usp14 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp14
Entrez Gene ID	335736
Full Name	ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Synonyms	wu:fk63d09,zgc:55949
General protein information	Preferred Names ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) Names ubiquitin carboxyl-terminal hydrolase 14 ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199973.1	NP_956267.1	ubiquitin carboxyl-terminal hydrolase 14

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP14	NP_005142.1
Canis lupus familiaris (dog)	USP14	XP_537306.2
Gallus gallus (chicken)	USP14	XP_004939812.1
Xenopus tropicalis (tropical clawed frog)	usp14	NP_001005641.1
Bos taurus (cattle)	USP14	NP_001068657.1
Caenorhabditis elegans (roundworm)	usp-14	NP_497006.1
Schizosaccharomyces pombe (fission yeast)	ubp6	NP_594117.2
Pan troglodytes (chimpanzee)	USP14	NP_001038961.1
Neurospora crassa	NCU01919	XP_965550.1
Magnaporthe oryzae (rice blast fungus)	MGG_02970	XP_003720730.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009348	XP_310014.4
Kluyveromyces lactis	KLLA0A10791g	XP_451470.1
Eremothecium gossypii	AGOS_AEL029W	NP_984832.2
Drosophila melanogaster (fruit fly)	CG5384	NP_001260321.1
Saccharomyces cerevisiae (baker's yeast)	UBP6	NP_116665.1
Arabidopsis thaliana (thale cress)	UBP7	NP_566680.2
Oryza sativa (rice)	Os01g0550100	NP_001043299.1
Macaca mulatta (Rhesus monkey)	USP14	XP_001090784.1
Mus musculus (house mouse)	Usp14	NP_067497.2
Rattus norvegicus (Norway rat)	Usp14	NP_001008302.1
Danio rerio (zebrafish)	usp14	NP_956267.1
Arabidopsis thaliana (thale cress)	UBP6	NP_564596.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Hsu CH, Lin JS, Po Lai K, Li JW, Chan TF, You MS, Tse WK, Jiang YJ
Scientific reports510673(2015 Jun)

Identification of infection- and defense-related genes via a dynamic host-pathogen interaction network using a Candida albicans-zebrafish infection model.
Kuo ZY, Chuang YJ, Chao CC, Liu FC, Lan CY, Chen BS
Journal of innate immunity5(2)137-52(2013)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03671	NM_199973.1 Latest version!	Danio rerio ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (usp14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp14 ( NM_199973.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956267.1 Danio rerio ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (usp14), mRNA.

Price & Availability↑

CloneID	ODa03671
Clone ID Related Accession (Same CDS sequence)	NM_199973.1
Accession Version	NM_199973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 14
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044553.1. On Jul 6, 2005 this sequence version replaced XM_697088.1. ##Evidence-Data-START## Transcript exon combination :: BC044553.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTAT TTACAGTGAA TGTGAAATGG GGAAAGGAGA AGTTTGATGC GGTGGAGCTG 
AACACAGAGG AGCCGCCCAT GGTGTTCAAA GCCCAGCTGT TTGCTCTTAC AGGAGTTCAG 
CCAGAGAGAC AGAAAGTCAT GGTGAAAGGC GGGACACTGA AGGATGATGA GTGGGGCAAT 
ATCAAGTTGA AAAATGGCAT GACTCTTCTG ATGATGGGCT CTGCTGATGC TCTTCCCGAG 
GAGCCTGTCG TTCGACCCAT GTTTGTAGAG GACATGACGG AGGAGCAGCT CGCATCTGCA 
ATGGAGTTGC CTTGTGGATT GACAAATCTG GGCAACACGT GCTACATGAA CGCAACAGTG 
CAGTGTTTAC GATCTGTCCC AGAGCTCAAA GGCGCTCTCA GGAGGTACTC CGGTGCCTTG 
CGGTCTTCTG GAGCTAACGC ACCTTCCCAG TACATCACAG CAGCTCTGCG TGATTTGTAT 
GAGTCCATGG ACAAGACCTC TTCAAGCATT CCACCCATCA TTCTCCTGCA GTTCCTGCAC 
ATGGCCTTCC CACAGTTTGC AGAAAAAGGA GACCAGGGCC AGTACCTCCA ACAGGATGCC 
AATGAGTGCT GGGTCCAGGT TATGCGGGTC CTTCAGCAGA AATTAGAACC TCAAGAACCA 
GAAACCCCAA TGGAGACTGA TGGCGATAGT GCAGCTGCTG CCTCCACAAA AAAGAAGAGT 
TTCATTGATC AGTATTTTGG GGTTGAGTTT GAAACTTCTA TGAAATGTAC CGAGGCGGAG 
GATGAGGAAC CGACTAAAGG CACAGAGAGC CAGCTTCAGC TCAGCTGCTT CATCAACCAG 
GAAGTCAAAT ATCTCGCCAC TGGACTGCGA TTGAGGCTAC AGGAGGACAT CACAAAGTTC 
TCTCCATCTT TACAGAGAAA TGCCCTCTAC ATCAAATCAT CCAAAATCAG CCGTCTCCCG 
GCGTACCACA CAGTTCAGAT GGTCCGGTTC TTTTATAAGG AAAAAGAGTC TGTGAATGCC 
AAAGTACTAA AGGATGTCAA GTTTCCTCTT ATGCTGGACG TGTACGAGCT GTGCACATCT 
GAGCTGCAGG AGAAGATGGT TTCTCTGAGG TCCAAGTTTA AGGAGATGGA GGACAAGAAA 
CTGGAGAAGC AACAGAAGTC TGAACCGAAT AAGAAGCCTG GTGCTCTAAA GGAGACAAAA 
TATGAACCTT TCAGTTTTCC CGATGACTTG GGCTCCAACA ACAGTGGCTA CTATGAACTG 
CAAGCTGTTC TGACGCATCA GGGCCGATCC AGCTCATCTG GCCATTATGT CGCCTGGGTT 
AAAAGGAAAG AAGACGAATG GGTGAAGTTT GACGATGATA AAGTCAGCGT GGTGACGCCA 
GAGGACATCT TGAAGCTGTC CGGAGGTGGA GACTGGCATA TAGCGTACGT CCTGCTGTAC 
GGCCCTCGAC GTCTGGAAAT GCTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956267.1
CDS	153..1622
Translation	MPVFTVNVKWGKEKFDAVELNTEEPPMVFKAQLFALTGVQPERQKVMVKGGTLKDDEWGNIKLKNGMTLLMMGSADALPEEPVVRPMFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCLRSVPELKGALRRYSGALRSSGANAPSQYITAALRDLYESMDKTSSSIPPIILLQFLHMAFPQFAEKGDQGQYLQQDANECWVQVMRVLQQKLEPQEPETPMETDGDSAAAASTKKKSFIDQYFGVEFETSMKCTEAEDEEPTKGTESQLQLSCFINQEVKYLATGLRLRLQEDITKFSPSLQRNALYIKSSKISRLPAYHTVQMVRFFYKEKESVNAKVLKDVKFPLMLDVYELCTSELQEKMVSLRSKFKEMEDKKLEKQQKSEPNKKPGALKETKYEPFSFPDDLGSNNSGYYELQAVLTHQGRSSSSGHYVAWVKRKEDEWVKFDDDKVSVVTPEDILKLSGGGDWHIAYVLLYGPRRLEMLE

Target ORF information:

RefSeq Version	NM_199973.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (usp14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199973.1

ORF Insert Sequence:

ATGCCCGTAT TTACAGTGAA TGTGAAATGG GGAAAGGAGA AGTTTGATGC GGTGGAGCTG 
AACACAGAGG AGCCGCCCAT GGTGTTCAAA GCCCAGCTGT TTGCTCTTAC AGGAGTTCAG 
CCAGAGAGAC AGAAAGTCAT GGTGAAAGGC GGGACACTGA AGGATGATGA GTGGGGCAAT 
ATCAAGTTGA AAAATGGCAT GACTCTTCTG ATGATGGGCT CTGCTGATGC TCTTCCCGAG 
GAGCCTGTCG TTCGACCCAT GTTTGTAGAG GACATGACGG AGGAGCAGCT CGCATCTGCA 
ATGGAGTTGC CTTGTGGATT GACAAATCTG GGCAACACGT GCTACATGAA CGCAACAGTG 
CAGTGTTTAC GATCTGTCCC AGAGCTCAAA GGCGCTCTCA GGAGGTACTC CGGTGCCTTG 
CGGTCTTCTG GAGCTAACGC ACCTTCCCAG TACATCACAG CAGCTCTGCG TGATTTGTAT 
GAGTCCATGG ACAAGACCTC TTCAAGCATT CCACCCATCA TTCTCCTGCA GTTCCTGCAC 
ATGGCCTTCC CACAGTTTGC AGAAAAAGGA GACCAGGGCC AGTACCTCCA ACAGGATGCC 
AATGAGTGCT GGGTCCAGGT TATGCGGGTC CTTCAGCAGA AATTAGAACC TCAAGAACCA 
GAAACCCCAA TGGAGACTGA TGGCGATAGT GCAGCTGCTG CCTCCACAAA AAAGAAGAGT 
TTCATTGATC AGTATTTTGG GGTTGAGTTT GAAACTTCTA TGAAATGTAC CGAGGCGGAG 
GATGAGGAAC CGACTAAAGG CACAGAGAGC CAGCTTCAGC TCAGCTGCTT CATCAACCAG 
GAAGTCAAAT ATCTCGCCAC TGGACTGCGA TTGAGGCTAC AGGAGGACAT CACAAAGTTC 
TCTCCATCTT TACAGAGAAA TGCCCTCTAC ATCAAATCAT CCAAAATCAG CCGTCTCCCG 
GCGTACCACA CAGTTCAGAT GGTCCGGTTC TTTTATAAGG AAAAAGAGTC TGTGAATGCC 
AAAGTACTAA AGGATGTCAA GTTTCCTCTT ATGCTGGACG TGTACGAGCT GTGCACATCT 
GAGCTGCAGG AGAAGATGGT TTCTCTGAGG TCCAAGTTTA AGGAGATGGA GGACAAGAAA 
CTGGAGAAGC AACAGAAGTC TGAACCGAAT AAGAAGCCTG GTGCTCTAAA GGAGACAAAA 
TATGAACCTT TCAGTTTTCC CGATGACTTG GGCTCCAACA ACAGTGGCTA CTATGAACTG 
CAAGCTGTTC TGACGCATCA GGGCCGATCC AGCTCATCTG GCCATTATGT CGCCTGGGTT 
AAAAGGAAAG AAGACGAATG GGTGAAGTTT GACGATGATA AAGTCAGCGT GGTGACGCCA 
GAGGACATCT TGAAGCTGTC CGGAGGTGGA GACTGGCATA TAGCGTACGT CCTGCTGTAC 
GGCCCTCGAC GTCTGGAAAT GCTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Scientific reports510673(2015 Jun)
Hsu CH,Lin JS,Po Lai K,Li JW,Chan TF,You MS,Tse WK,Jiang YJ

Identification of infection- and defense-related genes via a dynamic host-pathogen interaction network using a Candida albicans-zebrafish infection model.
Journal of innate immunity5(2)137-52(2013)
Kuo ZY,Chuang YJ,Chao CC,Liu FC,Lan CY,Chen BS

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ