QTRT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	QTRT1
Entrez Gene ID	81890
Full Name	queuine tRNA-ribosyltransferase catalytic subunit 1
Synonyms	FP3235,TGT,TGUT
General protein information	Preferred Names queuine tRNA-ribosyltransferase catalytic subunit 1 Names queuine tRNA-ribosyltransferase catalytic subunit 1 TGT, 43-KD subunit TGT, catalytic subunit guanine insertion enzyme queuine tRNA-ribosyltransferase 1 tRNA-guanine transglycosylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012].
Disorder MIM:	609615

mRNA and Protein(s)

mRNA	Protein	Name
NM_031209.2	NP_112486.1	queuine tRNA-ribosyltransferase catalytic subunit 1
NM_031209.3	NP_112486.1	queuine tRNA-ribosyltransferase catalytic subunit 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os09g0469900	NP_001063434.1
Neurospora crassa	NCU07015	XP_001728151.1
Magnaporthe oryzae (rice blast fungus)	MGG_04110	XP_003719721.1
Bos taurus (cattle)	QTRT1	NP_001193621.1
Drosophila melanogaster (fruit fly)	Tgt	NP_608585.1
Xenopus tropicalis (tropical clawed frog)	qtrt1	NP_989090.1
Pan troglodytes (chimpanzee)	QTRT1	XP_512381.1
Mus musculus (house mouse)	Qtrt1	NP_068688.2
Rattus norvegicus (Norway rat)	Qtrt1	NP_071586.2
Homo sapiens (human)	QTRT1	NP_112486.1
Danio rerio (zebrafish)	qtrt1	NP_957304.1
Caenorhabditis elegans (roundworm)	tgt-1	NP_502268.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003622	XP_313382.3
Schizosaccharomyces pombe (fission yeast)	SPAC1687.19c	NP_593138.1
Canis lupus familiaris (dog)	QTRT1	XP_854015.1

Gene Ontology
Bibliography

Related articles in PubMed

Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor.
Biela I, Tidten-Luksch N, Immekus F, Glinca S, Nguyen TX, Gerber HD, Heine A, Klebe G, Reuter K
PloS one8(5)e64240(2013)

Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases.
Eric Thomas C, Chen YC, Garcia GA
Biochemical and biophysical research communications410(1)34-9(2011 Jun)

Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases.
Chen YC, Brooks AF, Goodenough-Lashua DM, Kittendorf JD, Showalter HD, Garcia GA
Nucleic acids research39(7)2834-44(2011 Apr)

Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure.
Chen YC, Kelly VP, Stachura SV, Garcia GA
RNA (New York, N.Y.)16(5)958-68(2010 May)

Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit.
Deshpande KL, Katze JR
Gene265(1-2)205-12(2001 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following QTRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the QTRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31882	NM_031209.3 Latest version!	Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$237.30 $339.00
View Old Accession Versions >>
OHu31882	NM_031209.2	Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

QTRT1 ( NM_031209.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_112486.1 Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.

Price & Availability↑

CloneID	OHu31882
Clone ID Related Accession (Same CDS sequence)	NM_031209.2 , NM_031209.3
Accession Version	NM_031209.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	queuine tRNA-ribosyltransferase catalytic subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT003728.1, AF302783.1 and AF370379.1. On Jan 23, 2009 this sequence version replaced NM_031209.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.159676.1, SRR1163655.415021.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG 
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA 
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG 
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT 
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG 
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG 
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC 
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC 
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT 
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA 
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG 
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG 
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC 
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG 
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC 
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC 
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC 
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG 
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC 
ACACTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112486.1
CDS	26..1237
Translation	MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWATDALASVGITLG

Target ORF information:

RefSeq Version	NM_031209.2
Organism	Homo sapiens(human)
Definition	Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031209.2

ORF Insert Sequence:

ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG 
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA 
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG 
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT 
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG 
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG 
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC 
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC 
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT 
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA 
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG 
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG 
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC 
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG 
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC 
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC 
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC 
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG 
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC 
ACACTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QTRT1 ( NM_031209.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_112486.1 Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.

Price & Availability↑

CloneID	OHu31882
Clone ID Related Accession (Same CDS sequence)	NM_031209.2 , NM_031209.3
Accession Version	NM_031209.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	queuine tRNA-ribosyltransferase catalytic subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT003728.1, AF302783.1 and AF370379.1. On Nov 23, 2018 this sequence version replaced NM_031209.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.657272.1, AF302783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000250237.10/ ENSP00000250237.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG 
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA 
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG 
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT 
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG 
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG 
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC 
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC 
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT 
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA 
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG 
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG 
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC 
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG 
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC 
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC 
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC 
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG 
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC 
ACACTGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112486.1
CDS	23..1234
Translation	MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWATDALASVGITLG

Target ORF information:

RefSeq Version	NM_031209.3
Organism	Homo sapiens(human)
Definition	Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031209.3

ORF Insert Sequence:

ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG 
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA 
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG 
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT 
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG 
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG 
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC 
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC 
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT 
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA 
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG 
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG 
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC 
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG 
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC 
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC 
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC 
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG 
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC 
ACACTGGGCT GA