POLQ cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLQ
Entrez Gene ID	418326
Full Name	DNA polymerase theta
General protein information	Preferred Names DNA polymerase theta Names DNA polymerase theta polymerase (DNA directed), theta polymerase (DNA) theta
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_416549.5	XP_416549.4	DNA polymerase theta
XM_416549.6	XP_416549.5	LOW QUALITY PROTEIN: DNA polymerase theta

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POLQ	XP_545125.3
Gallus gallus (chicken)	POLQ	XP_416549.3
Xenopus tropicalis (tropical clawed frog)	polq	NP_001184156.1
Neurospora crassa	NCU07411	XP_958186.2
Bos taurus (cattle)	POLQ	XP_002684835.1
Rattus norvegicus (Norway rat)	Polq	NP_001099348.1
Danio rerio (zebrafish)	si:rp71-1p14.5	XP_694437.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007533	XP_564726.3
Magnaporthe oryzae (rice blast fungus)	MGG_15295	XP_003711003.1
Homo sapiens (human)	POLQ	NP_955452.3
Macaca mulatta (Rhesus monkey)	POLQ	XP_001111254.2
Drosophila melanogaster (fruit fly)	mus308	NP_524333.1
Pan troglodytes (chimpanzee)	POLQ	XP_001165150.2
Mus musculus (house mouse)	Polq	NP_084253.1

Gene Ontology

The following POLQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa142854	XM_416549.6 Latest version!	Gallus gallus DNA polymerase theta (POLQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa29958	XM_416549.5 Latest version!	Gallus gallus polymerase (DNA directed), theta (POLQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLQ ( XM_416549.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416549.5 Gallus gallus DNA polymerase theta (POLQ), mRNA.

Price & Availability↑

CloneID	OGa142854
Clone ID Related Accession (Same CDS sequence)	XM_416549.6
Accession Version	XM_416549.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	LOW QUALITY PROTEIN: DNA polymerase theta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416549.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCGCGGC GGGAAGATGG CGGCGCCACC GCTAACGGCG TCGCCGCGGT TTCGGCGGCG 
GGGCTCGGCA GGGCGGCCCG GGCTGCCCCG CTCGGCCCCG CGGCTCCCTG CCGCGCCGTG 
CTCAGCCCCC CGCCCGGCCT CGAGGTCTCC CTACGCTGCA GCGGGAGCCC CGGCGGCGGC 
GGCTCGGCAG GGCAATGTCA GCAGGTAAAT GTTCCTGAAG ATCAAGCAGA CAAGTTGCTC 
CTTGCAAGCT GGGGTCTTCC TAAAGCAGTC TTGGAGAAAT ATCACAGTCT GGGAGTAGTA 
CAGATGTTTG AATGGCAAGC AGAGTGCTTA ATGCTTGGAC AAGTTCTGGA AGGGAAGAAT 
CTGGTTTACT CAGCTCCTAC CAGTGCTGGA AAGACTCTTG TTGCAGAACT GCTCATTTTG 
AAGCGAGTCT TAGAGACTCG TAAGAAAGCT CTTTTCATCC TTCCCTTTGT CTCTGTGGCC 
AAAGAGAAGA AATGTTATCT GCAGGCCCTG TTTCAGGAGG TGGATATGAG AGTTGGAGGC 
TACATGGGAA GCATCTCTCC TGCTGGACGT TTCTCTTTGC TGGATGTTGC TGTCTGCACC 
ATTGAGAAAG CCAATGGTCT AATCAATAGG CTAATAGAAG AAAACCAAAT GGACTCACTG 
GGAGTGGTGG TTGTGGATGA GTTGCATATG CTTGGAGACT CTCATCGAGG ATATCTGTTG 
GAACTCCTTC TGACCAAAGT TCGATACATT ACAGAGAAAG CTGCAAAAAG AAAGACAAAG 
ATGAAGAGTC CTGGTTTTGG AGGAATACAG GTAGTAGGCA TGAGTGCTAC CCTCCCAAAC 
TTAGGACTCC TAGCCTCATG GTTAGATGCG GAATTGTATT GTACAGATTT TCGCCCTGTG 
CCCCTCAAGG AGTGGGTGAA AATTGGCAAC AACATTTATG ACTCTTCCAT GAATTTGGTG 
CGAGAATTCC AGCCCAAACT TCAACTGAAG GGAGATGAAG ACCATGTTGT GAGCCTGTGC 
TATGAGACTG TTTGTGATGG CCATTCAGTC CTGCTTTTCT GTCCATCCAA GAATTGGTGT 
GAGAAGCTGG CAGACATCAT TGCCAGGGAA TTCTACAGTT TGCAACAGGC GGAAAGTTCT 
GCAAAAAACT CATCCCTTTC CCCAGTTGTT GTGGACAGAG AAGGTATAGA TGAGGTCCTG 
GATCAGCTAA GGCGCTCTCT CTCAGGTCTG GACTCTGTGC TCCAACGTAC TTTACCGTGG 
GGAGTGGCAT TTCATCATGC AGGTCTTACA TTTGATGAAC GGGACATCAT TGAAGGAGCC 
TTTCGCCAGA GCACGATTAG AGTTCTAGCA GCAACCTCCA CACTCTCCTC TGGAGTGAAC 
CTGCCTGCGC GCAGAGTAAT TATACGAACT CCTATGTTTG GTGGCACACT GCTGGATGTT 
CTCACTTACA AACAGATGGC TGGAAGGGCT GGCAGGAAAG GAGTAGACAC AGAGGGTGAG 
AGCATTTTAG TTTGCAAGCC ATCAGAAAGA TCAAAAGGAA CTGCTCTACT TCAGGGATCT 
CTCAAGCCTG TTCGCAGTTG TTTGCTTAGA AAAGAAGGGG AAGGTGTTGC TTCTAGCATG 
AAAAGAGCAA TACTTGAGAT CATTGTGAGT GGAGTTGCCA ACACTCCAGA TGATGTGCAG 
ACCTATGCCT CTTGCACTCT TCTTGCATCC AGCTTGAAGG ACAACAAGCG AGAAAATGAG 
AAAGAACAAG ACAAAGTGCA GACAGGACCT ATTGAGGCTT GTATAGCATG GCTGCTGGAG 
AATGAATTCA TTCAGGTTCT GGAACGTGCC GATGACAAGA AAGCAAAGAT TTTTCACCCA 
ACACATCTTG GCTCAGCTAC TCTTTCATCT TCTCTTTCGC CAACGGAAGC AATGGAAATT 
TTTTCTGACC TACAGCGAGC TATGAAAAGC TTCGTTCTGG AGAATGATCT GCATATTGTC 
TATCTGGTCA CCCCCGTGTA TGAGGATTGG ACCACAATTG ATTGGTACCA GTTTTTCTGC 
TTATGGGAGA AGCTGCCTGC CTCAATGAAG CGTGTGGCTG AGCTGGTGGG AATTGAAGAA 
GGCTTTCTGG CTCGCTCTGT AAAAGGCAAA ATCACAGCTA AAACAGAAAA ACAGTACAGA 
CAAATGGCCA TCCACAAAAG ATTTTTTACC AGTCTTGCCC TCCTGGATTT GATCAGTGAA 
GTTCCCTTAA AAGATATGAC CAAGAAATAT GGTTGTAGTC GTGGCCAGCT TCAATCCTTA 
CAGCAGTCTG CTGCCACCTA TGCAGGAATG GTGACAGTTT TCTGTAATCG ACTGGGTTGG 
CACAACATGG AGCTGTTGCT CTCCCAGTTC CAGAGCCGCC TCACTTTTGG GGTTCACAGG 
GAGCTTTGTG ACCTGGTACG AGTCTCTCTG CTGAATGCAC AGCGTGCAAG GATGCTTTAC 
AATGCTGGCT TTGTCACAGT TGCTGATCTT GCTAAAGCCA GTCCTGGTGA TGTGGCAACT 
GCTCTGAAGA ATTCAGTACC ATTCAAAAGT GGTCGCAGGG CTGTGGATGA AGATGAAGAA 
TCAGTAGAGG AGCGTCGGAC TGTGCGCAGC ATCTGGATGG CCGGAATGAA GGGCTTAACA 
GAAAGTGAAG CAGCTTCCCT CATTGTGGAG GAAGCCAGGG AACTTCTTCA TCAGGACTTG 
GCAGCAATGG GAGTGCAGTG GAACCCAGAG TCTTTTCTTG AGACATCGTC TATGATCTGC 
AGTGAGTCAG AGATGGAAGA CAGACTGCAT AAGCCAGATG GAGAGCTATC TTCTGAATCT 
TCTGTGATGT TAAACAAAAA AGAGTGCAGA GTTCAGAATC ACAAGGGCCT TGGCTCTCAG 
GCTGGAAACC TATCAAATAT TAAAAAGGGA TATAAAAGGC GACTAAGCAG TAGTACAGCA 
AGCTCTAGAT TTGAGAGTGA AGTCATATAC AGGAAACAGG CTCAAGCAGA TGAAAGAAAT 
GATGATACTG TGCACAGTGC TAGAAAACAG CCCAATATGA GCCGAGGCAA TGAAAATGTG 
AAAGGAATTC TTAAGGTCGA AAACAAGGCA GATTCTGAAA GAGCAAGTCC AGAAGTCCAT 
ACTGAGCAAG GAAAGACACC AACGTTAGCA AAAATGTCTT CAGCCTCTAG GTTGTTAAGA 
AGTGAGGACA CACGTTTAAA CCAGACTAGG AACAAAAATA CTTCTTCAAA ACTGCAGAAG 
TCACTGCTTG AAGATTCAAG TATGGTTATC ATGGACATTA AAAAACCTTC TGCTTTCCTC 
CCCAGCATTT CTAAAGGTAA CTTATTTTCA GACCAAAACA ATAAGGAATG TATAGAAGAA 
GTGTCTGCAA CTCATAAGGT CTCAAATTCT GTACAAATGA GAGCAAATAA TGTAGGGACA 
AAAGGTAAAA TGGAGAGTAT ATTTACAGAA CTTTCTAACT CCAATGAAGA CATGCCTATG 
GAGAGAGCAG GTTTTCAGGC AACTGCTACA TTACAGAGTA TTCCACACAT CAACAAGGAG 
ACACATGATA CTGTAGAAAG TCCAGTAATA CCTTCTAGGG CTAGAATACA GAGAAATAAA 
ACTACTGCTG ATAAGCAGAA TAAAATCAAT AAAATGCAAA CAGATGTGAG CTTTGCTGAG 
TTGAAGGTTG ATAATCAGCA TACTGCTCTA GATGCAGGTC ATTGTAATGT AAACACTAAC 
TGCTCTGGAA ATGTGTGCGT CCATAAAGGC AGTAGGAAAC AGAAATCTGT GTACTTTTGT 
CCTAGTGGTG ATAAATTCTA CCATGCTCAG ATCCAGAATG GTGGTATAAA TGGGAAAAAA 
ATACCCAATG GAAAATTGTT ACAAGATGAA GCACTACAAA AAGGCAACAG TCATCCTAAG 
GATTTTCTGA AAGACTCTCT AGTAAAATCC AAATCTATAT GTGATGCAGA TGATATTCAG 
AATGGTCCGT CCTCTCGTTT GATTTGTGTC TCTATGGACT TTGAAGATAG CTTCCAGTTG 
GATACTCAAA CTGAGGGGAT AATAAAACAG GAGGCAGCAG CTGAAATCAC ACGACAGCAG 
GGAATACAGG ATCCAGAACT GTCAGTGGCA CCTCAGCAGG AAATCTCCAT GAATACAAGC 
ACTTTGCAGG CTGGGAATGC TACTGGGGAA AGGCTAGATG CAACAGTAAG GAAACTAGGC 
TTATCGAGTA TTATCACAAC AAATAATGTT ATGAAAAACA CAGATCATCA CTGCAGTAAC 
AAAGTTTTAG AGTCTGGAGA CCTTAGTGTA CAGCCTGTAG CAAAAGAAAT AGCATCTGTA 
CCTGTCCTTA AAGGAAGTGA CTACTCAGTG ACCGATTCTC AGCTAAACAG TTTTCTACAG 
TATTATCATA CCCAAAATTC AGTGAAAGGG GAGAGTGCTT CTAACAGTCC CAATAAAGCA 
ACCTGCCCTC TTGATAGGGA GCACTTTGTA GAAGACGAAC ACCATCCTGT CATGGAAACA 
AGCCTGAATA CAAGTGATGG CTTGTTGTTT GATGATAGCT TCAGTAATGT CAGTGATCTT 
TCAGCTGTGA ACATCATGGA AGCTGACAGA AAGGAACCTG AGGGGAGGCA GGGAGCTTCA 
CTTCCTGCAG GTGGTCTTTT GCAGAACCTA AGGCCTGTTC AGAATAAATC TTATGTCAGT 
GACAATCGGA GGGAGCATGA GGATCCAGCA CAGAATAATG ATACATCTCT AGTATTCTCT 
GAACTCATAG CTGAAGTTTT AGATCATGCA AACTCCCCAA CTTTTTTGGG AGATCTTCCT 
TGTACATCTC ACAGTCAGTC TAAATTAAGA AACATGGAGA TTTGTGAGAA TGACTCCAAT 
CCAAAGGATT TGGAAGTGGA TCATGGTAAA AAGCTCCAGT GTAGCCAGCA AATGTTAGCT 
AAGAAAACCC ATCCATTAGC ATGGACCAAC CACTCATTTG AACTGAGCCC AGGACTTCAG 
GATGTACTAG ACAAATGGCC TAGTCCTTCA GGCACTGGAC TTGCTCAAGT AAATGTAAGA 
AGCTCCTGTC TGAAAGAAAA TCTAGCTGTT TCATTTGCTA ATCAAGAGCC AGATGCAGTT 
TTTGAATTTG ACCATCATCA GCTAGAGCCT TTGCCTAGTT GTCAGGATTT AAAAGACTGT 
TGCGAAGTTA AAGAAAACAA AACAAGAGAC TTGGATATTC AGAAATCAGA CTGTGTAACA 
CTTATGCTCA GGGGAAGCAA CAGGACATCC TATCCTCCAC CAGATAATTG CAATGGGTTT 
GTTCCTCCAA CACCCCCCAA AGAACTTTTT CCAAAAAGTT CTGTATCTTT CTCTGAGAAA 
TCTGCAAGGA AGAGACAGAT TGCCAGCATG AATGAAGAGC AGCTAGTTCT GCCACAGCAC 
AATTGCAAGA CTGATGCAGA GCAACAGATA GTTGAGAGAG TAGCCTCAAC TGAGGCTGAT 
GAGATAGAAG ATGATTCCAC GATAGACAAA GACTTTTCAC TGCAGCTGTC TCAGGATGTT 
TTTCCACTCA CTCCCTCAAG TACTGAAGGG TTCACTATTG TTGATGTAGC AGGTGACAAA 
ACACTTTTCC AGACTTTTCT TGCAGAATGG AAGGAGAAAA GCAGATTCTC CATCTCAGTG 
GCATGTGAAA GAAGAAAATG TCTTTTGTTA ACCAAATCAA CGATTGGTGG CAGATTCAAA 
CAAGGAAATT CTCCCCGGAG GATGCAGGTC AAAGATGATG GATTTCCTAT CCAACACTGT 
GAAGACACCC TGGTAGTTGG ACTGGCAGTC TGTTGGGGTG GAAAAGATGC CTACTATATA 
TCTTTGCAGC AGATGCAAGA TCAGACTGAA ATCTGTCCAA GTTTGGCACC ACCACCACTG 
GACAAAAACC TATCTGTAAC GGAGAGACTG CGTCATCTGC AGCTTTATCT GCAGGAGAAG 
CAGGAGCGCT CACTTGTCAT GTATAACTTC ATTCAGCACT ATAAGACCCT GGTGATGGCT 
TGTGGCATCT CCTTGGAAGG AAGTTTTGAG GACCCAAAGG TTGCATGTTG GCTGCTTGAT 
TCTGGCTCTA AGGAATGTAC CCTTCACAAC ATGGTGACTA ACTTTCTACC CAATGAGCTA 
CCTCTGCTAG AAGGGGTAGG CACTGGCCAG GGAGTGCAGA GCCTGGGGCT TAGCTCCAGT 
GAAGATCATT CTGGGCGGTA CAGAGCAGCA ATAGAGTCTG TGCTCATCTT CAACATCATG 
AACCAACTCC ATAGTGAACT ACGGAAGGAA AATCTGACAG ATGTGTTTTC TAAAGTGGAG 
ATGCCCAACC ATTATTGCTT GGCTCTGCTG GAGCTGAATG GCATTGGTTT TAGCACAGCA 
GCATATGAAA CCCAGAAACA GGTCATGCAA GCTAAACTAA CTGAGATTGA GACCAAGGCT 
TATCAACTGG CAGGCCACAG TTTCTCCTTG ACCAGCCCTG ATGATGTTGC AGAGGTTTTG 
TTCCTAGAGT TGAAGTTGCC ACAAAATGGA GATGTGAAAG TTCAAGGGAA CAAGAAAACT 
CTGGGTTACA CCAGAAGAAC AAGTATTAAA GGAAACCGGG TTAGGTTGAG CAAGCAGTTC 
AGTACAACAA AGGATGTTTT GGAGAAATTA AAGCCACTTC ACCCATTGCC AGGGCTCATA 
CTGGAATGGA GAAGAATCAA CAGTGCCATT TCTAAAGTGG TGTTTCCACT ACAGAGGGAA 
AAACGATTCA ATTCTGCACT AGGAATGGAA AGGATATATC CAGTGTCACA GACACACACA 
GCTACAGGTC GAGTAAGTTT CGCAGAGCCA AATATCCAGA ATGTGCCAAG AGATTTTGAG 
ATTGAAATGC CGATTGTAGT TGAAGAAAGC CCACCCTTCC GAACCCATGT TTATTCTTGT 
GCTGTTTCCA GGGTCAGAGG TAGAAAGCAT TCCAGTGTCT TGCCTAGTGG TGTAAATACT 
TGGACTGCAG ACAGAACTGA AAGAAGAGGA ATCCCATTTN TTGTTAGCAT GCGGCATGCC 
TTTGTTCCCT TCCCAGGTGG TTTTATCTTG GCTGCTGATT ATTGTCAGCT TGAATTGAGG 
ATCCTTGCAC ATTTCTCTCG TGACTGTCGA CTCATTGAAG CCTTTAACAA GGGTGCCGAT 
GTCTTTAAGA GCATCGCAGC AGAATGGAAG ATGATTGATT CTGAAGCTGT TGGAGACAGC 
ACCAGGCAAC AAGCTAAGCA GATTTGCTAT GGAATCATCT ATGGAATAGG AGCAAAATCT 
TTGGGTGAGC AGATGGGCAT TGATGACAAC AAAGCTGCCA ATTACATCGA ATCCTTCAAA 
TCAAGATACA CAGGTATTCA GAAATTCTTG AAGGAGACAG TAAGTAACTG CAGAAGAGAT 
GGGTTTGTGC AGACCATACT GGGAAGACGG AGATACCTGC CATCTATCAG AGATTCAAAT 
CCCTACATTA AAGCTCACGC AGAGCGCCAG GCTGTGAACA CAACTGTGCA GGGATCTGCT 
GCTGACATTG TCAAAACAGC CACTGTGAAC ATTCAGAGAC GCCTGGAAGC TCTCTCTTCT 
GTGAACAAGT CCCATGGACA TCTGGAGAAC TCATTCCAGA GAGATAAGAC AGGAAGGCTG 
TCAAGGAAGA GAAACAGAGA GATGTTGCAT CCCATCAGTG GAGGTTTCTT TATCCTTCAG 
CTCCATGATG AGCTCCTCTA TGAAGTTGCA GAAGATGATG TGATCCAGGT CGCTCAGATT 
GTCAAGCATG AGATGGAAAA TGCTGTCAAA CTCTCAGTGA AACTGAATGT TAAAGTGAAA 
ATTGGACCTA GCTGGGGAGA GCTGCAGGAC CTGGAGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416549.5
CDS	16..7857
Translation	MARREDGGATANGVAAVSAAGLGRAARAAPLGPAAPCRAVLSPPPGLEVSLRCSGSPGGGGSAGQCQQVNVPEDQADKLLLASWGLPKAVLEKYHSLGVVQMFEWQAECLMLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQALFQEVDMRVGGYMGSISPAGRFSLLDVAVCTIEKANGLINRLIEENQMDSLGVVVVDELHMLGDSHRGYLLELLLTKVRYITEKAAKRKTKMKSPGFGGIQVVGMSATLPNLGLLASWLDAELYCTDFRPVPLKEWVKIGNNIYDSSMNLVREFQPKLQLKGDEDHVVSLCYETVCDGHSVLLFCPSKNWCEKLADIIAREFYSLQQAESSAKNSSLSPVVVDREGIDEVLDQLRRSLSGLDSVLQRTLPWGVAFHHAGLTFDERDIIEGAFRQSTIRVLAATSTLSSGVNLPARRVIIRTPMFGGTLLDVLTYKQMAGRAGRKGVDTEGESILVCKPSERSKGTALLQGSLKPVRSCLLRKEGEGVASSMKRAILEIIVSGVANTPDDVQTYASCTLLASSLKDNKRENEKEQDKVQTGPIEACIAWLLENEFIQVLERADDKKAKIFHPTHLGSATLSSSLSPTEAMEIFSDLQRAMKSFVLENDLHIVYLVTPVYEDWTTIDWYQFFCLWEKLPASMKRVAELVGIEEGFLARSVKGKITAKTEKQYRQMAIHKRFFTSLALLDLISEVPLKDMTKKYGCSRGQLQSLQQSAATYAGMVTVFCNRLGWHNMELLLSQFQSRLTFGVHRELCDLVRVSLLNAQRARMLYNAGFVTVADLAKASPGDVATALKNSVPFKSGRRAVDEDEESVEERRTVRSIWMAGMKGLTESEAASLIVEEARELLHQDLAAMGVQWNPESFLETSSMICSESEMEDRLHKPDGELSSESSVMLNKKECRVQNHKGLGSQAGNLSNIKKGYKRRLSSSTASSRFESEVIYRKQAQADERNDDTVHSARKQPNMSRGNENVKGILKVENKADSERASPEVHTEQGKTPTLAKMSSASRLLRSEDTRLNQTRNKNTSSKLQKSLLEDSSMVIMDIKKPSAFLPSISKGNLFSDQNNKECIEEVSATHKVSNSVQMRANNVGTKGKMESIFTELSNSNEDMPMERAGFQATATLQSIPHINKETHDTVESPVIPSRARIQRNKTTADKQNKINKMQTDVSFAELKVDNQHTALDAGHCNVNTNCSGNVCVHKGSRKQKSVYFCPSGDKFYHAQIQNGGINGKKIPNGKLLQDEALQKGNSHPKDFLKDSLVKSKSICDADDIQNGPSSRLICVSMDFEDSFQLDTQTEGIIKQEAAAEITRQQGIQDPELSVAPQQEISMNTSTLQAGNATGERLDATVRKLGLSSIITTNNVMKNTDHHCSNKVLESGDLSVQPVAKEIASVPVLKGSDYSVTDSQLNSFLQYYHTQNSVKGESASNSPNKATCPLDREHFVEDEHHPVMETSLNTSDGLLFDDSFSNVSDLSAVNIMEADRKEPEGRQGASLPAGGLLQNLRPVQNKSYVSDNRREHEDPAQNNDTSLVFSELIAEVLDHANSPTFLGDLPCTSHSQSKLRNMEICENDSNPKDLEVDHGKKLQCSQQMLAKKTHPLAWTNHSFELSPGLQDVLDKWPSPSGTGLAQVNVRSSCLKENLAVSFANQEPDAVFEFDHHQLEPLPSCQDLKDCCEVKENKTRDLDIQKSDCVTLMLRGSNRTSYPPPDNCNGFVPPTPPKELFPKSSVSFSEKSARKRQIASMNEEQLVLPQHNCKTDAEQQIVERVASTEADEIEDDSTIDKDFSLQLSQDVFPLTPSSTEGFTIVDVAGDKTLFQTFLAEWKEKSRFSISVACERRKCLLLTKSTIGGRFKQGNSPRRMQVKDDGFPIQHCEDTLVVGLAVCWGGKDAYYISLQQMQDQTEICPSLAPPPLDKNLSVTERLRHLQLYLQEKQERSLVMYNFIQHYKTLVMACGISLEGSFEDPKVACWLLDSGSKECTLHNMVTNFLPNELPLLEGVGTGQGVQSLGLSSSEDHSGRYRAAIESVLIFNIMNQLHSELRKENLTDVFSKVEMPNHYCLALLELNGIGFSTAAYETQKQVMQAKLTEIETKAYQLAGHSFSLTSPDDVAEVLFLELKLPQNGDVKVQGNKKTLGYTRRTSIKGNRVRLSKQFSTTKDVLEKLKPLHPLPGLILEWRRINSAISKVVFPLQREKRFNSALGMERIYPVSQTHTATGRVSFAEPNIQNVPRDFEIEMPIVVEESPPFRTHVYSCAVSRVRGRKHSSVLPSGVNTWTADRTERRGIPFnullVSMRHAFVPFPGGFILAADYCQLELRILAHFSRDCRLIEAFNKGADVFKSIAAEWKMIDSEAVGDSTRQQAKQICYGIIYGIGAKSLGEQMGIDDNKAANYIESFKSRYTGIQKFLKETVSNCRRDGFVQTILGRRRYLPSIRDSNPYIKAHAERQAVNTTVQGSAADIVKTATVNIQRRLEALSSVNKSHGHLENSFQRDKTGRLSRKRNREMLHPISGGFFILQLHDELLYEVAEDDVIQVAQIVKHEMENAVKLSVKLNVKVKIGPSWGELQDLEL

Target ORF information:

RefSeq Version	XM_416549.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase theta (POLQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416549.6

ORF Insert Sequence:

ATGGCGCGGC GGGAAGATGG CGGCGCCACC GCTAACGGCG TCGCCGCGGT TTCGGCGGCG 
GGGCTCGGCA GGGCGGCCCG GGCTGCCCCG CTCGGCCCCG CGGCTCCCTG CCGCGCCGTG 
CTCAGCCCCC CGCCCGGCCT CGAGGTCTCC CTACGCTGCA GCGGGAGCCC CGGCGGCGGC 
GGCTCGGCAG GGCAATGTCA GCAGGTAAAT GTTCCTGAAG ATCAAGCAGA CAAGTTGCTC 
CTTGCAAGCT GGGGTCTTCC TAAAGCAGTC TTGGAGAAAT ATCACAGTCT GGGAGTAGTA 
CAGATGTTTG AATGGCAAGC AGAGTGCTTA ATGCTTGGAC AAGTTCTGGA AGGGAAGAAT 
CTGGTTTACT CAGCTCCTAC CAGTGCTGGA AAGACTCTTG TTGCAGAACT GCTCATTTTG 
AAGCGAGTCT TAGAGACTCG TAAGAAAGCT CTTTTCATCC TTCCCTTTGT CTCTGTGGCC 
AAAGAGAAGA AATGTTATCT GCAGGCCCTG TTTCAGGAGG TGGATATGAG AGTTGGAGGC 
TACATGGGAA GCATCTCTCC TGCTGGACGT TTCTCTTTGC TGGATGTTGC TGTCTGCACC 
ATTGAGAAAG CCAATGGTCT AATCAATAGG CTAATAGAAG AAAACCAAAT GGACTCACTG 
GGAGTGGTGG TTGTGGATGA GTTGCATATG CTTGGAGACT CTCATCGAGG ATATCTGTTG 
GAACTCCTTC TGACCAAAGT TCGATACATT ACAGAGAAAG CTGCAAAAAG AAAGACAAAG 
ATGAAGAGTC CTGGTTTTGG AGGAATACAG GTAGTAGGCA TGAGTGCTAC CCTCCCAAAC 
TTAGGACTCC TAGCCTCATG GTTAGATGCG GAATTGTATT GTACAGATTT TCGCCCTGTG 
CCCCTCAAGG AGTGGGTGAA AATTGGCAAC AACATTTATG ACTCTTCCAT GAATTTGGTG 
CGAGAATTCC AGCCCAAACT TCAACTGAAG GGAGATGAAG ACCATGTTGT GAGCCTGTGC 
TATGAGACTG TTTGTGATGG CCATTCAGTC CTGCTTTTCT GTCCATCCAA GAATTGGTGT 
GAGAAGCTGG CAGACATCAT TGCCAGGGAA TTCTACAGTT TGCAACAGGC GGAAAGTTCT 
GCAAAAAACT CATCCCTTTC CCCAGTTGTT GTGGACAGAG AAGGTATAGA TGAGGTCCTG 
GATCAGCTAA GGCGCTCTCT CTCAGGTCTG GACTCTGTGC TCCAACGTAC TTTACCGTGG 
GGAGTGGCAT TTCATCATGC AGGTCTTACA TTTGATGAAC GGGACATCAT TGAAGGAGCC 
TTTCGCCAGA GCACGATTAG AGTTCTAGCA GCAACCTCCA CACTCTCCTC TGGAGTGAAC 
CTGCCTGCGC GCAGAGTAAT TATACGAACT CCTATGTTTG GTGGCACACT GCTGGATGTT 
CTCACTTACA AACAGATGGC TGGAAGGGCT GGCAGGAAAG GAGTAGACAC AGAGGGTGAG 
AGCATTTTAG TTTGCAAGCC ATCAGAAAGA TCAAAAGGAA CTGCTCTACT TCAGGGATCT 
CTCAAGCCTG TTCGCAGTTG TTTGCTTAGA AAAGAAGGGG AAGGTGTTGC TTCTAGCATG 
AAAAGAGCAA TACTTGAGAT CATTGTGAGT GGAGTTGCCA ACACTCCAGA TGATGTGCAG 
ACCTATGCCT CTTGCACTCT TCTTGCATCC AGCTTGAAGG ACAACAAGCG AGAAAATGAG 
AAAGAACAAG ACAAAGTGCA GACAGGACCT ATTGAGGCTT GTATAGCATG GCTGCTGGAG 
AATGAATTCA TTCAGGTTCT GGAACGTGCC GATGACAAGA AAGCAAAGAT TTTTCACCCA 
ACACATCTTG GCTCAGCTAC TCTTTCATCT TCTCTTTCGC CAACGGAAGC AATGGAAATT 
TTTTCTGACC TACAGCGAGC TATGAAAAGC TTCGTTCTGG AGAATGATCT GCATATTGTC 
TATCTGGTCA CCCCCGTGTA TGAGGATTGG ACCACAATTG ATTGGTACCA GTTTTTCTGC 
TTATGGGAGA AGCTGCCTGC CTCAATGAAG CGTGTGGCTG AGCTGGTGGG AATTGAAGAA 
GGCTTTCTGG CTCGCTCTGT AAAAGGCAAA ATCACAGCTA AAACAGAAAA ACAGTACAGA 
CAAATGGCCA TCCACAAAAG ATTTTTTACC AGTCTTGCCC TCCTGGATTT GATCAGTGAA 
GTTCCCTTAA AAGATATGAC CAAGAAATAT GGTTGTAGTC GTGGCCAGCT TCAATCCTTA 
CAGCAGTCTG CTGCCACCTA TGCAGGAATG GTGACAGTTT TCTGTAATCG ACTGGGTTGG 
CACAACATGG AGCTGTTGCT CTCCCAGTTC CAGAGCCGCC TCACTTTTGG GGTTCACAGG 
GAGCTTTGTG ACCTGGTACG AGTCTCTCTG CTGAATGCAC AGCGTGCAAG GATGCTTTAC 
AATGCTGGCT TTGTCACAGT TGCTGATCTT GCTAAAGCCA GTCCTGGTGA TGTGGCAACT 
GCTCTGAAGA ATTCAGTACC ATTCAAAAGT GGTCGCAGGG CTGTGGATGA AGATGAAGAA 
TCAGTAGAGG AGCGTCGGAC TGTGCGCAGC ATCTGGATGG CCGGAATGAA GGGCTTAACA 
GAAAGTGAAG CAGCTTCCCT CATTGTGGAG GAAGCCAGGG AACTTCTTCA TCAGGACTTG 
GCAGCAATGG GAGTGCAGTG GAACCCAGAG TCTTTTCTTG AGACATCGTC TATGATCTGC 
AGTGAGTCAG AGATGGAAGA CAGACTGCAT AAGCCAGATG GAGAGCTATC TTCTGAATCT 
TCTGTGATGT TAAACAAAAA AGAGTGCAGA GTTCAGAATC ACAAGGGCCT TGGCTCTCAG 
GCTGGAAACC TATCAAATAT TAAAAAGGGA TATAAAAGGC GACTAAGCAG TAGTACAGCA 
AGCTCTAGAT TTGAGAGTGA AGTCATATAC AGGAAACAGG CTCAAGCAGA TGAAAGAAAT 
GATGATACTG TGCACAGTGC TAGAAAACAG CCCAATATGA GCCGAGGCAA TGAAAATGTG 
AAAGGAATTC TTAAGGTCGA AAACAAGGCA GATTCTGAAA GAGCAAGTCC AGAAGTCCAT 
ACTGAGCAAG GAAAGACACC AACGTTAGCA AAAATGTCTT CAGCCTCTAG GTTGTTAAGA 
AGTGAGGACA CACGTTTAAA CCAGACTAGG AACAAAAATA CTTCTTCAAA ACTGCAGAAG 
TCACTGCTTG AAGATTCAAG TATGGTTATC ATGGACATTA AAAAACCTTC TGCTTTCCTC 
CCCAGCATTT CTAAAGGTAA CTTATTTTCA GACCAAAACA ATAAGGAATG TATAGAAGAA 
GTGTCTGCAA CTCATAAGGT CTCAAATTCT GTACAAATGA GAGCAAATAA TGTAGGGACA 
AAAGGTAAAA TGGAGAGTAT ATTTACAGAA CTTTCTAACT CCAATGAAGA CATGCCTATG 
GAGAGAGCAG GTTTTCAGGC AACTGCTACA TTACAGAGTA TTCCACACAT CAACAAGGAG 
ACACATGATA CTGTAGAAAG TCCAGTAATA CCTTCTAGGG CTAGAATACA GAGAAATAAA 
ACTACTGCTG ATAAGCAGAA TAAAATCAAT AAAATGCAAA CAGATGTGAG CTTTGCTGAG 
TTGAAGGTTG ATAATCAGCA TACTGCTCTA GATGCAGGTC ATTGTAATGT AAACACTAAC 
TGCTCTGGAA ATGTGTGCGT CCATAAAGGC AGTAGGAAAC AGAAATCTGT GTACTTTTGT 
CCTAGTGGTG ATAAATTCTA CCATGCTCAG ATCCAGAATG GTGGTATAAA TGGGAAAAAA 
ATACCCAATG GAAAATTGTT ACAAGATGAA GCACTACAAA AAGGCAACAG TCATCCTAAG 
GATTTTCTGA AAGACTCTCT AGTAAAATCC AAATCTATAT GTGATGCAGA TGATATTCAG 
AATGGTCCGT CCTCTCGTTT GATTTGTGTC TCTATGGACT TTGAAGATAG CTTCCAGTTG 
GATACTCAAA CTGAGGGGAT AATAAAACAG GAGGCAGCAG CTGAAATCAC ACGACAGCAG 
GGAATACAGG ATCCAGAACT GTCAGTGGCA CCTCAGCAGG AAATCTCCAT GAATACAAGC 
ACTTTGCAGG CTGGGAATGC TACTGGGGAA AGGCTAGATG CAACAGTAAG GAAACTAGGC 
TTATCGAGTA TTATCACAAC AAATAATGTT ATGAAAAACA CAGATCATCA CTGCAGTAAC 
AAAGTTTTAG AGTCTGGAGA CCTTAGTGTA CAGCCTGTAG CAAAAGAAAT AGCATCTGTA 
CCTGTCCTTA AAGGAAGTGA CTACTCAGTG ACCGATTCTC AGCTAAACAG TTTTCTACAG 
TATTATCATA CCCAAAATTC AGTGAAAGGG GAGAGTGCTT CTAACAGTCC CAATAAAGCA 
ACCTGCCCTC TTGATAGGGA GCACTTTGTA GAAGACGAAC ACCATCCTGT CATGGAAACA 
AGCCTGAATA CAAGTGATGG CTTGTTGTTT GATGATAGCT TCAGTAATGT CAGTGATCTT 
TCAGCTGTGA ACATCATGGA AGCTGACAGA AAGGAACCTG AGGGGAGGCA GGGAGCTTCA 
CTTCCTGCAG GTGGTCTTTT GCAGAACCTA AGGCCTGTTC AGAATAAATC TTATGTCAGT 
GACAATCGGA GGGAGCATGA GGATCCAGCA CAGAATAATG ATACATCTCT AGTATTCTCT 
GAACTCATAG CTGAAGTTTT AGATCATGCA AACTCCCCAA CTTTTTTGGG AGATCTTCCT 
TGTACATCTC ACAGTCAGTC TAAATTAAGA AACATGGAGA TTTGTGAGAA TGACTCCAAT 
CCAAAGGATT TGGAAGTGGA TCATGGTAAA AAGCTCCAGT GTAGCCAGCA AATGTTAGCT 
AAGAAAACCC ATCCATTAGC ATGGACCAAC CACTCATTTG AACTGAGCCC AGGACTTCAG 
GATGTACTAG ACAAATGGCC TAGTCCTTCA GGCACTGGAC TTGCTCAAGT AAATGTAAGA 
AGCTCCTGTC TGAAAGAAAA TCTAGCTGTT TCATTTGCTA ATCAAGAGCC AGATGCAGTT 
TTTGAATTTG ACCATCATCA GCTAGAGCCT TTGCCTAGTT GTCAGGATTT AAAAGACTGT 
TGCGAAGTTA AAGAAAACAA AACAAGAGAC TTGGATATTC AGAAATCAGA CTGTGTAACA 
CTTATGCTCA GGGGAAGCAA CAGGACATCC TATCCTCCAC CAGATAATTG CAATGGGTTT 
GTTCCTCCAA CACCCCCCAA AGAACTTTTT CCAAAAAGTT CTGTATCTTT CTCTGAGAAA 
TCTGCAAGGA AGAGACAGAT TGCCAGCATG AATGAAGAGC AGCTAGTTCT GCCACAGCAC 
AATTGCAAGA CTGATGCAGA GCAACAGATA GTTGAGAGAG TAGCCTCAAC TGAGGCTGAT 
GAGATAGAAG ATGATTCCAC GATAGACAAA GACTTTTCAC TGCAGCTGTC TCAGGATGTT 
TTTCCACTCA CTCCCTCAAG TACTGAAGGG TTCACTATTG TTGATGTAGC AGGTGACAAA 
ACACTTTTCC AGACTTTTCT TGCAGAATGG AAGGAGAAAA GCAGATTCTC CATCTCAGTG 
GCATGTGAAA GAAGAAAATG TCTTTTGTTA ACCAAATCAA CGATTGGTGG CAGATTCAAA 
CAAGGAAATT CTCCCCGGAG GATGCAGGTC AAAGATGATG GATTTCCTAT CCAACACTGT 
GAAGACACCC TGGTAGTTGG ACTGGCAGTC TGTTGGGGTG GAAAAGATGC CTACTATATA 
TCTTTGCAGC AGATGCAAGA TCAGACTGAA ATCTGTCCAA GTTTGGCACC ACCACCACTG 
GACAAAAACC TATCTGTAAC GGAGAGACTG CGTCATCTGC AGCTTTATCT GCAGGAGAAG 
CAGGAGCGCT CACTTGTCAT GTATAACTTC ATTCAGCACT ATAAGACCCT GGTGATGGCT 
TGTGGCATCT CCTTGGAAGG AAGTTTTGAG GACCCAAAGG TTGCATGTTG GCTGCTTGAT 
TCTGGCTCTA AGGAATGTAC CCTTCACAAC ATGGTGACTA ACTTTCTACC CAATGAGCTA 
CCTCTGCTAG AAGGGGTAGG CACTGGCCAG GGAGTGCAGA GCCTGGGGCT TAGCTCCAGT 
GAAGATCATT CTGGGCGGTA CAGAGCAGCA ATAGAGTCTG TGCTCATCTT CAACATCATG 
AACCAACTCC ATAGTGAACT ACGGAAGGAA AATCTGACAG ATGTGTTTTC TAAAGTGGAG 
ATGCCCAACC ATTATTGCTT GGCTCTGCTG GAGCTGAATG GCATTGGTTT TAGCACAGCA 
GCATATGAAA CCCAGAAACA GGTCATGCAA GCTAAACTAA CTGAGATTGA GACCAAGGCT 
TATCAACTGG CAGGCCACAG TTTCTCCTTG ACCAGCCCTG ATGATGTTGC AGAGGTTTTG 
TTCCTAGAGT TGAAGTTGCC ACAAAATGGA GATGTGAAAG TTCAAGGGAA CAAGAAAACT 
CTGGGTTACA CCAGAAGAAC AAGTATTAAA GGAAACCGGG TTAGGTTGAG CAAGCAGTTC 
AGTACAACAA AGGATGTTTT GGAGAAATTA AAGCCACTTC ACCCATTGCC AGGGCTCATA 
CTGGAATGGA GAAGAATCAA CAGTGCCATT TCTAAAGTGG TGTTTCCACT ACAGAGGGAA 
AAACGATTCA ATTCTGCACT AGGAATGGAA AGGATATATC CAGTGTCACA GACACACACA 
GCTACAGGTC GAGTAAGTTT CGCAGAGCCA AATATCCAGA ATGTGCCAAG AGATTTTGAG 
ATTGAAATGC CGATTGTAGT TGAAGAAAGC CCACCCTTCC GAACCCATGT TTATTCTTGT 
GCTGTTTCCA GGGTCAGAGG TAGAAAGCAT TCCAGTGTCT TGCCTAGTGG TGTAAATACT 
TGGACTGCAG ACAGAACTGA AAGAAGAGGA ATCCCATTTN TTGTTAGCAT GCGGCATGCC 
TTTGTTCCCT TCCCAGGTGG TTTTATCTTG GCTGCTGATT ATTGTCAGCT TGAATTGAGG 
ATCCTTGCAC ATTTCTCTCG TGACTGTCGA CTCATTGAAG CCTTTAACAA GGGTGCCGAT 
GTCTTTAAGA GCATCGCAGC AGAATGGAAG ATGATTGATT CTGAAGCTGT TGGAGACAGC 
ACCAGGCAAC AAGCTAAGCA GATTTGCTAT GGAATCATCT ATGGAATAGG AGCAAAATCT 
TTGGGTGAGC AGATGGGCAT TGATGACAAC AAAGCTGCCA ATTACATCGA ATCCTTCAAA 
TCAAGATACA CAGGTATTCA GAAATTCTTG AAGGAGACAG TAAGTAACTG CAGAAGAGAT 
GGGTTTGTGC AGACCATACT GGGAAGACGG AGATACCTGC CATCTATCAG AGATTCAAAT 
CCCTACATTA AAGCTCACGC AGAGCGCCAG GCTGTGAACA CAACTGTGCA GGGATCTGCT 
GCTGACATTG TCAAAACAGC CACTGTGAAC ATTCAGAGAC GCCTGGAAGC TCTCTCTTCT 
GTGAACAAGT CCCATGGACA TCTGGAGAAC TCATTCCAGA GAGATAAGAC AGGAAGGCTG 
TCAAGGAAGA GAAACAGAGA GATGTTGCAT CCCATCAGTG GAGGTTTCTT TATCCTTCAG 
CTCCATGATG AGCTCCTCTA TGAAGTTGCA GAAGATGATG TGATCCAGGT CGCTCAGATT 
GTCAAGCATG AGATGGAAAA TGCTGTCAAA CTCTCAGTGA AACTGAATGT TAAAGTGAAA 
ATTGGACCTA GCTGGGGAGA GCTGCAGGAC CTGGAGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_416549.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416549.4 Gallus gallus polymerase (DNA directed), theta (POLQ), mRNA.

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CloneID	OGa29958
Clone ID Related Accession (Same CDS sequence)	XM_416549.5
Accession Version	XM_416549.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	DNA polymerase theta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455728.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416549.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAT GGCAAGCAGA GTGCTTAATG CTTGGACAAG TTCTGGAAGG GAAGAATCTG 
GTTTACTCAG CTCCTACCAG TGCTGGAAAG ACTCTTGTTG CAGAACTGCT CATTTTGAAG 
CGAGTCTTAG AGACTCGTAA GAAAGCTCTT TTCATCCTTC CCTTTGTCTC TGTGGCCAAA 
GAGAAGAAAT GTTATCTGCA GGCCCTGTTT CAGGAGGTGG ATATGAGAGT TGGAGGCTAC 
ATGGGAAGCA TCTCTCCTGC TGGACGTTTC TCTTTGCTGG ATGTTGCTGT CTGCACCATT 
GAGAAAGCCA ATGGTCTAAT CAATAGGCTA ATAGAAGAAA ACCAAATGGA CTCACTGGGA 
GTGGTGGTTG TGGATGAGTT GCATATGCTT GGAGACTCTC ATCGAGGATA TCTGTTGGAA 
CTCCTTCTGA CCAAAGTTCG ATACATTACA GAGAAAGCTG CAAAAAGAAA GACAAAGATG 
AAGAGTCCTG GTTTTGGAGG AATACAGGTA GTAGGCATGA GTGCTACCCT CCCAAACTTA 
GGACTCCTAG CCTCATGGTT AGACGCGGAA TTGTATTGTA CAGATTTTCG CCCTGTGCCC 
CTCAAGGAGT GGGTGAAAAT TGGCAACAAC ATTTATGACT CTTCCATGAA TTTGGTGCGA 
GAATTCCAGC CCAAACTTCA ACTGAAGGGA GATGAAGACC ATGTTGTGAG CCTGTGCTAT 
GAGACTGTTT GTGATGGCCA TTCAGTCCTG CTTTTCTGTC CATCCAAGAA TTGGTGTGAG 
AAGCTGGCAG ACATCATTGC CAGGGAATTC TACAGTTTGC AACAGGCGGA AAGTTCTGCA 
AAAAACTCAT CCCTTTCCCC AGTTGTTGTG GACAGAGAAG GTATAGACGA GGTCCTGGAT 
CAGCTAAGAC GCTCTCTCTC AGGTCTGGAC TCTGTGCTCC AACGTACTTT ACCGTGGGGA 
GTGGCATTTC ATCATGCAGG TCTTACATTT GATGAACGGG ACATCATTGA AGGAGCCTTT 
CGCCAGAGCA CGATTAGAGT TCTAGCAGCA ACCTCCACAC TCTCCTCTGG AGTGAACCTG 
CCTGCGCGCA GAGTAATTAT ACGAACTCCT ATGTTTGGTG GCACACTGCT GGATGTTCTC 
ACTTACAAAC AGATGGCTGG AAGGGCTGGC AGGAAAGGAG TAGACACAGA GGGTGAGAGC 
ATTTTAGTTT GCAAGCCATC AGAAAGATCA AAAGGAACTG CTCTACTTCA GGGATCTCTC 
AAGCCTGTTC GCAGTTGTTT GCTTAGAAAA GAAGGGGAAG GTGTTGCTTC TAGCATGAAA 
AGAGCAATAC TTGAGATCAT TGTGAGTGGA GTTGCCAACA CTCCAGATGA TGTGCAGACC 
TATGCCTCTT GCACTCTTCT TGCATCCAGC TTGAAGGACA ACAAGCGAGA AAATGAGAAA 
GAACAAGACA AAGTGCAGAC AGGACCTATT GAGGCTTGTA TAGCATGGCT GCTGGAGAAT 
GAATTCATTC AGGTTCTGGA ACGTGCCGAT GACAAGAAAG CAAAGATTTT TCACCCAACA 
CATCTTGGCT CAGCTACTCT TTCATCTTCT CTTTCGCCAA CGGAAGCAAT GGAAATTTTT 
TCTGACCTAC AGCGAGCTAT GAAAAGCTTC GTTCTGGAGA ATGATCTGCA TATTGTCTAT 
CTGGTCACCC CCGTGTATGA GGATTGGACC ACAATTGATT GGTACCAGTT TTTCTGCTTA 
TGGGAGAAGC TGCCTGCCTC AATGAAGCGT GTGGCTGAGC TGGTGGGAAT TGAAGAAGGC 
TTTCTGGCTC GCTCTGTAAA AGGCAAAATC ACAGCTAAAA CAGAAAAACA GTACAGACAA 
ATGGCCATCC ACAAAAGATT TTTTACCAGT CTTGCCCTCC TGGATTTGAT CAGTGAAGTT 
CCCTTAAAAG ATATGACCAA GAAATATGGT TGTAGTCGTG GCCAGCTTCA ATCCTTACAG 
CAGTCTGCTG CCACCTATGC AGGAATGGTG ACAGTTTTCT GTAATCGACT GGGTTGGCAC 
AACATGGAGC TGTTGCTCTC CCAGTTCCAG AGCCGCCTCA CTTTTGGGGT TCACAGGGAG 
CTTTGTGACC TGGTACGAGT CTCTCTGCTG AATGCACAGC GTGCAAGGAT GCTTTACAAT 
GCTGGCTTTG TCACAGTTGC TGATCTTGCT AAAGCCAGTC CTGGTGATGT GGCAACTGCT 
CTGAAGAATT CAGTACCATT CAAAAGTGGT CGCAGGGCTG TGGATGAAGA TGAAGAATCA 
GTAGAGGAGC GTCGGACTGT GCGCAGCATC TGGATGGCCG GAATGAAGGG CTTAACAGAA 
AGTGAAGCAG CTTCCCTCAT TGTGGAGGAA GCCAGGGAAC TTCTTCATCA GGACTTGGCA 
GCAATGGGAG TGCAGTGGAA CCCAGAGTCT TTTCTTGAGA CATCGTCTAT GATCTGCAGT 
GAGTCAGAGA TGGAAGACAG ACTGCATAAG CCAGATGGAG AGCTATCTTC TGAATCTTCT 
GTGATGTTAA ACAAAAAAGA GTGCAGAGTT CAGAATCACA AGGGCCTTGG CTCTCAGGCT 
GGAAACCTAT CAAATATTAA AAAGGGATAT AAAAGGCGAC TAAGCAGTAG TACAGCAAGC 
TCTAGATTTG AGAGTGAAGT CATATACAGG AAACAGGCTC AAGCAGATGA AAGAAATGAT 
GATACTGTGC ACAGTGCTAG AAAACAGCCC AATATGAGCC GAGGCAATGA AAATGTGAAA 
GGAATTCTTA AGGTCGAAAA CAAGGCAGAT TCTGAAAGAG CAAGTCCAGA AGTCCATACT 
GAGCAAGGAA AGACACCAAC GTTAGCAAAA ATGTCTTCAG CCTCTAGGTT GTTAAGAAGT 
GAGGACACAC GTTTAAACCA GACTAGGAAC AAAAATACTT CTTCAAAACT GCAGAAGTCA 
CTGCTTGAAG ATTCAAGTAT GGTTATCATG GACATTAAAA AACCTTCTGC TTTCCTCCCC 
AGCATTTCTA AAGGTAACTT ATTTTCAGAC CAAAACAATA AGGAATGTAT AGAAGAAGTG 
TCTGCAACTC ATAAGGTCTC AAATTCTGTA CAAATGAGAG CAAATAATGT AGGGACAAAA 
GGTAAAATGG AGAGTATATT TACAGAACTT TCTAACTCCA ATGAAGACAT GCCTATGGAG 
AGAGCAGGTT TTCAGGCAAC TGCTACATTA CAGAGTATTC CACACATCAA CAAGGAGACA 
CATGATACTG TAGAAAGTCC AGTAATACCT TCTAGGGCTA GAATACAGAG AAATAAAACT 
ACTGCTGATA AGCAGAATAA AATCAATAAA ATGCAAACAG ATGTGAGCTT TGCTGAGTTG 
AAGGTTGATA ATCAGCATAC TGCTCTAGAT GCAGGTCATT GTAATGTAAA CACTAACTGC 
TCTGGAAATG TGTGCGTCCA TAAAGGCAGT AGGAAACAGA AATCTGTGTA CTTTTGTCCT 
AGTGGTGATA AATTCTACCA TGCTCAGATC CAGAATGGTG GTATAAATGG GAAAAAAATA 
CCCAATGGAA AATTGTTACA AGATGAAGCA CTACAAAAAG GCAACAGTCA TCCTAAGGAT 
TTTCTGAAAG ACTCTCTAGT AAAATCCAAA TCTATATGTG ATGCAGATGA TATTCAGAAT 
GGTCCGTCCT CTCGTTTGAT TTGTGTCTCT ATGGACTTTG AAGATAGCTT CCAGTTGGAT 
ACTCAAACTG AGGGGATAAT AAAACAGGAG GCAGCAGCTG AAATCACACG ACAGCAGGGA 
ATACAGGATC CAGAACTGTC AGTGGCACCT CAGCAGGAAA TCTCCATGAA TACAAGCACT 
TTGCAGGCTG GGAATGCTAC TGGGGAAAGG CTAGATGCAA CAGTAAGGAA ACTAGGCTTA 
TCGAGTATTA TCACAACAAA TAATGTTATG AAAAACACAG ATCATCACTG CAGTAACAAA 
GTTTTAGAGT CTGGAGACCT TAGTGTACAG CCTGTAGCAA AAGAAATAGC ATCTGTACCT 
GTCCTTAAAG GAAGTGACTA CTCAGTGACC GATTCTCAGC TAAACAGTTT TCTACAGTAT 
TATCATACCC AAAATTCAGT GAAAGGGGAG AGTGCTTCTA ACAGTCCCAA TAAAGCAACC 
TGCCCTCTTG ATAGGGAGCA CTTTGTAGAA GACGAACACC ATCCTGTCAT GGAAACAAGC 
CTGAATACAA GTGATGGCTT GTTGTTTGAT GATAGCTTCA GTAATGTCAG TGATCTTTCA 
GCTGTGAACA TCATGGAAGC TGACAGAAAG GAACCTGAGG GGAGGCAGGG AGCTTCACTT 
CCTGCAGGTG GTCTTTTGCA GAACCTAAGG CCTGTTCAGA ATAAATCTTA TGTCAGTGAC 
AATCGGAGGG AGCATGAGGA TCCAGCACAG AATAATGATA CATCTCTAGT ATTCTCTGAA 
CTCATAGCTG AAGTTTTAGA TCATGCAAAC TCCCCAACTT TTTTGGGAGA TCTTCCTTGT 
ACATCTCACA GTCAGTCTAA ATTAAGAAAC ATGGAGATTT GTGAGAATGA CTCCAATCCA 
AAGGATTTGG AAGTGGATCA TGGTAAAAAG CTCCAGTGTA GCCAGCAAAT GTTAGCTAAG 
AAAACCCATC CATTAGCATG GACCAACCAC TCATTTGAAC TGAGCCCAGG ACTTCAGGAT 
GTACTAGACA AATGGCCTAG TCCTTCAGGC ACTGGACTTG CTCAAGTAAA TGTAAGAAGC 
TCCTGTCTGA AAGAAAATCT AGCTGTTTCA TTTGCTAATC AAGAGCCAGA TGCAGTTTTT 
GAATTTGACC ATCATCAGCT AGAGCCTTTG CCTAGTTGTC AGGATTTAAA AGACTGTTGC 
GAAGTTAAAG AAAACAAAAC AAGAGACTTG GATATTCAGA AATCAGACTG TGTAACACTT 
ATGCTCAGGG GAAGCAACAG GACATCCTAT CCTCCACCAG ATAATTGCAA TGGGTTTGTT 
CCTCCAACGC CCCCCAAAGA ACTTTTTCCA AAAAGTTCTG TATCTTTCTC TGAGAAATCT 
GCAAGGAAGA GACAGATTGC CAGCATGAAT GAAGAGCAGC TAGTTCTGCC ACAGCACAAT 
TGCAAGACTG ATGCAGAGCA ACAGATAGTT GAGAGAGTAG CCTCAACTGA GGCTGATGAG 
ATAGAAGATG ATTCCACGAT AGACAAAGAC TTTTCACTGC AGCTGTCTCA GGATGTTTTT 
CCACTCACTC CCTCAAGTAC TGAAGGGTTC ACTATTGTTG ATGTAGCAGG TGACAAAACA 
CTTTTCCAGA CTTTTCTTGC AGAATGGAAG GAGAAAAGCA GATTCTCCAT CTCAGTGGCA 
TGTGAAAGAA GAAAATGTCT TTTGTTAACC AAATCAACGA TTGGTGGCAG ATTCAAACAA 
GGAAATTCTC CCCAGAGGAT GCAGGTCAAA GATGATGGAT TTCCTATCCA ACACTGTGAA 
GACACCCTGG TAGTTGGACT GGCAGTCTGT TGGGGTGGAA AAGATGCCTA CTATATATCT 
TTGCAGCAGA TGCAAGATCA GACTGAAATC TGTCCAAGTT TGGCACCACC ACCACTGGAC 
AAAAACCTAT CTGTAACGGA GAGACTGCGT CATCTGCAGC TTTATCTGCA GGAGAAGCAG 
GAGCGCTCAC TTGTCATGTA TAACTTCATT CAGCACTATA AGACCCTGGT GATGGCTTGT 
GGCATCTCCT TGGAAGGAAG TTTTGAGGAC CCAAAGGTTG CATGTTGGCT GCTTGATTCT 
GGCTCTAAGG AATGTACCCT TCACAACATG GTGACTAACT TTCTACCCAA TGAGCTACCT 
CTGCTAGAAG GGGTAGGCAC TGGCCAGGGA GTGCAGAGCC TGGGGCTTAG CTCCAGTGAA 
GATCATTCTG GGCGGTACAG AGCAGCAATA GAGTCTGTGC TCATCTTCAA CATCATGAAC 
CAACTCCATA GTGAACTACG GAAGGAAAAT CTGACAGATG TGTTTTCTAA AGTGGAGATG 
CCCAACCATT ATTGCTTGGC TCTGCTGGAG CTGAATGGCA TTGGTTTTAG CACAGCAGCA 
TATGAAACCC AGAAACAGGT CATGCAAGCT AAACTAACTG AGATTGAGAC CAAGGCTTAT 
CAACTGGCAG GCCACAGTTT CTCCTTGACC AGCCCTGATG ATGTTGCAGA GGTTTTGTTC 
CTAGAGTTGA AGTTGCCACA AAATGGAGAT GTGAAAGTTC AAGGGAACAA GAAAACTCTG 
GGTTACACCA GAAGAACAAG TATTAAAGGA AACCGGGTTA GGTTGAGCAA GCAGTTCAGT 
ACAACAAAGG ATGTTTTGGA GAAATTAAAG CCACTTCACC CATTGCCAGG GCTCATACTG 
GAATGGAGAA GAATCAACAG TGCCATTTCT AAAGTGGTGT TTCCACTACA GAGGGAAAAA 
CGATTCAATT CTGCACTAGG AATGGAAAGG ATATATCCAG TGTCACAGAC ACACACAGCT 
ACAGGTCGAG TAAGTTTCGC AGAGCCAAAT ATCCAGAATG TGCCAAGAGA TTTTGAGATT 
GAAATGCCGA TTGTAGTTGA AGAAAGCCCA CCCTTCCGAA CCCATGTTTA TTCTTGTGCT 
GTTTCCAGGG TCAGAGGTAG AAAGCATTCC AGTGTCTTGC CTAGTGGTGT AAATACTTGG 
ACTGAAGACA GAACTGAAAG AAGAGGAAGC CCATTTTCTG TTAGCATGCG GCATGCCTTT 
GTTCCCTTCC CAGGTGGTTT TATCTTGGCT GCTGATTATT GTCAGCTTGA ATTGAGGATC 
CTTGCACATT TCTCTCGTGA CTGTCGACTC ATTGAAGCCT TTAACAAGGG TGCCGATGTC 
TTTAAGAGCA TCGCAGCAGA ATGGAAGATG ATTGATTCTG AAGCTGTTGG AGACAGCACC 
AGGCAACAAG CTAAGCAGAT TTGCTATGGA ATCATCTATG GAATAGGAGC AAAATCTTTG 
GGTGAGCAGA TGGGCATTGA TGACAACAAA GCTGCCAATT ACATCGAATC CTTCAAATCA 
AGATACACAG GTATTCAGAA ATTCTTGAAG GAGACAGTAA GTAACTGCAG AAGAGATGGG 
TTTGTGCAGA CCATACTGGG AAGACGGAGA TACCTGCCAT CTATCAGAGA TTCAAATCCC 
TACATTAAAG CTCACGCAGA GCGCCAGGCT GTGAACACAA CTGTGCAGGG ATCTGCTGCT 
GACATTGTCA AAACAGCCAC TGTGAACATT CAGAGACGCC TGGAAGCTCT CTCTTCTGTG 
AACAAGTCCC ATGGACATCT GGAGAACTCA TTCCAGAGAG ATAAGACAGG AAGGCTGTCA 
AGGAAGAGAA ACAGAGAGAT GTTGCATCCC ATCAGTGGAG GTTTCTTTAT CCTTCAGCTC 
CATGATGAGC TCCTCTATGA AGTTGCAGAA GATGATGTGA TCCAGGTCGC TCAGATTGTC 
AAGCATGAGA TGGAAAATGC TGTCAAACTC TCAGTGAAAC TGAATGTTAA AGTGAAAATT 
GGACCTAGCT GGGGAGAGCT GCAGGACCTG GAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416549.4
CDS	294..7832
Translation	MFEWQAECLMLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQALFQEVDMRVGGYMGSISPAGRFSLLDVAVCTIEKANGLINRLIEENQMDSLGVVVVDELHMLGDSHRGYLLELLLTKVRYITEKAAKRKTKMKSPGFGGIQVVGMSATLPNLGLLASWLDAELYCTDFRPVPLKEWVKIGNNIYDSSMNLVREFQPKLQLKGDEDHVVSLCYETVCDGHSVLLFCPSKNWCEKLADIIAREFYSLQQAESSAKNSSLSPVVVDREGIDEVLDQLRRSLSGLDSVLQRTLPWGVAFHHAGLTFDERDIIEGAFRQSTIRVLAATSTLSSGVNLPARRVIIRTPMFGGTLLDVLTYKQMAGRAGRKGVDTEGESILVCKPSERSKGTALLQGSLKPVRSCLLRKEGEGVASSMKRAILEIIVSGVANTPDDVQTYASCTLLASSLKDNKRENEKEQDKVQTGPIEACIAWLLENEFIQVLERADDKKAKIFHPTHLGSATLSSSLSPTEAMEIFSDLQRAMKSFVLENDLHIVYLVTPVYEDWTTIDWYQFFCLWEKLPASMKRVAELVGIEEGFLARSVKGKITAKTEKQYRQMAIHKRFFTSLALLDLISEVPLKDMTKKYGCSRGQLQSLQQSAATYAGMVTVFCNRLGWHNMELLLSQFQSRLTFGVHRELCDLVRVSLLNAQRARMLYNAGFVTVADLAKASPGDVATALKNSVPFKSGRRAVDEDEESVEERRTVRSIWMAGMKGLTESEAASLIVEEARELLHQDLAAMGVQWNPESFLETSSMICSESEMEDRLHKPDGELSSESSVMLNKKECRVQNHKGLGSQAGNLSNIKKGYKRRLSSSTASSRFESEVIYRKQAQADERNDDTVHSARKQPNMSRGNENVKGILKVENKADSERASPEVHTEQGKTPTLAKMSSASRLLRSEDTRLNQTRNKNTSSKLQKSLLEDSSMVIMDIKKPSAFLPSISKGNLFSDQNNKECIEEVSATHKVSNSVQMRANNVGTKGKMESIFTELSNSNEDMPMERAGFQATATLQSIPHINKETHDTVESPVIPSRARIQRNKTTADKQNKINKMQTDVSFAELKVDNQHTALDAGHCNVNTNCSGNVCVHKGSRKQKSVYFCPSGDKFYHAQIQNGGINGKKIPNGKLLQDEALQKGNSHPKDFLKDSLVKSKSICDADDIQNGPSSRLICVSMDFEDSFQLDTQTEGIIKQEAAAEITRQQGIQDPELSVAPQQEISMNTSTLQAGNATGERLDATVRKLGLSSIITTNNVMKNTDHHCSNKVLESGDLSVQPVAKEIASVPVLKGSDYSVTDSQLNSFLQYYHTQNSVKGESASNSPNKATCPLDREHFVEDEHHPVMETSLNTSDGLLFDDSFSNVSDLSAVNIMEADRKEPEGRQGASLPAGGLLQNLRPVQNKSYVSDNRREHEDPAQNNDTSLVFSELIAEVLDHANSPTFLGDLPCTSHSQSKLRNMEICENDSNPKDLEVDHGKKLQCSQQMLAKKTHPLAWTNHSFELSPGLQDVLDKWPSPSGTGLAQVNVRSSCLKENLAVSFANQEPDAVFEFDHHQLEPLPSCQDLKDCCEVKENKTRDLDIQKSDCVTLMLRGSNRTSYPPPDNCNGFVPPTPPKELFPKSSVSFSEKSARKRQIASMNEEQLVLPQHNCKTDAEQQIVERVASTEADEIEDDSTIDKDFSLQLSQDVFPLTPSSTEGFTIVDVAGDKTLFQTFLAEWKEKSRFSISVACERRKCLLLTKSTIGGRFKQGNSPQRMQVKDDGFPIQHCEDTLVVGLAVCWGGKDAYYISLQQMQDQTEICPSLAPPPLDKNLSVTERLRHLQLYLQEKQERSLVMYNFIQHYKTLVMACGISLEGSFEDPKVACWLLDSGSKECTLHNMVTNFLPNELPLLEGVGTGQGVQSLGLSSSEDHSGRYRAAIESVLIFNIMNQLHSELRKENLTDVFSKVEMPNHYCLALLELNGIGFSTAAYETQKQVMQAKLTEIETKAYQLAGHSFSLTSPDDVAEVLFLELKLPQNGDVKVQGNKKTLGYTRRTSIKGNRVRLSKQFSTTKDVLEKLKPLHPLPGLILEWRRINSAISKVVFPLQREKRFNSALGMERIYPVSQTHTATGRVSFAEPNIQNVPRDFEIEMPIVVEESPPFRTHVYSCAVSRVRGRKHSSVLPSGVNTWTEDRTERRGSPFSVSMRHAFVPFPGGFILAADYCQLELRILAHFSRDCRLIEAFNKGADVFKSIAAEWKMIDSEAVGDSTRQQAKQICYGIIYGIGAKSLGEQMGIDDNKAANYIESFKSRYTGIQKFLKETVSNCRRDGFVQTILGRRRYLPSIRDSNPYIKAHAERQAVNTTVQGSAADIVKTATVNIQRRLEALSSVNKSHGHLENSFQRDKTGRLSRKRNREMLHPISGGFFILQLHDELLYEVAEDDVIQVAQIVKHEMENAVKLSVKLNVKVKIGPSWGELQDLEL

Target ORF information:

RefSeq Version	XM_416549.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus polymerase (DNA directed), theta (POLQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416549.5

ORF Insert Sequence:

ATGTTTGAAT GGCAAGCAGA GTGCTTAATG CTTGGACAAG TTCTGGAAGG GAAGAATCTG 
GTTTACTCAG CTCCTACCAG TGCTGGAAAG ACTCTTGTTG CAGAACTGCT CATTTTGAAG 
CGAGTCTTAG AGACTCGTAA GAAAGCTCTT TTCATCCTTC CCTTTGTCTC TGTGGCCAAA 
GAGAAGAAAT GTTATCTGCA GGCCCTGTTT CAGGAGGTGG ATATGAGAGT TGGAGGCTAC 
ATGGGAAGCA TCTCTCCTGC TGGACGTTTC TCTTTGCTGG ATGTTGCTGT CTGCACCATT 
GAGAAAGCCA ATGGTCTAAT CAATAGGCTA ATAGAAGAAA ACCAAATGGA CTCACTGGGA 
GTGGTGGTTG TGGATGAGTT GCATATGCTT GGAGACTCTC ATCGAGGATA TCTGTTGGAA 
CTCCTTCTGA CCAAAGTTCG ATACATTACA GAGAAAGCTG CAAAAAGAAA GACAAAGATG 
AAGAGTCCTG GTTTTGGAGG AATACAGGTA GTAGGCATGA GTGCTACCCT CCCAAACTTA 
GGACTCCTAG CCTCATGGTT AGACGCGGAA TTGTATTGTA CAGATTTTCG CCCTGTGCCC 
CTCAAGGAGT GGGTGAAAAT TGGCAACAAC ATTTATGACT CTTCCATGAA TTTGGTGCGA 
GAATTCCAGC CCAAACTTCA ACTGAAGGGA GATGAAGACC ATGTTGTGAG CCTGTGCTAT 
GAGACTGTTT GTGATGGCCA TTCAGTCCTG CTTTTCTGTC CATCCAAGAA TTGGTGTGAG 
AAGCTGGCAG ACATCATTGC CAGGGAATTC TACAGTTTGC AACAGGCGGA AAGTTCTGCA 
AAAAACTCAT CCCTTTCCCC AGTTGTTGTG GACAGAGAAG GTATAGACGA GGTCCTGGAT 
CAGCTAAGAC GCTCTCTCTC AGGTCTGGAC TCTGTGCTCC AACGTACTTT ACCGTGGGGA 
GTGGCATTTC ATCATGCAGG TCTTACATTT GATGAACGGG ACATCATTGA AGGAGCCTTT 
CGCCAGAGCA CGATTAGAGT TCTAGCAGCA ACCTCCACAC TCTCCTCTGG AGTGAACCTG 
CCTGCGCGCA GAGTAATTAT ACGAACTCCT ATGTTTGGTG GCACACTGCT GGATGTTCTC 
ACTTACAAAC AGATGGCTGG AAGGGCTGGC AGGAAAGGAG TAGACACAGA GGGTGAGAGC 
ATTTTAGTTT GCAAGCCATC AGAAAGATCA AAAGGAACTG CTCTACTTCA GGGATCTCTC 
AAGCCTGTTC GCAGTTGTTT GCTTAGAAAA GAAGGGGAAG GTGTTGCTTC TAGCATGAAA 
AGAGCAATAC TTGAGATCAT TGTGAGTGGA GTTGCCAACA CTCCAGATGA TGTGCAGACC 
TATGCCTCTT GCACTCTTCT TGCATCCAGC TTGAAGGACA ACAAGCGAGA AAATGAGAAA 
GAACAAGACA AAGTGCAGAC AGGACCTATT GAGGCTTGTA TAGCATGGCT GCTGGAGAAT 
GAATTCATTC AGGTTCTGGA ACGTGCCGAT GACAAGAAAG CAAAGATTTT TCACCCAACA 
CATCTTGGCT CAGCTACTCT TTCATCTTCT CTTTCGCCAA CGGAAGCAAT GGAAATTTTT 
TCTGACCTAC AGCGAGCTAT GAAAAGCTTC GTTCTGGAGA ATGATCTGCA TATTGTCTAT 
CTGGTCACCC CCGTGTATGA GGATTGGACC ACAATTGATT GGTACCAGTT TTTCTGCTTA 
TGGGAGAAGC TGCCTGCCTC AATGAAGCGT GTGGCTGAGC TGGTGGGAAT TGAAGAAGGC 
TTTCTGGCTC GCTCTGTAAA AGGCAAAATC ACAGCTAAAA CAGAAAAACA GTACAGACAA 
ATGGCCATCC ACAAAAGATT TTTTACCAGT CTTGCCCTCC TGGATTTGAT CAGTGAAGTT 
CCCTTAAAAG ATATGACCAA GAAATATGGT TGTAGTCGTG GCCAGCTTCA ATCCTTACAG 
CAGTCTGCTG CCACCTATGC AGGAATGGTG ACAGTTTTCT GTAATCGACT GGGTTGGCAC 
AACATGGAGC TGTTGCTCTC CCAGTTCCAG AGCCGCCTCA CTTTTGGGGT TCACAGGGAG 
CTTTGTGACC TGGTACGAGT CTCTCTGCTG AATGCACAGC GTGCAAGGAT GCTTTACAAT 
GCTGGCTTTG TCACAGTTGC TGATCTTGCT AAAGCCAGTC CTGGTGATGT GGCAACTGCT 
CTGAAGAATT CAGTACCATT CAAAAGTGGT CGCAGGGCTG TGGATGAAGA TGAAGAATCA 
GTAGAGGAGC GTCGGACTGT GCGCAGCATC TGGATGGCCG GAATGAAGGG CTTAACAGAA 
AGTGAAGCAG CTTCCCTCAT TGTGGAGGAA GCCAGGGAAC TTCTTCATCA GGACTTGGCA 
GCAATGGGAG TGCAGTGGAA CCCAGAGTCT TTTCTTGAGA CATCGTCTAT GATCTGCAGT 
GAGTCAGAGA TGGAAGACAG ACTGCATAAG CCAGATGGAG AGCTATCTTC TGAATCTTCT 
GTGATGTTAA ACAAAAAAGA GTGCAGAGTT CAGAATCACA AGGGCCTTGG CTCTCAGGCT 
GGAAACCTAT CAAATATTAA AAAGGGATAT AAAAGGCGAC TAAGCAGTAG TACAGCAAGC 
TCTAGATTTG AGAGTGAAGT CATATACAGG AAACAGGCTC AAGCAGATGA AAGAAATGAT 
GATACTGTGC ACAGTGCTAG AAAACAGCCC AATATGAGCC GAGGCAATGA AAATGTGAAA 
GGAATTCTTA AGGTCGAAAA CAAGGCAGAT TCTGAAAGAG CAAGTCCAGA AGTCCATACT 
GAGCAAGGAA AGACACCAAC GTTAGCAAAA ATGTCTTCAG CCTCTAGGTT GTTAAGAAGT 
GAGGACACAC GTTTAAACCA GACTAGGAAC AAAAATACTT CTTCAAAACT GCAGAAGTCA 
CTGCTTGAAG ATTCAAGTAT GGTTATCATG GACATTAAAA AACCTTCTGC TTTCCTCCCC 
AGCATTTCTA AAGGTAACTT ATTTTCAGAC CAAAACAATA AGGAATGTAT AGAAGAAGTG 
TCTGCAACTC ATAAGGTCTC AAATTCTGTA CAAATGAGAG CAAATAATGT AGGGACAAAA 
GGTAAAATGG AGAGTATATT TACAGAACTT TCTAACTCCA ATGAAGACAT GCCTATGGAG 
AGAGCAGGTT TTCAGGCAAC TGCTACATTA CAGAGTATTC CACACATCAA CAAGGAGACA 
CATGATACTG TAGAAAGTCC AGTAATACCT TCTAGGGCTA GAATACAGAG AAATAAAACT 
ACTGCTGATA AGCAGAATAA AATCAATAAA ATGCAAACAG ATGTGAGCTT TGCTGAGTTG 
AAGGTTGATA ATCAGCATAC TGCTCTAGAT GCAGGTCATT GTAATGTAAA CACTAACTGC 
TCTGGAAATG TGTGCGTCCA TAAAGGCAGT AGGAAACAGA AATCTGTGTA CTTTTGTCCT 
AGTGGTGATA AATTCTACCA TGCTCAGATC CAGAATGGTG GTATAAATGG GAAAAAAATA 
CCCAATGGAA AATTGTTACA AGATGAAGCA CTACAAAAAG GCAACAGTCA TCCTAAGGAT 
TTTCTGAAAG ACTCTCTAGT AAAATCCAAA TCTATATGTG ATGCAGATGA TATTCAGAAT 
GGTCCGTCCT CTCGTTTGAT TTGTGTCTCT ATGGACTTTG AAGATAGCTT CCAGTTGGAT 
ACTCAAACTG AGGGGATAAT AAAACAGGAG GCAGCAGCTG AAATCACACG ACAGCAGGGA 
ATACAGGATC CAGAACTGTC AGTGGCACCT CAGCAGGAAA TCTCCATGAA TACAAGCACT 
TTGCAGGCTG GGAATGCTAC TGGGGAAAGG CTAGATGCAA CAGTAAGGAA ACTAGGCTTA 
TCGAGTATTA TCACAACAAA TAATGTTATG AAAAACACAG ATCATCACTG CAGTAACAAA 
GTTTTAGAGT CTGGAGACCT TAGTGTACAG CCTGTAGCAA AAGAAATAGC ATCTGTACCT 
GTCCTTAAAG GAAGTGACTA CTCAGTGACC GATTCTCAGC TAAACAGTTT TCTACAGTAT 
TATCATACCC AAAATTCAGT GAAAGGGGAG AGTGCTTCTA ACAGTCCCAA TAAAGCAACC 
TGCCCTCTTG ATAGGGAGCA CTTTGTAGAA GACGAACACC ATCCTGTCAT GGAAACAAGC 
CTGAATACAA GTGATGGCTT GTTGTTTGAT GATAGCTTCA GTAATGTCAG TGATCTTTCA 
GCTGTGAACA TCATGGAAGC TGACAGAAAG GAACCTGAGG GGAGGCAGGG AGCTTCACTT 
CCTGCAGGTG GTCTTTTGCA GAACCTAAGG CCTGTTCAGA ATAAATCTTA TGTCAGTGAC 
AATCGGAGGG AGCATGAGGA TCCAGCACAG AATAATGATA CATCTCTAGT ATTCTCTGAA 
CTCATAGCTG AAGTTTTAGA TCATGCAAAC TCCCCAACTT TTTTGGGAGA TCTTCCTTGT 
ACATCTCACA GTCAGTCTAA ATTAAGAAAC ATGGAGATTT GTGAGAATGA CTCCAATCCA 
AAGGATTTGG AAGTGGATCA TGGTAAAAAG CTCCAGTGTA GCCAGCAAAT GTTAGCTAAG 
AAAACCCATC CATTAGCATG GACCAACCAC TCATTTGAAC TGAGCCCAGG ACTTCAGGAT 
GTACTAGACA AATGGCCTAG TCCTTCAGGC ACTGGACTTG CTCAAGTAAA TGTAAGAAGC 
TCCTGTCTGA AAGAAAATCT AGCTGTTTCA TTTGCTAATC AAGAGCCAGA TGCAGTTTTT 
GAATTTGACC ATCATCAGCT AGAGCCTTTG CCTAGTTGTC AGGATTTAAA AGACTGTTGC 
GAAGTTAAAG AAAACAAAAC AAGAGACTTG GATATTCAGA AATCAGACTG TGTAACACTT 
ATGCTCAGGG GAAGCAACAG GACATCCTAT CCTCCACCAG ATAATTGCAA TGGGTTTGTT 
CCTCCAACGC CCCCCAAAGA ACTTTTTCCA AAAAGTTCTG TATCTTTCTC TGAGAAATCT 
GCAAGGAAGA GACAGATTGC CAGCATGAAT GAAGAGCAGC TAGTTCTGCC ACAGCACAAT 
TGCAAGACTG ATGCAGAGCA ACAGATAGTT GAGAGAGTAG CCTCAACTGA GGCTGATGAG 
ATAGAAGATG ATTCCACGAT AGACAAAGAC TTTTCACTGC AGCTGTCTCA GGATGTTTTT 
CCACTCACTC CCTCAAGTAC TGAAGGGTTC ACTATTGTTG ATGTAGCAGG TGACAAAACA 
CTTTTCCAGA CTTTTCTTGC AGAATGGAAG GAGAAAAGCA GATTCTCCAT CTCAGTGGCA 
TGTGAAAGAA GAAAATGTCT TTTGTTAACC AAATCAACGA TTGGTGGCAG ATTCAAACAA 
GGAAATTCTC CCCAGAGGAT GCAGGTCAAA GATGATGGAT TTCCTATCCA ACACTGTGAA 
GACACCCTGG TAGTTGGACT GGCAGTCTGT TGGGGTGGAA AAGATGCCTA CTATATATCT 
TTGCAGCAGA TGCAAGATCA GACTGAAATC TGTCCAAGTT TGGCACCACC ACCACTGGAC 
AAAAACCTAT CTGTAACGGA GAGACTGCGT CATCTGCAGC TTTATCTGCA GGAGAAGCAG 
GAGCGCTCAC TTGTCATGTA TAACTTCATT CAGCACTATA AGACCCTGGT GATGGCTTGT 
GGCATCTCCT TGGAAGGAAG TTTTGAGGAC CCAAAGGTTG CATGTTGGCT GCTTGATTCT 
GGCTCTAAGG AATGTACCCT TCACAACATG GTGACTAACT TTCTACCCAA TGAGCTACCT 
CTGCTAGAAG GGGTAGGCAC TGGCCAGGGA GTGCAGAGCC TGGGGCTTAG CTCCAGTGAA 
GATCATTCTG GGCGGTACAG AGCAGCAATA GAGTCTGTGC TCATCTTCAA CATCATGAAC 
CAACTCCATA GTGAACTACG GAAGGAAAAT CTGACAGATG TGTTTTCTAA AGTGGAGATG 
CCCAACCATT ATTGCTTGGC TCTGCTGGAG CTGAATGGCA TTGGTTTTAG CACAGCAGCA 
TATGAAACCC AGAAACAGGT CATGCAAGCT AAACTAACTG AGATTGAGAC CAAGGCTTAT 
CAACTGGCAG GCCACAGTTT CTCCTTGACC AGCCCTGATG ATGTTGCAGA GGTTTTGTTC 
CTAGAGTTGA AGTTGCCACA AAATGGAGAT GTGAAAGTTC AAGGGAACAA GAAAACTCTG 
GGTTACACCA GAAGAACAAG TATTAAAGGA AACCGGGTTA GGTTGAGCAA GCAGTTCAGT 
ACAACAAAGG ATGTTTTGGA GAAATTAAAG CCACTTCACC CATTGCCAGG GCTCATACTG 
GAATGGAGAA GAATCAACAG TGCCATTTCT AAAGTGGTGT TTCCACTACA GAGGGAAAAA 
CGATTCAATT CTGCACTAGG AATGGAAAGG ATATATCCAG TGTCACAGAC ACACACAGCT 
ACAGGTCGAG TAAGTTTCGC AGAGCCAAAT ATCCAGAATG TGCCAAGAGA TTTTGAGATT 
GAAATGCCGA TTGTAGTTGA AGAAAGCCCA CCCTTCCGAA CCCATGTTTA TTCTTGTGCT 
GTTTCCAGGG TCAGAGGTAG AAAGCATTCC AGTGTCTTGC CTAGTGGTGT AAATACTTGG 
ACTGAAGACA GAACTGAAAG AAGAGGAAGC CCATTTTCTG TTAGCATGCG GCATGCCTTT 
GTTCCCTTCC CAGGTGGTTT TATCTTGGCT GCTGATTATT GTCAGCTTGA ATTGAGGATC 
CTTGCACATT TCTCTCGTGA CTGTCGACTC ATTGAAGCCT TTAACAAGGG TGCCGATGTC 
TTTAAGAGCA TCGCAGCAGA ATGGAAGATG ATTGATTCTG AAGCTGTTGG AGACAGCACC 
AGGCAACAAG CTAAGCAGAT TTGCTATGGA ATCATCTATG GAATAGGAGC AAAATCTTTG 
GGTGAGCAGA TGGGCATTGA TGACAACAAA GCTGCCAATT ACATCGAATC CTTCAAATCA 
AGATACACAG GTATTCAGAA ATTCTTGAAG GAGACAGTAA GTAACTGCAG AAGAGATGGG 
TTTGTGCAGA CCATACTGGG AAGACGGAGA TACCTGCCAT CTATCAGAGA TTCAAATCCC 
TACATTAAAG CTCACGCAGA GCGCCAGGCT GTGAACACAA CTGTGCAGGG ATCTGCTGCT 
GACATTGTCA AAACAGCCAC TGTGAACATT CAGAGACGCC TGGAAGCTCT CTCTTCTGTG 
AACAAGTCCC ATGGACATCT GGAGAACTCA TTCCAGAGAG ATAAGACAGG AAGGCTGTCA 
AGGAAGAGAA ACAGAGAGAT GTTGCATCCC ATCAGTGGAG GTTTCTTTAT CCTTCAGCTC 
CATGATGAGC TCCTCTATGA AGTTGCAGAA GATGATGTGA TCCAGGTCGC TCAGATTGTC 
AAGCATGAGA TGGAAAATGC TGTCAAACTC TCAGTGAAAC TGAATGTTAA AGTGAAAATT 
GGACCTAGCT GGGGAGAGCT GCAGGACCTG GAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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POLQ cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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