AJAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AJAP1
Entrez Gene ID	55966
Full Name	adherens junctions associated protein 1
Synonyms	MOT8,SHREW-1,SHREW1
General protein information	Preferred Names adherens junctions associated protein 1 Names adherens junction-associated protein 1 membrane protein shrew-1 transmembrane protein SHREW1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.32
Disorder MIM:	610972

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042478.1	NP_001035943.1	adherens junction-associated protein 1 precursor
NM_018836.4	NP_061324.1	adherens junction-associated protein 1 precursor
XM_011541786.2	XP_011540088.1	adherens junction-associated protein 1 isoform X1
NM_018836.3	NP_061324.1	adherens junction-associated protein 1 precursor
NM_001042478.2	NP_001035943.1	adherens junction-associated protein 1 precursor
XM_011541787.2	XP_011540089.1	adherens junction-associated protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AJAP1	XP_001153509.1
Canis lupus familiaris (dog)	AJAP1	XP_005620500.1
Homo sapiens (human)	AJAP1	NP_001035943.1
Bos taurus (cattle)	AJAP1	NP_001193744.1
Mus musculus (house mouse)	Ajap1	NP_001092769.1
Rattus norvegicus (Norway rat)	Ajap1	NP_001094484.1
Danio rerio (zebrafish)	LOC101887016	XP_005167007.1
Gallus gallus (chicken)	AJAP1	XP_004947415.1
Xenopus tropicalis (tropical clawed frog)	ajap1	NP_001072363.1

Gene Ontology
Bibliography

Related articles in PubMed

β-Catenin nuclear localization positively feeds back on EGF/EGFR-attenuated AJAP1 expression in breast cancer.
Xu C, Liu F, Xiang G, Cao L, Wang S, Liu J, Meng Q, Xu D, Lv S, Jiao J, Niu Y
Journal of experimental & clinical cancer research : CR38(1)238(2019 Jun)

AJAP1 expression modulates glioma cell motility and correlates with tumor growth and survival.
Di C, Mladkova N, Lin J, Fee B, Rivas M, Chunsheng K, Bigner D, Adamson DC
International journal of oncology52(1)47-54(2018 Jan)

EGFR/EGFRvIII remodels the cytoskeleton via epigenetic silencing of AJAP1 in glioma cells.
Yang C, Li YS, Wang QX, Huang K, Wei JW, Wang YF, Zhou JH, Yi KK, Zhang KL, Zhou BC, Liu C, Zeng L, Kang CS
Cancer letters403119-127(2017 09)

Deregulated AJAP1/β-catenin/ZEB1 signaling promotes hepatocellular carcinoma carcinogenesis and metastasis.
Han J, Xie C, Pei T, Wang J, Lan Y, Huang K, Cui Y, Wang F, Zhang J, Pan S, Liang Y, Zhen T, Song R, Sun B, Li Y, Shi H, Yang G, Liu X, Zhu M, Wang Y, Li K, Liu Y, Meng F, Liao F, Meng X, Hong X, Liu L
Cell death & disease8(4)e2736(2017 04)

Adherens junctions associated protein 1 serves as a predictor of recurrence of squamous cell carcinoma of the esophagus.
Tanaka H, Kanda M, Koike M, Iwata N, Shimizu D, Ezaka K, Sueoka S, Tanaka Y, Takami H, Hashimoto R, Tanaka C, Yamada S, Fujii T, Nakayama G, Sugimoto H, Fujiwara M, Kodera Y
International journal of oncology47(5)1811-8(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AJAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AJAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu69914	XM_011541787.2 Latest version!	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu25870	NM_001042478.2 Latest version!	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu25870	NM_018836.4 Latest version!	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu25870	XM_011541786.2 Latest version!	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu25870	NM_018836.3	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu25870	NM_001042478.1	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AJAP1 ( XM_011541787.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540089.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu69914
Clone ID Related Accession (Same CDS sequence)	XM_011541787.2
Accession Version	XM_011541787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC GCTGGCCGGG CCGCCCCCTC GGAAGCCATG CCTGGATACT GATAGCCATG 
TTTCAGCTCG CCGTGGACCT GCCCGCCTGT GAGGCCCTGG GCCCGGGGCC GGAGTTCTGG 
CTCCTGCCGC GGTCGCCGCC CCGGCCGCCC CGGCTGTGGA GTTTTAGGAG TGGACAGCCA 
GCGCGGGTCC CGGCCCCGGT GTGGAGCCCC CGGCCGCCCC GAGTGGAGCG GATCCACGGG 
CAGATGCAGA TGCCTCGAGC CAGACGGGCC CACAGGCCCC GGGACCAGGC GGCCGCCCTC 
GTGCCCAAGG CAGGACTGGC CAAGCCCCCA GCTGCTGCCA AATCCAGCCC TTCCCTCGCC 
TCTTCGTCCT CGTCCTCGTC CTCCGCGGTG GCCGGTGGGG CCCCGGAGCA GCAGGCCCTC 
CTGAGGAGGG GCAAGAGGCA CCTGCAGGGG GACGGTCTCA GCAGCTTCGA CTCCAGAGGC 
AGCCGGCCCA CCACAGAGAC TGAGTTCATC GCCTGGGGGC CCACGGGGGA CGAGGAGGCC 
CTGGAGTCCA ACACATTTCC GGGCGTTTAC GGCCCCACCA CGGTCTCCAT CCTACAAACA 
CGGAAGACAA CTGTGGCCGC CACCACCACC ACCACCACCA CGGCCACCCC CATGACGCTG 
CAGACTAAGG GGTTCACCGA GTCCTTGGAT CCCCGGAGAA GGATCCCAGG TGGGGTTAGC 
ACAACGGAGC CTTCCACCAG TCCCAGCAAC AACGGGGAAG TCACCCAGCC CCCAAGGATT 
CTGGGGGAGG CCTCAGGTCT GGCTGTCCAT CAGATCATCA CCATCACCGT CTCCCTCATC 
ATGGTCATAG CTGCTCTCAT CACAACTCTT GTCTTAAAAA ATTGCTGTGC CCAAAGCGGG 
AACACTCGTC GGAACAGCCA CCAGCGGAAG ACCAACCAGC AGGAGGAGAG CTGCCAGAAC 
CTCACGGACT TCCCCTCGGC CCGGGTGCCC AGCAGCCTGG ACATATTCAC GGCCTATAAC 
GAGACCCTGC AGTGTTCTCA CGAGTGCGTC AGGGCATCTG TGCCCGTGTA CACCGATGAG 
ACGCTGCACT CGACGACGGG GGAGTACAAA TCCACATTTA ATGGAAACCG ACCCTCCTCT 
TCTGATCGGC ATCTTATTCC TGTGGCCTTC GTGTCTGAGA AATGGTTTGA AATCTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540089.1
CDS	571..1773
Translation	MSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	XM_011541787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541787.2

ORF Insert Sequence:

ATGTCCATCC GCTGGCCGGG CCGCCCCCTC GGAAGCCATG CCTGGATACT GATAGCCATG 
TTTCAGCTCG CCGTGGACCT GCCCGCCTGT GAGGCCCTGG GCCCGGGGCC GGAGTTCTGG 
CTCCTGCCGC GGTCGCCGCC CCGGCCGCCC CGGCTGTGGA GTTTTAGGAG TGGACAGCCA 
GCGCGGGTCC CGGCCCCGGT GTGGAGCCCC CGGCCGCCCC GAGTGGAGCG GATCCACGGG 
CAGATGCAGA TGCCTCGAGC CAGACGGGCC CACAGGCCCC GGGACCAGGC GGCCGCCCTC 
GTGCCCAAGG CAGGACTGGC CAAGCCCCCA GCTGCTGCCA AATCCAGCCC TTCCCTCGCC 
TCTTCGTCCT CGTCCTCGTC CTCCGCGGTG GCCGGTGGGG CCCCGGAGCA GCAGGCCCTC 
CTGAGGAGGG GCAAGAGGCA CCTGCAGGGG GACGGTCTCA GCAGCTTCGA CTCCAGAGGC 
AGCCGGCCCA CCACAGAGAC TGAGTTCATC GCCTGGGGGC CCACGGGGGA CGAGGAGGCC 
CTGGAGTCCA ACACATTTCC GGGCGTTTAC GGCCCCACCA CGGTCTCCAT CCTACAAACA 
CGGAAGACAA CTGTGGCCGC CACCACCACC ACCACCACCA CGGCCACCCC CATGACGCTG 
CAGACTAAGG GGTTCACCGA GTCCTTGGAT CCCCGGAGAA GGATCCCAGG TGGGGTTAGC 
ACAACGGAGC CTTCCACCAG TCCCAGCAAC AACGGGGAAG TCACCCAGCC CCCAAGGATT 
CTGGGGGAGG CCTCAGGTCT GGCTGTCCAT CAGATCATCA CCATCACCGT CTCCCTCATC 
ATGGTCATAG CTGCTCTCAT CACAACTCTT GTCTTAAAAA ATTGCTGTGC CCAAAGCGGG 
AACACTCGTC GGAACAGCCA CCAGCGGAAG ACCAACCAGC AGGAGGAGAG CTGCCAGAAC 
CTCACGGACT TCCCCTCGGC CCGGGTGCCC AGCAGCCTGG ACATATTCAC GGCCTATAAC 
GAGACCCTGC AGTGTTCTCA CGAGTGCGTC AGGGCATCTG TGCCCGTGTA CACCGATGAG 
ACGCTGCACT CGACGACGGG GGAGTACAAA TCCACATTTA ATGGAAACCG ACCCTCCTCT 
TCTGATCGGC ATCTTATTCC TGTGGCCTTC GTGTCTGAGA AATGGTTTGA AATCTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AJAP1 ( NM_001042478.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035943.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25870
Clone ID Related Accession (Same CDS sequence)	NM_001042478.1 , NM_018836.4 , XM_011541786.2 , NM_018836.3 , NM_001042478.2
Accession Version	NM_001042478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529596800000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY282806.1, AF175409.1 and AL049673.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. CCDS Note: This CCDS ID represents the protein described in PMIDs: 14595118 and 17267690. This protein is encoded by two different splice variants that are supported by AF175409.1 and AL049673.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY282806.1, AF175409.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 14595118 ##RefSeq-Attributes-END##

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035943.1
CDS	382..1617
Translation	MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	NM_001042478.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042478.1

ORF Insert Sequence:

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AJAP1 ( NM_018836.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061324.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25870
Clone ID Related Accession (Same CDS sequence)	NM_001042478.1 , NM_018836.4 , XM_011541786.2 , NM_018836.3 , NM_001042478.2
Accession Version	NM_018836.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL391808.9, AY282806.1, AF175409.1 and AL023586.1. On May 17, 2019 this sequence version replaced NM_018836.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. CCDS Note: This CCDS ID represents the protein described in PMIDs: 14595118 and 17267690. This protein is encoded by two different splice variants that are supported by AF175409.1 and AL049673.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF175409.1, AY282806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378191.5/ ENSP00000367433.3 NMD candidate :: PMID: 14595118 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061324.1
CDS	818..2053
Translation	MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	NM_018836.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018836.4

ORF Insert Sequence:

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AJAP1 ( NM_018836.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061324.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25870
Clone ID Related Accession (Same CDS sequence)	NM_001042478.1 , NM_018836.4 , XM_011541786.2 , NM_018836.3 , NM_001042478.2
Accession Version	NM_018836.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529596800000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY282806.1, AF175409.1 and AL023586.1. On Jun 29, 2006 this sequence version replaced NM_018836.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. CCDS Note: This CCDS ID represents the protein described in PMIDs: 14595118 and 17267690. This protein is encoded by two different splice variants that are supported by AF175409.1 and AL049673.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY282806.1, AF175409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 14595118 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061324.1
CDS	382..1617
Translation	MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	NM_018836.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018836.3

ORF Insert Sequence:

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AJAP1 ( NM_001042478.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035943.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25870
Clone ID Related Accession (Same CDS sequence)	NM_001042478.1 , NM_018836.4 , XM_011541786.2 , NM_018836.3 , NM_001042478.2
Accession Version	NM_001042478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL391808.9, AY282806.1, AF175409.1, AL049673.1 and AL023586.1. On May 31, 2019 this sequence version replaced NM_001042478.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. CCDS Note: This CCDS ID represents the protein described in PMIDs: 14595118 and 17267690. This protein is encoded by two different splice variants that are supported by AF175409.1 and AL049673.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 14595118 ##RefSeq-Attributes-END##

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035943.1
CDS	818..2053
Translation	MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	NM_001042478.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042478.2

ORF Insert Sequence:

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AJAP1 ( XM_011541786.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540088.1 Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25870
Clone ID Related Accession (Same CDS sequence)	NM_001042478.1 , NM_018836.4 , XM_011541786.2 , NM_018836.3 , NM_001042478.2
Accession Version	XM_011541786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	adherens junction-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540088.1
CDS	652..1887
Translation	MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEALGPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAHRPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQGDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAATTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEASGLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTDFPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSSDRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	XM_011541786.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adherens junctions associated protein 1 (AJAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541786.2

ORF Insert Sequence:

ATGTGGATTC AACAGCTTTT AGGACTCAGC TCCATGTCCA TCCGCTGGCC GGGCCGCCCC 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGTTTCAGC TCGCCGTGGA CCTGCCCGCC 
TGTGAGGCCC TGGGCCCGGG GCCGGAGTTC TGGCTCCTGC CGCGGTCGCC GCCCCGGCCG 
CCCCGGCTGT GGAGTTTTAG GAGTGGACAG CCAGCGCGGG TCCCGGCCCC GGTGTGGAGC 
CCCCGGCCGC CCCGAGTGGA GCGGATCCAC GGGCAGATGC AGATGCCTCG AGCCAGACGG 
GCCCACAGGC CCCGGGACCA GGCGGCCGCC CTCGTGCCCA AGGCAGGACT GGCCAAGCCC 
CCAGCTGCTG CCAAATCCAG CCCTTCCCTC GCCTCTTCGT CCTCGTCCTC GTCCTCCGCG 
GTGGCCGGTG GGGCCCCGGA GCAGCAGGCC CTCCTGAGGA GGGGCAAGAG GCACCTGCAG 
GGGGACGGTC TCAGCAGCTT CGACTCCAGA GGCAGCCGGC CCACCACAGA GACTGAGTTC 
ATCGCCTGGG GGCCCACGGG GGACGAGGAG GCCCTGGAGT CCAACACATT TCCGGGCGTT 
TACGGCCCCA CCACGGTCTC CATCCTACAA ACACGGAAGA CAACTGTGGC CGCCACCACC 
ACCACCACCA CCACGGCCAC CCCCATGACG CTGCAGACTA AGGGGTTCAC CGAGTCCTTG 
GATCCCCGGA GAAGGATCCC AGGTGGGGTT AGCACAACGG AGCCTTCCAC CAGTCCCAGC 
AACAACGGGG AAGTCACCCA GCCCCCAAGG ATTCTGGGGG AGGCCTCAGG TCTGGCTGTC 
CATCAGATCA TCACCATCAC CGTCTCCCTC ATCATGGTCA TAGCTGCTCT CATCACAACT 
CTTGTCTTAA AAAATTGCTG TGCCCAAAGC GGGAACACTC GTCGGAACAG CCACCAGCGG 
AAGACCAACC AGCAGGAGGA GAGCTGCCAG AACCTCACGG ACTTCCCCTC GGCCCGGGTG 
CCCAGCAGCC TGGACATATT CACGGCCTAT AACGAGACCC TGCAGTGTTC TCACGAGTGC 
GTCAGGGCAT CTGTGCCCGT GTACACCGAT GAGACGCTGC ACTCGACGAC GGGGGAGTAC 
AAATCCACAT TTAATGGAAA CCGACCCTCC TCTTCTGATC GGCATCTTAT TCCTGTGGCC 
TTCGTGTCTG AGAAATGGTT TGAAATCTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

β-Catenin nuclear localization positively feeds back on EGF/EGFR-attenuated AJAP1 expression in breast cancer.
Journal of experimental & clinical cancer research : CR38(1)238(2019 Jun)
Xu C,Liu F,Xiang G,Cao L,Wang S,Liu J,Meng Q,Xu D,Lv S,Jiao J,Niu Y

AJAP1 expression modulates glioma cell motility and correlates with tumor growth and survival.
International journal of oncology52(1)47-54(2018 Jan)
Di C,Mladkova N,Lin J,Fee B,Rivas M,Chunsheng K,Bigner D,Adamson DC

EGFR/EGFRvIII remodels the cytoskeleton via epigenetic silencing of AJAP1 in glioma cells.
Cancer letters403119-127(2017 09)
Yang C,Li YS,Wang QX,Huang K,Wei JW,Wang YF,Zhou JH,Yi KK,Zhang KL,Zhou BC,Liu C,Zeng L,Kang CS

Deregulated AJAP1/β-catenin/ZEB1 signaling promotes hepatocellular carcinoma carcinogenesis and metastasis.
Cell death & disease8(4)e2736(2017 04)
Han J,Xie C,Pei T,Wang J,Lan Y,Huang K,Cui Y,Wang F,Zhang J,Pan S,Liang Y,Zhen T,Song R,Sun B,Li Y,Shi H,Yang G,Liu X,Zhu M,Wang Y,Li K,Liu Y,Meng F,Liao F,Meng X,Hong X,Liu L

Adherens junctions associated protein 1 serves as a predictor of recurrence of squamous cell carcinoma of the esophagus.
International journal of oncology47(5)1811-8(2015 Nov)
Tanaka H,Kanda M,Koike M,Iwata N,Shimizu D,Ezaka K,Sueoka S,Tanaka Y,Takami H,Hashimoto R,Tanaka C,Yamada S,Fujii T,Nakayama G,Sugimoto H,Fujiwara M,Kodera Y

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AJAP1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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