HSD3B2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSD3B2
Entrez Gene ID	3284
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Synonyms	HSD3B,HSDB,SDR11E2
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 3 beta-HSD type II 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II 3-beta-HSD II 3-beta-HSD adrenal and gonadal type 3-beta-hydroxy-5-ene steroid dehydrogenase 3-beta-hydroxy-Delta(5)-steroid dehydrogenase delta 5-delta 4-isomerase type II progesterone reductase short chain dehydrogenase/reductase family 11E, member 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p12
Summary	The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009].
Disorder MIM:	613890
Disorder Html:	Adrenal hyperplasia, congenital, due to 3-beta-hydroxysteroid dehydrogenase 2 deficiency, 201810 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000198.3	NP_000189.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
NM_001166120.1	NP_001159592.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
NM_000198.4	NP_000189.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hsd3b	NP_001036084.1
Danio rerio (zebrafish)	si:rp71-68n21.10	XP_694204.3
Danio rerio (zebrafish)	hsd3b1	NP_997962.1
Xenopus tropicalis (tropical clawed frog)	LOC100490622	XP_004920800.1
Gallus gallus (chicken)	HSD3B2	NP_990449.1
Rattus norvegicus (Norway rat)	LOC100911154	XP_006233083.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006654	XP_316685.4
Mus musculus (house mouse)	Hsd3b1	NP_032319.1
Rattus norvegicus (Norway rat)	Hsd3b6	NP_058961.4
Macaca mulatta (Rhesus monkey)	LOC100424395	XP_002801771.1
Homo sapiens (human)	HSD3B2	NP_001159592.1
Pan troglodytes (chimpanzee)	HSD3B2	XP_001142555.1
Canis lupus familiaris (dog)	HSD3B2	NP_001010954.1
Bos taurus (cattle)	HSD3B1	NP_776768.1
Drosophila melanogaster (fruit fly)	CG7724	NP_648957.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of Cryptochrome-1 and Cryptochrome-2 in Aldosterone-Producing Adenomas and Adrenocortical Cells.
Tetti M, Castellano I, Venziano F, Magnino C, Veglio F, Mulatero P, Monticone S
International journal of molecular sciences19(6)(2018 Jun)

HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR expression in infertile women with endometriosis.
Osiński M, Wirstlein P, Wender-Ożegowska E, Mikołajczyk M, Jagodziński PP, Szczepańska M
Ginekologia polska89(3)125-134(2018)

Testicular Adrenal Rest Tumor in Two Brothers with a Novel Mutation in the 3-Beta-Hydroxysteroid Dehydrogenase-2 Gene.
Güven A, Polat S
Journal of clinical research in pediatric endocrinology9(1)85-90(2017 Mar)

DNA methylation is not involved in specific down-regulation of HSD3B2, NR4A1 and RARB genes in androgen-secreting cells of human adrenal cortex.
Baquedano MS, Perez Garrido N, Goñi J, Saraco N, Aliberti P, Berensztein E, Rivarola MA, Belgorosky A
Molecular and cellular endocrinology44146-54(2017 02)

A New Homozygous Frameshift Mutation in the HSD3B2 Gene in an Apparently Nonconsanguineous Italian Family.
Bizzarri C, Massimi A, Federici L, Cualbu A, Loche S, Bellincampi L, Bernardini S, Cappa M, Porzio O
Hormone research in paediatrics86(1)53-61(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HSD3B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSD3B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14034	NM_001166120.1 Latest version!	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14034	NM_000198.4 Latest version!	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu14034	NM_000198.3	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSD3B2 ( NM_000198.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000189.1 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14034
Clone ID Related Accession (Same CDS sequence)	NM_000198.3 , NM_001166120.1 , NM_000198.4
Accession Version	NM_000198.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512403200000
Organism	Homo sapiens(human)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV705363.1, BC038419.1 and AK222997.1. On Oct 8, 2009 this sequence version replaced NM_000198.2. Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222997.1, BC038419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000189.1
CDS	173..1291
Translation	MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ

Target ORF information:

RefSeq Version	NM_000198.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000198.3

ORF Insert Sequence:

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSD3B2 ( NM_001166120.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159592.1 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14034
Clone ID Related Accession (Same CDS sequence)	NM_000198.3 , NM_001166120.1 , NM_000198.4
Accession Version	NM_001166120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP197992.1, BC038419.1 and AK222997.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an alternate 5' non-coding exon, hence differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA015880.1, DA432316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159592.1
CDS	250..1368
Translation	MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ

Target ORF information:

RefSeq Version	NM_001166120.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166120.1

ORF Insert Sequence:

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSD3B2 ( NM_000198.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000189.1 Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14034
Clone ID Related Accession (Same CDS sequence)	NM_000198.3 , NM_001166120.1 , NM_000198.4
Accession Version	NM_000198.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV705363.1, BC038419.1 and AK222997.1. On Nov 23, 2018 this sequence version replaced NM_000198.3. Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313413.1, AK222997.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369416.4/ ENSP00000358424.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000189.1
CDS	143..1261
Translation	MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ

Target ORF information:

RefSeq Version	NM_000198.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000198.4

ORF Insert Sequence:

ATGGGCTGGA GCTGCCTTGT GACAGGAGCA GGAGGGCTTC TGGGTCAGAG GATCGTCCGC 
CTGTTGGTGG AAGAGAAGGA ACTGAAGGAG ATCAGGGCCT TGGACAAGGC CTTCAGACCA 
GAATTGAGAG AGGAATTTTC TAAGCTCCAG AACAGGACCA AGCTGACTGT ACTTGAAGGA 
GACATTCTGG ATGAGCCATT CCTGAAAAGA GCCTGCCAGG ACGTCTCGGT CGTCATCCAC 
ACCGCCTGTA TCATTGATGT CTTTGGTGTC ACTCACAGAG AGTCCATCAT GAATGTCAAT 
GTGAAAGGTA CCCAGCTACT GTTGGAGGCC TGTGTCCAAG CCAGTGTGCC AGTCTTCATC 
TACACCAGTA GCATAGAGGT AGCCGGGCCC AACTCCTACA AGGAAATCAT CCAGAACGGC 
CACGAAGAAG AGCCTCTGGA AAACACATGG CCCACTCCAT ACCCGTACAG CAAAAAGCTT 
GCTGAGAAGG CTGTGCTGGC GGCTAATGGG TGGAATCTAA AAAATGGTGA TACCTTGTAC 
ACTTGTGCGT TAAGACCCAC ATATATCTAT GGGGAAGGAG GCCCATTCCT TTCTGCCAGT 
ATAAATGAGG CCCTGAACAA CAATGGGATC CTGTCAAGTG TTGGAAAGTT CTCTACAGTC 
AACCCAGTCT ATGTTGGCAA CGTGGCCTGG GCCCACATTC TGGCCTTGAG GGCTCTGCGG 
GACCCCAAGA AGGCCCCAAG TGTCCGAGGT CAATTCTATT ACATCTCAGA TGACACGCCT 
CACCAAAGCT ATGATAACCT TAATTACATC CTGAGCAAAG AGTTTGGCCT CCGCCTTGAT 
TCCAGATGGA GCCTTCCTTT AACCCTGATG TACTGGATTG GCTTCCTGCT GGAAGTAGTG 
AGCTTCCTAC TCAGCCCAAT TTACTCCTAT CAACCCCCCT TCAACCGCCA CACAGTCACA 
TTATCAAATA GTGTGTTCAC CTTCTCTTAC AAGAAGGCTC AGCGAGATCT GGCGTATAAG 
CCACTCTACA GCTGGGAGGA AGCCAAGCAG AAAACCGTGG AGTGGGTTGG TTCCCTTGTG 
GACCGGCACA AGGAGACCCT GAAGTCCAAG ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of Cryptochrome-1 and Cryptochrome-2 in Aldosterone-Producing Adenomas and Adrenocortical Cells.
International journal of molecular sciences19(6)(2018 Jun)
Tetti M,Castellano I,Venziano F,Magnino C,Veglio F,Mulatero P,Monticone S

HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR expression in infertile women with endometriosis.
Ginekologia polska89(3)125-134(2018)
Osiński M,Wirstlein P,Wender-Ożegowska E,Mikołajczyk M,Jagodziński PP,Szczepańska M

Testicular Adrenal Rest Tumor in Two Brothers with a Novel Mutation in the 3-Beta-Hydroxysteroid Dehydrogenase-2 Gene.
Journal of clinical research in pediatric endocrinology9(1)85-90(2017 Mar)
Güven A,Polat S

DNA methylation is not involved in specific down-regulation of HSD3B2, NR4A1 and RARB genes in androgen-secreting cells of human adrenal cortex.
Molecular and cellular endocrinology44146-54(2017 02)
Baquedano MS,Perez Garrido N,Goñi J,Saraco N,Aliberti P,Berensztein E,Rivarola MA,Belgorosky A

A New Homozygous Frameshift Mutation in the HSD3B2 Gene in an Apparently Nonconsanguineous Italian Family.
Hormone research in paediatrics86(1)53-61(2016)
Bizzarri C,Massimi A,Federici L,Cualbu A,Loche S,Bellincampi L,Bernardini S,Cappa M,Porzio O

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HSD3B2 cDNA ORF clone, Homo sapiens(human)

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