GFPT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GFPT1
Entrez Gene ID	2673
Full Name	glutamine--fructose-6-phosphate transaminase 1
Synonyms	CMS12,CMSTA1,GFA,GFAT,GFAT 1,GFAT1,GFAT1m,GFPT,GFPT1L,MSLG
General protein information	Preferred Names glutamine--fructose-6-phosphate transaminase 1 Names glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 D-fructose-6-phosphate amidotransferase 1 glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 glutamine:fructose-6-phosphate amidotransferase 1 hexosephosphate aminotransferase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.3
Summary	This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008].
Disorder MIM:	138292
Disorder Html:	Myasthenia, congenital, 12, with tubular aggregates, 610542 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003801.1	XP_016859290.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X1
NM_002056.3	NP_002047.2	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 2
NM_002056.4	NP_002047.2	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 2
NM_001244710.1	NP_001231639.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 1
XM_017003802.1	XP_016859291.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X2
NM_001244710.2	NP_001231639.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 1
XM_017003802.2	XP_016859291.1	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gfpt1	NP_038556.1
Homo sapiens (human)	GFPT1	NP_001231639.1
Macaca mulatta (Rhesus monkey)	GFPT1	XP_001096250.2
Pan troglodytes (chimpanzee)	GFPT1	XP_003949855.1
Xenopus tropicalis (tropical clawed frog)	gfpt1	XP_002935349.1
Canis lupus familiaris (dog)	GFPT1	XP_005626234.1
Gallus gallus (chicken)	GFPT1	NP_001026053.1
Danio rerio (zebrafish)	gfpt1	NP_001030153.1
Bos taurus (cattle)	GFPT1	NP_001103431.1
Rattus norvegicus (Norway rat)	Gfpt1	NP_001005879.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian Target of Rapamycin 2 (MTOR2) and C-MYC Modulate Glucosamine-6-Phosphate Synthesis in Glioblastoma (GBM) Cells Through Glutamine: Fructose-6-Phosphate Aminotransferase 1 (GFAT1).
Liu B, Huang ZB, Chen X, See YX, Chen ZK, Yao HK
Cellular and molecular neurobiology39(3)415-434(2019 Apr)

Leukoencephalopathy due to variants in
Helman G, Sharma S, Crawford J, Patra B, Jain P, Bent SJ, Urtizberea JA, Saran RK, Taft RJ, van der Knaap MS, Simons C
Neurology92(6)e587-e593(2019 02)

IL-8-induced O-GlcNAc modification via GLUT3 and GFAT regulates cancer stem cell-like properties in colon and lung cancer cells.
Shimizu M, Tanaka N
Oncogene38(9)1520-1533(2019 02)

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.
Issop Y, Hathazi D, Khan MM, Rudolf R, Weis J, Spendiff S, Slater CR, Roos A, Lochmüller H
Human molecular genetics27(18)3218-3232(2018 09)

Mutations in GFPT1-related congenital myasthenic syndromes are associated with synaptic morphological defects and underlie a tubular aggregate myopathy with synaptopathy.
Bauché S, Vellieux G, Sternberg D, Fontenille MJ, De Bruyckere E, Davoine CS, Brochier G, Messéant J, Wolf L, Fardeau M, Lacène E, Romero N, Koenig J, Fournier E, Hantaï D, Streichenberger N, Manel V, Lacour A, Nadaj-Pakleza A, Sukno S, Bouhour F, Laforêt P, Fontaine B, Strochlic L, Eymard B, Chevessier F, Stojkovic T, Nicole S
Journal of neurology264(8)1791-1803(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GFPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GFPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu89266	XM_017003801.1 Latest version!	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu89267	XM_017003802.2 Latest version!	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu22604	NM_002056.4 Latest version!	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$335.30 $479.00
OHu23121	NM_001244710.2 Latest version!	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
View Old Accession Versions >>
OHu22604	NM_002056.3	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$335.30 $479.00
OHu23121	NM_001244710.1	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
OHu89267	XM_017003802.1	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GFPT1 ( XM_017003801.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859290.1 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89266
Clone ID Related Accession (Same CDS sequence)	XM_017003801.1
Accession Version	XM_017003801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG CTAGGACTCA GATTGGATCA 
AAATTCACAC GGTGGGGATC ACAGGGAGAA AGAGGCAAAG ACAAGAAAGG AAGCTGCAAT 
CTCTCTCGTG TGGACAGCAC AACCTGCCTT TTCCCGGTGG AAGAAAAAGC AGTGGAGTAT 
TACTTTGCTT CTGATGCAAG TGCTGTCATA GAACACACCA ATCGCGTCAT CTTTCTGGAA 
GATGATGATG TTGCAGCAGT AGTGGATGGA CGTCTTTCTA TCCATCGAAT TAAACGAACT 
GCAGGAGATC ACCCCGGACG AGCTGTGCAA ACACTCCAGA TGGAACTCCA GCAGATCATG 
AAGGGCAACT TCAGTTCATT TATGCAGAAG GAAATATTTG AGCAGCCAGA GTCTGTCGTG 
AACACAATGA GAGGAAGAGT CAACTTTGAT GACTATACTG TGAATTTGGG TGGTTTGAAG 
GATCACATAA AGGAGATCCA GAGATGCCGG CGTTTGATTC TTATTGCTTG TGGAACAAGT 
TACCATGCTG GTGTAGCAAC ACGTCAAGTT CTTGAGGAGC TGACTGAGTT GCCTGTGATG 
GTGGAACTAG CAAGTGACTT CCTGGACAGA AACACACCAG TCTTTCGAGA TGATGTTTGC 
TTTTTCCTTA GTCAATCAGG TGAGACAGCA GATACTTTGA TGGGTCTTCG TTACTGTAAG 
GAGAGAGGAG CTTTAACTGT GGGGATCACA AACACAGTTG GCAGTTCCAT ATCACGGGAG 
ACAGATTGTG GAGTTCATAT TAATGCTGGT CCTGAGATTG GTGTGGCCAG TACAAAGGCT 
TATACCAGCC AGTTTGTATC CCTTGTGATG TTTGCCCTTA TGATGTGTGA TGATCGGATC 
TCCATGCAAG AAAGACGCAA AGAGATCATG CTTGGATTGA AACGGCTGCC TGATTTGATT 
AAGGAAGTAC TGAGCATGGA TGACGAAATT CAGAAACTAG CAACAGAACT TTATCATCAG 
AAGTCAGTTC TGATAATGGG ACGAGGCTAT CATTATGCTA CTTGTCTTGA AGGGGCACTG 
AAAATCAAAG AAATTACTTA TATGCACTCT GAAGGCATCC TTGCTGGTGA ATTGAAACAT 
GGCCCTCTGG CTTTGGTGGA TAAATTGATG CCTGTGATCA TGATCATCAT GAGAGATCAC 
ACTTATGCCA AGTGTCAGAA TGCTCTTCAG CAAGTGGTTG CTCGGCAGGG GCGGCCTGTG 
GTAATTTGTG ATAAGGAGGA TACTGAGACC ATTAAGAACA CAAAAAGAAC GATCAAGGTG 
CCCCACTCAG TGGACTGCTT GCAGGGCATT CTCAGCGTGA TCCCTTTACA GTTGCTGGCT 
TTCCACCTTG CTGTGCTGAG AGGCTATGAT GTTGATTTCC CACGGAATCT TGCCAAATCT 
GTGACTGTAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859290.1
CDS	73..2247
Translation	MTKIRELPSRNCCLIRGKKMNAHATASGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTARTQIGSKFTRWGSQGERGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	XM_017003801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003801.1

ORF Insert Sequence:

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG CTAGGACTCA GATTGGATCA 
AAATTCACAC GGTGGGGATC ACAGGGAGAA AGAGGCAAAG ACAAGAAAGG AAGCTGCAAT 
CTCTCTCGTG TGGACAGCAC AACCTGCCTT TTCCCGGTGG AAGAAAAAGC AGTGGAGTAT 
TACTTTGCTT CTGATGCAAG TGCTGTCATA GAACACACCA ATCGCGTCAT CTTTCTGGAA 
GATGATGATG TTGCAGCAGT AGTGGATGGA CGTCTTTCTA TCCATCGAAT TAAACGAACT 
GCAGGAGATC ACCCCGGACG AGCTGTGCAA ACACTCCAGA TGGAACTCCA GCAGATCATG 
AAGGGCAACT TCAGTTCATT TATGCAGAAG GAAATATTTG AGCAGCCAGA GTCTGTCGTG 
AACACAATGA GAGGAAGAGT CAACTTTGAT GACTATACTG TGAATTTGGG TGGTTTGAAG 
GATCACATAA AGGAGATCCA GAGATGCCGG CGTTTGATTC TTATTGCTTG TGGAACAAGT 
TACCATGCTG GTGTAGCAAC ACGTCAAGTT CTTGAGGAGC TGACTGAGTT GCCTGTGATG 
GTGGAACTAG CAAGTGACTT CCTGGACAGA AACACACCAG TCTTTCGAGA TGATGTTTGC 
TTTTTCCTTA GTCAATCAGG TGAGACAGCA GATACTTTGA TGGGTCTTCG TTACTGTAAG 
GAGAGAGGAG CTTTAACTGT GGGGATCACA AACACAGTTG GCAGTTCCAT ATCACGGGAG 
ACAGATTGTG GAGTTCATAT TAATGCTGGT CCTGAGATTG GTGTGGCCAG TACAAAGGCT 
TATACCAGCC AGTTTGTATC CCTTGTGATG TTTGCCCTTA TGATGTGTGA TGATCGGATC 
TCCATGCAAG AAAGACGCAA AGAGATCATG CTTGGATTGA AACGGCTGCC TGATTTGATT 
AAGGAAGTAC TGAGCATGGA TGACGAAATT CAGAAACTAG CAACAGAACT TTATCATCAG 
AAGTCAGTTC TGATAATGGG ACGAGGCTAT CATTATGCTA CTTGTCTTGA AGGGGCACTG 
AAAATCAAAG AAATTACTTA TATGCACTCT GAAGGCATCC TTGCTGGTGA ATTGAAACAT 
GGCCCTCTGG CTTTGGTGGA TAAATTGATG CCTGTGATCA TGATCATCAT GAGAGATCAC 
ACTTATGCCA AGTGTCAGAA TGCTCTTCAG CAAGTGGTTG CTCGGCAGGG GCGGCCTGTG 
GTAATTTGTG ATAAGGAGGA TACTGAGACC ATTAAGAACA CAAAAAGAAC GATCAAGGTG 
CCCCACTCAG TGGACTGCTT GCAGGGCATT CTCAGCGTGA TCCCTTTACA GTTGCTGGCT 
TTCCACCTTG CTGTGCTGAG AGGCTATGAT GTTGATTTCC CACGGAATCT TGCCAAATCT 
GTGACTGTAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( XM_017003802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859291.1 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu89267
Clone ID Related Accession (Same CDS sequence)	XM_017003802.1 , XM_017003802.2
Accession Version	XM_017003802.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG GCAAAGACAA GAAAGGAAGC 
TGCAATCTCT CTCGTGTGGA CAGCACAACC TGCCTTTTCC CGGTGGAAGA AAAAGCAGTG 
GAGTATTACT TTGCTTCTGA TGCAAGTGCT GTCATAGAAC ACACCAATCG CGTCATCTTT 
CTGGAAGATG ATGATGTTGC AGCAGTAGTG GATGGACGTC TTTCTATCCA TCGAATTAAA 
CGAACTGCAG GAGATCACCC CGGACGAGCT GTGCAAACAC TCCAGATGGA ACTCCAGCAG 
ATCATGAAGG GCAACTTCAG TTCATTTATG CAGAAGGAAA TATTTGAGCA GCCAGAGTCT 
GTCGTGAACA CAATGAGAGG AAGAGTCAAC TTTGATGACT ATACTGTGAA TTTGGGTGGT 
TTGAAGGATC ACATAAAGGA GATCCAGAGA TGCCGGCGTT TGATTCTTAT TGCTTGTGGA 
ACAAGTTACC ATGCTGGTGT AGCAACACGT CAAGTTCTTG AGGAGCTGAC TGAGTTGCCT 
GTGATGGTGG AACTAGCAAG TGACTTCCTG GACAGAAACA CACCAGTCTT TCGAGATGAT 
GTTTGCTTTT TCCTTAGTCA ATCAGGTGAG ACAGCAGATA CTTTGATGGG TCTTCGTTAC 
TGTAAGGAGA GAGGAGCTTT AACTGTGGGG ATCACAAACA CAGTTGGCAG TTCCATATCA 
CGGGAGACAG ATTGTGGAGT TCATATTAAT GCTGGTCCTG AGATTGGTGT GGCCAGTACA 
AAGGCTTATA CCAGCCAGTT TGTATCCCTT GTGATGTTTG CCCTTATGAT GTGTGATGAT 
CGGATCTCCA TGCAAGAAAG ACGCAAAGAG ATCATGCTTG GATTGAAACG GCTGCCTGAT 
TTGATTAAGG AAGTACTGAG CATGGATGAC GAAATTCAGA AACTAGCAAC AGAACTTTAT 
CATCAGAAGT CAGTTCTGAT AATGGGACGA GGCTATCATT ATGCTACTTG TCTTGAAGGG 
GCACTGAAAA TCAAAGAAAT TACTTATATG CACTCTGAAG GCATCCTTGC TGGTGAATTG 
AAACATGGCC CTCTGGCTTT GGTGGATAAA TTGATGCCTG TGATCATGAT CATCATGAGA 
GATCACACTT ATGCCAAGTG TCAGAATGCT CTTCAGCAAG TGGTTGCTCG GCAGGGGCGG 
CCTGTGGTAA TTTGTGATAA GGAGGATACT GAGACCATTA AGAACACAAA AAGAACGATC 
AAGGTGCCCC ACTCAGTGGA CTGCTTGCAG GGCATTCTCA GCGTGATCCC TTTACAGTTG 
CTGGCTTTCC ACCTTGCTGT GCTGAGAGGC TATGATGTTG ATTTCCCACG GAATCTTGCC 
AAATCTGTGA CTGTAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859291.1
CDS	65..2185
Translation	MTKIRELPSRNCCLIRGKKMNAHATASGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	XM_017003802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003802.1

ORF Insert Sequence:

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG GCAAAGACAA GAAAGGAAGC 
TGCAATCTCT CTCGTGTGGA CAGCACAACC TGCCTTTTCC CGGTGGAAGA AAAAGCAGTG 
GAGTATTACT TTGCTTCTGA TGCAAGTGCT GTCATAGAAC ACACCAATCG CGTCATCTTT 
CTGGAAGATG ATGATGTTGC AGCAGTAGTG GATGGACGTC TTTCTATCCA TCGAATTAAA 
CGAACTGCAG GAGATCACCC CGGACGAGCT GTGCAAACAC TCCAGATGGA ACTCCAGCAG 
ATCATGAAGG GCAACTTCAG TTCATTTATG CAGAAGGAAA TATTTGAGCA GCCAGAGTCT 
GTCGTGAACA CAATGAGAGG AAGAGTCAAC TTTGATGACT ATACTGTGAA TTTGGGTGGT 
TTGAAGGATC ACATAAAGGA GATCCAGAGA TGCCGGCGTT TGATTCTTAT TGCTTGTGGA 
ACAAGTTACC ATGCTGGTGT AGCAACACGT CAAGTTCTTG AGGAGCTGAC TGAGTTGCCT 
GTGATGGTGG AACTAGCAAG TGACTTCCTG GACAGAAACA CACCAGTCTT TCGAGATGAT 
GTTTGCTTTT TCCTTAGTCA ATCAGGTGAG ACAGCAGATA CTTTGATGGG TCTTCGTTAC 
TGTAAGGAGA GAGGAGCTTT AACTGTGGGG ATCACAAACA CAGTTGGCAG TTCCATATCA 
CGGGAGACAG ATTGTGGAGT TCATATTAAT GCTGGTCCTG AGATTGGTGT GGCCAGTACA 
AAGGCTTATA CCAGCCAGTT TGTATCCCTT GTGATGTTTG CCCTTATGAT GTGTGATGAT 
CGGATCTCCA TGCAAGAAAG ACGCAAAGAG ATCATGCTTG GATTGAAACG GCTGCCTGAT 
TTGATTAAGG AAGTACTGAG CATGGATGAC GAAATTCAGA AACTAGCAAC AGAACTTTAT 
CATCAGAAGT CAGTTCTGAT AATGGGACGA GGCTATCATT ATGCTACTTG TCTTGAAGGG 
GCACTGAAAA TCAAAGAAAT TACTTATATG CACTCTGAAG GCATCCTTGC TGGTGAATTG 
AAACATGGCC CTCTGGCTTT GGTGGATAAA TTGATGCCTG TGATCATGAT CATCATGAGA 
GATCACACTT ATGCCAAGTG TCAGAATGCT CTTCAGCAAG TGGTTGCTCG GCAGGGGCGG 
CCTGTGGTAA TTTGTGATAA GGAGGATACT GAGACCATTA AGAACACAAA AAGAACGATC 
AAGGTGCCCC ACTCAGTGGA CTGCTTGCAG GGCATTCTCA GCGTGATCCC TTTACAGTTG 
CTGGCTTTCC ACCTTGCTGT GCTGAGAGGC TATGATGTTG ATTTCCCACG GAATCTTGCC 
AAATCTGTGA CTGTAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( XM_017003802.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859291.1 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.

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CloneID	OHu89267
Clone ID Related Accession (Same CDS sequence)	XM_017003802.1 , XM_017003802.2
Accession Version	XM_017003802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG GCAAAGACAA GAAAGGAAGC 
TGCAATCTCT CTCGTGTGGA CAGCACAACC TGCCTTTTCC CGGTGGAAGA AAAAGCAGTG 
GAGTATTACT TTGCTTCTGA TGCAAGTGCT GTCATAGAAC ACACCAATCG CGTCATCTTT 
CTGGAAGATG ATGATGTTGC AGCAGTAGTG GATGGACGTC TTTCTATCCA TCGAATTAAA 
CGAACTGCAG GAGATCACCC CGGACGAGCT GTGCAAACAC TCCAGATGGA ACTCCAGCAG 
ATCATGAAGG GCAACTTCAG TTCATTTATG CAGAAGGAAA TATTTGAGCA GCCAGAGTCT 
GTCGTGAACA CAATGAGAGG AAGAGTCAAC TTTGATGACT ATACTGTGAA TTTGGGTGGT 
TTGAAGGATC ACATAAAGGA GATCCAGAGA TGCCGGCGTT TGATTCTTAT TGCTTGTGGA 
ACAAGTTACC ATGCTGGTGT AGCAACACGT CAAGTTCTTG AGGAGCTGAC TGAGTTGCCT 
GTGATGGTGG AACTAGCAAG TGACTTCCTG GACAGAAACA CACCAGTCTT TCGAGATGAT 
GTTTGCTTTT TCCTTAGTCA ATCAGGTGAG ACAGCAGATA CTTTGATGGG TCTTCGTTAC 
TGTAAGGAGA GAGGAGCTTT AACTGTGGGG ATCACAAACA CAGTTGGCAG TTCCATATCA 
CGGGAGACAG ATTGTGGAGT TCATATTAAT GCTGGTCCTG AGATTGGTGT GGCCAGTACA 
AAGGCTTATA CCAGCCAGTT TGTATCCCTT GTGATGTTTG CCCTTATGAT GTGTGATGAT 
CGGATCTCCA TGCAAGAAAG ACGCAAAGAG ATCATGCTTG GATTGAAACG GCTGCCTGAT 
TTGATTAAGG AAGTACTGAG CATGGATGAC GAAATTCAGA AACTAGCAAC AGAACTTTAT 
CATCAGAAGT CAGTTCTGAT AATGGGACGA GGCTATCATT ATGCTACTTG TCTTGAAGGG 
GCACTGAAAA TCAAAGAAAT TACTTATATG CACTCTGAAG GCATCCTTGC TGGTGAATTG 
AAACATGGCC CTCTGGCTTT GGTGGATAAA TTGATGCCTG TGATCATGAT CATCATGAGA 
GATCACACTT ATGCCAAGTG TCAGAATGCT CTTCAGCAAG TGGTTGCTCG GCAGGGGCGG 
CCTGTGGTAA TTTGTGATAA GGAGGATACT GAGACCATTA AGAACACAAA AAGAACGATC 
AAGGTGCCCC ACTCAGTGGA CTGCTTGCAG GGCATTCTCA GCGTGATCCC TTTACAGTTG 
CTGGCTTTCC ACCTTGCTGT GCTGAGAGGC TATGATGTTG ATTTCCCACG GAATCTTGCC 
AAATCTGTGA CTGTAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859291.1
CDS	65..2185
Translation	MTKIRELPSRNCCLIRGKKMNAHATASGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	XM_017003802.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003802.2

ORF Insert Sequence:

ATGACTAAAA TAAGAGAGTT GCCTTCAAGA AATTGCTGCC TGATCAGAGG AAAGAAAATG 
AATGCACATG CAACAGCTTC AGGTATATTT GCTTACTTAA ACTACCATGT TCCTCGAACG 
AGACGAGAAA TCCTGGAGAC CCTAATCAAA GGCCTTCAGA GACTGGAGTA CAGAGGATAT 
GATTCTGCTG GTGTGGGATT TGATGGAGGC AATGATAAAG ATTGGGAAGC CAATGCCTGC 
AAAATCCAGC TTATTAAGAA GAAAGGAAAA GTTAAGGCAC TGGATGAAGA AGTTCACAAG 
CAACAAGATA TGGATTTGGA TATAGAATTT GATGTACACC TTGGAATAGC TCATACCCGT 
TGGGCAACAC ATGGAGAACC CAGTCCTGTC AATAGCCACC CCCAGCGCTC TGATAAAAAT 
AATGAATTTA TCGTTATTCA CAATGGAATC ATCACCAACT ACAAAGACTT GAAAAAGTTT 
TTGGAAAGCA AAGGCTATGA CTTCGAATCT GAAACAGACA CAGAGACAAT TGCCAAGCTC 
GTTAAGTATA TGTATGACAA TCGGGAAAGT CAAGATACCA GCTTTACTAC CTTGGTGGAG 
AGAGTTATCC AACAATTGGA AGGTGCTTTT GCACTTGTGT TTAAAAGTGT TCATTTTCCC 
GGGCAAGCAG TTGGCACAAG GCGAGGTAGC CCTCTGTTGA TTGGTGTACG GAGTGAACAT 
AAACTTTCTA CTGATCACAT TCCTATACTC TACAGAACAG GCAAAGACAA GAAAGGAAGC 
TGCAATCTCT CTCGTGTGGA CAGCACAACC TGCCTTTTCC CGGTGGAAGA AAAAGCAGTG 
GAGTATTACT TTGCTTCTGA TGCAAGTGCT GTCATAGAAC ACACCAATCG CGTCATCTTT 
CTGGAAGATG ATGATGTTGC AGCAGTAGTG GATGGACGTC TTTCTATCCA TCGAATTAAA 
CGAACTGCAG GAGATCACCC CGGACGAGCT GTGCAAACAC TCCAGATGGA ACTCCAGCAG 
ATCATGAAGG GCAACTTCAG TTCATTTATG CAGAAGGAAA TATTTGAGCA GCCAGAGTCT 
GTCGTGAACA CAATGAGAGG AAGAGTCAAC TTTGATGACT ATACTGTGAA TTTGGGTGGT 
TTGAAGGATC ACATAAAGGA GATCCAGAGA TGCCGGCGTT TGATTCTTAT TGCTTGTGGA 
ACAAGTTACC ATGCTGGTGT AGCAACACGT CAAGTTCTTG AGGAGCTGAC TGAGTTGCCT 
GTGATGGTGG AACTAGCAAG TGACTTCCTG GACAGAAACA CACCAGTCTT TCGAGATGAT 
GTTTGCTTTT TCCTTAGTCA ATCAGGTGAG ACAGCAGATA CTTTGATGGG TCTTCGTTAC 
TGTAAGGAGA GAGGAGCTTT AACTGTGGGG ATCACAAACA CAGTTGGCAG TTCCATATCA 
CGGGAGACAG ATTGTGGAGT TCATATTAAT GCTGGTCCTG AGATTGGTGT GGCCAGTACA 
AAGGCTTATA CCAGCCAGTT TGTATCCCTT GTGATGTTTG CCCTTATGAT GTGTGATGAT 
CGGATCTCCA TGCAAGAAAG ACGCAAAGAG ATCATGCTTG GATTGAAACG GCTGCCTGAT 
TTGATTAAGG AAGTACTGAG CATGGATGAC GAAATTCAGA AACTAGCAAC AGAACTTTAT 
CATCAGAAGT CAGTTCTGAT AATGGGACGA GGCTATCATT ATGCTACTTG TCTTGAAGGG 
GCACTGAAAA TCAAAGAAAT TACTTATATG CACTCTGAAG GCATCCTTGC TGGTGAATTG 
AAACATGGCC CTCTGGCTTT GGTGGATAAA TTGATGCCTG TGATCATGAT CATCATGAGA 
GATCACACTT ATGCCAAGTG TCAGAATGCT CTTCAGCAAG TGGTTGCTCG GCAGGGGCGG 
CCTGTGGTAA TTTGTGATAA GGAGGATACT GAGACCATTA AGAACACAAA AAGAACGATC 
AAGGTGCCCC ACTCAGTGGA CTGCTTGCAG GGCATTCTCA GCGTGATCCC TTTACAGTTG 
CTGGCTTTCC ACCTTGCTGT GCTGAGAGGC TATGATGTTG ATTTCCCACG GAATCTTGCC 
AAATCTGTGA CTGTAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( NM_002056.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002047.2 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.

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CloneID	OHu22604
Clone ID Related Accession (Same CDS sequence)	NM_002056.3 , NM_002056.4
Accession Version	NM_002056.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551542400000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560595.1, M90516.1 and AC114772.5. On Sep 24, 2011 this sequence version replaced NM_002056.2. Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.128952.1, SRR1660805.73734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCAA TCTCTCTCGT 
GTGGACAGCA CAACCTGCCT TTTCCCGGTG GAAGAAAAAG CAGTGGAGTA TTACTTTGCT 
TCTGATGCAA GTGCTGTCAT AGAACACACC AATCGCGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TAGTGGATGG ACGTCTTTCT ATCCATCGAA TTAAACGAAC TGCAGGAGAT 
CACCCCGGAC GAGCTGTGCA AACACTCCAG ATGGAACTCC AGCAGATCAT GAAGGGCAAC 
TTCAGTTCAT TTATGCAGAA GGAAATATTT GAGCAGCCAG AGTCTGTCGT GAACACAATG 
AGAGGAAGAG TCAACTTTGA TGACTATACT GTGAATTTGG GTGGTTTGAA GGATCACATA 
AAGGAGATCC AGAGATGCCG GCGTTTGATT CTTATTGCTT GTGGAACAAG TTACCATGCT 
GGTGTAGCAA CACGTCAAGT TCTTGAGGAG CTGACTGAGT TGCCTGTGAT GGTGGAACTA 
GCAAGTGACT TCCTGGACAG AAACACACCA GTCTTTCGAG ATGATGTTTG CTTTTTCCTT 
AGTCAATCAG GTGAGACAGC AGATACTTTG ATGGGTCTTC GTTACTGTAA GGAGAGAGGA 
GCTTTAACTG TGGGGATCAC AAACACAGTT GGCAGTTCCA TATCACGGGA GACAGATTGT 
GGAGTTCATA TTAATGCTGG TCCTGAGATT GGTGTGGCCA GTACAAAGGC TTATACCAGC 
CAGTTTGTAT CCCTTGTGAT GTTTGCCCTT ATGATGTGTG ATGATCGGAT CTCCATGCAA 
GAAAGACGCA AAGAGATCAT GCTTGGATTG AAACGGCTGC CTGATTTGAT TAAGGAAGTA 
CTGAGCATGG ATGACGAAAT TCAGAAACTA GCAACAGAAC TTTATCATCA GAAGTCAGTT 
CTGATAATGG GACGAGGCTA TCATTATGCT ACTTGTCTTG AAGGGGCACT GAAAATCAAA 
GAAATTACTT ATATGCACTC TGAAGGCATC CTTGCTGGTG AATTGAAACA TGGCCCTCTG 
GCTTTGGTGG ATAAATTGAT GCCTGTGATC ATGATCATCA TGAGAGATCA CACTTATGCC 
AAGTGTCAGA ATGCTCTTCA GCAAGTGGTT GCTCGGCAGG GGCGGCCTGT GGTAATTTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAC ACAAAAAGAA CGATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGCGTG ATCCCTTTAC AGTTGCTGGC TTTCCACCTT 
GCTGTGCTGA GAGGCTATGA TGTTGATTTC CCACGGAATC TTGCCAAATC TGTGACTGTA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002047.2
CDS	184..2229
Translation	MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_002056.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002056.3

ORF Insert Sequence:

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCAA TCTCTCTCGT 
GTGGACAGCA CAACCTGCCT TTTCCCGGTG GAAGAAAAAG CAGTGGAGTA TTACTTTGCT 
TCTGATGCAA GTGCTGTCAT AGAACACACC AATCGCGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TAGTGGATGG ACGTCTTTCT ATCCATCGAA TTAAACGAAC TGCAGGAGAT 
CACCCCGGAC GAGCTGTGCA AACACTCCAG ATGGAACTCC AGCAGATCAT GAAGGGCAAC 
TTCAGTTCAT TTATGCAGAA GGAAATATTT GAGCAGCCAG AGTCTGTCGT GAACACAATG 
AGAGGAAGAG TCAACTTTGA TGACTATACT GTGAATTTGG GTGGTTTGAA GGATCACATA 
AAGGAGATCC AGAGATGCCG GCGTTTGATT CTTATTGCTT GTGGAACAAG TTACCATGCT 
GGTGTAGCAA CACGTCAAGT TCTTGAGGAG CTGACTGAGT TGCCTGTGAT GGTGGAACTA 
GCAAGTGACT TCCTGGACAG AAACACACCA GTCTTTCGAG ATGATGTTTG CTTTTTCCTT 
AGTCAATCAG GTGAGACAGC AGATACTTTG ATGGGTCTTC GTTACTGTAA GGAGAGAGGA 
GCTTTAACTG TGGGGATCAC AAACACAGTT GGCAGTTCCA TATCACGGGA GACAGATTGT 
GGAGTTCATA TTAATGCTGG TCCTGAGATT GGTGTGGCCA GTACAAAGGC TTATACCAGC 
CAGTTTGTAT CCCTTGTGAT GTTTGCCCTT ATGATGTGTG ATGATCGGAT CTCCATGCAA 
GAAAGACGCA AAGAGATCAT GCTTGGATTG AAACGGCTGC CTGATTTGAT TAAGGAAGTA 
CTGAGCATGG ATGACGAAAT TCAGAAACTA GCAACAGAAC TTTATCATCA GAAGTCAGTT 
CTGATAATGG GACGAGGCTA TCATTATGCT ACTTGTCTTG AAGGGGCACT GAAAATCAAA 
GAAATTACTT ATATGCACTC TGAAGGCATC CTTGCTGGTG AATTGAAACA TGGCCCTCTG 
GCTTTGGTGG ATAAATTGAT GCCTGTGATC ATGATCATCA TGAGAGATCA CACTTATGCC 
AAGTGTCAGA ATGCTCTTCA GCAAGTGGTT GCTCGGCAGG GGCGGCCTGT GGTAATTTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAC ACAAAAAGAA CGATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGCGTG ATCCCTTTAC AGTTGCTGGC TTTCCACCTT 
GCTGTGCTGA GAGGCTATGA TGTTGATTTC CCACGGAATC TTGCCAAATC TGTGACTGTA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( NM_002056.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002047.2 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22604
Clone ID Related Accession (Same CDS sequence)	NM_002056.3 , NM_002056.4
Accession Version	NM_002056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560595.1, M90516.1 and AC114772.5. On Jun 2, 2019 this sequence version replaced NM_002056.3. Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.128952.1, SRR1660805.73734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCAA TCTCTCTCGT 
GTGGACAGCA CAACCTGCCT TTTCCCGGTG GAAGAAAAAG CAGTGGAGTA TTACTTTGCT 
TCTGATGCAA GTGCTGTCAT AGAACACACC AATCGCGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TAGTGGATGG ACGTCTTTCT ATCCATCGAA TTAAACGAAC TGCAGGAGAT 
CACCCCGGAC GAGCTGTGCA AACACTCCAG ATGGAACTCC AGCAGATCAT GAAGGGCAAC 
TTCAGTTCAT TTATGCAGAA GGAAATATTT GAGCAGCCAG AGTCTGTCGT GAACACAATG 
AGAGGAAGAG TCAACTTTGA TGACTATACT GTGAATTTGG GTGGTTTGAA GGATCACATA 
AAGGAGATCC AGAGATGCCG GCGTTTGATT CTTATTGCTT GTGGAACAAG TTACCATGCT 
GGTGTAGCAA CACGTCAAGT TCTTGAGGAG CTGACTGAGT TGCCTGTGAT GGTGGAACTA 
GCAAGTGACT TCCTGGACAG AAACACACCA GTCTTTCGAG ATGATGTTTG CTTTTTCCTT 
AGTCAATCAG GTGAGACAGC AGATACTTTG ATGGGTCTTC GTTACTGTAA GGAGAGAGGA 
GCTTTAACTG TGGGGATCAC AAACACAGTT GGCAGTTCCA TATCACGGGA GACAGATTGT 
GGAGTTCATA TTAATGCTGG TCCTGAGATT GGTGTGGCCA GTACAAAGGC TTATACCAGC 
CAGTTTGTAT CCCTTGTGAT GTTTGCCCTT ATGATGTGTG ATGATCGGAT CTCCATGCAA 
GAAAGACGCA AAGAGATCAT GCTTGGATTG AAACGGCTGC CTGATTTGAT TAAGGAAGTA 
CTGAGCATGG ATGACGAAAT TCAGAAACTA GCAACAGAAC TTTATCATCA GAAGTCAGTT 
CTGATAATGG GACGAGGCTA TCATTATGCT ACTTGTCTTG AAGGGGCACT GAAAATCAAA 
GAAATTACTT ATATGCACTC TGAAGGCATC CTTGCTGGTG AATTGAAACA TGGCCCTCTG 
GCTTTGGTGG ATAAATTGAT GCCTGTGATC ATGATCATCA TGAGAGATCA CACTTATGCC 
AAGTGTCAGA ATGCTCTTCA GCAAGTGGTT GCTCGGCAGG GGCGGCCTGT GGTAATTTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAC ACAAAAAGAA CGATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGCGTG ATCCCTTTAC AGTTGCTGGC TTTCCACCTT 
GCTGTGCTGA GAGGCTATGA TGTTGATTTC CCACGGAATC TTGCCAAATC TGTGACTGTA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002047.2
CDS	157..2202
Translation	MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_002056.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002056.4

ORF Insert Sequence:

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGGCAAA GACAAGAAAG GAAGCTGCAA TCTCTCTCGT 
GTGGACAGCA CAACCTGCCT TTTCCCGGTG GAAGAAAAAG CAGTGGAGTA TTACTTTGCT 
TCTGATGCAA GTGCTGTCAT AGAACACACC AATCGCGTCA TCTTTCTGGA AGATGATGAT 
GTTGCAGCAG TAGTGGATGG ACGTCTTTCT ATCCATCGAA TTAAACGAAC TGCAGGAGAT 
CACCCCGGAC GAGCTGTGCA AACACTCCAG ATGGAACTCC AGCAGATCAT GAAGGGCAAC 
TTCAGTTCAT TTATGCAGAA GGAAATATTT GAGCAGCCAG AGTCTGTCGT GAACACAATG 
AGAGGAAGAG TCAACTTTGA TGACTATACT GTGAATTTGG GTGGTTTGAA GGATCACATA 
AAGGAGATCC AGAGATGCCG GCGTTTGATT CTTATTGCTT GTGGAACAAG TTACCATGCT 
GGTGTAGCAA CACGTCAAGT TCTTGAGGAG CTGACTGAGT TGCCTGTGAT GGTGGAACTA 
GCAAGTGACT TCCTGGACAG AAACACACCA GTCTTTCGAG ATGATGTTTG CTTTTTCCTT 
AGTCAATCAG GTGAGACAGC AGATACTTTG ATGGGTCTTC GTTACTGTAA GGAGAGAGGA 
GCTTTAACTG TGGGGATCAC AAACACAGTT GGCAGTTCCA TATCACGGGA GACAGATTGT 
GGAGTTCATA TTAATGCTGG TCCTGAGATT GGTGTGGCCA GTACAAAGGC TTATACCAGC 
CAGTTTGTAT CCCTTGTGAT GTTTGCCCTT ATGATGTGTG ATGATCGGAT CTCCATGCAA 
GAAAGACGCA AAGAGATCAT GCTTGGATTG AAACGGCTGC CTGATTTGAT TAAGGAAGTA 
CTGAGCATGG ATGACGAAAT TCAGAAACTA GCAACAGAAC TTTATCATCA GAAGTCAGTT 
CTGATAATGG GACGAGGCTA TCATTATGCT ACTTGTCTTG AAGGGGCACT GAAAATCAAA 
GAAATTACTT ATATGCACTC TGAAGGCATC CTTGCTGGTG AATTGAAACA TGGCCCTCTG 
GCTTTGGTGG ATAAATTGAT GCCTGTGATC ATGATCATCA TGAGAGATCA CACTTATGCC 
AAGTGTCAGA ATGCTCTTCA GCAAGTGGTT GCTCGGCAGG GGCGGCCTGT GGTAATTTGT 
GATAAGGAGG ATACTGAGAC CATTAAGAAC ACAAAAAGAA CGATCAAGGT GCCCCACTCA 
GTGGACTGCT TGCAGGGCAT TCTCAGCGTG ATCCCTTTAC AGTTGCTGGC TTTCCACCTT 
GCTGTGCTGA GAGGCTATGA TGTTGATTTC CCACGGAATC TTGCCAAATC TGTGACTGTA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( NM_001244710.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231639.1 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.

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CloneID	OHu23121
Clone ID Related Accession (Same CDS sequence)	NM_001244710.1 , NM_001244710.2
Accession Version	NM_001244710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558108800000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560595.1, DB133199.1, AF334737.1, M90516.1 and AC114772.5. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript for this combination of exons and introns was available for the full length of the gene. The extent of this transcript is supported by mouse accession AF334736.1 and PubMed ID: 11679416. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.245495.1, SRR1803614.32072.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END##

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGCTAGG ACTCAGATTG GATCAAAATT CACACGGTGG 
GGATCACAGG GAGAAAGAGG CAAAGACAAG AAAGGAAGCT GCAATCTCTC TCGTGTGGAC 
AGCACAACCT GCCTTTTCCC GGTGGAAGAA AAAGCAGTGG AGTATTACTT TGCTTCTGAT 
GCAAGTGCTG TCATAGAACA CACCAATCGC GTCATCTTTC TGGAAGATGA TGATGTTGCA 
GCAGTAGTGG ATGGACGTCT TTCTATCCAT CGAATTAAAC GAACTGCAGG AGATCACCCC 
GGACGAGCTG TGCAAACACT CCAGATGGAA CTCCAGCAGA TCATGAAGGG CAACTTCAGT 
TCATTTATGC AGAAGGAAAT ATTTGAGCAG CCAGAGTCTG TCGTGAACAC AATGAGAGGA 
AGAGTCAACT TTGATGACTA TACTGTGAAT TTGGGTGGTT TGAAGGATCA CATAAAGGAG 
ATCCAGAGAT GCCGGCGTTT GATTCTTATT GCTTGTGGAA CAAGTTACCA TGCTGGTGTA 
GCAACACGTC AAGTTCTTGA GGAGCTGACT GAGTTGCCTG TGATGGTGGA ACTAGCAAGT 
GACTTCCTGG ACAGAAACAC ACCAGTCTTT CGAGATGATG TTTGCTTTTT CCTTAGTCAA 
TCAGGTGAGA CAGCAGATAC TTTGATGGGT CTTCGTTACT GTAAGGAGAG AGGAGCTTTA 
ACTGTGGGGA TCACAAACAC AGTTGGCAGT TCCATATCAC GGGAGACAGA TTGTGGAGTT 
CATATTAATG CTGGTCCTGA GATTGGTGTG GCCAGTACAA AGGCTTATAC CAGCCAGTTT 
GTATCCCTTG TGATGTTTGC CCTTATGATG TGTGATGATC GGATCTCCAT GCAAGAAAGA 
CGCAAAGAGA TCATGCTTGG ATTGAAACGG CTGCCTGATT TGATTAAGGA AGTACTGAGC 
ATGGATGACG AAATTCAGAA ACTAGCAACA GAACTTTATC ATCAGAAGTC AGTTCTGATA 
ATGGGACGAG GCTATCATTA TGCTACTTGT CTTGAAGGGG CACTGAAAAT CAAAGAAATT 
ACTTATATGC ACTCTGAAGG CATCCTTGCT GGTGAATTGA AACATGGCCC TCTGGCTTTG 
GTGGATAAAT TGATGCCTGT GATCATGATC ATCATGAGAG ATCACACTTA TGCCAAGTGT 
CAGAATGCTC TTCAGCAAGT GGTTGCTCGG CAGGGGCGGC CTGTGGTAAT TTGTGATAAG 
GAGGATACTG AGACCATTAA GAACACAAAA AGAACGATCA AGGTGCCCCA CTCAGTGGAC 
TGCTTGCAGG GCATTCTCAG CGTGATCCCT TTACAGTTGC TGGCTTTCCA CCTTGCTGTG 
CTGAGAGGCT ATGATGTTGA TTTCCCACGG AATCTTGCCA AATCTGTGAC TGTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231639.1
CDS	184..2283
Translation	MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTARTQIGSKFTRWGSQGERGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_001244710.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244710.1

ORF Insert Sequence:

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGCTAGG ACTCAGATTG GATCAAAATT CACACGGTGG 
GGATCACAGG GAGAAAGAGG CAAAGACAAG AAAGGAAGCT GCAATCTCTC TCGTGTGGAC 
AGCACAACCT GCCTTTTCCC GGTGGAAGAA AAAGCAGTGG AGTATTACTT TGCTTCTGAT 
GCAAGTGCTG TCATAGAACA CACCAATCGC GTCATCTTTC TGGAAGATGA TGATGTTGCA 
GCAGTAGTGG ATGGACGTCT TTCTATCCAT CGAATTAAAC GAACTGCAGG AGATCACCCC 
GGACGAGCTG TGCAAACACT CCAGATGGAA CTCCAGCAGA TCATGAAGGG CAACTTCAGT 
TCATTTATGC AGAAGGAAAT ATTTGAGCAG CCAGAGTCTG TCGTGAACAC AATGAGAGGA 
AGAGTCAACT TTGATGACTA TACTGTGAAT TTGGGTGGTT TGAAGGATCA CATAAAGGAG 
ATCCAGAGAT GCCGGCGTTT GATTCTTATT GCTTGTGGAA CAAGTTACCA TGCTGGTGTA 
GCAACACGTC AAGTTCTTGA GGAGCTGACT GAGTTGCCTG TGATGGTGGA ACTAGCAAGT 
GACTTCCTGG ACAGAAACAC ACCAGTCTTT CGAGATGATG TTTGCTTTTT CCTTAGTCAA 
TCAGGTGAGA CAGCAGATAC TTTGATGGGT CTTCGTTACT GTAAGGAGAG AGGAGCTTTA 
ACTGTGGGGA TCACAAACAC AGTTGGCAGT TCCATATCAC GGGAGACAGA TTGTGGAGTT 
CATATTAATG CTGGTCCTGA GATTGGTGTG GCCAGTACAA AGGCTTATAC CAGCCAGTTT 
GTATCCCTTG TGATGTTTGC CCTTATGATG TGTGATGATC GGATCTCCAT GCAAGAAAGA 
CGCAAAGAGA TCATGCTTGG ATTGAAACGG CTGCCTGATT TGATTAAGGA AGTACTGAGC 
ATGGATGACG AAATTCAGAA ACTAGCAACA GAACTTTATC ATCAGAAGTC AGTTCTGATA 
ATGGGACGAG GCTATCATTA TGCTACTTGT CTTGAAGGGG CACTGAAAAT CAAAGAAATT 
ACTTATATGC ACTCTGAAGG CATCCTTGCT GGTGAATTGA AACATGGCCC TCTGGCTTTG 
GTGGATAAAT TGATGCCTGT GATCATGATC ATCATGAGAG ATCACACTTA TGCCAAGTGT 
CAGAATGCTC TTCAGCAAGT GGTTGCTCGG CAGGGGCGGC CTGTGGTAAT TTGTGATAAG 
GAGGATACTG AGACCATTAA GAACACAAAA AGAACGATCA AGGTGCCCCA CTCAGTGGAC 
TGCTTGCAGG GCATTCTCAG CGTGATCCCT TTACAGTTGC TGGCTTTCCA CCTTGCTGTG 
CTGAGAGGCT ATGATGTTGA TTTCCCACGG AATCTTGCCA AATCTGTGAC TGTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFPT1 ( NM_001244710.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231639.1 Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.

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CloneID	OHu23121
Clone ID Related Accession (Same CDS sequence)	NM_001244710.1 , NM_001244710.2
Accession Version	NM_001244710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Homo sapiens(human)
Product	glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI560595.1, DB133199.1, AF334737.1, M90516.1 and AC114772.5. On Sep 19, 2019 this sequence version replaced NM_001244710.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.245495.1, SRR1803614.32072.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END##

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGCTAGG ACTCAGATTG GATCAAAATT CACACGGTGG 
GGATCACAGG GAGAAAGAGG CAAAGACAAG AAAGGAAGCT GCAATCTCTC TCGTGTGGAC 
AGCACAACCT GCCTTTTCCC GGTGGAAGAA AAAGCAGTGG AGTATTACTT TGCTTCTGAT 
GCAAGTGCTG TCATAGAACA CACCAATCGC GTCATCTTTC TGGAAGATGA TGATGTTGCA 
GCAGTAGTGG ATGGACGTCT TTCTATCCAT CGAATTAAAC GAACTGCAGG AGATCACCCC 
GGACGAGCTG TGCAAACACT CCAGATGGAA CTCCAGCAGA TCATGAAGGG CAACTTCAGT 
TCATTTATGC AGAAGGAAAT ATTTGAGCAG CCAGAGTCTG TCGTGAACAC AATGAGAGGA 
AGAGTCAACT TTGATGACTA TACTGTGAAT TTGGGTGGTT TGAAGGATCA CATAAAGGAG 
ATCCAGAGAT GCCGGCGTTT GATTCTTATT GCTTGTGGAA CAAGTTACCA TGCTGGTGTA 
GCAACACGTC AAGTTCTTGA GGAGCTGACT GAGTTGCCTG TGATGGTGGA ACTAGCAAGT 
GACTTCCTGG ACAGAAACAC ACCAGTCTTT CGAGATGATG TTTGCTTTTT CCTTAGTCAA 
TCAGGTGAGA CAGCAGATAC TTTGATGGGT CTTCGTTACT GTAAGGAGAG AGGAGCTTTA 
ACTGTGGGGA TCACAAACAC AGTTGGCAGT TCCATATCAC GGGAGACAGA TTGTGGAGTT 
CATATTAATG CTGGTCCTGA GATTGGTGTG GCCAGTACAA AGGCTTATAC CAGCCAGTTT 
GTATCCCTTG TGATGTTTGC CCTTATGATG TGTGATGATC GGATCTCCAT GCAAGAAAGA 
CGCAAAGAGA TCATGCTTGG ATTGAAACGG CTGCCTGATT TGATTAAGGA AGTACTGAGC 
ATGGATGACG AAATTCAGAA ACTAGCAACA GAACTTTATC ATCAGAAGTC AGTTCTGATA 
ATGGGACGAG GCTATCATTA TGCTACTTGT CTTGAAGGGG CACTGAAAAT CAAAGAAATT 
ACTTATATGC ACTCTGAAGG CATCCTTGCT GGTGAATTGA AACATGGCCC TCTGGCTTTG 
GTGGATAAAT TGATGCCTGT GATCATGATC ATCATGAGAG ATCACACTTA TGCCAAGTGT 
CAGAATGCTC TTCAGCAAGT GGTTGCTCGG CAGGGGCGGC CTGTGGTAAT TTGTGATAAG 
GAGGATACTG AGACCATTAA GAACACAAAA AGAACGATCA AGGTGCCCCA CTCAGTGGAC 
TGCTTGCAGG GCATTCTCAG CGTGATCCCT TTACAGTTGC TGGCTTTCCA CCTTGCTGTG 
CTGAGAGGCT ATGATGTTGA TTTCCCACGG AATCTTGCCA AATCTGTGAC TGTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231639.1
CDS	157..2256
Translation	MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTARTQIGSKFTRWGSQGERGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Target ORF information:

RefSeq Version	NM_001244710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamine--fructose-6-phosphate transaminase 1 (GFPT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244710.2

ORF Insert Sequence:

ATGTGTGGTA TATTTGCTTA CTTAAACTAC CATGTTCCTC GAACGAGACG AGAAATCCTG 
GAGACCCTAA TCAAAGGCCT TCAGAGACTG GAGTACAGAG GATATGATTC TGCTGGTGTG 
GGATTTGATG GAGGCAATGA TAAAGATTGG GAAGCCAATG CCTGCAAAAT CCAGCTTATT 
AAGAAGAAAG GAAAAGTTAA GGCACTGGAT GAAGAAGTTC ACAAGCAACA AGATATGGAT 
TTGGATATAG AATTTGATGT ACACCTTGGA ATAGCTCATA CCCGTTGGGC AACACATGGA 
GAACCCAGTC CTGTCAATAG CCACCCCCAG CGCTCTGATA AAAATAATGA ATTTATCGTT 
ATTCACAATG GAATCATCAC CAACTACAAA GACTTGAAAA AGTTTTTGGA AAGCAAAGGC 
TATGACTTCG AATCTGAAAC AGACACAGAG ACAATTGCCA AGCTCGTTAA GTATATGTAT 
GACAATCGGG AAAGTCAAGA TACCAGCTTT ACTACCTTGG TGGAGAGAGT TATCCAACAA 
TTGGAAGGTG CTTTTGCACT TGTGTTTAAA AGTGTTCATT TTCCCGGGCA AGCAGTTGGC 
ACAAGGCGAG GTAGCCCTCT GTTGATTGGT GTACGGAGTG AACATAAACT TTCTACTGAT 
CACATTCCTA TACTCTACAG AACAGCTAGG ACTCAGATTG GATCAAAATT CACACGGTGG 
GGATCACAGG GAGAAAGAGG CAAAGACAAG AAAGGAAGCT GCAATCTCTC TCGTGTGGAC 
AGCACAACCT GCCTTTTCCC GGTGGAAGAA AAAGCAGTGG AGTATTACTT TGCTTCTGAT 
GCAAGTGCTG TCATAGAACA CACCAATCGC GTCATCTTTC TGGAAGATGA TGATGTTGCA 
GCAGTAGTGG ATGGACGTCT TTCTATCCAT CGAATTAAAC GAACTGCAGG AGATCACCCC 
GGACGAGCTG TGCAAACACT CCAGATGGAA CTCCAGCAGA TCATGAAGGG CAACTTCAGT 
TCATTTATGC AGAAGGAAAT ATTTGAGCAG CCAGAGTCTG TCGTGAACAC AATGAGAGGA 
AGAGTCAACT TTGATGACTA TACTGTGAAT TTGGGTGGTT TGAAGGATCA CATAAAGGAG 
ATCCAGAGAT GCCGGCGTTT GATTCTTATT GCTTGTGGAA CAAGTTACCA TGCTGGTGTA 
GCAACACGTC AAGTTCTTGA GGAGCTGACT GAGTTGCCTG TGATGGTGGA ACTAGCAAGT 
GACTTCCTGG ACAGAAACAC ACCAGTCTTT CGAGATGATG TTTGCTTTTT CCTTAGTCAA 
TCAGGTGAGA CAGCAGATAC TTTGATGGGT CTTCGTTACT GTAAGGAGAG AGGAGCTTTA 
ACTGTGGGGA TCACAAACAC AGTTGGCAGT TCCATATCAC GGGAGACAGA TTGTGGAGTT 
CATATTAATG CTGGTCCTGA GATTGGTGTG GCCAGTACAA AGGCTTATAC CAGCCAGTTT 
GTATCCCTTG TGATGTTTGC CCTTATGATG TGTGATGATC GGATCTCCAT GCAAGAAAGA 
CGCAAAGAGA TCATGCTTGG ATTGAAACGG CTGCCTGATT TGATTAAGGA AGTACTGAGC 
ATGGATGACG AAATTCAGAA ACTAGCAACA GAACTTTATC ATCAGAAGTC AGTTCTGATA 
ATGGGACGAG GCTATCATTA TGCTACTTGT CTTGAAGGGG CACTGAAAAT CAAAGAAATT 
ACTTATATGC ACTCTGAAGG CATCCTTGCT GGTGAATTGA AACATGGCCC TCTGGCTTTG 
GTGGATAAAT TGATGCCTGT GATCATGATC ATCATGAGAG ATCACACTTA TGCCAAGTGT 
CAGAATGCTC TTCAGCAAGT GGTTGCTCGG CAGGGGCGGC CTGTGGTAAT TTGTGATAAG 
GAGGATACTG AGACCATTAA GAACACAAAA AGAACGATCA AGGTGCCCCA CTCAGTGGAC 
TGCTTGCAGG GCATTCTCAG CGTGATCCCT TTACAGTTGC TGGCTTTCCA CCTTGCTGTG 
CTGAGAGGCT ATGATGTTGA TTTCCCACGG AATCTTGCCA AATCTGTGAC TGTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian Target of Rapamycin 2 (MTOR2) and C-MYC Modulate Glucosamine-6-Phosphate Synthesis in Glioblastoma (GBM) Cells Through Glutamine: Fructose-6-Phosphate Aminotransferase 1 (GFAT1).
Cellular and molecular neurobiology39(3)415-434(2019 Apr)
Liu B,Huang ZB,Chen X,See YX,Chen ZK,Yao HK

Leukoencephalopathy due to variants in
Neurology92(6)e587-e593(2019 02)
Helman G,Sharma S,Crawford J,Patra B,Jain P,Bent SJ,Urtizberea JA,Saran RK,Taft RJ,van der Knaap MS,Simons C

IL-8-induced O-GlcNAc modification via GLUT3 and GFAT regulates cancer stem cell-like properties in colon and lung cancer cells.
Oncogene38(9)1520-1533(2019 02)
Shimizu M,Tanaka N

GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.
Human molecular genetics27(18)3218-3232(2018 09)
Issop Y,Hathazi D,Khan MM,Rudolf R,Weis J,Spendiff S,Slater CR,Roos A,Lochmüller H

Mutations in GFPT1-related congenital myasthenic syndromes are associated with synaptic morphological defects and underlie a tubular aggregate myopathy with synaptopathy.
Journal of neurology264(8)1791-1803(2017 Aug)
Bauché S,Vellieux G,Sternberg D,Fontenille MJ,De Bruyckere E,Davoine CS,Brochier G,Messéant J,Wolf L,Fardeau M,Lacène E,Romero N,Koenig J,Fournier E,Hantaï D,Streichenberger N,Manel V,Lacour A,Nadaj-Pakleza A,Sukno S,Bouhour F,Laforêt P,Fontaine B,Strochlic L,Eymard B,Chevessier F,Stojkovic T,Nicole S

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GFPT1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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