VAMP4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAMP4
Entrez Gene ID	8674
Full Name	vesicle associated membrane protein 4
Synonyms	VAMP-4,VAMP24
General protein information	Preferred Names vesicle associated membrane protein 4 Names vesicle-associated membrane protein 4 VAMP4/TAF3 fusion
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q24.3
Summary	Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008].
Disorder MIM:	606909

mRNA and Protein(s)

mRNA	Protein	Name
XM_017002713.2	XP_016858202.1	vesicle-associated membrane protein 4 isoform X1
NM_003762.4	NP_003753.2	vesicle-associated membrane protein 4 isoform 1
NM_003762.5	NP_003753.2	vesicle-associated membrane protein 4 isoform 1
NM_001185127.1	NP_001172056.1	vesicle-associated membrane protein 4 isoform 2
NM_001185127.2	NP_001172056.1	vesicle-associated membrane protein 4 isoform 2
XM_017002713.1	XP_016858202.1	vesicle-associated membrane protein 4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VAMP4	NP_001069906.1
Rattus norvegicus (Norway rat)	Vamp4	NP_001102326.1
Xenopus tropicalis (tropical clawed frog)	vamp4	NP_001016897.1
Mus musculus (house mouse)	Vamp4	NP_058076.2
Gallus gallus (chicken)	VAMP4	XP_001233852.2
Homo sapiens (human)	VAMP4	NP_003753.2
Pan troglodytes (chimpanzee)	VAMP4	XP_003308651.1
Danio rerio (zebrafish)	vamp4	NP_957029.2

Gene Ontology
Bibliography

Related articles in PubMed

BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.
Zhang X, Jiang S, Mitok KA, Li L, Attie AD, Martin TFJ
The Journal of cell biology216(7)2151-2166(2017 07)

The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs).
Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan JC, Nunez M, Moncion T, Loew D, Formstecher E, Galli T, Proux-Gillardeaux V
The Journal of biological chemistry290(47)28056-69(2015 Nov)

O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation.
Guo B, Liang Q, Li L, Hu Z, Wu F, Zhang P, Ma Y, Zhao B, Kovács AL, Zhang Z, Feng D, Chen S, Zhang H
Nature cell biology16(12)1215-26(2014 Dec)

Vesicle-associated membrane protein 4 and syntaxin 6 interactions at the chlamydial inclusion.
Kabeiseman EJ, Cichos K, Hackstadt T, Lucas A, Moore ER
Infection and immunity81(9)3326-37(2013 Sep)

Uncoupling the functions of CALM in VAMP sorting and clathrin-coated pit formation.
Sahlender DA, Kozik P, Miller SE, Peden AA, Robinson MS
PloS one8(5)e64514(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VAMP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAMP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu90742	XM_017002713.2 Latest version!	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu15008	NM_001185127.2 Latest version!	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu15181	NM_003762.5 Latest version!	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu15181	NM_003762.4	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu15008	NM_001185127.1	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu90742	XM_017002713.1	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAMP4 ( XM_017002713.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858202.1 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90742
Clone ID Related Accession (Same CDS sequence)	XM_017002713.2 , XM_017002713.1
Accession Version	XM_017002713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGA AAGCTTATCG GATAATGCAA 
CAGCTTTTAG CAACAGATCC AAACAACTTC GAAGGCAAAT GTGGTGGCGT GGATGCAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858202.1
CDS	985..1287
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKKAYRIMQQLLATDPNNFEGKCGGVDAK

Target ORF information:

RefSeq Version	XM_017002713.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002713.2

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGA AAGCTTATCG GATAATGCAA 
CAGCTTTTAG CAACAGATCC AAACAACTTC GAAGGCAAAT GTGGTGGCGT GGATGCAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP4 ( XM_017002713.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858202.1 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90742
Clone ID Related Accession (Same CDS sequence)	XM_017002713.2 , XM_017002713.1
Accession Version	XM_017002713.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGA AAGCTTATCG GATAATGCAA 
CAGCTTTTAG CAACAGATCC AAACAACTTC GAAGGCAAAT GTGGTGGCGT GGATGCAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858202.1
CDS	380..682
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKKAYRIMQQLLATDPNNFEGKCGGVDAK

Target ORF information:

RefSeq Version	XM_017002713.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002713.1

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGA AAGCTTATCG GATAATGCAA 
CAGCTTTTAG CAACAGATCC AAACAACTTC GAAGGCAAAT GTGGTGGCGT GGATGCAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP4 ( NM_001185127.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172056.1 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15008
Clone ID Related Accession (Same CDS sequence)	NM_001185127.1 , NM_001185127.2
Accession Version	NM_001185127.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB167275.1, BC007019.1 and Z98751.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007019.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTGGGACCA 
TCTGGACCAA GATTTGGACC TAGAAATGAT AAAATTAAGC ATGTTCAGAA TCAAGTGGAT 
GAAGTTATTG ATGTCATGCA AGAAAATATT ACAAAGGTAA TTGAGAGAGG GGAGAGACTA 
GATGAACTAC AGGACAAATC AGAAAGCTTA TCGGATAATG CAACAGCTTT TAGCAACAGA 
TCCAAACAAC TTCGAAGGCA AATGTGGTGG CGTGGATGCA AAATAAAAGC CATCATGGCT 
TTGGTTGCTG CTATCCTTTT GCTAGTGATT ATCATTCTTA TAGTCATGAA ATACCGTACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172056.1
CDS	380..802
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

Target ORF information:

RefSeq Version	NM_001185127.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185127.1

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTGGGACCA 
TCTGGACCAA GATTTGGACC TAGAAATGAT AAAATTAAGC ATGTTCAGAA TCAAGTGGAT 
GAAGTTATTG ATGTCATGCA AGAAAATATT ACAAAGGTAA TTGAGAGAGG GGAGAGACTA 
GATGAACTAC AGGACAAATC AGAAAGCTTA TCGGATAATG CAACAGCTTT TAGCAACAGA 
TCCAAACAAC TTCGAAGGCA AATGTGGTGG CGTGGATGCA AAATAAAAGC CATCATGGCT 
TTGGTTGCTG CTATCCTTTT GCTAGTGATT ATCATTCTTA TAGTCATGAA ATACCGTACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP4 ( NM_001185127.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172056.1 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15008
Clone ID Related Accession (Same CDS sequence)	NM_001185127.1 , NM_001185127.2
Accession Version	NM_001185127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007019.1 and Z98751.1. On May 31, 2019 this sequence version replaced NM_001185127.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007019.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTGGGACCA 
TCTGGACCAA GATTTGGACC TAGAAATGAT AAAATTAAGC ATGTTCAGAA TCAAGTGGAT 
GAAGTTATTG ATGTCATGCA AGAAAATATT ACAAAGGTAA TTGAGAGAGG GGAGAGACTA 
GATGAACTAC AGGACAAATC AGAAAGCTTA TCGGATAATG CAACAGCTTT TAGCAACAGA 
TCCAAACAAC TTCGAAGGCA AATGTGGTGG CGTGGATGCA AAATAAAAGC CATCATGGCT 
TTGGTTGCTG CTATCCTTTT GCTAGTGATT ATCATTCTTA TAGTCATGAA ATACCGTACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172056.1
CDS	206..628
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

Target ORF information:

RefSeq Version	NM_001185127.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185127.2

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTGGGACCA 
TCTGGACCAA GATTTGGACC TAGAAATGAT AAAATTAAGC ATGTTCAGAA TCAAGTGGAT 
GAAGTTATTG ATGTCATGCA AGAAAATATT ACAAAGGTAA TTGAGAGAGG GGAGAGACTA 
GATGAACTAC AGGACAAATC AGAAAGCTTA TCGGATAATG CAACAGCTTT TAGCAACAGA 
TCCAAACAAC TTCGAAGGCA AATGTGGTGG CGTGGATGCA AAATAAAAGC CATCATGGCT 
TTGGTTGCTG CTATCCTTTT GCTAGTGATT ATCATTCTTA TAGTCATGAA ATACCGTACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP4 ( NM_003762.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003753.2 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15181
Clone ID Related Accession (Same CDS sequence)	NM_003762.4 , NM_003762.5
Accession Version	NM_003762.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB167275.1, AL035296.1 and Z98751.1. On Jun 5, 2010 this sequence version replaced NM_003762.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.6601.1, SRR1660803.131920.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGG TAATTGAGAG AGGGGAGAGA 
CTAGATGAAC TACAGGACAA ATCAGAAAGC TTATCGGATA ATGCAACAGC TTTTAGCAAC 
AGATCCAAAC AACTTCGAAG GCAAATGTGG TGGCGTGGAT GCAAAATAAA AGCCATCATG 
GCTTTGGTTG CTGCTATCCT TTTGCTAGTG ATTATCATTC TTATAGTCAT GAAATACCGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003753.2
CDS	380..805
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

Target ORF information:

RefSeq Version	NM_003762.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003762.4

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGG TAATTGAGAG AGGGGAGAGA 
CTAGATGAAC TACAGGACAA ATCAGAAAGC TTATCGGATA ATGCAACAGC TTTTAGCAAC 
AGATCCAAAC AACTTCGAAG GCAAATGTGG TGGCGTGGAT GCAAAATAAA AGCCATCATG 
GCTTTGGTTG CTGCTATCCT TTTGCTAGTG ATTATCATTC TTATAGTCAT GAAATACCGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP4 ( NM_003762.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003753.2 Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15181
Clone ID Related Accession (Same CDS sequence)	NM_003762.4 , NM_003762.5
Accession Version	NM_003762.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB167275.1, AL035296.1 and Z98751.1. On Nov 23, 2018 this sequence version replaced NM_003762.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.517.1, SRR1660803.6601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000236192.12/ ENSP00000236192.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGG TAATTGAGAG AGGGGAGAGA 
CTAGATGAAC TACAGGACAA ATCAGAAAGC TTATCGGATA ATGCAACAGC TTTTAGCAAC 
AGATCCAAAC AACTTCGAAG GCAAATGTGG TGGCGTGGAT GCAAAATAAA AGCCATCATG 
GCTTTGGTTG CTGCTATCCT TTTGCTAGTG ATTATCATTC TTATAGTCAT GAAATACCGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003753.2
CDS	206..631
Translation	MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

Target ORF information:

RefSeq Version	NM_003762.5
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 4 (VAMP4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003762.5

ORF Insert Sequence:

ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG TCACAGGTTC TGTGAAAAGT 
GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG AGGACTTTTT TCTAAGGGGA 
CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA AGCATGTTCA GAATCAAGTG 
GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGG TAATTGAGAG AGGGGAGAGA 
CTAGATGAAC TACAGGACAA ATCAGAAAGC TTATCGGATA ATGCAACAGC TTTTAGCAAC 
AGATCCAAAC AACTTCGAAG GCAAATGTGG TGGCGTGGAT GCAAAATAAA AGCCATCATG 
GCTTTGGTTG CTGCTATCCT TTTGCTAGTG ATTATCATTC TTATAGTCAT GAAATACCGT 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.
The Journal of cell biology216(7)2151-2166(2017 07)
Zhang X,Jiang S,Mitok KA,Li L,Attie AD,Martin TFJ

The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs).
The Journal of biological chemistry290(47)28056-69(2015 Nov)
Kuster A,Nola S,Dingli F,Vacca B,Gauchy C,Beaujouan JC,Nunez M,Moncion T,Loew D,Formstecher E,Galli T,Proux-Gillardeaux V

O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation.
Nature cell biology16(12)1215-26(2014 Dec)
Guo B,Liang Q,Li L,Hu Z,Wu F,Zhang P,Ma Y,Zhao B,Kovács AL,Zhang Z,Feng D,Chen S,Zhang H

Vesicle-associated membrane protein 4 and syntaxin 6 interactions at the chlamydial inclusion.
Infection and immunity81(9)3326-37(2013 Sep)
Kabeiseman EJ,Cichos K,Hackstadt T,Lucas A,Moore ER

Uncoupling the functions of CALM in VAMP sorting and clathrin-coated pit formation.
PloS one8(5)e64514(2013)
Sahlender DA,Kozik P,Miller SE,Peden AA,Robinson MS

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VAMP4 cDNA ORF clone, Homo sapiens(human)

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