| Gene Symbol | BBC3 |
| Entrez Gene ID | 27113 |
| Full Name | BCL2 binding component 3 |
| Synonyms | JFY-1,JFY1,PUMA |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]. |
| Disorder MIM: |
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BBC3 cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following BBC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BBC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu19976 Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2 Accession Version NM_001127240.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1578067200000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127240.2. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120712.1 CDS 66..851 Translation 
Target ORF information:
RefSeq Version NM_001127240.3 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_001127240.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu19976 Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2 Accession Version NM_001127240.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1551542400000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354654.1, BG258126.1, U82987.1 and AA887822.1. This sequence is a reference standard in the RefSeqGene project. On Dec 30, 2011 this sequence version replaced NM_001127240.1. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120712.1 CDS 165..950 Translation
Target ORF information:
RefSeq Version NM_001127240.2 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001127240.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu20051 Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3 Accession Version NM_001127242.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1551542400000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 3 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354656.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127242.1. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120714.1 CDS 165..470 Translation
Target ORF information:
RefSeq Version NM_001127242.2 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001127242.2
ORF Insert Sequence:
1
61
121
181
241
301ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu20051 Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3 Accession Version NM_001127242.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1578067200000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 3 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127242.2. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120714.1 CDS 66..371 Translation
Target ORF information:
RefSeq Version NM_001127242.3 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001127242.3
ORF Insert Sequence:
1
61
121
181
241
301ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
ATGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu20134 Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2 Accession Version NM_001127241.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 396bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1577980800000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127241.2. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120713.1 CDS 66..461 Translation
Target ORF information:
RefSeq Version NM_001127241.3 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001127241.3
ORF Insert Sequence:
1
61
121
181
241
301
361ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu20134 Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2 Accession Version NM_001127241.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 396bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1551542400000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354655.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127241.1. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001120713.1 CDS 165..560 Translation
Target ORF information:
RefSeq Version NM_001127241.2 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001127241.2
ORF Insert Sequence:
1
61
121
181
241
301
361ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu67065 Clone ID Related Accession (Same CDS sequence) XM_011526722.2 Accession Version XM_011526722.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA
CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC
TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG
GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC
CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG
CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG
GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG
GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG
GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC
CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC
CACAGAGCCC CCGAGATGGA GCCCAATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_011525024.1 CDS 126..755 Translation
Target ORF information:
RefSeq Version XM_011526722.2 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_011526722.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA
CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC
TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG
GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC
CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG
CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG
GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG
GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG
GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC
CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC
CACAGAGCCC CCGAGATGGA GCCCAATTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu19952 Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4 Accession Version NM_014417.5 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1576684800000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 4 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014417.4. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279869.944.1, DRR138517.347682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_055232.1 CDS 287..868 Translation
Target ORF information:
RefSeq Version NM_014417.5 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_014417.5
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu19952 Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4 Accession Version NM_014417.4 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1551542400000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform 4 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332558.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_014417.3. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF332558.1, ERR279869.944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_055232.1 CDS 282..863 Translation
Target ORF information:
RefSeq Version NM_014417.4 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_014417.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu19952 Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4 Accession Version XM_006723141.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product bcl-2-binding component 3 isoform X2 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006723204.1 CDS 233..814 Translation
Target ORF information:
RefSeq Version XM_006723141.3 Organism Homo sapiens(human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006723141.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu19952 Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4 Accession Version XM_006723141.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1426003200000 Organism Homo sapiens(Human) Product bcl-2-binding component 3 isoform X1 Comment
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_006723204.1 Translation
Target ORF information:
RefSeq Version XM_006723141.2 Organism Homo sapiens(Human) Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_006723141.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
-
PubMed
Wild-Type p53 Promotes Cancer Metabolic Switch by Inducing PUMA-Dependent Suppression of Oxidative Phosphorylation.
Cancer cell35(2)191-203.e8(2019 02)
Kim J,Yu L,Chen W,Xu Y,Wu M,Todorova D,Tang Q,Feng B,Jiang L,He J,Chen G,Fu X,Xu Y
Long intergenic noncoding RNA-p21 inhibits apoptosis by decreasing PUMA expression in non-small cell lung cancer.
The Journal of international medical research47(1)481-493(2019 Jan)
Yang T,Zhang W,Wang L,Xiao C,Guo B,Gong Y,Liang X,Huang D,Li Q,Nan Y,Xiang Y,Shao J
Tamoxifen overrides autophagy inhibition in Beclin-1-deficient glioma cells and their resistance to adenovirus-mediated oncolysis via upregulation of PUMA and BAX.
Oncogene37(46)6069-6082(2018 11)
Kaverina NV,Kadagidze ZG,Borovjagin AV,Karseladze AI,Kim CK,Lesniak MS,Miska J,Zhang P,Baryshnikova MA,Xiao T,Ornelles D,Cobbs C,Khramtsov A,Ulasov IV
Ipatasertib, a novel Akt inhibitor, induces transcription factor FoxO3a and NF-κB directly regulates PUMA-dependent apoptosis.
Cell death & disease9(9)911(2018 09)
Sun L,Huang Y,Liu Y,Zhao Y,He X,Zhang L,Wang F,Zhang Y
Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer.
Oncogene37(33)4599-4610(2018 08)
Knickelbein K,Tong J,Chen D,Wang YJ,Misale S,Bardelli A,Yu J,Zhang L
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