Hsd3b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b1
Entrez Gene ID	15492
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Synonyms	3-beta-HSD I,D3Ertd383e
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 F2.2\|3 42.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501036.2	XP_006501099.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 isoform X1
XM_006501036.3	XP_006501099.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 isoform X1
NM_001304800.1	NP_001291729.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NM_008293.4	NP_032319.1	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hsd3b	NP_001036084.1
Danio rerio (zebrafish)	si:rp71-68n21.10	XP_694204.3
Danio rerio (zebrafish)	hsd3b1	NP_997962.1
Xenopus tropicalis (tropical clawed frog)	LOC100490622	XP_004920800.1
Gallus gallus (chicken)	HSD3B2	NP_990449.1
Rattus norvegicus (Norway rat)	LOC100911154	XP_006233083.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006654	XP_316685.4
Mus musculus (house mouse)	Hsd3b1	NP_032319.1
Rattus norvegicus (Norway rat)	Hsd3b6	NP_058961.4
Macaca mulatta (Rhesus monkey)	LOC100424395	XP_002801771.1
Homo sapiens (human)	HSD3B2	NP_001159592.1
Pan troglodytes (chimpanzee)	HSD3B2	XP_001142555.1
Canis lupus familiaris (dog)	HSD3B2	NP_001010954.1
Bos taurus (cattle)	HSD3B1	NP_776768.1
Drosophila melanogaster (fruit fly)	CG7724	NP_648957.1

Gene Ontology
Bibliography

Related articles in PubMed

Steroidogenic differentiation and PKA signaling are programmed by histone methyltransferase EZH2 in the adrenal cortex.
Mathieu M, Drelon C, Rodriguez S, Tabbal H, Septier A, Damon-Soubeyrand C, Dumontet T, Berthon A, Sahut-Barnola I, Djari C, Batisse-Lignier M, Pointud JC, Richard D, Kerdivel G, Calméjane MA, Boeva V, Tauveron I, Lefrançois-Martinez AM, Martinez A, Val P
Proceedings of the National Academy of Sciences of the United States of America115(52)E12265-E12274(2018 12)

Fetal Leydig cells dedifferentiate and serve as adult Leydig stem cells.
Shima Y, Miyabayashi K, Sato T, Suyama M, Ohkawa Y, Doi M, Okamura H, Suzuki K
Development (Cambridge, England)145(23)(2018 12)

A perivascular niche for multipotent progenitors in the fetal testis.
Kumar DL, DeFalco T
Nature communications9(1)4519(2018 10)

Forebrain Ptf1a Is Required for Sexual Differentiation of the Brain.
Fujiyama T, Miyashita S, Tsuneoka Y, Kanemaru K, Kakizaki M, Kanno S, Ishikawa Y, Yamashita M, Owa T, Nagaoka M, Kawaguchi Y, Yanagawa Y, Magnuson MA, Muratani M, Shibuya A, Nabeshima YI, Yanagisawa M, Funato H, Hoshino M
Cell reports24(1)79-94(2018 07)

MRAP deficiency impairs adrenal progenitor cell differentiation and gland zonation.
Novoselova TV, Hussain M, King PJ, Guasti L, Metherell LA, Charalambous M, Clark AJL, Chan LF
FASEB journal : official publication of the Federation of American Societies for Experimental Biologyfj201701274RR(2018 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsd3b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01673	NM_001304800.1 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu01673	NM_008293.4 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu01673	XM_006501036.3 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu01673	XM_006501036.2	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b1 ( NM_001304800.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291729.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01673
Clone ID Related Accession (Same CDS sequence)	XM_006501036.2 , XM_006501036.3 , NM_001304800.1 , NM_008293.4
Accession Version	NM_001304800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK146665.1, AL671965.6 and BC052659.1. On Feb 15, 2015 this sequence version replaced XM_006501035.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146665.1, CF551331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291729.1
CDS	186..1307
Translation	MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDVAGPNSYKKIVLNGHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKGILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRPNASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHLITLSNSTFTFSYKKAQRDLGYEPLVNWEEAKQKTSEWIGTIVEQHREILDTKCQ

Target ORF information:

RefSeq Version	NM_001304800.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304800.1

ORF Insert Sequence:

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b1 ( NM_008293.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032319.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01673
Clone ID Related Accession (Same CDS sequence)	XM_006501036.2 , XM_006501036.3 , NM_001304800.1 , NM_008293.4
Accession Version	NM_008293.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147114.1 and BC052659.1. On Feb 12, 2015 this sequence version replaced NM_008293.3. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147114.1, BM453585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032319.1
CDS	376..1497
Translation	MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDVAGPNSYKKIVLNGHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKGILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRPNASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHLITLSNSTFTFSYKKAQRDLGYEPLVNWEEAKQKTSEWIGTIVEQHREILDTKCQ

Target ORF information:

RefSeq Version	NM_008293.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008293.4

ORF Insert Sequence:

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b1 ( XM_006501036.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501099.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01673
Clone ID Related Accession (Same CDS sequence)	XM_006501036.2 , XM_006501036.3 , NM_001304800.1 , NM_008293.4
Accession Version	XM_006501036.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501099.1
CDS	371..1492
Translation	MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDVAGPNSYKKIVLNGHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKGILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRPNASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHLITLSNSTFTFSYKKAQRDLGYEPLVNWEEAKQKTSEWIGTIVEQHREILDTKCQ

Target ORF information:

RefSeq Version	XM_006501036.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501036.2

ORF Insert Sequence:

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b1 ( XM_006501036.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501099.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.

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CloneID	OMu01673
Clone ID Related Accession (Same CDS sequence)	XM_006501036.2 , XM_006501036.3 , NM_001304800.1 , NM_008293.4
Accession Version	XM_006501036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501099.1
CDS	371..1492
Translation	MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDVAGPNSYKKIVLNGHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKGILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRPNASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHLITLSNSTFTFSYKKAQRDLGYEPLVNWEEAKQKTSEWIGTIVEQHREILDTKCQ

Target ORF information:

RefSeq Version	XM_006501036.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501036.3

ORF Insert Sequence:

ATGGCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
AAGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGAG CTCTGGACAA AGTATTCCGA 
CCAGAAACCA AGGAGGAATT CTCCAAGCTG CAGACAAAGA CCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TGGATGCCCA GTGCCTGAGG AGAGCCTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCTG CTGTCATTGA TGTCACAGGT GTCATTCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CCTATTGGAG GCCTGTGTTC AAGCAAGTGT GCCAGCCTTC 
ATCTTCTGCA GCTCAGTTGA TGTTGCAGGG CCCAACTCGT ACAAGAAGAT CGTCCTGAAT 
GGCCACGAGG AACAGAATCA TGAAAGCACA TGGTCTGATC CATACCCATA CAGCAAAAAG 
ATGGCTGAGA AGGCAGTACT GGCAGCCAAT GGGAGCATGC TGAAAAATGG TGGCACTTTG 
AATACTTGTG CCTTAAGACC CATGTACATT TATGGGGAGA GAAGTCCATT CATTTTTAAT 
GCAATAATTA GGGCCCTCAA AAATAAGGGT ATTCTGTGTG TTACTGGCAA ATTCTCCATA 
GCCAACCCAG TATATGTGGA AAATGTGGCC TGGGCACACA TTCTGGCAGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCTAC AAGCATCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAC ACCCTGAGTA AGGAATGGGG GCTCCGCCCT 
AATGCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTACGTCC AGTCTACAGG TATAGACCTC TCTTTAACCG CCACTTGATC 
ACACTGTCAA ATAGCACGTT CACTTTTTCT TACAAGAAAG CTCAGCGAGA TCTGGGCTAT 
GAGCCACTTG TCAACTGGGA GGAAGCAAAG CAGAAAACCT CAGAGTGGAT AGGGACAATA 
GTGGAACAGC ACAGGGAGAT ATTGGACACA AAGTGCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Steroidogenic differentiation and PKA signaling are programmed by histone methyltransferase EZH2 in the adrenal cortex.
Proceedings of the National Academy of Sciences of the United States of America115(52)E12265-E12274(2018 12)
Mathieu M,Drelon C,Rodriguez S,Tabbal H,Septier A,Damon-Soubeyrand C,Dumontet T,Berthon A,Sahut-Barnola I,Djari C,Batisse-Lignier M,Pointud JC,Richard D,Kerdivel G,Calméjane MA,Boeva V,Tauveron I,Lefrançois-Martinez AM,Martinez A,Val P

Fetal Leydig cells dedifferentiate and serve as adult Leydig stem cells.
Development (Cambridge, England)145(23)(2018 12)
Shima Y,Miyabayashi K,Sato T,Suyama M,Ohkawa Y,Doi M,Okamura H,Suzuki K

A perivascular niche for multipotent progenitors in the fetal testis.
Nature communications9(1)4519(2018 10)
Kumar DL,DeFalco T

Forebrain Ptf1a Is Required for Sexual Differentiation of the Brain.
Cell reports24(1)79-94(2018 07)
Fujiyama T,Miyashita S,Tsuneoka Y,Kanemaru K,Kakizaki M,Kanno S,Ishikawa Y,Yamashita M,Owa T,Nagaoka M,Kawaguchi Y,Yanagawa Y,Magnuson MA,Muratani M,Shibuya A,Nabeshima YI,Yanagisawa M,Funato H,Hoshino M

MRAP deficiency impairs adrenal progenitor cell differentiation and gland zonation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biologyfj201701274RR(2018 Jun)
Novoselova TV,Hussain M,King PJ,Guasti L,Metherell LA,Charalambous M,Clark AJL,Chan LF

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Hsd3b1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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