NKD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NKD1
Entrez Gene ID	85407
Full Name	naked cuticle homolog 1
Synonyms	Naked1
General protein information	Preferred Names naked cuticle homolog 1 Names protein naked cuticle homolog 1 Dvl-binding protein naked cuticle-1 naked-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q12.1
Summary	In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.[supplied by OMIM, Jun 2003].
Disorder MIM:	607851

mRNA and Protein(s)

mRNA	Protein	Name
XM_017023804.1	XP_016879293.1	protein naked cuticle homolog 1 isoform X1
NM_033119.4	NP_149110.1	protein naked cuticle homolog 1
XM_011523399.2	XP_011521701.1	protein naked cuticle homolog 1 isoform X1
NM_033119.5	NP_149110.1	protein naked cuticle homolog 1
XM_011523400.2	XP_011521702.1	protein naked cuticle homolog 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NKD1	NP_001098923.1
Mus musculus (house mouse)	Nkd1	NP_081556.3
Rattus norvegicus (Norway rat)	Nkd1	NP_001258310.1
Xenopus tropicalis (tropical clawed frog)	nkd1	NP_001016798.1
Canis lupus familiaris (dog)	NKD1	XP_544414.3
Danio rerio (zebrafish)	nkd1	NP_001036798.1
Homo sapiens (human)	NKD1	NP_149110.1
Pan troglodytes (chimpanzee)	NKD1	XP_520634.2
Gallus gallus (chicken)	NKD1	XP_414094.3
Macaca mulatta (Rhesus monkey)	NKD1	XP_001086556.2

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of NKD1 in human osteosarcoma and its clinical significance.
Chen X, Xu P, Zhu J, Liu F
Molecular medicine reports17(1)1111-1117(2018 Jan)

Long non-coding RNA HNF1A-AS1 promotes hepatocellular carcinoma cell proliferation by repressing NKD1 and P21 expression.
Wang C, Mou L, Chai HX, Wang F, Yin YZ, Zhang XY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie89926-932(2017 May)

Low NKD1 expression predicts adverse prognosis in cytogenetically normal acute myeloid leukemia.
Zhou JD, Yao DM, Han L, Xiao GF, Guo H, Zhang TJ, Li XX, Yuan Q, Yang L, Lin J, Qian J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(4)1010428317699123(2017 Apr)

MicroRNA-195-5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1.
Qu Q, Chu X, Wang P
Cell biology international41(3)287-295(2017 Mar)

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.
Guan H, Liu C, Fang F, Huang Y, Tao T, Ling Z, You Z, Han X, Chen S, Xu B, Chen M
Oncotarget8(9)14693-14707(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NKD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu73959	XM_011523399.2 Latest version!	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu73959	XM_017023804.1 Latest version!	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu73959	XM_011523400.2 Latest version!	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu00864	NM_033119.5 Latest version!	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu00864	NM_033119.4	Homo sapiens naked cuticle homolog 1 (NKD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NKD1 ( XM_017023804.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879293.1 Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73959
Clone ID Related Accession (Same CDS sequence)	XM_017023804.1 , XM_011523399.2 , XM_011523400.2
Accession Version	XM_017023804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein naked cuticle homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879293.1
CDS	1968..3167
Translation	MVGQVVYCTRISSQAVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Target ORF information:

RefSeq Version	XM_017023804.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023804.1

ORF Insert Sequence:

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKD1 ( XM_011523399.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521701.1 Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu73959
Clone ID Related Accession (Same CDS sequence)	XM_017023804.1 , XM_011523399.2 , XM_011523400.2
Accession Version	XM_011523399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein naked cuticle homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521701.1
CDS	749..1948
Translation	MVGQVVYCTRISSQAVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Target ORF information:

RefSeq Version	XM_011523399.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523399.2

ORF Insert Sequence:

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKD1 ( XM_011523400.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521702.1 Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu73959
Clone ID Related Accession (Same CDS sequence)	XM_017023804.1 , XM_011523399.2 , XM_011523400.2
Accession Version	XM_011523400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein naked cuticle homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521702.1
CDS	40..1239
Translation	MVGQVVYCTRISSQAVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Target ORF information:

RefSeq Version	XM_011523400.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523400.2

ORF Insert Sequence:

ATGGTTGGGC AGGTTGTGTA CTGCACAAGG ATTTCCAGCC AAGCAGTGGC CCTGCCTCCT 
GAGAAGACTG ACGGGCTGGG CAGCGGAGAT GAGAAGAAGA TGGAGAGAGT GAGCGAACCC 
TGCCCAGGCT CCAAGAAGCA GCTGAAGTTT GAAGAGCTCC AGTGCGACGT GTCCATGGAG 
GAGGACAGCC GGCAGGAGTG GACCTTCACC CTGTATGACT TTGACAACAA CGGCAAGGTC 
ACCCGAGAGG ACATCACCAG CTTGCTGCAC ACCATCTATG AGGTGGTGGA CTCCTCTGTC 
AACCACTCCC CAACATCCAG CAAGATGCTG CGGGTAAAGC TCACCGTGGC CCCCGATGGC 
AGCCAGAGCA AGAGGAGCGT CCTTGTCAAT CAGGCTGACC TGCAGAGCGC AAGGCCCCGA 
GCAGAGACCA AGCCCACTGA GGACCTGCGG AGCTGGGAGA AGAAGCAGCG AGCCCCGCTC 
AGGTTCCAGG GTGACAGCCG CCTGGAGCAG TCTGGCTGCT ACCACCATTG CGTAGATGAG 
AACATCGAGA GGAGAAACCA CTACTTAGAT CTCGCCGGGA TAGAAAACTA CACGTCCCAA 
TTTGGGCCTG GCTCCCCTTC CGTGGCCCAG AAGTCAGAAC TGCCCCCCCG CACCTCCAAT 
CCCACTCGAT CTCGCTCCCA TGAGCCGGAA GCCATCCACA TCCCACACCG AAAGCCCCAA 
GGCGTGGACC CGGCCTCCTT CCACTTCCTT GACACCCCAA TCGCCAAGGT CTCAGAGCTC 
CAGCAACGGC TCCGGGGCAC CCAGGACGGG AGCAAGCACT TTGTGAGGTC CCCCAAGGCC 
CAGGGCAAGA GTGTGGGTGT GGGCCACGTG GCCAGAGGGG CAAGAAACAA GCCCCCTCTG 
GGACCCGCCA TCCCTGCGGT GTCCCCCTCC GCCCACCTGG CTGCCAGCCC GGCCCTCCTC 
CCCTCCCTAG CCCCCCTCGG GCACAAGAAG CACAAGCACC GAGCCAAGGA GAGCCAGCAG 
GGCTGCCGGG GCCTGCAGGC ACCACTGGCC TCAGGTGGCC CTGTCCTGGG GCGGGAGCAC 
CTGCGGGAGC TGCCCGCCTT GGTGGTGTAT GAGAGCCAGG CCGGGCAGCC GGTCCAGAGA 
CATGAGCACC ACCACCACCA TGAACATCAC CACCATTACC ACCACTTCTA CCAGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKD1 ( NM_033119.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_149110.1 Homo sapiens naked cuticle homolog 1 (NKD1), mRNA.

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CloneID	OHu00864
Clone ID Related Accession (Same CDS sequence)	NM_033119.4 , NM_033119.5
Accession Version	NM_033119.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	protein naked cuticle homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC051288.1 and AC007608.7. On Jun 21, 2011 this sequence version replaced NM_033119.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051288.1, AF289584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAAC TTCACTCCAA GCCGGCCGCC GTGTGCAAGC GCAGGGAGAG CCCGGAAGGT 
GACAGCTTCG CCGTGAGCGC TGCCTGGGCT CGGAAGGGCA TCGAGGAGTG GATCGGGAGA 
CAGCGCTGCC CGGGCGGTGT CTCGGGACCC CGACAGCTGC GGTTGGCGGG CACCATAGGC 
CGAAGCACCC GGGAGCTCGT GGGCGACGTG TTGAGAGACA CGCTCAGCGA GGAAGAGGAG 
GACGACTTTC GGCTGGAAGT GGCCCTGCCT CCTGAGAAGA CTGACGGGCT GGGCAGCGGA 
GATGAGAAGA AGATGGAGAG AGTGAGCGAA CCCTGCCCAG GCTCCAAGAA GCAGCTGAAG 
TTTGAAGAGC TCCAGTGCGA CGTGTCCATG GAGGAGGACA GCCGGCAGGA GTGGACCTTC 
ACCCTGTATG ACTTTGACAA CAACGGCAAG GTCACCCGAG AGGACATCAC CAGCTTGCTG 
CACACCATCT ATGAGGTGGT GGACTCCTCT GTCAACCACT CCCCAACATC CAGCAAGATG 
CTGCGGGTAA AGCTCACCGT GGCCCCCGAT GGCAGCCAGA GCAAGAGGAG CGTCCTTGTC 
AATCAGGCTG ACCTGCAGAG CGCAAGGCCC CGAGCAGAGA CCAAGCCCAC TGAGGACCTG 
CGGAGCTGGG AGAAGAAGCA GCGAGCCCCG CTCAGGTTCC AGGGTGACAG CCGCCTGGAG 
CAGTCTGGCT GCTACCACCA TTGCGTAGAT GAGAACATCG AGAGGAGAAA CCACTACTTA 
GATCTCGCCG GGATAGAAAA CTACACGTCC CAATTTGGGC CTGGCTCCCC TTCCGTGGCC 
CAGAAGTCAG AACTGCCCCC CCGCACCTCC AATCCCACTC GATCTCGCTC CCATGAGCCG 
GAAGCCATCC ACATCCCACA CCGAAAGCCC CAAGGCGTGG ACCCGGCCTC CTTCCACTTC 
CTTGACACCC CAATCGCCAA GGTCTCAGAG CTCCAGCAAC GGCTCCGGGG CACCCAGGAC 
GGGAGCAAGC ACTTTGTGAG GTCCCCCAAG GCCCAGGGCA AGAGTGTGGG TGTGGGCCAC 
GTGGCCAGAG GGGCAAGAAA CAAGCCCCCT CTGGGACCCG CCATCCCTGC GGTGTCCCCC 
TCCGCCCACC TGGCTGCCAG CCCGGCCCTC CTCCCCTCCC TAGCCCCCCT CGGGCACAAG 
AAGCACAAGC ACCGAGCCAA GGAGAGCCAG CAGGGCTGCC GGGGCCTGCA GGCACCACTG 
GCCTCAGGTG GCCCTGTCCT GGGGCGGGAG CACCTGCGGG AGCTGCCCGC CTTGGTGGTG 
TATGAGAGCC AGGCCGGGCA GCCGGTCCAG AGACATGAGC ACCACCACCA CCATGAACAT 
CACCACCATT ACCACCACTT CTACCAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149110.1
CDS	225..1637
Translation	MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGGVSGPRQLRLAGTIGRSTRELVGDVLRDTLSEEEEDDFRLEVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Target ORF information:

RefSeq Version	NM_033119.4
Organism	Homo sapiens(human)
Definition	Homo sapiens naked cuticle homolog 1 (NKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033119.4

ORF Insert Sequence:

ATGGGGAAAC TTCACTCCAA GCCGGCCGCC GTGTGCAAGC GCAGGGAGAG CCCGGAAGGT 
GACAGCTTCG CCGTGAGCGC TGCCTGGGCT CGGAAGGGCA TCGAGGAGTG GATCGGGAGA 
CAGCGCTGCC CGGGCGGTGT CTCGGGACCC CGACAGCTGC GGTTGGCGGG CACCATAGGC 
CGAAGCACCC GGGAGCTCGT GGGCGACGTG TTGAGAGACA CGCTCAGCGA GGAAGAGGAG 
GACGACTTTC GGCTGGAAGT GGCCCTGCCT CCTGAGAAGA CTGACGGGCT GGGCAGCGGA 
GATGAGAAGA AGATGGAGAG AGTGAGCGAA CCCTGCCCAG GCTCCAAGAA GCAGCTGAAG 
TTTGAAGAGC TCCAGTGCGA CGTGTCCATG GAGGAGGACA GCCGGCAGGA GTGGACCTTC 
ACCCTGTATG ACTTTGACAA CAACGGCAAG GTCACCCGAG AGGACATCAC CAGCTTGCTG 
CACACCATCT ATGAGGTGGT GGACTCCTCT GTCAACCACT CCCCAACATC CAGCAAGATG 
CTGCGGGTAA AGCTCACCGT GGCCCCCGAT GGCAGCCAGA GCAAGAGGAG CGTCCTTGTC 
AATCAGGCTG ACCTGCAGAG CGCAAGGCCC CGAGCAGAGA CCAAGCCCAC TGAGGACCTG 
CGGAGCTGGG AGAAGAAGCA GCGAGCCCCG CTCAGGTTCC AGGGTGACAG CCGCCTGGAG 
CAGTCTGGCT GCTACCACCA TTGCGTAGAT GAGAACATCG AGAGGAGAAA CCACTACTTA 
GATCTCGCCG GGATAGAAAA CTACACGTCC CAATTTGGGC CTGGCTCCCC TTCCGTGGCC 
CAGAAGTCAG AACTGCCCCC CCGCACCTCC AATCCCACTC GATCTCGCTC CCATGAGCCG 
GAAGCCATCC ACATCCCACA CCGAAAGCCC CAAGGCGTGG ACCCGGCCTC CTTCCACTTC 
CTTGACACCC CAATCGCCAA GGTCTCAGAG CTCCAGCAAC GGCTCCGGGG CACCCAGGAC 
GGGAGCAAGC ACTTTGTGAG GTCCCCCAAG GCCCAGGGCA AGAGTGTGGG TGTGGGCCAC 
GTGGCCAGAG GGGCAAGAAA CAAGCCCCCT CTGGGACCCG CCATCCCTGC GGTGTCCCCC 
TCCGCCCACC TGGCTGCCAG CCCGGCCCTC CTCCCCTCCC TAGCCCCCCT CGGGCACAAG 
AAGCACAAGC ACCGAGCCAA GGAGAGCCAG CAGGGCTGCC GGGGCCTGCA GGCACCACTG 
GCCTCAGGTG GCCCTGTCCT GGGGCGGGAG CACCTGCGGG AGCTGCCCGC CTTGGTGGTG 
TATGAGAGCC AGGCCGGGCA GCCGGTCCAG AGACATGAGC ACCACCACCA CCATGAACAT 
CACCACCATT ACCACCACTT CTACCAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKD1 ( NM_033119.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_149110.1 Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), mRNA.

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CloneID	OHu00864
Clone ID Related Accession (Same CDS sequence)	NM_033119.4 , NM_033119.5
Accession Version	NM_033119.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556640000000
Organism	Homo sapiens(human)
Product	protein naked cuticle homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC051288.1 and AC007608.7. On Nov 22, 2018 this sequence version replaced NM_033119.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY061883.1, BC051288.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000268459.6/ ENSP00000268459.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAAC TTCACTCCAA GCCGGCCGCC GTGTGCAAGC GCAGGGAGAG CCCGGAAGGT 
GACAGCTTCG CCGTGAGCGC TGCCTGGGCT CGGAAGGGCA TCGAGGAGTG GATCGGGAGA 
CAGCGCTGCC CGGGCGGTGT CTCGGGACCC CGACAGCTGC GGTTGGCGGG CACCATAGGC 
CGAAGCACCC GGGAGCTCGT GGGCGACGTG TTGAGAGACA CGCTCAGCGA GGAAGAGGAG 
GACGACTTTC GGCTGGAAGT GGCCCTGCCT CCTGAGAAGA CTGACGGGCT GGGCAGCGGA 
GATGAGAAGA AGATGGAGAG AGTGAGCGAA CCCTGCCCAG GCTCCAAGAA GCAGCTGAAG 
TTTGAAGAGC TCCAGTGCGA CGTGTCCATG GAGGAGGACA GCCGGCAGGA GTGGACCTTC 
ACCCTGTATG ACTTTGACAA CAACGGCAAG GTCACCCGAG AGGACATCAC CAGCTTGCTG 
CACACCATCT ATGAGGTGGT GGACTCCTCT GTCAACCACT CCCCAACATC CAGCAAGATG 
CTGCGGGTAA AGCTCACCGT GGCCCCCGAT GGCAGCCAGA GCAAGAGGAG CGTCCTTGTC 
AATCAGGCTG ACCTGCAGAG CGCAAGGCCC CGAGCAGAGA CCAAGCCCAC TGAGGACCTG 
CGGAGCTGGG AGAAGAAGCA GCGAGCCCCG CTCAGGTTCC AGGGTGACAG CCGCCTGGAG 
CAGTCTGGCT GCTACCACCA TTGCGTAGAT GAGAACATCG AGAGGAGAAA CCACTACTTA 
GATCTCGCCG GGATAGAAAA CTACACGTCC CAATTTGGGC CTGGCTCCCC TTCCGTGGCC 
CAGAAGTCAG AACTGCCCCC CCGCACCTCC AATCCCACTC GATCTCGCTC CCATGAGCCG 
GAAGCCATCC ACATCCCACA CCGAAAGCCC CAAGGCGTGG ACCCGGCCTC CTTCCACTTC 
CTTGACACCC CAATCGCCAA GGTCTCAGAG CTCCAGCAAC GGCTCCGGGG CACCCAGGAC 
GGGAGCAAGC ACTTTGTGAG GTCCCCCAAG GCCCAGGGCA AGAGTGTGGG TGTGGGCCAC 
GTGGCCAGAG GGGCAAGAAA CAAGCCCCCT CTGGGACCCG CCATCCCTGC GGTGTCCCCC 
TCCGCCCACC TGGCTGCCAG CCCGGCCCTC CTCCCCTCCC TAGCCCCCCT CGGGCACAAG 
AAGCACAAGC ACCGAGCCAA GGAGAGCCAG CAGGGCTGCC GGGGCCTGCA GGCACCACTG 
GCCTCAGGTG GCCCTGTCCT GGGGCGGGAG CACCTGCGGG AGCTGCCCGC CTTGGTGGTG 
TATGAGAGCC AGGCCGGGCA GCCGGTCCAG AGACATGAGC ACCACCACCA CCATGAACAT 
CACCACCATT ACCACCACTT CTACCAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149110.1
CDS	159..1571
Translation	MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGGVSGPRQLRLAGTIGRSTRELVGDVLRDTLSEEEEDDFRLEVALPPEKTDGLGSGDEKKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTEDLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLPSLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQRHEHHHHHEHHHHYHHFYQT

Target ORF information:

RefSeq Version	NM_033119.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033119.5

ORF Insert Sequence:

ATGGGGAAAC TTCACTCCAA GCCGGCCGCC GTGTGCAAGC GCAGGGAGAG CCCGGAAGGT 
GACAGCTTCG CCGTGAGCGC TGCCTGGGCT CGGAAGGGCA TCGAGGAGTG GATCGGGAGA 
CAGCGCTGCC CGGGCGGTGT CTCGGGACCC CGACAGCTGC GGTTGGCGGG CACCATAGGC 
CGAAGCACCC GGGAGCTCGT GGGCGACGTG TTGAGAGACA CGCTCAGCGA GGAAGAGGAG 
GACGACTTTC GGCTGGAAGT GGCCCTGCCT CCTGAGAAGA CTGACGGGCT GGGCAGCGGA 
GATGAGAAGA AGATGGAGAG AGTGAGCGAA CCCTGCCCAG GCTCCAAGAA GCAGCTGAAG 
TTTGAAGAGC TCCAGTGCGA CGTGTCCATG GAGGAGGACA GCCGGCAGGA GTGGACCTTC 
ACCCTGTATG ACTTTGACAA CAACGGCAAG GTCACCCGAG AGGACATCAC CAGCTTGCTG 
CACACCATCT ATGAGGTGGT GGACTCCTCT GTCAACCACT CCCCAACATC CAGCAAGATG 
CTGCGGGTAA AGCTCACCGT GGCCCCCGAT GGCAGCCAGA GCAAGAGGAG CGTCCTTGTC 
AATCAGGCTG ACCTGCAGAG CGCAAGGCCC CGAGCAGAGA CCAAGCCCAC TGAGGACCTG 
CGGAGCTGGG AGAAGAAGCA GCGAGCCCCG CTCAGGTTCC AGGGTGACAG CCGCCTGGAG 
CAGTCTGGCT GCTACCACCA TTGCGTAGAT GAGAACATCG AGAGGAGAAA CCACTACTTA 
GATCTCGCCG GGATAGAAAA CTACACGTCC CAATTTGGGC CTGGCTCCCC TTCCGTGGCC 
CAGAAGTCAG AACTGCCCCC CCGCACCTCC AATCCCACTC GATCTCGCTC CCATGAGCCG 
GAAGCCATCC ACATCCCACA CCGAAAGCCC CAAGGCGTGG ACCCGGCCTC CTTCCACTTC 
CTTGACACCC CAATCGCCAA GGTCTCAGAG CTCCAGCAAC GGCTCCGGGG CACCCAGGAC 
GGGAGCAAGC ACTTTGTGAG GTCCCCCAAG GCCCAGGGCA AGAGTGTGGG TGTGGGCCAC 
GTGGCCAGAG GGGCAAGAAA CAAGCCCCCT CTGGGACCCG CCATCCCTGC GGTGTCCCCC 
TCCGCCCACC TGGCTGCCAG CCCGGCCCTC CTCCCCTCCC TAGCCCCCCT CGGGCACAAG 
AAGCACAAGC ACCGAGCCAA GGAGAGCCAG CAGGGCTGCC GGGGCCTGCA GGCACCACTG 
GCCTCAGGTG GCCCTGTCCT GGGGCGGGAG CACCTGCGGG AGCTGCCCGC CTTGGTGGTG 
TATGAGAGCC AGGCCGGGCA GCCGGTCCAG AGACATGAGC ACCACCACCA CCATGAACAT 
CACCACCATT ACCACCACTT CTACCAGACA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of NKD1 in human osteosarcoma and its clinical significance.
Molecular medicine reports17(1)1111-1117(2018 Jan)
Chen X,Xu P,Zhu J,Liu F

Long non-coding RNA HNF1A-AS1 promotes hepatocellular carcinoma cell proliferation by repressing NKD1 and P21 expression.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie89926-932(2017 May)
Wang C,Mou L,Chai HX,Wang F,Yin YZ,Zhang XY

Low NKD1 expression predicts adverse prognosis in cytogenetically normal acute myeloid leukemia.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(4)1010428317699123(2017 Apr)
Zhou JD,Yao DM,Han L,Xiao GF,Guo H,Zhang TJ,Li XX,Yuan Q,Yang L,Lin J,Qian J

MicroRNA-195-5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1.
Cell biology international41(3)287-295(2017 Mar)
Qu Q,Chu X,Wang P

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.
Oncotarget8(9)14693-14707(2017 Feb)
Guan H,Liu C,Fang F,Huang Y,Tao T,Ling Z,You Z,Han X,Chen S,Xu B,Chen M

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NKD1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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