NSDHL cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSDHL
Entrez Gene ID	50814
Full Name	NAD(P) dependent steroid dehydrogenase-like
Synonyms	H105E3,SDR31E1,XAP104
General protein information	Preferred Names NAD(P) dependent steroid dehydrogenase-like Names sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating protein H105e3 short chain dehydrogenase/reductase family 31E, member 1
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: X Map Location: Xq28
Summary	The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	300275
Disorder Html:	CHILD syndrome, 308050 (3); CK syndrome, 300831 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017029564.1	XP_016885053.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X1
NM_015922.2	NP_057006.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
NM_001129765.1	NP_001123237.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
XM_011531178.2	XP_011529480.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X2
NM_015922.3	NP_057006.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
XM_011531178.1	XP_011529480.1	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C18513g	XP_453025.1
Homo sapiens (human)	NSDHL	NP_057006.1
Bos taurus (cattle)	NSDHL	NP_001030559.1
Canis lupus familiaris (dog)	NSDHL	XP_853073.2
Danio rerio (zebrafish)	nsdhl	NP_001017674.1
Xenopus tropicalis (tropical clawed frog)	nsdhl	NP_001017290.1
Magnaporthe oryzae (rice blast fungus)	MGG_11399	XP_003713491.1
Neurospora crassa	NCU02693	XP_965466.1
Schizosaccharomyces pombe (fission yeast)	SPBC3F6.02c	NP_596741.1
Eremothecium gossypii	AGOS_AFR001W	NP_985548.1
Pan troglodytes (chimpanzee)	NSDHL	XP_003317812.1
Gallus gallus (chicken)	NSDHL	XP_420279.1
Arabidopsis thaliana (thale cress)	3BETAHSD/D1	NP_849779.1
Oryza sativa (rice)	Os03g0405000	NP_001050327.1
Macaca mulatta (Rhesus monkey)	NSDHL	XP_001082183.1
Mus musculus (house mouse)	Nsdhl	NP_035071.3
Rattus norvegicus (Norway rat)	Nsdhl	NP_001009399.1
Saccharomyces cerevisiae (baker's yeast)	ERG26	NP_011514.1
Arabidopsis thaliana (thale cress)	3BETAHSD/D2	NP_180194.2

Gene Ontology
Bibliography

Related articles in PubMed

NSDHL-containing duplication at Xq28 in a male patient with autism spectrum disorder: a case report.
Hu CC, Sun YJ, Liu CX, Zhou BR, Li CY, Xu Q, Xu X
BMC medical genetics19(1)192(2018 10)

CHILD Syndrome: Case Report of a Chinese Patient and Literature Review of the NAD[P]H Steroid Dehydrogenase-Like Protein Gene Mutation.
Mi XB, Luo MX, Guo LL, Zhang TD, Qiu XW
Pediatric dermatology32(6)e277-82(2015 Nov-Dec)

Large deletions in the NSDHL gene in two patients with CHILD syndrome.
Yang Z, Hartmann B, Xu Z, Ma L, Happle R, Schlipf N, Zhang LX, Xu ZG, Wang ZY, Fischer J
Acta dermato-venereologica95(8)1007-8(2015 Nov)

A novel missense mutation in the NSDHL gene identified in a Lithuanian family by targeted next-generation sequencing causes CK syndrome.
Preiksaitiene E, Caro A, Benušienė E, Oltra S, Orellana C, Morkūnienė A, Roselló MP, Kasnauskiene J, Monfort S, Kučinskas V, Mayo S, Martinez F
American journal of medical genetics. Part A167(6)1342-8(2015 Jun)

Expression profile of NSDHL in human peripheral tissues.
Morimoto M, Souich Cd, Trinh J, McLarren KW, Boerkoel CF, Hendson G
Journal of molecular histology43(1)95-106(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NSDHL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSDHL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu99327	XM_017029564.1 Latest version!	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu16442	NM_015922.3 Latest version!	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu16442	NM_001129765.1 Latest version!	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu16442	XM_011531178.2 Latest version!	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu16442	NM_015922.2	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu16442	XM_011531178.1	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSDHL ( NM_015922.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_057006.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.

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CloneID	OHu16442
Clone ID Related Accession (Same CDS sequence)	NM_015922.2 , NM_001129765.1 , XM_011531178.2 , NM_015922.3 , XM_011531178.1
Accession Version	NM_015922.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U47105.2 and BC007816.2. On Jun 29, 2008 this sequence version replaced NM_015922.1. Transcript Variant: This variant (1) represents the more predominant transcript. Transcript variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U47105.2, SRR1660809.48665.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057006.1
CDS	195..1316
Translation	MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	NM_015922.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015922.2

ORF Insert Sequence:

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSDHL ( XM_011531178.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011529480.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu16442
Clone ID Related Accession (Same CDS sequence)	NM_015922.2 , NM_001129765.1 , XM_011531178.2 , NM_015922.3 , XM_011531178.1
Accession Version	XM_011531178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426003200000
Organism	Homo sapiens(Human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X1
Comment

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529480.1
Translation	MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	XM_011531178.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531178.1

ORF Insert Sequence:

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSDHL ( XM_017029564.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016885053.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu99327
Clone ID Related Accession (Same CDS sequence)	XM_017029564.1
Accession Version	XM_017029564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG AAAAGTTGAT TACAAACGGG ACCATATTTT GCTTCGAAAT GGAACCAGCA 
GTTAGCGAGC CAATGAGAGA CCAAGTCGCA CGGACTCATT TGACAGAGGA CACTCCCAAA 
GTGAATGCTG ACATAGAAAA GGTTAACCAG AATCAGGCCA AGAGATGCAC AGTGATCGGT 
GGCTCTGGAT TCCTGGGGCA GCACATGGTG GAGCAGTTGC TGGCAAGAGG ATATGCTGTC 
AATGTATTTG ATATCCAGCA AGGGTTTGAT AATCCCCAGG TGCGGTTCTT TCTGGGTGAC 
CTCTGCAGCC GACAGGATCT GTACCCAGCT CTGAAAGGTG TAAACACAGT TTTCCACTGT 
GCGTCACCCC CACCATCCAG TAACAACAAG GAGCTCTTTT ATAGAGTGAA TTACATTGGC 
ACCAAGAATG TCATTGAAAC TTGCAAAGAG GCTGGGGTTC AGAAACTCAT TTTAACCAGC 
AGTGCCAGTG TCATCTTTGA GGGCGTCGAT ATCAAGAATG GAACTGAAGA CCTTCCCTAT 
GCCATGAAAC CCATTGACTA CTACACAGAG ACTAAGATCT TACAGGAGAG GGCAGTTCTG 
GGCGCCAACG ATCCTGAGAA GAATTTCTTA ACCACAGCCA TCCGCCCTCA TGGCATTTTC 
GGCCCAAGGG ACCCGCAGTT GGTACCCATC CTCATCGAGG CAGCCAGGAA CGGCAAGATG 
AAGTTCGTGA TTGGAAATGG GAAGAACTTG GTGGACTTCA CCTTTGTGGA GAACGTGGTC 
CATGGACACA TCCTGGCGGC AGAGCAGCTC TCCCGAGACT CGACACTGGG TGGGAAGGCA 
TTTCACATCA CCAATGATGA GCCCATCCCT TTCTGGACAT TCCTGTCTCG CATCCTGACA 
GGCCTCAATT ATGAGGCCCC CAAGTACCAC ATCCCCTACT GGGTGGCCTA CTACCTGGCC 
CTCCTGCTAT CCCTGCTGGT GATGGTGATC AGTCCTGTCA TCCAGCTGCA GCCCACCTTC 
ACACCCATGC GGGTCGCACT GGCTGGCACA TTCCACTACT ACAGCTGCGA GAGAGCCAAA 
AAGGCCATGG GCTACCAGCC ACTAGTGACC ATGGATGATG CTATGGAGAG GACCGTGCAG 
AGCTTTCGCC ACCTGCGGAG GGTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885053.1
CDS	79..1248
Translation	MRKEKLITNGTIFCFEMEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	XM_017029564.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029564.1

ORF Insert Sequence:

ATGAGAAAAG AAAAGTTGAT TACAAACGGG ACCATATTTT GCTTCGAAAT GGAACCAGCA 
GTTAGCGAGC CAATGAGAGA CCAAGTCGCA CGGACTCATT TGACAGAGGA CACTCCCAAA 
GTGAATGCTG ACATAGAAAA GGTTAACCAG AATCAGGCCA AGAGATGCAC AGTGATCGGT 
GGCTCTGGAT TCCTGGGGCA GCACATGGTG GAGCAGTTGC TGGCAAGAGG ATATGCTGTC 
AATGTATTTG ATATCCAGCA AGGGTTTGAT AATCCCCAGG TGCGGTTCTT TCTGGGTGAC 
CTCTGCAGCC GACAGGATCT GTACCCAGCT CTGAAAGGTG TAAACACAGT TTTCCACTGT 
GCGTCACCCC CACCATCCAG TAACAACAAG GAGCTCTTTT ATAGAGTGAA TTACATTGGC 
ACCAAGAATG TCATTGAAAC TTGCAAAGAG GCTGGGGTTC AGAAACTCAT TTTAACCAGC 
AGTGCCAGTG TCATCTTTGA GGGCGTCGAT ATCAAGAATG GAACTGAAGA CCTTCCCTAT 
GCCATGAAAC CCATTGACTA CTACACAGAG ACTAAGATCT TACAGGAGAG GGCAGTTCTG 
GGCGCCAACG ATCCTGAGAA GAATTTCTTA ACCACAGCCA TCCGCCCTCA TGGCATTTTC 
GGCCCAAGGG ACCCGCAGTT GGTACCCATC CTCATCGAGG CAGCCAGGAA CGGCAAGATG 
AAGTTCGTGA TTGGAAATGG GAAGAACTTG GTGGACTTCA CCTTTGTGGA GAACGTGGTC 
CATGGACACA TCCTGGCGGC AGAGCAGCTC TCCCGAGACT CGACACTGGG TGGGAAGGCA 
TTTCACATCA CCAATGATGA GCCCATCCCT TTCTGGACAT TCCTGTCTCG CATCCTGACA 
GGCCTCAATT ATGAGGCCCC CAAGTACCAC ATCCCCTACT GGGTGGCCTA CTACCTGGCC 
CTCCTGCTAT CCCTGCTGGT GATGGTGATC AGTCCTGTCA TCCAGCTGCA GCCCACCTTC 
ACACCCATGC GGGTCGCACT GGCTGGCACA TTCCACTACT ACAGCTGCGA GAGAGCCAAA 
AAGGCCATGG GCTACCAGCC ACTAGTGACC ATGGATGATG CTATGGAGAG GACCGTGCAG 
AGCTTTCGCC ACCTGCGGAG GGTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSDHL ( NM_001129765.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001123237.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 2, mRNA.

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CloneID	OHu16442
Clone ID Related Accession (Same CDS sequence)	NM_015922.2 , NM_001129765.1 , XM_011531178.2 , NM_015922.3 , XM_011531178.1
Accession Version	NM_001129765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Homo sapiens(human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U47105.2, U82671.5 and BC007816.2. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an additional 5' non-coding exon, hence has a longer 5' UTR compared to variant 1. Transcript variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.240566.1, SRR1163655.165455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123237.1
CDS	262..1383
Translation	MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	NM_001129765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129765.1

ORF Insert Sequence:

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSDHL ( NM_015922.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_057006.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.

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CloneID	OHu16442
Clone ID Related Accession (Same CDS sequence)	NM_015922.2 , NM_001129765.1 , XM_011531178.2 , NM_015922.3 , XM_011531178.1
Accession Version	NM_015922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Homo sapiens(human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U47105.2, BC007816.2 and U82671.5. On Nov 22, 2018 this sequence version replaced NM_015922.2. Transcript Variant: This variant (1) represents the more predominant transcript. Transcript variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026549.1, SRR1803615.156504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370274.8/ ENSP00000359297.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057006.1
CDS	99..1220
Translation	MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	NM_015922.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015922.3

ORF Insert Sequence:

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSDHL ( XM_011531178.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011529480.1 Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X2, mRNA.

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CloneID	OHu16442
Clone ID Related Accession (Same CDS sequence)	NM_015922.2 , NM_001129765.1 , XM_011531178.2 , NM_015922.3 , XM_011531178.1
Accession Version	XM_011531178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529480.1
CDS	322..1443
Translation	MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Target ORF information:

RefSeq Version	XM_011531178.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531178.2

ORF Insert Sequence:

ATGGAACCAG CAGTTAGCGA GCCAATGAGA GACCAAGTCG CACGGACTCA TTTGACAGAG 
GACACTCCCA AAGTGAATGC TGACATAGAA AAGGTTAACC AGAATCAGGC CAAGAGATGC 
ACAGTGATCG GTGGCTCTGG ATTCCTGGGG CAGCACATGG TGGAGCAGTT GCTGGCAAGA 
GGATATGCTG TCAATGTATT TGATATCCAG CAAGGGTTTG ATAATCCCCA GGTGCGGTTC 
TTTCTGGGTG ACCTCTGCAG CCGACAGGAT CTGTACCCAG CTCTGAAAGG TGTAAACACA 
GTTTTCCACT GTGCGTCACC CCCACCATCC AGTAACAACA AGGAGCTCTT TTATAGAGTG 
AATTACATTG GCACCAAGAA TGTCATTGAA ACTTGCAAAG AGGCTGGGGT TCAGAAACTC 
ATTTTAACCA GCAGTGCCAG TGTCATCTTT GAGGGCGTCG ATATCAAGAA TGGAACTGAA 
GACCTTCCCT ATGCCATGAA ACCCATTGAC TACTACACAG AGACTAAGAT CTTACAGGAG 
AGGGCAGTTC TGGGCGCCAA CGATCCTGAG AAGAATTTCT TAACCACAGC CATCCGCCCT 
CATGGCATTT TCGGCCCAAG GGACCCGCAG TTGGTACCCA TCCTCATCGA GGCAGCCAGG 
AACGGCAAGA TGAAGTTCGT GATTGGAAAT GGGAAGAACT TGGTGGACTT CACCTTTGTG 
GAGAACGTGG TCCATGGACA CATCCTGGCG GCAGAGCAGC TCTCCCGAGA CTCGACACTG 
GGTGGGAAGG CATTTCACAT CACCAATGAT GAGCCCATCC CTTTCTGGAC ATTCCTGTCT 
CGCATCCTGA CAGGCCTCAA TTATGAGGCC CCCAAGTACC ACATCCCCTA CTGGGTGGCC 
TACTACCTGG CCCTCCTGCT ATCCCTGCTG GTGATGGTGA TCAGTCCTGT CATCCAGCTG 
CAGCCCACCT TCACACCCAT GCGGGTCGCA CTGGCTGGCA CATTCCACTA CTACAGCTGC 
GAGAGAGCCA AAAAGGCCAT GGGCTACCAG CCACTAGTGA CCATGGATGA TGCTATGGAG 
AGGACCGTGC AGAGCTTTCG CCACCTGCGG AGGGTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NSDHL-containing duplication at Xq28 in a male patient with autism spectrum disorder: a case report.
BMC medical genetics19(1)192(2018 10)
Hu CC,Sun YJ,Liu CX,Zhou BR,Li CY,Xu Q,Xu X

CHILD Syndrome: Case Report of a Chinese Patient and Literature Review of the NAD[P]H Steroid Dehydrogenase-Like Protein Gene Mutation.
Pediatric dermatology32(6)e277-82(2015 Nov-Dec)
Mi XB,Luo MX,Guo LL,Zhang TD,Qiu XW

Large deletions in the NSDHL gene in two patients with CHILD syndrome.
Acta dermato-venereologica95(8)1007-8(2015 Nov)
Yang Z,Hartmann B,Xu Z,Ma L,Happle R,Schlipf N,Zhang LX,Xu ZG,Wang ZY,Fischer J

A novel missense mutation in the NSDHL gene identified in a Lithuanian family by targeted next-generation sequencing causes CK syndrome.
American journal of medical genetics. Part A167(6)1342-8(2015 Jun)
Preiksaitiene E,Caro A,Benušienė E,Oltra S,Orellana C,Morkūnienė A,Roselló MP,Kasnauskiene J,Monfort S,Kučinskas V,Mayo S,Martinez F

Expression profile of NSDHL in human peripheral tissues.
Journal of molecular histology43(1)95-106(2012 Feb)
Morimoto M,Souich Cd,Trinh J,McLarren KW,Boerkoel CF,Hendson G

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NSDHL cDNA ORF clone, Homo sapiens(Human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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