HCCS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCCS
Entrez Gene ID	3052
Full Name	holocytochrome c synthase
Synonyms	CCHL,LSDMCA1,MCOPS7,MLS
General protein information	Preferred Names holocytochrome c synthase Names cytochrome c-type heme lyase cytochrome c heme-lyase holocytochrome c-type synthase microphthalamia with linear skin defects
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.2
Summary	The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010].
Disorder MIM:	300056
Disorder Html:	Linear skin defects with multiple congenital anomalies 1, 309801 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024452368.1	XP_024308136.1	cytochrome c-type heme lyase isoform X1
NM_001171991.2	NP_001165462.1	cytochrome c-type heme lyase
NM_005333.5	NP_005324.3	cytochrome c-type heme lyase
NM_001122608.2	NP_001116080.1	cytochrome c-type heme lyase
NM_001122608.3	NP_001116080.1	cytochrome c-type heme lyase
NM_001171991.3	NP_001165462.1	cytochrome c-type heme lyase
NM_005333.4	NP_005324.3	cytochrome c-type heme lyase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HCCS	NP_001092356.1
Macaca mulatta (Rhesus monkey)	HCCS	XP_001094078.1
Mus musculus (house mouse)	Hccs	NP_032248.3
Kluyveromyces lactis	KLLA0F25894g	XP_456233.1
Canis lupus familiaris (dog)	HCCS	XP_005641157.1
Pan troglodytes (chimpanzee)	LOC465489	XP_520928.3
Xenopus tropicalis (tropical clawed frog)	hccs	NP_001234907.1
Neurospora crassa	NCU08138	XP_959445.2
Homo sapiens (human)	HCCS	NP_001116080.1
Rattus norvegicus (Norway rat)	Hccs	NP_001178661.1
Gallus gallus (chicken)	HCCS	NP_001026446.1
Danio rerio (zebrafish)	hccs	NP_958859.1
Saccharomyces cerevisiae (baker's yeast)	CYT2	NP_012836.1
Eremothecium gossypii	AGOS_ADR154W	NP_984251.2
Schizosaccharomyces pombe (fission yeast)	SPAC24C9.02c	NP_594026.1

Gene Ontology
Bibliography

Related articles in PubMed

Biosynthesis of Single Thioether c-Type Cytochromes Provides Insight into Mechanisms Intrinsic to Holocytochrome c Synthase (HCCS).
Babbitt SE, Hsu J, Mendez DL, Kranz RG
Biochemistry56(26)3337-3346(2017 07)

Molecular Basis Behind Inability of Mitochondrial Holocytochrome c Synthase to Mature Bacterial Cytochromes: DEFINING A CRITICAL ROLE FOR CYTOCHROME c α HELIX-1.
Babbitt SE, Hsu J, Kranz RG
The Journal of biological chemistry291(34)17523-34(2016 08)

Mechanisms of mitochondrial holocytochrome c synthase and the key roles played by cysteines and histidine of the heme attachment site, Cys-XX-Cys-His.
Babbitt SE, San Francisco B, Mendez DL, Lukat-Rodgers GS, Rodgers KR, Bretsnyder EC, Kranz RG
The Journal of biological chemistry289(42)28795-807(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HCCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu106260	XM_024452368.1 Latest version!	Homo sapiens holocytochrome c synthase (HCCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu27077	NM_005333.5 Latest version!	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu27077	NM_001122608.3 Latest version!	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu27077	NM_001171991.3 Latest version!	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
View Old Accession Versions >>
OHu27077	NM_001122608.2	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu27077	NM_001171991.2	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu27077	NM_005333.4	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCCS ( XM_024452368.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308136.1 Homo sapiens holocytochrome c synthase (HCCS), transcript variant X1, mRNA.

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CloneID	OHu106260
Clone ID Related Accession (Same CDS sequence)	XM_024452368.1
Accession Version	XM_024452368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTA GTTCAGTTCT TGATAGATTT AAACAGAAGA AACCTGGCTT TTCCTATGGT 
GGTAATCATT GGTCAGAAAT TCAGCTCTTT AGTGCTCCAA TTGAGAGAGT TAGATGTCCT 
GCCTTCATGG TGACACCATT TTTATACTTG ATTATTTCAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308136.1
CDS	2832..3698
Translation	MLASSVLDRFKQKKPGFSYGGNHWSEIQLFSAPIERVRCPAFMVTPFLYLIISGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	XM_024452368.1
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452368.1

ORF Insert Sequence:

ATGTTAGCTA GTTCAGTTCT TGATAGATTT AAACAGAAGA AACCTGGCTT TTCCTATGGT 
GGTAATCATT GGTCAGAAAT TCAGCTCTTT AGTGCTCCAA TTGAGAGAGT TAGATGTCCT 
GCCTTCATGG TGACACCATT TTTATACTTG ATTATTTCAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_001171991.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165462.1 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_001171991.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB253108.1, BC001691.2, BC095455.1 and AI027533.1. On May 13, 2010 this sequence version replaced NM_001171991.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.118274.1, SRR1660803.128446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165462.1
CDS	181..987
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_001171991.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171991.2

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_005333.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005324.3 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_005333.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB253108.1, BC095455.1 and AI027533.1. On Nov 23, 2018 this sequence version replaced NM_005333.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749578.1, SRR3476690.279376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380762.5/ ENSP00000370139.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005324.3
CDS	230..1036
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_005333.5
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005333.5

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_001122608.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116080.1 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_001122608.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB253108.1, AK097815.1 and AI027533.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_001122608.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.639852.1, AK097815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116080.1
CDS	218..1024
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_001122608.2
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122608.2

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_001122608.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116080.1 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.

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CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_001122608.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569600000000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK097815.1 and AI027533.1. This sequence is a reference standard in the RefSeqGene project. On Jun 1, 2019 this sequence version replaced NM_001122608.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.10361.1, SRR1803615.149874.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116080.1
CDS	172..978
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_001122608.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122608.3

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_001171991.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165462.1 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.

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CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_001171991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB253108.1, BC001691.2, BC095455.1 and AI027533.1. On May 31, 2019 this sequence version replaced NM_001171991.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.21666.1, SRR1803611.118274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165462.1
CDS	135..941
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_001171991.3
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171991.3

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCCS ( NM_005333.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005324.3 Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.

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CloneID	OHu27077
Clone ID Related Accession (Same CDS sequence)	NM_001171991.2 , NM_005333.5 , NM_001122608.2 , NM_001122608.3 , NM_001171991.3 , NM_005333.4
Accession Version	NM_005333.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	cytochrome c-type heme lyase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB253108.1, BC095455.1 and AI027533.1. On May 13, 2010 this sequence version replaced NM_005333.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749578.1, SRR1660803.158545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005324.3
CDS	276..1082
Translation	MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Target ORF information:

RefSeq Version	NM_005333.4
Organism	Homo sapiens(human)
Definition	Homo sapiens holocytochrome c synthase (HCCS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005333.4

ORF Insert Sequence:

ATGGGTTTGT CTCCATCTGC TCCTGCTGTT GCAGTTCAGG CCTCAAATGC TTCAGCGTCC 
CCACCTTCAG GATGCCCGAT GCATGAAGGG AAAATGAAAG GCTGTCCAGT GAATACAGAG 
CCATCTGGCC CAACCTGTGA GAAGAAAACA TACTCTGTGC CTGCCCACCA GGAACGCGCC 
TATGAGTACG TGGAGTGTCC CATTAGGGGC ACTGCGGCTG AGAATAAGGA GAACCTAGAT 
CCTTCAAATC TGATGCCACC ACCAAATCAA ACACCAGCTC CAGATCAGCC ATTTGCATTG 
TCTACTGTCA GAGAAGAGTC ATCCATTCCG AGAGCAGATT CAGAGAAAAA GTGGGTTTAC 
CCTTCTGAGC AGATGTTCTG GAATGCAATG TTAAAGAAAG GGTGGAAGTG GAAGGATGAG 
GATATCAGTC AGAAGGATAT GTATAATATC ATTAGAATTC ACAATCAGAA TAACGAGCAG 
GCTTGGAAGG AGATTTTGAA GTGGGAAGCC CTTCATGCTG CAGAGTGTCC TTGTGGTCCA 
TCATTGATCC GGTTTGGAGG GAAAGCAAAA GAGTATTCAC CAAGGGCACG AATTCGTTCC 
TGGATGGGGT ATGAGTTGCC TTTTGATAGG CACGATTGGA TCATAAACCG TTGCGGGACA 
GAAGTTAGAT ATGTGATTGA TTATTATGAT GGTGGTGAAG TCAACAAGGA CTACCAGTTC 
ACCATCCTGG ACGTCCGTCC TGCCTTAGAT TCACTTTCGG CAGTATGGGA CAGAATGAAA 
GTCGCTTGGT GGCGTTGGAC CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biosynthesis of Single Thioether c-Type Cytochromes Provides Insight into Mechanisms Intrinsic to Holocytochrome c Synthase (HCCS).
Biochemistry56(26)3337-3346(2017 07)
Babbitt SE,Hsu J,Mendez DL,Kranz RG

Molecular Basis Behind Inability of Mitochondrial Holocytochrome c Synthase to Mature Bacterial Cytochromes: DEFINING A CRITICAL ROLE FOR CYTOCHROME c α HELIX-1.
The Journal of biological chemistry291(34)17523-34(2016 08)
Babbitt SE,Hsu J,Kranz RG

Mechanisms of mitochondrial holocytochrome c synthase and the key roles played by cysteines and histidine of the heme attachment site, Cys-XX-Cys-His.
The Journal of biological chemistry289(42)28795-807(2014 Oct)
Babbitt SE,San Francisco B,Mendez DL,Lukat-Rodgers GS,Rodgers KR,Bretsnyder EC,Kranz RG

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HCCS cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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