GTF2A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTF2A1
Entrez Gene ID	2957
Full Name	general transcription factor IIA subunit 1
Synonyms	TF2A1,TFIIA,TFIIA-42,TFIIAL
General protein information	Preferred Names general transcription factor IIA subunit 1 Names transcription initiation factor IIA subunit 1 TFIIA alpha, p55 TFIIA alpha/beta subunits general transcription factor IIA 1 general transcription factor IIA, 1, 19/37kDa glucose regulated protein, 58kD pseudogene transcription initiation factor TFIIA 42 kDa subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q31.1
Summary	Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010].
Disorder MIM:	600520

mRNA and Protein(s)

mRNA	Protein	Name
NM_015859.3	NP_056943.1	transcription initiation factor IIA subunit 1 isoform 1
NM_201595.3	NP_963889.1	transcription initiation factor IIA subunit 1 isoform 2
NM_001278940.1	NP_001265869.1	transcription initiation factor IIA subunit 1 isoform 3
NM_001278940.2	NP_001265869.1	transcription initiation factor IIA subunit 1 isoform 3
NM_201595.2	NP_963889.1	transcription initiation factor IIA subunit 1 isoform 2
NM_015859.4	NP_056943.1	transcription initiation factor IIA subunit 1 isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	GTF2A1	XP_001105362.1
Bos taurus (cattle)	GTF2A1	NP_001178146.2
Gallus gallus (chicken)	GTF2A1	NP_001026378.2
Canis lupus familiaris (dog)	GTF2A1	XP_005623775.1
Mus musculus (house mouse)	Gtf2a1	NP_113568.2
Rattus norvegicus (Norway rat)	Gtf2a1	NP_071544.1
Homo sapiens (human)	GTF2A1	NP_056943.1
Pan troglodytes (chimpanzee)	GTF2A1	XP_001134883.1
Danio rerio (zebrafish)	gtf2a1	NP_997822.1
Xenopus tropicalis (tropical clawed frog)	gtf2a1	NP_001016908.1

Gene Ontology
Bibliography

Related articles in PubMed

TFIIA transcriptional activity is controlled by a 'cleave-and-run' Exportin-1/Taspase 1-switch.
Schrenk C, Fetz V, Vallet C, Heiselmayer C, Schröder E, Hensel A, Hahlbrock A, Wünsch D, Goesswein D, Bier C, Habtemichael N, Schneider G, Stauber RH, Knauer SK
Journal of molecular cell biology10(1)33-47(2018 02)

Disease-relevant signalling-pathways in head and neck cancer: Taspase1's proteolytic activity fine-tunes TFIIA function.
Gribko A, Hahlbrock A, Strieth S, Becker S, Hagemann J, Deichelbohrer M, Hildebrandt A, Habtemichael N, Wünsch D
Scientific reports7(1)14937(2017 11)

A transcription factor IIA-binding site differentially regulates RNA polymerase II-mediated transcription in a promoter context-dependent manner.
Wang J, Zhao S, He W, Wei Y, Zhang Y, Pegg H, Shore P, Roberts SGE, Deng W
The Journal of biological chemistry292(28)11873-11885(2017 07)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Integrated transcriptome and methylome analysis in youth at high risk for bipolar disorder: a preliminary analysis.
Fries GR, Quevedo J, Zeni CP, Kazimi IF, Zunta-Soares G, Spiker DE, Bowden CL, Walss-Bass C, Soares JC
Translational psychiatry7(3)e1059(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GTF2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07922	NM_001278940.2 Latest version!	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OHu12699	NM_015859.4 Latest version!	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
OHu12740	NM_201595.3 Latest version!	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu07922	NM_001278940.1	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OHu12740	NM_201595.2	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu12699	NM_015859.3	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTF2A1 ( NM_001278940.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265869.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07922
Clone ID Related Accession (Same CDS sequence)	NM_001278940.1 , NM_001278940.2
Accession Version	NM_001278940.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532102400000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050703.1, BC107156.1 and AL136040.5. On Jul 18, 2013 this sequence version replaced NR_048573.1. Transcript Variant: This variant (3) includes an alternate exon and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050703.1, SRR1803611.230471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTCCA GGGCAGTAGA TGGATTTCAT TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT 
CAACAGCAGC ATCAACCCCA GCAGCAGCAG CATCACCACC ATCACCATCA TCAGCAAGCT 
CAGCCTCAGC AGACAGTACC TCAGCAAGCG CAGACCCAGC AGGTTCTTAT TCCTGCATCA 
CAGCAAGCCA CAGCACCACA AGTTATTGTT CCAGATTCTA AGTTGATACA GCATATGAAT 
GCATCAAACA TGAGTGCTGC TGCTACAGCT GCTACCTTAG CACTCCCTGC AGGTGTGACT 
CCTGTTCAGC AGATATTAAC AAATTCAGGC CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT 
GGTGCCCAAT ATATCTTTCA GCCTCAGCAG TCAGTGGTTC TACAACAACA GGTTATACCA 
CAAATGCAGC CTGGTGGAGT ACAAGCTCCT GTTATACAGC AGGTGCTGGC TCCTCTTCCT 
GGAGGGATTT CACCACAGAC AGGTGTCATC ATCCAGCCTC AGCAAATCTT ATTTACAGGA 
AATAAGACTC AAGTTATACC TACGACAGTG GCAGCACCTA CACCAGCCCA AGCACAGATA 
ACTGCAACTG GCCAGCAGCA ACCGCAGGCC CAGCCTGCTC AAACACAAGC TCCATTGGTC 
TTACAAGTTG ATGGAACTGG GGATACATCA TCTGAAGAAG ATGAAGATGA AGAAGAAGAC 
TATGATGATG ATGAGGAGGA AGACAAAGAG AAAGATGGAG CTGAAGATGG GCAGGTGGAA 
GAAGAGCCCC TCAATAGTGA AGATGATGTG AGTGATGAGG AAGGACAGGA ACTCTTTGAC 
ACAGAAAATG TTGTTGTATG CCAATATGAT AAGATACACA GAAGTAAAAA CAAATGGAAA 
TTTCATCTCA AGGATGGCAT TATGAATCTT AATGGAAGAG ATTATATATT TTCCAAAGCC 
ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265869.1
CDS	652..1632
Translation	MQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_001278940.1
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278940.1

ORF Insert Sequence:

ATGCAGTCCA GGGCAGTAGA TGGATTTCAT TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT 
CAACAGCAGC ATCAACCCCA GCAGCAGCAG CATCACCACC ATCACCATCA TCAGCAAGCT 
CAGCCTCAGC AGACAGTACC TCAGCAAGCG CAGACCCAGC AGGTTCTTAT TCCTGCATCA 
CAGCAAGCCA CAGCACCACA AGTTATTGTT CCAGATTCTA AGTTGATACA GCATATGAAT 
GCATCAAACA TGAGTGCTGC TGCTACAGCT GCTACCTTAG CACTCCCTGC AGGTGTGACT 
CCTGTTCAGC AGATATTAAC AAATTCAGGC CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT 
GGTGCCCAAT ATATCTTTCA GCCTCAGCAG TCAGTGGTTC TACAACAACA GGTTATACCA 
CAAATGCAGC CTGGTGGAGT ACAAGCTCCT GTTATACAGC AGGTGCTGGC TCCTCTTCCT 
GGAGGGATTT CACCACAGAC AGGTGTCATC ATCCAGCCTC AGCAAATCTT ATTTACAGGA 
AATAAGACTC AAGTTATACC TACGACAGTG GCAGCACCTA CACCAGCCCA AGCACAGATA 
ACTGCAACTG GCCAGCAGCA ACCGCAGGCC CAGCCTGCTC AAACACAAGC TCCATTGGTC 
TTACAAGTTG ATGGAACTGG GGATACATCA TCTGAAGAAG ATGAAGATGA AGAAGAAGAC 
TATGATGATG ATGAGGAGGA AGACAAAGAG AAAGATGGAG CTGAAGATGG GCAGGTGGAA 
GAAGAGCCCC TCAATAGTGA AGATGATGTG AGTGATGAGG AAGGACAGGA ACTCTTTGAC 
ACAGAAAATG TTGTTGTATG CCAATATGAT AAGATACACA GAAGTAAAAA CAAATGGAAA 
TTTCATCTCA AGGATGGCAT TATGAATCTT AATGGAAGAG ATTATATATT TTCCAAAGCC 
ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2A1 ( NM_001278940.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265869.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07922
Clone ID Related Accession (Same CDS sequence)	NM_001278940.1 , NM_001278940.2
Accession Version	NM_001278940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL136040.5, BC050703.1 and BC107156.1. On May 31, 2019 this sequence version replaced NM_001278940.1. Transcript Variant: This variant (3) includes an alternate exon and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.230471.1, BC050703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTCCA GGGCAGTAGA TGGATTTCAT TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT 
CAACAGCAGC ATCAACCCCA GCAGCAGCAG CATCACCACC ATCACCATCA TCAGCAAGCT 
CAGCCTCAGC AGACAGTACC TCAGCAAGCG CAGACCCAGC AGGTTCTTAT TCCTGCATCA 
CAGCAAGCCA CAGCACCACA AGTTATTGTT CCAGATTCTA AGTTGATACA GCATATGAAT 
GCATCAAACA TGAGTGCTGC TGCTACAGCT GCTACCTTAG CACTCCCTGC AGGTGTGACT 
CCTGTTCAGC AGATATTAAC AAATTCAGGC CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT 
GGTGCCCAAT ATATCTTTCA GCCTCAGCAG TCAGTGGTTC TACAACAACA GGTTATACCA 
CAAATGCAGC CTGGTGGAGT ACAAGCTCCT GTTATACAGC AGGTGCTGGC TCCTCTTCCT 
GGAGGGATTT CACCACAGAC AGGTGTCATC ATCCAGCCTC AGCAAATCTT ATTTACAGGA 
AATAAGACTC AAGTTATACC TACGACAGTG GCAGCACCTA CACCAGCCCA AGCACAGATA 
ACTGCAACTG GCCAGCAGCA ACCGCAGGCC CAGCCTGCTC AAACACAAGC TCCATTGGTC 
TTACAAGTTG ATGGAACTGG GGATACATCA TCTGAAGAAG ATGAAGATGA AGAAGAAGAC 
TATGATGATG ATGAGGAGGA AGACAAAGAG AAAGATGGAG CTGAAGATGG GCAGGTGGAA 
GAAGAGCCCC TCAATAGTGA AGATGATGTG AGTGATGAGG AAGGACAGGA ACTCTTTGAC 
ACAGAAAATG TTGTTGTATG CCAATATGAT AAGATACACA GAAGTAAAAA CAAATGGAAA 
TTTCATCTCA AGGATGGCAT TATGAATCTT AATGGAAGAG ATTATATATT TTCCAAAGCC 
ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265869.1
CDS	659..1639
Translation	MQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_001278940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278940.2

ORF Insert Sequence:

ATGCAGTCCA GGGCAGTAGA TGGATTTCAT TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT 
CAACAGCAGC ATCAACCCCA GCAGCAGCAG CATCACCACC ATCACCATCA TCAGCAAGCT 
CAGCCTCAGC AGACAGTACC TCAGCAAGCG CAGACCCAGC AGGTTCTTAT TCCTGCATCA 
CAGCAAGCCA CAGCACCACA AGTTATTGTT CCAGATTCTA AGTTGATACA GCATATGAAT 
GCATCAAACA TGAGTGCTGC TGCTACAGCT GCTACCTTAG CACTCCCTGC AGGTGTGACT 
CCTGTTCAGC AGATATTAAC AAATTCAGGC CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT 
GGTGCCCAAT ATATCTTTCA GCCTCAGCAG TCAGTGGTTC TACAACAACA GGTTATACCA 
CAAATGCAGC CTGGTGGAGT ACAAGCTCCT GTTATACAGC AGGTGCTGGC TCCTCTTCCT 
GGAGGGATTT CACCACAGAC AGGTGTCATC ATCCAGCCTC AGCAAATCTT ATTTACAGGA 
AATAAGACTC AAGTTATACC TACGACAGTG GCAGCACCTA CACCAGCCCA AGCACAGATA 
ACTGCAACTG GCCAGCAGCA ACCGCAGGCC CAGCCTGCTC AAACACAAGC TCCATTGGTC 
TTACAAGTTG ATGGAACTGG GGATACATCA TCTGAAGAAG ATGAAGATGA AGAAGAAGAC 
TATGATGATG ATGAGGAGGA AGACAAAGAG AAAGATGGAG CTGAAGATGG GCAGGTGGAA 
GAAGAGCCCC TCAATAGTGA AGATGATGTG AGTGATGAGG AAGGACAGGA ACTCTTTGAC 
ACAGAAAATG TTGTTGTATG CCAATATGAT AAGATACACA GAAGTAAAAA CAAATGGAAA 
TTTCATCTCA AGGATGGCAT TATGAATCTT AATGGAAGAG ATTATATATT TTCCAAAGCC 
ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2A1 ( NM_015859.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056943.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12699
Clone ID Related Accession (Same CDS sequence)	NM_015859.3 , NM_015859.4
Accession Version	NM_015859.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050703.1, D14887.1 and AL136040.5. On May 16, 2012 this sequence version replaced NM_015859.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.69478.1, SRR1660803.253313.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACT CGGCAAATAC AAACACCGTG CCTAAATTAT ACAGATCTGT GATTGAAGAT 
GTCATTAATG ATGTGAGAGA CATCTTTCTG GATGATGGAG TGGATGAACA AGTACTGATG 
GAACTAAAAA CTTTATGGGA AAACAAACTA ATGCAGTCCA GGGCAGTAGA TGGATTTCAT 
TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT CAACAGCAGC ATCAACCCCA GCAGCAGCAG 
CATCACCACC ATCACCATCA TCAGCAAGCT CAGCCTCAGC AGACAGTACC TCAGCAAGCG 
CAGACCCAGC AGGTTCTTAT TCCTGCATCA CAGCAAGCCA CAGCACCACA AGTTATTGTT 
CCAGATTCTA AGTTGATACA GCATATGAAT GCATCAAACA TGAGTGCTGC TGCTACAGCT 
GCTACCTTAG CACTCCCTGC AGGTGTGACT CCTGTTCAGC AGATATTAAC AAATTCAGGC 
CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT GGTGCCCAAT ATATCTTTCA GCCTCAGCAG 
TCAGTGGTTC TACAACAACA GGTTATACCA CAAATGCAGC CTGGTGGAGT ACAAGCTCCT 
GTTATACAGC AGGTGCTGGC TCCTCTTCCT GGAGGGATTT CACCACAGAC AGGTGTCATC 
ATCCAGCCTC AGCAAATCTT ATTTACAGGA AATAAGACTC AAGTTATACC TACGACAGTG 
GCAGCACCTA CACCAGCCCA AGCACAGATA ACTGCAACTG GCCAGCAGCA ACCGCAGGCC 
CAGCCTGCTC AAACACAAGC TCCATTGGTC TTACAAGTTG ATGGAACTGG GGATACATCA 
TCTGAAGAAG ATGAAGATGA AGAAGAAGAC TATGATGATG ATGAGGAGGA AGACAAAGAG 
AAAGATGGAG CTGAAGATGG GCAGGTGGAA GAAGAGCCCC TCAATAGTGA AGATGATGTG 
AGTGATGAGG AAGGACAGGA ACTCTTTGAC ACAGAAAATG TTGTTGTATG CCAATATGAT 
AAGATACACA GAAGTAAAAA CAAATGGAAA TTTCATCTCA AGGATGGCAT TATGAATCTT 
AATGGAAGAG ATTATATATT TTCCAAAGCC ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056943.1
CDS	435..1565
Translation	MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_015859.3
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015859.3

ORF Insert Sequence:

ATGGCGAACT CGGCAAATAC AAACACCGTG CCTAAATTAT ACAGATCTGT GATTGAAGAT 
GTCATTAATG ATGTGAGAGA CATCTTTCTG GATGATGGAG TGGATGAACA AGTACTGATG 
GAACTAAAAA CTTTATGGGA AAACAAACTA ATGCAGTCCA GGGCAGTAGA TGGATTTCAT 
TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT CAACAGCAGC ATCAACCCCA GCAGCAGCAG 
CATCACCACC ATCACCATCA TCAGCAAGCT CAGCCTCAGC AGACAGTACC TCAGCAAGCG 
CAGACCCAGC AGGTTCTTAT TCCTGCATCA CAGCAAGCCA CAGCACCACA AGTTATTGTT 
CCAGATTCTA AGTTGATACA GCATATGAAT GCATCAAACA TGAGTGCTGC TGCTACAGCT 
GCTACCTTAG CACTCCCTGC AGGTGTGACT CCTGTTCAGC AGATATTAAC AAATTCAGGC 
CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT GGTGCCCAAT ATATCTTTCA GCCTCAGCAG 
TCAGTGGTTC TACAACAACA GGTTATACCA CAAATGCAGC CTGGTGGAGT ACAAGCTCCT 
GTTATACAGC AGGTGCTGGC TCCTCTTCCT GGAGGGATTT CACCACAGAC AGGTGTCATC 
ATCCAGCCTC AGCAAATCTT ATTTACAGGA AATAAGACTC AAGTTATACC TACGACAGTG 
GCAGCACCTA CACCAGCCCA AGCACAGATA ACTGCAACTG GCCAGCAGCA ACCGCAGGCC 
CAGCCTGCTC AAACACAAGC TCCATTGGTC TTACAAGTTG ATGGAACTGG GGATACATCA 
TCTGAAGAAG ATGAAGATGA AGAAGAAGAC TATGATGATG ATGAGGAGGA AGACAAAGAG 
AAAGATGGAG CTGAAGATGG GCAGGTGGAA GAAGAGCCCC TCAATAGTGA AGATGATGTG 
AGTGATGAGG AAGGACAGGA ACTCTTTGAC ACAGAAAATG TTGTTGTATG CCAATATGAT 
AAGATACACA GAAGTAAAAA CAAATGGAAA TTTCATCTCA AGGATGGCAT TATGAATCTT 
AATGGAAGAG ATTATATATT TTCCAAAGCC ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2A1 ( NM_015859.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056943.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12699
Clone ID Related Accession (Same CDS sequence)	NM_015859.3 , NM_015859.4
Accession Version	NM_015859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL136040.5, BC050703.1 and D14887.1. On Nov 22, 2018 this sequence version replaced NM_015859.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.69478.1, SRR1660803.253313.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000553612.6/ ENSP00000452454.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAACT CGGCAAATAC AAACACCGTG CCTAAATTAT ACAGATCTGT GATTGAAGAT 
GTCATTAATG ATGTGAGAGA CATCTTTCTG GATGATGGAG TGGATGAACA AGTACTGATG 
GAACTAAAAA CTTTATGGGA AAACAAACTA ATGCAGTCCA GGGCAGTAGA TGGATTTCAT 
TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT CAACAGCAGC ATCAACCCCA GCAGCAGCAG 
CATCACCACC ATCACCATCA TCAGCAAGCT CAGCCTCAGC AGACAGTACC TCAGCAAGCG 
CAGACCCAGC AGGTTCTTAT TCCTGCATCA CAGCAAGCCA CAGCACCACA AGTTATTGTT 
CCAGATTCTA AGTTGATACA GCATATGAAT GCATCAAACA TGAGTGCTGC TGCTACAGCT 
GCTACCTTAG CACTCCCTGC AGGTGTGACT CCTGTTCAGC AGATATTAAC AAATTCAGGC 
CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT GGTGCCCAAT ATATCTTTCA GCCTCAGCAG 
TCAGTGGTTC TACAACAACA GGTTATACCA CAAATGCAGC CTGGTGGAGT ACAAGCTCCT 
GTTATACAGC AGGTGCTGGC TCCTCTTCCT GGAGGGATTT CACCACAGAC AGGTGTCATC 
ATCCAGCCTC AGCAAATCTT ATTTACAGGA AATAAGACTC AAGTTATACC TACGACAGTG 
GCAGCACCTA CACCAGCCCA AGCACAGATA ACTGCAACTG GCCAGCAGCA ACCGCAGGCC 
CAGCCTGCTC AAACACAAGC TCCATTGGTC TTACAAGTTG ATGGAACTGG GGATACATCA 
TCTGAAGAAG ATGAAGATGA AGAAGAAGAC TATGATGATG ATGAGGAGGA AGACAAAGAG 
AAAGATGGAG CTGAAGATGG GCAGGTGGAA GAAGAGCCCC TCAATAGTGA AGATGATGTG 
AGTGATGAGG AAGGACAGGA ACTCTTTGAC ACAGAAAATG TTGTTGTATG CCAATATGAT 
AAGATACACA GAAGTAAAAA CAAATGGAAA TTTCATCTCA AGGATGGCAT TATGAATCTT 
AATGGAAGAG ATTATATATT TTCCAAAGCC ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056943.1
CDS	442..1572
Translation	MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_015859.4
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015859.4

ORF Insert Sequence:

ATGGCGAACT CGGCAAATAC AAACACCGTG CCTAAATTAT ACAGATCTGT GATTGAAGAT 
GTCATTAATG ATGTGAGAGA CATCTTTCTG GATGATGGAG TGGATGAACA AGTACTGATG 
GAACTAAAAA CTTTATGGGA AAACAAACTA ATGCAGTCCA GGGCAGTAGA TGGATTTCAT 
TCAGAAGAGC AGCAGCTTCT ACTGCAAGTT CAACAGCAGC ATCAACCCCA GCAGCAGCAG 
CATCACCACC ATCACCATCA TCAGCAAGCT CAGCCTCAGC AGACAGTACC TCAGCAAGCG 
CAGACCCAGC AGGTTCTTAT TCCTGCATCA CAGCAAGCCA CAGCACCACA AGTTATTGTT 
CCAGATTCTA AGTTGATACA GCATATGAAT GCATCAAACA TGAGTGCTGC TGCTACAGCT 
GCTACCTTAG CACTCCCTGC AGGTGTGACT CCTGTTCAGC AGATATTAAC AAATTCAGGC 
CAGCTTCTTC AGGTGGTCAG AGCAGCCAAT GGTGCCCAAT ATATCTTTCA GCCTCAGCAG 
TCAGTGGTTC TACAACAACA GGTTATACCA CAAATGCAGC CTGGTGGAGT ACAAGCTCCT 
GTTATACAGC AGGTGCTGGC TCCTCTTCCT GGAGGGATTT CACCACAGAC AGGTGTCATC 
ATCCAGCCTC AGCAAATCTT ATTTACAGGA AATAAGACTC AAGTTATACC TACGACAGTG 
GCAGCACCTA CACCAGCCCA AGCACAGATA ACTGCAACTG GCCAGCAGCA ACCGCAGGCC 
CAGCCTGCTC AAACACAAGC TCCATTGGTC TTACAAGTTG ATGGAACTGG GGATACATCA 
TCTGAAGAAG ATGAAGATGA AGAAGAAGAC TATGATGATG ATGAGGAGGA AGACAAAGAG 
AAAGATGGAG CTGAAGATGG GCAGGTGGAA GAAGAGCCCC TCAATAGTGA AGATGATGTG 
AGTGATGAGG AAGGACAGGA ACTCTTTGAC ACAGAAAATG TTGTTGTATG CCAATATGAT 
AAGATACACA GAAGTAAAAA CAAATGGAAA TTTCATCTCA AGGATGGCAT TATGAATCTT 
AATGGAAGAG ATTATATATT TTCCAAAGCC ATTGGAGATG CAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2A1 ( NM_201595.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_963889.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12740
Clone ID Related Accession (Same CDS sequence)	NM_201595.3 , NM_201595.2
Accession Version	NM_201595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL136040.5 and D14886.1. On May 31, 2019 this sequence version replaced NM_201595.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D14886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTAA AAACTTTATG GGAAAACAAA CTAATGCAGT CCAGGGCAGT AGATGGATTT 
CATTCAGAAG AGCAGCAGCT TCTACTGCAA GTTCAACAGC AGCATCAACC CCAGCAGCAG 
CAGCATCACC ACCATCACCA TCATCAGCAA GCTCAGCCTC AGCAGACAGT ACCTCAGCAA 
GCGCAGACCC AGCAGGTTCT TATTCCTGCA TCACAGCAAG CCACAGCACC ACAAGTTATT 
GTTCCAGATT CTAAGTTGAT ACAGCATATG AATGCATCAA ACATGAGTGC TGCTGCTACA 
GCTGCTACCT TAGCACTCCC TGCAGGTGTG ACTCCTGTTC AGCAGATATT AACAAATTCA 
GGCCAGCTTC TTCAGGTGGT CAGAGCAGCC AATGGTGCCC AATATATCTT TCAGCCTCAG 
CAGTCAGTGG TTCTACAACA ACAGGTTATA CCACAAATGC AGCCTGGTGG AGTACAAGCT 
CCTGTTATAC AGCAGGTGCT GGCTCCTCTT CCTGGAGGGA TTTCACCACA GACAGGTGTC 
ATCATCCAGC CTCAGCAAAT CTTATTTACA GGAAATAAGA CTCAAGTTAT ACCTACGACA 
GTGGCAGCAC CTACACCAGC CCAAGCACAG ATAACTGCAA CTGGCCAGCA GCAACCGCAG 
GCCCAGCCTG CTCAAACACA AGCTCCATTG GTCTTACAAG TTGATGGAAC TGGGGATACA 
TCATCTGAAG AAGATGAAGA TGAAGAAGAA GACTATGATG ATGATGAGGA GGAAGACAAA 
GAGAAAGATG GAGCTGAAGA TGGGCAGGTG GAAGAAGAGC CCCTCAATAG TGAAGATGAT 
GTGAGTGATG AGGAAGGACA GGAACTCTTT GACACAGAAA ATGTTGTTGT ATGCCAATAT 
GATAAGATAC ACAGAAGTAA AAACAAATGG AAATTTCATC TCAAGGATGG CATTATGAAT 
CTTAATGGAA GAGATTATAT ATTTTCCAAA GCCATTGGAG ATGCAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963889.1
CDS	331..1344
Translation	MELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_201595.3
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201595.3

ORF Insert Sequence:

ATGGAACTAA AAACTTTATG GGAAAACAAA CTAATGCAGT CCAGGGCAGT AGATGGATTT 
CATTCAGAAG AGCAGCAGCT TCTACTGCAA GTTCAACAGC AGCATCAACC CCAGCAGCAG 
CAGCATCACC ACCATCACCA TCATCAGCAA GCTCAGCCTC AGCAGACAGT ACCTCAGCAA 
GCGCAGACCC AGCAGGTTCT TATTCCTGCA TCACAGCAAG CCACAGCACC ACAAGTTATT 
GTTCCAGATT CTAAGTTGAT ACAGCATATG AATGCATCAA ACATGAGTGC TGCTGCTACA 
GCTGCTACCT TAGCACTCCC TGCAGGTGTG ACTCCTGTTC AGCAGATATT AACAAATTCA 
GGCCAGCTTC TTCAGGTGGT CAGAGCAGCC AATGGTGCCC AATATATCTT TCAGCCTCAG 
CAGTCAGTGG TTCTACAACA ACAGGTTATA CCACAAATGC AGCCTGGTGG AGTACAAGCT 
CCTGTTATAC AGCAGGTGCT GGCTCCTCTT CCTGGAGGGA TTTCACCACA GACAGGTGTC 
ATCATCCAGC CTCAGCAAAT CTTATTTACA GGAAATAAGA CTCAAGTTAT ACCTACGACA 
GTGGCAGCAC CTACACCAGC CCAAGCACAG ATAACTGCAA CTGGCCAGCA GCAACCGCAG 
GCCCAGCCTG CTCAAACACA AGCTCCATTG GTCTTACAAG TTGATGGAAC TGGGGATACA 
TCATCTGAAG AAGATGAAGA TGAAGAAGAA GACTATGATG ATGATGAGGA GGAAGACAAA 
GAGAAAGATG GAGCTGAAGA TGGGCAGGTG GAAGAAGAGC CCCTCAATAG TGAAGATGAT 
GTGAGTGATG AGGAAGGACA GGAACTCTTT GACACAGAAA ATGTTGTTGT ATGCCAATAT 
GATAAGATAC ACAGAAGTAA AAACAAATGG AAATTTCATC TCAAGGATGG CATTATGAAT 
CTTAATGGAA GAGATTATAT ATTTTCCAAA GCCATTGGAG ATGCAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTF2A1 ( NM_201595.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_963889.1 Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12740
Clone ID Related Accession (Same CDS sequence)	NM_201595.3 , NM_201595.2
Accession Version	NM_201595.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532102400000
Organism	Homo sapiens(human)
Product	transcription initiation factor IIA subunit 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D14886.1 and AL136040.5. On May 16, 2012 this sequence version replaced NM_201595.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D14886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTAA AAACTTTATG GGAAAACAAA CTAATGCAGT CCAGGGCAGT AGATGGATTT 
CATTCAGAAG AGCAGCAGCT TCTACTGCAA GTTCAACAGC AGCATCAACC CCAGCAGCAG 
CAGCATCACC ACCATCACCA TCATCAGCAA GCTCAGCCTC AGCAGACAGT ACCTCAGCAA 
GCGCAGACCC AGCAGGTTCT TATTCCTGCA TCACAGCAAG CCACAGCACC ACAAGTTATT 
GTTCCAGATT CTAAGTTGAT ACAGCATATG AATGCATCAA ACATGAGTGC TGCTGCTACA 
GCTGCTACCT TAGCACTCCC TGCAGGTGTG ACTCCTGTTC AGCAGATATT AACAAATTCA 
GGCCAGCTTC TTCAGGTGGT CAGAGCAGCC AATGGTGCCC AATATATCTT TCAGCCTCAG 
CAGTCAGTGG TTCTACAACA ACAGGTTATA CCACAAATGC AGCCTGGTGG AGTACAAGCT 
CCTGTTATAC AGCAGGTGCT GGCTCCTCTT CCTGGAGGGA TTTCACCACA GACAGGTGTC 
ATCATCCAGC CTCAGCAAAT CTTATTTACA GGAAATAAGA CTCAAGTTAT ACCTACGACA 
GTGGCAGCAC CTACACCAGC CCAAGCACAG ATAACTGCAA CTGGCCAGCA GCAACCGCAG 
GCCCAGCCTG CTCAAACACA AGCTCCATTG GTCTTACAAG TTGATGGAAC TGGGGATACA 
TCATCTGAAG AAGATGAAGA TGAAGAAGAA GACTATGATG ATGATGAGGA GGAAGACAAA 
GAGAAAGATG GAGCTGAAGA TGGGCAGGTG GAAGAAGAGC CCCTCAATAG TGAAGATGAT 
GTGAGTGATG AGGAAGGACA GGAACTCTTT GACACAGAAA ATGTTGTTGT ATGCCAATAT 
GATAAGATAC ACAGAAGTAA AAACAAATGG AAATTTCATC TCAAGGATGG CATTATGAAT 
CTTAATGGAA GAGATTATAT ATTTTCCAAA GCCATTGGAG ATGCAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963889.1
CDS	172..1185
Translation	MELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW

Target ORF information:

RefSeq Version	NM_201595.2
Organism	Homo sapiens(human)
Definition	Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201595.2

ORF Insert Sequence:

ATGGAACTAA AAACTTTATG GGAAAACAAA CTAATGCAGT CCAGGGCAGT AGATGGATTT 
CATTCAGAAG AGCAGCAGCT TCTACTGCAA GTTCAACAGC AGCATCAACC CCAGCAGCAG 
CAGCATCACC ACCATCACCA TCATCAGCAA GCTCAGCCTC AGCAGACAGT ACCTCAGCAA 
GCGCAGACCC AGCAGGTTCT TATTCCTGCA TCACAGCAAG CCACAGCACC ACAAGTTATT 
GTTCCAGATT CTAAGTTGAT ACAGCATATG AATGCATCAA ACATGAGTGC TGCTGCTACA 
GCTGCTACCT TAGCACTCCC TGCAGGTGTG ACTCCTGTTC AGCAGATATT AACAAATTCA 
GGCCAGCTTC TTCAGGTGGT CAGAGCAGCC AATGGTGCCC AATATATCTT TCAGCCTCAG 
CAGTCAGTGG TTCTACAACA ACAGGTTATA CCACAAATGC AGCCTGGTGG AGTACAAGCT 
CCTGTTATAC AGCAGGTGCT GGCTCCTCTT CCTGGAGGGA TTTCACCACA GACAGGTGTC 
ATCATCCAGC CTCAGCAAAT CTTATTTACA GGAAATAAGA CTCAAGTTAT ACCTACGACA 
GTGGCAGCAC CTACACCAGC CCAAGCACAG ATAACTGCAA CTGGCCAGCA GCAACCGCAG 
GCCCAGCCTG CTCAAACACA AGCTCCATTG GTCTTACAAG TTGATGGAAC TGGGGATACA 
TCATCTGAAG AAGATGAAGA TGAAGAAGAA GACTATGATG ATGATGAGGA GGAAGACAAA 
GAGAAAGATG GAGCTGAAGA TGGGCAGGTG GAAGAAGAGC CCCTCAATAG TGAAGATGAT 
GTGAGTGATG AGGAAGGACA GGAACTCTTT GACACAGAAA ATGTTGTTGT ATGCCAATAT 
GATAAGATAC ACAGAAGTAA AAACAAATGG AAATTTCATC TCAAGGATGG CATTATGAAT 
CTTAATGGAA GAGATTATAT ATTTTCCAAA GCCATTGGAG ATGCAGAATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TFIIA transcriptional activity is controlled by a 'cleave-and-run' Exportin-1/Taspase 1-switch.
Journal of molecular cell biology10(1)33-47(2018 02)
Schrenk C,Fetz V,Vallet C,Heiselmayer C,Schröder E,Hensel A,Hahlbrock A,Wünsch D,Goesswein D,Bier C,Habtemichael N,Schneider G,Stauber RH,Knauer SK

Disease-relevant signalling-pathways in head and neck cancer: Taspase1's proteolytic activity fine-tunes TFIIA function.
Scientific reports7(1)14937(2017 11)
Gribko A,Hahlbrock A,Strieth S,Becker S,Hagemann J,Deichelbohrer M,Hildebrandt A,Habtemichael N,Wünsch D

A transcription factor IIA-binding site differentially regulates RNA polymerase II-mediated transcription in a promoter context-dependent manner.
The Journal of biological chemistry292(28)11873-11885(2017 07)
Wang J,Zhao S,He W,Wei Y,Zhang Y,Pegg H,Shore P,Roberts SGE,Deng W

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Integrated transcriptome and methylome analysis in youth at high risk for bipolar disorder: a preliminary analysis.
Translational psychiatry7(3)e1059(2017 03)
Fries GR,Quevedo J,Zeni CP,Kazimi IF,Zunta-Soares G,Spiker DE,Bowden CL,Walss-Bass C,Soares JC

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GTF2A1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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