POLQ cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLQ
Entrez Gene ID	10721
Full Name	DNA polymerase theta
Synonyms	PRO0327
General protein information	Preferred Names DNA polymerase theta Names DNA polymerase theta polymerase (DNA directed), theta polymerase (DNA) theta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q13.33
Disorder MIM:	604419

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512352.2	XP_011510654.1	DNA polymerase theta isoform X5
XM_011512343.2	XP_011510645.1	DNA polymerase theta isoform X2
XM_017005565.1	XP_016861054.1	DNA polymerase theta isoform X4
NM_199420.3	NP_955452.3	DNA polymerase theta
NM_199420.4	NP_955452.3	DNA polymerase theta
XM_011512348.2	XP_011510650.1	DNA polymerase theta isoform X3
XM_011512354.2	XP_011510656.1	DNA polymerase theta isoform X6
XM_011512347.2	XP_011510649.1	DNA polymerase theta isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POLQ	XP_545125.3
Gallus gallus (chicken)	POLQ	XP_416549.3
Xenopus tropicalis (tropical clawed frog)	polq	NP_001184156.1
Neurospora crassa	NCU07411	XP_958186.2
Bos taurus (cattle)	POLQ	XP_002684835.1
Rattus norvegicus (Norway rat)	Polq	NP_001099348.1
Danio rerio (zebrafish)	si:rp71-1p14.5	XP_694437.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007533	XP_564726.3
Magnaporthe oryzae (rice blast fungus)	MGG_15295	XP_003711003.1
Homo sapiens (human)	POLQ	NP_955452.3
Macaca mulatta (Rhesus monkey)	POLQ	XP_001111254.2
Drosophila melanogaster (fruit fly)	mus308	NP_524333.1
Pan troglodytes (chimpanzee)	POLQ	XP_001165150.2
Mus musculus (house mouse)	Polq	NP_084253.1

Gene Ontology
Bibliography

Related articles in PubMed

One-step enzymatic modification of RNA 3' termini using polymerase θ.
Thomas C, Rusanov T, Hoang T, Augustin T, Kent T, Gaspar I, Pomerantz RT
Nucleic acids research47(7)3272-3283(2019 04)

DNA polymerase θ accomplishes translesion synthesis opposite 1,N
Yoon JH, Johnson RE, Prakash L, Prakash S
Genes & development33(5-6)282-287(2019 03)

A case of interdigitating dendritic cell sarcoma studied by whole-exome sequencing.
Hong KH, Song S, Shin W, Kang K, Cho CS, Hong YT, Han K, Moon JH
Genes & genomics40(12)1279-1285(2018 12)

Expanded Substrate Scope of DNA Polymerase θ and DNA Polymerase β: Lyase Activity on 5'-Overhangs and Clustered Lesions.
Laverty DJ, Greenberg MM
Biochemistry57(42)6119-6127(2018 10)

DNA Polymerase θ Increases Mutational Rates in Mitochondrial DNA.
Wisnovsky S, Sack T, Pagliarini DJ, Laposa RR, Kelley SO
ACS chemical biology13(4)900-908(2018 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02809	NM_199420.4 Latest version!	Homo sapiens DNA polymerase theta (POLQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu64323	XM_011512354.2 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu64313	XM_011512343.2 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu64317	XM_011512347.2 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu64318	XM_011512348.2 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu64321	XM_011512352.2 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu93745	XM_017005565.1 Latest version!	Homo sapiens DNA polymerase theta (POLQ), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OHu02809	NM_199420.3	Homo sapiens DNA polymerase theta (POLQ), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLQ ( NM_199420.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_955452.3 Homo sapiens DNA polymerase theta (POLQ), mRNA.

Price & Availability↑

CloneID	OHu02809
Clone ID Related Accession (Same CDS sequence)	NM_199420.3 , NM_199420.4
Accession Version	NM_199420.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Homo sapiens(human)
Product	DNA polymerase theta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY338826.2, AC069239.16, AB209867.1, AC079841.10 and AA767021.1. On Apr 3, 2007 this sequence version replaced NM_199420.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY338826.2, AB209867.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGGATTGTC ACGAAAGAGA 
AAACTGCAAG GGATGTTCTG CCCAATCAGA GGAGGCTTCT TCATCCTTCA ACTCCATGAT 
GAACTCCTAT ATGAAGTGGC AGAAGAAGAT GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT 
GAAATGGAAA GTGCTGTAAA ACTGTCTGTG AAATTGAAAG TGAAAGTGAA AATAGGCGCC 
AGCTGGGGAG AGCTAAAGGA CTTTGATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955452.3
CDS	130..7902
Translation	MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	NM_199420.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199420.3

ORF Insert Sequence:

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGGATTGTC ACGAAAGAGA 
AAACTGCAAG GGATGTTCTG CCCAATCAGA GGAGGCTTCT TCATCCTTCA ACTCCATGAT 
GAACTCCTAT ATGAAGTGGC AGAAGAAGAT GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT 
GAAATGGAAA GTGCTGTAAA ACTGTCTGTG AAATTGAAAG TGAAAGTGAA AATAGGCGCC 
AGCTGGGGAG AGCTAAAGGA CTTTGATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( NM_199420.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_955452.3 Homo sapiens DNA polymerase theta (POLQ), mRNA.

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CloneID	OHu02809
Clone ID Related Accession (Same CDS sequence)	NM_199420.3 , NM_199420.4
Accession Version	NM_199420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Homo sapiens(human)
Product	DNA polymerase theta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY338826.2, AC069239.16, AB209867.1 and AC079841.10. On Nov 22, 2018 this sequence version replaced NM_199420.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY338826.2, AB209867.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264233.6/ ENSP00000264233.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGGATTGTC ACGAAAGAGA 
AAACTGCAAG GGATGTTCTG CCCAATCAGA GGAGGCTTCT TCATCCTTCA ACTCCATGAT 
GAACTCCTAT ATGAAGTGGC AGAAGAAGAT GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT 
GAAATGGAAA GTGCTGTAAA ACTGTCTGTG AAATTGAAAG TGAAAGTGAA AATAGGCGCC 
AGCTGGGGAG AGCTAAAGGA CTTTGATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955452.3
CDS	112..7884
Translation	MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	NM_199420.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199420.4

ORF Insert Sequence:

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGGATTGTC ACGAAAGAGA 
AAACTGCAAG GGATGTTCTG CCCAATCAGA GGAGGCTTCT TCATCCTTCA ACTCCATGAT 
GAACTCCTAT ATGAAGTGGC AGAAGAAGAT GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT 
GAAATGGAAA GTGCTGTAAA ACTGTCTGTG AAATTGAAAG TGAAAGTGAA AATAGGCGCC 
AGCTGGGGAG AGCTAAAGGA CTTTGATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_011512354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510656.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X8, mRNA.

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CloneID	OHu64323
Clone ID Related Accession (Same CDS sequence)	XM_011512354.2
Accession Version	XM_011512354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA TCGTGGGCAG ATTCAATCTT TGCAACAGTC AGCTGCTGTT TATGCAGAAG 
CGTCTTACGT TTGGCATCCA GAGGGAGCTG TGTGACCTGG TTCGGGTATC CTTACTAAAT 
GCTCAGAGAG CCAGGGTTCT CTATGCTTCT GGCTTTCATA CTGTGGCAGA CCTTGCTAGA 
GCAAATATTG TGGAGGTGGA GGTGATTCTG AAAAATGCTG TGCCTTTCAA AAGTGCCCGG 
AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAACGTC GCAATATGCG AACTATCTGG 
GTGACTGGCA GAAAAGGTTT AACTGAAAGG GAAGCAGCAG CCCTTATAGT GGAAGAAGCC 
AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCC ATGTGCCCTG 
TTACATTCTA GTACATGCTC ATTGACTCAT AGTGAGTCCG AAGTAAAGGA ACACACATTT 
ATATCCCAAA CTAAGAGTTC TTATAAAAAA TTAACATCAA AGAACAAAAG TAACACAATA 
TTTAGTGATT CTTATATTAA GCATTCACCA AATATAGTGC AAGACTTAAA TAAAAGTAGA 
GAGCATACAA GTTCCTTTAA TTGTAATTTC CAGAATGGGA ATCAAGAACA TCAGACATGT 
TCCATTTTCA GAGCAAGAAA ACGGGCCTCT TTAGATATAA ATAAAGAGAA GCCAGGAGCC 
TCTCAGAATG AGGGGAAAAC AAGTGATAAG AAAGTTGTTC AGACTTTTTC ACAGAAAACA 
AAAAAGGCAC CTTTGAATTT CAATTCAGAA AAGATGAGCA GAAGTTTTCG ATCTTGGAAA 
CGTAGAAAGC ATCTAAAGCG ATCTAGGGAC AGCAGCCCCC TGAAAGACTC TGGAGCGTGT 
AGAATCCATT TACAAGGACA GACTCTGTCT AATCCTAGTC TTTGTGAAGA CCCGTTTACC 
TTAGATGAGA AGAAAACGGA ATTTAGAAAT TCAGGGCCAT TTGCTAAAAA TGTATCTTTG 
AGTGGTAAGG AAAAAGATAA TAAAACATCA TTCCCATTAC AAATAAAGCA AAATTGTTCA 
TGGAACATAA CACTAACTAA TGATAATTTT GTGGAGCATA TTGTCACAGG ATCTCAGAGT 
AAAAATGTGA CTTGTCAGGC CACTAGTGTG GTTAGTGAAA AGGGCAGAGG AGTAGCTGTT 
GAGGCAGAAA AAATAAATGA AGTGCTGATA CAAAATGGTT CAAAAAACCA GAATGTTTAT 
ATGAAACACC ATGACATCCA TCCAATTAAC CAGTACCTGC GAAAGCAATC TCATGAACAG 
ACAAGCACTA TTACCAAACA GAAAAATATA ATAGAGAGAC AAATGCCCTG TGAAGCAGTC 
AGTAGTTACA TAAATAGAGA CTCAAATGTT ACTATCAATT GTGAAAGGAT AAAGCTTAAT 
ACAGAGGAAA ATAAACCAAG TCATTTTCAG GCATTAGGAG ATGATATAAG CAGAACTGTG 
ATACCCAGTG AAGTACTTCC ATCAGCTGGA GCATTTAGCA AATCAGAAGG CCAGCATGAG 
AATTTTCTAA ATATTTCTAG ACTACAAGAA AAAACAGGTA CTTATACAAC AAACAAAACT 
AAAAATAATC ATGTTTCTGA CTTAGGTTTA GTCCTCTGTG ATTTTGAAGA TAGTTTCTAT 
CTGGATACTC AGTCAGAGAA AATAATACAA CAGATGGCAA CTGAAAATGC CAAACTAGGA 
GCAAAGGACA CCAACCTGGC AGCAGGGATA ATGCAGAAGA GCTTAGTCCA ACAGAACTCA 
ATGAACTCTT TTCAGAAGGA GTGTCACATT CCTTTTCCTG CTGAACAGCA CCCTCTAGGA 
GCGACTAAGA TAGATCATTT GGACCTTAAG ACTGTAGGTA CTATGAAACA AAGCAGTGAT 
TCACATGGGG TTGATATCCT GACTCCAGAA AGCCCGATTT TCCATTCTCC AATACTATTG 
GAGGAAAATG GTCTTTTTTT AAAAAAGAAT GAAGTTTCTG TTACTGATTC ACAATTAAAT 
AGTTTTCTTC AAGGTTATCA AACACAAGAA ACTGTGAAAC CAGTTATACT TCTGATTCCT 
CAAAAGAGAA CTCCCACTGG TGTAGAAGGA GAATGTCTTC CAGTTCCTGA AACAAGTTTG 
AATATGAGTG ATAGTTTACT ATTTGATAGC TTCAGTGATG ACTATCTAGT AAAAGAACAA 
TTACCTGATA TGCAAATGAA AGAACCCCTT CCTTCAGAAG TAACATCAAA CCATTTTAGT 
GATTCTCTGT GTCTACAAGA AGACCTAATT AAAAAATCAA ATGTAAATGA GAATCAAGAT 
ACCCACCAGC AGTTGACTTG TTCCAATGAT GAATCTATTA TATTTTCAGA AATGGATTCT 
GTTCAGATGG TTGAAGCTTT GGACAATGTG GATATATTTC CTGTCCAAGA GAAGAATCAT 
ACTGTAGTAT CTCCTAGAGC ATTAGAACTA AGTGATCCAG TACTTGATGA GCACCACCAA 
GGTGATCAAG ATGGAGGAGA TCAAGATGAA AGGGCTGAAA AATCAAAATT AACTGGGACC 
AGGCAAAATC ATTCATTCAT ATGGTCAGGG GCATCATTTG ATCTAAGTCC AGGACTGCAA 
AGGATTTTAG ATAAAGTATC CAGTCCTCTA GAAAATGAAA AGCTAAAATC AATGACTATA 
AACTTTTCCA GTTTGAATAG AAAAAATACA GAGTTAAATG AAGAACAAGA AGTTATTTCA 
AACTTGGAGA CAAAACAAGT GCAGGGAATT TCATTTTCTT CTAATAATGA AGTAAAAAGC 
AAGATTGAGA TGCTAGAAAA CAATGCCAAT CATGATGAAA CCTCATCCCT CTTACCTCGT 
AAAGAAAGTA ATATAGTTGA TGATAATGGT CTCATTCCTC CTACACCCAT TCCAACATCT 
GCTTCTAAGC TGACATTTCC AGGGATTCTT GAAACACCTG TAAACCCTTG GAAAACTAAT 
AATGTTTTAC AACCTGGTGA AAGTTATTTA TTTGGCTCAC CTTCAGATAT TAAAAACCAC 
GATTTAAGTC CAGGGAGTAG AAATGGGTTC AAAGACAACA GCCCTATTAG TGACACAAGC 
TTTTCACTTC AGTTATCACA GGATGGATTA CAGTTAACTC CAGCCTCAAG CAGTTCAGAA 
AGTTTGTCCA TAATTGATGT AGCAAGTGAC CAAAATCTTT TCCAAACATT CATTAAGGAG 
TGGCGGTGCA AAAAGCGATT TTCCATCTCA CTGGCTTGTG AAAAGATTAG AAGTTTGACA 
TCTTCTAAAA CTGCTACTAT TGGCAGTAGG TTTAAGCAAG CTAGCTCACC TCAGGAAATT 
CCTATTAGAG ATGATGGATT TCCCATTAAA GGTTGTGATG ACACCTTGGT GGTTGGACTG 
GCAGTATGCT GGGGTGGAAG GGATGCCTAT TATTTTTCAC TGCAGAAGGA ACAAAAGCAT 
TCTGAAATTA GTGCCAGTTT GGTTCCACCT TCTTTAGATC CAAGCCTGAC TTTGAAAGAC 
AGGATGTGGT ACCTTCAATC TTGCTTGCGA AAGGAATCTG ATAAAGAATG TTCTGTTGTC 
ATCTATGACT TCATCCAGAG CTATAAAATT CTTCTTCTTT CTTGTGGCAT CTCCTTGGAG 
CAAAGTTATG AAGATCCTAA GGTGGCATGC TGGTTACTAG ATCCAGATTC TCAGGAGCCG 
ACTCTTCATA GCATAGTTAC CAGTTTTCTT CCTCATGAGC TTCCACTCCT AGAAGGGATG 
GAGACCAGCC AAGGGATTCA AAGCCTGGGG CTAAATGCTG GCAGTGAGCA TTCTGGGCGA 
TACAGAGCAT CTGTGGAGTC CATTCTCATC TTCAACTCTA TGAATCAGCT CAACTCTTTG 
TTGCAGAAGG AAAACCTTCA AGATGTTTTC CGTAAGGTGG AAATGCCCTC TCAGTACTGC 
TTGGCCTTGC TAGAACTAAA TGGAATTGGC TTTAGTACTG CAGAATGTGA AAGTCAGAAA 
CATATAATGC AAGCCAAGCT GGATGCAATT GAGACCCAGG CCTATCAACT AGCTGGCCAC 
AGTTTTTCTT TCACCAGTTC AGATGACATC GCTGAGGTTT TATTTTTGGA ATTGAAGTTG 
CCCCCAAATA GAGAGATGAA AAACCAAGGC AGCAAGAAAA CTCTGGGTTC TACCAGAAGA 
GGGATTGACA ATGGACGCAA GCTAAGGCTG GGAAGACAGT TCAGCACTAG TAAGGACGTT 
TTAAATAAAT TAAAGGCATT ACATCCTTTA CCAGGCTTGA TATTAGAATG GAGAAGAATC 
ACTAATGCTA TTACCAAAGT GGTCTTTCCC CTTCAGCGGG AAAAGTGTCT TAATCCTTTT 
CTTGGAATGG AAAGAATCTA TCCTGTATCA CAGTCGCACA CTGCTACAGG ACGAATAACC 
TTTACAGAAC CAAATATTCA GAATGTGCCA AGAGATTTTG AAATCAAAAT GCCAACACTA 
GTAGGAGAAA GCCCACCTTC TCAAGCTGTA GGCAAAGGCC TACTTCCCAT GGGCAGAGGA 
AAATATAAGA AGGGTTTCAG CGTGAATCCT AGATGCCAGG CACAGATGGA GGAGAGAGCT 
GCAGACAGAG GAATGCCATT TTCAATTAGC ATGCGACATG CCTTTGTGCC TTTCCCAGGT 
GGTTCAATAC TGGCTGCTGA CTACTCTCAG CTTGAACTGA GGATCTTGGC TCATTTATCC 
CATGATCGTC GTCTCATTCA AGTGTTAAAC ACTGGAGCTG ATGTTTTCAG GAGCATTGCA 
GCAGAGTGGA AGATGATTGA GCCAGAGTCT GTTGGGGATG ATCTGAGGCA GCAGGCAAAA 
CAGATTTGCT ATGGGATCAT TTATGGAATG GGAGCTAAAT CTTTGGGAGA GCAGATGGGC 
ATTAAAGAAA ATGATGCTGC ATGCTATATT GACTCCTTCA AATCCAGATA CACAGGGATT 
AATCAATTCA TGACAGAGAC AGTGAAGAAT TGTAAAAGAG ACGGATTTGT TCAGACCATT 
TTGGGAAGGC GTAGATATTT GCCAGGAATC AAAGACAACA ACCCTTATCG TAAAGCTCAC 
GCTGAGCGTC AAGCTATCAA CACAATAGTC CAAGGATCAG CAGCTGATAT TGTCAAAATA 
GCCACAGTTA ACATTCAGAA GCAATTAGAG ACCTTCCACT CAACCTTCAA ATCCCATGGT 
CATCGAGAGG GTATGCTCCA AAGTGACCAA ACAGTAGGAT TGTCACGAAA GAGAAAACTG 
CAAGGGATGT TCTGCCCAAT CAGAGGAGGC TTCTTCATCC TTCAACTCCA TGATGAACTC 
CTATATGAAG TGGCAGAAGA AGATGTTGTT CAGGTAGCTC AGATTGTCAA GAATGAAATG 
GAAAGTGCTG TAAAACTGTC TGTGAAATTG AAAGTGAAAG TGAAAATAGG CGCCAGCTGG 
GGAGAGCTAA AGGACTTTGA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510656.1
CDS	165..5651
Translation	MDAIVGRFNLCNSQLLFMQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTVGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	XM_011512354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512354.2

ORF Insert Sequence:

ATGGATGCAA TCGTGGGCAG ATTCAATCTT TGCAACAGTC AGCTGCTGTT TATGCAGAAG 
CGTCTTACGT TTGGCATCCA GAGGGAGCTG TGTGACCTGG TTCGGGTATC CTTACTAAAT 
GCTCAGAGAG CCAGGGTTCT CTATGCTTCT GGCTTTCATA CTGTGGCAGA CCTTGCTAGA 
GCAAATATTG TGGAGGTGGA GGTGATTCTG AAAAATGCTG TGCCTTTCAA AAGTGCCCGG 
AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAACGTC GCAATATGCG AACTATCTGG 
GTGACTGGCA GAAAAGGTTT AACTGAAAGG GAAGCAGCAG CCCTTATAGT GGAAGAAGCC 
AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCC ATGTGCCCTG 
TTACATTCTA GTACATGCTC ATTGACTCAT AGTGAGTCCG AAGTAAAGGA ACACACATTT 
ATATCCCAAA CTAAGAGTTC TTATAAAAAA TTAACATCAA AGAACAAAAG TAACACAATA 
TTTAGTGATT CTTATATTAA GCATTCACCA AATATAGTGC AAGACTTAAA TAAAAGTAGA 
GAGCATACAA GTTCCTTTAA TTGTAATTTC CAGAATGGGA ATCAAGAACA TCAGACATGT 
TCCATTTTCA GAGCAAGAAA ACGGGCCTCT TTAGATATAA ATAAAGAGAA GCCAGGAGCC 
TCTCAGAATG AGGGGAAAAC AAGTGATAAG AAAGTTGTTC AGACTTTTTC ACAGAAAACA 
AAAAAGGCAC CTTTGAATTT CAATTCAGAA AAGATGAGCA GAAGTTTTCG ATCTTGGAAA 
CGTAGAAAGC ATCTAAAGCG ATCTAGGGAC AGCAGCCCCC TGAAAGACTC TGGAGCGTGT 
AGAATCCATT TACAAGGACA GACTCTGTCT AATCCTAGTC TTTGTGAAGA CCCGTTTACC 
TTAGATGAGA AGAAAACGGA ATTTAGAAAT TCAGGGCCAT TTGCTAAAAA TGTATCTTTG 
AGTGGTAAGG AAAAAGATAA TAAAACATCA TTCCCATTAC AAATAAAGCA AAATTGTTCA 
TGGAACATAA CACTAACTAA TGATAATTTT GTGGAGCATA TTGTCACAGG ATCTCAGAGT 
AAAAATGTGA CTTGTCAGGC CACTAGTGTG GTTAGTGAAA AGGGCAGAGG AGTAGCTGTT 
GAGGCAGAAA AAATAAATGA AGTGCTGATA CAAAATGGTT CAAAAAACCA GAATGTTTAT 
ATGAAACACC ATGACATCCA TCCAATTAAC CAGTACCTGC GAAAGCAATC TCATGAACAG 
ACAAGCACTA TTACCAAACA GAAAAATATA ATAGAGAGAC AAATGCCCTG TGAAGCAGTC 
AGTAGTTACA TAAATAGAGA CTCAAATGTT ACTATCAATT GTGAAAGGAT AAAGCTTAAT 
ACAGAGGAAA ATAAACCAAG TCATTTTCAG GCATTAGGAG ATGATATAAG CAGAACTGTG 
ATACCCAGTG AAGTACTTCC ATCAGCTGGA GCATTTAGCA AATCAGAAGG CCAGCATGAG 
AATTTTCTAA ATATTTCTAG ACTACAAGAA AAAACAGGTA CTTATACAAC AAACAAAACT 
AAAAATAATC ATGTTTCTGA CTTAGGTTTA GTCCTCTGTG ATTTTGAAGA TAGTTTCTAT 
CTGGATACTC AGTCAGAGAA AATAATACAA CAGATGGCAA CTGAAAATGC CAAACTAGGA 
GCAAAGGACA CCAACCTGGC AGCAGGGATA ATGCAGAAGA GCTTAGTCCA ACAGAACTCA 
ATGAACTCTT TTCAGAAGGA GTGTCACATT CCTTTTCCTG CTGAACAGCA CCCTCTAGGA 
GCGACTAAGA TAGATCATTT GGACCTTAAG ACTGTAGGTA CTATGAAACA AAGCAGTGAT 
TCACATGGGG TTGATATCCT GACTCCAGAA AGCCCGATTT TCCATTCTCC AATACTATTG 
GAGGAAAATG GTCTTTTTTT AAAAAAGAAT GAAGTTTCTG TTACTGATTC ACAATTAAAT 
AGTTTTCTTC AAGGTTATCA AACACAAGAA ACTGTGAAAC CAGTTATACT TCTGATTCCT 
CAAAAGAGAA CTCCCACTGG TGTAGAAGGA GAATGTCTTC CAGTTCCTGA AACAAGTTTG 
AATATGAGTG ATAGTTTACT ATTTGATAGC TTCAGTGATG ACTATCTAGT AAAAGAACAA 
TTACCTGATA TGCAAATGAA AGAACCCCTT CCTTCAGAAG TAACATCAAA CCATTTTAGT 
GATTCTCTGT GTCTACAAGA AGACCTAATT AAAAAATCAA ATGTAAATGA GAATCAAGAT 
ACCCACCAGC AGTTGACTTG TTCCAATGAT GAATCTATTA TATTTTCAGA AATGGATTCT 
GTTCAGATGG TTGAAGCTTT GGACAATGTG GATATATTTC CTGTCCAAGA GAAGAATCAT 
ACTGTAGTAT CTCCTAGAGC ATTAGAACTA AGTGATCCAG TACTTGATGA GCACCACCAA 
GGTGATCAAG ATGGAGGAGA TCAAGATGAA AGGGCTGAAA AATCAAAATT AACTGGGACC 
AGGCAAAATC ATTCATTCAT ATGGTCAGGG GCATCATTTG ATCTAAGTCC AGGACTGCAA 
AGGATTTTAG ATAAAGTATC CAGTCCTCTA GAAAATGAAA AGCTAAAATC AATGACTATA 
AACTTTTCCA GTTTGAATAG AAAAAATACA GAGTTAAATG AAGAACAAGA AGTTATTTCA 
AACTTGGAGA CAAAACAAGT GCAGGGAATT TCATTTTCTT CTAATAATGA AGTAAAAAGC 
AAGATTGAGA TGCTAGAAAA CAATGCCAAT CATGATGAAA CCTCATCCCT CTTACCTCGT 
AAAGAAAGTA ATATAGTTGA TGATAATGGT CTCATTCCTC CTACACCCAT TCCAACATCT 
GCTTCTAAGC TGACATTTCC AGGGATTCTT GAAACACCTG TAAACCCTTG GAAAACTAAT 
AATGTTTTAC AACCTGGTGA AAGTTATTTA TTTGGCTCAC CTTCAGATAT TAAAAACCAC 
GATTTAAGTC CAGGGAGTAG AAATGGGTTC AAAGACAACA GCCCTATTAG TGACACAAGC 
TTTTCACTTC AGTTATCACA GGATGGATTA CAGTTAACTC CAGCCTCAAG CAGTTCAGAA 
AGTTTGTCCA TAATTGATGT AGCAAGTGAC CAAAATCTTT TCCAAACATT CATTAAGGAG 
TGGCGGTGCA AAAAGCGATT TTCCATCTCA CTGGCTTGTG AAAAGATTAG AAGTTTGACA 
TCTTCTAAAA CTGCTACTAT TGGCAGTAGG TTTAAGCAAG CTAGCTCACC TCAGGAAATT 
CCTATTAGAG ATGATGGATT TCCCATTAAA GGTTGTGATG ACACCTTGGT GGTTGGACTG 
GCAGTATGCT GGGGTGGAAG GGATGCCTAT TATTTTTCAC TGCAGAAGGA ACAAAAGCAT 
TCTGAAATTA GTGCCAGTTT GGTTCCACCT TCTTTAGATC CAAGCCTGAC TTTGAAAGAC 
AGGATGTGGT ACCTTCAATC TTGCTTGCGA AAGGAATCTG ATAAAGAATG TTCTGTTGTC 
ATCTATGACT TCATCCAGAG CTATAAAATT CTTCTTCTTT CTTGTGGCAT CTCCTTGGAG 
CAAAGTTATG AAGATCCTAA GGTGGCATGC TGGTTACTAG ATCCAGATTC TCAGGAGCCG 
ACTCTTCATA GCATAGTTAC CAGTTTTCTT CCTCATGAGC TTCCACTCCT AGAAGGGATG 
GAGACCAGCC AAGGGATTCA AAGCCTGGGG CTAAATGCTG GCAGTGAGCA TTCTGGGCGA 
TACAGAGCAT CTGTGGAGTC CATTCTCATC TTCAACTCTA TGAATCAGCT CAACTCTTTG 
TTGCAGAAGG AAAACCTTCA AGATGTTTTC CGTAAGGTGG AAATGCCCTC TCAGTACTGC 
TTGGCCTTGC TAGAACTAAA TGGAATTGGC TTTAGTACTG CAGAATGTGA AAGTCAGAAA 
CATATAATGC AAGCCAAGCT GGATGCAATT GAGACCCAGG CCTATCAACT AGCTGGCCAC 
AGTTTTTCTT TCACCAGTTC AGATGACATC GCTGAGGTTT TATTTTTGGA ATTGAAGTTG 
CCCCCAAATA GAGAGATGAA AAACCAAGGC AGCAAGAAAA CTCTGGGTTC TACCAGAAGA 
GGGATTGACA ATGGACGCAA GCTAAGGCTG GGAAGACAGT TCAGCACTAG TAAGGACGTT 
TTAAATAAAT TAAAGGCATT ACATCCTTTA CCAGGCTTGA TATTAGAATG GAGAAGAATC 
ACTAATGCTA TTACCAAAGT GGTCTTTCCC CTTCAGCGGG AAAAGTGTCT TAATCCTTTT 
CTTGGAATGG AAAGAATCTA TCCTGTATCA CAGTCGCACA CTGCTACAGG ACGAATAACC 
TTTACAGAAC CAAATATTCA GAATGTGCCA AGAGATTTTG AAATCAAAAT GCCAACACTA 
GTAGGAGAAA GCCCACCTTC TCAAGCTGTA GGCAAAGGCC TACTTCCCAT GGGCAGAGGA 
AAATATAAGA AGGGTTTCAG CGTGAATCCT AGATGCCAGG CACAGATGGA GGAGAGAGCT 
GCAGACAGAG GAATGCCATT TTCAATTAGC ATGCGACATG CCTTTGTGCC TTTCCCAGGT 
GGTTCAATAC TGGCTGCTGA CTACTCTCAG CTTGAACTGA GGATCTTGGC TCATTTATCC 
CATGATCGTC GTCTCATTCA AGTGTTAAAC ACTGGAGCTG ATGTTTTCAG GAGCATTGCA 
GCAGAGTGGA AGATGATTGA GCCAGAGTCT GTTGGGGATG ATCTGAGGCA GCAGGCAAAA 
CAGATTTGCT ATGGGATCAT TTATGGAATG GGAGCTAAAT CTTTGGGAGA GCAGATGGGC 
ATTAAAGAAA ATGATGCTGC ATGCTATATT GACTCCTTCA AATCCAGATA CACAGGGATT 
AATCAATTCA TGACAGAGAC AGTGAAGAAT TGTAAAAGAG ACGGATTTGT TCAGACCATT 
TTGGGAAGGC GTAGATATTT GCCAGGAATC AAAGACAACA ACCCTTATCG TAAAGCTCAC 
GCTGAGCGTC AAGCTATCAA CACAATAGTC CAAGGATCAG CAGCTGATAT TGTCAAAATA 
GCCACAGTTA ACATTCAGAA GCAATTAGAG ACCTTCCACT CAACCTTCAA ATCCCATGGT 
CATCGAGAGG GTATGCTCCA AAGTGACCAA ACAGTAGGAT TGTCACGAAA GAGAAAACTG 
CAAGGGATGT TCTGCCCAAT CAGAGGAGGC TTCTTCATCC TTCAACTCCA TGATGAACTC 
CTATATGAAG TGGCAGAAGA AGATGTTGTT CAGGTAGCTC AGATTGTCAA GAATGAAATG 
GAAAGTGCTG TAAAACTGTC TGTGAAATTG AAAGTGAAAG TGAAAATAGG CGCCAGCTGG 
GGAGAGCTAA AGGACTTTGA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_011512343.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510645.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X2, mRNA.

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CloneID	OHu64313
Clone ID Related Accession (Same CDS sequence)	XM_011512343.2
Accession Version	XM_011512343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGACGT TTTAAATAAA 
TTAAAGGCAT TACATCCTTT ACCAGGCTTG ATATTAGAAT GGAGAAGAAT CACTAATGCT 
ATTACCAAAG TGGTCTTTCC CCTTCAGCGG GAAAAGTGTC TTAATCCTTT TCTTGGAATG 
GAAAGAATCT ATCCTGTATC ACAGTCGCAC ACTGCTACAG GACGAATAAC CTTTACAGAA 
CCAAATATTC AGAATGTGCC AAGAGATTTT GAAATCAAAA TGCCAACACT AGTAGGAGAA 
AGCCCACCTT CTCAAGCTGT AGGCAAAGGC CTACTTCCCA TGGGCAGAGG AAAATATAAG 
AAGGGTTTCA GCGTGAATCC TAGATGCCAG GCACAGATGG AGGAGAGAGC TGCAGACAGA 
GGAATGCCAT TTTCAATTAG CATGCGACAT GCCTTTGTGC CTTTCCCAGG TGGTTCAATA 
CTGGCTGCTG ACTACTCTCA GCTTGAACTG AGGATCTTGG CTCATTTATC CCATGATCGT 
CGTCTCATTC AAGTGTTAAA CACTGGAGCT GATGTTTTCA GGAGCATTGC AGCAGAGTGG 
AAGATGATTG AGCCAGAGTC TGTTGGGGAT GATCTGAGGC AGCAGGCAAA ACAGATTTGC 
TATGGGATCA TTTATGGAAT GGGAGCTAAA TCTTTGGGAG AGCAGATGGG CATTAAAGAA 
AATGATGCTG CATGCTATAT TGACTCCTTC AAATCCAGAT ACACAGGGAT TAATCAATTC 
ATGACAGAGA CAGTGAAGAA TTGTAAAAGA GACGGATTTG TTCAGACCAT TTTGGGAAGG 
CGTAGATATT TGCCAGGAAT CAAAGACAAC AACCCTTATC GTAAAGCTCA CGCTGAGCGT 
CAAGCTATCA ACACAATAGT CCAAGGATCA GCAGCTGATA TTGTCAAAAT AGCCACAGTT 
AACATTCAGA AGCAATTAGA GACCTTCCAC TCAACCTTCA AATCCCATGG TCATCGAGAG 
GGTATGCTCC AAAGTGACCA AACAGTAGGA TTGTCACGAA AGAGAAAACT GCAAGGGATG 
TTCTGCCCAA TCAGAGGAGG CTTCTTCATC CTTCAACTCC ATGATGAACT CCTATATGAA 
GTGGCAGAAG AAGATGTTGT TCAGGTAGCT CAGATTGTCA AGAATGAAAT GGAAAGTGCT 
GTAAAACTGT CTGTGAAATT GAAAGTGAAA GTGAAAATAG GCGCCAGCTG GGGAGAGCTA 
AAGGACTTTG ATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510645.1
CDS	118..7755
Translation	MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTVGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	XM_011512343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512343.2

ORF Insert Sequence:

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGACGT TTTAAATAAA 
TTAAAGGCAT TACATCCTTT ACCAGGCTTG ATATTAGAAT GGAGAAGAAT CACTAATGCT 
ATTACCAAAG TGGTCTTTCC CCTTCAGCGG GAAAAGTGTC TTAATCCTTT TCTTGGAATG 
GAAAGAATCT ATCCTGTATC ACAGTCGCAC ACTGCTACAG GACGAATAAC CTTTACAGAA 
CCAAATATTC AGAATGTGCC AAGAGATTTT GAAATCAAAA TGCCAACACT AGTAGGAGAA 
AGCCCACCTT CTCAAGCTGT AGGCAAAGGC CTACTTCCCA TGGGCAGAGG AAAATATAAG 
AAGGGTTTCA GCGTGAATCC TAGATGCCAG GCACAGATGG AGGAGAGAGC TGCAGACAGA 
GGAATGCCAT TTTCAATTAG CATGCGACAT GCCTTTGTGC CTTTCCCAGG TGGTTCAATA 
CTGGCTGCTG ACTACTCTCA GCTTGAACTG AGGATCTTGG CTCATTTATC CCATGATCGT 
CGTCTCATTC AAGTGTTAAA CACTGGAGCT GATGTTTTCA GGAGCATTGC AGCAGAGTGG 
AAGATGATTG AGCCAGAGTC TGTTGGGGAT GATCTGAGGC AGCAGGCAAA ACAGATTTGC 
TATGGGATCA TTTATGGAAT GGGAGCTAAA TCTTTGGGAG AGCAGATGGG CATTAAAGAA 
AATGATGCTG CATGCTATAT TGACTCCTTC AAATCCAGAT ACACAGGGAT TAATCAATTC 
ATGACAGAGA CAGTGAAGAA TTGTAAAAGA GACGGATTTG TTCAGACCAT TTTGGGAAGG 
CGTAGATATT TGCCAGGAAT CAAAGACAAC AACCCTTATC GTAAAGCTCA CGCTGAGCGT 
CAAGCTATCA ACACAATAGT CCAAGGATCA GCAGCTGATA TTGTCAAAAT AGCCACAGTT 
AACATTCAGA AGCAATTAGA GACCTTCCAC TCAACCTTCA AATCCCATGG TCATCGAGAG 
GGTATGCTCC AAAGTGACCA AACAGTAGGA TTGTCACGAA AGAGAAAACT GCAAGGGATG 
TTCTGCCCAA TCAGAGGAGG CTTCTTCATC CTTCAACTCC ATGATGAACT CCTATATGAA 
GTGGCAGAAG AAGATGTTGT TCAGGTAGCT CAGATTGTCA AGAATGAAAT GGAAAGTGCT 
GTAAAACTGT CTGTGAAATT GAAAGTGAAA GTGAAAATAG GCGCCAGCTG GGGAGAGCTA 
AAGGACTTTG ATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_011512347.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510649.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X1, mRNA.

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CloneID	OHu64317
Clone ID Related Accession (Same CDS sequence)	XM_011512347.2
Accession Version	XM_011512347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGTAGGATT GTCACGAAAG 
AGAAAACTGC AAGGGATGTT CTGCCCAATC AGAGGAGGCT TCTTCATCCT TCAACTCCAT 
GATGAACTCC TATATGAAGT GGCAGAAGAA GATGTTGTTC AGGTAGCTCA GATTGTCAAG 
AATGAAATGG AAAGTGCTGT AAAACTGTCT GTGAAATTGA AAGTGAAAGT GAAAATAGGC 
GCCAGCTGGG GAGAGCTAAA GGACTTTGAT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510649.1
CDS	118..7893
Translation	MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTVGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	XM_011512347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512347.2

ORF Insert Sequence:

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGT 
AAGGACGTTT TAAATAAATT AAAGGCATTA CATCCTTTAC CAGGCTTGAT ATTAGAATGG 
AGAAGAATCA CTAATGCTAT TACCAAAGTG GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT 
AATCCTTTTC TTGGAATGGA AAGAATCTAT CCTGTATCAC AGTCGCACAC TGCTACAGGA 
CGAATAACCT TTACAGAACC AAATATTCAG AATGTGCCAA GAGATTTTGA AATCAAAATG 
CCAACACTAG TAGGAGAAAG CCCACCTTCT CAAGCTGTAG GCAAAGGCCT ACTTCCCATG 
GGCAGAGGAA AATATAAGAA GGGTTTCAGC GTGAATCCTA GATGCCAGGC ACAGATGGAG 
GAGAGAGCTG CAGACAGAGG AATGCCATTT TCAATTAGCA TGCGACATGC CTTTGTGCCT 
TTCCCAGGTG GTTCAATACT GGCTGCTGAC TACTCTCAGC TTGAACTGAG GATCTTGGCT 
CATTTATCCC ATGATCGTCG TCTCATTCAA GTGTTAAACA CTGGAGCTGA TGTTTTCAGG 
AGCATTGCAG CAGAGTGGAA GATGATTGAG CCAGAGTCTG TTGGGGATGA TCTGAGGCAG 
CAGGCAAAAC AGATTTGCTA TGGGATCATT TATGGAATGG GAGCTAAATC TTTGGGAGAG 
CAGATGGGCA TTAAAGAAAA TGATGCTGCA TGCTATATTG ACTCCTTCAA ATCCAGATAC 
ACAGGGATTA ATCAATTCAT GACAGAGACA GTGAAGAATT GTAAAAGAGA CGGATTTGTT 
CAGACCATTT TGGGAAGGCG TAGATATTTG CCAGGAATCA AAGACAACAA CCCTTATCGT 
AAAGCTCACG CTGAGCGTCA AGCTATCAAC ACAATAGTCC AAGGATCAGC AGCTGATATT 
GTCAAAATAG CCACAGTTAA CATTCAGAAG CAATTAGAGA CCTTCCACTC AACCTTCAAA 
TCCCATGGTC ATCGAGAGGG TATGCTCCAA AGTGACCAAA CAGTAGGATT GTCACGAAAG 
AGAAAACTGC AAGGGATGTT CTGCCCAATC AGAGGAGGCT TCTTCATCCT TCAACTCCAT 
GATGAACTCC TATATGAAGT GGCAGAAGAA GATGTTGTTC AGGTAGCTCA GATTGTCAAG 
AATGAAATGG AAAGTGCTGT AAAACTGTCT GTGAAATTGA AAGTGAAAGT GAAAATAGGC 
GCCAGCTGGG GAGAGCTAAA GGACTTTGAT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_011512348.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510650.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X3, mRNA.

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CloneID	OHu64318
Clone ID Related Accession (Same CDS sequence)	XM_011512348.2
Accession Version	XM_011512348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA CCTCTCCATC AAGGCATTTC TCTTCATTGG ATATTGCAGT CTGCACAATT 
GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTGTTAGGA 
ATGGTGGTTG TGGATGAATT ACATATGCTG GGAGACTCTC ACCGAGGGTA TCTGCTGGAA 
CTTTTGCTGA CCAAGATTTG CTATATTACT CGGAAATCAG CATCTTGTCA GGCAGATCTA 
GCCAGTTCTC TGTCTAATGC TGTGCAAATC GTTGGCATGA GTGCTACCCT TCCTAATTTG 
GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCATA CCGACTTTCG CCCTGTACCG 
CTTTTGGAGT CAGTAAAAGT TGGAAATTCC ATATATGACT CTTCAATGAA ACTTGTGAGG 
GAATTTGAGC CCATGCTACA AGTGAAGGGA GATGAGGACC ATGTTGTTAG TTTATGTTAT 
GAGACGATTT GTGATAACCA TTCAGTATTA CTTTTTTGTC CATCAAAGAA ATGGTGTGAG 
AAGCTGGCAG ATATCATTGC TCGAGAGTTT TATAATCTAC ATCATCAAGC TGAGGGATTG 
GTGAAACCCT CTGAATGCCC ACCAGTAATT CTGGAACAAA AAGAACTCCT GGAAGTGATG 
GATCAGTTAA GACGTTTGCC TTCAGGACTG GACTCTGTAT TACAGAAAAC TGTACCATGG 
GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GGGATATCAT TGAAGGAGCC 
TTTCGTCAAG GTCTCATTCG GGTCTTGGCG GCAACTTCTA CTCTTTCTTC TGGGGTGAAT 
TTACCTGCAC GTCGTGTGAT TATTCGAACC CCTATTTTTG GTGGTCGACC TCTAGATATT 
CTTACTTATA AGCAGATGGT TGGCCGTGCT GGCAGGAAAG GAGTGGACAC AGTAGGCGAG 
AGTATCTTAA TTTGTAAGAA CTCTGAGAAA TCAAAAGGCA TAGCTCTCCT TCAGGGTTCT 
CTAAAGCCTG TTCGCAGCTG TCTGCAAAGA CGAGAAGGAG AAGAAGTAAC TGGCAGCATG 
ATACGAGCTA TTCTGGAGAT AATAGTTGGT GGAGTGGCAA GTACATCACA AGATATGCAT 
ACTTATGCTG CCTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA AGGAATTCAG 
AGAAATCAAG AGTCTGTTCA GCTTGGAGCG ATTGAGGCCT GTGTGATGTG GCTACTAGAA 
AATGAATTCA TCCAGAGTAC AGAAGCCAGT GATGGAACAG AAGGAAAGGT GTATCATCCA 
ACACATCTTG GTTCGGCCAC TCTTTCTTCT TCACTTTCTC CAGCTGATAC TTTAGATATT 
TTTGCTGACC TGCAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT TCATATTCTC 
TATCTGGTTA CACCTATGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT 
TTATGGGAGA AGTTGCCAAC TTCAATGAAA AGGGTGGCAG AGCTAGTGGG AGTTGAAGAG 
GGGTTCTTGG CCCGTTGTGT GAAAGGAAAA GTAGTAGCCA GAACTGAGAG ACAGCATCGA 
CAAATGGCCA TCCATAAAAG GTTTTTCACC AGTCTTGTGC TATTAGATTT AATCAGTGAA 
GTTCCCTTAA GGGAAATAAA TCAGAAATAT GGATGCAATC GTGGGCAGAT TCAATCTTTG 
CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTAT TTTCCAACCG TCTGGGCTGG 
CACAACATGG AACTACTACT TTCCCAATTT CAGAAGCGTC TTACGTTTGG CATCCAGAGG 
GAGCTGTGTG ACCTGGTTCG GGTATCCTTA CTAAATGCTC AGAGAGCCAG GGTTCTCTAT 
GCTTCTGGCT TTCATACTGT GGCAGACCTT GCTAGAGCAA ATATTGTGGA GGTGGAGGTG 
ATTCTGAAAA ATGCTGTGCC TTTCAAAAGT GCCCGGAAGG CAGTGGATGA GGAAGAGGAA 
GCAGTTGAAG AACGTCGCAA TATGCGAACT ATCTGGGTGA CTGGCAGAAA AGGTTTAACT 
GAAAGGGAAG CAGCAGCCCT TATAGTGGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA 
GTTGAAATGG GAGTGCAATG GAATCCATGT GCCCTGTTAC ATTCTAGTAC ATGCTCATTG 
ACTCATAGTG AGTCCGAAGT AAAGGAACAC ACATTTATAT CCCAAACTAA GAGTTCTTAT 
AAAAAATTAA CATCAAAGAA CAAAAGTAAC ACAATATTTA GTGATTCTTA TATTAAGCAT 
TCACCAAATA TAGTGCAAGA CTTAAATAAA AGTAGAGAGC ATACAAGTTC CTTTAATTGT 
AATTTCCAGA ATGGGAATCA AGAACATCAG ACATGTTCCA TTTTCAGAGC AAGAAAACGG 
GCCTCTTTAG ATATAAATAA AGAGAAGCCA GGAGCCTCTC AGAATGAGGG GAAAACAAGT 
GATAAGAAAG TTGTTCAGAC TTTTTCACAG AAAACAAAAA AGGCACCTTT GAATTTCAAT 
TCAGAAAAGA TGAGCAGAAG TTTTCGATCT TGGAAACGTA GAAAGCATCT AAAGCGATCT 
AGGGACAGCA GCCCCCTGAA AGACTCTGGA GCGTGTAGAA TCCATTTACA AGGACAGACT 
CTGTCTAATC CTAGTCTTTG TGAAGACCCG TTTACCTTAG ATGAGAAGAA AACGGAATTT 
AGAAATTCAG GGCCATTTGC TAAAAATGTA TCTTTGAGTG GTAAGGAAAA AGATAATAAA 
ACATCATTCC CATTACAAAT AAAGCAAAAT TGTTCATGGA ACATAACACT AACTAATGAT 
AATTTTGTGG AGCATATTGT CACAGGATCT CAGAGTAAAA ATGTGACTTG TCAGGCCACT 
AGTGTGGTTA GTGAAAAGGG CAGAGGAGTA GCTGTTGAGG CAGAAAAAAT AAATGAAGTG 
CTGATACAAA ATGGTTCAAA AAACCAGAAT GTTTATATGA AACACCATGA CATCCATCCA 
ATTAACCAGT ACCTGCGAAA GCAATCTCAT GAACAGACAA GCACTATTAC CAAACAGAAA 
AATATAATAG AGAGACAAAT GCCCTGTGAA GCAGTCAGTA GTTACATAAA TAGAGACTCA 
AATGTTACTA TCAATTGTGA AAGGATAAAG CTTAATACAG AGGAAAATAA ACCAAGTCAT 
TTTCAGGCAT TAGGAGATGA TATAAGCAGA ACTGTGATAC CCAGTGAAGT ACTTCCATCA 
GCTGGAGCAT TTAGCAAATC AGAAGGCCAG CATGAGAATT TTCTAAATAT TTCTAGACTA 
CAAGAAAAAA CAGGTACTTA TACAACAAAC AAAACTAAAA ATAATCATGT TTCTGACTTA 
GGTTTAGTCC TCTGTGATTT TGAAGATAGT TTCTATCTGG ATACTCAGTC AGAGAAAATA 
ATACAACAGA TGGCAACTGA AAATGCCAAA CTAGGAGCAA AGGACACCAA CCTGGCAGCA 
GGGATAATGC AGAAGAGCTT AGTCCAACAG AACTCAATGA ACTCTTTTCA GAAGGAGTGT 
CACATTCCTT TTCCTGCTGA ACAGCACCCT CTAGGAGCGA CTAAGATAGA TCATTTGGAC 
CTTAAGACTG TAGGTACTAT GAAACAAAGC AGTGATTCAC ATGGGGTTGA TATCCTGACT 
CCAGAAAGCC CGATTTTCCA TTCTCCAATA CTATTGGAGG AAAATGGTCT TTTTTTAAAA 
AAGAATGAAG TTTCTGTTAC TGATTCACAA TTAAATAGTT TTCTTCAAGG TTATCAAACA 
CAAGAAACTG TGAAACCAGT TATACTTCTG ATTCCTCAAA AGAGAACTCC CACTGGTGTA 
GAAGGAGAAT GTCTTCCAGT TCCTGAAACA AGTTTGAATA TGAGTGATAG TTTACTATTT 
GATAGCTTCA GTGATGACTA TCTAGTAAAA GAACAATTAC CTGATATGCA AATGAAAGAA 
CCCCTTCCTT CAGAAGTAAC ATCAAACCAT TTTAGTGATT CTCTGTGTCT ACAAGAAGAC 
CTAATTAAAA AATCAAATGT AAATGAGAAT CAAGATACCC ACCAGCAGTT GACTTGTTCC 
AATGATGAAT CTATTATATT TTCAGAAATG GATTCTGTTC AGATGGTTGA AGCTTTGGAC 
AATGTGGATA TATTTCCTGT CCAAGAGAAG AATCATACTG TAGTATCTCC TAGAGCATTA 
GAACTAAGTG ATCCAGTACT TGATGAGCAC CACCAAGGTG ATCAAGATGG AGGAGATCAA 
GATGAAAGGG CTGAAAAATC AAAATTAACT GGGACCAGGC AAAATCATTC ATTCATATGG 
TCAGGGGCAT CATTTGATCT AAGTCCAGGA CTGCAAAGGA TTTTAGATAA AGTATCCAGT 
CCTCTAGAAA ATGAAAAGCT AAAATCAATG ACTATAAACT TTTCCAGTTT GAATAGAAAA 
AATACAGAGT TAAATGAAGA ACAAGAAGTT ATTTCAAACT TGGAGACAAA ACAAGTGCAG 
GGAATTTCAT TTTCTTCTAA TAATGAAGTA AAAAGCAAGA TTGAGATGCT AGAAAACAAT 
GCCAATCATG ATGAAACCTC ATCCCTCTTA CCTCGTAAAG AAAGTAATAT AGTTGATGAT 
AATGGTCTCA TTCCTCCTAC ACCCATTCCA ACATCTGCTT CTAAGCTGAC ATTTCCAGGG 
ATTCTTGAAA CACCTGTAAA CCCTTGGAAA ACTAATAATG TTTTACAACC TGGTGAAAGT 
TATTTATTTG GCTCACCTTC AGATATTAAA AACCACGATT TAAGTCCAGG GAGTAGAAAT 
GGGTTCAAAG ACAACAGCCC TATTAGTGAC ACAAGCTTTT CACTTCAGTT ATCACAGGAT 
GGATTACAGT TAACTCCAGC CTCAAGCAGT TCAGAAAGTT TGTCCATAAT TGATGTAGCA 
AGTGACCAAA ATCTTTTCCA AACATTCATT AAGGAGTGGC GGTGCAAAAA GCGATTTTCC 
ATCTCACTGG CTTGTGAAAA GATTAGAAGT TTGACATCTT CTAAAACTGC TACTATTGGC 
AGTAGGTTTA AGCAAGCTAG CTCACCTCAG GAAATTCCTA TTAGAGATGA TGGATTTCCC 
ATTAAAGGTT GTGATGACAC CTTGGTGGTT GGACTGGCAG TATGCTGGGG TGGAAGGGAT 
GCCTATTATT TTTCACTGCA GAAGGAACAA AAGCATTCTG AAATTAGTGC CAGTTTGGTT 
CCACCTTCTT TAGATCCAAG CCTGACTTTG AAAGACAGGA TGTGGTACCT TCAATCTTGC 
TTGCGAAAGG AATCTGATAA AGAATGTTCT GTTGTCATCT ATGACTTCAT CCAGAGCTAT 
AAAATTCTTC TTCTTTCTTG TGGCATCTCC TTGGAGCAAA GTTATGAAGA TCCTAAGGTG 
GCATGCTGGT TACTAGATCC AGATTCTCAG GAGCCGACTC TTCATAGCAT AGTTACCAGT 
TTTCTTCCTC ATGAGCTTCC ACTCCTAGAA GGGATGGAGA CCAGCCAAGG GATTCAAAGC 
CTGGGGCTAA ATGCTGGCAG TGAGCATTCT GGGCGATACA GAGCATCTGT GGAGTCCATT 
CTCATCTTCA ACTCTATGAA TCAGCTCAAC TCTTTGTTGC AGAAGGAAAA CCTTCAAGAT 
GTTTTCCGTA AGGTGGAAAT GCCCTCTCAG TACTGCTTGG CCTTGCTAGA ACTAAATGGA 
ATTGGCTTTA GTACTGCAGA ATGTGAAAGT CAGAAACATA TAATGCAAGC CAAGCTGGAT 
GCAATTGAGA CCCAGGCCTA TCAACTAGCT GGCCACAGTT TTTCTTTCAC CAGTTCAGAT 
GACATCGCTG AGGTTTTATT TTTGGAATTG AAGTTGCCCC CAAATAGAGA GATGAAAAAC 
CAAGGCAGCA AGAAAACTCT GGGTTCTACC AGAAGAGGGA TTGACAATGG ACGCAAGCTA 
AGGCTGGGAA GACAGTTCAG CACTAGTAAG GACGTTTTAA ATAAATTAAA GGCATTACAT 
CCTTTACCAG GCTTGATATT AGAATGGAGA AGAATCACTA ATGCTATTAC CAAAGTGGTC 
TTTCCCCTTC AGCGGGAAAA GTGTCTTAAT CCTTTTCTTG GAATGGAAAG AATCTATCCT 
GTATCACAGT CGCACACTGC TACAGGACGA ATAACCTTTA CAGAACCAAA TATTCAGAAT 
GTGCCAAGAG ATTTTGAAAT CAAAATGCCA ACACTAGTAG GAGAAAGCCC ACCTTCTCAA 
GCTGTAGGCA AAGGCCTACT TCCCATGGGC AGAGGAAAAT ATAAGAAGGG TTTCAGCGTG 
AATCCTAGAT GCCAGGCACA GATGGAGGAG AGAGCTGCAG ACAGAGGAAT GCCATTTTCA 
ATTAGCATGC GACATGCCTT TGTGCCTTTC CCAGGTGGTT CAATACTGGC TGCTGACTAC 
TCTCAGCTTG AACTGAGGAT CTTGGCTCAT TTATCCCATG ATCGTCGTCT CATTCAAGTG 
TTAAACACTG GAGCTGATGT TTTCAGGAGC ATTGCAGCAG AGTGGAAGAT GATTGAGCCA 
GAGTCTGTTG GGGATGATCT GAGGCAGCAG GCAAAACAGA TTTGCTATGG GATCATTTAT 
GGAATGGGAG CTAAATCTTT GGGAGAGCAG ATGGGCATTA AAGAAAATGA TGCTGCATGC 
TATATTGACT CCTTCAAATC CAGATACACA GGGATTAATC AATTCATGAC AGAGACAGTG 
AAGAATTGTA AAAGAGACGG ATTTGTTCAG ACCATTTTGG GAAGGCGTAG ATATTTGCCA 
GGAATCAAAG ACAACAACCC TTATCGTAAA GCTCACGCTG AGCGTCAAGC TATCAACACA 
ATAGTCCAAG GATCAGCAGC TGATATTGTC AAAATAGCCA CAGTTAACAT TCAGAAGCAA 
TTAGAGACCT TCCACTCAAC CTTCAAATCC CATGGTCATC GAGAGGGTAT GCTCCAAAGT 
GACCAAACAG TAGGATTGTC ACGAAAGAGA AAACTGCAAG GGATGTTCTG CCCAATCAGA 
GGAGGCTTCT TCATCCTTCA ACTCCATGAT GAACTCCTAT ATGAAGTGGC AGAAGAAGAT 
GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT GAAATGGAAA GTGCTGTAAA ACTGTCTGTG 
AAATTGAAAG TGAAAGTGAA AATAGGCGCC AGCTGGGGAG AGCTAAAGGA CTTTGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510650.1
CDS	500..7762
Translation	MGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTVGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	XM_011512348.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512348.2

ORF Insert Sequence:

ATGGGCAGCA CCTCTCCATC AAGGCATTTC TCTTCATTGG ATATTGCAGT CTGCACAATT 
GAGAGAGCCA ATGGTCTGAT CAATCGCCTC ATAGAGGAAA ATAAGATGGA TCTGTTAGGA 
ATGGTGGTTG TGGATGAATT ACATATGCTG GGAGACTCTC ACCGAGGGTA TCTGCTGGAA 
CTTTTGCTGA CCAAGATTTG CTATATTACT CGGAAATCAG CATCTTGTCA GGCAGATCTA 
GCCAGTTCTC TGTCTAATGC TGTGCAAATC GTTGGCATGA GTGCTACCCT TCCTAATTTG 
GAGCTTGTGG CTTCCTGGTT GAATGCTGAA CTCTACCATA CCGACTTTCG CCCTGTACCG 
CTTTTGGAGT CAGTAAAAGT TGGAAATTCC ATATATGACT CTTCAATGAA ACTTGTGAGG 
GAATTTGAGC CCATGCTACA AGTGAAGGGA GATGAGGACC ATGTTGTTAG TTTATGTTAT 
GAGACGATTT GTGATAACCA TTCAGTATTA CTTTTTTGTC CATCAAAGAA ATGGTGTGAG 
AAGCTGGCAG ATATCATTGC TCGAGAGTTT TATAATCTAC ATCATCAAGC TGAGGGATTG 
GTGAAACCCT CTGAATGCCC ACCAGTAATT CTGGAACAAA AAGAACTCCT GGAAGTGATG 
GATCAGTTAA GACGTTTGCC TTCAGGACTG GACTCTGTAT TACAGAAAAC TGTACCATGG 
GGAGTAGCAT TTCATCATGC AGGTCTTACT TTTGAGGAGA GGGATATCAT TGAAGGAGCC 
TTTCGTCAAG GTCTCATTCG GGTCTTGGCG GCAACTTCTA CTCTTTCTTC TGGGGTGAAT 
TTACCTGCAC GTCGTGTGAT TATTCGAACC CCTATTTTTG GTGGTCGACC TCTAGATATT 
CTTACTTATA AGCAGATGGT TGGCCGTGCT GGCAGGAAAG GAGTGGACAC AGTAGGCGAG 
AGTATCTTAA TTTGTAAGAA CTCTGAGAAA TCAAAAGGCA TAGCTCTCCT TCAGGGTTCT 
CTAAAGCCTG TTCGCAGCTG TCTGCAAAGA CGAGAAGGAG AAGAAGTAAC TGGCAGCATG 
ATACGAGCTA TTCTGGAGAT AATAGTTGGT GGAGTGGCAA GTACATCACA AGATATGCAT 
ACTTATGCTG CCTGCACATT TTTGGCTGCA AGTATGAAAG AAGGGAAGCA AGGAATTCAG 
AGAAATCAAG AGTCTGTTCA GCTTGGAGCG ATTGAGGCCT GTGTGATGTG GCTACTAGAA 
AATGAATTCA TCCAGAGTAC AGAAGCCAGT GATGGAACAG AAGGAAAGGT GTATCATCCA 
ACACATCTTG GTTCGGCCAC TCTTTCTTCT TCACTTTCTC CAGCTGATAC TTTAGATATT 
TTTGCTGACC TGCAAAGAGC AATGAAGGGC TTTGTTTTAG AGAATGATCT TCATATTCTC 
TATCTGGTTA CACCTATGTT TGAGGATTGG ACTACTATTG ATTGGTATCG ATTTTTCTGT 
TTATGGGAGA AGTTGCCAAC TTCAATGAAA AGGGTGGCAG AGCTAGTGGG AGTTGAAGAG 
GGGTTCTTGG CCCGTTGTGT GAAAGGAAAA GTAGTAGCCA GAACTGAGAG ACAGCATCGA 
CAAATGGCCA TCCATAAAAG GTTTTTCACC AGTCTTGTGC TATTAGATTT AATCAGTGAA 
GTTCCCTTAA GGGAAATAAA TCAGAAATAT GGATGCAATC GTGGGCAGAT TCAATCTTTG 
CAACAGTCAG CTGCTGTTTA TGCAGGGATG ATTACAGTAT TTTCCAACCG TCTGGGCTGG 
CACAACATGG AACTACTACT TTCCCAATTT CAGAAGCGTC TTACGTTTGG CATCCAGAGG 
GAGCTGTGTG ACCTGGTTCG GGTATCCTTA CTAAATGCTC AGAGAGCCAG GGTTCTCTAT 
GCTTCTGGCT TTCATACTGT GGCAGACCTT GCTAGAGCAA ATATTGTGGA GGTGGAGGTG 
ATTCTGAAAA ATGCTGTGCC TTTCAAAAGT GCCCGGAAGG CAGTGGATGA GGAAGAGGAA 
GCAGTTGAAG AACGTCGCAA TATGCGAACT ATCTGGGTGA CTGGCAGAAA AGGTTTAACT 
GAAAGGGAAG CAGCAGCCCT TATAGTGGAA GAAGCCAGAA TGATTCTGCA GCAGGACTTA 
GTTGAAATGG GAGTGCAATG GAATCCATGT GCCCTGTTAC ATTCTAGTAC ATGCTCATTG 
ACTCATAGTG AGTCCGAAGT AAAGGAACAC ACATTTATAT CCCAAACTAA GAGTTCTTAT 
AAAAAATTAA CATCAAAGAA CAAAAGTAAC ACAATATTTA GTGATTCTTA TATTAAGCAT 
TCACCAAATA TAGTGCAAGA CTTAAATAAA AGTAGAGAGC ATACAAGTTC CTTTAATTGT 
AATTTCCAGA ATGGGAATCA AGAACATCAG ACATGTTCCA TTTTCAGAGC AAGAAAACGG 
GCCTCTTTAG ATATAAATAA AGAGAAGCCA GGAGCCTCTC AGAATGAGGG GAAAACAAGT 
GATAAGAAAG TTGTTCAGAC TTTTTCACAG AAAACAAAAA AGGCACCTTT GAATTTCAAT 
TCAGAAAAGA TGAGCAGAAG TTTTCGATCT TGGAAACGTA GAAAGCATCT AAAGCGATCT 
AGGGACAGCA GCCCCCTGAA AGACTCTGGA GCGTGTAGAA TCCATTTACA AGGACAGACT 
CTGTCTAATC CTAGTCTTTG TGAAGACCCG TTTACCTTAG ATGAGAAGAA AACGGAATTT 
AGAAATTCAG GGCCATTTGC TAAAAATGTA TCTTTGAGTG GTAAGGAAAA AGATAATAAA 
ACATCATTCC CATTACAAAT AAAGCAAAAT TGTTCATGGA ACATAACACT AACTAATGAT 
AATTTTGTGG AGCATATTGT CACAGGATCT CAGAGTAAAA ATGTGACTTG TCAGGCCACT 
AGTGTGGTTA GTGAAAAGGG CAGAGGAGTA GCTGTTGAGG CAGAAAAAAT AAATGAAGTG 
CTGATACAAA ATGGTTCAAA AAACCAGAAT GTTTATATGA AACACCATGA CATCCATCCA 
ATTAACCAGT ACCTGCGAAA GCAATCTCAT GAACAGACAA GCACTATTAC CAAACAGAAA 
AATATAATAG AGAGACAAAT GCCCTGTGAA GCAGTCAGTA GTTACATAAA TAGAGACTCA 
AATGTTACTA TCAATTGTGA AAGGATAAAG CTTAATACAG AGGAAAATAA ACCAAGTCAT 
TTTCAGGCAT TAGGAGATGA TATAAGCAGA ACTGTGATAC CCAGTGAAGT ACTTCCATCA 
GCTGGAGCAT TTAGCAAATC AGAAGGCCAG CATGAGAATT TTCTAAATAT TTCTAGACTA 
CAAGAAAAAA CAGGTACTTA TACAACAAAC AAAACTAAAA ATAATCATGT TTCTGACTTA 
GGTTTAGTCC TCTGTGATTT TGAAGATAGT TTCTATCTGG ATACTCAGTC AGAGAAAATA 
ATACAACAGA TGGCAACTGA AAATGCCAAA CTAGGAGCAA AGGACACCAA CCTGGCAGCA 
GGGATAATGC AGAAGAGCTT AGTCCAACAG AACTCAATGA ACTCTTTTCA GAAGGAGTGT 
CACATTCCTT TTCCTGCTGA ACAGCACCCT CTAGGAGCGA CTAAGATAGA TCATTTGGAC 
CTTAAGACTG TAGGTACTAT GAAACAAAGC AGTGATTCAC ATGGGGTTGA TATCCTGACT 
CCAGAAAGCC CGATTTTCCA TTCTCCAATA CTATTGGAGG AAAATGGTCT TTTTTTAAAA 
AAGAATGAAG TTTCTGTTAC TGATTCACAA TTAAATAGTT TTCTTCAAGG TTATCAAACA 
CAAGAAACTG TGAAACCAGT TATACTTCTG ATTCCTCAAA AGAGAACTCC CACTGGTGTA 
GAAGGAGAAT GTCTTCCAGT TCCTGAAACA AGTTTGAATA TGAGTGATAG TTTACTATTT 
GATAGCTTCA GTGATGACTA TCTAGTAAAA GAACAATTAC CTGATATGCA AATGAAAGAA 
CCCCTTCCTT CAGAAGTAAC ATCAAACCAT TTTAGTGATT CTCTGTGTCT ACAAGAAGAC 
CTAATTAAAA AATCAAATGT AAATGAGAAT CAAGATACCC ACCAGCAGTT GACTTGTTCC 
AATGATGAAT CTATTATATT TTCAGAAATG GATTCTGTTC AGATGGTTGA AGCTTTGGAC 
AATGTGGATA TATTTCCTGT CCAAGAGAAG AATCATACTG TAGTATCTCC TAGAGCATTA 
GAACTAAGTG ATCCAGTACT TGATGAGCAC CACCAAGGTG ATCAAGATGG AGGAGATCAA 
GATGAAAGGG CTGAAAAATC AAAATTAACT GGGACCAGGC AAAATCATTC ATTCATATGG 
TCAGGGGCAT CATTTGATCT AAGTCCAGGA CTGCAAAGGA TTTTAGATAA AGTATCCAGT 
CCTCTAGAAA ATGAAAAGCT AAAATCAATG ACTATAAACT TTTCCAGTTT GAATAGAAAA 
AATACAGAGT TAAATGAAGA ACAAGAAGTT ATTTCAAACT TGGAGACAAA ACAAGTGCAG 
GGAATTTCAT TTTCTTCTAA TAATGAAGTA AAAAGCAAGA TTGAGATGCT AGAAAACAAT 
GCCAATCATG ATGAAACCTC ATCCCTCTTA CCTCGTAAAG AAAGTAATAT AGTTGATGAT 
AATGGTCTCA TTCCTCCTAC ACCCATTCCA ACATCTGCTT CTAAGCTGAC ATTTCCAGGG 
ATTCTTGAAA CACCTGTAAA CCCTTGGAAA ACTAATAATG TTTTACAACC TGGTGAAAGT 
TATTTATTTG GCTCACCTTC AGATATTAAA AACCACGATT TAAGTCCAGG GAGTAGAAAT 
GGGTTCAAAG ACAACAGCCC TATTAGTGAC ACAAGCTTTT CACTTCAGTT ATCACAGGAT 
GGATTACAGT TAACTCCAGC CTCAAGCAGT TCAGAAAGTT TGTCCATAAT TGATGTAGCA 
AGTGACCAAA ATCTTTTCCA AACATTCATT AAGGAGTGGC GGTGCAAAAA GCGATTTTCC 
ATCTCACTGG CTTGTGAAAA GATTAGAAGT TTGACATCTT CTAAAACTGC TACTATTGGC 
AGTAGGTTTA AGCAAGCTAG CTCACCTCAG GAAATTCCTA TTAGAGATGA TGGATTTCCC 
ATTAAAGGTT GTGATGACAC CTTGGTGGTT GGACTGGCAG TATGCTGGGG TGGAAGGGAT 
GCCTATTATT TTTCACTGCA GAAGGAACAA AAGCATTCTG AAATTAGTGC CAGTTTGGTT 
CCACCTTCTT TAGATCCAAG CCTGACTTTG AAAGACAGGA TGTGGTACCT TCAATCTTGC 
TTGCGAAAGG AATCTGATAA AGAATGTTCT GTTGTCATCT ATGACTTCAT CCAGAGCTAT 
AAAATTCTTC TTCTTTCTTG TGGCATCTCC TTGGAGCAAA GTTATGAAGA TCCTAAGGTG 
GCATGCTGGT TACTAGATCC AGATTCTCAG GAGCCGACTC TTCATAGCAT AGTTACCAGT 
TTTCTTCCTC ATGAGCTTCC ACTCCTAGAA GGGATGGAGA CCAGCCAAGG GATTCAAAGC 
CTGGGGCTAA ATGCTGGCAG TGAGCATTCT GGGCGATACA GAGCATCTGT GGAGTCCATT 
CTCATCTTCA ACTCTATGAA TCAGCTCAAC TCTTTGTTGC AGAAGGAAAA CCTTCAAGAT 
GTTTTCCGTA AGGTGGAAAT GCCCTCTCAG TACTGCTTGG CCTTGCTAGA ACTAAATGGA 
ATTGGCTTTA GTACTGCAGA ATGTGAAAGT CAGAAACATA TAATGCAAGC CAAGCTGGAT 
GCAATTGAGA CCCAGGCCTA TCAACTAGCT GGCCACAGTT TTTCTTTCAC CAGTTCAGAT 
GACATCGCTG AGGTTTTATT TTTGGAATTG AAGTTGCCCC CAAATAGAGA GATGAAAAAC 
CAAGGCAGCA AGAAAACTCT GGGTTCTACC AGAAGAGGGA TTGACAATGG ACGCAAGCTA 
AGGCTGGGAA GACAGTTCAG CACTAGTAAG GACGTTTTAA ATAAATTAAA GGCATTACAT 
CCTTTACCAG GCTTGATATT AGAATGGAGA AGAATCACTA ATGCTATTAC CAAAGTGGTC 
TTTCCCCTTC AGCGGGAAAA GTGTCTTAAT CCTTTTCTTG GAATGGAAAG AATCTATCCT 
GTATCACAGT CGCACACTGC TACAGGACGA ATAACCTTTA CAGAACCAAA TATTCAGAAT 
GTGCCAAGAG ATTTTGAAAT CAAAATGCCA ACACTAGTAG GAGAAAGCCC ACCTTCTCAA 
GCTGTAGGCA AAGGCCTACT TCCCATGGGC AGAGGAAAAT ATAAGAAGGG TTTCAGCGTG 
AATCCTAGAT GCCAGGCACA GATGGAGGAG AGAGCTGCAG ACAGAGGAAT GCCATTTTCA 
ATTAGCATGC GACATGCCTT TGTGCCTTTC CCAGGTGGTT CAATACTGGC TGCTGACTAC 
TCTCAGCTTG AACTGAGGAT CTTGGCTCAT TTATCCCATG ATCGTCGTCT CATTCAAGTG 
TTAAACACTG GAGCTGATGT TTTCAGGAGC ATTGCAGCAG AGTGGAAGAT GATTGAGCCA 
GAGTCTGTTG GGGATGATCT GAGGCAGCAG GCAAAACAGA TTTGCTATGG GATCATTTAT 
GGAATGGGAG CTAAATCTTT GGGAGAGCAG ATGGGCATTA AAGAAAATGA TGCTGCATGC 
TATATTGACT CCTTCAAATC CAGATACACA GGGATTAATC AATTCATGAC AGAGACAGTG 
AAGAATTGTA AAAGAGACGG ATTTGTTCAG ACCATTTTGG GAAGGCGTAG ATATTTGCCA 
GGAATCAAAG ACAACAACCC TTATCGTAAA GCTCACGCTG AGCGTCAAGC TATCAACACA 
ATAGTCCAAG GATCAGCAGC TGATATTGTC AAAATAGCCA CAGTTAACAT TCAGAAGCAA 
TTAGAGACCT TCCACTCAAC CTTCAAATCC CATGGTCATC GAGAGGGTAT GCTCCAAAGT 
GACCAAACAG TAGGATTGTC ACGAAAGAGA AAACTGCAAG GGATGTTCTG CCCAATCAGA 
GGAGGCTTCT TCATCCTTCA ACTCCATGAT GAACTCCTAT ATGAAGTGGC AGAAGAAGAT 
GTTGTTCAGG TAGCTCAGAT TGTCAAGAAT GAAATGGAAA GTGCTGTAAA ACTGTCTGTG 
AAATTGAAAG TGAAAGTGAA AATAGGCGCC AGCTGGGGAG AGCTAAAGGA CTTTGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_011512352.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510654.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X6, mRNA.

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CloneID	OHu64321
Clone ID Related Accession (Same CDS sequence)	XM_011512352.2
Accession Version	XM_011512352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGA 
CGTTTTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510654.1
CDS	118..6669
Translation	MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTRRFK

Target ORF information:

RefSeq Version	XM_011512352.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512352.2

ORF Insert Sequence:

ATGAATCTTC TGCGTCGGAG TGGGAAACGG CGGCGTTCAG AATCAGGCTC AGATTCGTTC 
TCGGGAAGCG GCGGTGACAG CAGTGCCAGC CCCCAGTTCC TCTCCGGGTC CGTGCTGAGC 
CCGCCGCCCG GCCTTGGTCG CTGCCTGAAG GCCGCAGCTG CAGGAGAATG CAAGCCTACA 
GTTCCTGACT ACGAAAGAGA CAAGCTACTA TTGGCAAACT GGGGACTTCC TAAAGCAGTT 
CTGGAAAAAT ACCACAGTTT TGGTGTAAAA AAGATGTTTG AATGGCAGGC AGAGTGCCTT 
TTGCTTGGAC AAGTCCTGGA AGGAAAGAAT TTAGTTTATT CAGCTCCTAC AAGTGCTGGG 
AAGACTCTTG TGGCAGAATT ACTTATTTTG AAGCGGGTTT TGGAAATGCG GAAGAAAGCT 
TTGTTTATTC TTCCCTTTGT TTCTGTGGCT AAAGAGAAGA AATACTACCT CCAGAGTCTG 
TTTCAGGAAG TAGGAATAAA AGTAGACGGT TATATGGGCA GCACCTCTCC ATCAAGGCAT 
TTCTCTTCAT TGGATATTGC AGTCTGCACA ATTGAGAGAG CCAATGGTCT GATCAATCGC 
CTCATAGAGG AAAATAAGAT GGATCTGTTA GGAATGGTGG TTGTGGATGA ATTACATATG 
CTGGGAGACT CTCACCGAGG GTATCTGCTG GAACTTTTGC TGACCAAGAT TTGCTATATT 
ACTCGGAAAT CAGCATCTTG TCAGGCAGAT CTAGCCAGTT CTCTGTCTAA TGCTGTGCAA 
ATCGTTGGCA TGAGTGCTAC CCTTCCTAAT TTGGAGCTTG TGGCTTCCTG GTTGAATGCT 
GAACTCTACC ATACCGACTT TCGCCCTGTA CCGCTTTTGG AGTCAGTAAA AGTTGGAAAT 
TCCATATATG ACTCTTCAAT GAAACTTGTG AGGGAATTTG AGCCCATGCT ACAAGTGAAG 
GGAGATGAGG ACCATGTTGT TAGTTTATGT TATGAGACGA TTTGTGATAA CCATTCAGTA 
TTACTTTTTT GTCCATCAAA GAAATGGTGT GAGAAGCTGG CAGATATCAT TGCTCGAGAG 
TTTTATAATC TACATCATCA AGCTGAGGGA TTGGTGAAAC CCTCTGAATG CCCACCAGTA 
ATTCTGGAAC AAAAAGAACT CCTGGAAGTG ATGGATCAGT TAAGACGTTT GCCTTCAGGA 
CTGGACTCTG TATTACAGAA AACTGTACCA TGGGGAGTAG CATTTCATCA TGCAGGTCTT 
ACTTTTGAGG AGAGGGATAT CATTGAAGGA GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG 
GCGGCAACTT CTACTCTTTC TTCTGGGGTG AATTTACCTG CACGTCGTGT GATTATTCGA 
ACCCCTATTT TTGGTGGTCG ACCTCTAGAT ATTCTTACTT ATAAGCAGAT GGTTGGCCGT 
GCTGGCAGGA AAGGAGTGGA CACAGTAGGC GAGAGTATCT TAATTTGTAA GAACTCTGAG 
AAATCAAAAG GCATAGCTCT CCTTCAGGGT TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA 
AGACGAGAAG GAGAAGAAGT AACTGGCAGC ATGATACGAG CTATTCTGGA GATAATAGTT 
GGTGGAGTGG CAAGTACATC ACAAGATATG CATACTTATG CTGCCTGCAC ATTTTTGGCT 
GCAAGTATGA AAGAAGGGAA GCAAGGAATT CAGAGAAATC AAGAGTCTGT TCAGCTTGGA 
GCGATTGAGG CCTGTGTGAT GTGGCTACTA GAAAATGAAT TCATCCAGAG TACAGAAGCC 
AGTGATGGAA CAGAAGGAAA GGTGTATCAT CCAACACATC TTGGTTCGGC CACTCTTTCT 
TCTTCACTTT CTCCAGCTGA TACTTTAGAT ATTTTTGCTG ACCTGCAAAG AGCAATGAAG 
GGCTTTGTTT TAGAGAATGA TCTTCATATT CTCTATCTGG TTACACCTAT GTTTGAGGAT 
TGGACTACTA TTGATTGGTA TCGATTTTTC TGTTTATGGG AGAAGTTGCC AACTTCAATG 
AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA 
AAAGTAGTAG CCAGAACTGA GAGACAGCAT CGACAAATGG CCATCCATAA AAGGTTTTTC 
ACCAGTCTTG TGCTATTAGA TTTAATCAGT GAAGTTCCCT TAAGGGAAAT AAATCAGAAA 
TATGGATGCA ATCGTGGGCA GATTCAATCT TTGCAACAGT CAGCTGCTGT TTATGCAGGG 
ATGATTACAG TATTTTCCAA CCGTCTGGGC TGGCACAACA TGGAACTACT ACTTTCCCAA 
TTTCAGAAGC GTCTTACGTT TGGCATCCAG AGGGAGCTGT GTGACCTGGT TCGGGTATCC 
TTACTAAATG CTCAGAGAGC CAGGGTTCTC TATGCTTCTG GCTTTCATAC TGTGGCAGAC 
CTTGCTAGAG CAAATATTGT GGAGGTGGAG GTGATTCTGA AAAATGCTGT GCCTTTCAAA 
AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG GAAGCAGTTG AAGAACGTCG CAATATGCGA 
ACTATCTGGG TGACTGGCAG AAAAGGTTTA ACTGAAAGGG AAGCAGCAGC CCTTATAGTG 
GAAGAAGCCA GAATGATTCT GCAGCAGGAC TTAGTTGAAA TGGGAGTGCA ATGGAATCCA 
TGTGCCCTGT TACATTCTAG TACATGCTCA TTGACTCATA GTGAGTCCGA AGTAAAGGAA 
CACACATTTA TATCCCAAAC TAAGAGTTCT TATAAAAAAT TAACATCAAA GAACAAAAGT 
AACACAATAT TTAGTGATTC TTATATTAAG CATTCACCAA ATATAGTGCA AGACTTAAAT 
AAAAGTAGAG AGCATACAAG TTCCTTTAAT TGTAATTTCC AGAATGGGAA TCAAGAACAT 
CAGACATGTT CCATTTTCAG AGCAAGAAAA CGGGCCTCTT TAGATATAAA TAAAGAGAAG 
CCAGGAGCCT CTCAGAATGA GGGGAAAACA AGTGATAAGA AAGTTGTTCA GACTTTTTCA 
CAGAAAACAA AAAAGGCACC TTTGAATTTC AATTCAGAAA AGATGAGCAG AAGTTTTCGA 
TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA TCTAGGGACA GCAGCCCCCT GAAAGACTCT 
GGAGCGTGTA GAATCCATTT ACAAGGACAG ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC 
CCGTTTACCT TAGATGAGAA GAAAACGGAA TTTAGAAATT CAGGGCCATT TGCTAAAAAT 
GTATCTTTGA GTGGTAAGGA AAAAGATAAT AAAACATCAT TCCCATTACA AATAAAGCAA 
AATTGTTCAT GGAACATAAC ACTAACTAAT GATAATTTTG TGGAGCATAT TGTCACAGGA 
TCTCAGAGTA AAAATGTGAC TTGTCAGGCC ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA 
GTAGCTGTTG AGGCAGAAAA AATAAATGAA GTGCTGATAC AAAATGGTTC AAAAAACCAG 
AATGTTTATA TGAAACACCA TGACATCCAT CCAATTAACC AGTACCTGCG AAAGCAATCT 
CATGAACAGA CAAGCACTAT TACCAAACAG AAAAATATAA TAGAGAGACA AATGCCCTGT 
GAAGCAGTCA GTAGTTACAT AAATAGAGAC TCAAATGTTA CTATCAATTG TGAAAGGATA 
AAGCTTAATA CAGAGGAAAA TAAACCAAGT CATTTTCAGG CATTAGGAGA TGATATAAGC 
AGAACTGTGA TACCCAGTGA AGTACTTCCA TCAGCTGGAG CATTTAGCAA ATCAGAAGGC 
CAGCATGAGA ATTTTCTAAA TATTTCTAGA CTACAAGAAA AAACAGGTAC TTATACAACA 
AACAAAACTA AAAATAATCA TGTTTCTGAC TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT 
AGTTTCTATC TGGATACTCA GTCAGAGAAA ATAATACAAC AGATGGCAAC TGAAAATGCC 
AAACTAGGAG CAAAGGACAC CAACCTGGCA GCAGGGATAA TGCAGAAGAG CTTAGTCCAA 
CAGAACTCAA TGAACTCTTT TCAGAAGGAG TGTCACATTC CTTTTCCTGC TGAACAGCAC 
CCTCTAGGAG CGACTAAGAT AGATCATTTG GACCTTAAGA CTGTAGGTAC TATGAAACAA 
AGCAGTGATT CACATGGGGT TGATATCCTG ACTCCAGAAA GCCCGATTTT CCATTCTCCA 
ATACTATTGG AGGAAAATGG TCTTTTTTTA AAAAAGAATG AAGTTTCTGT TACTGATTCA 
CAATTAAATA GTTTTCTTCA AGGTTATCAA ACACAAGAAA CTGTGAAACC AGTTATACTT 
CTGATTCCTC AAAAGAGAAC TCCCACTGGT GTAGAAGGAG AATGTCTTCC AGTTCCTGAA 
ACAAGTTTGA ATATGAGTGA TAGTTTACTA TTTGATAGCT TCAGTGATGA CTATCTAGTA 
AAAGAACAAT TACCTGATAT GCAAATGAAA GAACCCCTTC CTTCAGAAGT AACATCAAAC 
CATTTTAGTG ATTCTCTGTG TCTACAAGAA GACCTAATTA AAAAATCAAA TGTAAATGAG 
AATCAAGATA CCCACCAGCA GTTGACTTGT TCCAATGATG AATCTATTAT ATTTTCAGAA 
ATGGATTCTG TTCAGATGGT TGAAGCTTTG GACAATGTGG ATATATTTCC TGTCCAAGAG 
AAGAATCATA CTGTAGTATC TCCTAGAGCA TTAGAACTAA GTGATCCAGT ACTTGATGAG 
CACCACCAAG GTGATCAAGA TGGAGGAGAT CAAGATGAAA GGGCTGAAAA ATCAAAATTA 
ACTGGGACCA GGCAAAATCA TTCATTCATA TGGTCAGGGG CATCATTTGA TCTAAGTCCA 
GGACTGCAAA GGATTTTAGA TAAAGTATCC AGTCCTCTAG AAAATGAAAA GCTAAAATCA 
ATGACTATAA ACTTTTCCAG TTTGAATAGA AAAAATACAG AGTTAAATGA AGAACAAGAA 
GTTATTTCAA ACTTGGAGAC AAAACAAGTG CAGGGAATTT CATTTTCTTC TAATAATGAA 
GTAAAAAGCA AGATTGAGAT GCTAGAAAAC AATGCCAATC ATGATGAAAC CTCATCCCTC 
TTACCTCGTA AAGAAAGTAA TATAGTTGAT GATAATGGTC TCATTCCTCC TACACCCATT 
CCAACATCTG CTTCTAAGCT GACATTTCCA GGGATTCTTG AAACACCTGT AAACCCTTGG 
AAAACTAATA ATGTTTTACA ACCTGGTGAA AGTTATTTAT TTGGCTCACC TTCAGATATT 
AAAAACCACG ATTTAAGTCC AGGGAGTAGA AATGGGTTCA AAGACAACAG CCCTATTAGT 
GACACAAGCT TTTCACTTCA GTTATCACAG GATGGATTAC AGTTAACTCC AGCCTCAAGC 
AGTTCAGAAA GTTTGTCCAT AATTGATGTA GCAAGTGACC AAAATCTTTT CCAAACATTC 
ATTAAGGAGT GGCGGTGCAA AAAGCGATTT TCCATCTCAC TGGCTTGTGA AAAGATTAGA 
AGTTTGACAT CTTCTAAAAC TGCTACTATT GGCAGTAGGT TTAAGCAAGC TAGCTCACCT 
CAGGAAATTC CTATTAGAGA TGATGGATTT CCCATTAAAG GTTGTGATGA CACCTTGGTG 
GTTGGACTGG CAGTATGCTG GGGTGGAAGG GATGCCTATT ATTTTTCACT GCAGAAGGAA 
CAAAAGCATT CTGAAATTAG TGCCAGTTTG GTTCCACCTT CTTTAGATCC AAGCCTGACT 
TTGAAAGACA GGATGTGGTA CCTTCAATCT TGCTTGCGAA AGGAATCTGA TAAAGAATGT 
TCTGTTGTCA TCTATGACTT CATCCAGAGC TATAAAATTC TTCTTCTTTC TTGTGGCATC 
TCCTTGGAGC AAAGTTATGA AGATCCTAAG GTGGCATGCT GGTTACTAGA TCCAGATTCT 
CAGGAGCCGA CTCTTCATAG CATAGTTACC AGTTTTCTTC CTCATGAGCT TCCACTCCTA 
GAAGGGATGG AGACCAGCCA AGGGATTCAA AGCCTGGGGC TAAATGCTGG CAGTGAGCAT 
TCTGGGCGAT ACAGAGCATC TGTGGAGTCC ATTCTCATCT TCAACTCTAT GAATCAGCTC 
AACTCTTTGT TGCAGAAGGA AAACCTTCAA GATGTTTTCC GTAAGGTGGA AATGCCCTCT 
CAGTACTGCT TGGCCTTGCT AGAACTAAAT GGAATTGGCT TTAGTACTGC AGAATGTGAA 
AGTCAGAAAC ATATAATGCA AGCCAAGCTG GATGCAATTG AGACCCAGGC CTATCAACTA 
GCTGGCCACA GTTTTTCTTT CACCAGTTCA GATGACATCG CTGAGGTTTT ATTTTTGGAA 
TTGAAGTTGC CCCCAAATAG AGAGATGAAA AACCAAGGCA GCAAGAAAAC TCTGGGTTCT 
ACCAGAAGAG GGATTGACAA TGGACGCAAG CTAAGGCTGG GAAGACAGTT CAGCACTAGA 
CGTTTTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLQ ( XM_017005565.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861054.1 Homo sapiens DNA polymerase theta (POLQ), transcript variant X5, mRNA.

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CloneID	OHu93745
Clone ID Related Accession (Same CDS sequence)	XM_017005565.1
Accession Version	XM_017005565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	DNA polymerase theta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGT TAAGACGTTT GCCTTCAGGA CTGGACTCTG TATTACAGAA AACTGTACCA 
TGGGGAGTAG CATTTCATCA TGCAGGTCTT ACTTTTGAGG AGAGGGATAT CATTGAAGGA 
GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG GCGGCAACTT CTACTCTTTC TTCTGGGGTG 
AATTTACCTG CACGTCGTGT GATTATTCGA ACCCCTATTT TTGGTGGTCG ACCTCTAGAT 
ATTCTTACTT ATAAGCAGAT GGTTGGCCGT GCTGGCAGGA AAGGAGTGGA CACAGTAGGC 
GAGAGTATCT TAATTTGTAA GAACTCTGAG AAATCAAAAG GCATAGCTCT CCTTCAGGGT 
TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA AGACGAGAAG GAGAAGAAGT AACTGGCAGC 
ATGATACGAG CTATTCTGGA GATAATAGTT GGTGGAGTGG CAAGTACATC ACAAGATATG 
CATACTTATG CTGCCTGCAC ATTTTTGGCT GCAAGTATGA AAGAAGGGAA GCAAGGAATT 
CAGAGAAATC AAGAGTCTGT TCAGCTTGGA GCGATTGAGG CCTGTGTGAT GTGGCTACTA 
GAAAATGAAT TCATCCAGAG TACAGAAGCC AGTGATGGAA CAGAAGGAAA GGTGTATCAT 
CCAACACATC TTGGTTCGGC CACTCTTTCT TCTTCACTTT CTCCAGCTGA TACTTTAGAT 
ATTTTTGCTG ACCTGCAAAG AGCAATGAAG GGCTTTGTTT TAGAGAATGA TCTTCATATT 
CTCTATCTGG TTACACCTAT GTTTGAGGAT TGGACTACTA TTGATTGGTA TCGATTTTTC 
TGTTTATGGG AGAAGTTGCC AACTTCAATG AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA 
GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA AAAGTAGTAG CCAGAACTGA GAGACAGCAT 
CGACAAATGG CCATCCATAA AAGGTTTTTC ACCAGTCTTG TGCTATTAGA TTTAATCAGT 
GAAGTTCCCT TAAGGGAAAT AAATCAGAAA TATGGATGCA ATCGTGGGCA GATTCAATCT 
TTGCAACAGT CAGCTGCTGT TTATGCAGGG ATGATTACAG TATTTTCCAA CCGTCTGGGC 
TGGCACAACA TGGAACTACT ACTTTCCCAA TTTCAGAAGC GTCTTACGTT TGGCATCCAG 
AGGGAGCTGT GTGACCTGGT TCGGGTATCC TTACTAAATG CTCAGAGAGC CAGGGTTCTC 
TATGCTTCTG GCTTTCATAC TGTGGCAGAC CTTGCTAGAG CAAATATTGT GGAGGTGGAG 
GTGATTCTGA AAAATGCTGT GCCTTTCAAA AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG 
GAAGCAGTTG AAGAACGTCG CAATATGCGA ACTATCTGGG TGACTGGCAG AAAAGGTTTA 
ACTGAAAGGG AAGCAGCAGC CCTTATAGTG GAAGAAGCCA GAATGATTCT GCAGCAGGAC 
TTAGTTGAAA TGGGAGTGCA ATGGAATCCA TGTGCCCTGT TACATTCTAG TACATGCTCA 
TTGACTCATA GTGAGTCCGA AGTAAAGGAA CACACATTTA TATCCCAAAC TAAGAGTTCT 
TATAAAAAAT TAACATCAAA GAACAAAAGT AACACAATAT TTAGTGATTC TTATATTAAG 
CATTCACCAA ATATAGTGCA AGACTTAAAT AAAAGTAGAG AGCATACAAG TTCCTTTAAT 
TGTAATTTCC AGAATGGGAA TCAAGAACAT CAGACATGTT CCATTTTCAG AGCAAGAAAA 
CGGGCCTCTT TAGATATAAA TAAAGAGAAG CCAGGAGCCT CTCAGAATGA GGGGAAAACA 
AGTGATAAGA AAGTTGTTCA GACTTTTTCA CAGAAAACAA AAAAGGCACC TTTGAATTTC 
AATTCAGAAA AGATGAGCAG AAGTTTTCGA TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA 
TCTAGGGACA GCAGCCCCCT GAAAGACTCT GGAGCGTGTA GAATCCATTT ACAAGGACAG 
ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC CCGTTTACCT TAGATGAGAA GAAAACGGAA 
TTTAGAAATT CAGGGCCATT TGCTAAAAAT GTATCTTTGA GTGGTAAGGA AAAAGATAAT 
AAAACATCAT TCCCATTACA AATAAAGCAA AATTGTTCAT GGAACATAAC ACTAACTAAT 
GATAATTTTG TGGAGCATAT TGTCACAGGA TCTCAGAGTA AAAATGTGAC TTGTCAGGCC 
ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA GTAGCTGTTG AGGCAGAAAA AATAAATGAA 
GTGCTGATAC AAAATGGTTC AAAAAACCAG AATGTTTATA TGAAACACCA TGACATCCAT 
CCAATTAACC AGTACCTGCG AAAGCAATCT CATGAACAGA CAAGCACTAT TACCAAACAG 
AAAAATATAA TAGAGAGACA AATGCCCTGT GAAGCAGTCA GTAGTTACAT AAATAGAGAC 
TCAAATGTTA CTATCAATTG TGAAAGGATA AAGCTTAATA CAGAGGAAAA TAAACCAAGT 
CATTTTCAGG CATTAGGAGA TGATATAAGC AGAACTGTGA TACCCAGTGA AGTACTTCCA 
TCAGCTGGAG CATTTAGCAA ATCAGAAGGC CAGCATGAGA ATTTTCTAAA TATTTCTAGA 
CTACAAGAAA AAACAGGTAC TTATACAACA AACAAAACTA AAAATAATCA TGTTTCTGAC 
TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT AGTTTCTATC TGGATACTCA GTCAGAGAAA 
ATAATACAAC AGATGGCAAC TGAAAATGCC AAACTAGGAG CAAAGGACAC CAACCTGGCA 
GCAGGGATAA TGCAGAAGAG CTTAGTCCAA CAGAACTCAA TGAACTCTTT TCAGAAGGAG 
TGTCACATTC CTTTTCCTGC TGAACAGCAC CCTCTAGGAG CGACTAAGAT AGATCATTTG 
GACCTTAAGA CTGTAGGTAC TATGAAACAA AGCAGTGATT CACATGGGGT TGATATCCTG 
ACTCCAGAAA GCCCGATTTT CCATTCTCCA ATACTATTGG AGGAAAATGG TCTTTTTTTA 
AAAAAGAATG AAGTTTCTGT TACTGATTCA CAATTAAATA GTTTTCTTCA AGGTTATCAA 
ACACAAGAAA CTGTGAAACC AGTTATACTT CTGATTCCTC AAAAGAGAAC TCCCACTGGT 
GTAGAAGGAG AATGTCTTCC AGTTCCTGAA ACAAGTTTGA ATATGAGTGA TAGTTTACTA 
TTTGATAGCT TCAGTGATGA CTATCTAGTA AAAGAACAAT TACCTGATAT GCAAATGAAA 
GAACCCCTTC CTTCAGAAGT AACATCAAAC CATTTTAGTG ATTCTCTGTG TCTACAAGAA 
GACCTAATTA AAAAATCAAA TGTAAATGAG AATCAAGATA CCCACCAGCA GTTGACTTGT 
TCCAATGATG AATCTATTAT ATTTTCAGAA ATGGATTCTG TTCAGATGGT TGAAGCTTTG 
GACAATGTGG ATATATTTCC TGTCCAAGAG AAGAATCATA CTGTAGTATC TCCTAGAGCA 
TTAGAACTAA GTGATCCAGT ACTTGATGAG CACCACCAAG GTGATCAAGA TGGAGGAGAT 
CAAGATGAAA GGGCTGAAAA ATCAAAATTA ACTGGGACCA GGCAAAATCA TTCATTCATA 
TGGTCAGGGG CATCATTTGA TCTAAGTCCA GGACTGCAAA GGATTTTAGA TAAAGTATCC 
AGTCCTCTAG AAAATGAAAA GCTAAAATCA ATGACTATAA ACTTTTCCAG TTTGAATAGA 
AAAAATACAG AGTTAAATGA AGAACAAGAA GTTATTTCAA ACTTGGAGAC AAAACAAGTG 
CAGGGAATTT CATTTTCTTC TAATAATGAA GTAAAAAGCA AGATTGAGAT GCTAGAAAAC 
AATGCCAATC ATGATGAAAC CTCATCCCTC TTACCTCGTA AAGAAAGTAA TATAGTTGAT 
GATAATGGTC TCATTCCTCC TACACCCATT CCAACATCTG CTTCTAAGCT GACATTTCCA 
GGGATTCTTG AAACACCTGT AAACCCTTGG AAAACTAATA ATGTTTTACA ACCTGGTGAA 
AGTTATTTAT TTGGCTCACC TTCAGATATT AAAAACCACG ATTTAAGTCC AGGGAGTAGA 
AATGGGTTCA AAGACAACAG CCCTATTAGT GACACAAGCT TTTCACTTCA GTTATCACAG 
GATGGATTAC AGTTAACTCC AGCCTCAAGC AGTTCAGAAA GTTTGTCCAT AATTGATGTA 
GCAAGTGACC AAAATCTTTT CCAAACATTC ATTAAGGAGT GGCGGTGCAA AAAGCGATTT 
TCCATCTCAC TGGCTTGTGA AAAGATTAGA AGTTTGACAT CTTCTAAAAC TGCTACTATT 
GGCAGTAGGT TTAAGCAAGC TAGCTCACCT CAGGAAATTC CTATTAGAGA TGATGGATTT 
CCCATTAAAG GTTGTGATGA CACCTTGGTG GTTGGACTGG CAGTATGCTG GGGTGGAAGG 
GATGCCTATT ATTTTTCACT GCAGAAGGAA CAAAAGCATT CTGAAATTAG TGCCAGTTTG 
GTTCCACCTT CTTTAGATCC AAGCCTGACT TTGAAAGACA GGATGTGGTA CCTTCAATCT 
TGCTTGCGAA AGGAATCTGA TAAAGAATGT TCTGTTGTCA TCTATGACTT CATCCAGAGC 
TATAAAATTC TTCTTCTTTC TTGTGGCATC TCCTTGGAGC AAAGTTATGA AGATCCTAAG 
GTGGCATGCT GGTTACTAGA TCCAGATTCT CAGGAGCCGA CTCTTCATAG CATAGTTACC 
AGTTTTCTTC CTCATGAGCT TCCACTCCTA GAAGGGATGG AGACCAGCCA AGGGATTCAA 
AGCCTGGGGC TAAATGCTGG CAGTGAGCAT TCTGGGCGAT ACAGAGCATC TGTGGAGTCC 
ATTCTCATCT TCAACTCTAT GAATCAGCTC AACTCTTTGT TGCAGAAGGA AAACCTTCAA 
GATGTTTTCC GTAAGGTGGA AATGCCCTCT CAGTACTGCT TGGCCTTGCT AGAACTAAAT 
GGAATTGGCT TTAGTACTGC AGAATGTGAA AGTCAGAAAC ATATAATGCA AGCCAAGCTG 
GATGCAATTG AGACCCAGGC CTATCAACTA GCTGGCCACA GTTTTTCTTT CACCAGTTCA 
GATGACATCG CTGAGGTTTT ATTTTTGGAA TTGAAGTTGC CCCCAAATAG AGAGATGAAA 
AACCAAGGCA GCAAGAAAAC TCTGGGTTCT ACCAGAAGAG GGATTGACAA TGGACGCAAG 
CTAAGGCTGG GAAGACAGTT CAGCACTAGT AAGGACGTTT TAAATAAATT AAAGGCATTA 
CATCCTTTAC CAGGCTTGAT ATTAGAATGG AGAAGAATCA CTAATGCTAT TACCAAAGTG 
GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT AATCCTTTTC TTGGAATGGA AAGAATCTAT 
CCTGTATCAC AGTCGCACAC TGCTACAGGA CGAATAACCT TTACAGAACC AAATATTCAG 
AATGTGCCAA GAGATTTTGA AATCAAAATG CCAACACTAG TAGGAGAAAG CCCACCTTCT 
CAAGCTGTAG GCAAAGGCCT ACTTCCCATG GGCAGAGGAA AATATAAGAA GGGTTTCAGC 
GTGAATCCTA GATGCCAGGC ACAGATGGAG GAGAGAGCTG CAGACAGAGG AATGCCATTT 
TCAATTAGCA TGCGACATGC CTTTGTGCCT TTCCCAGGTG GTTCAATACT GGCTGCTGAC 
TACTCTCAGC TTGAACTGAG GATCTTGGCT CATTTATCCC ATGATCGTCG TCTCATTCAA 
GTGTTAAACA CTGGAGCTGA TGTTTTCAGG AGCATTGCAG CAGAGTGGAA GATGATTGAG 
CCAGAGTCTG TTGGGGATGA TCTGAGGCAG CAGGCAAAAC AGATTTGCTA TGGGATCATT 
TATGGAATGG GAGCTAAATC TTTGGGAGAG CAGATGGGCA TTAAAGAAAA TGATGCTGCA 
TGCTATATTG ACTCCTTCAA ATCCAGATAC ACAGGGATTA ATCAATTCAT GACAGAGACA 
GTGAAGAATT GTAAAAGAGA CGGATTTGTT CAGACCATTT TGGGAAGGCG TAGATATTTG 
CCAGGAATCA AAGACAACAA CCCTTATCGT AAAGCTCACG CTGAGCGTCA AGCTATCAAC 
ACAATAGTCC AAGGATCAGC AGCTGATATT GTCAAAATAG CCACAGTTAA CATTCAGAAG 
CAATTAGAGA CCTTCCACTC AACCTTCAAA TCCCATGGTC ATCGAGAGGG TATGCTCCAA 
AGTGACCAAA CAGTAGGATT GTCACGAAAG AGAAAACTGC AAGGGATGTT CTGCCCAATC 
AGAGGAGGCT TCTTCATCCT TCAACTCCAT GATGAACTCC TATATGAAGT GGCAGAAGAA 
GATGTTGTTC AGGTAGCTCA GATTGTCAAG AATGAAATGG AAAGTGCTGT AAAACTGTCT 
GTGAAATTGA AAGTGAAAGT GAAAATAGGC GCCAGCTGGG GAGAGCTAAA GGACTTTGAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861054.1
CDS	152..6757
Translation	MDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTVGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

Target ORF information:

RefSeq Version	XM_017005565.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase theta (POLQ), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005565.1

ORF Insert Sequence:

ATGGATCAGT TAAGACGTTT GCCTTCAGGA CTGGACTCTG TATTACAGAA AACTGTACCA 
TGGGGAGTAG CATTTCATCA TGCAGGTCTT ACTTTTGAGG AGAGGGATAT CATTGAAGGA 
GCCTTTCGTC AAGGTCTCAT TCGGGTCTTG GCGGCAACTT CTACTCTTTC TTCTGGGGTG 
AATTTACCTG CACGTCGTGT GATTATTCGA ACCCCTATTT TTGGTGGTCG ACCTCTAGAT 
ATTCTTACTT ATAAGCAGAT GGTTGGCCGT GCTGGCAGGA AAGGAGTGGA CACAGTAGGC 
GAGAGTATCT TAATTTGTAA GAACTCTGAG AAATCAAAAG GCATAGCTCT CCTTCAGGGT 
TCTCTAAAGC CTGTTCGCAG CTGTCTGCAA AGACGAGAAG GAGAAGAAGT AACTGGCAGC 
ATGATACGAG CTATTCTGGA GATAATAGTT GGTGGAGTGG CAAGTACATC ACAAGATATG 
CATACTTATG CTGCCTGCAC ATTTTTGGCT GCAAGTATGA AAGAAGGGAA GCAAGGAATT 
CAGAGAAATC AAGAGTCTGT TCAGCTTGGA GCGATTGAGG CCTGTGTGAT GTGGCTACTA 
GAAAATGAAT TCATCCAGAG TACAGAAGCC AGTGATGGAA CAGAAGGAAA GGTGTATCAT 
CCAACACATC TTGGTTCGGC CACTCTTTCT TCTTCACTTT CTCCAGCTGA TACTTTAGAT 
ATTTTTGCTG ACCTGCAAAG AGCAATGAAG GGCTTTGTTT TAGAGAATGA TCTTCATATT 
CTCTATCTGG TTACACCTAT GTTTGAGGAT TGGACTACTA TTGATTGGTA TCGATTTTTC 
TGTTTATGGG AGAAGTTGCC AACTTCAATG AAAAGGGTGG CAGAGCTAGT GGGAGTTGAA 
GAGGGGTTCT TGGCCCGTTG TGTGAAAGGA AAAGTAGTAG CCAGAACTGA GAGACAGCAT 
CGACAAATGG CCATCCATAA AAGGTTTTTC ACCAGTCTTG TGCTATTAGA TTTAATCAGT 
GAAGTTCCCT TAAGGGAAAT AAATCAGAAA TATGGATGCA ATCGTGGGCA GATTCAATCT 
TTGCAACAGT CAGCTGCTGT TTATGCAGGG ATGATTACAG TATTTTCCAA CCGTCTGGGC 
TGGCACAACA TGGAACTACT ACTTTCCCAA TTTCAGAAGC GTCTTACGTT TGGCATCCAG 
AGGGAGCTGT GTGACCTGGT TCGGGTATCC TTACTAAATG CTCAGAGAGC CAGGGTTCTC 
TATGCTTCTG GCTTTCATAC TGTGGCAGAC CTTGCTAGAG CAAATATTGT GGAGGTGGAG 
GTGATTCTGA AAAATGCTGT GCCTTTCAAA AGTGCCCGGA AGGCAGTGGA TGAGGAAGAG 
GAAGCAGTTG AAGAACGTCG CAATATGCGA ACTATCTGGG TGACTGGCAG AAAAGGTTTA 
ACTGAAAGGG AAGCAGCAGC CCTTATAGTG GAAGAAGCCA GAATGATTCT GCAGCAGGAC 
TTAGTTGAAA TGGGAGTGCA ATGGAATCCA TGTGCCCTGT TACATTCTAG TACATGCTCA 
TTGACTCATA GTGAGTCCGA AGTAAAGGAA CACACATTTA TATCCCAAAC TAAGAGTTCT 
TATAAAAAAT TAACATCAAA GAACAAAAGT AACACAATAT TTAGTGATTC TTATATTAAG 
CATTCACCAA ATATAGTGCA AGACTTAAAT AAAAGTAGAG AGCATACAAG TTCCTTTAAT 
TGTAATTTCC AGAATGGGAA TCAAGAACAT CAGACATGTT CCATTTTCAG AGCAAGAAAA 
CGGGCCTCTT TAGATATAAA TAAAGAGAAG CCAGGAGCCT CTCAGAATGA GGGGAAAACA 
AGTGATAAGA AAGTTGTTCA GACTTTTTCA CAGAAAACAA AAAAGGCACC TTTGAATTTC 
AATTCAGAAA AGATGAGCAG AAGTTTTCGA TCTTGGAAAC GTAGAAAGCA TCTAAAGCGA 
TCTAGGGACA GCAGCCCCCT GAAAGACTCT GGAGCGTGTA GAATCCATTT ACAAGGACAG 
ACTCTGTCTA ATCCTAGTCT TTGTGAAGAC CCGTTTACCT TAGATGAGAA GAAAACGGAA 
TTTAGAAATT CAGGGCCATT TGCTAAAAAT GTATCTTTGA GTGGTAAGGA AAAAGATAAT 
AAAACATCAT TCCCATTACA AATAAAGCAA AATTGTTCAT GGAACATAAC ACTAACTAAT 
GATAATTTTG TGGAGCATAT TGTCACAGGA TCTCAGAGTA AAAATGTGAC TTGTCAGGCC 
ACTAGTGTGG TTAGTGAAAA GGGCAGAGGA GTAGCTGTTG AGGCAGAAAA AATAAATGAA 
GTGCTGATAC AAAATGGTTC AAAAAACCAG AATGTTTATA TGAAACACCA TGACATCCAT 
CCAATTAACC AGTACCTGCG AAAGCAATCT CATGAACAGA CAAGCACTAT TACCAAACAG 
AAAAATATAA TAGAGAGACA AATGCCCTGT GAAGCAGTCA GTAGTTACAT AAATAGAGAC 
TCAAATGTTA CTATCAATTG TGAAAGGATA AAGCTTAATA CAGAGGAAAA TAAACCAAGT 
CATTTTCAGG CATTAGGAGA TGATATAAGC AGAACTGTGA TACCCAGTGA AGTACTTCCA 
TCAGCTGGAG CATTTAGCAA ATCAGAAGGC CAGCATGAGA ATTTTCTAAA TATTTCTAGA 
CTACAAGAAA AAACAGGTAC TTATACAACA AACAAAACTA AAAATAATCA TGTTTCTGAC 
TTAGGTTTAG TCCTCTGTGA TTTTGAAGAT AGTTTCTATC TGGATACTCA GTCAGAGAAA 
ATAATACAAC AGATGGCAAC TGAAAATGCC AAACTAGGAG CAAAGGACAC CAACCTGGCA 
GCAGGGATAA TGCAGAAGAG CTTAGTCCAA CAGAACTCAA TGAACTCTTT TCAGAAGGAG 
TGTCACATTC CTTTTCCTGC TGAACAGCAC CCTCTAGGAG CGACTAAGAT AGATCATTTG 
GACCTTAAGA CTGTAGGTAC TATGAAACAA AGCAGTGATT CACATGGGGT TGATATCCTG 
ACTCCAGAAA GCCCGATTTT CCATTCTCCA ATACTATTGG AGGAAAATGG TCTTTTTTTA 
AAAAAGAATG AAGTTTCTGT TACTGATTCA CAATTAAATA GTTTTCTTCA AGGTTATCAA 
ACACAAGAAA CTGTGAAACC AGTTATACTT CTGATTCCTC AAAAGAGAAC TCCCACTGGT 
GTAGAAGGAG AATGTCTTCC AGTTCCTGAA ACAAGTTTGA ATATGAGTGA TAGTTTACTA 
TTTGATAGCT TCAGTGATGA CTATCTAGTA AAAGAACAAT TACCTGATAT GCAAATGAAA 
GAACCCCTTC CTTCAGAAGT AACATCAAAC CATTTTAGTG ATTCTCTGTG TCTACAAGAA 
GACCTAATTA AAAAATCAAA TGTAAATGAG AATCAAGATA CCCACCAGCA GTTGACTTGT 
TCCAATGATG AATCTATTAT ATTTTCAGAA ATGGATTCTG TTCAGATGGT TGAAGCTTTG 
GACAATGTGG ATATATTTCC TGTCCAAGAG AAGAATCATA CTGTAGTATC TCCTAGAGCA 
TTAGAACTAA GTGATCCAGT ACTTGATGAG CACCACCAAG GTGATCAAGA TGGAGGAGAT 
CAAGATGAAA GGGCTGAAAA ATCAAAATTA ACTGGGACCA GGCAAAATCA TTCATTCATA 
TGGTCAGGGG CATCATTTGA TCTAAGTCCA GGACTGCAAA GGATTTTAGA TAAAGTATCC 
AGTCCTCTAG AAAATGAAAA GCTAAAATCA ATGACTATAA ACTTTTCCAG TTTGAATAGA 
AAAAATACAG AGTTAAATGA AGAACAAGAA GTTATTTCAA ACTTGGAGAC AAAACAAGTG 
CAGGGAATTT CATTTTCTTC TAATAATGAA GTAAAAAGCA AGATTGAGAT GCTAGAAAAC 
AATGCCAATC ATGATGAAAC CTCATCCCTC TTACCTCGTA AAGAAAGTAA TATAGTTGAT 
GATAATGGTC TCATTCCTCC TACACCCATT CCAACATCTG CTTCTAAGCT GACATTTCCA 
GGGATTCTTG AAACACCTGT AAACCCTTGG AAAACTAATA ATGTTTTACA ACCTGGTGAA 
AGTTATTTAT TTGGCTCACC TTCAGATATT AAAAACCACG ATTTAAGTCC AGGGAGTAGA 
AATGGGTTCA AAGACAACAG CCCTATTAGT GACACAAGCT TTTCACTTCA GTTATCACAG 
GATGGATTAC AGTTAACTCC AGCCTCAAGC AGTTCAGAAA GTTTGTCCAT AATTGATGTA 
GCAAGTGACC AAAATCTTTT CCAAACATTC ATTAAGGAGT GGCGGTGCAA AAAGCGATTT 
TCCATCTCAC TGGCTTGTGA AAAGATTAGA AGTTTGACAT CTTCTAAAAC TGCTACTATT 
GGCAGTAGGT TTAAGCAAGC TAGCTCACCT CAGGAAATTC CTATTAGAGA TGATGGATTT 
CCCATTAAAG GTTGTGATGA CACCTTGGTG GTTGGACTGG CAGTATGCTG GGGTGGAAGG 
GATGCCTATT ATTTTTCACT GCAGAAGGAA CAAAAGCATT CTGAAATTAG TGCCAGTTTG 
GTTCCACCTT CTTTAGATCC AAGCCTGACT TTGAAAGACA GGATGTGGTA CCTTCAATCT 
TGCTTGCGAA AGGAATCTGA TAAAGAATGT TCTGTTGTCA TCTATGACTT CATCCAGAGC 
TATAAAATTC TTCTTCTTTC TTGTGGCATC TCCTTGGAGC AAAGTTATGA AGATCCTAAG 
GTGGCATGCT GGTTACTAGA TCCAGATTCT CAGGAGCCGA CTCTTCATAG CATAGTTACC 
AGTTTTCTTC CTCATGAGCT TCCACTCCTA GAAGGGATGG AGACCAGCCA AGGGATTCAA 
AGCCTGGGGC TAAATGCTGG CAGTGAGCAT TCTGGGCGAT ACAGAGCATC TGTGGAGTCC 
ATTCTCATCT TCAACTCTAT GAATCAGCTC AACTCTTTGT TGCAGAAGGA AAACCTTCAA 
GATGTTTTCC GTAAGGTGGA AATGCCCTCT CAGTACTGCT TGGCCTTGCT AGAACTAAAT 
GGAATTGGCT TTAGTACTGC AGAATGTGAA AGTCAGAAAC ATATAATGCA AGCCAAGCTG 
GATGCAATTG AGACCCAGGC CTATCAACTA GCTGGCCACA GTTTTTCTTT CACCAGTTCA 
GATGACATCG CTGAGGTTTT ATTTTTGGAA TTGAAGTTGC CCCCAAATAG AGAGATGAAA 
AACCAAGGCA GCAAGAAAAC TCTGGGTTCT ACCAGAAGAG GGATTGACAA TGGACGCAAG 
CTAAGGCTGG GAAGACAGTT CAGCACTAGT AAGGACGTTT TAAATAAATT AAAGGCATTA 
CATCCTTTAC CAGGCTTGAT ATTAGAATGG AGAAGAATCA CTAATGCTAT TACCAAAGTG 
GTCTTTCCCC TTCAGCGGGA AAAGTGTCTT AATCCTTTTC TTGGAATGGA AAGAATCTAT 
CCTGTATCAC AGTCGCACAC TGCTACAGGA CGAATAACCT TTACAGAACC AAATATTCAG 
AATGTGCCAA GAGATTTTGA AATCAAAATG CCAACACTAG TAGGAGAAAG CCCACCTTCT 
CAAGCTGTAG GCAAAGGCCT ACTTCCCATG GGCAGAGGAA AATATAAGAA GGGTTTCAGC 
GTGAATCCTA GATGCCAGGC ACAGATGGAG GAGAGAGCTG CAGACAGAGG AATGCCATTT 
TCAATTAGCA TGCGACATGC CTTTGTGCCT TTCCCAGGTG GTTCAATACT GGCTGCTGAC 
TACTCTCAGC TTGAACTGAG GATCTTGGCT CATTTATCCC ATGATCGTCG TCTCATTCAA 
GTGTTAAACA CTGGAGCTGA TGTTTTCAGG AGCATTGCAG CAGAGTGGAA GATGATTGAG 
CCAGAGTCTG TTGGGGATGA TCTGAGGCAG CAGGCAAAAC AGATTTGCTA TGGGATCATT 
TATGGAATGG GAGCTAAATC TTTGGGAGAG CAGATGGGCA TTAAAGAAAA TGATGCTGCA 
TGCTATATTG ACTCCTTCAA ATCCAGATAC ACAGGGATTA ATCAATTCAT GACAGAGACA 
GTGAAGAATT GTAAAAGAGA CGGATTTGTT CAGACCATTT TGGGAAGGCG TAGATATTTG 
CCAGGAATCA AAGACAACAA CCCTTATCGT AAAGCTCACG CTGAGCGTCA AGCTATCAAC 
ACAATAGTCC AAGGATCAGC AGCTGATATT GTCAAAATAG CCACAGTTAA CATTCAGAAG 
CAATTAGAGA CCTTCCACTC AACCTTCAAA TCCCATGGTC ATCGAGAGGG TATGCTCCAA 
AGTGACCAAA CAGTAGGATT GTCACGAAAG AGAAAACTGC AAGGGATGTT CTGCCCAATC 
AGAGGAGGCT TCTTCATCCT TCAACTCCAT GATGAACTCC TATATGAAGT GGCAGAAGAA 
GATGTTGTTC AGGTAGCTCA GATTGTCAAG AATGAAATGG AAAGTGCTGT AAAACTGTCT 
GTGAAATTGA AAGTGAAAGT GAAAATAGGC GCCAGCTGGG GAGAGCTAAA GGACTTTGAT 
GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

One-step enzymatic modification of RNA 3' termini using polymerase θ.
Nucleic acids research47(7)3272-3283(2019 04)
Thomas C,Rusanov T,Hoang T,Augustin T,Kent T,Gaspar I,Pomerantz RT

DNA polymerase θ accomplishes translesion synthesis opposite 1,N
Genes & development33(5-6)282-287(2019 03)
Yoon JH,Johnson RE,Prakash L,Prakash S

A case of interdigitating dendritic cell sarcoma studied by whole-exome sequencing.
Genes & genomics40(12)1279-1285(2018 12)
Hong KH,Song S,Shin W,Kang K,Cho CS,Hong YT,Han K,Moon JH

Expanded Substrate Scope of DNA Polymerase θ and DNA Polymerase β: Lyase Activity on 5'-Overhangs and Clustered Lesions.
Biochemistry57(42)6119-6127(2018 10)
Laverty DJ,Greenberg MM

DNA Polymerase θ Increases Mutational Rates in Mitochondrial DNA.
ACS chemical biology13(4)900-908(2018 04)
Wisnovsky S,Sack T,Pagliarini DJ,Laposa RR,Kelley SO

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POLQ cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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