POLQ cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLQ
Entrez Gene ID	566079
Full Name	polymerase (DNA directed), theta
Synonyms	bZ1P14.5,si:rp71-1p14.5
General protein information	Preferred Names polymerase (DNA directed), theta Names DNA polymerase theta
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009295407.3	XP_009293682.1	DNA polymerase theta
XM_009295407.2	XP_009293682.1	DNA polymerase theta isoform X1
XM_021473431.1	XP_021329106.1	DNA polymerase theta
XM_017352975.1	XP_017208464.1	DNA polymerase theta isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POLQ	XP_545125.3
Gallus gallus (chicken)	POLQ	XP_416549.3
Xenopus tropicalis (tropical clawed frog)	polq	NP_001184156.1
Neurospora crassa	NCU07411	XP_958186.2
Bos taurus (cattle)	POLQ	XP_002684835.1
Rattus norvegicus (Norway rat)	Polq	NP_001099348.1
Danio rerio (zebrafish)	si:rp71-1p14.5	XP_694437.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007533	XP_564726.3
Magnaporthe oryzae (rice blast fungus)	MGG_15295	XP_003711003.1
Homo sapiens (human)	POLQ	NP_955452.3
Macaca mulatta (Rhesus monkey)	POLQ	XP_001111254.2
Drosophila melanogaster (fruit fly)	mus308	NP_524333.1
Pan troglodytes (chimpanzee)	POLQ	XP_001165150.2
Mus musculus (house mouse)	Polq	NP_084253.1

Gene Ontology

The following POLQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146122	XM_021473431.1 Latest version!	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa44787	XM_009295407.3 Latest version!	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa56928	XM_017352975.1 Latest version!	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa44787	XM_009295407.2	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLQ ( XM_021473431.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329106.1 Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

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CloneID	ODa146122
Clone ID Related Accession (Same CDS sequence)	XM_021473431.1
Accession Version	XM_021473431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA polymerase theta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTT CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CTGTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCT TGAAGTTTAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAATTCCT GAAATTCAAA AAGAGCAAAA CACAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTAGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTGA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTGCAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT TTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC GGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GCTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCACTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACAA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACATATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTGTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TTCGGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAGC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAACTGTGAA ATCAAACACA 
GAACCACGGT CTATGTCAGG AGGCACAGAA CATGAAGGGG ATCAAGATTT AATGTCAAAT 
GTGACTGAAG AGAAATTACT GCTGGAAAAT AATTCTGACA ATAATTCACA CCCCCCTTTG 
AGGCCATTAG AATCCACTAC CAATTCAGAA AAAACCATGG ACGTTGACTC GGGTGTATCA 
TCTGCTGAAT GCCAACCTGA TAAGAAATGT GAACTGCAAT CAAGTGTACT TCATAAAGTT 
CTCAAATCTA TAAATACAAA AGACAAGGGC TGTGATAATA ACCACACAGT TGAATCATCA 
TCTGAAACAC TTCAGAGGCG AAATTCATGT CTTAATACCT GCATCTCTGG CAATGACCAA 
CACTGTGTTA GTCCAGTTTC AAAACGCAGA AGGATGGATG CTGCAGGTGA TGAAGAGGGA 
CCCACAGTCA CTGGAGTGAA GATGAGAATT GCAAATGGAC TTGCAACAGA CCGTAAGGAA 
ATGCCAAAAC CTGAAGAGCT TTGTAGAATA GATCAAGTCA AGATGAAACT GAACAAAGAC 
ACAGGGTTAA AAAAAGACTC TCGCTCATGT ACTCCACTCC CAAATGGGGA AGTCACAAAT 
GTCTGTGTGT TTTCCTCTGG TAAGGGGAAA AAGCGCACAT CAAAAATGAA ACATTTAAAT 
ATAAATGAAG ACATTTATCA TGAAACCCAA ACTGAAAAAG AAGAAATGGT TGCAGAAATT 
TATGGGAAAT TTAGCAACAT AGATCAGAAC GAAGGGGACT TGCATGTGAA CACCAGAGTA 
AAGAATGATT TAGTATGTAA TTCTGAGAAG CTTAGCTCTC CTGACTTGTA CACAAATGGA 
ATAGAAGAGT TTGGTGATAG TTTTCAGCTT GATACACAGA CTGAGAAAAT GCTTCACGGG 
CATGATTTTT CACATAGGAA TGTTGAATTT AACGTCAGAG AAAATAAACA GGATAACCAA 
GTTGATTCTA CAAGTGAGAA AGTTGTCAAA CAGTCATCTT TCCTGGAAAA GGAGAATCGA 
GTATCTAAAG AGCAAAACAA AGGCCCATTT GAAAGTTTAT CTCTCCATGA TAATGGCTGG 
CCCACCGAAG CCAAACCAAA ATACAACATT TCACTTACTG ACAGCCAGTT AGAGAACATT 
TTAAATTACA GTAATCAAGT TCCTGAAGAA GAACAATGTG CGAATAAACC TGCTGAGCAA 
AGGGAAAATA ATGAGTCTTC AGATCATGTT ACTGACAACA GTTTCGATCG GAGCAGCAGC 
TTTCTGTTTG ACAGTCTCTA TGACAACTCC ATCTTGGATG CCATGGAGGA GGCAGCAATA 
GATCAAGACA ACAAGGGAGA TCAGGAGGCT GAAGTGAAAG TTAATTCAAA TGGCTCTTCT 
CCAGAACTCA TTCCAGAGAG GAGAGAGGCT TTGCCAACAG ATGACCAGGA AGCAATCCAA 
TGGGGGGAAT CATCTTTCAA CCTTTCAGAA TGGGGAGATT CACTAATCAT TGGTGAGCAG 
TACCTTGAAA AAATAAGCAA GGCTTGTGAT GGCCCAAGAT TGGGTGCAGA GAAGCCAAGT 
TATACAGATG ACATAGTGTC TGAACTGAAT GTGTCACAAA GCAATGAATA TCAGCAAAAA 
GTTGAAAGGC ATCGATTAAA TAGCTCATCG TTTCATATCA GTCCAGGAAT GCAGGACTTG 
TTTGATAAAT GGTCTGATCA GTTTTCAACT GTACCTGATG CAACAGCTCA CTCAAATTCA 
TTAGTGGAAC CAAATGCTGT TGTCCTTGAA GAAGCTGTTT CCCTAACAAA AACCAAAGAG 
ATTGAGAAGG AAGGTCCAGA AGTTTCAAGA TCTGAAGACT TTGTAACAGT CCAGGCTAAG 
AAAGTGACTC CTAGTGACCT TGTTCCTCCC ACACAAGTTT TAGAACCAGT CACACCACGA 
GTCAAAATGA CCACTTCTGC TATACAATCA CCTAAAAATG TTACAAAGCA AAGGTCTGAA 
CTAGATCCAA ATATCAAATC ATGTACACAG AGATATCATC AACCTTGTCT GAGGACAAAC 
TCAACATCAG TGTTAGGCGC ACCTCTGGCT GACGAGGGCT TTACGAGACT TTCTCAGTGT 
CCTTCACTTC CTGCTTCTAA CTCCTGCAGT CCAGAAACGT TTTCCATAAT TGATGTGGCA 
AGCGACAGAA GGCTTTTTGA AACATTTGTA AACGAATGGA AAACAAAAGA GAGATTTTCT 
CTTGCTGTTG CCTGTGAGAA GACAGACAGC ACCTCAGTGC AACCTGAGAC TGTCATAGGT 
GGAAAATTCA AAAAACCTAC TACCCCAATG AGGAACAAAA GAAAAGATGG CTTCCTTCTG 
AAGGGATATG AGGATCTTGT GGTTATTGGC ATATCTGTAA GCTGGGGAGC AAAAGATGCT 
TATTTTGTAT CTCTACAACA GGAGCTTGTA GACACAGATA TAAGTGCCAG TTTAGCGCCA 
CCGCCTCTTG ATGATACGCT TACGGTTGAA GAGCGGCTGA AGCAGATTCA GTCCTGCTTG 
CAAAAAGACT CAAGTGTCAC AGTCACCTAT GACTTCATTC ATCTTTACAA AATACTTCTG 
CTGGCATGTG AACTTGCCGT GCGTGGCACA TTTGAAGACC CAAAGATTGC ATGTTGGCTT 
TTGGATTCAA GCTCCAAAGA GCGTACACTC CACAACATGG TGACCAGTTT CGCAACTGAG 
GACCTCCCAT TGTTGGAGGG AATTAGTGCG GGGCAGGGTG TGCAGAGTTT AGGAATCTTT 
GGAGAAGCCA GTCAACCTGG ACGCTACAGA GCTGCTATTG AGTCTGTTCT GGTCTTCAGG 
GTCATGACCC AACTCAACTG TCTACTAGAA AAGGATGGTT TCTTAGATGT ATTCAAGAAA 
GTAGAGATGC CAACTCAGTA CTGCCTGGCA TTGTTAGAGC TGAATGGAAT TGGCTTTAGC 
ATTGCCGAAT GTGAGGCTCA AAAACATGTC ATGCAAGCCA AACTAAGTGC TCTTGAATCC 
CAAGCTTATC AGCTAGCAGG ACACAGCTTC TCATTGACCA GTCCAGAGGA TGTAGCAGAG 
GTGTTGTTTC TTGAGTTGAA GCTGCCTCCA AATGGAGATT TAAATGGTCT GAAGAATAAG 
AAGACGCTGG GCTACACTAG GAGAGCAGGA GCTCGTATAA AACTTTCTAA ACAGTTCAGC 
ACAACCAAGG ATGTTTTAGA GAAGCTCAAG CCATTACATC CTTTACCAGG TGTTATTCTT 
GAGTGGAGAA GAATCACAAA TGCGTTGACA AAAGTGGTTT TCCCCCTGCA ACGAGAGAAA 
AAGTGGCACT CACACCTGAA GATGGACAGA ATACATCCAA TCTCACAGTC GCACACTGCC 
ACAGGACGTG TGAGCTTCAC TGAACCAAAC ATTCAAAATG TGCCAAAAGA TTTTGAAATT 
CAGATGCCAA CACTTATAGA AGAAAGTCAA ACGTCACAGA ATGGTGGAAG CAAGATGTGG 
TGTAAACGAA CAAAGATAAA CCGACAGTTG GCACCACTTT TAAAAGTTTC AGACAAATCA 
CCAGACAAAG GGATGCAATT CTCTGTCAGC ATGAGGCATG CTTTTGTTCC CTTTTCAGGA 
GGTCTGATAT TGGCTGTTGA CTACTCACAA CTGGAGCTCC GCATCTTAGC TCATCTCTCC 
CGGGACAGAA GACTCCTCCA TGTGCTCAAC AGTGGTGCAG ATGTGTTTAA AAGCATAGCA 
GCTGAGTGGA AAATGGTGGA CCCTGCTTCA GTTGATGATA ACATGAGGCA GCAGGCCAAA 
CAGATTTGTT ATGGAATAAT CTACGGTATG GGTGCAAAGT CACTCGGAGA GCAAATGGGC 
ATTGAGGAGA ATGATGCTGC CTGTTACATT GAGACATTTA AATCCAGATA CAATGGCATA 
CAGAACTTTC TGCGTGAAAC TGTGCAAAAG TGTGGGAAAA ACGGTTATGT AAAGACATTA 
CTCGGCAGGA AGCGTTTCTT GCCTGGAATT AAAGACTCAA ATGTGTACAT CAAGTCTCAC 
GCAGAGAGAC AGGCAGTAAA CACAACTGTT CAGGGCTCTG CTGCTGACAT TGTGAAATTA 
GCAACCATCA ACATCCAGCG CAGAATAGAA GAAGCGTTTC CTGGAGTGCC CACATCACAC 
CAACACCCTT CAATCAGATT GGGTGGTCGA CACCGAAATC AGTTTAGACC CCTTCGAGGA 
GGCTACTTTA TACTTCAACT GCATGATGAA CTTATCTACG AGGTCGCAGA GGAAGATGTC 
ATTCAGGTTG CACAAATTGT GAAGCGAGAA ATGGAGTCTG TTGTTAAATT ATACGTGAAG 
CTCAGAGTAA AAGTTAAAGT AGGGCCAAGC TGGGGTAACC TTCAAGACCT GGACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329106.1
CDS	359..8098
Translation	MMTSTTRKIYLGQHRIVKTRGLSLADEKPGQRQLSNLCQEDVSENRSCIAPKQIQSKQWMPGDSSLAMDEEILQAIDELGPEVLKFKKIEKIQQTEPAPLKKNCDFPKIPEIQKEQNTENISPSSPSEHKRTRTQAKKEGDWKDLAQKLLFSETKKIEVGGRTHDLKQNRDYSSPQKILNSMKKTSKVAKSKQKLNFREVTSPNDSKDYILFSPTHMAAAKERSVLQQQKRSLKNQSVSVLTPPPGLDLTVLGDATLSDAAQSIHMGRPADQLDKLLLSSWGLPKPVLEKYQSLGVHRMFEWQAECLTLGKVLEGQNLVYSAPTSAGKTLVSELLILKRVLETRQKAMFILPFVSVAREKMFYLQNVFQEAGIRVEGYMGSTSAAGGFSALDVAVCTIEKANGLVNRLIEEDKLDLLGTVVVDELHMVGDSGRGYLLELLLTKIRYIAQKTATRNSGQSTSTGVQIIGMSATLPNLDLLARWLSAELYSTNYRPVPLMEWVKIGTNIYDGSLNLVRQFTPALPIKGDDDHIVSLCFETVQDGHSALLFCPSKNWCEKLADSIGREFYNLHHKEMQSGSGGQSIFLNQEGLLDVLAQLKRTPAGLDHVLQRTVPWGVAFHHAGLTFDERDILEGAFRQGYIRVLAATSTLSSGVNLPARRVIIRTPVFNGHLLDILTYKQMVGRAGRKGVDTIGESVLVCKEAERAKGMSLIQGSLKPISSCLVKREGEGVTTSMLRAILEIIVGGVASSPQDVRMYAACTLLAASIAAEEAHQDGSESEARNKGAIEACIEWLMDNEFIHIQKEGDVERYCPTHLGSATLSSSLSPPEALGIFADLQRAMKGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSAMKRVAEMVGIQEGFLARSVGGKLIAKTEKQRRQMAIHKRFFTTLVLLDLVSEEPLGAVAKKYGCSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLELLLSQFQSRLSFGVQRELCDLVRISLLTAQRARTLYSSGFVTVAELARADVSEVEKALRKAIPFKSSRQAVDESEVEAQERKSMRCIWVSGKKALTEREAAQQIVAEAQLLLQKDLALLGVEWSPACLPAKTQPDNSSTVKSNTEPRSMSGGTEHEGDQDLMSNVTEEKLLLENNSDNNSHPPLRPLESTTNSEKTMDVDSGVSSAECQPDKKCELQSSVLHKVLKSINTKDKGCDNNHTVESSSETLQRRNSCLNTCISGNDQHCVSPVSKRRRMDAAGDEEGPTVTGVKMRIANGLATDRKEMPKPEELCRIDQVKMKLNKDTGLKKDSRSCTPLPNGEVTNVCVFSSGKGKKRTSKMKHLNINEDIYHETQTEKEEMVAEIYGKFSNIDQNEGDLHVNTRVKNDLVCNSEKLSSPDLYTNGIEEFGDSFQLDTQTEKMLHGHDFSHRNVEFNVRENKQDNQVDSTSEKVVKQSSFLEKENRVSKEQNKGPFESLSLHDNGWPTEAKPKYNISLTDSQLENILNYSNQVPEEEQCANKPAEQRENNESSDHVTDNSFDRSSSFLFDSLYDNSILDAMEEAAIDQDNKGDQEAEVKVNSNGSSPELIPERREALPTDDQEAIQWGESSFNLSEWGDSLIIGEQYLEKISKACDGPRLGAEKPSYTDDIVSELNVSQSNEYQQKVERHRLNSSSFHISPGMQDLFDKWSDQFSTVPDATAHSNSLVEPNAVVLEEAVSLTKTKEIEKEGPEVSRSEDFVTVQAKKVTPSDLVPPTQVLEPVTPRVKMTTSAIQSPKNVTKQRSELDPNIKSCTQRYHQPCLRTNSTSVLGAPLADEGFTRLSQCPSLPASNSCSPETFSIIDVASDRRLFETFVNEWKTKERFSLAVACEKTDSTSVQPETVIGGKFKKPTTPMRNKRKDGFLLKGYEDLVVIGISVSWGAKDAYFVSLQQELVDTDISASLAPPPLDDTLTVEERLKQIQSCLQKDSSVTVTYDFIHLYKILLLACELAVRGTFEDPKIACWLLDSSSKERTLHNMVTSFATEDLPLLEGISAGQGVQSLGIFGEASQPGRYRAAIESVLVFRVMTQLNCLLEKDGFLDVFKKVEMPTQYCLALLELNGIGFSIAECEAQKHVMQAKLSALESQAYQLAGHSFSLTSPEDVAEVLFLELKLPPNGDLNGLKNKKTLGYTRRAGARIKLSKQFSTTKDVLEKLKPLHPLPGVILEWRRITNALTKVVFPLQREKKWHSHLKMDRIHPISQSHTATGRVSFTEPNIQNVPKDFEIQMPTLIEESQTSQNGGSKMWCKRTKINRQLAPLLKVSDKSPDKGMQFSVSMRHAFVPFSGGLILAVDYSQLELRILAHLSRDRRLLHVLNSGADVFKSIAAEWKMVDPASVDDNMRQQAKQICYGIIYGMGAKSLGEQMGIEENDAACYIETFKSRYNGIQNFLRETVQKCGKNGYVKTLLGRKRFLPGIKDSNVYIKSHAERQAVNTTVQGSAADIVKLATINIQRRIEEAFPGVPTSHQHPSIRLGGRHRNQFRPLRGGYFILQLHDELIYEVAEEDVIQVAQIVKREMESVVKLYVKLRVKVKVGPSWGNLQDLDI

Target ORF information:

RefSeq Version	XM_021473431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473431.1

ORF Insert Sequence:

ATGATGACTT CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CTGTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCT TGAAGTTTAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAATTCCT GAAATTCAAA AAGAGCAAAA CACAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTAGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTGA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTGCAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT TTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC GGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GCTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCACTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACAA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACATATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTGTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TTCGGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAGC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAACTGTGAA ATCAAACACA 
GAACCACGGT CTATGTCAGG AGGCACAGAA CATGAAGGGG ATCAAGATTT AATGTCAAAT 
GTGACTGAAG AGAAATTACT GCTGGAAAAT AATTCTGACA ATAATTCACA CCCCCCTTTG 
AGGCCATTAG AATCCACTAC CAATTCAGAA AAAACCATGG ACGTTGACTC GGGTGTATCA 
TCTGCTGAAT GCCAACCTGA TAAGAAATGT GAACTGCAAT CAAGTGTACT TCATAAAGTT 
CTCAAATCTA TAAATACAAA AGACAAGGGC TGTGATAATA ACCACACAGT TGAATCATCA 
TCTGAAACAC TTCAGAGGCG AAATTCATGT CTTAATACCT GCATCTCTGG CAATGACCAA 
CACTGTGTTA GTCCAGTTTC AAAACGCAGA AGGATGGATG CTGCAGGTGA TGAAGAGGGA 
CCCACAGTCA CTGGAGTGAA GATGAGAATT GCAAATGGAC TTGCAACAGA CCGTAAGGAA 
ATGCCAAAAC CTGAAGAGCT TTGTAGAATA GATCAAGTCA AGATGAAACT GAACAAAGAC 
ACAGGGTTAA AAAAAGACTC TCGCTCATGT ACTCCACTCC CAAATGGGGA AGTCACAAAT 
GTCTGTGTGT TTTCCTCTGG TAAGGGGAAA AAGCGCACAT CAAAAATGAA ACATTTAAAT 
ATAAATGAAG ACATTTATCA TGAAACCCAA ACTGAAAAAG AAGAAATGGT TGCAGAAATT 
TATGGGAAAT TTAGCAACAT AGATCAGAAC GAAGGGGACT TGCATGTGAA CACCAGAGTA 
AAGAATGATT TAGTATGTAA TTCTGAGAAG CTTAGCTCTC CTGACTTGTA CACAAATGGA 
ATAGAAGAGT TTGGTGATAG TTTTCAGCTT GATACACAGA CTGAGAAAAT GCTTCACGGG 
CATGATTTTT CACATAGGAA TGTTGAATTT AACGTCAGAG AAAATAAACA GGATAACCAA 
GTTGATTCTA CAAGTGAGAA AGTTGTCAAA CAGTCATCTT TCCTGGAAAA GGAGAATCGA 
GTATCTAAAG AGCAAAACAA AGGCCCATTT GAAAGTTTAT CTCTCCATGA TAATGGCTGG 
CCCACCGAAG CCAAACCAAA ATACAACATT TCACTTACTG ACAGCCAGTT AGAGAACATT 
TTAAATTACA GTAATCAAGT TCCTGAAGAA GAACAATGTG CGAATAAACC TGCTGAGCAA 
AGGGAAAATA ATGAGTCTTC AGATCATGTT ACTGACAACA GTTTCGATCG GAGCAGCAGC 
TTTCTGTTTG ACAGTCTCTA TGACAACTCC ATCTTGGATG CCATGGAGGA GGCAGCAATA 
GATCAAGACA ACAAGGGAGA TCAGGAGGCT GAAGTGAAAG TTAATTCAAA TGGCTCTTCT 
CCAGAACTCA TTCCAGAGAG GAGAGAGGCT TTGCCAACAG ATGACCAGGA AGCAATCCAA 
TGGGGGGAAT CATCTTTCAA CCTTTCAGAA TGGGGAGATT CACTAATCAT TGGTGAGCAG 
TACCTTGAAA AAATAAGCAA GGCTTGTGAT GGCCCAAGAT TGGGTGCAGA GAAGCCAAGT 
TATACAGATG ACATAGTGTC TGAACTGAAT GTGTCACAAA GCAATGAATA TCAGCAAAAA 
GTTGAAAGGC ATCGATTAAA TAGCTCATCG TTTCATATCA GTCCAGGAAT GCAGGACTTG 
TTTGATAAAT GGTCTGATCA GTTTTCAACT GTACCTGATG CAACAGCTCA CTCAAATTCA 
TTAGTGGAAC CAAATGCTGT TGTCCTTGAA GAAGCTGTTT CCCTAACAAA AACCAAAGAG 
ATTGAGAAGG AAGGTCCAGA AGTTTCAAGA TCTGAAGACT TTGTAACAGT CCAGGCTAAG 
AAAGTGACTC CTAGTGACCT TGTTCCTCCC ACACAAGTTT TAGAACCAGT CACACCACGA 
GTCAAAATGA CCACTTCTGC TATACAATCA CCTAAAAATG TTACAAAGCA AAGGTCTGAA 
CTAGATCCAA ATATCAAATC ATGTACACAG AGATATCATC AACCTTGTCT GAGGACAAAC 
TCAACATCAG TGTTAGGCGC ACCTCTGGCT GACGAGGGCT TTACGAGACT TTCTCAGTGT 
CCTTCACTTC CTGCTTCTAA CTCCTGCAGT CCAGAAACGT TTTCCATAAT TGATGTGGCA 
AGCGACAGAA GGCTTTTTGA AACATTTGTA AACGAATGGA AAACAAAAGA GAGATTTTCT 
CTTGCTGTTG CCTGTGAGAA GACAGACAGC ACCTCAGTGC AACCTGAGAC TGTCATAGGT 
GGAAAATTCA AAAAACCTAC TACCCCAATG AGGAACAAAA GAAAAGATGG CTTCCTTCTG 
AAGGGATATG AGGATCTTGT GGTTATTGGC ATATCTGTAA GCTGGGGAGC AAAAGATGCT 
TATTTTGTAT CTCTACAACA GGAGCTTGTA GACACAGATA TAAGTGCCAG TTTAGCGCCA 
CCGCCTCTTG ATGATACGCT TACGGTTGAA GAGCGGCTGA AGCAGATTCA GTCCTGCTTG 
CAAAAAGACT CAAGTGTCAC AGTCACCTAT GACTTCATTC ATCTTTACAA AATACTTCTG 
CTGGCATGTG AACTTGCCGT GCGTGGCACA TTTGAAGACC CAAAGATTGC ATGTTGGCTT 
TTGGATTCAA GCTCCAAAGA GCGTACACTC CACAACATGG TGACCAGTTT CGCAACTGAG 
GACCTCCCAT TGTTGGAGGG AATTAGTGCG GGGCAGGGTG TGCAGAGTTT AGGAATCTTT 
GGAGAAGCCA GTCAACCTGG ACGCTACAGA GCTGCTATTG AGTCTGTTCT GGTCTTCAGG 
GTCATGACCC AACTCAACTG TCTACTAGAA AAGGATGGTT TCTTAGATGT ATTCAAGAAA 
GTAGAGATGC CAACTCAGTA CTGCCTGGCA TTGTTAGAGC TGAATGGAAT TGGCTTTAGC 
ATTGCCGAAT GTGAGGCTCA AAAACATGTC ATGCAAGCCA AACTAAGTGC TCTTGAATCC 
CAAGCTTATC AGCTAGCAGG ACACAGCTTC TCATTGACCA GTCCAGAGGA TGTAGCAGAG 
GTGTTGTTTC TTGAGTTGAA GCTGCCTCCA AATGGAGATT TAAATGGTCT GAAGAATAAG 
AAGACGCTGG GCTACACTAG GAGAGCAGGA GCTCGTATAA AACTTTCTAA ACAGTTCAGC 
ACAACCAAGG ATGTTTTAGA GAAGCTCAAG CCATTACATC CTTTACCAGG TGTTATTCTT 
GAGTGGAGAA GAATCACAAA TGCGTTGACA AAAGTGGTTT TCCCCCTGCA ACGAGAGAAA 
AAGTGGCACT CACACCTGAA GATGGACAGA ATACATCCAA TCTCACAGTC GCACACTGCC 
ACAGGACGTG TGAGCTTCAC TGAACCAAAC ATTCAAAATG TGCCAAAAGA TTTTGAAATT 
CAGATGCCAA CACTTATAGA AGAAAGTCAA ACGTCACAGA ATGGTGGAAG CAAGATGTGG 
TGTAAACGAA CAAAGATAAA CCGACAGTTG GCACCACTTT TAAAAGTTTC AGACAAATCA 
CCAGACAAAG GGATGCAATT CTCTGTCAGC ATGAGGCATG CTTTTGTTCC CTTTTCAGGA 
GGTCTGATAT TGGCTGTTGA CTACTCACAA CTGGAGCTCC GCATCTTAGC TCATCTCTCC 
CGGGACAGAA GACTCCTCCA TGTGCTCAAC AGTGGTGCAG ATGTGTTTAA AAGCATAGCA 
GCTGAGTGGA AAATGGTGGA CCCTGCTTCA GTTGATGATA ACATGAGGCA GCAGGCCAAA 
CAGATTTGTT ATGGAATAAT CTACGGTATG GGTGCAAAGT CACTCGGAGA GCAAATGGGC 
ATTGAGGAGA ATGATGCTGC CTGTTACATT GAGACATTTA AATCCAGATA CAATGGCATA 
CAGAACTTTC TGCGTGAAAC TGTGCAAAAG TGTGGGAAAA ACGGTTATGT AAAGACATTA 
CTCGGCAGGA AGCGTTTCTT GCCTGGAATT AAAGACTCAA ATGTGTACAT CAAGTCTCAC 
GCAGAGAGAC AGGCAGTAAA CACAACTGTT CAGGGCTCTG CTGCTGACAT TGTGAAATTA 
GCAACCATCA ACATCCAGCG CAGAATAGAA GAAGCGTTTC CTGGAGTGCC CACATCACAC 
CAACACCCTT CAATCAGATT GGGTGGTCGA CACCGAAATC AGTTTAGACC CCTTCGAGGA 
GGCTACTTTA TACTTCAACT GCATGATGAA CTTATCTACG AGGTCGCAGA GGAAGATGTC 
ATTCAGGTTG CACAAATTGT GAAGCGAGAA ATGGAGTCTG TTGTTAAATT ATACGTGAAG 
CTCAGAGTAA AAGTTAAAGT AGGGCCAAGC TGGGGTAACC TTCAAGACCT GGACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_009295407.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293682.1 Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

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CloneID	ODa44787
Clone ID Related Accession (Same CDS sequence)	XM_009295407.3 , XM_009295407.2
Accession Version	XM_009295407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA polymerase theta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295407.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GATGTTTTAG AGAAGCTCAA GCCATTACAT CCTTTACCAG GTGTTATTCT TGAGTGGAGA 
AGAATCACAA ATGCGTTGAC AAAAGTGGTT TTCCCCCTGC AACGAGAGAA AAAGTGGCAC 
TCACACCTGA AGATGGACAG AATACATCCA ATCTCACAGT CGCACACTGC CACAGGACGT 
GTGAGCTTCA CTGAACCAAA CATTCAAAAT GTGCCAAAAG ATTTTGAAAT TCAGATGCCA 
ACACTTATAG AAGAAAGTCA AACGTCACAG AATGGTGGAA GCAAGATGTG GTGTAAACGA 
ACAAAGATAA ACCGACTGTT GGCACCACTT TTAAAAGTTT CAGACAAATC ACCAGACAAA 
GGGATGCAAT TCTCTGTCAG CATGAGGCAT GCTTTTGTTC CCTTTTCAGG AGGTCTGATA 
TTAGCTGTTG ACTACTCACA ACTGGAGCTC CGCATCTTAG CTCATCTCTC CCGGGACAGA 
AGACTCCTCC ATGTGCTCAA CAGTGGTGCA GATGTGTTTA AAAGCATAGC AGCTGAGTGG 
AAAATGGTGG ACCCTGCTTC AGTTGATGAT AACATGAGGC AGCAGGCCAA ACAGATTTGT 
TATGGAATAA TCTACGGTAT GGGTGCAAAG TCACTCGGAG AGCAAATGGG CATTGAGGAG 
AATGATGCTG CCTGTTACAT TGAGACATTT AAATCCAGAT ACAATGGCAT ACAGAACTTT 
CTGCGTGAAA CTGTGCAAAA GTGTGGGAAA AACGGTTATG TAAAGACATT ACTCGGCAGG 
AAGCGTTTCT TGCCTGGAAT TAAAGACTCA AATGTGTACA TCAAGTCTCA CGCAGAGAGA 
CAGGCAGTAA ACACAACTGT TCAGGGCTCT GCTGCTGACA TTGTGAAATT AGCAACCATC 
AACATCCAGC GCAGAATAGA AGAAGCGTTT CCTGGAGTGC CCACATCACA CCAACACCCT 
TCAATCAGAT TGGGTGGTCG ACACCGAAAT CAGTTTAGAC CCCTTCGAGG AGGCTACTTT 
ATACTTCAAC TGCATGATGA ACTCATCTAC GAGGTCGCAG AGGAAGATGT CATTCAGGTT 
GCACAAATTG TGAAGCGAGA AATGGAGTCT GTTGTTAAAT TATACGTGAA GCTCAGAGTA 
AAAGTTAAAG TAGGGCCAAG CTGGGGTAAC CTTCAAGACC TGGACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293682.1
CDS	339..8069
Translation	MMTATTRKIYLGQHRIVKTRGLSLADEKPGQRQLSNLCQEDVSENRSIIAPKQIQSKQWMPGDSSLAMDEEILQAIDELGPEVMKFKKIEKIQQTEPAPLKKNCDFPKNAEIPKEQNAENISPSSPSEHKRTRTQAKKEGDWKDLAQKLLFSETKKIEVGGRTHDLKQNRDYSSPQKILNSMKKTSKVAKSKQKLNFREVTSPNDSKDYILFSPTHMAAAKERSVLQQQKRSLKNQSVSVLTPPPGLDLTVLGDATLSDAAQSIHMGRPADQLDKLLLSSWGLPKPVLEKYQSLGVHRMFEWQAECLTLGKVLEGQNLVYSAPTSAGKTLVSELLILKRVLETRQKAMFILPFVSVAREKMFYLQNVFQEAGIRVEGYMGSTSAAGGFSALDVAVCTIEKANGLVNRLIEEDKLDLLGTVVVDELHMVGDSGRGYLLELLLTKIRYIAQKTATRNSGQSTSTGVQIIGMSATLPNLDLLARWLSAELYSTNYRPVPLMEWVKIGTNIYDGSLNLVRQFTPALPIKGDDDHIVSLCFETVQDGHSALLFCPSKNWCEKLADSIGREFYNLHHKEMQSGSGGQSISLNQEGLLDVLAQLKRTPAGLDHVLQRTVPWGVAFHHAGLTFDERDILEGAFRQGYIRVLAATSTLSSGVNLPARRVIIRTPVFNGHLLDILTYKQMVGRAGRKGVDTIGESVLVCKEAERAKGMSLIQGSLKPISSCLVKREGEGVTTSMLRAILEIIVGGVASSPQDVRMYAACTLLAASIAAEEVHQDGSESEARNKGAIEACIEWLMDNEFIHIQKEGDVERYCPTHLGSATLSSSLSPPEALGIFADLQRAMKGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSAMKRVAEMVGIQEGFLARSVGGKLIAKTEKQRRQMAIHKRFFTTLVLLDLVSEEPLGAVAKKYGCSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLELLLSQFQSRLSFGVQRELCDLVRISLLTAQRARTLYSSGFVTVAELARADVTEVEKALRKAIPFKSSRQAVDESEVEAQERKSMRCIWVSGKKALTEREAAQQIVAEAQLLLQKDLALLGVEWNPACLPAKTQPDNSSNTEPRSMSGGTEHEGGQDLMLNVTEEKLLLENNSNNNSHPPLRPLESTTNLEKTMDVDSGVSSAECQPDKKCELQSSVLHKVLKSINAKDKGCDNNQTVESSSESLQRRNSCLNTCISGNDQHCVSPVSKRRRMDAAGDEEGPTVTGVKMRIANGLATDRKEMPKPEELCRIDQVKMKLNKDTGLKKDSRSCTPLPNEEVTNVCVVSSGKGKKRTSKMKHLNINADINHETQTEKEEMVAEIYGKFSNRDQNEGDLHVNTRVKNDLVCNSEKLSSPDLYTNGLEEFGDSFQLDTQTEKMLHGHDFSHRNVELNVVRENKQDNQVDSTSEKVVKQSSFLEKENRVSKEQNKGPFESLSLHDNGWPTEAKPKYNISLTDSQLENILNYSNQVPEEEQCANKPAEQRENNESSDHVTDNSFNRSSSFLFDSLYDNSILDAMEEAAIDQDNKGDQEAEVKVNSNGSSPELIPERREALPTDDQEAIQWGESSFNLSEWGDSLIIGEQYLEKISKACDGPRLGAEKPSYTDDIVSELNVSQSNEYQQKVERHRLNSSSFHISPGMQDLFDKWSDQFSTVPDATAHSNSLVEPNAVVLEEAVSLTKTKEIEKEGPEVSRSEDFVTVQAKKVTPSDLVPPTQVLEPVTPRVKMTTSAIQSPKNVTKQRSELDPNIKSCTQRYHQPCLRTNSTSVLGAPLADEGLTRLSQCPSLPASNSCSPETFSIIDVASDRRLFETFVNEWKTKERFSLAVACEKTDSTSVQPETVIGGKFKKPTTPMRNKRKDGFLLKGYEDLVVIGISVSWGAKDAYFVSLQQELVDTDISASLAPPPLDDTLTVEERLKQIQSCLQKDSSVTVTYDFIHLYKILLLACELAVRGTFEDPKIACWLLDSSSKERTLHNMVTSFATEDLPMLEGISAGQGVQSLGIYGEASQPGRYRAAIESVLVFRVMTQLNCLLEKDGFLDVFKKVEMPTQYCLALLELNGIGFSIAECEAQKHVMQAKLSALESQAYQLAGHSFSLTSPEDVAEVLFLELKLPPNGDLNGLKNKKTLGYTRRAGARIKLSKQFSTTKDVLEKLKPLHPLPGVILEWRRITNALTKVVFPLQREKKWHSHLKMDRIHPISQSHTATGRVSFTEPNIQNVPKDFEIQMPTLIEESQTSQNGGSKMWCKRTKINRLLAPLLKVSDKSPDKGMQFSVSMRHAFVPFSGGLILAVDYSQLELRILAHLSRDRRLLHVLNSGADVFKSIAAEWKMVDPASVDDNMRQQAKQICYGIIYGMGAKSLGEQMGIEENDAACYIETFKSRYNGIQNFLRETVQKCGKNGYVKTLLGRKRFLPGIKDSNVYIKSHAERQAVNTTVQGSAADIVKLATINIQRRIEEAFPGVPTSHQHPSIRLGGRHRNQFRPLRGGYFILQLHDELIYEVAEEDVIQVAQIVKREMESVVKLYVKLRVKVKVGPSWGNLQDLDI

Target ORF information:

RefSeq Version	XM_009295407.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295407.3

ORF Insert Sequence:

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GATGTTTTAG AGAAGCTCAA GCCATTACAT CCTTTACCAG GTGTTATTCT TGAGTGGAGA 
AGAATCACAA ATGCGTTGAC AAAAGTGGTT TTCCCCCTGC AACGAGAGAA AAAGTGGCAC 
TCACACCTGA AGATGGACAG AATACATCCA ATCTCACAGT CGCACACTGC CACAGGACGT 
GTGAGCTTCA CTGAACCAAA CATTCAAAAT GTGCCAAAAG ATTTTGAAAT TCAGATGCCA 
ACACTTATAG AAGAAAGTCA AACGTCACAG AATGGTGGAA GCAAGATGTG GTGTAAACGA 
ACAAAGATAA ACCGACTGTT GGCACCACTT TTAAAAGTTT CAGACAAATC ACCAGACAAA 
GGGATGCAAT TCTCTGTCAG CATGAGGCAT GCTTTTGTTC CCTTTTCAGG AGGTCTGATA 
TTAGCTGTTG ACTACTCACA ACTGGAGCTC CGCATCTTAG CTCATCTCTC CCGGGACAGA 
AGACTCCTCC ATGTGCTCAA CAGTGGTGCA GATGTGTTTA AAAGCATAGC AGCTGAGTGG 
AAAATGGTGG ACCCTGCTTC AGTTGATGAT AACATGAGGC AGCAGGCCAA ACAGATTTGT 
TATGGAATAA TCTACGGTAT GGGTGCAAAG TCACTCGGAG AGCAAATGGG CATTGAGGAG 
AATGATGCTG CCTGTTACAT TGAGACATTT AAATCCAGAT ACAATGGCAT ACAGAACTTT 
CTGCGTGAAA CTGTGCAAAA GTGTGGGAAA AACGGTTATG TAAAGACATT ACTCGGCAGG 
AAGCGTTTCT TGCCTGGAAT TAAAGACTCA AATGTGTACA TCAAGTCTCA CGCAGAGAGA 
CAGGCAGTAA ACACAACTGT TCAGGGCTCT GCTGCTGACA TTGTGAAATT AGCAACCATC 
AACATCCAGC GCAGAATAGA AGAAGCGTTT CCTGGAGTGC CCACATCACA CCAACACCCT 
TCAATCAGAT TGGGTGGTCG ACACCGAAAT CAGTTTAGAC CCCTTCGAGG AGGCTACTTT 
ATACTTCAAC TGCATGATGA ACTCATCTAC GAGGTCGCAG AGGAAGATGT CATTCAGGTT 
GCACAAATTG TGAAGCGAGA AATGGAGTCT GTTGTTAAAT TATACGTGAA GCTCAGAGTA 
AAAGTTAAAG TAGGGCCAAG CTGGGGTAAC CTTCAAGACC TGGACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_009295407.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293682.1 Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

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CloneID	ODa44787
Clone ID Related Accession (Same CDS sequence)	XM_009295407.3 , XM_009295407.2
Accession Version	XM_009295407.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase theta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295407.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GATGTTTTAG AGAAGCTCAA GCCATTACAT CCTTTACCAG GTGTTATTCT TGAGTGGAGA 
AGAATCACAA ATGCGTTGAC AAAAGTGGTT TTCCCCCTGC AACGAGAGAA AAAGTGGCAC 
TCACACCTGA AGATGGACAG AATACATCCA ATCTCACAGT CGCACACTGC CACAGGACGT 
GTGAGCTTCA CTGAACCAAA CATTCAAAAT GTGCCAAAAG ATTTTGAAAT TCAGATGCCA 
ACACTTATAG AAGAAAGTCA AACGTCACAG AATGGTGGAA GCAAGATGTG GTGTAAACGA 
ACAAAGATAA ACCGACTGTT GGCACCACTT TTAAAAGTTT CAGACAAATC ACCAGACAAA 
GGGATGCAAT TCTCTGTCAG CATGAGGCAT GCTTTTGTTC CCTTTTCAGG AGGTCTGATA 
TTAGCTGTTG ACTACTCACA ACTGGAGCTC CGCATCTTAG CTCATCTCTC CCGGGACAGA 
AGACTCCTCC ATGTGCTCAA CAGTGGTGCA GATGTGTTTA AAAGCATAGC AGCTGAGTGG 
AAAATGGTGG ACCCTGCTTC AGTTGATGAT AACATGAGGC AGCAGGCCAA ACAGATTTGT 
TATGGAATAA TCTACGGTAT GGGTGCAAAG TCACTCGGAG AGCAAATGGG CATTGAGGAG 
AATGATGCTG CCTGTTACAT TGAGACATTT AAATCCAGAT ACAATGGCAT ACAGAACTTT 
CTGCGTGAAA CTGTGCAAAA GTGTGGGAAA AACGGTTATG TAAAGACATT ACTCGGCAGG 
AAGCGTTTCT TGCCTGGAAT TAAAGACTCA AATGTGTACA TCAAGTCTCA CGCAGAGAGA 
CAGGCAGTAA ACACAACTGT TCAGGGCTCT GCTGCTGACA TTGTGAAATT AGCAACCATC 
AACATCCAGC GCAGAATAGA AGAAGCGTTT CCTGGAGTGC CCACATCACA CCAACACCCT 
TCAATCAGAT TGGGTGGTCG ACACCGAAAT CAGTTTAGAC CCCTTCGAGG AGGCTACTTT 
ATACTTCAAC TGCATGATGA ACTCATCTAC GAGGTCGCAG AGGAAGATGT CATTCAGGTT 
GCACAAATTG TGAAGCGAGA AATGGAGTCT GTTGTTAAAT TATACGTGAA GCTCAGAGTA 
AAAGTTAAAG TAGGGCCAAG CTGGGGTAAC CTTCAAGACC TGGACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293682.1
CDS	326..8056
Translation	MMTATTRKIYLGQHRIVKTRGLSLADEKPGQRQLSNLCQEDVSENRSIIAPKQIQSKQWMPGDSSLAMDEEILQAIDELGPEVMKFKKIEKIQQTEPAPLKKNCDFPKNAEIPKEQNAENISPSSPSEHKRTRTQAKKEGDWKDLAQKLLFSETKKIEVGGRTHDLKQNRDYSSPQKILNSMKKTSKVAKSKQKLNFREVTSPNDSKDYILFSPTHMAAAKERSVLQQQKRSLKNQSVSVLTPPPGLDLTVLGDATLSDAAQSIHMGRPADQLDKLLLSSWGLPKPVLEKYQSLGVHRMFEWQAECLTLGKVLEGQNLVYSAPTSAGKTLVSELLILKRVLETRQKAMFILPFVSVAREKMFYLQNVFQEAGIRVEGYMGSTSAAGGFSALDVAVCTIEKANGLVNRLIEEDKLDLLGTVVVDELHMVGDSGRGYLLELLLTKIRYIAQKTATRNSGQSTSTGVQIIGMSATLPNLDLLARWLSAELYSTNYRPVPLMEWVKIGTNIYDGSLNLVRQFTPALPIKGDDDHIVSLCFETVQDGHSALLFCPSKNWCEKLADSIGREFYNLHHKEMQSGSGGQSISLNQEGLLDVLAQLKRTPAGLDHVLQRTVPWGVAFHHAGLTFDERDILEGAFRQGYIRVLAATSTLSSGVNLPARRVIIRTPVFNGHLLDILTYKQMVGRAGRKGVDTIGESVLVCKEAERAKGMSLIQGSLKPISSCLVKREGEGVTTSMLRAILEIIVGGVASSPQDVRMYAACTLLAASIAAEEVHQDGSESEARNKGAIEACIEWLMDNEFIHIQKEGDVERYCPTHLGSATLSSSLSPPEALGIFADLQRAMKGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSAMKRVAEMVGIQEGFLARSVGGKLIAKTEKQRRQMAIHKRFFTTLVLLDLVSEEPLGAVAKKYGCSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLELLLSQFQSRLSFGVQRELCDLVRISLLTAQRARTLYSSGFVTVAELARADVTEVEKALRKAIPFKSSRQAVDESEVEAQERKSMRCIWVSGKKALTEREAAQQIVAEAQLLLQKDLALLGVEWNPACLPAKTQPDNSSNTEPRSMSGGTEHEGGQDLMLNVTEEKLLLENNSNNNSHPPLRPLESTTNLEKTMDVDSGVSSAECQPDKKCELQSSVLHKVLKSINAKDKGCDNNQTVESSSESLQRRNSCLNTCISGNDQHCVSPVSKRRRMDAAGDEEGPTVTGVKMRIANGLATDRKEMPKPEELCRIDQVKMKLNKDTGLKKDSRSCTPLPNEEVTNVCVVSSGKGKKRTSKMKHLNINADINHETQTEKEEMVAEIYGKFSNRDQNEGDLHVNTRVKNDLVCNSEKLSSPDLYTNGLEEFGDSFQLDTQTEKMLHGHDFSHRNVELNVVRENKQDNQVDSTSEKVVKQSSFLEKENRVSKEQNKGPFESLSLHDNGWPTEAKPKYNISLTDSQLENILNYSNQVPEEEQCANKPAEQRENNESSDHVTDNSFNRSSSFLFDSLYDNSILDAMEEAAIDQDNKGDQEAEVKVNSNGSSPELIPERREALPTDDQEAIQWGESSFNLSEWGDSLIIGEQYLEKISKACDGPRLGAEKPSYTDDIVSELNVSQSNEYQQKVERHRLNSSSFHISPGMQDLFDKWSDQFSTVPDATAHSNSLVEPNAVVLEEAVSLTKTKEIEKEGPEVSRSEDFVTVQAKKVTPSDLVPPTQVLEPVTPRVKMTTSAIQSPKNVTKQRSELDPNIKSCTQRYHQPCLRTNSTSVLGAPLADEGLTRLSQCPSLPASNSCSPETFSIIDVASDRRLFETFVNEWKTKERFSLAVACEKTDSTSVQPETVIGGKFKKPTTPMRNKRKDGFLLKGYEDLVVIGISVSWGAKDAYFVSLQQELVDTDISASLAPPPLDDTLTVEERLKQIQSCLQKDSSVTVTYDFIHLYKILLLACELAVRGTFEDPKIACWLLDSSSKERTLHNMVTSFATEDLPMLEGISAGQGVQSLGIYGEASQPGRYRAAIESVLVFRVMTQLNCLLEKDGFLDVFKKVEMPTQYCLALLELNGIGFSIAECEAQKHVMQAKLSALESQAYQLAGHSFSLTSPEDVAEVLFLELKLPPNGDLNGLKNKKTLGYTRRAGARIKLSKQFSTTKDVLEKLKPLHPLPGVILEWRRITNALTKVVFPLQREKKWHSHLKMDRIHPISQSHTATGRVSFTEPNIQNVPKDFEIQMPTLIEESQTSQNGGSKMWCKRTKINRLLAPLLKVSDKSPDKGMQFSVSMRHAFVPFSGGLILAVDYSQLELRILAHLSRDRRLLHVLNSGADVFKSIAAEWKMVDPASVDDNMRQQAKQICYGIIYGMGAKSLGEQMGIEENDAACYIETFKSRYNGIQNFLRETVQKCGKNGYVKTLLGRKRFLPGIKDSNVYIKSHAERQAVNTTVQGSAADIVKLATINIQRRIEEAFPGVPTSHQHPSIRLGGRHRNQFRPLRGGYFILQLHDELIYEVAEEDVIQVAQIVKREMESVVKLYVKLRVKVKVGPSWGNLQDLDI

Target ORF information:

RefSeq Version	XM_009295407.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295407.2

ORF Insert Sequence:

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GATGTTTTAG AGAAGCTCAA GCCATTACAT CCTTTACCAG GTGTTATTCT TGAGTGGAGA 
AGAATCACAA ATGCGTTGAC AAAAGTGGTT TTCCCCCTGC AACGAGAGAA AAAGTGGCAC 
TCACACCTGA AGATGGACAG AATACATCCA ATCTCACAGT CGCACACTGC CACAGGACGT 
GTGAGCTTCA CTGAACCAAA CATTCAAAAT GTGCCAAAAG ATTTTGAAAT TCAGATGCCA 
ACACTTATAG AAGAAAGTCA AACGTCACAG AATGGTGGAA GCAAGATGTG GTGTAAACGA 
ACAAAGATAA ACCGACTGTT GGCACCACTT TTAAAAGTTT CAGACAAATC ACCAGACAAA 
GGGATGCAAT TCTCTGTCAG CATGAGGCAT GCTTTTGTTC CCTTTTCAGG AGGTCTGATA 
TTAGCTGTTG ACTACTCACA ACTGGAGCTC CGCATCTTAG CTCATCTCTC CCGGGACAGA 
AGACTCCTCC ATGTGCTCAA CAGTGGTGCA GATGTGTTTA AAAGCATAGC AGCTGAGTGG 
AAAATGGTGG ACCCTGCTTC AGTTGATGAT AACATGAGGC AGCAGGCCAA ACAGATTTGT 
TATGGAATAA TCTACGGTAT GGGTGCAAAG TCACTCGGAG AGCAAATGGG CATTGAGGAG 
AATGATGCTG CCTGTTACAT TGAGACATTT AAATCCAGAT ACAATGGCAT ACAGAACTTT 
CTGCGTGAAA CTGTGCAAAA GTGTGGGAAA AACGGTTATG TAAAGACATT ACTCGGCAGG 
AAGCGTTTCT TGCCTGGAAT TAAAGACTCA AATGTGTACA TCAAGTCTCA CGCAGAGAGA 
CAGGCAGTAA ACACAACTGT TCAGGGCTCT GCTGCTGACA TTGTGAAATT AGCAACCATC 
AACATCCAGC GCAGAATAGA AGAAGCGTTT CCTGGAGTGC CCACATCACA CCAACACCCT 
TCAATCAGAT TGGGTGGTCG ACACCGAAAT CAGTTTAGAC CCCTTCGAGG AGGCTACTTT 
ATACTTCAAC TGCATGATGA ACTCATCTAC GAGGTCGCAG AGGAAGATGT CATTCAGGTT 
GCACAAATTG TGAAGCGAGA AATGGAGTCT GTTGTTAAAT TATACGTGAA GCTCAGAGTA 
AAAGTTAAAG TAGGGCCAAG CTGGGGTAAC CTTCAAGACC TGGACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_017352975.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208464.1 Danio rerio polymerase (DNA directed), theta (polq), transcript variant X2, mRNA.

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CloneID	ODa56928
Clone ID Related Accession (Same CDS sequence)	XM_017352975.1
Accession Version	XM_017352975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase theta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GTGTTATTCT TGAGTGGAGA AGAATCACAA ATGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208464.1
CDS	326..6904
Translation	MMTATTRKIYLGQHRIVKTRGLSLADEKPGQRQLSNLCQEDVSENRSIIAPKQIQSKQWMPGDSSLAMDEEILQAIDELGPEVMKFKKIEKIQQTEPAPLKKNCDFPKNAEIPKEQNAENISPSSPSEHKRTRTQAKKEGDWKDLAQKLLFSETKKIEVGGRTHDLKQNRDYSSPQKILNSMKKTSKVAKSKQKLNFREVTSPNDSKDYILFSPTHMAAAKERSVLQQQKRSLKNQSVSVLTPPPGLDLTVLGDATLSDAAQSIHMGRPADQLDKLLLSSWGLPKPVLEKYQSLGVHRMFEWQAECLTLGKVLEGQNLVYSAPTSAGKTLVSELLILKRVLETRQKAMFILPFVSVAREKMFYLQNVFQEAGIRVEGYMGSTSAAGGFSALDVAVCTIEKANGLVNRLIEEDKLDLLGTVVVDELHMVGDSGRGYLLELLLTKIRYIAQKTATRNSGQSTSTGVQIIGMSATLPNLDLLARWLSAELYSTNYRPVPLMEWVKIGTNIYDGSLNLVRQFTPALPIKGDDDHIVSLCFETVQDGHSALLFCPSKNWCEKLADSIGREFYNLHHKEMQSGSGGQSISLNQEGLLDVLAQLKRTPAGLDHVLQRTVPWGVAFHHAGLTFDERDILEGAFRQGYIRVLAATSTLSSGVNLPARRVIIRTPVFNGHLLDILTYKQMVGRAGRKGVDTIGESVLVCKEAERAKGMSLIQGSLKPISSCLVKREGEGVTTSMLRAILEIIVGGVASSPQDVRMYAACTLLAASIAAEEVHQDGSESEARNKGAIEACIEWLMDNEFIHIQKEGDVERYCPTHLGSATLSSSLSPPEALGIFADLQRAMKGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSAMKRVAEMVGIQEGFLARSVGGKLIAKTEKQRRQMAIHKRFFTTLVLLDLVSEEPLGAVAKKYGCSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLELLLSQFQSRLSFGVQRELCDLVRISLLTAQRARTLYSSGFVTVAELARADVTEVEKALRKAIPFKSSRQAVDESEVEAQERKSMRCIWVSGKKALTEREAAQQIVAEAQLLLQKDLALLGVEWNPACLPAKTQPDNSSNTEPRSMSGGTEHEGGQDLMLNVTEEKLLLENNSNNNSHPPLRPLESTTNLEKTMDVDSGVSSAECQPDKKCELQSSVLHKVLKSINAKDKGCDNNQTVESSSESLQRRNSCLNTCISGNDQHCVSPVSKRRRMDAAGDEEGPTVTGVKMRIANGLATDRKEMPKPEELCRIDQVKMKLNKDTGLKKDSRSCTPLPNEEVTNVCVVSSGKGKKRTSKMKHLNINADINHETQTEKEEMVAEIYGKFSNRDQNEGDLHVNTRVKNDLVCNSEKLSSPDLYTNGLEEFGDSFQLDTQTEKMLHGHDFSHRNVELNVVRENKQDNQVDSTSEKVVKQSSFLEKENRVSKEQNKGPFESLSLHDNGWPTEAKPKYNISLTDSQLENILNYSNQVPEEEQCANKPAEQRENNESSDHVTDNSFNRSSSFLFDSLYDNSILDAMEEAAIDQDNKGDQEAEVKVNSNGSSPELIPERREALPTDDQEAIQWGESSFNLSEWGDSLIIGEQYLEKISKACDGPRLGAEKPSYTDDIVSELNVSQSNEYQQKVERHRLNSSSFHISPGMQDLFDKWSDQFSTVPDATAHSNSLVEPNAVVLEEAVSLTKTKEIEKEGPEVSRSEDFVTVQAKKVTPSDLVPPTQVLEPVTPRVKMTTSAIQSPKNVTKQRSELDPNIKSCTQRYHQPCLRTNSTSVLGAPLADEGLTRLSQCPSLPASNSCSPETFSIIDVASDRRLFETFVNEWKTKERFSLAVACEKTDSTSVQPETVIGGKFKKPTTPMRNKRKDGFLLKGYEDLVVIGISVSWGAKDAYFVSLQQELVDTDISASLAPPPLDDTLTVEERLKQIQSCLQKDSSVTVTYDFIHLYKILLLACELAVRGTFEDPKIACWLLDSSSKERTLHNMVTSFATEDLPMLEGISAGQGVQSLGIYGEASQPGRYRAAIESVLVFRVMTQLNCLLEKDGFLDVFKKVEMPTQYCLALLELNGIGFSIAECEAQKHVMQAKLSALESQAYQLAGHSFSLTSPEDVAEVLFLELKLPPNGDLNGLKNKKTLGYTRRAGARIKLSKQFSTTKVLFLSGEESQMR

Target ORF information:

RefSeq Version	XM_017352975.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA directed), theta (polq), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352975.1

ORF Insert Sequence:

ATGATGACTG CAACGACAAG AAAGATTTAT TTGGGACAAC ATCGTATTGT GAAGACAAGA 
GGCTTAAGTC TTGCAGATGA GAAGCCCGGA CAAAGACAAT TAAGTAACCT GTGTCAGGAA 
GATGTATCAG AGAACAGAAG CATTATTGCA CCAAAACAAA TCCAGAGTAA GCAGTGGATG 
CCAGGAGACT CCAGTCTGGC TATGGATGAG GAAATCTTAC AAGCCATTGA TGAACTTGGT 
CCAGAAGTCA TGAAGTTCAA GAAGATTGAA AAGATTCAAC AAACCGAACC AGCACCATTA 
AAGAAAAACT GTGACTTTCC AAAAAATGCT GAAATTCCAA AAGAGCAAAA CGCAGAAAAT 
ATCAGCCCTT CAAGTCCATC AGAGCACAAA AGAACCAGAA CTCAAGCCAA AAAAGAGGGT 
GATTGGAAGG ATTTGGCCCA AAAACTTTTG TTCAGCGAAA CCAAAAAGAT TGAAGTGGGA 
GGACGGACCC ATGATTTGAA ACAGAACAGA GATTATTCTT CCCCTCAAAA GATCCTGAAC 
TCCATGAAAA AAACATCTAA AGTAGCAAAG AGCAAGCAGA AGTTAAATTT CAGAGAAGTC 
ACCAGCCCTA ATGACTCCAA GGATTACATT CTGTTCAGTC CAACACATAT GGCTGCTGCT 
AAAGAGCGGT CAGTGTTGCA GCAGCAGAAG AGATCTCTAA AAAACCAATC AGTGTCTGTG 
TTGACCCCTC CGCCTGGATT GGATCTTACC GTCCTCGGTG ATGCTACACT ATCAGATGCA 
GCACAAAGCA TCCACATGGG ACGTCCTGCT GATCAGTTAG ACAAGCTTTT GCTCTCCAGC 
TGGGGTCTTC CTAAGCCTGT TCTGGAGAAG TACCAGAGTC TCGGTGTTCA CAGGATGTTT 
GAGTGGCAGG CTGAGTGTCT GACTCTGGGC AAAGTGCTGG AGGGCCAGAA CCTGGTATAT 
TCTGCTCCAA CCAGTGCAGG AAAAACATTG GTTTCTGAGT TGCTGATCTT GAAGAGAGTG 
CTGGAAACAA GACAGAAAGC CATGTTCATT CTTCCGTTCG TGTCTGTGGC GAGAGAGAAG 
ATGTTTTATC TACAGAATGT ATTTCAGGAG GCAGGGATCA GGGTGGAGGG ATATATGGGG 
AGCACTTCAG CAGCTGGTGG GTTTTCAGCA CTAGATGTCG CTGTTTGCAC AATAGAGAAA 
GCTAACGGAC TCGTCAACAG ACTCATTGAA GAAGATAAAC TGGATTTACT TGGAACTGTT 
GTAGTTGATG AGCTGCACAT GGTTGGGGAC TCTGGTCGTG GATATCTGTT AGAGCTTTTG 
CTTACTAAAA TACGATACAT TGCCCAAAAA ACTGCAACAC GAAACTCTGG TCAGAGTACC 
TCTACTGGTG TGCAGATTAT TGGTATGAGT GCCACGCTGC CAAACTTGGA TCTTCTTGCC 
CGCTGGTTGA GTGCTGAGCT GTATAGTACG AACTATCGCC CAGTGCCACT GATGGAGTGG 
GTCAAGATTG GCACAAATAT TTATGACGGA TCTCTGAATT TGGTCAGACA GTTCACTCCA 
GCTCTTCCCA TTAAGGGAGA CGATGATCAC ATTGTCAGCC TCTGCTTTGA GACCGTACAG 
GACGGACACT CTGCACTGCT GTTTTGCCCT TCGAAGAACT GGTGTGAGAA ACTAGCAGAT 
AGCATTGGAA GAGAGTTTTA CAATCTTCAT CACAAAGAAA TGCAGTCAGG CTCTGGAGGG 
CAGAGTATAT CTCTTAATCA GGAAGGTCTC CTAGATGTAT TAGCTCAGCT GAAACGTACA 
CCCGCAGGTT TGGACCATGT GCTTCAGAGG ACTGTTCCTT GGGGTGTTGC CTTCCATCAT 
GCAGGTTTGA CGTTCGATGA GCGGGATATT CTTGAAGGTG CTTTCCGTCA AGGCTACATT 
AGGGTGTTAG CTGCCACCTC CACCTTGTCT TCTGGTGTAA ATCTGCCCGC TCGACGTGTG 
ATTATCAGAA CTCCTGTTTT TAATGGTCAT TTGTTGGACA TCCTGACCTA TAAGCAGATG 
GTGGGACGGG CAGGCCGTAA AGGCGTGGAC ACAATAGGTG AGAGTGTGTT GGTCTGTAAA 
GAGGCTGAAC GTGCGAAGGG GATGAGCCTC ATCCAGGGAT CGCTTAAGCC TATCAGCAGC 
TGCCTGGTGA AGCGGGAAGG AGAGGGCGTT ACTACCAGCA TGCTAAGGGC CATCCTGGAG 
ATCATTGTTG GAGGAGTTGC CAGCTCACCA CAGGATGTGA GAATGTATGC AGCTTGCACT 
CTGCTGGCGG CCAGTATAGC AGCTGAAGAA GTTCATCAGG ATGGAAGCGA GTCAGAAGCT 
CGAAATAAAG GTGCTATTGA AGCCTGCATC GAGTGGCTGA TGGACAATGA GTTCATCCAT 
ATACAGAAAG AAGGAGATGT GGAGAGGTAC TGTCCTACAC ATTTGGGTTC TGCGACGTTG 
TCTTCCTCTT TATCACCTCC GGAAGCATTG GGGATTTTTG CAGATCTTCA GAGAGCAATG 
AAAGGATTTG TGCTGGAAAA TGATCTCCAT ATCCTTTACC AGATAACCCC TGTGTATGTG 
GACTGGACGA CAATAGACTG GTATCAGTTT TTCTGTCTTT GGGAGCAACT ACCATCGGCA 
ATGAAGCGGG TGGCTGAGAT GGTCGGCATA CAGGAAGGTT TCCTGGCTCG TTCTGTTGGT 
GGCAAATTAA TTGCAAAAAC CGAGAAGCAA CGTAGGCAGA TGGCCATTCA CAAACGCTTT 
TTCACAACGT TAGTTCTTCT TGACTTGGTA AGCGAAGAGC CTCTTGGAGC TGTTGCAAAG 
AAGTATGGCT GTAGCCGAGG ACAGCTTCAG TCACTTCAGC AGTCTGCTTC CACTTATGCA 
GGTATGGTTA CGGTTTTCTG TAATCGGCTT GGATGGCACA ACCTAGAGCT GCTTCTCTCC 
CAGTTCCAGA GTCGTCTCAG TTTTGGAGTC CAGAGGGAGT TGTGTGATCT AGTTCGAATC 
TCTTTACTAA CTGCCCAGAG AGCACGAACA CTTTACAGCT CTGGGTTTGT CACGGTAGCA 
GAGTTGGCCA GAGCTGATGT TACAGAGGTG GAGAAGGCCT TAAGGAAAGC TATTCCTTTT 
AAAAGCTCCA GACAGGCAGT AGATGAGAGC GAGGTGGAGG CTCAAGAACG CAAGAGTATG 
CGCTGTATCT GGGTCAGTGG GAAAAAGGCA TTGACTGAAA GAGAAGCTGC CCAGCAGATC 
GTGGCTGAAG CCCAGCTGCT CCTCCAGAAA GATCTGGCTC TTCTAGGGGT GGAGTGGAAC 
CCAGCTTGCC TCCCAGCAAA GACTCAGCCA GATAACAGTT CAAACACAGA ACCACGGTCC 
ATGTCAGGAG GCACAGAACA TGAAGGGGGC CAAGACTTAA TGTTAAATGT GACTGAAGAG 
AAGTTACTGC TGGAAAATAA TTCTAACAAT AATTCACACC CCCCTTTGAG GCCATTAGAA 
TCCACTACCA ATTTAGAAAA AACCATGGAC GTTGACTCGG GTGTATCATC TGCTGAATGC 
CAACCTGATA AGAAATGTGA ACTGCAATCA AGTGTACTTC ATAAAGTTCT CAAATCTATC 
AATGCAAAAG ACAAGGGCTG TGATAATAAC CAGACAGTTG AATCATCATC TGAATCACTT 
CAGAGGCGAA ATTCATGTCT TAATACCTGC ATCTCTGGCA ATGACCAACA CTGTGTTAGT 
CCAGTTTCAA AACGCAGAAG GATGGATGCT GCAGGTGATG AAGAGGGACC CACAGTCACT 
GGAGTGAAGA TGAGAATTGC AAATGGACTT GCAACAGACC GTAAGGAAAT GCCAAAACCT 
GAAGAGCTTT GTAGAATAGA TCAAGTCAAG ATGAAACTGA ACAAAGACAC AGGGTTAAAA 
AAAGACTCTC GCTCATGTAC TCCACTCCCA AATGAGGAAG TCACAAATGT CTGTGTGGTT 
TCCTCTGGTA AGGGGAAAAA GCGCACATCA AAAATGAAAC ATTTAAATAT AAATGCAGAC 
ATTAATCATG AAACCCAAAC TGAAAAAGAA GAAATGGTTG CAGAAATTTA TGGGAAATTT 
AGCAACAGAG ATCAGAACGA AGGGGACTTG CATGTAAACA CCAGAGTAAA GAATGATTTA 
GTATGTAATT CTGAGAAGCT TAGCTCTCCT GACTTGTACA CAAATGGACT AGAAGAGTTT 
GGTGATAGTT TTCAGCTTGA TACACAGACT GAGAAAATGC TTCACGGGCA TGATTTTTCA 
CATAGGAATG TTGAATTAAA CGTTGTCAGA GAAAATAAAC AGGATAACCA AGTTGATTCT 
ACAAGTGAGA AAGTTGTCAA ACAGTCATCT TTCCTGGAAA AGGAGAATCG AGTATCTAAA 
GAGCAAAACA AAGGCCCATT TGAAAGTTTA TCTCTCCATG ATAATGGCTG GCCCACCGAA 
GCCAAACCAA AATACAACAT TTCACTTACT GACAGCCAGT TAGAGAACAT TTTAAATTAC 
AGTAATCAAG TTCCTGAAGA AGAACAATGT GCGAATAAAC CTGCTGAGCA AAGGGAAAAT 
AATGAGTCTT CAGATCATGT TACTGACAAC AGTTTCAATC GGAGCAGCAG CTTTCTGTTT 
GACAGTCTCT ATGACAACTC CATCTTGGAT GCCATGGAGG AGGCAGCAAT AGATCAAGAC 
AACAAGGGAG ATCAGGAGGC TGAAGTGAAA GTTAATTCAA ATGGCTCTTC TCCAGAACTC 
ATTCCAGAGA GGAGAGAGGC TTTGCCAACA GATGACCAGG AAGCAATCCA ATGGGGGGAA 
TCATCTTTCA ACCTTTCAGA ATGGGGAGAT TCACTAATCA TTGGTGAGCA GTACCTTGAA 
AAAATAAGCA AGGCTTGTGA TGGCCCAAGA TTGGGTGCAG AGAAGCCAAG TTATACAGAT 
GACATAGTGT CTGAACTGAA TGTGTCACAA AGCAATGAAT ATCAGCAAAA AGTTGAAAGG 
CATCGATTAA ATAGCTCATC GTTTCATATC AGTCCAGGAA TGCAGGACTT GTTTGATAAA 
TGGTCTGATC AGTTTTCAAC TGTACCTGAT GCAACAGCTC ACTCAAATTC ATTAGTGGAA 
CCAAATGCTG TTGTCCTTGA AGAAGCTGTT TCCCTAACAA AAACCAAAGA GATTGAGAAG 
GAAGGTCCAG AAGTTTCAAG ATCTGAAGAC TTTGTAACAG TCCAGGCTAA GAAAGTGACT 
CCTAGTGACC TTGTTCCTCC CACACAAGTT TTAGAACCAG TCACACCACG AGTCAAAATG 
ACCACTTCTG CTATACAATC ACCTAAAAAT GTTACAAAGC AAAGGTCTGA ACTAGATCCA 
AATATCAAAT CATGTACACA GAGATATCAT CAACCTTGTC TGAGGACAAA CTCAACATCA 
GTGTTAGGCG CACCTCTGGC TGACGAGGGC TTAACGAGAC TTTCTCAGTG TCCTTCACTT 
CCTGCTTCTA ACTCCTGCAG TCCAGAAACG TTTTCCATAA TTGATGTGGC AAGCGACAGA 
AGGCTTTTTG AAACATTTGT AAACGAATGG AAAACAAAAG AGAGATTTTC TCTTGCTGTT 
GCCTGTGAGA AGACAGACAG CACCTCAGTG CAACCTGAGA CTGTCATAGG TGGAAAATTC 
AAAAAACCTA CTACCCCAAT GAGGAACAAA AGAAAAGATG GCTTCCTTCT GAAGGGATAT 
GAGGATCTTG TGGTTATTGG CATATCTGTA AGCTGGGGAG CAAAAGATGC TTATTTTGTA 
TCTCTACAAC AGGAGCTTGT AGACACAGAT ATAAGTGCCA GTTTAGCGCC ACCGCCTCTT 
GATGATACGC TTACGGTTGA AGAGCGGCTG AAGCAGATTC AGTCCTGCTT GCAAAAAGAC 
TCAAGTGTCA CAGTCACCTA TGACTTCATT CATCTTTACA AAATACTTCT GCTGGCATGT 
GAACTTGCCG TGCGTGGCAC ATTTGAAGAC CCAAAGATTG CATGTTGGCT TTTGGATTCA 
AGCTCCAAAG AGCGTACACT CCACAACATG GTGACCAGTT TCGCAACTGA GGACCTCCCA 
ATGTTGGAGG GAATTAGTGC GGGGCAGGGT GTGCAGAGTT TAGGAATCTA TGGAGAAGCC 
AGTCAACCTG GACGCTACAG AGCTGCTATT GAGTCTGTTC TGGTCTTCAG GGTCATGACC 
CAACTCAACT GTCTACTAGA AAAGGATGGT TTCTTAGATG TATTCAAGAA AGTAGAGATG 
CCAACTCAGT ACTGCCTGGC ATTGTTAGAG CTGAATGGAA TTGGCTTTAG CATTGCCGAA 
TGTGAGGCTC AAAAACATGT CATGCAAGCC AAACTAAGTG CTCTTGAATC CCAAGCTTAT 
CAGCTAGCAG GACACAGCTT CTCATTGACC AGTCCAGAGG ATGTAGCAGA GGTGTTGTTT 
CTTGAGTTGA AGCTGCCTCC AAATGGAGAT TTAAATGGTC TGAAGAATAA GAAGACGCTG 
GGCTACACTA GGAGAGCAGG AGCTCGTATA AAACTTTCTA AACAGTTCAG CACAACCAAG 
GTGTTATTCT TGAGTGGAGA AGAATCACAA ATGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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POLQ cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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