshi S

Spred2 Deficiency Exacerbates D-Galactosamine/Lipopolysaccharide -induced Acute Liver Injury in Mice via Increased Production of TNFα.
Scientific reports8(1)188(2018 01)
Yang X,Fujisawa M,Yoshimura T,Ohara T,Sato M,Mino M,San TH,Gao T,Kunkel SL,Matsukawa A

Spred2-deficiecy Protects Mice from Polymicrobial Septic Peritonitis by Enhancing Inflammation and Bacterial Clearance.
Scientific reports7(1)12833(2017 10)
Itakura J,Sato M,Ito T,Mino M,Fushimi S,Takahashi S,Yoshimura T,Matsukawa A

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

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Spred2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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