USP14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP14
Entrez Gene ID	9097
Full Name	ubiquitin specific peptidase 14
Synonyms	TGT
General protein information	Preferred Names ubiquitin specific peptidase 14 Names ubiquitin carboxyl-terminal hydrolase 14 deubiquitinating enzyme 14 tRNA-guanine transglycosylase, 60-kD subunit ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) ubiquitin specific protease 14 (tRNA-guanine transglycosylase) ubiquitin thioesterase 14 ubiquitin thiolesterase 14 ubiquitin-specific processing protease 14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.32
Summary	This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	607274

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037334.2	NP_001032411.1	ubiquitin carboxyl-terminal hydrolase 14 isoform b
NM_005151.3	NP_005142.1	ubiquitin carboxyl-terminal hydrolase 14 isoform a
NM_001037334.1	NP_001032411.1	ubiquitin carboxyl-terminal hydrolase 14 isoform b
NM_005151.4	NP_005142.1	ubiquitin carboxyl-terminal hydrolase 14 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP14	NP_005142.1
Canis lupus familiaris (dog)	USP14	XP_537306.2
Gallus gallus (chicken)	USP14	XP_004939812.1
Xenopus tropicalis (tropical clawed frog)	usp14	NP_001005641.1
Bos taurus (cattle)	USP14	NP_001068657.1
Caenorhabditis elegans (roundworm)	usp-14	NP_497006.1
Schizosaccharomyces pombe (fission yeast)	ubp6	NP_594117.2
Pan troglodytes (chimpanzee)	USP14	NP_001038961.1
Neurospora crassa	NCU01919	XP_965550.1
Magnaporthe oryzae (rice blast fungus)	MGG_02970	XP_003720730.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009348	XP_310014.4
Kluyveromyces lactis	KLLA0A10791g	XP_451470.1
Eremothecium gossypii	AGOS_AEL029W	NP_984832.2
Drosophila melanogaster (fruit fly)	CG5384	NP_001260321.1
Saccharomyces cerevisiae (baker's yeast)	UBP6	NP_116665.1
Arabidopsis thaliana (thale cress)	UBP7	NP_566680.2
Oryza sativa (rice)	Os01g0550100	NP_001043299.1
Macaca mulatta (Rhesus monkey)	USP14	XP_001090784.1
Mus musculus (house mouse)	Usp14	NP_067497.2
Rattus norvegicus (Norway rat)	Usp14	NP_001008302.1
Danio rerio (zebrafish)	usp14	NP_956267.1
Arabidopsis thaliana (thale cress)	UBP6	NP_564596.1

Gene Ontology
Bibliography

Related articles in PubMed

USP14-mediated IκBα degradation exacerbates NF-κB activation and IL-1β-stimulated chondrocyte dedifferentiation.
Li M, Zhao J, Jia L
Life sciences218147-152(2019 Feb)

USP14 promotes K63-linked RIG-I deubiquitination and suppresses antiviral immune responses.
Li H, Zhao Z, Ling J, Pan L, Zhao X, Zhu H, Yu J, Xie B, Shen J, Chen W
European journal of immunology49(1)42-53(2019 01)

UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells.
Kim HT, Goldberg AL
Proceedings of the National Academy of Sciences of the United States of America115(50)E11642-E11650(2018 12)

USP14 inhibition corrects an
Chakraborty J, von Stockum S, Marchesan E, Caicci F, Ferrari V, Rakovic A, Klein C, Antonini A, Bubacco L, Ziviani E
EMBO molecular medicine10(11)(2018 11)

USP14 as a novel prognostic marker promotes cisplatin resistance via Akt/ERK signaling pathways in gastric cancer.
Fu Y, Ma G, Liu G, Li B, Li H, Hao X, Liu L
Cancer medicine7(11)5577-5588(2018 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following USP14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16474	NM_005151.4 Latest version!	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu16417	NM_001037334.2 Latest version!	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu16417	NM_001037334.1	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu16474	NM_005151.3	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP14 ( NM_005151.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005142.1 Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.

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CloneID	OHu16474
Clone ID Related Accession (Same CDS sequence)	NM_005151.3 , NM_005151.4
Accession Version	NM_005151.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512835200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 14 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717018.1, BC003556.1, CA438203.1, BU161860.1 and AK098497.1. On Dec 1, 2005 this sequence version replaced NM_005151.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.172991.1, SRR1660807.106677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATGGAAT GACTCTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCAG CCAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCATCTGCT 
ATGGAGTTAC CATGTGGATT GACAAACCTT GGTAACACTT GTTACATGAA TGCCACAGTT 
CAGTGTATTC GTTCTGTGCC TGAACTCAAA GATGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCGCAG TATATTACTG CAGCCCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCCAGTATT CCACCTATTA TTCTACTGCA GTTTTTGCAC 
ATGGCTTTCC CACAGTTTGC CGAGAAAGGT GAACAAGGAC AGTATCTTCA ACAGGATGCT 
AATGAATGTT GGATACAAAT GATGCGAGTA TTGCAACAGA AATTGGAAGC AATAGAGGAT 
GATTCTGTTA AAGAGACAGA CTCCTCATCT GCATCGGCAG CGACACCTTC TAAAAAGAAA 
AGTTTAATCG ATCAGTTCTT CGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AAGTCACCAA AGGAAAGGAA AATCAACTTC AGCTTAGCTG TTTTATCAAT 
CAGGAAGTCA AGTATCTTTT TACAGGACTT AAATTGCGAC TTCAGGAAGA AATCACCAAA 
CAGTCTCCAA CGTTGCAAAG AAATGCCTTG TATATCAAAT CTTCCAAGAT CAGCCGGCTG 
CCTGCTTACT TGACCATTCA GATGGTTCGA TTTTTTTATA AAGAGAAGGA ATCTGTGAAT 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTTATGTTGG ATATGTATGA ACTGTGTACA 
CCAGAACTTC AAGAGAAAAT GGTGTCTTTT CGATCCAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAGCCAAA TACAAGTGAC AAAAAGAGTA GTCCCCAGAA AGAAGTTAAG 
TATGAACCCT TTTCTTTTGC TGATGATATT GGCTCCAATA ATTGTGGATA CTATGACTTA 
CAAGCAGTAC TAACACACCA GGGAAGGTCT AGTTCTTCAG GTCATTATGT ATCATGGGTG 
AAAAGGAAAC AAGATGAATG GATTAAGTTT GATGATGACA AAGTCAGCAT CGTAACACCA 
GAAGATATCT TACGGCTTTC TGGTGGTGGA GACTGGCATA TCGCTTACGT TCTACTCTAT 
GGGCCTCGCA GAGTTGAAAT AATGGAAGAG GAAAGTGAAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005142.1
CDS	217..1701
Translation	MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Target ORF information:

RefSeq Version	NM_005151.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005151.3

ORF Insert Sequence:

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATGGAAT GACTCTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCAG CCAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCATCTGCT 
ATGGAGTTAC CATGTGGATT GACAAACCTT GGTAACACTT GTTACATGAA TGCCACAGTT 
CAGTGTATTC GTTCTGTGCC TGAACTCAAA GATGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCGCAG TATATTACTG CAGCCCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCCAGTATT CCACCTATTA TTCTACTGCA GTTTTTGCAC 
ATGGCTTTCC CACAGTTTGC CGAGAAAGGT GAACAAGGAC AGTATCTTCA ACAGGATGCT 
AATGAATGTT GGATACAAAT GATGCGAGTA TTGCAACAGA AATTGGAAGC AATAGAGGAT 
GATTCTGTTA AAGAGACAGA CTCCTCATCT GCATCGGCAG CGACACCTTC TAAAAAGAAA 
AGTTTAATCG ATCAGTTCTT CGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AAGTCACCAA AGGAAAGGAA AATCAACTTC AGCTTAGCTG TTTTATCAAT 
CAGGAAGTCA AGTATCTTTT TACAGGACTT AAATTGCGAC TTCAGGAAGA AATCACCAAA 
CAGTCTCCAA CGTTGCAAAG AAATGCCTTG TATATCAAAT CTTCCAAGAT CAGCCGGCTG 
CCTGCTTACT TGACCATTCA GATGGTTCGA TTTTTTTATA AAGAGAAGGA ATCTGTGAAT 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTTATGTTGG ATATGTATGA ACTGTGTACA 
CCAGAACTTC AAGAGAAAAT GGTGTCTTTT CGATCCAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAGCCAAA TACAAGTGAC AAAAAGAGTA GTCCCCAGAA AGAAGTTAAG 
TATGAACCCT TTTCTTTTGC TGATGATATT GGCTCCAATA ATTGTGGATA CTATGACTTA 
CAAGCAGTAC TAACACACCA GGGAAGGTCT AGTTCTTCAG GTCATTATGT ATCATGGGTG 
AAAAGGAAAC AAGATGAATG GATTAAGTTT GATGATGACA AAGTCAGCAT CGTAACACCA 
GAAGATATCT TACGGCTTTC TGGTGGTGGA GACTGGCATA TCGCTTACGT TCTACTCTAT 
GGGCCTCGCA GAGTTGAAAT AATGGAAGAG GAAAGTGAAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP14 ( NM_005151.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005142.1 Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16474
Clone ID Related Accession (Same CDS sequence)	NM_005151.3 , NM_005151.4
Accession Version	NM_005151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573315200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 14 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC003556.1, CA438203.1, BU161860.1, AK098497.1 and AP000845.4. On Nov 22, 2018 this sequence version replaced NM_005151.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.225761.1, SRR1803613.242108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261601.8/ ENSP00000261601.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATGGAAT GACTCTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCAG CCAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCATCTGCT 
ATGGAGTTAC CATGTGGATT GACAAACCTT GGTAACACTT GTTACATGAA TGCCACAGTT 
CAGTGTATTC GTTCTGTGCC TGAACTCAAA GATGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCGCAG TATATTACTG CAGCCCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCCAGTATT CCACCTATTA TTCTACTGCA GTTTTTGCAC 
ATGGCTTTCC CACAGTTTGC CGAGAAAGGT GAACAAGGAC AGTATCTTCA ACAGGATGCT 
AATGAATGTT GGATACAAAT GATGCGAGTA TTGCAACAGA AATTGGAAGC AATAGAGGAT 
GATTCTGTTA AAGAGACAGA CTCCTCATCT GCATCGGCAG CGACACCTTC TAAAAAGAAA 
AGTTTAATCG ATCAGTTCTT CGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AAGTCACCAA AGGAAAGGAA AATCAACTTC AGCTTAGCTG TTTTATCAAT 
CAGGAAGTCA AGTATCTTTT TACAGGACTT AAATTGCGAC TTCAGGAAGA AATCACCAAA 
CAGTCTCCAA CGTTGCAAAG AAATGCCTTG TATATCAAAT CTTCCAAGAT CAGCCGGCTG 
CCTGCTTACT TGACCATTCA GATGGTTCGA TTTTTTTATA AAGAGAAGGA ATCTGTGAAT 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTTATGTTGG ATATGTATGA ACTGTGTACA 
CCAGAACTTC AAGAGAAAAT GGTGTCTTTT CGATCCAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAGCCAAA TACAAGTGAC AAAAAGAGTA GTCCCCAGAA AGAAGTTAAG 
TATGAACCCT TTTCTTTTGC TGATGATATT GGCTCCAATA ATTGTGGATA CTATGACTTA 
CAAGCAGTAC TAACACACCA GGGAAGGTCT AGTTCTTCAG GTCATTATGT ATCATGGGTG 
AAAAGGAAAC AAGATGAATG GATTAAGTTT GATGATGACA AAGTCAGCAT CGTAACACCA 
GAAGATATCT TACGGCTTTC TGGTGGTGGA GACTGGCATA TCGCTTACGT TCTACTCTAT 
GGGCCTCGCA GAGTTGAAAT AATGGAAGAG GAAAGTGAAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005142.1
CDS	143..1627
Translation	MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Target ORF information:

RefSeq Version	NM_005151.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005151.4

ORF Insert Sequence:

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATGGAAT GACTCTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCAG CCAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCATCTGCT 
ATGGAGTTAC CATGTGGATT GACAAACCTT GGTAACACTT GTTACATGAA TGCCACAGTT 
CAGTGTATTC GTTCTGTGCC TGAACTCAAA GATGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCGCAG TATATTACTG CAGCCCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCCAGTATT CCACCTATTA TTCTACTGCA GTTTTTGCAC 
ATGGCTTTCC CACAGTTTGC CGAGAAAGGT GAACAAGGAC AGTATCTTCA ACAGGATGCT 
AATGAATGTT GGATACAAAT GATGCGAGTA TTGCAACAGA AATTGGAAGC AATAGAGGAT 
GATTCTGTTA AAGAGACAGA CTCCTCATCT GCATCGGCAG CGACACCTTC TAAAAAGAAA 
AGTTTAATCG ATCAGTTCTT CGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AAGTCACCAA AGGAAAGGAA AATCAACTTC AGCTTAGCTG TTTTATCAAT 
CAGGAAGTCA AGTATCTTTT TACAGGACTT AAATTGCGAC TTCAGGAAGA AATCACCAAA 
CAGTCTCCAA CGTTGCAAAG AAATGCCTTG TATATCAAAT CTTCCAAGAT CAGCCGGCTG 
CCTGCTTACT TGACCATTCA GATGGTTCGA TTTTTTTATA AAGAGAAGGA ATCTGTGAAT 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTTATGTTGG ATATGTATGA ACTGTGTACA 
CCAGAACTTC AAGAGAAAAT GGTGTCTTTT CGATCCAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAGCCAAA TACAAGTGAC AAAAAGAGTA GTCCCCAGAA AGAAGTTAAG 
TATGAACCCT TTTCTTTTGC TGATGATATT GGCTCCAATA ATTGTGGATA CTATGACTTA 
CAAGCAGTAC TAACACACCA GGGAAGGTCT AGTTCTTCAG GTCATTATGT ATCATGGGTG 
AAAAGGAAAC AAGATGAATG GATTAAGTTT GATGATGACA AAGTCAGCAT CGTAACACCA 
GAAGATATCT TACGGCTTTC TGGTGGTGGA GACTGGCATA TCGCTTACGT TCTACTCTAT 
GGGCCTCGCA GAGTTGAAAT AATGGAAGAG GAAAGTGAAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP14 ( NM_001037334.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032411.1 Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16417
Clone ID Related Accession (Same CDS sequence)	NM_001037334.2 , NM_001037334.1
Accession Version	NM_001037334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573315200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 14 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717018.1, CR976282.1, BC003556.1, CA438203.1, BU161860.1, AK098497.1 and AP000845.4. On Jun 1, 2019 this sequence version replaced NM_001037334.1. Transcript Variant: This variant (2) lacks an alternate exon, compared to variant 1, but maintains the reading frame. The resulting protein (isoform b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.107973.1, SRR1803614.112339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATATGGA GTTACCATGT GGATTGACAA ACCTTGGTAA CACTTGTTAC 
ATGAATGCCA CAGTTCAGTG TATTCGTTCT GTGCCTGAAC TCAAAGATGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CGCAGTATAT TACTGCAGCC 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCCA GTATTCCACC TATTATTCTA 
CTGCAGTTTT TGCACATGGC TTTCCCACAG TTTGCCGAGA AAGGTGAACA AGGACAGTAT 
CTTCAACAGG ATGCTAATGA ATGTTGGATA CAAATGATGC GAGTATTGCA ACAGAAATTG 
GAAGCAATAG AGGATGATTC TGTTAAAGAG ACAGACTCCT CATCTGCATC GGCAGCGACA 
CCTTCTAAAA AGAAAAGTTT AATCGATCAG TTCTTCGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAAGTC ACCAAAGGAA AGGAAAATCA ACTTCAGCTT 
AGCTGTTTTA TCAATCAGGA AGTCAAGTAT CTTTTTACAG GACTTAAATT GCGACTTCAG 
GAAGAAATCA CCAAACAGTC TCCAACGTTG CAAAGAAATG CCTTGTATAT CAAATCTTCC 
AAGATCAGCC GGCTGCCTGC TTACTTGACC ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAGGAATCTG TGAATGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTTAT GTTGGATATG 
TATGAACTGT GTACACCAGA ACTTCAAGAG AAAATGGTGT CTTTTCGATC CAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAG CCAAATACAA GTGACAAAAA GAGTAGTCCC 
CAGAAAGAAG TTAAGTATGA ACCCTTTTCT TTTGCTGATG ATATTGGCTC CAATAATTGT 
GGATACTATG ACTTACAAGC AGTACTAACA CACCAGGGAA GGTCTAGTTC TTCAGGTCAT 
TATGTATCAT GGGTGAAAAG GAAACAAGAT GAATGGATTA AGTTTGATGA TGACAAAGTC 
AGCATCGTAA CACCAGAAGA TATCTTACGG CTTTCTGGTG GTGGAGACTG GCATATCGCT 
TACGTTCTAC TCTATGGGCC TCGCAGAGTT GAAATAATGG AAGAGGAAAG TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032411.1
CDS	143..1522
Translation	MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Target ORF information:

RefSeq Version	NM_001037334.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037334.2

ORF Insert Sequence:

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATATGGA GTTACCATGT GGATTGACAA ACCTTGGTAA CACTTGTTAC 
ATGAATGCCA CAGTTCAGTG TATTCGTTCT GTGCCTGAAC TCAAAGATGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CGCAGTATAT TACTGCAGCC 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCCA GTATTCCACC TATTATTCTA 
CTGCAGTTTT TGCACATGGC TTTCCCACAG TTTGCCGAGA AAGGTGAACA AGGACAGTAT 
CTTCAACAGG ATGCTAATGA ATGTTGGATA CAAATGATGC GAGTATTGCA ACAGAAATTG 
GAAGCAATAG AGGATGATTC TGTTAAAGAG ACAGACTCCT CATCTGCATC GGCAGCGACA 
CCTTCTAAAA AGAAAAGTTT AATCGATCAG TTCTTCGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAAGTC ACCAAAGGAA AGGAAAATCA ACTTCAGCTT 
AGCTGTTTTA TCAATCAGGA AGTCAAGTAT CTTTTTACAG GACTTAAATT GCGACTTCAG 
GAAGAAATCA CCAAACAGTC TCCAACGTTG CAAAGAAATG CCTTGTATAT CAAATCTTCC 
AAGATCAGCC GGCTGCCTGC TTACTTGACC ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAGGAATCTG TGAATGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTTAT GTTGGATATG 
TATGAACTGT GTACACCAGA ACTTCAAGAG AAAATGGTGT CTTTTCGATC CAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAG CCAAATACAA GTGACAAAAA GAGTAGTCCC 
CAGAAAGAAG TTAAGTATGA ACCCTTTTCT TTTGCTGATG ATATTGGCTC CAATAATTGT 
GGATACTATG ACTTACAAGC AGTACTAACA CACCAGGGAA GGTCTAGTTC TTCAGGTCAT 
TATGTATCAT GGGTGAAAAG GAAACAAGAT GAATGGATTA AGTTTGATGA TGACAAAGTC 
AGCATCGTAA CACCAGAAGA TATCTTACGG CTTTCTGGTG GTGGAGACTG GCATATCGCT 
TACGTTCTAC TCTATGGGCC TCGCAGAGTT GAAATAATGG AAGAGGAAAG TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP14 ( NM_001037334.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032411.1 Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.

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CloneID	OHu16417
Clone ID Related Accession (Same CDS sequence)	NM_001037334.2 , NM_001037334.1
Accession Version	NM_001037334.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551542400000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 14 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717018.1, CR976282.1, BC003556.1, CA438203.1, BU161860.1 and AK098497.1. Transcript Variant: This variant (2) lacks an alternate exon, compared to variant 1, but maintains the reading frame. The resulting protein (isoform b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.199117.1, SRR1660803.137937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATATGGA GTTACCATGT GGATTGACAA ACCTTGGTAA CACTTGTTAC 
ATGAATGCCA CAGTTCAGTG TATTCGTTCT GTGCCTGAAC TCAAAGATGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CGCAGTATAT TACTGCAGCC 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCCA GTATTCCACC TATTATTCTA 
CTGCAGTTTT TGCACATGGC TTTCCCACAG TTTGCCGAGA AAGGTGAACA AGGACAGTAT 
CTTCAACAGG ATGCTAATGA ATGTTGGATA CAAATGATGC GAGTATTGCA ACAGAAATTG 
GAAGCAATAG AGGATGATTC TGTTAAAGAG ACAGACTCCT CATCTGCATC GGCAGCGACA 
CCTTCTAAAA AGAAAAGTTT AATCGATCAG TTCTTCGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAAGTC ACCAAAGGAA AGGAAAATCA ACTTCAGCTT 
AGCTGTTTTA TCAATCAGGA AGTCAAGTAT CTTTTTACAG GACTTAAATT GCGACTTCAG 
GAAGAAATCA CCAAACAGTC TCCAACGTTG CAAAGAAATG CCTTGTATAT CAAATCTTCC 
AAGATCAGCC GGCTGCCTGC TTACTTGACC ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAGGAATCTG TGAATGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTTAT GTTGGATATG 
TATGAACTGT GTACACCAGA ACTTCAAGAG AAAATGGTGT CTTTTCGATC CAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAG CCAAATACAA GTGACAAAAA GAGTAGTCCC 
CAGAAAGAAG TTAAGTATGA ACCCTTTTCT TTTGCTGATG ATATTGGCTC CAATAATTGT 
GGATACTATG ACTTACAAGC AGTACTAACA CACCAGGGAA GGTCTAGTTC TTCAGGTCAT 
TATGTATCAT GGGTGAAAAG GAAACAAGAT GAATGGATTA AGTTTGATGA TGACAAAGTC 
AGCATCGTAA CACCAGAAGA TATCTTACGG CTTTCTGGTG GTGGAGACTG GCATATCGCT 
TACGTTCTAC TCTATGGGCC TCGCAGAGTT GAAATAATGG AAGAGGAAAG TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032411.1
CDS	217..1596
Translation	MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Target ORF information:

RefSeq Version	NM_001037334.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037334.1

ORF Insert Sequence:

ATGCCGCTCT ACTCCGTTAC TGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACAGATG AACCTCCAAT GGTATTCAAG GCTCAGCTGT TTGCGTTGAC TGGAGTCCAG 
CCTGCCAGAC AGAAAGTTAT GGTGAAAGGA GGAACGCTAA AGGATGATGA TTGGGGAAAC 
ATCAAAATAA AAAATATGGA GTTACCATGT GGATTGACAA ACCTTGGTAA CACTTGTTAC 
ATGAATGCCA CAGTTCAGTG TATTCGTTCT GTGCCTGAAC TCAAAGATGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CGCAGTATAT TACTGCAGCC 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCCA GTATTCCACC TATTATTCTA 
CTGCAGTTTT TGCACATGGC TTTCCCACAG TTTGCCGAGA AAGGTGAACA AGGACAGTAT 
CTTCAACAGG ATGCTAATGA ATGTTGGATA CAAATGATGC GAGTATTGCA ACAGAAATTG 
GAAGCAATAG AGGATGATTC TGTTAAAGAG ACAGACTCCT CATCTGCATC GGCAGCGACA 
CCTTCTAAAA AGAAAAGTTT AATCGATCAG TTCTTCGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAAGTC ACCAAAGGAA AGGAAAATCA ACTTCAGCTT 
AGCTGTTTTA TCAATCAGGA AGTCAAGTAT CTTTTTACAG GACTTAAATT GCGACTTCAG 
GAAGAAATCA CCAAACAGTC TCCAACGTTG CAAAGAAATG CCTTGTATAT CAAATCTTCC 
AAGATCAGCC GGCTGCCTGC TTACTTGACC ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAGGAATCTG TGAATGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTTAT GTTGGATATG 
TATGAACTGT GTACACCAGA ACTTCAAGAG AAAATGGTGT CTTTTCGATC CAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAG CCAAATACAA GTGACAAAAA GAGTAGTCCC 
CAGAAAGAAG TTAAGTATGA ACCCTTTTCT TTTGCTGATG ATATTGGCTC CAATAATTGT 
GGATACTATG ACTTACAAGC AGTACTAACA CACCAGGGAA GGTCTAGTTC TTCAGGTCAT 
TATGTATCAT GGGTGAAAAG GAAACAAGAT GAATGGATTA AGTTTGATGA TGACAAAGTC 
AGCATCGTAA CACCAGAAGA TATCTTACGG CTTTCTGGTG GTGGAGACTG GCATATCGCT 
TACGTTCTAC TCTATGGGCC TCGCAGAGTT GAAATAATGG AAGAGGAAAG TGAACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

USP14-mediated IκBα degradation exacerbates NF-κB activation and IL-1β-stimulated chondrocyte dedifferentiation.
Life sciences218147-152(2019 Feb)
Li M,Zhao J,Jia L

USP14 promotes K63-linked RIG-I deubiquitination and suppresses antiviral immune responses.
European journal of immunology49(1)42-53(2019 01)
Li H,Zhao Z,Ling J,Pan L,Zhao X,Zhu H,Yu J,Xie B,Shen J,Chen W

UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells.
Proceedings of the National Academy of Sciences of the United States of America115(50)E11642-E11650(2018 12)
Kim HT,Goldberg AL

USP14 inhibition corrects an
EMBO molecular medicine10(11)(2018 11)
Chakraborty J,von Stockum S,Marchesan E,Caicci F,Ferrari V,Rakovic A,Klein C,Antonini A,Bubacco L,Ziviani E

USP14 as a novel prognostic marker promotes cisplatin resistance via Akt/ERK signaling pathways in gastric cancer.
Cancer medicine7(11)5577-5588(2018 11)
Fu Y,Ma G,Liu G,Li B,Li H,Hao X,Liu L

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USP14 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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